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Kaiying Chen, Xingang Liu, Xiaohu Wu, Fengshou Dong, Jun Xu, Yongquan Zheng
A new analytical method using the quick, easy, cheap, effective, rugged and safe (QuEChERS) procedure for simultaneous determination of afidopyropen and its metabolite M440I007 residues in tomato, watermelon, pepper, cucumber, pear, grape, cabbage and soil samples was developed using ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The target compound was determined in less than 5.0 min using an electrospray ionisation source in positive mode (ESI+). The limit of quantification was 1 μg kg-1 in different matrices...
January 17, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Xiaozhen Yang, Hao Li, Yongchao Yang, Yongqi Wang, Yanling Mo, Ruimin Zhang, Yong Zhang, Jianxiang Ma, Chunhua Wei, Xian Zhang
Despite identification of WRKY family genes in numerous plant species, a little is known about WRKY genes in watermelon, one of the most economically important fruit crops around the world. Here, we identified a total of 63 putative WRKY genes in watermelon and classified them into three major groups (I-III) and five subgroups (IIa-IIe) in group II. The structure analysis indicated that ClWRKYs with different WRKY domains or motifs may play different roles by regulating respective target genes. The expressions of ClWRKYs in different tissues indicate that they are involved in various tissue growth and development...
2018: PloS One
Xiaohua He, Fuzhen Guo, Bin Liu
Simplifying sample processing, shortening the sample preparation time, and adjusting procedures to suitable for new health and safety regulations, these issues are the current challenges which electron microscopic examinations need to face. In order to resolve these problems, new plant tissue sample processing protocols for transmission electron microscopy should be developed. In the present study, we chose the LR-White resin-assisted processing protocol for the ultrastructural observation of different types of plant tissues...
January 15, 2018: Journal of Microscopy
Lei Gao, Shengjie Zhao, Xuqiang Lu, Nan He, Hongju Zhu, Junling Dou, Wenge Liu
Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus) fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening...
2018: PloS One
Samira Sedehi, Hadi Tabani, Saeed Nojavan
In this work, polypropylene hollow fiber was replaced by agarose gel in conventional electro membrane extraction (EME) to develop a novel approach. The proposed EME method was then employed to extract two amino acids (tyrosine and phenylalanine) as model polar analytes, followed by HPLC-UV. The method showed acceptable results under optimized conditions. This green methodology outperformed conventional EME, and required neither organic solvents nor carriers. The effective parameters such as the pH values of the acceptor and the donor solutions, the thickness and pH of the gel, the extraction voltage, the stirring rate, and the extraction time were optimized...
March 1, 2018: Talanta
Houston Wilson, Jessica S Wong, Robbin W Thorp, Albie F Miles, Kent M Daane, Miguel A Altieri
Agricultural expansion and intensification negatively affect pollinator populations and has led to reductions in pollination services across multiple cropping systems. As a result, growers and researchers have utilized the restoration of local and landscape habitat diversity to support pollinators, and wild bees in particular. Although a majority of studies to date have focussed on effects in pollinator-dependent crops such as almond, tomato, sunflower, and watermelon, supporting wild bees in self-pollinated crops, such as grapes, can contribute to broader conservation goals as well as provide other indirect benefits to growers...
December 30, 2017: Environmental Entomology
Zhenwei Zhang, Liming Liu, Huijie Wu, Lifeng Liu, Baoshan Kang, Bin Peng, Qinsheng Gu
Cucumber green mottle mosaic virus (CGMMV) is one of the most devastating viruses infecting members of the family Cucurbitaceae. The assembly initiation site of CGMMV is located in the coding region of the coat protein, which is not only involved in virion assembly but is also a key factor determining the long-distance movement of the virus. To understand the effect of assembly initiation site and the adjacent region on CGMMV infectivity, we created a GTT deletion mutation in the GAGGTTG assembly initiation site of the infectious clone of CGMMV, which we termed V97 (deletion mutation at residue 97 of coat protein), followed by the construction of the V94A and T104A mutants...
