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https://www.readbyqxmd.com/read/28386471/characterization-of-virulence-potential-of-pseudomonas-aeruginosa-isolated-from-bovine-meat-fresh-fish-and-smoked-fish
#1
Comoé Koffi Donatien Benie, Adjéhi Dadié, Nathalie Guessennd, Nadège Ahou N'gbesso-Kouadio, N'zebo Désiré Kouame, David Coulibaly N'golo, Solange Aka, Etienne Dako, Koffi Marcellin Dje, Mireille Dosso
Pseudomonas aeruginosa owns a variability of virulence factors. These factors can increase bacterial pathogenicity and infection severity. Despite the importance of knowledge about them, these factors are not more characterized at level of strains derived from local food products. This study aimed to characterize the virulence potential of P. aeruginosa isolated from various animal products. Several structural and virulence genes of P. aeruginosa including lasB, exoS, algD, plcH, pilB, exoU, and nan1 were detected by polymerase chain reaction (PCR) on 204 strains of P...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28315724/molecular-detection-of-six-virulence-genes-in-pseudomonas-aeruginosa-isolates-detected-in-children-with-urinary-tract-infection
#2
Ali Badamchi, Hossein Masoumi, Shima Javadinia, Ramin Asgarian, Azardokht Tabatabaee
Although a vast majority of Urinary tract infections (UTIs) are caused by E. coli, epidemiological reports have indicated an increasing rate of such infections caused by some other opportunistic organisms including Pseudomonas aeruginosa. Antimicrobial susceptibility and pathogenesis mechanisms of P. aeruginosa are poorly understood. The aim of this study was to detect some virulence factor genes and antimicrobial susceptibility patterns of P. aeruginosa isolates detected in patients with UTI, in children hospital of Tehran, Tehran, Iran...
March 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28298909/fingerprint-analysis-and-identification-of-strains-st309-as-a-potential-high-risk-clone-in-a-pseudomonas-aeruginosa-population-isolated-from-children-with-bacteremia-in-mexico-city
#3
Rosario Morales-Espinosa, Gabriela Delgado, Luis F Espinosa, Dassaev Isselo, José L Méndez, Cristina Rodriguez, Guadalupe Miranda, Alejandro Cravioto
Pseudomonas aeruginosa is an opportunistic pathogen and is associated with nosocomial infections. Its ability to thrive in a broad range of environments is due to a large and diverse genome of which its accessory genome is part. The objective of this study was to characterize P. aeruginosa strains isolated from children who developed bacteremia, using pulse-field gel electrophoresis, and in terms of its genomic islands, virulence genes, multilocus sequence type, and antimicrobial susceptibility. Our results showed that P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28225287/-predatory-journals-how-their-publishers-operate-and-how-to-avoid-them
#4
Jiří Kratochvíl, Lukáš Plch
Authors who publish in scientific or scholarly journals today face the risk of publishing in so-called predatory journals. These journals exploit the noble idea of the Open Access movement, whose goal is to make the latest scientific findings available for free. Predatory journals, unlike the reputable ones working on an Open Access basis, neglect the review process and publish low-quality submissions. The basic attributes of predatory journals are a very quick review process or even none at all, failure to be transparent about author fees for publishing an article, misleading potential authors by imitating the names of well-established journals, and false information on indexing in renowned databases or assigned impact factor...
2017: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28220299/molecular-analysis-of-braf-v600e-mutation-in-multiple-nodules-of-pulmonary-langerhans-cell-histiocytosis
#5
Arno Dimmler, Helene Geddert, Martin Werner, Gerhard Faller
Pulmonary Langerhans cell histiocytosis (PLCH) is a rare, smoking-related histiocytic disorder with variable clinical symptoms. Like in other non-pulmonary Langerhans cell proliferations, PLCH has recently been shown to harbour BRAF V600E mutations in a significant subset of cases, thus challenging the concept of PLCH being a reactive disorder. Here, we analysed 38 formalin-fixed and paraffin-embedded PLCH nodules of nine patients for BRAF mutation using two different molecular methods. Using pyrosequencing and allele-specific quantitative PCR (AS-PCR), BRAF V600E mutations were found in 16/38 (42%) and 31/37 (84%) nodules, respectively...
