Prakash Ghosh, Rajashree Chowdhury, Khaledul Faisal, Md Anik Ashfaq Khan, Faria Hossain, Md Abu Rahat, Md Arko Ayon Chowdhury, Nishad Tasnim Mithila, Mostafa Kamal, Shomik Maruf, Rupen Nath, Rea Maja Kobialka, Arianna Ceruti, Mary Cameron, Malcolm S Duthie, Ahmed Abd El Wahed, Dinesh Mondal
A rapid, cost-effective, and simple nucleic acid isolation technique coupled with a point-of-need DNA amplification assay is a desirable goal for programmatic use. For diagnosis of Visceral Leishmaniasis (VL), Recombinase Polymerase Amplification (RPA) rapid tests for the detection of Leishmania DNA are versatile and have operational advantages over qPCR. To facilitate the delivery of the RPA test at point-of-need for VL diagnosis, we compared two rapid DNA extraction methods, SwiftDx (SX) and an in-house Boil and Spin (BS) method, coupled with RPA amplification, versus more widely used methods for DNA extraction and amplification, namely Qiagen (Q) kits and qPCR, respectively...
December 11, 2023: Diagnostics