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https://www.readbyqxmd.com/read/28917590/karyotype-stability-and-unbiased-fractionation-in-the-paleo-allotetraploid-cucurbita-genomes
#1
Honghe Sun, Shan Wu, Guoyu Zhang, Chen Jiao, Shaogui Guo, Yi Ren, Jie Zhang, Haiying Zhang, Guoyi Gong, Zhangcai Jia, Fan Zhang, Jiaxing Tian, William J Lucas, Jeff J Doyle, Haizhen Li, Zhangjun Fei, Yong Xu
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago (Mya), and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C...
September 13, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28893328/plasticity-in-host-utilization-by-two-host-associated-populations-of-aphis-gossypii-glover
#2
A K Barman, K R Gadhave, B Dutta, R Srinivasan
Biological and morphological plasticity in polyphagous insect herbivores allow them to exploit diverse host plant species. Geographical differences in resource availability can lead to preferential host exploitation and result in inconsistent host specialization. Biological and molecular data provide insights into specialization and plasticity of such herbivore populations. In agricultural landscapes, Aphis gossypii encounters several crop and non-crop hosts, which exist in temporal and spatial proximity. We investigated the host-specialization of two A...
September 12, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28878500/genome-wide-identification-of-saur-genes-in-watermelon-citrullus-lanatus
#3
Na Zhang, Xing Huang, Yaning Bao, Bo Wang, Hongxia Zeng, Weishun Cheng, Mi Tang, Yuhua Li, Jian Ren, Yuhong Sun
The early auxin responsive SAUR family is an important gene family in auxin signal transduction. We here present the first report of a genome-wide identification of SAUR genes in watermelon genome. We successfully identified 65 ClaSAURs and provide a genomic framework for future study on these genes. Phylogenetic result revealed a Cucurbitaceae-specific SAUR subfamily and contribute to understanding of the evolutionary pattern of SAUR genes in plants. Quantitative RT-PCR analysis demonstrates the existed expression of 11 randomly selected SAUR genes in watermelon tissues...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28858241/preparation-and-characterization-of-novel-cationic-chitosan-derivatives-bearing-quaternary-ammonium-and-phosphonium-salts-and-assessment-of-their-antifungal-properties
#4
Wenqiang Tan, Qing Li, Fang Dong, Qiuhong Chen, Zhanyong Guo
Chitosan is an abundant and renewable polysaccharide, its derivatives exhibit attractive bioactivities and the wide applications in various biomedical fields. In this paper, two novel cationic chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized via trimethylation, chloride acetylation, and quaternization with tricyclohexylphosphine and triphenylphosphine. The structures and properties of synthesized products in the reactions were characterized by FTIR spectroscopy, ¹H-NMR, (31)P-NMR, elemental and thermogravimetric analysis...
August 31, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28854650/host-mediated-effects-of-semipersistently-transmitted-squash-vein-yellowing-virus-on-sweetpotato-whitefly-hemiptera-aleyrodidae-behavior-and-fitness
#5
Deepak Shrestha, Heather J McAuslane, Scott T Adkins, Hugh A Smith, Nicholas Dufault, James Colee, Susan E Webb
Plant viruses may indirectly affect insect vector behavior and fitness via a shared host plant. Here, we evaluated the host-mediated effects of Squash vein yellowing virus (SqVYV) on the behavior and fitness of its whitefly vector, Bemisia tabaci (Gennadius) Middle East-Asia Minor 1, formerly biotype B. Alighting, settling, and oviposition behavioral assays were conducted on infected and mock-inoculated squash (Cucurbita pepo L.) and watermelon [Citrullus lanatus (Thunb) Matsum and Nakai] plants. Developmental time of immature stages, adult longevity, and fecundity were measured on infected and mock-inoculated squash plants...
