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leishmania diagnosis

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https://www.readbyqxmd.com/read/28438522/detection-of-leishmania-infantum-dna-in-conjunctival-swabs-of-cats-by-quantitative-real-time-pcr
#1
Julia Cristina Benassi, Graziella U Benvenga, Helena Lage Ferreira, Vanessa F Pereira, Lara B Keid, Rodrigo Soares, Tricia Maria Ferreira de Sousa Oliveira
Although some studies have investigated the potential role of cats as a reservoir for Leishmania, their role in the epidemiology of visceral leishmaniasis (VL) is still poorly understood. Molecular diagnostic techniques are an important tool in VL diagnosis, and PCR shows high sensitivity and specificity for Leishmania spp. detection. Quantitative real-time PCR (qPCR) is a method that permits quantitative analysis of a large number of samples, resulting in more sensitive, accurate, and reproducible measurements of specific DNA present in the sample...
April 21, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28433944/evaluation-of-vaccinal-effectiveness-of-preparations-containing-membrane-antigens-of-leishmania-l-amazonensis-in-experimental-cutaneous-leishmaniasis-model
#2
João G Ribeiro, Amália S Ferreira, Sharon R A Macedo, Norton R D L P Rossi, Mayara C P da Silva, Rosane N M Guerra, Neuza B de Barros, Roberto Nicolete
American tegumentary leishmaniasis (ATL) is considered a neglected disease, for which an effective vaccine or an efficient diagnosis is not yet available and whose chemotherapeutic arsenal is threatened by the emergence of resistance by etiological agents such as Leishmania amazonensis. ATL is endemic in poor countries and has a high incidence in Brazil. Vaccines developed from native parasite fractions have led to the identification of defined antigenic subunits and the development of vaccine adjuvant technology...
April 20, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28427957/pcr-based-identification-of-trypanosoma-lewisi-and-trypanosoma-musculi-using-maxicircle-kinetoplast-dna
#3
Xiao-Kun Hong, Xuan Zhang, Octavio Alejandro Fusco, You-Gen Lan, Zhao-Rong Lun, De-Hua Lai
Trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. Its closely related species with global distribution, Trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. These trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. In this study, we have developed a rapid, sensitive and reliable PCR method for the diagnosis of T. lewisi and T. musculi. The T. lewisi-specific amplicons were not produced by other Trypanosoma, such as T...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28399053/short-course-regimens-of-liposomal-amphotericin-b-for-the-treatment-of-mediterranean-visceral-leishmaniasis-in-children-an-11-year-retrospective-study-at-a-tertiary-care-center
#4
Panagiotis Krepis, Ioanna Argyri, Adamantia Krepi, Areti Syrmou, Nikos Spyridis, Maria Tsolia
BACKGROUND: Visceral leishmaniasis (VL) remains an important public health problem in endemic regions. Current anti-leishmanial agents share several limitations including potentially serious side effects and the risk of clinical failure. OBJECTIVES: Aim of the present study was to examine the effectiveness and safety of short-course liposomal amphotericin B (L-AmB) regimens in the treatment of childhood VL in our area. METHODS: The cases of 43 VL patients (20 males, 23 females, mean age 4...
April 10, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28385540/performance-of-leishmania-braziliensis-enolase-protein-for-the-serodiagnosis-of-canine-and-human-visceral-leishmaniosis
#5
Mariana Costa Duarte, Daniela Pagliara Lage, Vívian Tamietti Martins, Lourena Emanuele Costa, Beatriz Cristina Silveira Salles, Ana Maria Ravena Severino Carvalho, Thaís Teodoro de Oliveira Santos, Daniel Silva Dias, Patrícia Aparecida Fernandes Ribeiro, Miguel Angel Chávez-Fumagalli, Ricardo Andrez Machado-de-Ávila, Bruno Mendes Roatt, Daniel Menezes-Souza, Danielle Ferreira de Magalhães-Soares, Eduardo Antonio Ferraz Coelho
In the present study, Leishmania braziliensis enolase was cloned and the recombinant protein (rEnolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (VL). For the canine VL diagnosis, this study examined serum samples of Leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from Leish-Tec(®)-vaccinated dogs and Trypanosoma cruzi or Ehrlichia canis experimentally infected animals. For the human VL diagnosis, this study analyzed serum samples from VL patients, from non-infected subjects living in endemic or non-endemic areas of leishmaniosis, as well as those from T...
