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leishmania diagnosis

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https://www.readbyqxmd.com/read/29329617/dna-detection-of-trypanosoma-evansi-diagnostic-validity-of-a-new-assay-based-on-loop-mediated-isothermal-amplification-lamp
#1
Qunbo Tong, Rui Chen, Qingming Kong, Julie Goossens, Magdalena Radwanska, Di Lou, Jianzu Ding, Bin Zheng, Yixiu Fu, Tianping Wang, Magez Stefan, Shaohong Lu
Trypanosoma evansi (T. evansi) is the most widely spread pathogenic trypanosome in the world. The control of trypanosomiasis depends on accurate diagnosis and effective treatment. Focusing on the presence of T. evansi in Asia, we developed a detection assay based on tracing phosphate ions (Pi) generated during LAMP targeting the variant surface glycoprotein (VSG) gene of Rode Trypanozoon antigenic type 1.2 (RoTat 1.2 VSG). The diagnostic potential as well as the use of the assay as a test-of-cure method after berenil treatment, was assessed in mice at different time points of infection...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29324838/canine-visceral-leishmaniasis-diagnosis-and-management-of-the-reservoir-living-among-us
#2
REVIEW
Bruno L Travi, Anabela Cordeiro-da-Silva, Filipe Dantas-Torres, Guadalupe Miró
This article reviews essential topics of canine visceral leishmaniasis (CVL) due to Leishmania infantum infection. It focuses on the current serological and molecular diagnostic methods used in epidemiological research and veterinary clinics to diagnose CVL and includes new point-of-care (POC) tests under development. The efficacy of different treatment regimens on the clinical improvement and infectiousness of dogs is also addressed. In the last section, the review provides a critical appraisal of the effectiveness of different control measures that have been implemented to curb disease transmission...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29318992/cutaneous-leishmaniasis-in-pediatric-patients-in-a-single-tertiary-hospital-in-ankara
#3
Ayşe Kaman, Gönül Tanır, Zeynep Gökçe Gayretli Aydın, Özge Metin, Türkan Aydın Teke, Fatma Nur Öz, Mesut Mungan
OBJECTIVE: Leishmaniasis is an infectious disease that is caused by a protozoan parasite of the Leishmania genus and that occurs worldwide. Leishmaniasis is endemic in southeastern Turkey and the neighboring Middle Eastern countries. The purpose of this study was to describe the clinical characteristics of patients admitted to our hospital with a diagnosis of cutaneous leishmaniasis (CL). METHODS: A total of 16 CL patients [11 (69%) boys and five (31%) girls] were admitted between January 2014 and December 2015...
December 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29317878/cytochrome-b-and-molecular-typing-of-leishmania-spp-in-a-passive-sampling-of-suspected-patients-with-cutaneous-leishmaniasis-in-sistan-and-baluchestan-province-eastern-iran
#4
Gholamreza Motalleb, Hadi Mirahmadi, Ahmad Zare-Zadeh, Ahmad Mehravaran
Background: Despite the high prevalence and drug resistance of disease in Sistan and Baluchestan Province of Iran, the species of cutaneous leishmaniasis (CL) has not been identified. In the present study, cytochrome b (Cyt b) was used in Sistan and Baluchestan to find species of Leishmania in suspected patients of CL using PCR-RFLP and DNA sequencing. Methods: This study was conducted from Oct 2015 to Oct 2016. The samples were collected from the individuals clinically suspected to CL and referred to Iran Shahr, Chabahar, Khash, Zabol, Zahedan, Mirjaveh, and Nikshahr health centers...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29314762/opportunistic-infections-in-patients-with-idiopathic-inflammatory-myopathies
#5
Ada Redondo-Benito, Adrian Curran, Ana Villar-Gomez, Ernesto Trallero-Araguas, Andreu Fernández-Codina, Iago Pinal-Fernandez, Jose Ángel Rodrigo-Pendás, Albert Selva-O'Callaghan
AIM: To describe the prevalence, clinical characteristics and risk factors of opportunistic infection (OI) in a cohort of patients with inflammatory myopathies, and compare mortality rates between those with and without OIs. METHODS: In total, 204 patients from our myositis cohort were reviewed to identify patients who had experienced an OI during the period 1986-2014. The patients' clinical characteristics, treatments received, and outcomes were systematically recorded...
