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https://www.readbyqxmd.com/read/28526964/characterization-of-a-new-isolate-of-pepper-chlorotic-spot-virus-from-yunnan-province-china
#1
Kuanyu Zheng, Tsung-Chi Chen, Shyi-Dong Yeh, Md Siddiqur Rahman, Xiaoxia Su, Kuo Wu, Ting Li, Zhongkai Zhang, Jiahong Dong
A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80-120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively...
May 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28513179/consumption-of-watermelon-juice-enriched-in-l-citrulline-and-pomegranate-ellagitannins-enhanced-metabolism-during-physical-exercise
#2
Ascension Martinez-Sanchez, Fernando Alacid, Jacobo A Rubio-Arias, Bárbara Fernandez-Lobato, Domingo J Ramos-Campo, Encarna Aguayo
L-citrulline is a non-essential amino acid precursor of arginine and indirectly a precursor of nitric oxide (NO), which is a vasodilator and increases mitochondrial respiration. On the other hand, the antioxidant pomegranate ellagitannins are precursors of urolithin A, which has been associated with mitophagy and increased muscle function. To elucidate if a single dose of watermelon enrichment with these compounds have a positive effect after a high intensity exercise (8 sets of 8 repetitions of half squat exercise), a double-blind randomized crossover in vivo study was performed in healthy male subjects (n=19)...
May 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28507410/isolation-and-identification-of-fungi-associated-with-spoilt-fruits-vended-in-gwagwalada-market-abuja-nigeria
#3
Samuel Mailafia, God'spower Richard Okoh, Hamza Olatunde K Olabode, Ramatu Osanupin
AIM: Annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. This study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in Gwagwalada market, Abuja, and recommend appropriate control measure. MATERIALS AND METHODS: The study was conducted in Gwagwalada metropolis, Gwagwalada Area Council of the Federal Capital Territory, Abuja, Nigeria. A total of 100 spoilt fruits which include pawpaw (Carica papaya), orange (Citrus sinensis), tomato (Lycopersicon esculentum), pineapple (Ananas comosus), and watermelon (Citrullus vulgaris) were purchased and examined for the presence of fungal organisms using standard methods...
April 2017: Veterinary World
https://www.readbyqxmd.com/read/28502647/an-accurate-specific-sensitive-high-throughput-method-based-on-a-microsphere-immunoassay-for-multiplex-detection-of-three-viruses-and-bacterial-fruit-blotch-bacterium-in-cucurbits
#4
Ratthaphol Charlermroj, Manlika Makornwattana, Orawan Himananto, Channarong Seepiban, Sudtida Phuengwas, Nuchnard Warin, Oraprapai Gajanandana, Nitsara Karoonuthaisiri
To employ a microsphere immunoassay (MIA) to simultaneously detect multiple plant pathogens (potyviruses, Watermelon silver mottle virus, Melon yellow spot virus, and Acidovorax avenae subsp. citrulli) in actual plant samples, several factors need to be optimized and rigorously validated. Here, a simple extraction method using a single extraction buffer was successfully selected to detect the four pathogens in various cucurbit samples (cucumber, cantaloupe, melon, and watermelon). The extraction method and assay performance were validated with inoculated and field cucurbit samples...
May 11, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28490080/utilization-of-watermelon-pulp-for-lycopene-extraction-by-response-surface-methodology
#5
Davinder Pal Singh Oberoi, Dalbir Singh Sogi
Lycopene extraction was optimized from watermelon pulp using response surface methodology using independent variables:solvent/meal ratio (4:1-12:1v/w), number of extractions (1-5), temperature (20-60°C) and extraction time (4-20min). Watermelon pulp had 59.95mglycopene/100g on fresh weight basis. The experimental values of lycopene with selected combinations of independent variables were 8.20-59.17mg/100g on fresh weight basis. The second order model obtained revealed a coefficient of determination (R(2)) of 0...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28488082/genome-wide-identification-of-cucumber-green-mottle-mosaic-virus-responsive-micrornas-in-watermelon
#6
Yuyan Sun, Xiaowei Niu, Min Fan
Cucumber green mottle mosaic virus (CGMMV) is a damaging pathogen that attacks crop plants belonging to the family Cucurbitaceae. Little is known about the regulatory role of microRNAs (miRNAs) in response to CGMMV infection. To identify CGMMV-responsive miRNAs, two sRNA libraries from mock-inoculated and CGMMV-infected watermelon leaves were constructed and sequenced using Solexa sequencing technology. In total, 471 previously known and 1,809 novel miRNAs were obtained, of which 377 known and 246 novel miRNAs were found to be differentially expressed during CGMMV infection...
