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https://www.readbyqxmd.com/read/28867354/development-of-a-simple-and-economical-diagnostic-test-for-canine-leishmaniasis
#1
Valeria S García, Verónica D G Gonzalez, Luis Gugliotta, Alexis Burna, Ana Demonte, Diego G Arias, Matías S Cabeza, Sergio A Guerrero
Visceral leishmaniasis is a public health problem worldwide. The early diagnosis in dogs is crucial, since they are an epidemiologically relevant reservoir of the disease. The aim of a field study is to early identify the disease allowing rapid intervention to reduce its effects. We propose an immunoagglutination test as a visual in situ method for diagnosis of canine visceral leishmaniasis. Latex-protein complexes were sensitized by covalent coupling of a chimeric recombinant antigen of Leishmania spp. onto polystyrene latex with carboxyl functionality...
September 1, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28844893/tandem-affinity-purification-of-exosome-and-replication-factor-c-complexes-from-the-non-human-infectious-kinetoplastid-parasite-crithidia-fasciculata
#2
Wakisa Kipandula, Terry K Smith, Stuart A MacNeill
Kinetoplastid parasites are responsible for a range of diseases with significant global impact. Trypanosoma brucei and Trypanosoma cruzi cause human African trypanosomiasis and Chagas disease, respectively, while various Leishmania species are responsible for cutaneous, mucocutaneous and visceral leishmaniasis. Understanding the biology of these organisms is key for effective diagnosis, prophylaxis and treatment. The insect parasite Crithidia fasciculata offers a safe and low-cost alternative for studies of kinetoplastid biology...
August 24, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28843396/profiling-gene-expression-of-antimony-response-genes-in-leishmania-viannia-panamensis-and-infected-macrophages-and-its-relationship-with-drug-susceptibility
#3
Maria Claudia Barrera, Laura Jimena Rojas, Austin Weiss, Olga Fernandez, Diane McMahon-Pratt, Nancy G Saravia, Maria Adelaida Gomez
The mechanisms of Leishmania resistance to antimonials have been primarily determined in experimentally derived Leishmania strains. However, their participation in the susceptibility phenotype in field isolates has not been conclusively established. Being an intracellular parasite, the activity of antileishmanials is dependent on internalization of drugs into host cells and effective delivery to the intracellular compartments inhabited by the parasite. In this study we quantified and comparatively analyzed the gene expression of nine molecules involved in mechanisms of xenobiotic detoxification and Leishmania resistance to antimonial drugs in resistant and susceptible laboratory derived and clinical L...
August 24, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28841834/cutaneous-leishmaniasis-in-a-severely-immunocompromised-hiv-patient-in-kumbo-northwest-region-of-cameroon-case-report
#4
Larry N Tangie, A Desmond, Leopold N Aminde, Annabel M Ako, P M Halle
BACKGROUND: Leishmaniasis is a rising opportunistic infection in individuals with human immunodeficiency virus (HIV). Cases of leishmania and HIV co-infection have been documented in several countries in the world with most reporting on the association between visceral leishmaniasis (VL) and HIV. We herein report the case of cutaneous leishmaniasis (CL) occurring in an HIV seropositive patient. CASE PRESENTATION: A 28 year old Cameroonian female diagnosed with HIV for 6 months earlier, presented to our facility with a 3 months history of non-painful rash...
August 25, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28820895/identification-of-leishmania-donovani-antigen-in-circulating-immune-complexes-of-visceral-leishmaniasis-subjects-for-diagnosis
#5
Fauzia Jamal, Pushkar Shivam, Sarita Kumari, Manish Kumar Singh, Abul Hasan Sardar, Pushpanjali, Selvasankar Murugesan, Shyam Narayan, Anil Kumar Gupta, Krishna Pandey, V N R Das, Vahab Ali, Sanjiva Bimal, Pradeep Das, Shubhankar K Singh
The unreliability of most of the existing antibody-based diagnostic kits to discriminate between active and treated VL cases, relapse situation and reinfection are a major hurdle in controlling the cases of Kala-azar in an endemic area. An antigen targeted diagnostic approaches can be an attractive strategy to overcome these problems. Hence, this study was focused on identifying the Leishmania antigens, lies in circulating immune complex (CICs), can be used for diagnostic as well as prognostic purposes. The present study was conducted on peripheral blood samples of 115 human subjects, based on isolation of CICs...
