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https://www.readbyqxmd.com/read/28192137/a-strategic-approach-for-direct-recovery-and-stabilization-of-fusarium-sp-ict-sac1-cutinase-from-solid-state-fermented-broth-by-carrier-free-cross-linked-enzyme-aggregates
#1
Sandeep A Chaudhari, Rekha S Singhal
The major hurdles in commercial exploitation of cutinase (having both esterolutic and lipolytic activities) with potent industrial applications are its high production cost, operational instability and reusability. Although commercially available in immobilized form, its immobilization process (synthesis of support/carrier) makes it expensive. Herein we tried to address multiple issues of production cost, stability, and reusability, associated with cutinase. Waste watermelon rinds, an agroindustrial waste was considered as a cheap support for solid state fermentation (SSF) for cutinase production by newly isolated Fusarium sp...
February 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28188719/-systemic-reaction-after-performing-a-food-prick-to-prick-test-a-case-report
#2
Karen Estefanía Hernández-Moreno, Libia Susana Diez
BACKGROUND: Skin prick test is the most widely used test for the diagnosis of IgE-mediated conditions. Commercial extracts are used for its performance, but in the case of fruits and vegetables it is preferable using fresh food. Although both tests possess a good safety profile, hypersensitivity reactions have been recorded. CLINICAL CASE: Forty-seven-year old woman with a history of persistent allergic rhinitis, sensitized to the pollen of grasses, olive and salsola; she was referred to an allergology department due to anaphylaxis triggered by the consumption of avocado, cantaloupe, carrots and watermelon...
January 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/28182757/horticultural-systems-engineering-and-economic-evaluations-of-short-term-plant-storage-techniques-as-a-labor-management-tool-for-vegetable-grafting-nurseries
#3
Chieri Kubota, Chao Meng, Young-Jun Son, Myles Lewis, Hans Spalholz, Russell Tronstad
This transdisciplinary study has a three-fold systems approach in evaluating a horticultural technology: 1) horticultural evaluations, 2) economic and resource analyses, and 3) systems engineering analyses, using low temperature storage as an example technology. Vegetable grafting is a technique to produce value-added seedlings but requires labor intensive nursery operations. Low temperature storage of seedlings for a short period of time can reduce peak production, but has not been evaluated at the extent demonstrated in this paper...
2017: PloS One
https://www.readbyqxmd.com/read/28178932/variability-among-cucurbitaceae-species-melon-cucumber-and-watermelon-in-a-genomic-region-containing-a-cluster-of-nbs-lrr-genes
#4
Jordi Morata, Pere Puigdomènech
BACKGROUND: Cucurbitaceae species contain a significantly lower number of genes coding for proteins with similarity to plant resistance genes belonging to the NBS-LRR family than other plant species of similar genome size. A large proportion of these genes are organized in clusters that appear to be hotspots of variability. The genomes of the Cucurbitaceae species measured until now are intermediate in size (between 350 and 450 Mb) and they apparently have not undergone any genome duplications beside those at the origin of eudicots...
February 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28154429/grafting-and-paladin-pic-21-for-nematode-and-weed-management-in-vegetable-production
#5
Nancy Kokalis-Burelle, David M Butler, Jason C Hong, Michael G Bausher, Greg McCollum, Erin N Rosskopf
Two years of field trials conducted in a Meloidogyne incognita-infested field evaluated grafting and Paladin Pic-21 (dimethyl disulfide:chloropicrin [DMDS:Pic] 79:21) for root-knot nematode and weed control in tomato and melon. Tomato rootstocks evaluated were; 'TX301', 'Multifort', and 'Aloha'. 'Florida 47' was the scion and the nongrafted control. A double crop of melon was planted into existing beds following tomato harvest. Melon rootstocks, C. metulifer and 'Tetsukabuto', were evaluated with nongrafted 'Athena' in year 1...