January 5, 2018: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29307127/a-case-of-canine-neurological-leishmaniasis
#6
Rosanna Zobba, Maria Antonietta Evangelisti, Maria Lucia Manunta, Alberto Alberti
In this study we describe a case of neurological leishmaniasis in a dog, reported in an endemic area, with signs of multifocal intracranial lesions involving the vestibular system and the cerebellum. Serum biochemistry revealed a decrease of albumin-globulin ratio caused by an increase of alfa2-, beta-, and gamma- globulin, while antibody titers were highly positive for Leishmania spp. Magnetic resonance images of the brain were consistent with a granulomatous in ammation/infection. Cerebrospinal uid revealed a marked mononuclear pleocytosis and was positive to the Pandy Test, as well as to a Leishmania spp...
December 29, 2017: Veterinaria Italiana
https://www.readbyqxmd.com/read/29280072/new-primers-for-the-detection-leishmania-species-by-multiplex-polymerase-chain-reaction
#7
Carolina Cella Conter, Maria Valdrinez Campana Lonardoni, Sandra Mara Alessi Aristides, Rosilene Fressatti Cardoso, Thaís Gomes Verzignassi Silveira
Leishmaniasis is caused by protozoa of the Leishmania genus, which is divided into subgenus Viannia and Leishmania. In humans, the course of infection largely depends on the host-parasite relationship and primarily of the infective species. The objective of the present study was to design specific primers to the identification of Leishmania species using multiplex PCR. Four primers were designed, based on the GenBank sequences of the kDNA minicircle, amplifying 127 bp for subgenus Viannia, 100 bp for L. amazonensis, and 60 bp for Leishmania donovani complex and L...
December 26, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29261762/-the-mosquitoes-that-destroy-your-face-social-impact-of-cutaneous-leishmaniasis-in-south-eastern-morocco-a-qualitative-study
#8
Issam Bennis, Loubna Belaid, Vincent De Brouwere, Hind Filali, Hamid Sahibi, Marleen Boelaert
OBJECTIVE: To document the psychosocial burden of Cutaneous Leishmaniasis (CL) in rural communities in Southeastern Morocco. METHOD: Between March and April 2015, we conducted qualitative research in communities exposed to Leishmania major or L. tropica in Errachidia and Tinghir provinces. Twenty-eight focus groups discussions (FGDs) were realized, with a stratification by gender and tradition of medicine (users of folk versus professional medicine). Data were analyzed using content analysis...
2017: PloS One
https://www.readbyqxmd.com/read/29221850/isolation-and-molecular-characterization-of-leishmania-infantum-in-urine-from-patients-with-visceral-leishmaniasis-in-brazil
#9
Manoel Sebastião da Costa Lima, Andressa Cristina Lopes Hartkopf, Rosianne A de Souza Tsujisaki, Elisa Teruya Oshiro, Julie Teresa Shapiro, Maria de Fatima Cepa Matos, Maria Elizabeth Cavalheiros Dorval
Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient...
December 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29214528/molecular-identification-of-leishmania-spp-isolates-causes-cutaneous-leishmaniasis-cl-in-sanliurfa-province-turkey-where-cl-is-highly-endemic
#10
Gulcan Gurses, Mehmet Ozaslan, Fadile Yıldız Zeyrek, Ibrahim H Kılıç, Nebiye Yentür Doni, I Didem Karagöz, Nermin Uluca
Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP...
December 7, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29190217/the-role-of-histopathology-and-immunohistochemistry-in-the-diagnosis-of-cutaneous-leishmaniasis-without-discernible-leishman-donovan-bodies
#11
David Dias-Polak, Yuval Geffen, Ofer Ben-Izhak, Reuven Bergman
BACKGROUND: Histopathology plays an important role in the diagnosis of cutaneous leishmaniasis (CL) but Leishman-Donovan (LD) bodies may not always be discernible. Recently, anti-CD1a antibody (Ab), clone MTB1, was found to decorate LD bodies immunohistochemically. OBJECTIVE: Can histopathology without discernible LD bodies be used to diagnose CL, and can immunohistochemistry using anti-CD1a Ab, clone MTB1, detect LD bodies in these cases. METHODS: Suspected CL lesions were studied histopathologically and immunohistochemically, and the patients' clinical files were reviewed...
