keyword
MENU ▼
Read by QxMD icon Read
search

Marek's disease virus

keyword
https://www.readbyqxmd.com/read/28548038/marek-s-disease-virus-infection-of-phagocytes-a-de-novo-in-vitro-infection-model
#1
Pankaj Chakraborty, Lonneke Vervelde, Robert G Dalziel, Peter S Wasson, Venugopal Nair, Bernadette M Dutia, Pete Kaiser
Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells...
May 26, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28532188/differential-amplification-of-marek-s-disease-cvi988-vaccine-and-of-wild-type-isolates-from-organs-of-commercial-chickens-using-single-or-duplexed-probes-in-real-time-pcr
#2
Irit Davidson, Amira Natour-Altoury, Israel Raibstein, Yaad Dahan
The DIVA test, based on the MDV pp38 gene was described by Baigent et al. (2016), using similar primers and alternate probes for virulent MDV-1 and the vaccine CVI988 virus. We now explored the assay applicability for commercial vaccines and for DNAs of commercial chickens, as former study employed tissue cultured prototype MDV strains and chicken tissue from experimental infection trials. DNA of chicken visceral organs and feathers of either vaccinated, or naturally-infected with virulent MDV-1 wild-type viruses was used for DIVA-PCR real-time PCR...
May 22, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28528445/interindividual-spread-of-herpesviruses
#3
Keith W Jarosinski
Interindividual spread of herpesviruses is essential for the virus life cycle and maintenance in host populations. For most herpesviruses, the virus-host relationship is close, having coevolved over millions of years resulting in comparatively high species specificity. The mechanisms governing interindividual spread or horizontal transmission are very complex, involving conserved herpesviral and cellular proteins during the attachment, entry, replication, and egress processes of infection. Also likely, specific herpesviruses have evolved unique viral and cellular interactions during cospeciation that are dependent on their relationship...
2017: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/28510513/putative-roles-as-oncogene-or-tumour-suppressor-of-the-mid-clustered-micrornas-in-gallid-alphaherpesvirus-2-gahv2-induced-marek-s-disease-lymphomagenesis
#4
Man Teng, Zu-Hua Yu, Pu Zhao, Guo-Qing Zhuang, Zi-Xiang Wu, Lu Dang, Hui-Zhen Li, Sheng-Ming Ma, Zhi-Zhong Cui, Gai-Ping Zhang, Run Wu, Jun Luo
In the last decade, numerous microRNAs (miRNAs) have been identified in diverse virus families, particularly in herpesviruses. Gallid alphaherpesvirus 2 (GaHV2) is a representative oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts, namely Marek's disease (MD). In the GaHV2 genome there are 26 mature miRNAs derived from 14 precursors assembled into three clusters, namely the Meq-cluster, Mid-cluster and LAT-cluster. Several GaHV2 miRNAs, especially those in the Meq-cluster (e...
May 15, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28475033/differentially-expressed-genes-during-spontaneous-lytic-switch-of-marek-s-disease-virus-in-lymphoblastoid-cell-lines-determined-by-global-gene-expression-profiling
#5
William N Mwangi, Deepali Vasoya, Lydia B Kgosana, Mick Watson, Venugopal Nair
Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes Marek's disease and is characterized by visceral CD4+TCRαβ+ T-cell lymphomas in susceptible hosts. Immortal cell lines harbouring the viral genome have been generated from ex vivo cultures of MD tumours. As readily available sources of large numbers of cells, MDV-transformed lymphoblastoid cell lines (LCLs) are extremely valuable for studies of virus-host interaction. While the viral genome in most cells is held in a latent state, minor populations of cells display spontaneous reactivation identifiable by the expression of lytic viral genes...
April 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28414218/exploiting-the-anti-aggregation-of-gold-nanostars-for-rapid-detection-of-hand-foot-and-mouth-disease-causing-enterovirus-71-using-surface-enhanced-raman-spectroscopy
#6
Miguel Reyes, Marek Piotrowski, Swee Kim Ang, Jingqi Chan, Shuai He, Justin Jang Hann Chu, James Chen Yong Kah
Enterovirus 71 (EV71) is a major public health threat that requires rapid point-of-care detection. Here, we developed a surface-enhanced Raman spectroscopy (SERS)-based scheme that utilized protein-induced aggregation of colloidal gold nanostars (AuNS) to rapidly detect EV71 without the need for fabricating a solid substrate, Raman labels or complicated sample handling. We used AuNS (hydrodynamic diameter, DH of 105.12 ± 1.13 nm) conjugated to recombinant scavenger receptor class B, member 2 (SCARB2) protein with known affinity to EV71...