April 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28038909/beta-lactamase-producing-pseudomonas-aeruginosa-phenotypic-characteristics-and-molecular-identification-of-virulence-genes
#6
Waheed Ullah, Muhammad Qasim, Hazir Rahman, Yan Jie, Noor Muhammad
BACKGROUND: Pseudomonas aeruginosa causes common infections in immunocompromised and cystic fibrosis patients. However, drug resistance capability and release of virulence factors play a key role in bacterial pathogenicity. METHODS: Beta-lactamase-producing clinical isolates of P. aeruginosa were screened for biofilm formation and pigment production. Subsequently, all the isolates were subjected to the detection of six virulence genes (OprI, OprL, LasB, PlcH, ExoS, and ToxA)...
March 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/27737563/chest-computed-tomographic-image-screening-for-cystic-lung-diseases-in-patients-with-spontaneous-pneumothorax-is-cost-effective
#7
Nishant Gupta, Dale Langenderfer, Francis X McCormack, Daniel P Schauer, Mark H Eckman
RATIONALE: Patients without a known history of lung disease presenting with a spontaneous pneumothorax are generally diagnosed as having primary spontaneous pneumothorax. However, occult diffuse cystic lung diseases such as Birt-Hogg-Dubé syndrome (BHD), lymphangioleiomyomatosis (LAM), and pulmonary Langerhans cell histiocytosis (PLCH) can also first present with a spontaneous pneumothorax, and their early identification by high-resolution computed tomographic (HRCT) chest imaging has implications for subsequent management...
January 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/27692140/bronchoalveolar-lavage-for-the-diagnosis-of-pulmonary-langerhans-cell-histiocytosis
#8
Marek Lommatzsch, Kai Bratke, Paul Stoll, Norbert Mülleneisen, Friedrich Prall, Andrea Bier, J Christian Virchow
BACKGROUND: The histologic diagnosis of Pulmonary Langerhans cell histiocytosis (PLCH) is invasive and can cause complications. To confirm the diagnosis of PLCH, guidelines therefore recommend measuring CD1a-positive bronchoalveolar lavage fluid (BALF) cells despite its poor sensitivity and specificity. Thus, an improved diagnostic accuracy of BALF cell analysis would be desirable. METHODS: Using four-colour flow cytometry, plasmacytoid and myeloid dendritic cells (DCs) were analysed in BALF of 10 newly diagnosed, untreated, smoking patients with PLCH, and compared with BALF DCs from 40 asymptomatic smokers and 21 never-smokers...
October 2016: Respiratory Medicine
https://www.readbyqxmd.com/read/27457974/molecular-detection-of-virulence-genes-in-pseudomonas-aeruginosa-isolated-from-children-with-cystic-fibrosis-and-burn-wounds-in-iran
#9
COMPARATIVE STUDY
Fatemeh Faraji, Mohammadreza Mahzounieh, Azizollah Ebrahimi, Fatemeh Fallah, Omid Teymournejad, Behnaz Lajevardi
Pseudomonas aeruginosa possesses various virulence factors which contribute to the bacterial invasion and toxicity. Moreover, children suffered from Cystic Fibrosis (CF) and burn wounds are at a high risk of various bacterial infections. The aim of this study was to determine the prevalence of virulent genes in P. aeruginosa isolated from children with CF and burn wounds and comparing their virulence genes to figure out the role of every virulent factor in the infections. P. aeruginosa were isolated from sputum, oropharyngeal swabs, and broncho-alveolar lavage (BAL) specimens from CF and burn wounds between June 2013 and June 2014 in Tehran's hospitals...
October 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27330952/long-term-improvement-during-tadalafil-therapy-in-a-patient-with-pulmonary-hypertension-secondary-to-pulmonary-langerhans-cell-histiocytosis
#10
Kenji Nemoto, Shuji Oh-Ishi, Toshihide Inui, Mariko Nakazawa, Kentaro Hyodo, Masayuki Nakajima, Jun Kanazawa, Yukiko Miura, Takio Takaku, Yuko Minami, Kenji Hayashihara, Takefumi Saito, Yoshinori Kawabata
Pulmonary arterial hypertension (PAH) secondary to pulmonary Langerhans cell histiocytosis (PLCH) is known to be a relatively common complication and is associated with a poor prognosis. However, the optimal therapeutic approach for these cases remains to be established. A 57-year-old man visited our hospital because of a progressive dry cough. A thoracic computed tomography examination showed a combination of diffuse thick-walled cysts and reticulonodular shadows that were predominant in bilateral upper lobes of the lungs...