March 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28382481/anion-exchange-through-band-3-protein-in-canine-leishmaniasis-at-different-stages-of-disease
#6
Rossana Morabito, Alessia Remigante, Mauro Cavallaro, Alessandro Taormina, Giuseppina La Spada, Angela Marino
Band 3 protein efficiency in mediating Cl(-)/HCO3(-) exchange through erythrocytes membrane is reduced by oxidative stress. The aim of the present study was to verify whether and how anion transport through band 3 protein may be useful in monitoring canine leishmaniasis (Leishmania infantum) development, a disease associated to membrane protein degradation and oxidative stress. To accomplish this aim, serological analysis to determine IFAT (immunofluorescence antibody test) titers against leishmaniasis has been performed and 1:160 and 1:540 titers, determined at diagnosis and after 6 months, were considered for experiments...
April 5, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28367456/synthetic-peptides-as-potential-antigens-for-cutaneous-leishmaniosis-diagnosis
#7
Juliana Seger Link, Silvana Maria Alban, Carlos Ricardo Soccol, Gilberto Vinicius Melo Pereira, Vanete Thomaz Soccol
This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28349614/progressive-histoplasmosis-with-hemophagocytic-lymphohistiocytosis-and-epithelioid-cell-granulomatosis-a-case-report-and-review-of-the-literature
#8
A B Schulze, B Heptner, T Kessler, B Baumgarten, V Stoica, M Mohr, R Wiewrodt, V Warneke, W Hartmann, J Wüllenweber, C Schülke, M Schäfers, D Wilmes, K Becker, L H Schmidt, A H Groll, W E Berdel
Histoplasmosis in central Europe is a rare fungal disease with diverse clinical presentations. Apart from acute pulmonary histoplasmosis and involvement of the central nervous system, the most serious clinical presentation is progressive disseminated histoplasmosis which is generally associated with severe immunodeficiency and in particular, advanced HIV-infection. Here we report on an immunocompetent female residing in a non-endemic area, presenting with progressive disseminated histoplasmosis after a remote travel history to Thailand and Costa Rica...
March 27, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28348785/retrospective-pcr-based-species-identification-of-leishmania-in-two-patients-with-visceral-leishmaniasis-in-serbia
#9
Zorica Dakić, Henrik Vedel Nielsen, Milorad Pavlović, Jasmina Poluga, Goran Stevanović, Lidija Lavadinović, Branko Milošević, Mijomir Pelemiš, Aleksandar Urošević, Snežana Jovanović, Christen Rune Stensvold
INTRODUCTION: Retrospective molecular identification of Leishmania parasites in two patients with visceral leishmaniasis (VL) previously treated in Serbia was carried out. DNA was isolated from unstained bone marrow smears (BMSs) kept for 11 and 8 years. Genus-specific real-time PCR was combined with conventional PCR and sequencing for detection and species identification. CASE PRESENTATION: In 2003, a 40-year-old Serbian male was admitted to the Clinical Centre of Serbia (CCS) with fever, sweating, fatigue and splenomegaly, which developed over a period of 7 weeks...
October 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28335752/development-of-a-rapid-loop-mediated-isothermal-amplification-assay-for-diagnosis-and-assessment-of-cure-of-leishmania-infection
#10
Sandeep Verma, Ruchi Singh, Vanila Sharma, Ram Avtar Bumb, Narendra Singh Negi, V Ramesh, Poonam Salotra
BACKGROUND: Leishmaniasis is a spectrum of diseases with great relevance to public health. Conventional diagnostic methods are time consuming, needing trained personnel. A robust, rapid and cost effective diagnostic test is warranted for on-time diagnosis and field application. METHODS: We have developed a loop mediated isothermal amplification (LAMP) assay with primers (n = 6) based on Leishmania donovani kDNA for detection of Leishmania infection, using a closed tube to prevent cross-contamination...