August 1, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28843048/stereoselective-determination-of-dufulin-in-watermelon-under-field-conditions-using-chiral-ultra-high-performance-liquid-chromatography-with-high-resolution-mass-spectrometry
#6
Kunming Zheng, Jin Gong, Xiaoqin Li, Guifei Jia, Sizhuo Wu, Haizhen Zhang, Deyu Hu, Kankan Zhang
An effective and sensitive chiral analytical method was established to investigate the stereoselective dissipation of rac-dufulin in watermelon using ultra-high-performance liquid chromatography with a Superchiral S-OD chiral column (4.6 mm × 150 mm i.d., 5 μm) coupled with high-resolution mass spectrometry. To optimize the pretreatment method for detecting rac-dufulin in the three matrices, different extraction solvents, extractant volumes, extraction times, and absorbents were investigated to improve extraction efficiency...
August 26, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28840434/robust-rnai-mediated-resistance-to-infection-of-seven-potyvirids-in-soybean-expressing-an-intron-hairpin-nib-rna
#7
Xiangdong Yang, Lu Niu, Wei Zhang, Hongli He, Jing Yang, Guojie Xing, Dongquan Guo, Qian Du, Xueyan Qian, Yao Yao, Qiyun Li, Yingshan Dong
Viral pathogens, such as soybean mosaic virus (SMV), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. Engineering resistance by RNAi-mediated gene silencing is a powerful strategy for controlling viral diseases. In this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, NIb) gene was isolated from the SMV SC3 strain, driven by the leaf-specific rbcS2 promoter from Phaseolus vulgaris, and introduced into soybean. The transgenic lines had significantly lower average disease indices (ranging from 2...
August 24, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28820117/mucilaginibacter-rubeus-sp-nov-isolated-from-rhizosphere-soil
#8
Jie-Chao Wei, Le-Ni Sun, Zhuo-Xin Yuan, Xue-Ting Hou, En-Dong Yang, Yuan-Yuan Cao
A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated EF23T, was isolated from rhizosphere soil of watermelon. Growth of strain EF23T was observed at 10-37 °C, at pH 5.0-9.0 and in the presence of 0-0.5 % (w/v) NaCl. Strain EF23T contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16:1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH as the major fatty acids. Phosphatidylethanolamine was identified as the major polar lipid...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28811758/pathological-interrelations-of-soil-borne-diseases-in-cucurbits-caused-by-fusarium-species-and-meloidogyne-incognita
#9
Yunhee Seo, Young Ho Kim
Pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by Fusarium isolates (FI) and the root-knot nematode (RKN), Meloidogyne incognita were characterized by the fusarium disease severity index (DI), RKN gall index (GI) and eggmass index (EI) in inoculation tests using FI and RKN. Virulence of FI as determined by DI at 4 weeks after inoculation was mostly in the higher order of Fusarium proliferatum F6, F5 and Fusarium oxysporum f. sp...
August 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28811756/pseudomonas-oleovorans-strain-kbpf-004-culture-supernatants-reduced-seed-transmission-of-cucumber-green-mottle-mosaic-virus-and-pepper-mild-mottle-virus-and-remodeled-aggregation-of-126-kda-and-subcellular-localization-of-movement-protein-of-pepper-mild-mottle
#10
Nam-Gyu Kim, Eun-Young Seo, Sang-Hyuk Han, Jun-Su Gong, Cheol-Nam Park, Ho-Seop Park, Leslie L Domier, John Hammond, Hyoun-Sub Lim
Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudomonas oleovorans strain KBPF-004 (developmental code KNF2016) which showed antiviral activity against mechanical transmission of tobamoviruses...
August 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28810866/the-development-of-an-ivermectin-based-attractive-toxic-sugar-bait-atsb-to-target-anopheles-arabiensis
#11
Frank Chelestino Tenywa, Athumani Kambagha, Adam Saddler, Marta Ferreira Maia
BACKGROUND: An increasing number of countries in sub-Saharan Africa are moving towards malaria-elimination, mostly thanks to successful vector control campaigns. However, elimination has proven challenging, resulting in the persistence of malaria transmission. It is now accepted that in order to eliminate malaria, new complementary vector control approaches must be developed. This study describes the development of a sugar-baited resting place containing a toxic dose of ivermectin for the control of Anopheles arabiensis...