May 9, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28472825/lycopene-improves-insulin-sensitivity-through-inhibition-of-stat3-srebp-1c-mediated-lipid-accumulation-and-inflammation-in-mice-fed-a-high-fat-diet
#7
Zhaohui Zeng, Wang He, Zhen Jia, Shu Hao
In the past few years, metabolic disorders, such as type 2 diabetes and metabolic syndrome, has reached global prevalence. Lycopene is one of the major carotenoids in tomatoes, watermelons, red grapefruits, and guava. In the current study, using high fat diet (HFD)-fed mice, we investigated the effect of Lycopene on insulin resistance. We showed that diet containing Lycopene significantly prevented HFD-induced increase of fasting blood glucose and insulin level, glucose and insulin intolerance, and decrease of hepatic glycogen content...
May 4, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28463796/edible-seeds-from-cucurbitaceae-family-as-potential-functional-foods-immense-promises-few-concerns
#8
REVIEW
Seema Patel, Abdur Rauf
Cucurbitaceae family members such as pumpkin and watermelon have seeds that are discarded as the by-products of food processing. However, they have been discovered to contain a rich repertoire of nutrients such as proteins, unsaturated fatty acids, phenolic acids, carotenoids, tocopherol, phytosterol, squalene etc. Biological assays have proven the seed extracts to exert antioxidative, hypoglycemic, anticancer, antihypertensive, cardioprotective, antilipemic, gynoprotective, and anthelmintic properties. Further, the seeds do not contain any major anti-nutrients...
April 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28458683/full-chloroplast-genome-assembly-of-11-diverse-watermelon-accessions
#9
Chao Shi, Shuo Wang, Fei Zhao, Hua Peng, Chun-Lei Xiang
No abstract text is available yet for this article.
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28450024/extension-of-raw-watermelon-juice-shelf-life-up-to-58days-by-hyperbaric-storage
#10
Álvaro T Lemos, Ana C Ribeiro, Liliana G Fidalgo, Ivonne Delgadillo, Jorge A Saraiva
Hyperbaric storage (HS) of raw watermelon juice, at 50, 62.5 and 75MPa, at temperatures of 10, 15 and ≈25°C (room temperature, RT), was studied to evaluate shelf-life comparatively to refrigeration (RF, 4°C). Generally, RF caused an increase of microbial loads to values ≥6.0logCFU/mL after 7days of storage. Contrarily, HS at 62.5/75MPa (15°C) showed a reduction of initial loads, by at least 2.5logCFU/mL, up to 58days, while pH and colour values did not changed under these HS conditions. Additionally, the combination of a lower temperature with HS has beneficial effects to control microbial development, particularly for the lower pressure studied (50MPa/10°C)...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28445700/design-synthesis-of-novel-chitosan-derivatives-bearing-quaternary-phosphonium-salts-and-evaluation-of-antifungal-activity
#11
Wenqiang Tan, Jingjing Zhang, Fang Luan, Lijie Wei, Yuan Chen, Fang Dong, Qing Li, Zhanyong Guo
Two novel chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized, including tricyclohexylphosphonium acetyl chitosan chloride (TCPACSC) and triphenylphosphonium acetyl chitosan chloride (TPPACSC), and characterized by FTIR, (1)H NMR, and (13)C NMR spectra. The degree of substitution was also calculated by elemental analysis results. Their antifungal activities against Colletotrichum lagenarium, Watermelon fusarium, and Fusarium oxysporum were investigated in vitro using the radial growth assay, minimal inhibitory concentration, and minimum bactericidal concentration assay...
