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https://www.readbyqxmd.com/read/29667535/clinical-features-and-short-term-outcome-of-presumptive-intracranial-complications-associated-with-otitis-media-interna-a-multi-center-retrospective-study-of-19-cats-2009-2017
#1
Sarah A Moore, R Timothy Bentley, Sheila Carrera-Justiz, Kari D Foss, Ronaldo C da Costa, Laurie B Cook
Objectives The aim of this study was to compile an overview of the clinical features of intracranial complication of otitis media/interna (OMI) in cats managed across five veterinary referral hospitals. Of additional interest were culture results that could inform empirical antibiotic selection, as well as outcome with both medical and surgical management. Methods A retrospective medical record review was conducted at five veterinary referral practices to identify cats with a diagnosis of intracranial complication secondary to OMI between 2009 and 2017...
April 1, 2018: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/29667111/propofol-specifically-suppresses-il-1%C3%AE-secretion-but-increases-bacterial-survival-in-staphylococcus-aureus-infected-raw264-7-cells
#2
Ming-Shan Chen, Wen-Chun Lin, Hsuan-Te Yeh, Chia-Lin Hu, Shew-Meei Sheu
Anesthetics have immunomodulatory effects, but the use of different assay systems has contributed to inconsistent results in the literature. IL-1β and reactive oxygen species (ROS) secreted by phagocytes are important factors that protect against Staphylococcus aureus infection. In this study, the effects of four intravenous anesthetics (propofol, thiamylal sodium, midazolam, and ketamine) on IL-1β secretion, ROS, and bacterial survival in S. aureus-infected RAW264.7 cells were evaluated. S. aureus-infected RAW264...
April 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29666540/antimicrobial-antioxidant-and-probiotics-characterization-of-dominant-bacterial-isolates-from-traditional-fermented-fish-of-manipur-north-east-india
#3
Sambanduram Samarjit Singh, Surajit De Mandal, Esther Lalnunmawii, Nachimuthu Senthil Kumar
Utonga - kupsu , Hentak and Ngari are traditional fermented fish products produced by the Manipuri people living in the North-Eastern part of India. The present study was designed with the aim to isolate, identify and characterize the microorganisms present in these fermented foods. Bacterial pure cultures were isolated using serially diluted samples and were further identified by conventional biochemical tests and Sanger sequencing of 16s rRNA gene. Results show that the number of bacterial count in Nutrient agar and Starch casein agar was 14-20 and 10-16 CFU/g, respectively...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29666286/viable-but-nonculturable-listeria-monocytogenes-and-salmonella-enterica-serovar-thompson-induced-by-chlorine-stress-remain-infectious
#4
Callum J Highmore, Jennifer C Warner, Steve D Rothwell, Sandra A Wilks, C William Keevil
The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29666284/biofilms-comprise-a-component-of-the-annual-cycle-of-vibrio-cholerae-in-the-bay-of-bengal-estuary
#5
Marzia Sultana, Suraia Nusrin, Nur A Hasan, Abdus Sadique, Kabir U Ahmed, Atiqul Islam, Anwar Hossain, Ira Longini, Azhar Nizam, Anwar Huq, Abul K Siddique, David A Sack, Richard B Sack, Rita R Colwell, Munirul Alam
Vibrio cholerae , an estuarine bacterium, is the causative agent of cholera, a severe diarrheal disease that demonstrates seasonal incidence in Bangladesh. In an extensive study of V. cholerae occurrence in a natural aquatic environment, water and plankton samples were collected biweekly between December 2005 and November 2006 from Mathbaria, an estuarine village of Bangladesh near the mangrove forests of the Sundarbans. Toxigenic V. cholerae exhibited two seasonal growth peaks, one in spring (March to May) and another in autumn (September to November), corresponding to the two annual seasonal outbreaks of cholera in this region...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29666193/lmo1656-is-a-secreted-virulence-factor-of-listeria-monocytogenes-that-interacts-with-the-sortin-nexin-6-bar-complex
#6
Daryl Jason David, Alessandro Pagliuso, Lilliana Radoshevich, Marie-Anne Nahori, Pascale Cossart
Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29665574/diagnostic-methods-to-detect-uterus-illnesses-in-mares
#7
Miguel Alejandro Silva Rua, Celia Raquel Quirino, Rachel Bittencourt Ribeiro, Eulogio Carlos Queiroz Carvalho, Maria de Lourdes Amaral Bernadino, Aylton Bartholazzi Junior, Luisa Fernanda Cipagalta, Marcus Antônio Pessanha Barreto
Uterine illnesses are the major problem in horse reproductive programs. The diagnosis of these pathologies is often neglected or does not provide enough information about the cause. The aim of this study was to evaluate different diagnoses to provide more accurate information about endometritis and the real endometrial profile at the beginning of a breeding season. Fifty-one mares with reproductive failure were evaluated. Two different uterine cytology diagnoses, culture of uterine flush material and biopsy assay, were performed...