December 2016: Journal of Nematology
https://www.readbyqxmd.com/read/28150427/effects-of-intermittent-co2-convection-under-far-infrared-radiation-on-vacuum-drying-of-pre-osmodehydrated-watermelon
#6
Rajat Chakraborty, Pijus Mondal
BACKGROUND: Watermelon, a tropical seasonal fruit with high nutrient content, requires preservation through drying due to its perishable nature. Nevertheless, drying of watermelon through conventional processes has negative impact either on the drying time or on the final product quality. In this work, osmotic dehydration (OD) of watermelon followed by far infrared radiation assisted vacuum-drying (FIRRAVD) was optimised to develop dehydrated watermelon with minimum moisture content. Significantly, during FIRRAVD, an attempt was made to further intensify the drying rate by forced convection through intermittent CO2 injection...
February 2, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28115090/novel-1-2-3-triazolium-functionalized-starch-derivatives-synthesis-characterization-and-evaluation-of-antifungal-property
#7
Wenqiang Tan, Qing Li, Fang Dong, Shuai Qiu, Jingjing Zhang, Zhanyong Guo
Four novel 1,2,3-triazolium-functionalized starch derivatives were synthesized by N-alkylating the precursor starch derivatives with 1,2,3-triazole with iodomethane based on cuprous-catalyzed azide-alkyne cycloaddition (CuAAC). The detailed structural characterization was investigated by means of FTIR, UV-vis, (1)H NMR, and (13)C NMR spectra. The antifungal activities of starch derivatives against Colletotrichum lagenarium, Fusarium oxysporum, and Watermelon fusarium, were then assayed by hypha measurement in vitro...
March 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28109844/colorimetric-detection-of-cucumber-green-mottle-mosaic-virus-using-unmodified-gold-nanoparticles-as-colorimetric-probes
#8
Lin Wang, Zhanmin Liu, Xueying Xia, Cuiyun Yang, Junyi Huang, Sibao Wan
Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction...
January 18, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28054163/genetic-variation-and-population-structure-of-cucumber-green-mottle-mosaic-virus
#9
Li-Xia Rao, Yushuang Guo, Li-Li Zhang, Xue-Ping Zhou, Jian Hong, Jian-Xiang Wu
Cucumber green mottle mosaic virus (CGMMV) is a single-stranded, positive sense RNA virus infecting cucurbitaceous plants. In recent years, CGMMV has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in China, causing serious losses to their production. In this study, we surveyed CGMMV infection in various cucurbitaceous crops grown in Zhejiang Province and in several seed lots purchased from local stores with the dot enzyme-linked immunosorbent assay (dot-ELISA), using a CGMMV specific monoclonal antibody...
January 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28049426/comparative-transcriptome-analysis-of-two-contrasting-watermelon-genotypes-during-fruit-development-and-ripening
#10
Qianglong Zhu, Peng Gao, Shi Liu, Zicheng Zhu, Sikandar Amanullah, Angela R Davis, Feishi Luan
BACKGROUND: Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process. RESULTS: In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177)...
January 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28040679/morphological-observation-rna-seq-quantification-and-expression-profiling-novel-insight-into-grafting-responsive-carotenoid-biosynthesis-in-watermelon-grafted-onto-pumpkin-rootstock
#11
Guang Liu, Xingping Yang, Jinhua Xu, Man Zhang, Qian Hou, Lingli Zhu, Ying Huang, Aisheng Xiong
Watermelon is an important and economical horticultural crop in China, where ~20% of the plants are grafted. The development of grafted watermelon fruit involves a diverse range of gene interactions that results in dynamic changes in fruit. However, the molecular mechanisms underlying grafting-induced fruit quality change are unclear. In the present study, we measured the lycopene content by high-performance liquid chromatography and used RNA-Seq (quantification) to perform a genome-wide transcript analysis of fruits from watermelon grafted onto pumpkin rootstock (pumpkin-grafted watermelon, PGW), self-grafted watermelon (SGW), and non-grafted watermelon (NGW)...