March 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28329481/cutaneous-leishmaniasis-mimicking-squamous-cell-carcinoma
#11
Tara Oetken, Bryan Hiscox, Ida Orengo, Theodore Rosen
A 41-year-old man from Cuero, Texas with a nonhealinglesion on his left cheek was referred to ourclinic for removal of a squamous cell carcinoma. Thepatient first noticed a "pimple" on his left cheek 3-4months prior to presentation. When the lesion beganto grow he presented to his primary care physician anda biopsy was taken, showing "atypical squamous cellproliferation." Mohs surgery was performed and thenodule was removed with no evidence of malignancyseen on histopathology. Upon review of the surgicalbiopsies by consulting pathologists, the diagnosis ofleishmaniasis was established and later confirmed bythe Center for Disease Control and Prevention (CDC)as Leishmania mexicana...
January 15, 2017: Dermatology Online Journal
https://www.readbyqxmd.com/read/28327803/serodiagnosis-of-visceral-and-cutaneous-leishmaniasis-in-human-and-canine-populations-living-in-indigenous-reserves-in-the-brazilian-amazon-region
#12
Julia Teresa Ribeiro de Lima, Solange Maria Gennari, Herbert Sousa Soares, Antonio Humberto Hamad Minervino, Antonio Francisco Malheiros, Fernando Silveira Marques, Márcia Dalastra Laurenti, Rosangela Zacarias Machado, Arlei Marcili, Marcelo Bahia Labruna, Rodrigo Martins Soares
INTRODUCTION: Leishmaniasis is endemic to the Northern, Northeastern, Central-Western, and Southeastern regions of Brazil. We aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the States of Mato Grosso and Tocantins using a serological survey conducted in May 2011. METHODS: Serum samples were collected from 470 humans and 327 dogs living in villages of the Urubu Branco and Tapirapé Karajá indigenous reserves...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28303124/identification-and-characterization-of-mirnas-in-response-to-leishmania-donovani-infection-delineation-of-their-roles-in-macrophage-dysfunction
#13
Neeraj Tiwari, Vinod Kumar, Mallikarjuna Rao Gedda, Ashish K Singh, Vijay K Singh, Sreenivas Gannavaram, Surya P Singh, Rakesh K Singh
The outcome of Leishmania infection depends on parasite abilities to evade host immune response and its survival in hostile environment of host macrophages. Despite a wealth of gained crucial information, parasite strategies by which it dampens host macrophage functions remain poorly understood. Micro RNAs (miRNAs) are evolutionarily conserved class of endogenous 22-nucleotide small non-coding RNA gene products, described to participate in the regulation of almost every cellular process investigated so far...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28301553/multiplex-pcr-as-a-tool-for-the-diagnosis-of-leishmania-spp-kdna-and-the-gapdh-housekeeping-gene-of-mammal-hosts
#14
Renata de Cássia-Pires, Myllena de Fátima Alheiros Dias de Melo, Raquel da Hora Barbosa, André Luiz Rodrigues Roque
BACKGROUND: The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain segment of a conserved region of the mammalian gapdh gene. METHODOLOGY: The proposed multiplex protocol was designed through in silico PCR. The annealing temperature, concentration of primer pairs, number of cycles, distinct polymerase enzymes and premix kit were defined to achieve an optimal reaction...
2017: PloS One
https://www.readbyqxmd.com/read/28294400/chronic-amastigote-negative-cutaneous-leishmaniasis-a-clinical-histopathologic-and-molecular-study-of-27-cases-with-emphasis-on-atypical-and-pseudolymphomatous-presentations
#15
Carlo Tomasini, Laura Moneghini, Maria Barbui Anna
BACKGROUND: Chronic amastigote-negative cutaneous leishmaniasis (CL) is a diagnostic challenge, as the parasite load may be low, or absent in biopsy tissue sections. METHODS: A series of consecutive biopsy specimens, taken from 130 patients with a diagnosis of granulomatous dermatitis of unknown etiology, were reviewed. PCR was carried out for Leishmania-specific DNA. RESULTS: A total of 27/130 samples were positive for Leishmania-specific DNA...