2017: PloS One
https://www.readbyqxmd.com/read/28810997/cutaneous-leishmaniasis-in-a-saudi-arabian-soldier-stationed-in-the-united-states
#6
Kelly Laurent, Jason Susong, Eric Fillman, Simon Ritchie
BACKGROUND: Leishmaniasis is a common parasitic disease seen in many parts of the world, especially in areas where current U.S. and international forces are deployed. Approximately 350 million people are thought to be at risk of cutaneous leishmaniasis (CL) with an annual incidence of 1.5 million cases. Over 90% of cutaneous infections with Leishmania occur in the Middle East, Brazil, and Peru. Outbreaks of CL may occur in military personnel deployed to endemic areas. Since the incubation period for symptomatic CL ranges from weeks to months, symptoms may not appear until well after returning to the United States...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28807318/application-of-a-specific-quantitative-real-time-pcr-qpcr-to-identify-leishmania-infantum-dna-in-spleen-skin-and-hair-samples-of-wild-leporidae
#7
María Victoria Ortega, Inmaculada Moreno, Mercedes Domínguez, María Luisa de la Cruz, Ana Belén Martín, Antonio Rodríguez-Bertos, Raúl López, Alejandro Navarro, Sergio González, María Mazariegos, Joaquín Goyache, Lucas Domínguez, Nerea García
The aim of this study was to compare a quantitative real-time PCR (qPCR) validated for the detection of Leishmania infantum in dogs with a nested PCR but in wild Leporidae. Additionally, L. infantum results from indirect immunofluorescent antibody test (IFAT) and in vitro culture were also compared with qPCR. Different samples (spleen, skin and hair) recovered from 224 European rabbits and 70 Iberian hares from two green areas of Madrid Council were analysed for the detection of L. infantum. The presence of Leishmania kDNA was detected by qPCR in 58 out of 221 (26...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28795077/sensitive-molecular-diagnostics-for-cutaneous-leishmaniasis
#8
Orli Sagi, Anat Berkowitz, Shlomi Codish, Victor Novack, Aviv Rashti, Fouad Akad, Yonat Shemer-Avni
BACKGROUND: Rapid diagnosis of cutaneous leishmaniasis (CL) and identification of Leishmania species is highly important for the disease management. In Israel, CL is caused mainly by Leishmania major and Leishmania tropica species. METHODS: We established an easy to handle point of care lesion-swabbing, combined with a highly sensitive multiplex real time PCR (multiplex qPCR) for accurate and rapid diagnosis of Leishmania species. RESULTS: Using three probes: one general for: Leishmania species, and two specific for L major, and L tropica, we screened 1783 clinical samples collected during two years...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28776770/cutaneous-leishmaniasis-by-leishmania-infantum-behind-granulomatous-lesions-of-unknown-aetiology
#9
G Merino-Espinosa, V Corpas-López, V Díaz-Sáez, F Morillas-Márquez, J Tercedor-Sánchez, J M Azaña-Defez, J López-Hidalgo, J Aneiros-Fernández, J Martín-Sánchez
BACKGROUND: Cutaneous leishmaniasis (CL) is underestimated in Spain as in other European countries due to the polymorphism of its clinical manifestations and histopathological features discouraging doctors from suspecting leishmaniasis. Mucosal manifestations (ML) are misdiagnosed due to the fact that they often mimic cancer. OBJECTIVES: Given that leishmaniasis may be masked as different granulomatous diseases in Leishmania infantum endemic areas, the aim of this study was to verify this misdiagnosing and contributes to the improvement of CL/ML diagnosis...