April 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28445588/the-relative-importance-of-pollinator-abundance-and-species-richness-for-the-temporal-variance-of-pollination-services
#12
Mark A Genung, Jeremy Fox, Neal M Williams, Claire Kremen, John Ascher, Jason Gibbs, Rachael Winfree
The relationship between biodiversity and the stability of ecosystem function is a fundamental question in community ecology, and hundreds of experiments have shown a positive relationship between species richness and the stability of ecosystem function. However, these experiments have rarely accounted for common ecological patterns, most notably skewed species abundance distributions and non-random extinction risks, making it difficult to know whether experimental results can be scaled up to larger, less manipulated systems...
April 26, 2017: Ecology
https://www.readbyqxmd.com/read/28436813/rf-heating-of-gold-cup-and-conductive-plastic-electrodes-during-simultaneous-eeg-and-mri
#13
Mukund Balasubramanian, William M Wells, John R Ives, Patrick Britz, Robert V Mulkern, Darren B Orbach
PURPOSE: To investigate the heating of EEG electrodes during magnetic resonance imaging (MRI) scans and to better understand the underlying physical mechanisms with a focus on the antenna effect. MATERIALS AND METHODS: Gold cup and conductive plastic electrodes were placed on small watermelons with fiberoptic probes used to measure electrode temperature changes during a variety of 1.5T and 3T MRI scans. A subset of these experiments was repeated on a healthy human volunteer...
2017: Neurodiagnostic Journal
https://www.readbyqxmd.com/read/28435133/de-novo-comparative-transcriptome-analysis-of-genes-involved-in-fruit-morphology-of-pumpkin-cultivars-with-extreme-size-difference-and-development-of-est-ssr-markers
#14
Aliki Xanthopoulou, Ioannis Ganopoulos, Fotis Psomopoulos, Maria Manioudaki, Theodoros Moysiadis, Aliki Kapazoglou, Maslin Osathanunkul, Sofia Michailidou, Apostolos Kalivas, Athanasios Tsaftaris, Irini Nianiou-Obeidat, Panagiotis Madesis
The genetic basis of fruit size and shape was investigated for the first time in Cucurbita species and genetic loci associated with fruit morphology have been identified. Although extensive genomic resources are available at present for tomato (Solanum lycopersicum), cucumber (Cucumis sativus), melon (Cucumis melo) and watermelon (Citrullus lanatus), genomic databases for Cucurbita species are limited. Recently, our group reported the generation of pumpkin (Cucurbita pepo) transcriptome databases from two contrasting cultivars with extreme fruit sizes...
April 20, 2017: Gene
https://www.readbyqxmd.com/read/28414630/infection-courts-in-watermelon-plants-leading-to-seed-infestation-by-fusarium-oxysporum-f-sp-niveum
#15
Aparna Petkar, Pingsheng Ji
Fusarium wilt incited by Fusarium oxysporum f. sp. niveum (FON) is a seed-transmitted disease that causes significant yield loss in watermelon production. The pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. However, information regarding infection courts of FON leading to infestation of watermelon seeds is limited. To determine how seeds in watermelon fruit can be infested by FON during the watermelon growing season, greenhouse and field experiments were conducted in 2014 and 2015 where watermelon flowers and immature fruit were inoculated with FON...
