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https://www.readbyqxmd.com/read/28728518/successful-preemptive-donor-lymphocyte-infusions-from-a-haploidentical-donor-in-a-boy-with-e2a-hlf-positive-all
#1
Maiko Hirai, Hiroshi Yagasaki, Junya Fujimura, Masami Inoue, Katsuyoshi Shimozawa, Hirotsugu Okuma, Motoaki Chin, Shori Takahashi
No abstract text is available yet for this article.
July 21, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28693249/hepatocyte-selection-medium-enriched-hepatocellular-carcinoma-cells-are-positive-for-%C3%AE-fetoprotein-and-cd44
#2
Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige
Tissues surrounding hepatocellular carcinomas (HCCs) lack glucose. Hepatocyte selection medium (HSM) is deficient in glucose and is supplemented with galactose. HCC cells were cultured in HSM to investigate the stem cell markers α-fetoprotein (AFP) and cluster of differentiation 44 (CD44). HCC cells (HLF and PLC/PRF/5 cells) were cultured in HSM. Viable cell numbers were determined on days 0 and 7 following culture in HSM. RNA was isolated and subjected to reverse transcription-quantitative PCR (RT-qPCR) to analyze the mRNA expression levels of AFP and CD44...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28685231/hybrid-polymeric-protein-nano-carriers-hppnc-for-targeted-delivery-of-tgf%C3%AE-inhibitors-to-hepatocellular-carcinoma-cells
#3
Nemany A N Hanafy, Alessandra Quarta, Riccardo Di Corato, Luciana Dini, Concetta Nobile, Vittorianna Tasco, Sonia Carallo, Mariafrancesca Cascione, Andrea Malfettone, Jitka Soukupova, Rosaria Rinaldi, Isabel Fabregat, Stefano Leporatti
TGFβ1 pathway antagonists have been considered promising therapies to attenuate TGFβ downstream signals in cancer cells. Inhibiting peptides, as P-17 in this study, are bound to either TGFβ1 or its receptors, blocking signal transduction. However, for efficient use of these TGFβ1antagonist as target therapeutic tools, improvement in their delivery is required. Here, a plasmid carrying specific shDNA (SHT-DNA), small interfering RNA (siRNA), and the peptide (P-17) were loaded separately into folic acid (FA)-functionalized nano-carriers made of Bovine Serum Albumin (BSA)...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28668848/a-novel-small-molecule-wnt-inhibitor-ic-2-has-the-potential-to-suppress-liver-cancer-stem-cells
#4
Kenzo Seto, Tomohiko Sakabe, Noriko Itaba, Junya Azumi, Hiroyuki Oka, Minoru Morimoto, Yoshihisa Umekita, Goshi Shiota
BACKGROUND/AIM: The presence of cancer stem cells (CSCs) contributes to metastasis, recurrence, and resistance to chemo/radiotherapy in hepatocellular carcinoma (HCC). The WNT signaling pathway is reportedly linked to the maintenance of stemness of CSCs. In the present study, in order to eliminate liver CSCs and improve the prognosis of patients with HCC, we explored whether small-molecule compounds targeting WNT signaling pathway suppress liver CSCs. MATERIALS AND METHODS: The screening was performed using cell proliferation assay and reporter assay...
July 2017: Anticancer Research
https://www.readbyqxmd.com/read/28663751/lactoferrin-efficiently-counteracts-the-inflammation-induced-changes-of-the-iron-homeostasis-system-in-macrophages
#5
Antimo Cutone, Luigi Rosa, Maria Stefania Lepanto, Mellani Jinnett Scotti, Francesca Berlutti, Maria Carmela Bonaccorsi di Patti, Giovanni Musci, Piera Valenti
Human lactoferrin (hLf), an 80-kDa multifunctional iron-binding cationic glycoprotein, is constitutively secreted by exocrine glands and by neutrophils during inflammation. hLf is recognized as a key element in the host immune defense system. The in vitro and in vivo experiments are carried out with bovine Lf (bLf), which shares high sequence homology and identical functions with hLf, including anti-inflammatory activity. Here, in "pure" M1 human macrophages, obtained by stimulation with a mixture of 10 pg/ml LPS and 20 ng/ml IFN-γ, as well as in a more heterogeneous macrophage population, challenged with high-dose of LPS (1 µg/ml), the effect of bLf on the expression of the main proteins involved in iron and inflammatory homeostasis, namely ferroportin (Fpn), membrane-bound ceruloplasmin (Cp), cytosolic ferritin (Ftn), transferrin receptor 1, and cytokines has been investigated...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28627280/the-targeting-effect-of-hm2e8b-nctd-liposomes-on-b-lineage-leukemia-stem-cells-is-associated-with-the-hlf-slug-axis
#6
Jingying Zhang, Diyin Shen, Min Jia, Haizhao Zhao, Yongmin Tang
To identify an agent with specific activity against B-lineage leukemia stem cells (B-LSCs), we generated norcantharidin (NCTD)-encapsulated liposomes modified with a novel humanized anti-human CD19 monoclonal antibody, Hm2E8b (Hm2E8b-NCTD-liposomes). These liposomes were specially designed to recognize and kill B-LSCs in vitro, and to decrease non-specific cytotoxicity to untargeted cells. Hm2E8b-NCTD-liposomes selectively ablated B-LSCs through targeting hepatic leukemia factor (HLF), which is implicated in hematopoietic stem cell regulation and is overexpressed in LSCs...