December 25, 2017: Viruses
Yanbing Niu, Xiaojing Pang, Yuanyuan Cheng, Defu Wang, Shang Guo, Yong Liu
With continued expansion of Cucurbita pepo L. cultivation, viral diseases affecting the crop have become more serious in recent years, causing enormous losses in yield and quality. A virus sample was obtained from Wenshui in Shanxi province, China. Double-stranded RNA technology and sequence-independent amplification (SIA) were used to identify the virus that induced C. pepo L. mosaic disease. SIA and sequencing results showed the presence of watermelon mosaic virus (WMV) in diseased C. pepo L. leaves. The complete sequence of WMV from the Shanxi isolate (i...
September 2017: Virusdisease
G Angelella, V Nalam, P Nachappa, J White, I Kaplan
Insect endosymbionts (hereafter, symbionts) can modify plant virus epidemiology by changing the physiology or behavior of vectors, but their role in nonpersistent virus pathosystems remains uninvestigated. Unlike propagative and circulative viruses, nonpersistent plant virus transmission occurs via transient contamination of mouthparts, making direct interaction between symbiont and virus unlikely. Nonpersistent virus transmission occurs during exploratory intracellular punctures with styletiform mouthparts when vectors assess potential host-plant quality prior to phloem feeding...
December 30, 2017: Microbial Ecology
Xiaodong Li, Mengnan An, Zihao Xia, Xiaojiao Bai, Yuanhua Wu
Cucumber green mottle mosaic virus (CGMMV) belongs to the Tobamovirus genus and is a major global plant virus on cucurbit plants. It causes severe disease symptoms on infected watermelon plants (Citrullus lanatus), particularly inducing fruit decay. However, little is known about the molecular mechanism of CGMMV-induced watermelon fruit decay. For this study, comparative analysis of transcriptome profiles of CGMMV-inoculated and mock-inoculated watermelon fruits were conducted via RNA-Seq. A total of 1,621 differently expressed genes (DEGs) were identified in CGMMV-inoculated watermelon, among which 1,052 were up-regulated and 569 were down-regulated...
December 1, 2017: Scientific Reports
Yang Sun, Yong Wang, Li Rong Han, Xing Zhang, Jun Tao Feng
In order to develop a novel biofungicide, the antifungal activity and action mode of cuminic acid from the seed of Cuminum cyminum L. against Fusarium oxysporum f. sp. niveum (FON) on watermelon was determined systematically. In this study, the median effective concentration (EC50) value for cuminic acid in inhibiting mycelial growth of FON was 22.53 μg/mL. After treatment with cuminic acid, the mycelial morphology was seriously influenced; cell membrane permeability and glycerol content were increased markedly, but pigment and mycotoxin (mainly fusaric acid) were significantly decreased...
November 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
D Park, H Kim, Y Hahn
Watermelon mosaic virus (WMV) is a member of the genus Potyvirus, which is the largest genus of plant viruses. WMV is a significant pathogen of crop plants, including Cucurbitaceae species. A WMV strain, designated as WMV-Pg, was identified in transcriptome data collected from ginseng (Panax ginseng) root. WMV-Pg showed 84% nucleotide sequence identity and 91% amino acid sequence identity with its closest related virus, WMV-Fr. A phylogenetic analysis of WMV-Pg with other WMVs and soybean mosaic viruses (SMVs) indicated that WMV-Pg is a distinct subtype of the WMV/SMV group of the genus Potyvirus in the family Potyviridae...
2017: Acta Virologica
Muhammad Azher Nawaz, Yanyan Jiao, Chen Chen, Fareeha Shireen, Zuhua Zheng, Muhammad Imtiaz, Zhilong Bie, Yuan Huang
Vanadium (V) is an important heavy metal with ubiquitous presence in the Earth's crust, but limited information is available as to its effect on plants and management strategies. Melatonin is a widely studied biomolecule; it acts as an antioxidant and a signaling molecule that enhances the abiotic stress tolerance of plants. Melatonin improves copper, zinc, and cadmium tolerance in plants. In this study, we investigated the response of watermelon seedlings to V stress and the potential role of melatonin in enhancing V stress tolerance of watermelon seedlings...
