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https://www.readbyqxmd.com/read/28257520/lactoferrin-binding-protein-b-a-bi-functional-bacterial-receptor-protein
#1
Nicholas K H Ostan, Rong-Hua Yu, Dixon Ng, Christine Chieh-Lin Lai, Anastassia K Pogoutse, Vladimir Sarpe, Morgan Hepburn, Joey Sheff, Shaunak Raval, David C Schriemer, Trevor F Moraes, Anthony B Schryvers
Lactoferrin binding protein B (LbpB) is a bi-lobed outer membrane-bound lipoprotein that comprises part of the lactoferrin (Lf) receptor complex in Neisseria meningitidis and other Gram-negative pathogens. Recent studies have demonstrated that LbpB plays a role in protecting the bacteria from cationic antimicrobial peptides due to large regions rich in anionic residues in the C-terminal lobe. Relative to its homolog, transferrin-binding protein B (TbpB), there currently is little evidence for its role in iron acquisition and relatively little structural and biophysical information on its interaction with Lf...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28208261/construction-of-aiegens-based-bioprobe-with-two-fluorescent-signals-for-enhanced-monitor-of-extracellular-and-intracellular-telomerase-activity
#2
Yuan Zhuang, Chunli Shang, Xiaoding Lou, Fan Xia
Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of telomerase, two positively charged AIEgens (Silole-R and TPE-H) bind to quencher group labeled primer (QP) and the extension repeated units, leading enhancement of two telomerase-triggered fluorescent signals. Furthermore, by combination the wider linear range in vitro and lower background in living cells imaging, the bioprobe is used to detect telomerase extracted from various cell lines (MCF-7, HeLa, E-J, and HLF), 50 bladder cancer patients' urine samples, 10 normal people's urine samples, and also applied in mapping telomerase activity inside living cells (MCF-7, HeLa, MDA-MB-231, and HT1080)...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28184917/-corrigendum-loss-of-runt%C3%A2-related-transcription-factor%C3%A2-3-induces-resistance-to-5%C3%A2-fluorouracil-and-cisplatin-in-hepatocellular-carcinoma
#3
Junro Kataoka, Hidenori Shiraha, Shigeru Horiguchi, Hiroaki Sawahara, Daisuke Uchida, Teruya Nagahara, Masaya Iwamuro, Hiroki Morimoto, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Shinichiro Nakamura, Akinobu Takaki, Kazuhiro Nouso, Takahito Yagi, Kazuhide Yamamoto, Hiroyuki Okada
Following the publication of this article, we realize that there were some errors in the manuscript. Details of the experiments describing the gene silencing of RUNX3 with small interfering RNA (siRNA) were erroneously included in this paper, and all references to siRNA should have been deleted from the manuscript prior to publication. In the subsection entitled 'Cell lines and cell culture' on page 2577, the left‑hand column, the text should have indicated that the human HCC cell lines Hep3B and Huh7 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), whereas HLF cells were obtained from the Japanese Cancer Resources Bank (Tokyo, Japan)...
March 2017: Oncology Reports
https://www.readbyqxmd.com/read/28178143/healthy-lifestyle-and-normal-waist-circumference-are-associated-with-a-lower-5-year-risk-of-type-2-diabetes-in-middle-aged-and-elderly-individuals-results-from-the-healthy-aging-longitudinal-study-in-taiwan-halst
#4
MULTICENTER STUDY
Chu-Chih Chen, Kiang Liu, Chih-Chen Hsu, Hsing-Yi Chang, Hsiao-Chun Chung, Jih-Shin Liu, Yo-Hann Liu, Tsung-Lung Tsai, Wen-Jin Liaw, I-Ching Lin, Hsi-Wen Wu, Chung-Chou Juan, Hou-Chang Chiu, Marion M Lee, Chao A Hsiung
Type 2 diabetes mellitus (DM) is known to be closely associated with lifestyle and obesity and has a prevalence that increases with age. This study aimed to assess the short-term composite effect of diet, physical activity, psychosocial health, and waist circumference (WC) on the incidence of DM in the elderly and to provide a lifestyle-based predictive index.We used baseline measurements (2009-2013) of 5349 community-dwelling participants (aged 55 years and older, 52% female) of the Healthy Aging Longitudinal Study in Taiwan (HALST) for fasting plasma glucose, HbA1C, serum cholesterol, triglycerides, blood pressures, WC, and outcomes of home-visit questionnaire...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28170415/characterization-of-recombinant-human-lactoferrin-n-glycans-expressed-in-the-milk-of-transgenic-cows
#5
Annabelle Le Parc, Sercan Karav, Camille Rouquié, Elizabeth A Maga, Apichaya Bunyatratchata, Daniela Barile
Lactoferrin (LF) is one of the most abundant bioactive glycoproteins in human milk. Glycans attached through N-glycosidic bonds may contribute to Lactoferrin functional activities. In contrast, LF is present in trace amounts in bovine milk. Efforts to increase LF concentration in bovine milk led to alternative approaches using transgenic cows to express human lactoferrin (hLF). This study investigated and compared N-glycans in recombinant human lactoferrin (rhLF), bovine lactoferrin (bLF) and human lactoferrin by Nano-LC-Chip-Q-TOF Mass Spectrometry...