August 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28763978/biochemical-and-histological-contributions-to-textural-changes-in-watermelon-fruit-modulated-by-grafting
#12
Georgios A Soteriou, Anastasios S Siomos, Dimitrios Gerasopoulos, Youssef Rouphael, Soteria Georgiadou, Marios C Kyriacou
Increased watermelon fruit flesh firmness is systematically incurred with grafting on Cucurbita hybrid rootstocks (heterografting). Possible differences in mesocarp cell wall constitution and histology between heterografted, homeografted (self-grafted) and non-grafted watermelon were examined, as well as their contributions to fruit texture. Firmness correlated positively (r=0.78, p<0.001) with cell density (cellsmm(-2)) which was higher in heterografts (5.83) than homeografts (4.64) and non-grafted controls (4...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28756582/rnai-mediated-smv-p3-cistron-silencing-confers-significantly-enhanced-resistance-to-multiple-potyvirus-strains-and-isolates-in-transgenic-soybean
#13
Xiangdong Yang, Lu Niu, Wei Zhang, Jing Yang, Guojie Xing, Hongli He, Dongquan Guo, Qian Du, Xueyan Qian, Yao Yao, Qiyun Li, Yingshan Dong
Robust RNAi-mediated resistance to multiple Potyvirus strains and isolates, but not to Secovirus BPMV, was conferred by expressing a short SMV P3 hairpin in soybean plants. Engineering resistance to multiple Potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. In this study, RNAi-mediated silencing of the soybean mosaic virus (SMV) P3 cistron, which is reported to participate in virus movements and pathogenesis and to be the putative determinant of SMV virulence, was used to induce resistance to multiple Potyvirus strains and isolates in soybean...
July 29, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28753059/growth-of-salmonella-spp-and-escherichia-coli-o157-h7-on-fresh-cut-fruits-stored-at-different-temperatures
#14
Ke Feng, Wenzhong Hu, Aili Jiang, Gaowa Saren, Yongping Xu, Yaru Ji, Wenjun Shao
The objective of this work was to determine the growth potential of Salmonella spp. and Escherichia coli O157:H7 on fresh-cut honeydew melon, cantaloupe, watermelon, pitaya, mango, papaya, and pineapple stored at 5°C, 13°C, and 25°C. The results showed that both pathogens were able to grow on fresh-cut fruits except fresh-cut pineapple at 13°C and 25°C. Salmonella spp. grew more rapidly on fresh-cut honeydew melon, cantaloupe, watermelon, and mango than did E. coli O157:H7 at 13°C. The growth of both species was inhibited on fresh-cut pineapple, with that of Salmonella spp...
July 28, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28744150/radiofrequency-ablation-using-barrx-%C3%A2-for-the-endoscopic-treatment-of-gastric-antral-vascular-ectasia-a-series-of-three-cases-and-a-review-of-the-literature-on-treatment-options
#15
Anish Patel, Sunil Patel, Prasanna C Wickremesinghe, Deepak Vadada
Gastric antral vascular ectasia (GAVE), also known as "watermelon stomach", is an uncommon condition, which can cause gastrointestinal bleeding due to rupture of blood vessels that line the stomach. The pathogenesis of GAVE remains unclear; however it is thought that hemodynamic changes, mechanical stress, and autoimmune factors all have a part to play. A range of conditions are also commonly associated with the syndrome, such as portal hypertensive gastropathy, liver cirrhosis, and autoimmune disorders. Less commonly, chronic renal failure, cardiac diseases, and bone marrow transplantation have coexisted with GAVE...