April 17, 2017: Phytopathology
https://www.readbyqxmd.com/read/28402027/simultaneous-determination-of-difenoconazole-trifloxystrobin-and-its-metabolite-trifloxystrobin-acid-residues-in-watermelon-under-field-conditions-by-gc-ms-ms
#16
Di Kang, Haizhen Zhang, Yuling Chen, Fei Wang, Lihong Shi, Deyu Hu, Kankan Zhang
An optimized quick, easy, cheap, effective, rugged and safe method for the simultaneous determination of difenoconazole, trifloxystrobin and its metabolite trifloxystrobin acid residues in watermelon and soil was developed and validated by gas chromatography with tandem mass spectrometry. The samples were extracted with acetonitrile (1% formic acid) and cleaned up by dispersive solid-phase extraction with octadecylsilane sorbent. The limit of quantification of the method was 0.01 mg/kg, and the limit of detection was 0...
April 12, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28398228/quantification-of-lycopene-%C3%AE-carotene-and-total-soluble-solids-in-intact-red-flesh-watermelon-citrullus-lanatus-using-on-line-near-infrared-spectroscopy
#17
Elena Tamburini, Stefania Costa, Irene Rugiero, Paola Pedrini, Maria Gabriella Marchetti
A great interest has recently been focused on lycopene and β-carotene, because of their antioxidant action in the organism. Red-flesh watermelon is one of the main sources of lycopene as the most abundant carotenoid. The use of near-infrared spectroscopy (NIRS) in post-harvesting has permitted us to rapidly quantify lycopene, β-carotene, and total soluble solids (TSS) on single intact fruits. Watermelons, harvested in 2013-2015, were submitted to near-infrared (NIR) radiation while being transported along a conveyor belt system, stationary and in movement, and at different positions on the belt...
April 11, 2017: Sensors
https://www.readbyqxmd.com/read/28393615/fractionation-amino-acid-profiles-antimicrobial-and-free-radical-scavenging-activities-of-citrullus-lanatus-seed-protein
#18
Priyanka Dash, Goutam Ghosh
In the present study, a modified Osborne fractionation method was followed to isolate albumin (Calb), globulin (Cglo), prolamin (Cpro) and glutelin (Cglu) successively from seeds of Citrullus lanatus (watermelon). This research work was undertaken to investigate the antimicrobial and antioxidant activities of isolated protein fractions of C. lanatus seed. Amino acid composition and molecular weight distribution were determined to establish their relationship with antimicrobial and antioxidant activity. Among all the fractions, Cpro was found to be most effective against A...
April 10, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28388893/genome-wide-identification-and-expression-analysis-of-cllax-clpin-and-clabcb-genes-families-in-citrullus-lanatus-under-various-abiotic-stresses-and-grafting
#19
Chenliang Yu, Wenqi Dong, Yihua Zhan, Zong-An Huang, Zhimiao Li, Il Seop Kim, Chenghao Zhang
BACKGROUND: Auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. Auxin is transported by three kinds of major protein families, including the AUXIN RESISTANT 1/LIKE AUX1 (AUX⁄LAX) influx carriers, the PIN-FORMED (PIN) efflux carriers and the ATP binding cassette B/P-glycoprotein/Multidrug-resistance (ABCB/MDR/PGP) efflux/condition carriers. The biological function of several auxin transporter genes has been well characterized in Arabidopsis thaliana...
April 7, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28381964/biological-control-of-bacterial-fruit-blotch-of-watermelon-pathogen-acidovorax-citrulli-with-rhizosphere-associated-bacteria
#20
Mahesh Adhikari, Dil Raj Yadav, Sang Woo Kim, Young Hyun Um, Hyun Seung Kim, Seong Chan Lee, Jeong Young Song, Hong Gi Kim, Youn Su Lee
Bacterial fruit blotch (BFB), which is caused by Acidovorax citrulli, is a serious threat to watermelon growers around the world. The present study was conducted to screen effective rhizobacterial isolates against 35 different A. citrulli isolates and determine their efficacy on BFB and growth parameters of watermelon. Two rhizobacterial isolates viz. Paenibacillus polymyxa (SN-22), Sinomonas atrocyanea (NSB-27) showed high inhibitory activity in the preliminary screening and were further evaluated for their effect on BFB and growth parameters of three different watermelon varieties under greenhouse conditions...
April 2017: Plant Pathology Journal
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