June 19, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28625151/na-k-atpase-as-a-target-for-treatment-of-tissue-fibrosis
#7
Sergei N Orlov, Jenifer La, Larisa V Smolyaninova, Nickolai O Dulin
Myofibroblast activation is a critical process in the pathogenesis of tissue fibrosis accounting for 45% of all deaths. No effective therapies are available for the treatment of fibrotic diseases. We focus our mini-review on recent data showing that cardiotonic steroids (CTS) that are known as potent inhibitors of Na+,K+-ATPase affect myofibroblast differentiation in a cell type-specific manner. In cultured human lung fibroblasts (HLF), epithelial cells, and cancer-associated fibroblasts, CTS blocked myofibroblast differentiation triggered by profibrotic cytokine TGF-β...
June 19, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28571757/hmgb1-promotes-hlf-1-proliferation-and-ecm-production-through-activating-hif1-%C3%AE-regulated-aerobic-glycolysis
#8
JianNing Xu, JingYing Li, ZhiHong Yu, HaiWei Rao, Shu Wang, HaiBing Lan
Aerobic glycolysis is a crucial event in fibroblast differentiation, and extracellular matrix (ECM) production in the progression of pulmonary fibrosis (PF). Abnormal high mobility group protein B1 (HMGB1) activation is involved in the pathogenesis of PF. However, whether aerobic glycolysis contributes to HMGB1-induced fibroblast proliferation and ECM production in PF has not yet been determined. In this study, we investigated the effects of HMGB1 on human embryonic lung fibroblast (HLF-1) proliferation, ECM production, and aerobic glycolysis...
May 29, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28552561/fxii-promotes-proteolytic-processing-of-the-lrp1-ectodomain
#9
Lukasz Wujak, Christina Hesse, Katherina Sewald, Danny Jonigk, Peter Braubach, Gregor Warnecke, Hans-Gerd Fieguth, Armin Braun, Günter Lochnit, Philipp Markart, Liliana Schaefer, Malgorzata Wygrecka
BACKGROUND: Factor XII (FXII) is a serine protease that is involved in activation of the intrinsic blood coagulation, the kallikrein-kinin system and the complement cascade. Although the binding of FXII to the cell surface has been demonstrated, the consequence of this event for proteolytic processing of membrane-anchored proteins has never been described. METHODS: The effect of FXII on the proteolytic processing of the low-density lipoprotein receptor-related protein 1 (LRP1) ectodomain was tested in human primary lung fibroblasts (hLF), alveolar macrophages (hAM) and in human precision cut lung slices (hPCLS)...
