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enterococus faecalis

Chieu D Tran, Franja Prosenc, Mladen Franko, Gerald Benzi
A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag(+)) or Ag(0) nanoparticles (Ag(+)NPs or Ag(0)NPs). In this method, butylmethylimmidazolium chloride ([BMIm(+)Cl(-)]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm(+)Cl(-)] solution of [CEL+KER] during the dissolution process. The silver in the composites can be maintained as ionic silver (Ag(+)) or completely converted to metallic silver (Ag(0)) by reducing it with NaBH4...
December 21, 2016: ACS Applied Materials & Interfaces
Haihong Hao, Shengxi Zhou, Guyue Cheng, Menghong Dai, Xu Wang, Zhenli Liu, Yulian Wang, Zonghui Yuan
To evaluate microbiological safety of tulathromycin on human intestinal bacteria, tulathromycin (0, 0.1, 1, 10, and 100 μg/mL) was added into Chemostats. Before and after drug exposure, we monitored (1) population, SCFA products, antimicrobial resistance, and colonization resistance of gut microbiota, and (2) the antimicrobial resistance genes, transferability, virulent genes, pathogenicity of Enterococus faecalis. Results showed that low level of tulathromycin did not exhibit microbiological hazard on resistance selection and colonization resistance...
2016: Frontiers in Microbiology
Daiana Elisabeth Böttcher, Nicole T Sehnem, Francisco Montagner, Clarissa Cavalcanti Fatturi Parolo, Fabiana Soares Grecca
INTRODUCTION: The aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (CHX) solution on dentin by means of confocal laser scanning microscopy and high-performance liquid chromatography for 48 hours, 7 days, and 30 days. METHODS: One hundred twenty-three extracted human teeth were used. Samples were divided into 4 groups according to the solution (CHX or saline) and the presence of Enterococus faecalis biofilm. Samples were kept in contact with 5 mL of the solution for 5 minutes...
August 2015: Journal of Endodontics
Esmeray Kuley, Fatih Ozogul, Yesim Ozogul, Ismail Akyol
The influences of lactic acid bacteria and brine solutions on the biogenic amine formation by Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococus faecalis, Pseudomonas aeruginosa, Listeria monocytogenes, Aeromonas hydrophila and Salmonella paratyphi A in fermented trout fillets were investigated. Fish fillets were divided into four groups, group 1 without any lactic acid bacteria inoculation, group 2 and group 3 with different salt concentration inoculated with lactic acid bacteria and food-borne pathogens, and group 4 inoculated with lactic acid bacteria and food-borne pathogens without a salt solution...
December 1, 2011: Food Chemistry
Galina Stoyancheva, Marta Marzotto, Franco Dellaglio, Sandra Torriani
The human vagina is a complex and dynamic ecosystem containing an abundance of microorganisms. In women of childbearing age, this system is dominated by Lactobacillus spp. In the present work, seventeen newly isolated vaginal strains were identified by 16S rDNA sequencing and were investigated for their antimicrobial properties. Twelve of the isolated Lactobacillus strains showed activity against one or more microorganisms. Six and five of them produced substances that inhibited the growth of two different Klebsiella strains and Staphylococcus aureus, respectively...
September 2014: Archives of Microbiology
Ana Del Olmo, Javier Calzada, Manuel Nuñez
The bactericidal activity of lactoferrin (LF), amidated lactoferrin (AMILF), pepsin digested lactoferrin (PDLF), and its activated (ALF) commercial form, against six strains of three gram-positive bacterial species was investigated. Listeria monocytogenes was most sensitive in vitro, Staphylococcus aureus showed a moderate resistance, and Enterococus faecalis was highly resistant to antimicrobials. When chicken breast fillets were inoculated with L. monocytogenes CECT5725 and treated with antimicrobials, reductions were below 0...
January 2012: Meat Science
K Hegstad, T Mikalsen, T M Coque, G Werner, A Sundsfjord
Abstract Mobile genetic elements (MGEs) including plasmids and transposons are pivotal in the dissemination and persistence of antimicrobial resistance in Enterococcus faecalis and E. faecium. Enterococcal MGEs have also been shown to be able to transfer resistance determinants to more pathogenic bacteria such as Staphylococcus aureus. Despite their importance, we have a limited knowledge about the prevalence, distribution, and genetic content of specific MGEs in enterococcal populations. Molecular epidemiological studies of enterococcal MGEs have been hampered by the lack of standardized molecular typing methods and relevant genome information...