2017: PloS One
https://www.readbyqxmd.com/read/28169552/lactoferrin-and-the-lactoferrin-sophorolipids-assembly-can-be-internalized-by-dermal-fibroblasts-and-regulate-gene-expression
#6
Rulan Jiang, Yasushi A Suzuki, Xiaogu Du, Bo Lönnerdal
Lactoferrin (Lf) is an iron-binding multifunctional protein, mainly present in external secretions. Lf is known to penetrate skin and may thus exert its multiple functions in skin. Sophorolipids (SLs) are glycolipid biosurfactants, which have been shown to enhance absorption of commercial bovine Lf (CbLf) in model skin via forming an assembly with CbLf. In this study, uptake and post-internalization localization of bovine Lf (bLf), CbLf, and human Lf (hLf) with or without forming assemblies with SLs in human dermal fibroblasts (HDFn) were determined using (125)I-labeled Lfs and confocal microscopy, respectively...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28168749/promising-therapeutic-efficacy-of-a-novel-reic-dkk-3-expressing-adenoviral-vector-for-hepatocellular-carcinoma
#7
Hiroaki Sawahara, Hidenori Shiraha, Daisuke Uchida, Hironari Kato, Ryo Kato, Atsushi Oyama, Teruya Nagahara, Masaya Iwamuro, Shigeru Horiguchi, Koichiro Tsutsumi, Mari Mandai, Tetsushige Mimura, Nozomu Wada, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Shinichiro Nakamura, Masami Watanabe, Masakiyo Sakaguchi, Akinobu Takaki, Kazuhiro Nouso, Takahito Yagi, Yasutomo Nasu, Hiromi Kumon, Hiroyuki Okada
BACKGROUND AND AIM: Reduced expression in immortalized cells (REIC)/dickkopf 3 (Dkk-3) is a tumor suppressor gene that is downregulated in various cancers. In our previous study of prostate cancer, the REIC/Dkk-3-expressing adenoviral vector (Ad-REIC) was found to induce cancer-selective apoptosis. We recently developed a novel super gene expression (SGE) system and used this system to re-construct an Ad-REIC vector, termed the Ad-SGE-REIC, to achieve more effective therapeutic outcomes...