April 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29665557/negative-effects-of-artemisinin-on-phosphorus-solubilizing-bacteria-in-vitro
#8
Huiwen Zeng, Ling Yuan, Jianguo Huang
The anti-malarial drug artemisinin is extracted from the leaves of Artemisia annua L. Due to toxicity to some microorganisms, the release of artemisinin from this medicinal plant in commercial cultivation might produce a potential risk for phosphorus (P) solubilizing bacteria (PSB). Therefore, the growth, P mobilization, and proton and organic acid efflux by two PSB isolates, Bacillus subtilis and Pseudomonas fluorescens, obtained from the soil without growing A. annua L. in history in the region for growing A...
April 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29665368/streptomyces-thermoviolaceus-src3-strain-as-a-novel-source-of-the-antibiotic-adjuvant-streptazolin-a-statistical-approach-toward-the-optimized-production
#9
Ibtissem Djinni, Warda Djoudi, Samiha Souagui, Farida Rabia, Sihem Rahmouni, Ines Mancini, Mouloud Kecha
Streptomyces thermoviolaceus SRC3, a newly isolated actinobacterial strain from Algerian river sediments, exhibited a broad activity against various bacterial and yeast human pathogens (Salmonella typhi ATCC 14028, Vibrio cholerae ATCC 14035, MRSA ATCC 43300 and Candida albicans ATCC 10231). The strain SRC3 was selected from thirty nine actinobacterial isolates and identified as S. thermoviolaceus based on morphology, cultural properties, physiological analyses and 16S rRNA gene sequencing. Culture parameters for the antibiotic production were optimized by sequential statistical strategy including Plackett-Burman design (PBD) and Response Surface Methodology (RSM)...
April 14, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29664370/antimicrobial-resistance-in-invasive-bacterial-infections-in-hospitalized-children-cambodia-2007-2016
#10
Andrew Fox-Lewis, Junko Takata, Thyl Miliya, Yoel Lubell, Sona Soeng, Poda Sar, Kolthida Rith, Gregor McKellar, Vanaporn Wuthiekanun, Erin McGonagle, Nicole Stoesser, Catrin E Moore, Christopher M Parry, Claudia Turner, Nicholas P J Day, Ben S Cooper, Paul Turner
To determine trends, mortality rates, and costs of antimicrobial resistance in invasive bacterial infections in hospitalized children, we analyzed data from Angkor Hospital for Children, Siem Reap, Cambodia, for 2007-2016. A total of 39,050 cultures yielded 1,341 target pathogens. Resistance rates were high; 82% each of Escherichia coli and Klebsiella pneumoniae isolates were multidrug resistant. Hospital-acquired isolates were more often resistant than community-acquired isolates; resistance trends over time were heterogeneous...