August 3, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28761480/detection-of-potentially-diagnostic-leishmania-antigens-with-western-blot-analysis-of-sera-from-patients-with-cutaneous-and-visceral-leishmaniases
#10
Seyyed Javad Seyyedtabaei, Ali Rostami, Ali Haghighi, Mehdi Mohebali, Bahram Kazemi, Shirzad Fallahi, Adel Spotin
BACKGROUND: Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS: This study was performed from 2010-2014 and participants were different parts of Iran. Serum samples were obtained from 43 patients with proven CL, 33 patients with proven VL, 39 patients with other parasitic diseases and 23 healthy individuals...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28758598/synthetic-peptides-derived-from-ribosomal-proteins-of-leishmania-spp-in-mucocutaneous-leishmaniasis-diagnostic-usefulness
#11
Magda Melissa Florez, Camila Indiani de Oliveira, Concepción Puerta, Fanny Guzmán, Martha Ayala, Gladis Montoya, Gabriela Delgado
The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA; 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28735574/multicentre-evaluation-of-a-direct-agglutination-test-prototype-kit-dat-lpc-for-diagnosis-of-visceral-leishmaniasis
#12
E Oliveira, D Oliveira, F A Cardoso, J R Barbosa, A P Marcelino, T Dutra, T Araujo, L Fernandes, D Duque, A Rabello
In this study, we assessed the sensitivity, specificity, and diagnostic accuracy of a previously developed direct agglutination test (DAT) using a freeze-dried antigen derived from Leishmania infantum promastigotes and composed in a prototype kit for visceral leishmaniasis (VL) diagnosis, named DAT-LPC. To evaluate DAT-LPC reproducibility, the kit was used to analyse 207 serum samples from VL patients and 80 serum samples from patients with other parasitic infections or healthy subjects in four laboratories from different public health institutions in Brazil...
July 24, 2017: Parasitology
https://www.readbyqxmd.com/read/28727723/analytical-sensitivity-and-specificity-of-a-loop-mediated-isothermal-amplification-lamp-kit-prototype-for-detection-of-trypanosoma-cruzi-dna-in-human-blood-samples
#13
Susana A Besuschio, Mónica Llano Murcia, Alejandro F Benatar, Severine Monnerat, Israel Cruz, Albert Picado, María de Los Ángeles Curto, Yutaka Kubota, Diana P Wehrendt, Paula Pavia, Yasuyoshi Mori, Concepción Puerta, Joseph M Ndung'u, Alejandro G Schijman
This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside of the caps. The reaction is carried out at 65°C during 40 minutes. Calcein allows direct detection of amplified products with the naked eye. Inclusivity and selectivity were tested in purified DNA from Trypanosoma cruzi stocks belonging to the six discrete typing units (DTUs), in DNA from other protozoan parasites and in human DNA...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28722607/mucosal-leishmaniasis-a-retrospective-study-of-327-cases-from-an-endemic-area-of-leishmania-viannia-braziliensis
#14
Carolina Cincurá, Clara Mônica F de Lima, Paulo R L Machado, Jamary Oliveira-Filho, Marshall J Glesby, Marcus M Lessa, Edgar M Carvalho
Mucosal leishmaniasis (ML) is observed only in about 3% of patients with American tegumentary leishmaniasis (ATL) but has a high potential for destructive, disfiguring, and disabling sequelae. Prior reports of clinical and epidemiologic features of ML are limited by small numbers of cases. In this study, we evaluated changes in the demographic features and clinical presentation of ML in an endemic area of Leishmania braziliensis transmission over a period of 20 years. The charts of 327 patients with ML diagnosed between 1995 and 2014 were reviewed...
July 10, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28705248/prevention-of-feline-leishmaniosis-with-an-imidacloprid-10-flumethrin-4-5-polymer-matrix-collar
#15
Emanuele Brianti, Luigi Falsone, Ettore Napoli, Gabriella Gaglio, Salvatore Giannetto, Maria Grazia Pennisi, Vito Priolo, Maria Stefania Latrofa, Viviana Domenica Tarallo, Fabrizio Solari Basano, Roberto Nazzari, Katrin Deuster, Matthias Pollmeier, Laura Gulotta, Vito Colella, Filipe Dantas-Torres, Gioia Capelli, Domenico Otranto
BACKGROUND: Leishmaniosis caused by Leishmania infantum is one of the most important vector-borne diseases affecting animals and humans worldwide. Dogs are considered main reservoirs of the zoonotic forms, though in the last years the role of cats as reservoirs has been increasingly investigated. Feline leishmaniosis (FeL) occurs in endemic areas and no specific preventive measures have been investigated so far. In this study the efficacy of a 10% imidacloprid/4.5% flumethrin polymer matrix collar, licensed for tick and flea prevention, has been assessed against FeL in a longitudinal study on 204 privately owned cats from the Aeolian islands (Sicily), an area highly endemic for the disease...