March 10, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28285598/diagnostic-performance-of-elisa-ifat-and-western-blot-for-the-detection-of-anti-leishmania-infantum-antibodies-in-cats-using-a-bayesian-analysis-without-a-gold-standard
#16
Maria Flaminia Persichetti, Laia Solano-Gallego, Angela Vullo, Marisa Masucci, Pierre Marty, Pascal Delaunay, Fabrizio Vitale, Maria Grazia Pennisi
BACKGROUND: Anti-Leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis. The immunofluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological tests more frequently used. The aim of the present study was to assess diagnostic performance of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian approach without a gold standard...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28285595/evaluation-of-four-molecular-methods-to-detect-leishmania-infection-in-dogs
#17
Andreia Albuquerque, Lenea Campino, Luís Cardoso, Sofia Cortes
BACKGROUND: Canine leishmaniasis, a zoonotic disease caused by Leishmania infantum vectored by phlebotomine sand flies, is considered a relevant veterinary and public health problem in various countries, namely in the Mediterranean basin and Brazil, where dogs are considered the main reservoir hosts. Not only diseased dogs but also those subclinically infected play a relevant role in the transmission of L. infantum to vectors; therefore, early diagnosis is essential, under both a clinical and an epidemiological perspective...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28285583/molecular-detection-of-vector-borne-pathogens-in-blood-and-splenic-samples-from-dogs-with-splenic-disease
#18
Rebeca Movilla, Laura Altet, Lorena Serrano, María-Dolores Tabar, Xavier Roura
BACKGROUND: The spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (VBP), which could have a fundamental role in the pathogenesis of splenic disease. This study aimed to evaluate certain VBP in samples from dogs with splenic lesions. METHODS: Seventy-seven EDTA-blood and 64 splenic tissue samples were collected from 78 dogs with splenic disease in a Mediterranean area. Babesia spp., Bartonella spp., Ehrlichia/Anaplasma spp...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28282232/an-atypical-case-of-leishmaniasis-associated-with-chronic-duodenitis-in-a-dog
#19
Ignacio Ayala, Luis Jesus Bernal, Juan Diego Garcia-Martinez, Miguel Angel Gomez, Jose Antonio Navarro, Antonio Bernabe
We describe an atypical case of duodenal leishmaniasis in a boxer dog presenting with chronic diarrhea and poor general condition. Antidiarrheic therapy was previously administered without success and inflammatory bowel disease localized to the small intestine was suspected, given the chronic clinical signs and by ruling out other known causes of gastrointestinal inflammation. Endoscopic biopsy of duodenum showed a moderate increase in lamina propria lymphocytes, plasma cells, and macrophages. Basophilic bodies were seen in the cytoplasm of numerous macrophages, suggestive of Leishmania spp, confirmed by immunostaining, and a diagnosis of granulomatous duodenitis associated to Leishmania infection was made...
March 2017: Journal of the American Animal Hospital Association
https://www.readbyqxmd.com/read/28273127/accuracy-of-chimeric-proteins-in-the-serological-diagnosis-of-chronic-chagas-disease-a-phase-ii-study
#20
Fred Luciano Neves Santos, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Wayner Vieira de Souza, Edimilson Domingos da Silva, Leonardo Foti, Marco Aurélio Krieger, Yara de Miranda Gomes
BACKGROUND: The performance of current serologic tests for diagnosing chronic Chagas disease (CD) is highly variable. The search for new diagnostic markers has been a constant challenge for improving accuracy and reducing the number of inconclusive results. METHODOLOGY/PRINCIPAL FINDINGS: Here, four chimeric proteins (IBMP-8.1 to -8.4) comprising immunodominant regions of different Trypanosoma cruzi antigens were tested by enzyme-linked immunosorbent assay. The proteins were used to detect specific anti-T...
March 2017: PLoS Neglected Tropical Diseases
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