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https://www.readbyqxmd.com/read/28302791/genomic-characterization-of-three-melon-necrotic-spot-viruses-detected-in-human-stool-specimens
#1
Rachel Marine, Christina Castro, Laura Magaña, Terry Fei Fan Ng, Kshama Aswath, Nikail Collins, Geun Woo Park, Jan Vinjé, M Steven Oberste
The complete coding sequences of three melon necrotic spot viruses (MNSVs) were obtained from viral metagenomics of stool samples from patients with acute gastroenteritis. These genomes were most similar to Spanish strains sequenced in 2003 and a novel MNSV watermelon strain in 2014.
March 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28298921/exogenous-melatonin-confers-salt-stress-tolerance-to-watermelon-by-improving-photosynthesis-and-redox-homeostasis
#2
Hao Li, Jingjing Chang, Hejie Chen, Zhongyuan Wang, Xiurong Gu, Chunhua Wei, Yong Zhang, Jianxiang Ma, Jianqiang Yang, Xian Zhang
Melatonin, a pleiotropic signal molecule, has been shown to play important roles in the regulation of plant growth, development, and responses to environmental stresses. Since a few species have been investigated to unveil the effect of exogenous melatonin on salt stress, the underlying mechanism of melatonin-mediated salt stress tolerance in other plant species still remains largely unknown. In this study, the effects of melatonin on leaf photosynthesis and redox homeostasis in watermelon were examined under salt stress (300 mM NaCl) along with different doses of melatonin (50, 150, and 500 μM) pretreatment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28290292/novel-edible-oil-sources-microwave-heating-and-chemical-properties
#3
Seyed Mohammad Bagher Hashemi, Amin Mousavi Khaneghah, Mohamed Koubaa, Jaime Lopez-Cervantes, Seyed Hossein Asadi Yousefabad, Seyedeh Fatemeh Hosseini, Masoumeh Karimi, Azam Motazedian, Samira Asadifard
The aim of this work was to investigate the effect of various microwave heating times (1, 3, 5, 10, and 15min) on the chemical properties of novel edible oil sources, including Mashhadi melon (Cucumis melo var. Iranians cv. Mashhadi), Iranian watermelon (Citrullus lanatus cv. Fire Fon), pumpkin (Cucurbita pepo subsp. pepo var. Styriaca), and yellow apple (Malus domestica cv. Golden Delicious) seed oils. The evaluated parameters were peroxide value (PV), conjugated diene (CD) and triene (CT) values, carbonyl value (CV), p-anisidine value (AnV), oil stability index (OSI), radical scavenging activity (RSA), total tocopherols, total phenolics, as well as chlorophyll and carotenoid contents...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28260140/complete-nucleotide-sequences-of-m-and-l-rnas-from-a-new-pepper-infecting-tospovirus-pepper-chlorotic-spot-virus
#4
Kuo-Shiou Huang, Chia-Hsing Tai, Ying-Huey Cheng, Shu-Hui Lin, Tsung-Chi Chen, Fuh-Jyh Jan
Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330...
March 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28250580/genome-wide-identification-and-comparative-expression-analysis-of-lea-genes-in-watermelon-and-melon-genomes
#5
Yasemin Celik Altunoglu, Mehmet Cengiz Baloglu, Pinar Baloglu, Esra Nurten Yer, Sibel Kara
Late embryogenesis abundant (LEA) proteins are large and diverse group of polypeptides which were first identified during seed dehydration and then in vegetative plant tissues during different stress responses. Now, gene family members of LEA proteins have been detected in various organisms. However, there is no report for this protein family in watermelon and melon until this study. A total of 73 LEA genes from watermelon (ClLEA) and 61 LEA genes from melon (CmLEA) were identified in this comprehensive study...
January 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28238875/the-genomic-and-biological-characterization-of-citrullus-lanatus-cryptic-virus-infecting-watermelon-in-china
#6
Min Xin, Mengji Cao, Wenwen Liu, Yingdang Ren, Chuantao Lu, Xifeng Wang
A dsRNA virus was detected in the watermelon (Citrullus lanatus) samples collected from Kaifeng, Henan province, China through the use of next generation sequencing of small RNAs. The complete genome of this virus is comprised of dsRNA-1 (1603nt) and dsRNA-2 (1466nt), both of which are single open reading frames and potentially encode a 54.2kDa RNA-dependent RNA polymerase (RdRp) and a 45.9kDa coat protein (CP), respectively. The RdRp and CP share the highest amino acid identities 85.3% and 75.4% with a previously reported Israeli strain Citrullus lanatus cryptic virus (CiLCV), respectively...
February 24, 2017: Virus Research
https://www.readbyqxmd.com/read/28192137/a-strategic-approach-for-direct-recovery-and-stabilization-of-fusarium-sp-ict-sac1-cutinase-from-solid-state-fermented-broth-by-carrier-free-cross-linked-enzyme-aggregates
#7
Sandeep A Chaudhari, Rekha S Singhal
The major hurdles in commercial exploitation of cutinase (having both esterolytic and lipolytic activities) with potent industrial applications are its high production cost, operational instability and reusability. Although commercially available in immobilized form, its immobilization process (synthesis of support/carrier) makes it expensive. Herein we tried to address multiple issues of production cost, stability, and reusability, associated with cutinase. Waste watermelon rinds, an agroindustrial waste was considered as a cheap support for solid state fermentation (SSF) for cutinase production by newly isolated Fusarium sp...