2016: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/27293848/pulmonary-langerhans-cell-histiocytosis-analysis-of-14-patients-and-literature-review
#11
Cheng-Wei Li, Man-Hui Li, Jiang-Xiong Li, Ru-Jia Tao, Jin-Fu Xu, Wei-Jun Cao
BACKGROUND: Pulmonary Langerhans cell histiocytosis (PLCH) is an orphan disease in respiratory medicine, which most affects adult smokers. The purpose of this article was to discuss the clinical features, especially the radiologic features of PLCH patients during their hospitalization through a retrospective analysis on clinical data. Furthermore, the current literature was also reviewed. METHODS: Between December 2008 and June 2012, 14 patients with PLCH were assessed at Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China...
June 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27222791/diffuse-cystic-lung-disease-due-to-pulmonary-metastasis-of-colorectal-carcinoma
#12
Mariano Fielli, Fabio Avila, Agustina Saino, Deborah Seimah, Marcelo Fernández Casares
The diffuse cystic lung diseases (DCLDs) are a pathophysiologically heterogeneous processes characterized by the presence of multiple thin-walled, air-filled spaces within the pulmonary parenchyma. The most common causes of DCLD are lymphangioleiomyomatosis (LAM) and pulmonary Langerhans cell histiocytosis (PLCH). DCLD develops rarely as a result of malignancy, typically secondary to metastases from peripheral sarcomas and mesenchymal tumors. DCLD have also been reported in a variety of other metastatic disease such as adenocarcinoma...
2016: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/27169367/virulence-and-resistance-features-of-pseudomonas-aeruginosa-strains-isolated-from-chronic-leg-ulcers
#13
Mihaela Georgescu, Irina Gheorghe, Carmen Curutiu, Veronica Lazar, Coralia Bleotu, Mariana-Carmen Chifiriuc
BACKGROUND: The purpose of this study was to evaluate the virulence profiles of Pseudomonas aeruginosa clinical strains recently isolated from patients hospitalized for chronic leg ulcers in the Dermatology Department of Central Military Emergency University Hospital "Carol Davila", Bucharest, Romania. METHODS: The phenotypic screening evaluated eight soluble virulence factors (haemolysins, lecithinase, lipase, caseinase, gelatinase, amylase, DNase, aesculin hydrolysis), as well as adherence ability (Cravioto adapted method) and invasion capacity on HeLa cells (gentamicin protection assay)...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27107526/virulence-genes-profile-of-multidrug-resistant-pseudomonas-aeruginosa-isolated-from-iranian-children-with-utis
#14
Zohreh Heidary, Eshagh Bandani, Mohamad Eftekhary, Ali Akbar Jafari
Virulent and resistant strains Pseudomonas aeruginosa (P. aeruginosa) is one of the most important cause of UTIs in pediatrics. The present study was carried to investigate the frequency of virulence factors in the multi-drug resistant strains of P. aeruginosa isolated from pediatrics hospitalized due to the UTIs. One-hundred and forty three urine samples were collected from pediatric patients suffered from UTIs. Samples were cultured and those that were P. aeruginosa positive were analyzed for the presence of putative virulence genes...
March 2016: Acta Medica Iranica
https://www.readbyqxmd.com/read/26935369/pulmonary-langerhans-cell-histiocytosis-associated-pulmonary-hypertension-showing-a-drastic-improvement-following-smoking-cessation
#15
Yoshiaki Kinoshita, Kentaro Watanabe, Atsuhiko Sakamoto, Kouko Hidaka
Pulmonary Langerhans cell histiocytosis (PLCH) is a rare, smoking-related, interstitial lung disease, and pulmonary hypertension (PH) is associated with mortality. We herein report a case of PLCH complicated by severe PH and respiratory impairment. After developing PH, the patient displayed a cystic pattern on chest high-resolution computed tomography (HRCT). This, in turn, corresponded with the scarring stage of PLCH. However, the patient's PH and respiratory impairment improve dramatically following smoking cessation...