November 20, 2017: Journal of Plant Physiology
Mendel Friedman
Cinnamaldehyde is a major constituent of cinnamon essential oils produced by aromatic cinnamon plants. This compound has been reported to exhibit antimicrobial properties in vitro in laboratory media and in animal feeds and human foods contaminated with disease-causing bacteria including Bacillus cereus, Campylobacter jejuni, Clostridium perfringens, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. This integrated review surveys and interprets our current knowledge of the chemistry, analysis, safety, mechanism of action, and antibiotic activities of cinnamaldehyde in food animal (cattle, lambs, calves, pigs, poultry) diets and in widely consumed liquid (apple, carrot, tomato, and watermelon juices, milk) and solid foods...
December 6, 2017: Journal of Agricultural and Food Chemistry
Meng Zhang, Jing Ge, Xiangyang Yu
Fusarium oxysporum: f. sp. niveum (Fusarium oxysporum) causes watermelon wilt, drastically reducing its yield. Thymol (5-methyl-2-(1-methylethyl) phenol, PubChem CID: 6989) has been extensively reported to have antimicrobial activity against pathogenic microorganisms. In this study, the growth of F. oxysporum was significantly inhibited by thymol in vitro. The dry weight of F. oxysporum was decreased significantly. Thymol-induced cell membrane damage was observed at 24 hpi (hours post-incubation). Therefore, the changes in the gene expression level of F...
November 17, 2017: Current Microbiology
Waqar Ahmed Siddiqui, Muhammad Shahzad, Arham Shabbir, Ali Ahmad
Traditionally, Citrullus lanatus is known to have protective properties in kidney diseases and for having the ability to clear urine. Current study aims to validate the traditional uses of C. lanatus by evaluation of anti-urolithiatic and diuretic activities using in vivo and in vitro experiments. Male Wistar rats were used for in vivo anti-urolithiatic and diuretic activities. Supersaturated solution of calcium and oxalate was used for in vitro crystallization study. Hematoxylin & eosin staining was used for histopathological evaluation of kidney...
November 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Zan Li, Jun Jia, Mingwei Wang, Hua Zhang, Hongyuan Yan, Fengxia Qiao
A C8-and-amino-bifunctionalized ordered mesoporous organosilica (C8-AMS) was prepared in a deep eutectic solvent-a green solvent-that was used as the reaction medium for this procedure instead of organic solvents or other catalyst for the first time. This method provided a nontoxic way for C8 grafting and obtaining an ordered mesoporous organosilica material with both hydrophilic and hydrophobic groups. Besides advantages such as its large surface area, regular and uniform pore size, and hydrothermal stability, the obtained bifunctionalized C8-AMS also have two main functional groups in the obtained structure, C8 and amino, which enriched the adsorption mechanism of the material and as a result improved the adsorption selectivity of C8-AMS...
October 31, 2017: Journal of Chromatography. A
Yi Ren, Shaogui Guo, Jie Zhang, Hongju He, Honghe Sun, Shouwei Tian, Guoyi Gong, Haiying Zhang, Amnon Levi, Yaakov Tadmor, Yong Xu
How sugar transporters regulate sugar accumulation in fruits is poorly understood and particularly so for species storing high concentration sucrose. Accumulation of soluble sugars in watermelon (Citrullus lanatus) fruit, a major quality trait, had been selected during domestication. Still, the molecular mechanisms controlling this quantitative trait are unknown. We resequenced 96 recombinant inbred lines, derived from crossing sweet and unsweet accessions, to narrow down the size of a previously described sugar content quantitative trait locus, which contains a putative Tonoplast Sugar Transporter gene (ClTST2)...
November 8, 2017: Plant Physiology
Rohan Mandaliya, Tenzin Choden, Arul Thomas
No abstract text is available yet for this article.
December 2017: Gastroenterology
Sun-Ju Rhee, Taehyung Kwon, Minseok Seo, Yoon Jeong Jang, Tae Yong Sim, Seoae Cho, Sang-Wook Han, Gung Pyo Lee
The whole-genome sequence of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai), a valuable horticultural crop worldwide, was released in 2013. Here, we compared a de novo-based approach (DBA) to a reference-based approach (RBA) using RNA-seq data, to aid in efforts to improve the annotation of the watermelon reference genome and to obtain biological insight into male-sterility in watermelon. We applied these techniques to available data from two watermelon lines: the male-sterile line DAH3615-MS and the male-fertile line DAH3615...
2017: PloS One
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