August 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28545024/an-unusual-intragenic-promoter-of-piwil2-contributes-to-aberrant-activation-of-oncogenic-pl2l60
#10
Shan-Shan Liu, Ning Liu, Meng-Yao Liu, Lei Sun, Wu-Yan Xia, Hong-Min Lu, Yu-Jie Fu, Guo-Liang Yang, Juan-Jie Bo, Xiao-Xing Liu, Haizhong Feng, Hailong Wu, Lin-Feng Li, Jian-Xin Gao
PIWIL2-like (PL2L) protein 60 (PL2L60), a product of aberrantly activated PIWIL2 gene, is widely expressed in various types of tumors and may promote tumorigenesis. However, the mechanisms underlying the activation of expression of PL2L60 remain unknown. In this study, an intragenic promoter responsible for the activation of PL2L60 within the human PIWIL2 gene has been identified, cloned and characterized. The promoter of PL2L60 is located in the intron 10 of the host gene PIWIL2. Bioinformatic and mutagenic analysis reveals that this intragenic promoter within the sequence of 50 nucleotides contains two closely arranged cis-acting elements specific for the hepatic leukemia factor (HLF) in the positive strand and signal transducer and activator of transcription 3 (STAT3) in the negative strand...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28503247/reconfigurable-microfluidic-device-with-discretized-sidewall
#11
Masahiro Oono, Keisuke Yamaguchi, Amirul Rasyid, Atsushi Takano, Masato Tanaka, Nobuyuki Futai
Various microfluidic features, such as traps, have been used to manipulate flows, cells, and other particles within microfluidic systems. However, these features often become undesirable in subsequent steps requiring different fluidic configurations. To meet the changing needs of various microfluidic configurations, we developed a reconfigurable microfluidic channel with movable sidewalls using mechanically discretized sidewalls of laterally aligned rectangular pins. The user can deform the channel sidewall at any time after fabrication by sliding the pins...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28498479/ppar-%C3%AE-improves-the-recovery-of-lung-function-following-acute-respiratory-distress-syndrome-by-suppressing-the-level-of-tgf-%C3%AE-1
#12
Yang Liu, Liping Xie, Mingquan Yang, Xiaofei Tan, Yonghong Zeng, Gang Zheng, Youying Chen, Ping Chen
Although peroxisome proliferator-activated receptor (PPAR)-α has been reported to be involved in preventing acute lung injury (ALI), the molecular regulation of post‑ALI lung recovery remains to be fully elucidated. The aim of the present study was to characterize the mechanism by which PPAR‑α prevents ALI and examine the role of PPAR‑α in the recovery of lung function following acute respiratory distress syndrome (ARDS). Reverse transcription‑quantitative‑polymerase chain reaction and western blot analyses suggested that PPAR‑α was effective in suppressing transforming growth factor (TGF)‑β1 in HLF cells and RAW 264...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28475629/purification-and-characterization-of-recombinant-human-bile-salt-stimulated-lipase-expressed-in-milk-of-transgenic-cloned-cows
#13
Yuhang Wang, Fangrong Ding, Tao Wang, Wenjie Liu, Susanne Lindquist, Olle Hernell, Jianwu Wang, Jing Li, Ling Li, Yaofeng Zhao, Yunping Dai, Ning Li
Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for gestational age preterm infants. Thus, purified recombinant human BSSL (rhBSSL) can be used for treatment of patients with fat malabsorption and expressing rhBSSL in the milk of transgenic cloned cows would therefore be a mean to meet a medical need...
2017: PloS One
https://www.readbyqxmd.com/read/28460412/mir-615-3p-inhibits-the-osteogenic-differentiation-of-human-lumbar-ligamentum-flavum-cells-via-suppression-of-osteogenic-regulators-gdf5-and-foxo1
#14
Jichao Yin, Guihua Zhuang, Yi Zhu, Xinglv Hu, Hongmou Zhao, Rongqiang Zhang, Hao Guo, Xiaochen Fan, Yi Cao
Ossification of the ligamentum flavum (OLF) is a disease of heterotopic ossification in spinal ligaments. The key of the OLF pathogenesis is the differentiation of fibroblasts into osteoblasts. In this study, we explored the role of miR-615-3p in the osteogenic differentiation of human LF cells. The expression of miR-615-3p was detected during the osteogenic differentiation of hFOB1.19 human osteoblasts, human BMSCs, and human LF cells. The qPCR results showed that miR-615-3p was being decreased during the osteogenic differentiation of these cell lineages...
July 2017: Cell Biology International
https://www.readbyqxmd.com/read/28454244/proliferation-of-sphere-forming-hepatocellular-carcinoma-cells-is-suppressed-in-a-medium-without-glucose-and-arginine-but-with-galactose-and-ornithine
#15
Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige
Resistance to sorafenib in hepatocellular carcinoma (HCC) cells exhibiting stemness was evaluated using a sphere formation assay. A hepatocyte selection medium (HSM) deficient in glucose and arginine was used to suppress the proliferation of cell spheres composed of HLF and PLC/PRF/5 HCC cells, which were subjected to a sphere formation assay. Cell spheres were cultured with sorafenib and subjected to a cell proliferation assay and the expression levels of cytochrome P450 (CYP3A4) were analyzed in RNA extracted from sphere-forming cells using reverse transcription-quantitative polymerase chain reaction...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28407300/thrombin-induces-ccl2-expression-in-human-lung-fibroblasts-via-p300-mediated-histone-acetylation-and-nf-kappab-activation
#16
Xiaoling Deng, Xiaoqiong Zhou, Yan Deng, Fan Liu, Xiaofan Feng, Qi Yin, Yinzhen Gu, Songlin Shi, Mingyan Xu
Thrombin has been shown to play a key role in lung diseases such as pulmonary fibrosis via the induction of fibrotic cytokine- chemokine (CC motif) ligand-2 (CCL2) expression. We previously reported that transcription factor nuclear factor-κB (NF-κB) is responsible for thrombin-induced CCL2 expression in human lung fibroblasts (HLFs). Here, we extended our study to investigate the epigenetic regulation mechanism for thrombin-induced CCL2 expression in HLFs. HLFs were cultured in F-12 medium. CCL2 protein and mRNA levels were detected by ELISA and quantitative real-time PCR, respectively...
