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leishmania diagnosis

Daniel S Dias, Patrícia A F Ribeiro, Vívian T Martins, Daniela P Lage, Fernanda F Ramos, Anna L T Dias, Marcella R Rodrigues, Áquila S B Portela, Lourena E Costa, Rachel B Caligiorne, Bethina T Steiner, Miguel A Chávez-Fumagalli, Beatriz C S Salles, Thaís T O Santos, Julia A G Silveira, Danielle F Magalhães-Soares, Bruno M Roatt, Ricardo A Machado-de-Ávila, Mariana C Duarte, Daniel Menezes-Souza, Eduardo S Silva, Alexsandro S Galdino, Eduardo A F Coelho
Visceral leishmaniasis (VL) represents a serious public health problem, as Leishmania infantum is one of main disease causative agents in the Americas. In a previous immunoproteomic study, the prohibitin (PHB) protein was identified in L. infantum promastigote and amastigote extracts by antibodies in asymptomatic and symptomatic VL dog sera. This protein was found to be highly conserved between different Leishmania spp., but it presented a low identity with amino acid sequences of other organisms. The aim of the present study was to evaluate the cellular response induced by the recombinant PHB (rPHB) protein in BALB/c mice, as well as in PBMCs purified from untreated and treated VL patients, as well as to evaluate its protective efficacy against an infection by L...
November 9, 2017: Cellular Immunology
Daniel S Dias, Vívian T Martins, Patrícia A F Ribeiro, Fernanda F Ramos, Daniela P Lage, Grasiele S V Tavares, Débora V C Mendonça, Miguel A Chávez-Fumagalli, Jamil S Oliveira, Eduardo S Silva, Dawidson A Gomes, Michele A Rodrigues, Mariana C Duarte, Alexsandro S Galdino, Daniel Menezes-Souza, Eduardo A F Coelho
In this study, a Leishmania hypothetical protein, LiHyS, was evaluated regarding its antigenicity, immunogenicity and protective efficacy against visceral leishmaniasis (VL). Regarding antigenicity, immunoblottings and an enzyme-linked immunosorbent assay using human and canine sera showed high sensitivity and specificity values for the recombinant protein (rLiHyS) in the diagnosis of VL. When evaluating the immunogenicity of LiHyS, which is possibly located in the parasite's flagellar pocket, proliferative assays using peripheral blood mononuclear cells from healthy subjects or VL patients showed a high proliferative index in both individuals, when compared to the results obtained using rA2 or unstimulated cultures...
November 8, 2017: Parasitology
Najoua Haouas, Omar Amer, Fawwaz Freih Alshammri, Shorooq Al-Shammari, Latifa Remadi, Ibrahim Ashankyty
BACKGROUND: Cutaneous leishmaniasis (CL) is a vector-borne disease transmitted by the bite of an infected sand fly. This disease is highly prevalent in Saudi Arabia where Leishmania major and L. tropica are the etiological agents. In the region of Hail, northwestern of Saudi Arabia, the incidence is about 183 cases/year. However, the epidemiology of the disease in this area is not well understood. Thus, an epidemiological survey was conducted in 2015-2016 to identify the circulating parasite and the sand fly fauna in the region of Hail...
November 2, 2017: Parasites & Vectors
Darién Ledesma, Eduardo Berriatua, M Carmen Thomas, Luis Jesús Bernal, María Ortuño, Celia Benitez, Adriana Egui, Kostas Papasouliotis, Bryn Tennant, Julia Chambers, Juan José Infante, Manuel Carlos López
BACKGROUND: Leishmania infantum is a protozoan parasite transmitted by phlebotomine sand flies that causes life-threatening disease in humans and dogs. The dog is the primary reservoir of the parasite and early diagnosis of canine leishmaniosis is crucial at the clinical and epidemiological level. The currently available serological tests for CanL diagnostic show limitations therefore the aim of the present study was to investigate the diagnostic performance of an indirect antibody ELISA based on the Leishmania infantum recombinant antigen PFR1 in asymptomatically infected dogs...