February 6, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28165293/anticancer-activities-of-bovine-and-human-lactoferricin-derived-peptides
#8
Mauricio Arias, Ashley L Hilchie, Evan F Haney, Jan G M Bolscher, M Eric Hyndman, Robert E W Hancock, Hans J Vogel
Lactoferrin (LF) is a mammalian host defense glycoprotein with diverse biological activities. Peptides derived from the cationic region of LF possess cytotoxic activity against cancer cells in vitro and in vivo. Bovine lactoferricin (LFcinB), a peptide derived from bovine LF (bLF), exhibits broad-spectrum anticancer activity, while a similar peptide derived from human LF (hLF) is not as active. In this work, several peptides derived from the N-terminal regions of bLF and hLF were studied for their anticancer activities against leukemia and breast-cancer cells, as well as normal peripheral blood mononuclear cells...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28123551/cell-death-in-a-co-culture-of-hepatocellular-carcinoma-cells-and-human-umbilical-vascular-endothelial-cells-in-a-medium-lacking-glucose-and-arginine
#9
Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige
Human primary hepatocytes are able to survive in a medium without glucose and arginine that is instead supplemented with galactose and ornithine (hepatocyte selection medium; HSM). This is because the cells produce glucose and arginine by the action of galactokinase (GALK) and ornithine carbamoyltransferase (OTC), respectively. It was expected that hepatocellular carcinoma (HCC) cells do not survive in HSM. In the current study, HCC cell lines (namely HLE, HLF, PLC/PRL/5, Hep3B and HepG2) and human umbilical vascular endothelial cells (HUVECs) were cultured in HSM, and the expression levels of GALK1, GALK2 and OTC were analyzed by reverse transcription-quantitative polymerase chain reaction...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/28122742/ex-vivo-drug-response-profiling-detects-recurrent-sensitivity-patterns-in-drug-resistant-all
#10
Viktoras Frismantas, Maria Pamela Dobay, Anna Rinaldi, Joelle Tchinda, Samuel H Dunn, Joachim Kunz, Paulina Richter-Pechanska, Blerim Marovca, Orrin Pail, Silvia Jenni, Ernesto Diaz-Flores, Bill H Chang, Timothy J Brown, Robert H Collins, Sebastian Uhrig, Gnana P Balasubramanian, Obul R Bandapalli, Salome Higi, Sabrina Eugster, Pamela Voegeli, Mauro Delorenzi, Gunnar Cario, Mignon L Loh, Martin Schrappe, Martin Stanulla, Andreas E Kulozik, Martina U Muckenthaler, Vaskar Saha, Julie A Irving, Roland Meisel, Thomas Radimerski, Arend Von Stackelberg, Cornelia Eckert, Jeffrey W Tyner, Peter Horvath, Beat C Bornhauser, Jean-Pierre Bourquin
Drug sensitivity and resistance testing on diagnostic leukemia samples should provide important functional information to guide actionable target and biomarker discovery. We provide proof of concept data by profiling 60 drugs on 68 acute lymphoblastic leukemia (ALL) samples mostly from resistant disease in co-cultures on bone marrow stromal cells. Patient-derived xenografts retained the original pattern of mutations found in the matched patient material. Stromal co-culture did not prevent leukemia cell cycle activity, while a specific sensitivity profile to cell cycle related drugs identified samples with higher cell proliferation both in vitro and in vivo as leukemia xenografts...
January 25, 2017: Blood
https://www.readbyqxmd.com/read/28119096/hawthorn-crataegus-pinnatifida-bunge-leave-flavonoids-attenuate-atherosclerosis-development-in-apoe-knock-out-mice
#11
Pengzhi Dong, Lanlan Pan, Xiting Zhang, Wenwen Zhang, Xue Wang, Meixiu Jiang, Yuanli Chen, Yajun Duan, Honghua Wu, Yantong Xu, Peng Zhang, Yan Zhu
ETHNOPHARMACOLOGICAL RELEVANCE: Hawthorn (Crataegus pinnatifida Bunge) leave have been used to treat cardiovascular diseases in China and Europe. Hawthorn leave flavonoids (HLF) are the main part of extraction. Whether hawthorn leave flavonoids could attenuate the development of atherosclerosis and the possible mechanism remain unknown. MATERIALS AND METHODS: High-fat diet (HFD) mixed with HLF at concentrations of 5mg/kg and 20mg/kg were administered to apolipoprotein E (apoE) knock out mice...
January 21, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28095470/endogenous-semaphorin-7a-impedes-human-lung-fibroblast-differentiation
#12
Stephane Esnault, Elizabeth E Torr, Ksenija Bernau, Mats W Johansson, Elizabeth A Kelly, Nathan Sandbo, Nizar N Jarjour
Semaphorin-7A is a glycosylphosphatidylinositol-anchored protein, initially characterized as an axon guidance protein. Semaphorin-7A also contributes to immune cell regulation and may be an essential pro-fibrotic factor when expressed by non-fibroblast cell types (exogenous). In mouse models, semaphorin-7A was shown to be important for TGF-ß1-induced pulmonary fibrosis characterized by myofibroblast accumulation and extracellular matrix deposition, but the cell-specific role of semaphorin-7A was not examined in fibroblasts...