2017: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/28741223/citrulline-metabolism-in-plants
#16
REVIEW
Vijay Joshi, Alisdair R Fernie
Citrulline was chemically isolated more than 100 years ago and is ubiquitous in animals, plants, bacteria, and fungi. Most of the research on plant citrulline metabolism and transport has been carried out in Arabidopsis thaliana and the Cucurbitaceae family, particularly in watermelon which accumulates this non-proteinogenic amino acid to very high levels. Industrially, citrulline is produced via specially optimized microbial strains; however, the amounts present in watermelon render it an economically viable source providing that other high-value compounds can be co-extracted...
July 25, 2017: Amino Acids
https://www.readbyqxmd.com/read/28704497/genetic-mapping-of-the-lobed-leaf-1-clll1-gene-to-a-127-6-kb-region-in-watermelon-citrullus-lanatus-l
#17
Chunhua Wei, Xiner Chen, Zhongyuan Wang, Qiyan Liu, Hao Li, Yong Zhang, Jianxiang Ma, Jianqiang Yang, Xian Zhang
The lobed leaf character is a unique morphologic trait in crops, featuring many potential advantages for agricultural productivity. Although the majority of watermelon varieties feature lobed leaves, the genetic factors responsible for lobed leaf formation remain elusive. The F2:3 leaf shape segregating population offers the opportunity to study the underlying mechanism of lobed leaf formation in watermelon. Genetic analysis revealed that a single dominant allele (designated ClLL1) controlled the lobed leaf trait...
2017: PloS One
https://www.readbyqxmd.com/read/28690629/genome-wide-analysis-of-simple-sequence-repeats-in-bitter-gourd-momordica-charantia
#18
Junjie Cui, Jiaowen Cheng, Dingguo Nong, Jiazhu Peng, Yafei Hu, Weiming He, Qianjun Zhou, Narinder P S Dhillon, Kailin Hu
Bitter gourd (Momordica charantia) is widely cultivated as a vegetable and medicinal herb in many Asian and African countries. After the sequencing of the cucumber (Cucumis sativus), watermelon (Citrullus lanatus), and melon (Cucumis melo) genomes, bitter gourd became the fourth cucurbit species whose whole genome was sequenced. However, a comprehensive analysis of simple sequence repeats (SSRs) in bitter gourd, including a comparison with the three aforementioned cucurbit species has not yet been published...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28681144/the-complete-nucleotide-sequence-and-genome-organization-of-a-novel-betaflexivirus-infecting-citrullus-lanatus
#19
Min Xin, Peipei Zhang, Wenwen Liu, Yingdang Ren, Mengji Cao, Xifeng Wang
The complete nucleotide sequence of a novel positive single-stranded (+ss) RNA virus, tentatively named watermelon virus A (WVA), was determined using a combination of three methods: RNA sequencing, small RNA sequencing, and Sanger sequencing. The full genome of WVA is comprised of 8,372 nucleotides (nt), excluding the poly (A) tail, and contains four open reading frames (ORFs). The largest ORF, ORF1 encodes a putative replication-associated polyprotein (RP) with three conserved domains. ORF2 and ORF4 encode a movement protein (MP) and coat protein (CP), respectively...
July 5, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28672690/valorization-of-starchy-cellulosic-and-sugary-food-waste-into-hydroxymethylfurfural-by-one-pot-catalysis
#20
Iris K M Yu, Daniel C W Tsang, Alex C K Yip, Season S Chen, Yong Sik Ok, Chi Sun Poon
This study aimed to produce a high-value platform chemical, hydroxymethylfurfural (HMF), from food waste and evaluate the catalytic performance of trivalent and tetravalent metals such as AlCl3, CrCl3, FeCl3, Zr(O)Cl2, and SnCl4 for one-pot conversion. Starchy food waste, e.g., cooked rice and penne produced 4.0-8.1 wt% HMF and 46.0-64.8 wt% glucose over SnCl4 after microwave heating at 140 °C for 20 min. This indicated that starch hydrolysis was effectively catalyzed but subsequent glucose isomerization was rate-limited during food waste valorization, which could be enhanced by 40-min reaction to achieve 22...
October 2017: Chemosphere
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