October 23, 2017: BMC Veterinary Research
Tuna Demirdal, Ümmü Sena Sarı, Salih Atakan Nemli, Serap Ural, Sibel El
Visceral leishmaniasis is an endemic disease in many parts of world, and if untreated, it is a potentially life-threatening infectious disease. It is similar to chronic liver disease because of signs and symptoms such as fever, weight loss, hepatosplenomegaly, and pancytopenia. In this study, we present a case of visceral leishmaniasis, which is known to be a chronic hepatitis B infection, that was coincidentally diagnosed with liver biopsy. Visceral leishmaniasis should be considered as an infectious disease in the differential diagnosis of chronic liver diseases...
September 2017: Türkiye Parazitolojii Dergisi
Trupti A Patel, Glenis K Scadding, David E Phillips, Diana N Lockwood
Old World species of Leishmania typically cause visceral and cutaneous leishmaniasis. Mucosal involvement is typically seen with infection by Leishmania species found in South America, usually after the healing of cutaneous leishmaniasis. We present five imported cases of mucosal leishmaniasis caused by Old World Mediterranean Leishmania infantum exclusively affecting the nasal mucosa or vocal cord. In only one case was there a recollection of a preceding cutaneous lesion compatible with cutaneous Leishmaniasis...
October 2017: American Journal of Tropical Medicine and Hygiene
Lucrecia Acosta Soto, Nelson Caballero, Lesny Ruth Fuentes, Pedro Torres Muñoz, Jose Ramón Gómez Echevarría, Montserrat Pérez López, Fernando Jorge Bornay Llinares, John L Stanford, Cynthia A Stanford, Helen D Donoghue
In Central America, few cases of leprosy have been reported, but the disease may be unrecognized. Diagnosis is based on clinical criteria and histology. Preliminary field work in Nicaragua and Honduras found patients, including many children, with skin lesions clinically suggestive of atypical cutaneous leishmaniasis or indeterminate leprosy. Histology could not distinguish these diseases although acid-fast organisms were visible in a few biopsies. Lesions healed after standard antimicrobial therapy for leprosy...
October 2017: American Journal of Tropical Medicine and Hygiene
Carlos A Gomez, Indre Budvytiene, Allison J Zemek, Niaz Banaei
Background: Identification of fungi causing invasive fungal disease (IFD) is critical for guiding antifungal therapy. We describe the performance and clinical impact of a targeted panfungal PCR amplicon sequencing assay for culture-independent diagnosis of IFD. Methods: Between January 2009 and September 2016, 233 specimens, consisting of fresh and formalin-fixed paraffin embedded (FFPE) tissues, and sterile body fluids with known diagnosis of IFD based on reference method results (n=117), and specimens with negative fungal culture, but with microscopic and ancillary findings indicative of IFD (n=116), were included...
August 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Charles Abongomera, Ermias Diro, Florian Vogt, Achilleas Tsoumanis, Zelalem Mekonnen, Henok Admassu, Robert Colebunders, Rezika Mohammed, Koert Ritmeijer, Johan van Griensven
Background: East Africa, where Leishmania donovani is prevalent, faces the highest burden world-wide of visceral leishmaniasis (VL) and human immunodeficiency virus (HIV) coinfection. However, data on the risk and predictors of VL relapse are scarce. Such information is vital to target medical follow-up and interventions to those at highest risk. Methods: We conducted a retrospective cohort study at a Médecins Sans Frontières-supported health center in northwest Ethiopia...