April 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28413051/protective-efficacy-of-a-novel-recombinant-marek-s-disease-virus-vector-vaccine-against-infectious-bursal-disease-in-chickens-with-or-without-maternal-antibodies
#7
Kai Li, Yongzhen Liu, Yanping Zhang, Li Gao, Changjun Liu, Hongyu Cui, Xiaole Qi, Yulong Gao, Li Zhong, Xiaomei Wang
Infectious bursal disease (IBD) causes significant clinical and economic losses to the poultry industry worldwide. Current vaccine programs using live attenuated and inactivated vaccines have numerous drawbacks. As an alternative solution to control IBD, a Marek's disease virus (MDV) vector vaccine (rMDV-VP2) expressing the VP2 gene of infectious bursal disease virus (IBDV) has been developed. In this study, the protective efficacy of rMDV-VP2 was evaluated in a dose-related experiment which showed that a single dose of 1000 PFU was sufficient to fully protect chickens against IBDV infection...
April 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28410454/transcriptomic-analysis-of-host-immune-response-in-the-skin-of-chickens-infected-with-marek-s-disease-virus
#8
Mohammad Heidari, Phillip C Delekta
Marek's disease virus (MDV), a highly cell-associated oncogenic α-herpesvirus, is the causative agent of T cell lymphoma and neuropathic disease called Marek's disease. The skin is the only anatomical site where infectious enveloped cell-free virions are produced and shed into the environment. Studies have demonstrated that MDV infection induces immunological responses within the skin, including the release of cytokines and the recruitment of T lymphocytes. The host immune response, however, is not sufficient to block replication and shedding of the virus particles from the skin...
April 14, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28406989/escdl-1-a-new-cell-line-derived-from-chicken-embryonic-stem-cells-supports-efficient-replication-of-mardiviruses
#9
Jean-François Vautherot, Christian Jean, Laetitia Fragnet-Trapp, Sylvie Rémy, Danièle Chabanne-Vautherot, Guillaume Montillet, Aurélie Fuet, Caroline Denesvre, Bertrand Pain
Marek's disease virus is the etiological agent of a major lymphoproliferative disorder in poultry and the prototype of the Mardivirus genus. Primary avian somatic cells are currently used for virus replication and vaccine production, but they are largely refractory to any genetic modification compatible with the preservation of intact viral susceptibility. We explored the concept of induction of viral replication permissiveness in an established pluripotent chicken embryonic stem cell-line (cES) in order to derive a new fully susceptible cell-line...
2017: PloS One
https://www.readbyqxmd.com/read/28381111/spontaneous-spinal-plexiform-perineurioma-in-a-juvenile-chicken-gallus-domesticus
#10
Lara Cusack, Uriel Blas-Machado, Joerg Mayer
A 2-mo-old pet chicken ( Gallus domesticus) was presented because of lameness and a hind limb mass of 1 mo duration. Radiographs revealed a soft tissue mass extending from the mid-femur into the body wall. Cytology of a sample obtained from a fine-needle aspirate was unremarkable. The bird was prepared for surgical investigation of the mass but died acutely at the time of pre-medication. Autopsy revealed an extradural mesenchymal neoplasm that focally compressed the lumbar spinal cord, extended into and along the adjacent nerve structures, and displaced the kidneys, skeletal muscle, and body wall...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28368367/a-chinese-variant-marek-s-disease-virus-strain-with-divergence-between-virulence-and-vaccine-resistance
#11
Guo-Rong Sun, Yan-Ping Zhang, Hong-Chao Lv, Lin-Yi Zhou, Hong-Yu Cui, Yu-Long Gao, Xiao-le Qi, Yong-Qiang Wang, Kai Li, Li Gao, Qing Pan, Xiao-Mei Wang, Chang-Jun Liu
Marek's disease (MD) virus (MDV) has been evolving continuously, leading to increasing vaccination failure. Here, the MDV field strain BS/15 was isolated from a severely diseased Chinese chicken flock previously vaccinated with CVI988. To explore the causes of vaccination failure, specific-pathogen free (SPF) chickens vaccinated with CVI988 or 814 and unvaccinated controls were challenged with either BS/15 or the reference strain Md5. Both strains induced MD lesions in unvaccinated chickens with similar mortality rates of 85...