2017: PloS One
https://www.readbyqxmd.com/read/28079060/intranasal-lactoferrin-enhances-%C3%AE-secretase-dependent-amyloid-precursor-protein-processing-via-the-erk1-2-creb-and-hif-1%C3%AE-pathways-in-an-alzheimer-s-disease-mouse-model
#13
Chuang Guo, Zhao-Hui Yang, Shuai Zhang, Rui Chai, Han Xue, Yan-Hui Zhang, Jia-Yi Li, Zhan-You Wang
Growing evidence suggests that lactoferrin (Lf), an iron-binding glycoprotein, is a pleiotropic functional nutrient. Additionally, Lf was recently implicated as a neuroprotective agent. These properties make Lf a valuable therapeutic candidate for the treatment of Alzheimer's disease (AD). However, the mechanisms regulating the physiological roles of Lf in the pathologic condition of AD remain unknown. In the present study, an APPswe/PS1DE9 transgenic mouse model of AD was used. We explored whether intranasal human Lf (hLf) administration could reduce β-amyloid (Aβ) deposition and ameliorate cognitive decline in this AD model...
January 12, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28052017/rhcg-and-tcaf1-promoter-hypermethylation-predicts-biochemical-recurrence-in-prostate-cancer-patients-treated-by-radical-prostatectomy
#14
Siri H Strand, Michal Switnicki, Mia Moller, Christa Haldrup, Tine M Storebjerg, Jakob Hedegaard, Iver Nordentoft, Soren Hoyer, Michael Borre, Jakob S Pedersen, Peter J Wild, Jong Y Park, Torben F Orntoft, Karina D Sorensen
PURPOSE: The lack of biomarkers that can distinguish aggressive from indolent prostate cancer has caused substantial overtreatment of clinically insignificant disease. Here, by genome-wide DNA methylome profiling, we sought to identify new biomarkers to improve the accuracy of prostate cancer diagnosis and prognosis. EXPERIMENTAL DESIGN: Eight novel candidate markers, COL4A6, CYBA, TCAF1 (FAM115A), HLF, LINC01341 (LOC149134), LRRC4, PROM1, and RHCG, were selected from Illumina Infinium HumanMethylation450 BeadChip analysis of 21 tumor (T) and 21 non-malignant (NM) prostate specimens...
January 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28035468/identification-of-a-prognostic-5-gene-expression-signature-for-gastric-cancer
#15
Jun-Yi Hou, Yu-Gang Wang, Shi-Jie Ma, Bing-Yin Yang, Qian-Ping Li
PURPOSE: Gastric cancer (GC) is a major tumor throughout the world with remaining high morbidity and mortality. The aim is to generate a gene model to assess the prognoses risk of patients with GC. METHODS: Gene expression profiling of gastric cancer patients, GSE62254 (300 samples) and GSE26253 (432 samples), was downloaded from Gene Expression Omnibus (GEO) database. Univariate survival analysis and LASSO (Least Absolute Shrinkage and Selectionator operator) (1000 iterations) of differentially expressed genes in GSE62254 was assessed using survival and glmnet in R package, respectively...
April 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28009925/synthesis-in-vitro-and-in-vivo-evaluation-of-new-hybrids-of-millepachine-and-phenstatin-as-potent-tubulin-polymerization-inhibitors
#16
Baijiao An, Shun Zhang, Jun Yan, Ling Huang, Xingshu Li
In this paper, a series of millepachine derivatives were synthesized and evaluated as tubulin polymerization inhibitors. The optimal compound 5i, (3-hydroxy-4-methoxyphenyl)(5-methoxy-2,2-dimethyl-2H-chromen-8-yl)methanone, displayed the highest cytotoxicity toward a series of cancer cells (ranging from 18 to 45 nM of IC50). Further investigation revealed that 5i significantly repressed the multidrug resistant cells (A549/CDDP, A2780/TAX) and had little cytotoxicity towards human normal cells (HLF, BJ). Cellular mechanism studies demonstrated that 5i induced G2/M phase arrest and apoptosis, which was associated with the collapse of the mitochondrial membrane potential (MMP)...