February 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28188719/-systemic-reaction-after-performing-a-food-prick-to-prick-test-a-case-report
#8
Karen Estefanía Hernández-Moreno, Libia Susana Diez
BACKGROUND: Skin prick test is the most widely used test for the diagnosis of IgE-mediated conditions. Commercial extracts are used for its performance, but in the case of fruits and vegetables it is preferable using fresh food. Although both tests possess a good safety profile, hypersensitivity reactions have been recorded. CLINICAL CASE: Forty-seven-year old woman with a history of persistent allergic rhinitis, sensitized to the pollen of grasses, olive and salsola; she was referred to an allergology department due to anaphylaxis triggered by the consumption of avocado, cantaloupe, carrots and watermelon...
January 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/28182757/horticultural-systems-engineering-and-economic-evaluations-of-short-term-plant-storage-techniques-as-a-labor-management-tool-for-vegetable-grafting-nurseries
#9
Chieri Kubota, Chao Meng, Young-Jun Son, Myles Lewis, Hans Spalholz, Russell Tronstad
This transdisciplinary study has a three-fold systems approach in evaluating a horticultural technology: 1) horticultural evaluations, 2) economic and resource analyses, and 3) systems engineering analyses, using low temperature storage as an example technology. Vegetable grafting is a technique to produce value-added seedlings but requires labor intensive nursery operations. Low temperature storage of seedlings for a short period of time can reduce peak production, but has not been evaluated at the extent demonstrated in this paper...
2017: PloS One
https://www.readbyqxmd.com/read/28178932/variability-among-cucurbitaceae-species-melon-cucumber-and-watermelon-in-a-genomic-region-containing-a-cluster-of-nbs-lrr-genes
#10
Jordi Morata, Pere Puigdomènech
BACKGROUND: Cucurbitaceae species contain a significantly lower number of genes coding for proteins with similarity to plant resistance genes belonging to the NBS-LRR family than other plant species of similar genome size. A large proportion of these genes are organized in clusters that appear to be hotspots of variability. The genomes of the Cucurbitaceae species measured until now are intermediate in size (between 350 and 450 Mb) and they apparently have not undergone any genome duplications beside those at the origin of eudicots...
February 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28154429/grafting-and-paladin-pic-21-for-nematode-and-weed-management-in-vegetable-production
#11
Nancy Kokalis-Burelle, David M Butler, Jason C Hong, Michael G Bausher, Greg McCollum, Erin N Rosskopf
Two years of field trials conducted in a Meloidogyne incognita-infested field evaluated grafting and Paladin Pic-21 (dimethyl disulfide:chloropicrin [DMDS:Pic] 79:21) for root-knot nematode and weed control in tomato and melon. Tomato rootstocks evaluated were; 'TX301', 'Multifort', and 'Aloha'. 'Florida 47' was the scion and the nongrafted control. A double crop of melon was planted into existing beds following tomato harvest. Melon rootstocks, C. metulifer and 'Tetsukabuto', were evaluated with nongrafted 'Athena' in year 1...
December 2016: Journal of Nematology
https://www.readbyqxmd.com/read/28150427/effects-of-intermittent-co2-convection-under-far-infrared-radiation-on-vacuum-drying-of-pre-osmodehydrated-watermelon
#12
Rajat Chakraborty, Pijus Mondal
BACKGROUND: Watermelon, a tropical seasonal fruit with high nutrient content, requires preservation through drying due to its perishable nature. Nevertheless, drying of watermelon through conventional processes has negative impact either on the drying time or on the final product quality. In this work, osmotic dehydration (OD) of watermelon followed by far infrared radiation assisted vacuum-drying (FIRRAVD) was optimised to develop dehydrated watermelon with minimum moisture content. Significantly, during FIRRAVD, an attempt was made to further intensify the drying rate by forced convection through intermittent CO2 injection...
February 2, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28115090/novel-1-2-3-triazolium-functionalized-starch-derivatives-synthesis-characterization-and-evaluation-of-antifungal-property
#13
Wenqiang Tan, Qing Li, Fang Dong, Shuai Qiu, Jingjing Zhang, Zhanyong Guo
Four novel 1,2,3-triazolium-functionalized starch derivatives were synthesized by N-alkylating the precursor starch derivatives with 1,2,3-triazole with iodomethane based on cuprous-catalyzed azide-alkyne cycloaddition (CuAAC). The detailed structural characterization was investigated by means of FTIR, UV-vis, (1)H NMR, and (13)C NMR spectra. The antifungal activities of starch derivatives against Colletotrichum lagenarium, Fusarium oxysporum, and Watermelon fusarium, were then assayed by hypha measurement in vitro...