April 3, 2017: Viruses
https://www.readbyqxmd.com/read/28346793/early-immune-responses-to-marek-s-disease-vaccines
#12
Mohammad Heidari, Dan Wang, Shuhong Sun
Marek's disease virus (MDV), a highly cell-associated lymphotropic α-herpesvirus, is the causative agent of Marek's disease (MD) in domestic chickens. MDV replicates in chicken cells and establishes a latent infection within CD4(+) T cells. Although MD vaccines have been in use for several decades, the exact mechanism of vaccine-induced protection is unclear. It is believed that the innate immune system plays a role in vaccine-induced immunity against pathogenic strains of MDV. To shed light on the possible function of the innate immunity in vaccine-mediated protection, we investigated the effect of vaccination, Rispens/CVI988, on the activation of cellular components of the innate immune system by analyzing the expression pattern of select immune-related genes in the cecal tonsils (CT) and duodenum of two MD-susceptible and MD-resistant chicken lines at 3, 5, and 10 days postvaccination (dpv)...
April 2017: Viral Immunology
https://www.readbyqxmd.com/read/28339787/molecular-characterization-of-marek-s-disease-virus-in-a-poultry-layer-farm-from-colombia
#13
Sara López-Osorio, Diego Piedrahita, Maria A Espinal-Restrepo, Gloria C Ramírez-Nieto, Venugopal Nair, Susan M Williams, Susan Baigent, César Ventura-Polite, Diego A Aranzazu-Taborda, Jenny J Chaparro-Gutiérrez
Marek's disease ( ) is a lymphoproliferative disease caused by an Alphaherpesvirus, genus Mardivirus , serotype 1 ( Gallid Herpesvirus 2 , GaHV-2) that includes all known pathogenic strains. In addition to Marek's disease virus ( ) serotype 1, the genus includes 2 distinct nonpathogenic serotypes: serotype 2 (GaHV-3) and serotype 3 ( Meleagridis Herpesvirus 1 , MeHV-1) which are used in commercially available vaccines against MD. As a result of vaccination, clinical signs are not commonly observed, and new cases are usually associated with emerging variant strains against which the vaccines are less effective...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28301231/evaluation-and-identification-of-marek-s-disease-virus-bac-clones-as-standardized-reagents-for-research
#14
John R Dunn, Sanjay M Reddy, Masahiro Niikura, Venugopal Nair, Janet E Fulton, Hans H Cheng
Marek's disease virus (MDV) is an alphaherpesvirus that causes Marek's disease (MD), a lymphoproliferative disease in chickens. Understanding of MDV gene function advanced significantly following the cloning of the MDV genome as either a series of overlapping cosmids or as a bacterial artificial chromosome (BAC), both of which could produce viable MDV. The objectives of this study were to compare multiple virulent MDV BAC clones using the Avian Disease and Oncology Laboratory's pathotyping assay, and to demonstrate the use of these clones as standardized reagents for a modified pathotyping assay by other laboratories...
March 2017: Avian Diseases
https://www.readbyqxmd.com/read/28283855/the-role-of-human-parainfluenza-virus-infections-in-the-immunopathology-of-the-respiratory-tract
#15
REVIEW
Malgorzata Pawełczyk, Marek Leszek Kowalski
Viral infections are leading causes of both upper and lower airway acute illness in all age groups of healthy persons, and have also been implicated in the acute exacerbations of chronic respiratory disorders like asthma and COPD. Human rhinovirus, respiratory syncytial virus, influenza virus and coronavirus have been considered as the most important respiratory pathogens and relatively little attention has been paid to the role of parainfluenza viruses (hPIVs). Human parainfluenza viruses are single-stranded RNA viruses belonging to the paramyxovirus family that may evoke lower respiratory infections in infants, children and immunocompromised individuals...
March 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28263843/comparison-of-different-sites-in-recombinant-marek-s-disease-virus-for-the-expression-of-green-fluorescent-protein
#16
Yongzhen Liu, Kai Li, Hongyu Cui, Li Gao, Changjun Liu, Yanping Zhang, Yulong Gao, Xiaomei Wang
Marek's disease virus (MDV) is a preferred vector for recombinant vaccine construction, and insertion site is the main factor influencing foreign gene expression and vaccine efficacy. In this study, recombinant MDVs inserted with the enhanced green fluorescent protein (eGFP) gene at different sites in its genome were generated from overlapping fosmid DNAs and the eGFP expression was compared. The results showed that the eGFP expression levels from the sites in the unique long (UL) region (within UL41, between UL45 and UL46, and between UL55 and LORF10) were comparable, which were significantly higher than those from the sites in the unique short (US) region (US2 and US10), and the eGFP expression level from US2 was significantly higher than that from US10...