December 23, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28006662/potent-anticancer-activity-of-a-new-bismuth-iii-complex-against-human-lung-cancer-cells
#17
Ruizhuo Ouyang, Yang Yang, Xiao Tong, Kai Feng, Yaoqin Yang, Huihong Tao, Xiaoshen Zhang, Tianyu Zong, Penghui Cao, Fei Xiong, Ning Guo, Yuhao Li, Yuqing Miao, Shuang Zhou
The aim of this work is experimental study of an interesting bismuth(III) complex derived from pentadentate 2,6-pyridinedicarboxaldehyde bis((4)N-methylthiosemicarbazone), [BiL(NO3)2]NO3 {L=2,6-pyridinedicarboxaldehyde bis((4)N-methylthiosemicarbazone)}. A series of in vitro biological studies indicate that the newly prepared [BiL(NO3)2]NO3 greatly suppressed colony formation, migration and significantly induced apoptosis of human lung cancer cells A549 and H460, but did not obviously decrease the cell viability of non-cancerous human lung fibroblast (HLF) cell line, showing much higher anticancer activities than its parent ligands, especially with half maximum inhibitory concentration (IC50) <3...
December 11, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28006004/downregulation-of-tgf-%C3%AE-receptor-2-expression-and-signaling-through-inhibition-of-na-k-atpase
#18
Jennifer La, Eleanor Reed, Lan Chan, Larisa V Smolyaninova, Olga A Akomova, Gökhan M Mutlu, Sergei N Orlov, Nickolai O Dulin
Transforming growth factor-beta (TGF-β) is a multi-functional cytokine implicated in the control of cell growth and differentiation. TGF-β signals through a complex of TGF-β receptors 1 and 2 (TGFβR1 and TGFβR2) that phosphorylate and activate Smad2/3 transcription factors driving transcription of the Smad-target genes. The Na+/K+-ATPase is an integral plasma membrane protein critical for maintaining the electro-chemical gradient of Na+ and K+ in the cell. We found that inhibition of the Na+/K+ ATPase by ouabain results in a dramatic decrease in the expression of TGFβR2 in human lung fibrobalsts (HLF) at the mRNA and protein levels...
2016: PloS One
https://www.readbyqxmd.com/read/28003430/conformational-changes-in-inhibitory-pas-domain-protein-associated-with-binding-of-hif-1%C3%AE-and-bcl-xl-in-living-cells
#19
Shuya Kasai, Shinji Kajimoto, Yuma Ito, Tomo Saito, Ken-Ichi Yasumoto, Makio Tokunaga, Kumiko Sakata-Sogawa, Hiroshi Fukumura, Kazuhiro Sogawa
Inhibitory PAS domain protein (IPAS) is a dual function protein acting as a transcriptional repressor and as a pro-apoptotic protein. Simultaneous dual-color single-molecule imaging of EGFP-IPAS coexpressed with Mit-TagRFP-T in living HeLa cells revealed that fraction of EGFP-IPAS was arrested in the nucleus and on mitochondria. Transiently expressed Cerulean-IPAS in HEK293T cells was present in nuclear speckles when coexpressed with Citrine-HIF-1α or Citrine-HLF. Fluorescence lifetime imaging microscopy (FLIM) analysis of Citrine-IPAS-Cerulean in living CHO-K1 cells clarified the presence of intramolecular FRET...
December 21, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/28002927/human-lactoferrin-efficiently-targeted-into-caprine-beta-lactoglobulin-locus-with-talens
#20
Yu-Guo Yuan, Shao-Zheng Song, Meng-Ming Zhu, Zheng-Yi He, Rui Lu, Ting Zhang, Fei Mi, Jin-Yu Wang, Yong Cheng
Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with TALEN. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair (HDR). The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and conformed with PCR, and used as donor cells for somatic cell nuclear transfer (SCNT)...
December 17, 2016: Asian-Australasian Journal of Animal Sciences
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