July 20, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Vinícius Silva Belo, Eliana Aparecida Gregório, Rafael Gonçalves Teixeira-Neto, Ana Cristina Vianna Mariano da Rocha Lima, Agnes Antônia Sampaio Pereira, Andreza Pain Marcelino, Gustavo Fontes Paz, Eduardo Sérgio da Silva
One of the key components of the Brazilian Program for the Control of Visceral Leishmaniasis (PCLV) is the euthanasia of Leishmania-infected canine reservoirs, the detection of which depends on a screening procedure involving a Dual Path Platform(®) (DPP) immunoassay and a confirmatory enzyme-linked immunosorbent assay (ELISA). The aims of the present study were to evaluate the reliability of these techniques in a region of recent transmission of canine VL, to follow up the seroconversion 3-4 months after the initial diagnosis of DPP reactive but ELISA indeterminate or non-reactive dogs, and to identify the species of Leishmania in circulation in the area...
October 1, 2017: Preventive Veterinary Medicine
Alejandro Javier Krolewiecki, Maria Cristina Almazan, Marcelo Quipildor, Marisa Juarez, Jose Fernando Gil, Marco Espinosa, Maria Canabire, Silvana Pamela Cajal
Leishmania (Viannia) braziliensis is the species most frequently implicated with cutaneous and mucosal leishmaniasis in the Americas; its diagnosis is based on the identification of amastigotes in lesions, which is limited by low parasite burden. Leishmanin Skin Test (LST) is a support tool for diagnosis, based on delayed type hypersensitivity responses to Leishmania antigens injected intradermally, used in endemic areas as a complement to diagnosis. A retrospective analysis of individuals evaluated for their first episode of tegumentary leishmaniasis at a reference center in Argentina during the period 2006-2015 was performed, with the goal of assessing its usefulness as a support tool in the diagnosis of leishmaniasis...
October 2017: PLoS Neglected Tropical Diseases
Mohammad Mahdi Parvizi, Farhad Handjani, Mahmoodreza Moein, Gholamreza Hatam, Majid Nimrouzi, Jafar Hassanzadeh, Nasrin Hamidizadeh, Hamid Reza Khorrami, Mohammad Mehdi Zarshenas
BACKGROUND: Cutaneous leishmaniasis is one of the highly prevalent endemic diseases in the Middle East and North Africa. Many treatment modalities have been recommended for this condition but success rates remain limited. Herbal remedies have also been used for treatment but evidence-based clinical trials with these products are sparse. In-vitro and in-vivo studies have shown the anti-leishmanial and curative effects of extract of fruits and leaves of Juniperus excelsa (J. excelsa). The aim of this study was to determine the efficacy of topical J...
October 2017: PLoS Neglected Tropical Diseases
Rita de Cássia Pontello Rampazzo, Manuela da Silva Solcà, Liliane Celestino Sales Santos, Lais de Novaes Pereira, José Carlos Oliveira Guedes, Patrícia Sampaio Tavares Veras, Deborah Bittencourt Mothé Fraga, Marco Aurélio Krieger, Alexandre Dias Tavares Costa
Canine visceral leishmaniasis (CVL) is a systemic disease caused by Leishmania infantum. A precise CVL diagnosis would allow for a faster and more specific treatment. Quantitative PCR (qPCR) is a sensitive and specific technique that can diagnose CVL and also monitor parasite load in the animal during the course of the infection or treatment. The aim of this study was to develop a ready-to-use (gelified and freezer-free) duplex qPCR for the identification of infected animals. We combined a new qPCR protocol that detects the canine 18S rRNA gene with an existing protocol for L...
November 15, 2017: Veterinary Parasitology
Gilsane Lino von Poser, Maria Helena Vendruscolo, Gustavo Machado Neves, Luciano Porto Kagami, Luiz Carlos Rodrigues, Maria Luisa Diehl, Simone Cristina Baggio Gnoatto, Sérgio Augusto de Loreto Bordignon, Pedro Roosevelt Torres Romão, Vera Lucia Eifler Lima
BACKGROUND: Leishmaniasis reaches millions of people around the world. The control of the disease is difficult due to the restricted access to the diagnosis and medication and the low adherence to the treatment. Thus, more efficient drugs are need and natural products are good alternatives. Iridoids, natural products with reported leishmanicidal activity, can be exploited for the development of anti-Leishmania drugs. The aim of this study was to isolate and to investigate the in vitro activity of iridoids against Leishmania amazonensis and to compare the activity in silico of these compounds with those reported as active against this parasite...