March 2, 2017: Virus Research
https://www.readbyqxmd.com/read/28194622/characterization-of-a-gallid-herpesvirus-2-strain-with-novel-reticuloendotheliosis-virus-long-terminal-repeat-inserts
#17
Yan-Ping Zhang, Ke-Yan Bao, Guo-Rong Sun, Hong-Chao Lv, Hong-Yu Cui, Yu-Long Gao, Xiao-Mei Wang, Chang-Jun Liu
A bacterial artificial chromosome clone, designated LCY, was constructed from a Gallid herpesvirus 2 (GaHV-2) isolate from a GaHV-2 and reticuloendotheliosis virus co-infected clinical sample. The LCY GaHV-2 insert was sequenced and found to consist of 175,319 nucleotides. LCY GaHV-2 open reading frames (ORFs) had a high sequence identity to those of reference strains. The major difference was that two REV long terminal repeats (LTRs), in the same direction, were inserted at the internal repeat short (IRs)/unique short (Us) and Us/terminal repeat short (TRs) junctions...
June 2017: Virus Genes
https://www.readbyqxmd.com/read/28160668/expression-of-marek-s-disease-virus-oncoprotein-meq-during-infection-in-the-natural-host
#18
S-H Sheldon Tai, Cari Hearn, Sudawapee Umthong, Olga Agafitei, Hans H Cheng, John R Dunn, Masahiro Niikura
Gallid herpesvirus 2 (Marek's disease virus, MDV) causes lymphoproliferative Marek's disease (MD), and is unique among alphaherpesviruses as the viral genome encodes an oncoprotein, Meq. To elucidate the temporal relationship between Meq expression and the development of MD lymphomas in infected chickens, we generated a virulent recombinant MDV that expresses GFP simultaneously with Meq. By using this virus, we monitored the dynamics of Meq expression in vivo throughout the course of infection. In peripheral blood mononuclear cells, the percentage of Meq-expressing cells dramatically increased in the early latent phase but decreased thereafter...
March 2017: Virology
https://www.readbyqxmd.com/read/28160143/molecular-characterisation-of-fowl-adenovirus-type-7-isolated-from-poultry-associated-with-inclusion-body-hepatitis-in-poland
#19
Jowita Samanta Niczyporuk
The fowl adenovirus field strain FAdV-JSN-5/10j (GenBank accession number KP879219) was isolated from the intestine of a 7-week-old chicken diagnosed with inclusion body hepatitis and simultaneously with Marek's disease, and for that reason, it was chosen for molecular study. It was identified as fowl adenovirus genotype 7 (species Fowl aviadenovirus E) based on nucleotide sequence analysis of the loop L1 region of the hexon gene. Nucleotide sequence alignment of this strain, FAdV-7 reference strains B-3A ATCC VR-832 (AF339922) and YR36 (AF508955), and eight additional FAdV-7 field strains confirmed its classification as FAdV-JS-5/10j and showed that these viruses are very similar to each other...
May 2017: Archives of Virology
https://www.readbyqxmd.com/read/28151004/evaluation-of-factors-influencing-the-development-of-late-marek-s-disease-virus-induced-immunosuppression-virus-pathotype-and-host-sex
#20
Nik M Faiz, Aneg L Cortes, James S Guy, Oscar J Fletcher, Thomas Cimino, Isabel M Gimeno
Marek's disease virus (MDV) is a herpesvirus that induces lymphoma and immunosuppression in chickens. MDV-induced immunosuppression (MDV-IS) is complex and can be divided into two phases: early-MDV-IS associated with cytolytic infection in the lymphoid organs in chickens lacking maternal antibodies against MDV (MAbs) and late-MDV-IS that appears later in the pathogenesis and occurs even in chickens bearing MAbs. We have recently developed a model to reproduce late-MDV-IS under laboratory conditions. This model evaluates late-MDV-IS indirectly by assessing the effect of MDV infection on the efficacy of infectious laryngotracheitis (ILT) vaccines against challenge with ILT virus...
April 24, 2017: Avian Pathology: Journal of the W.V.P.A
keyword
keyword
774
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"