March 23, 2010: Clinical Microbiology and Infection
Antonio Sánchez Valenzuela, Nabil Ben Omar, Hikmate Abriouel, Rosario Lucas López, Elena Ortega, Magdalena Martínez Cañamero, Antonio Gálvez
A collection of enterococci isolated from meat, dairy and vegetable foods from Morocco including 23 Enterococus faecalis and 15 Enterococcus faecium isolates was studied. All isolates were sensitive to ampicillin, penicillin, and gentamicin. Many E. faecalis isolates were resistant to tetracycline (86.95%), followed by rifampicin (78.26% ciprofloxacin (60.87%), quinupristin/dalfopristin (56.52%), nitrofurantoin (43.47%), levofloxacin (39.13%), erythromycin (21.73%), streptomycin (17.39%), chloramphenicol (8...
August 2008: Food and Chemical Toxicology
Eugenia Gospodarek, Beata Ulatowska, Jolanta Powierska-Czarny, Jan Talar, Małgorzata Lukowicz
Background. The purpose of our study was to evaluate the occurrence of Gram-positive microorganisms isolated from patients hospitalized in the Department and Clinic of Rehabilitation at the Rydygier Medical University in Bydgoszcz. Materials and methods. The material analyzed consisted of 533 clinical samples collected from patients hospitalized in 2000-2001. The study included 485 Gram-positive bacterial strains isolated from clinical material. Morphological characteristics provided the basis for the identification of bacteria...
January 31, 2002: Ortopedia, Traumatologia, Rehabilitacja
Karthe Ponnuraj, Sthanam V L Narayana
No abstract text is available yet for this article.
October 1, 2007: Proteins
Alain Rincé, Yoann Le Breton, Nicolas Verneuil, Jean-Christophe Giard, Axel Hartke, Yanick Auffray
Analysis of the susceptibility and the acquisition of tolerance in Enterococus faecalis towards bile salts showed a nearly instantaneous killing effect and yielded evidence for homologous tolerance as well as for cross-protections. Two-dimensional (2-D) electrophoresis revealed 45 proteins which are amplified in response to the bile salt treatment. These include a set of seven proteins, the synthesis of which is increased not only with the bile salts but also with multiple sublethal stresses of various nature...
December 1, 2003: International Journal of Food Microbiology
Elizaveta A Permina, Andrey A Mironov, Mikhail S Gelfand
It is known that umuDC-like operons encoding DNA polymerase V are often found in plasmids of gamma-proteobacteria. Here we demonstrate that homologous operons are associated with mobile genomic elements in Gram-positive bacteria as well. Using the comparative analysis of transcriptional regulatory signals, we suggest that genes encoding homologs of UmuC in prophages of Bacillus subtilis and transposons of Enterococus faecalis are regulated by DinR (SOS repressor). We also predict a functional link between the polymerase V-like proteins of B...
June 26, 2002: Gene
Y Higurashi, K Okuzumi, A Yoneyama, K Nakahara
The in vitro antibacterial activity of cefozopran (CZOP) against recent clinical isolates was evaluated and compared with those of ceftazidime (CAZ), cefpirome (CPR), cefepime (CFPM), cefotaxime (CTX), sulbactam/cefoperazone (S/C), imipenem (IPM), oxacillin (MPIPC), and flomoxef (FMOX). MIC80 values of CZOP for methicillin-susceptible Staphylococcus aureus (MSSA, n = 41), methicillin-resistant Staphylococcus aureus (MRSA, n = 57), Streptococcus pneumoniae (n = 45), Enterococus faecalis (n = 49), Enterobacter cloacae (n = 50), Citrobacter freundii (n = 45), Serratia marcescens (n = 45), and Pseudomonas aeruginosa (n = 100) were 1, 32, 2, 16, 4, 1, 0...
December 1997: Japanese Journal of Antibiotics
J Nakayama, S Igarashi, H Nagasawa, D B Clewell, F Y An, A Suzuki
Enterococus faecalis plasmid pAM373 encodes a mating response to the sex pheromone, cAM373, which is secreted from pAM373-free E. faecalis. cAM373-like activity was detected in a culture filtrate of Staphylococcus aureus. The major active substance, termed staph-cAM373, was isolated, and its structure was identified as a H-Ala-IIe-Phe-IIe-Leu-Ala-Ala-OH heptapeptide.
June 1996: Bioscience, Biotechnology, and Biochemistry
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