December 30, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28028039/draft-genome-sequence-of-bitter-gourd-momordica-charantia-a-vegetable-and-medicinal-plant-in-tropical-and-subtropical-regions
#12
Naoya Urasaki, Hiroki Takagi, Satoshi Natsume, Aiko Uemura, Naoki Taniai, Norimichi Miyagi, Mai Fukushima, Shouta Suzuki, Kazuhiko Tarora, Moritoshi Tamaki, Moriaki Sakamoto, Ryohei Terauchi, Hideo Matsumura
Bitter gourd (Momordica charantia) is an important vegetable and medicinal plant in tropical and subtropical regions globally. In this study, the draft genome sequence of a monoecious bitter gourd inbred line, OHB3-1, was analyzed. Through Illumina sequencing and de novo assembly, scaffolds of 285.5 Mb in length were generated, corresponding to ∼84% of the estimated genome size of bitter gourd (339 Mb). In this draft genome sequence, 45,859 protein-coding gene loci were identified, and transposable elements accounted for 15...
December 27, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/27995308/efficient-crispr-cas9-based-gene-knockout-in-watermelon
#13
Shouwei Tian, Linjian Jiang, Qiang Gao, Jie Zhang, Mei Zong, Haiying Zhang, Yi Ren, Shaogui Guo, Guoyi Gong, Fan Liu, Yong Xu
CRISPR/Cas9 system can precisely edit genomic sequence and effectively create knockout mutations in T0 generation watermelon plants. Genome editing offers great advantage to reveal gene function and generate agronomically important mutations to crops. Recently, RNA-guided genome editing system using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been applied to several plant species, achieving successful targeted mutagenesis. Here, we report the genome of watermelon, an important fruit crop, can also be precisely edited by CRISPR/Cas9 system...
December 19, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27987923/design-synthesis-of-novel-starch-derivative-bearing-1-2-3-triazolium-and-pyridinium-and-evaluation-of-its-antifungal-activity
#14
Wenqiang Tan, Qing Li, Zhenpeng Gao, Shuai Qiu, Fang Dong, Zhanyong Guo
Based on cuprous-catalyzed azide-alkyne cycloaddition (CuAAC), starch derivative bearing 1,2,3-triazole and pyridine (II) was prepared and subsequently followed by alkylation with iodomethane to synthesize starch derivative bearing 1,2,3-triazolium and pyridinium (III). The antifungal activities of starch derivatives against Colletotrichum lagenarium, Watermelon fusarium, and Phomopsis asparagi, were then assayed by hypha measurement in vitro. Apparently, starch derivatives showed enhanced antifungal activity against three fungi at the tested concentrations compared with starch...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27940267/nitric-oxide-protects-carbon-assimilation-process-of-watermelon-from-boron-induced-oxidative-injury
#15
Mohamed Farag, Ullah Najeeb, Jinghua Yang, Zhongyuan Hu, Zhang Ming Fang
Nitric oxide (NO) mediates plant response to a variety of abiotic stresses; however, limited information is available on its effect on boron (B)-stressed watermelon plants. The present study investigates the mechanism through which NO protects watermelon seedlings from B deficiency and toxicity stresses. Five days old watermelon seedlings were exposed to B (0, 0.5 and 10 mg L(-1)) alone or with 75 μmole of NO donor sodium nitroprusside (SNP) for 30 days. Both low and high B concentrations in the media altered nutrient accumulation and impaired various physiological processes of watermelon seedlings, leading to a significant reduction in biomass production...
November 30, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27919254/anti-inflammatory-and-in-vitro-antibacterial-activities-of-traditional-chinese-medicine-formula-qingdaisan
#16
Xinghua Zhao, Xin He, Xiuhui Zhong
BACKGROUND: Qingdaisan (Formulated Indigo powder, QDS) are widely used for treatment of aphtha, sore throat and bleeding gums in China. The aim of the study is to evaluate the anti-inflammatory, antibacterial and dental ulcer therapeutic effects of QDS. METHODS: Dimethylbenzene-induced ear edema test and cotton pellet-induced granuloma test were used to evaluate anti-inflammatory activities of QDS on acute and chronic inflammatory. The healing time and local pathologic changes were used to assess the therapeutic effects of QDS on dental ulcer...