December 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29187956/-leg-ulcer-revealing-cutaneous-leishmaniasis
#12
Elalouani Elmehdi, Bachar Zerkly
Leishmaniases are parasitic diseases occurring in endemic tropical and subtropical areas and caused by protozoa of the genus leishmania, transmitted by a diptera (sand fly). We here report a case of topical cutaneous leishmaniasis discovered in a 15-year old boy with painless ulcer on his left leg, who had been staying in South Africa. Clinical examination showed painless non-itchy ulcer, occurred 1 month before, on the antero-internal part of his left leg with crusts and scars caused by insect bites, all evolving in a context of patient's general health status, without mucosal or visceral lesions...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29173539/leishmania-infantum-specific-ifn-%C3%AE-production-in-stimulated-blood-from-dogs-with-clinical-leishmaniosis-at-diagnosis-and-during-treatment
#13
Pamela Martínez-Orellana, Daniel Marí-Martorell, Sara Montserrat-Sangrà, Laura Ordeix, Gad Baneth, Laia Solano-Gallego
There is limited data regarding Leishmania infantum specific T cell mediated immunity in naturally infected sick dogs at the time of diagnosis and during anti-Leishmania treatment. Our aim was to investigate the kinetics of L. infantum specific IFN-γ production in dogs with leishmaniosis at the time of diagnosis and during treatment and to correlate it with specific L. infantum antibodies, blood parasitemia and clinicopathological findings. Thirty-four dogs were diagnosed with leishmaniosis based on physical examination, routine laboratory tests and L...
December 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29166500/final-diagnosis-of-86-cases-included-in-differential-diagnosis-of-american-tegumentary-leishmaniasis-in-a-brazilian-sample-a-retrospective-cross-sectional-study
#14
Fernanda Tirelli, Sebastian Vernal, Ana Maria Roselino
BACKGROUND: Cutaneous leishmaniasis is distributed worldwide, including Brazil. Its several clinical forms need to be distinguished from other dermatoses. Clinical similarities and lack of a gold standard diagnostic tool make leishmaniasis-like lesions a challenging diagnosis. OBJECTIVES.: To report the final diagnosis of patients primarily suspected of having American tegumentary leishmaniasis (ATL). METHODS.: A retrospective cross-sectional study was conducted on the basis of medical records of 437 patients with clinical suspicion of ATL, registered in electronic hospital system between 1980 and 2013...
September 2017: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29165208/case-report-first-coinfection-report-of-mixed-leishmania-infantum-leishmania-major-and-human-immunodeficiency-virus-acquired-immune-deficiency-syndrome-report-of-a-case-of-disseminated-cutaneous-leishmaniasis-in-iran
#15
Alireza Badirzadeh, Mehdi Mohebali, Sadaf Sabzevari, Majid Ghafoori, Kourosh Arzamani, Mohammad Seyyedin, Seyed Ahmad Hashemi
Visceral leishmaniasis, a neglected tropical disease, is the third most common opportunistic disease in immunosuppressed patients, such as those affected by the human immunodeficiency virus (HIV)/acquired immune deficiency syndrome. Although the reports have been characterized as Leishmania/HIV coinfections, the occurrence of a mixed infection by two Leishmania species in HIV-positive patients is rare. Here, we present an atypical case of disseminated cutaneous leishmaniasis (DCL) in a 26-year-old HIV-positive man...
November 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29160649/-performance-of-the-katex-test-in-screening-and-diagnosis-for-visceral-leishmaniasis-in-a-reference-hospital
#16
C Fernández-Roldán, J Rodríguez-Grangér, R Javier Martínez, M A López-Ruz, J M Navarro-Marí, J Gutiérrez-Fernández
OBJECTIVE: Performing of diagnostic test simple using samples not invasive in the diagnosis of visceral leishmaniasis (VL) may be very beneficial, being necessary comparing to traditional methods. The objective of this study was to know the reliability of test KAtex in the urine of patients with suspicion of VL. METHODS: Retrospectively were reviewed the medical histories of patients with suspected of VL to which are performed the test between 2009 and 2015. For its analysis were selected the patients to which is them had made study of the parasite in bone marrow...