October 2, 2017: Current Drug Discovery Technologies
Otacilio C Moreira, Zaida E Yadon, Elisa Cupolillo
Cutaneous leishmaniasis (CL) is spread worldwide and is the most common manifestation of leishmaniasis. Diagnosis is performed by combining clinical and epidemiological features, and through the detection of Leishmania parasites (or DNA) in tissue specimens or trough parasite isolation in culture medium. Diagnosis of CL is challenging, reflecting the pleomorphic clinical manifestations of this disease. Skin lesions vary in severity, clinical appearance, and duration, and in some cases, they can be indistinguishable from lesions related to other diseases...
September 28, 2017: Acta Tropica
Faria Hossain, Prakash Ghosh, Md Anik Ashfaq Khan, Malcolm S Duthie, Aarthy C Vallur, Alessandro Picone, Randall F Howard, Steven G Reed, Dinesh Mondal
Sustained elimination of Visceral Leishmaniasis (VL) requires the reduction and control of parasite reservoirs to minimize the transmission of Leishmania donovani infection. A simple, reproducible and definitive diagnostic procedure is therefore indispensable for the early and accurate detection of parasites in VL, Relapsed VL (RVL) and Post Kala-azar Dermal Leishmaniasis (PKDL) patients, all of whom are potential reservoirs of Leishmania parasites. To overcome the limitations of current diagnostic approaches, a novel quantitative real-time polymerase chain reaction (qPCR) method based on Taqman chemistry was devised for the detection and quantification of L...
2017: PloS One
Franklin B Magalhães, Artur L Castro Neto, Marilia B Nascimento, Wagner J T Santos, Zulma M Medeiros, Adelino S Lima Neto, Dorcas L Costa, Carlos H N Costa, Washington L C Dos Santos, Lain C Pontes de Carvalho, Geraldo G S Oliveira, Osvaldo P de Melo Neto
Current strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library...
2017: PloS One
Mahbuba Khatun, S M Sabbir Alam, Abed Hussain Khan, M Anwar Hossain, Jalaluddin Ashraful Haq, Md Shariful Alam Jilani, Mohammad Tariqur Rahman, Muhammad Manjurul Karim
OBJECTIVE: To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. METHODS: Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions...
August 2017: Asian Pacific Journal of Tropical Medicine
Panagiotis Krepis, Adamantia Krepi, Ioanna Argyri, Anastasios Aggelis, Alexandra Soldatou, Maria Tsolia
BACKGROUND: Visceral leishmaniasis (VL) remains a public health issue in Greece. The aim of this study was to describe the clinical and epidemiological characteristics of pediatric VL in our region as well as to evaluate the laboratory findings and the diagnostic techniques that are applied. METHODS: We retrospectively reviewed the medical records of all children diagnosed with VL in an 11-year period at a tertiary public hospital in the region of Athens. Demographic features, clinical information and laboratory findings were accessed...
September 20, 2017: Pediatric Infectious Disease Journal
Valeria S García, Verónica D G Gonzalez, Luis Gugliotta, Alexis Burna, Ana Demonte, Diego G Arias, Matías S Cabeza, Sergio A Guerrero
Visceral leishmaniasis is a public health problem worldwide. The early diagnosis in dogs is crucial, since they are an epidemiologically relevant reservoir of the disease. The aim of a field study is to early identify the disease allowing rapid intervention to reduce its effects. We propose an immunoagglutination test as a visual in situ method for diagnosis of canine visceral leishmaniasis. Latex-protein complexes were sensitized by covalent coupling of a chimeric recombinant antigen of Leishmania spp. onto polystyrene latex with carboxyl functionality...
November 2017: Experimental Parasitology
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