2016: Internal Medicine
https://www.readbyqxmd.com/read/26927717/pulmonary-langerhans-cell-histiocytosis-an-update-from-the-pathologists-perspective
#16
REVIEW
Anja C Roden, Eunhee S Yi
CONTEXT: Pulmonary Langerhans cell histiocytosis (PLCH) is a rare histiocytic disorder that almost exclusively affects the lungs of smokers. PLCH is characterized by bronchiolocentric nodules and/or cysts in an upper and mid lung distribution with sparing of the costophrenic angles. The diagnosis can be challenging and often requires transbronchial biopsy or surgical lung biopsy. Pulmonary hypertension is a relatively common and sometimes severe complication of PLCH. The pathogenesis of PLCH is still debated...
March 2016: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/26915300/mutually-exclusive-extracellular-signal-regulated-kinase-pathway-mutations-are-present-in-different-stages-of-multi-focal-pulmonary-langerhans-cell-histiocytosis-supporting-clonal-nature-of-the-disease
#17
Michal Kamionek, Parnian Ahmadi Moghaddam, Ali Sakhdari, Alexandra E Kovach, Matthew Welch, Xiuling Meng, Karen Dresser, Keith Tomaszewicz, Ediz F Cosar, Eugene J Mark, Armando E Fraire, Lloyd Hutchinson
AIMS: Pulmonary Langerhans cell histiocytosis (PLCH) is an idiopathic cigarette smoking-related disorder of the lung. Molecular changes in cellular or fibrotic stages of PLCH have not been investigated. We studied the prevalence of extracellular signal-regulated kinase (ERK) pathway mutations in different PLCH stages and other non-PLCH smoking-related lung diseases. METHODS AND RESULTS: The cohort included 28 PLCH with cellular (n = 10), mixed cellular/fibrotic (n = 4) and fibrotic histology (n = 14)...
September 2016: Histopathology
https://www.readbyqxmd.com/read/26833097/extrathoracic-investigation-in-adult-patients-with-isolated-pulmonary-langerhans-cell-histiocytosis
#18
MULTICENTER STUDY
Abdellatif Tazi, Constance de Margerie-Mellon, Laetitia Vercellino, Jean Marc Naccache, Stéphanie Fry, Stéphane Dominique, Stéphane Jouneau, Gwenaël Lorillon, Emmanuelle Bugnet, Raphael Chiron, Benoit Wallaert, Dominique Valeyre, Sylvie Chevret
BACKGROUND: An important objective on diagnosis of patients with Langerhans cell histiocytosis (LCH) is to determine the extent of disease. However, whether systematic extrathoracic investigation is needed in adult patients with clinically isolated pulmonary LCH (PLCH) has not been evaluated. METHODS: In this prospective, multicentre study, 54 consecutive patients with newly diagnosed clinically isolated PLCH were systematically evaluated at inclusion by bone imaging and blood laboratory testing to search for subclinical extrapulmonary LCH involvement...
February 2, 2016: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/26424789/pulmonary-langerhans-cell-histiocytosis-in-pre-existing-chronic-myelomonocytic-leukaemia-a-rare-association
#19
J Temporal, L Armstrong, N Bhatt, A R L Medford
No abstract text is available yet for this article.
March 2016: QJM: Monthly Journal of the Association of Physicians
https://www.readbyqxmd.com/read/26281322/correlation-between-antibiotic-resistance-and-virulence-of-pseudomonas-aeruginosa-clinical-isolates
#20
Fatma Ibrahim Sonbol, Maha Abd El Fattah Khalil, Abdelfattah Badr Mohamed, Sameh Samir Ali
BACKGROUND/AIM: Virulent Pseudomonas aeruginosa. is frequently life-threatening and often challenging to treat, and the emergence of multidrug-resistant isolates presents a critical problem for patients. The aim of the study was concerned with molecular analysis of the virulence factors and antimicrobial resistance profile of multidrug-resistant P. aeruginosa (MDRPA). MATERIALS AND METHODS: Out of 44 MDRPA isolates, 12 isolates representing different resistance profiles and sources of samples were selected for further molecular studies...
2015: Turkish Journal of Medical Sciences
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