April 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28402947/overexpression-of-the-transcription-factor-atf3-with-a-regulatory-molecular-signature-associates-with-the-pathogenic-development-of-colorectal-cancer
#17
Feng Yan, Le Ying, Xiaofang Li, Bin Qiao, Qiaohong Meng, Liang Yu, Xiangliang Yuan, Shu-Ting Ren, David W Chan, Liyun Shi, Peihua Ni, Xuefeng Wang, Dakang Xu, Yiqun Hu
The identification of novel biomarkers of cancer is important for improved diagnosis and prognosis. With an abundant amount of resources in the publicly available database, such as the Cancer Genome Atlas (TCGA) database, an integrative strategy is used to systematically characterize the aberrant patterns of colorectal cancer (CRC) based on RNA-Seq, chromatin immunoprecipitation sequencing (ChIP-Seq), tissue microarray (TMA), gene profiling and molecular signatures. The expression of the transcription factor ATF3 was elevated in human CRC specimens in a TMA by immunochemistry analysis compared to the adjacent normal tissues...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28356961/2-deoxyglucose-and-sorafenib-synergistically-suppress-the-proliferation-and-motility-of-hepatocellular-carcinoma-cells
#18
Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige
Cancer cells consume more glucose than normal cells, mainly due to their increased rate of glycolysis. 2-Deoxy-d-glucose (2DG) is an analogue of glucose, and sorafenib is a kinase inhibitor and molecular agent used to treat hepatocellular carcinoma (HCC). The present study aimed to demonstrate whether combining 2DG and sorafenib suppresses tumor cell proliferation and motility more effectively than either drug alone. HLF and PLC/PRF/5 HCC cells were incubated with sorafenib with or without 1 µM 2DG, and subjected to a proliferation assay...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28257520/lactoferrin-binding-protein-b-a-bi-functional-bacterial-receptor-protein
#19
Nicholas K H Ostan, Rong-Hua Yu, Dixon Ng, Christine Chieh-Lin Lai, Anastassia K Pogoutse, Vladimir Sarpe, Morgan Hepburn, Joey Sheff, Shaunak Raval, David C Schriemer, Trevor F Moraes, Anthony B Schryvers
Lactoferrin binding protein B (LbpB) is a bi-lobed outer membrane-bound lipoprotein that comprises part of the lactoferrin (Lf) receptor complex in Neisseria meningitidis and other Gram-negative pathogens. Recent studies have demonstrated that LbpB plays a role in protecting the bacteria from cationic antimicrobial peptides due to large regions rich in anionic residues in the C-terminal lobe. Relative to its homolog, transferrin-binding protein B (TbpB), there currently is little evidence for its role in iron acquisition and relatively little structural and biophysical information on its interaction with Lf...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28208261/construction-of-aiegens-based-bioprobe-with-two-fluorescent-signals-for-enhanced-monitor-of-extracellular-and-intracellular-telomerase-activity
#20
Yuan Zhuang, Chunli Shang, Xiaoding Lou, Fan Xia
Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of telomerase, two positively charged AIEgens (Silole-R and TPE-H) bind to quencher group labeled primer (QP) and the extension repeated units, leading enhancement of two telomerase-triggered fluorescent signals. Furthermore, by combination the wider linear range in vitro and lower background in living cells imaging, the bioprobe is used to detect telomerase extracted from various cell lines (MCF-7, HeLa, E-J, and HLF), 50 bladder cancer patients' urine samples, 10 normal people's urine samples, and also applied in mapping telomerase activity inside living cells (MCF-7, HeLa, MDA-MB-231, and HT1080)...
February 7, 2017: Analytical Chemistry
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