July 14, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28704463/a-novel-non-invasive-diagnostic-sampling-technique-for-cutaneous-leishmaniasis
#16
Yasaman Taslimi, Pardis Sadeghipour, Sima Habibzadeh, Vahid Mashayekhi, Hossien Mortazavi, Ingrid Müller, Majella E Lane, Pascale Kropf, Sima Rafati
Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infected tissues. Although these techniques are highly specific, they require highly skilled health workers and have the inherent risks of all invasive procedures, such as pain and risk of bacterial and fungal super-infection...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28704369/clinical-and-parasitological-factors-in-parasite-persistence-after-treatment-and-clinical-cure-of-cutaneous-leishmaniasis
#17
Alvaro J Martínez-Valencia, Carlos Frisherald Daza-Rivera, Mariana Rosales-Chilama, Alexandra Cossio, Elkin J Casadiego Rincón, Mayur M Desai, Nancy Gore Saravia, María Adelaida Gómez
BACKGROUND: The determinants of parasite persistence or elimination after treatment and clinical resolution of cutaneous leishmaniasis (CL) are unknown. We investigated clinical and parasitological parameters associated with the presence and viability of Leishmania after treatment and resolution of CL caused by L. Viannia. METHODS: Seventy patients who were treated with meglumine antimoniate (n = 38) or miltefosine (n = 32) and cured, were included in this study...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28689776/diagnostic-challenges-in-the-era-of-canine-leishmania-infantum-vaccines
#18
REVIEW
Laia Solano-Gallego, Luís Cardoso, Maria Grazia Pennisi, Christine Petersen, Patrick Bourdeau, Gaetano Oliva, Guadalupe Miró, Lluís Ferrer, Gad Baneth
The diagnosis of canine leishmaniosis (CanL) is complex due to its variable clinical manifestations and laboratory findings. The availability of vaccines to prevent CanL has increased the complexity of diagnosis, as serological tests may not distinguish between naturally infected and vaccinated dogs. Current practices of prevaccination screening are not sufficiently sensitive to detect subclinically infected dogs, resulting in the vaccination of infected animals, which may lead to disease in vaccinated dogs that are also infectious to sand flies...
September 2017: Trends in Parasitology
https://www.readbyqxmd.com/read/28687071/recurrence-of-visceral-and-muco-cutaneous-leishmaniasis-in-a-patient-under-immunosuppressive-therapy
#19
Gilles Darcis, Gert Van der Auwera, Jean-Baptiste Giot, Marie-Pierre Hayette, Françoise Tassin, Jorge Arrese Estrada, Lieselotte Cnops, Michel Moutschen, Laurence de Leval, Philippe Leonard
BACKGROUND: Leishmaniasis is a protozoan disease caused by parasites of the genus Leishmania, transmitted to humans by sandflies. The diagnosis of leishmaniasis is often challenging as it mimics many other infectious or malignant diseases. The disease can present in three ways: cutaneous, mucocutaneous, or visceral leishmaniasis, which rarely occur together or consecutively. CASE PRESENTATION: The patient was a 52 years old immunosuppressed Belgian woman with a long history of severe rheumatoid arthritis...
July 7, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28684052/rapid-detection-of-trypanosoma-cruzi-by-colorimetric-loop-mediated-isothermal-amplification-lamp-a-potential-novel-tool-for-the-detection-of-congenital-chagas-infection
#20
Rocío Rivero, Margarita Bisio, Elsa Beatriz Velázquez, Mónica Inés Esteva, Karenina Scollo, Nicolás Leonel González, Jaime Altcheh, Andrés Mariano Ruiz
Early diagnosis of congenital Trypanosomacruzi transmission in newborns is essential because babies show high indices of cure. Conventional diagnosis is based on microscopic examination and serology. Molecular diagnosis is a promising alternative to replace conventional diagnosis, although it is not well suited for adoption in laboratories with limited resources. Isothermal DNA amplification methods have the advantage of not requiring expensive equipment. The aim of this work was to apply loop-mediated isothermal amplification (LAMP) to detect congenital infection in babies colorimetrically...
June 19, 2017: Diagnostic Microbiology and Infectious Disease
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