March 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28109844/colorimetric-detection-of-cucumber-green-mottle-mosaic-virus-using-unmodified-gold-nanoparticles-as-colorimetric-probes
#14
Lin Wang, Zhanmin Liu, Xueying Xia, Cuiyun Yang, Junyi Huang, Sibao Wan
Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction...
May 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28054163/genetic-variation-and-population-structure-of-cucumber-green-mottle-mosaic-virus
#15
Li-Xia Rao, Yushuang Guo, Li-Li Zhang, Xue-Ping Zhou, Jian Hong, Jian-Xiang Wu
Cucumber green mottle mosaic virus (CGMMV) is a single-stranded, positive sense RNA virus infecting cucurbitaceous plants. In recent years, CGMMV has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in China, causing serious losses to their production. In this study, we surveyed CGMMV infection in various cucurbitaceous crops grown in Zhejiang Province and in several seed lots purchased from local stores with the dot enzyme-linked immunosorbent assay (dot-ELISA), using a CGMMV specific monoclonal antibody...
January 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28049426/comparative-transcriptome-analysis-of-two-contrasting-watermelon-genotypes-during-fruit-development-and-ripening
#16
Qianglong Zhu, Peng Gao, Shi Liu, Zicheng Zhu, Sikandar Amanullah, Angela R Davis, Feishi Luan
BACKGROUND: Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process. RESULTS: In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177)...
January 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28040679/morphological-observation-rna-seq-quantification-and-expression-profiling-novel-insight-into-grafting-responsive-carotenoid-biosynthesis-in-watermelon-grafted-onto-pumpkin-rootstock
#17
Guang Liu, Xingping Yang, Jinhua Xu, Man Zhang, Qian Hou, Lingli Zhu, Ying Huang, Aisheng Xiong
Watermelon is an important and economical horticultural crop in China, where ~20% of the plants are grafted. The development of grafted watermelon fruit involves a diverse range of gene interactions that results in dynamic changes in fruit. However, the molecular mechanisms underlying grafting-induced fruit quality change are unclear. In the present study, we measured the lycopene content by high-performance liquid chromatography and used RNA-Seq (quantification) to perform a genome-wide transcript analysis of fruits from watermelon grafted onto pumpkin rootstock (pumpkin-grafted watermelon, PGW), self-grafted watermelon (SGW), and non-grafted watermelon (NGW)...
December 30, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28028039/draft-genome-sequence-of-bitter-gourd-momordica-charantia-a-vegetable-and-medicinal-plant-in-tropical-and-subtropical-regions
#18
Naoya Urasaki, Hiroki Takagi, Satoshi Natsume, Aiko Uemura, Naoki Taniai, Norimichi Miyagi, Mai Fukushima, Shouta Suzuki, Kazuhiko Tarora, Moritoshi Tamaki, Moriaki Sakamoto, Ryohei Terauchi, Hideo Matsumura
Bitter gourd (Momordica charantia) is an important vegetable and medicinal plant in tropical and subtropical regions globally. In this study, the draft genome sequence of a monoecious bitter gourd inbred line, OHB3-1, was analyzed. Through Illumina sequencing and de novo assembly, scaffolds of 285.5 Mb in length were generated, corresponding to ∼84% of the estimated genome size of bitter gourd (339 Mb). In this draft genome sequence, 45,859 protein-coding gene loci were identified, and transposable elements accounted for 15...
December 27, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/27995308/efficient-crispr-cas9-based-gene-knockout-in-watermelon
#19
Shouwei Tian, Linjian Jiang, Qiang Gao, Jie Zhang, Mei Zong, Haiying Zhang, Yi Ren, Shaogui Guo, Guoyi Gong, Fan Liu, Yong Xu
CRISPR/Cas9 system can precisely edit genomic sequence and effectively create knockout mutations in T0 generation watermelon plants. Genome editing offers great advantage to reveal gene function and generate agronomically important mutations to crops. Recently, RNA-guided genome editing system using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been applied to several plant species, achieving successful targeted mutagenesis. Here, we report the genome of watermelon, an important fruit crop, can also be precisely edited by CRISPR/Cas9 system...
March 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/27987923/design-synthesis-of-novel-starch-derivative-bearing-1-2-3-triazolium-and-pyridinium-and-evaluation-of-its-antifungal-activity
#20
Wenqiang Tan, Qing Li, Zhenpeng Gao, Shuai Qiu, Fang Dong, Zhanyong Guo
Based on cuprous-catalyzed azide-alkyne cycloaddition (CuAAC), starch derivative bearing 1,2,3-triazole and pyridine (II) was prepared and subsequently followed by alkylation with iodomethane to synthesize starch derivative bearing 1,2,3-triazolium and pyridinium (III). The antifungal activities of starch derivatives against Colletotrichum lagenarium, Watermelon fusarium, and Phomopsis asparagi, were then assayed by hypha measurement in vitro. Apparently, starch derivatives showed enhanced antifungal activity against three fungi at the tested concentrations compared with starch...
February 10, 2017: Carbohydrate Polymers
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