November 21, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29153900/clinicopathological-findings-in-sick-dogs-naturally-infected-with-leishmania-infantum-comparison-of-five-different-clinical-classification-systems
#17
A Meléndez-Lazo, L Ordeix, M Planellas, J Pastor, L Solano-Gallego
A wide spectrum of clinical and clinicopathological findings in dogs with canine leishmaniosis (CanL) due to Leishmania infantum exists. However, the majority of clinical descriptions have been published a long time ago and recent studies in Europe are almost lacking. In addition, clinical classification of sick dogs is not well-standardized, with different classification systems used by clinical and epidemiological studies, making comparison of studies a difficult task. The aims of the study were to describe the clinicopathological findings of dogs naturally infected with L...
November 10, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29153064/-comparison-of-polymerase-chain-reaction-using-kinetoplast-dna-specific-primers-and-other-parasitological-methods-in-the-diagnosis-of-clinical-samples-of-suspected-patients-with-cutaneous-leishmaniasis-in-%C3%A5-anl%C3%A4-urfa
#18
Fadile Yıldız Zeyrek, Seray Töz, Fehmi Yüksel, Nevin Turgay, Yusuf Özbel
Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are seen endemically in Turkey and CL caused by Leishmania tropica is an important public health problem in southeastern as well as other regions of Turkey. The diagnosis has been usually made by clinical view of lesion and/or parasitologically using lesion aspiration smears. Histological examination does not, always reveal the parasite in the skin biopsy, particularly in chronic lesions. Besides this, due to CL infections caused by different species in endemic areas, diagnostic methods enabling species identification are in great need...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29128045/recombinant-prohibitin-protein-of-leishmania-infantum-acts-as-a-vaccine-candidate-and-diagnostic-marker-against-visceral-leishmaniasis
#19
Daniel S Dias, Patrícia A F Ribeiro, Vívian T Martins, Daniela P Lage, Fernanda F Ramos, Anna L T Dias, Marcella R Rodrigues, Áquila S B Portela, Lourena E Costa, Rachel B Caligiorne, Bethina T Steiner, Miguel A Chávez-Fumagalli, Beatriz C S Salles, Thaís T O Santos, Julia A G Silveira, Danielle F Magalhães-Soares, Bruno M Roatt, Ricardo A Machado-de-Ávila, Mariana C Duarte, Daniel Menezes-Souza, Eduardo S Silva, Alexsandro S Galdino, Eduardo A F Coelho
Visceral leishmaniasis (VL) represents a serious public health problem, as Leishmania infantum is one of main disease causative agents in the Americas. In a previous immunoproteomic study, the prohibitin (PHB) protein was identified in L. infantum promastigote and amastigote extracts by antibodies in asymptomatic and symptomatic VL dog sera. This protein was found to be highly conserved between different Leishmania spp., but it presented a low identity with amino acid sequences of other organisms. The aim of the present study was to evaluate the cellular response induced by the recombinant PHB (rPHB) protein in BALB/c mice, as well as in PBMCs purified from untreated and treated VL patients, as well as to evaluate its protective efficacy against an infection by L...
November 9, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/29113597/antigenicity-immunogenicity-and-protective-efficacy-of-a-conserved-leishmania-hypothetical-protein-against-visceral-leishmaniasis
#20
Daniel S Dias, Vívian T Martins, Patrícia A F Ribeiro, Fernanda F Ramos, Daniela P Lage, Grasiele S V Tavares, Débora V C Mendonça, Miguel A Chávez-Fumagalli, Jamil S Oliveira, Eduardo S Silva, Dawidson A Gomes, Michele A Rodrigues, Mariana C Duarte, Alexsandro S Galdino, Daniel Menezes-Souza, Eduardo A F Coelho
In this study, a Leishmania hypothetical protein, LiHyS, was evaluated regarding its antigenicity, immunogenicity and protective efficacy against visceral leishmaniasis (VL). Regarding antigenicity, immunoblottings and an enzyme-linked immunosorbent assay using human and canine sera showed high sensitivity and specificity values for the recombinant protein (rLiHyS) in the diagnosis of VL. When evaluating the immunogenicity of LiHyS, which is possibly located in the parasite's flagellar pocket, proliferative assays using peripheral blood mononuclear cells from healthy subjects or VL patients showed a high proliferative index in both individuals, when compared to the results obtained using rA2 or unstimulated cultures...
November 8, 2017: Parasitology
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