December 5, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27913980/complete-nucleotide-sequences-and-construction-of-full-length-infectious-cdna-clones-of-cucumber-green-mottle-mosaic-virus-cgmmv-in-a-versatile-newly-developed-binary-vector-including-both-35s-and-t7-promoters
#17
Chan-Hwan Park, Hye-Kyoung Ju, Jae-Yeong Han, Jong-Seo Park, Ik-Hyun Kim, Eun-Young Seo, Jung-Kyu Kim, John Hammond, Hyoun-Sub Lim
Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields and generated full-length infectious cDNA clones. The full-length cDNAs were cloned into newly constructed binary vector pJY, which includes both the 35S and T7 promoters for versatile usage (agroinfiltration and in vitro RNA transcription) and a modified hepatitis delta virus ribozyme sequence to precisely cleave RNA transcripts at the 3' end of the tobamovirus genome...
December 2, 2016: Virus Genes
https://www.readbyqxmd.com/read/27897608/modulating-oxidative-stress-and-inflammation-in-elders-the-moxie-study
#18
Amy Cameron Ellis, Tanja Dudenbostel, Julie L Locher, Kristi Crowe-White
Cardiovascular disease (CVD) is the leading cause of death among women in the United States. Endothelial dysfunction and arterial stiffness increase with advancing age and are early predictors of future CVD outcomes. We designed the Modulating Oxidative Stress and Inflammation in Elders (MOXIE) study to examine the effects of 100% watermelon juice as a "food-first" intervention to reduce CVD risk among African American (AA) and European American (EA) women aged 55-69 years. Vascular dysfunction is more pronounced in AA compared to EA women due in part to lower nitric oxide bioavailability caused by higher oxidative stress...
October 2016: Journal of Nutrition in Gerontology and Geriatrics
https://www.readbyqxmd.com/read/27892922/convergence-and-divergence-of-bitterness-biosynthesis-and-regulation-in-cucurbitaceae
#19
Yuan Zhou, Yongshuo Ma, Jianguo Zeng, Lixin Duan, Xiaofeng Xue, Huaisong Wang, Tao Lin, Zhiqiang Liu, Kewu Zeng, Yang Zhong, Shu Zhang, Qun Hu, Min Liu, Huimin Zhang, James Reed, Tessa Moses, Xinyan Liu, Peng Huang, Zhixing Qing, Xiubin Liu, Pengfei Tu, Hanhui Kuang, Zhonghua Zhang, Anne Osbourn, Dae-Kyun Ro, Yi Shang, Sanwen Huang
Differentiation of secondary metabolite profiles in closely related plant species provides clues for unravelling biosynthetic pathways and regulatory circuits, an area that is still underinvestigated. Cucurbitacins, a group of bitter and highly oxygenated tetracyclic triterpenes, are mainly produced by the plant family Cucurbitaceae. These compounds have similar structures, but differ in their antitumour activities and ecophysiological roles. By comparative analyses of the genomes of cucumber, melon and watermelon, we uncovered conserved syntenic loci encoding metabolic genes for distinct cucurbitacins...
November 28, 2016: Nature Plants
https://www.readbyqxmd.com/read/27882891/a-comparative-mass-spectrometric-study-of-fatty-acids-and-metals-in-some-seed-extracts
#20
Sonia Niculina Suvar, R Bleiziffer, P Podea, A Iordache, C Voica, R Zgavarogea, M Culea
A major cause of cardiovascular diseases and cancer is diet content, so the optimization of micronutrients in food is very important. Omega-3 fatty acids supplementation for patients had beneficial effects on subjective global assessment score and metabolic profiles. Fatty acids content and the metal ions in different seeds (e.g. linseed, poppy, grape, hemp, nuts, pumpkin, sesame, watermelon, chia) recommended as food supplements, purchased on the Romanian market, were compared. Gas chromatography coupled to mass spectrometry (GC-MS) was used as an excellent technique for fatty acids identification and quantitation, and inductively coupled plasma mass spectrometer (ICP-MS) for analytical measurements of metals...
2016: European Journal of Mass Spectrometry
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