May 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29664368/antimicrobial-resistant-bacteria-in-infected-wounds-ghana-2014-1
#11
Hauke Janssen, Iryna Janssen, Paul Cooper, Clemens Kainyah, Theresia Pellio, Michael Quintel, Mathieu Monnheimer, Uwe Groß, Marco H Schulze
Wound infections are an emerging medical problem worldwide, frequently neglected in under-resourced countries. Bacterial culture and antimicrobial drug resistance testing of infected wounds in patients in a rural hospital in Ghana identified no methicillin-resistant Staphylococcus aureus or carbapenem-resistant Enterobacteriaceae but identified high combined resistance of Enterobacteriaceae against third-generation cephalosporins and fluoroquinolones.
May 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29664170/bacterial-screening-of-apheresis-platelets-with-a-rapid-test-a-113-month-single-center-experience
#12
Kristen N Ruby, Reggie R Thomasson, Zbigniew M Szczepiorkowski, Nancy M Dunbar
BACKGROUND: The 2016 Food and Drug Administration draft guidance describes the use of a rapid test (RT) to enhance platelet transfusion safety and availability. This study reports a 113-month experience of screening of apheresis platelets (APs) by RT. STUDY DESIGN AND METHODS: From July 2008 to October 2015, all APs underwent an RT on Day 4. Day 6 and 7 units were transfused with transfusion medicine physician approval. Any units remaining on Day 8 had a second RT performed...
April 17, 2018: Transfusion
https://www.readbyqxmd.com/read/29663934/fructose-enhanced-antimicrobial-activity-of-silver-nanoparticle-embedded-polymersome-nanocarriers
#13
Ilia Gelfat, Benjamin M Geilich, Thomas J Webster
In recent years, an increasing body of research has indicated that the antimicrobial activity of certain antibiotic drugs can be enhanced by the addition of specific metabolites. This study aimed to incorporate these findings into polymersomes (novel polymer-based nanoscale drug delivery vehicles) which can be loaded with various therapeutic molecules and nanoparticles. Polymersome technology has shown promising results in treating antibiotic-resistant infections by co-encapsulating the antibiotic methicillin with silver nanoparticles...
March 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29663668/effect-of-cryopreservation-and-lyophilization-on-viability-and-growth-of-strict-anaerobic-human-gut-microbes
#14
Lea Bircher, Annelies Geirnaert, Frederik Hammes, Christophe Lacroix, Clarissa Schwab
Strict anaerobic gut microbes have been suggested as 'next-generation probiotics' for treating several intestinal disorders. The development of preservation techniques is of major importance for therapeutic application. This study investigated cryopreservation (-80°C) and lyophilization survival and storage stability (4°C for 3 months) of the strict anaerobic gut microbes Bacteroides thetaiotaomicron, Faecalibacterium prausnitzii, Roseburia intestinalis, Anaerostipes caccae, Eubacterium hallii and Blautia obeum...
April 17, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29663550/bacterial-community-structure-and-diversity-in-the-gut-of-muga-silkworm-antheraea-assamensis-lepidoptera-saturniidae-from-india
#15
Sakshi Gandotra, Archna Kumar, Kailash Naga, Pinky Moni Bhuyan, Dip K Gogoi, Kirti Sharma, Sabtharishi Subramanian
Muga silkworm, Antheraea assamensis is exclusively present in the North Eastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture dependent techniques, generic identification using 16s rRNA probes, diversity analysis and qualitative screening for enzyme activities, our studies have identified a number of bacterial isolates viz., Bacillus spp, Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas stutzeri, Acinetobacter sp. and Alcaligens sp...
April 17, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29662492/rnase-h2-loss-in-murine-astrocytes-results-in-cellular-defects-reminiscent-of-nucleic-acid-mediated-autoinflammation
#16
Kareen Bartsch, Markus Damme, Tommy Regen, Lore Becker, Lillian Garrett, Sabine M Hölter, Katharina Knittler, Christopher Borowski, Ari Waisman, Markus Glatzel, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Björn Rabe
Aicardi-Goutières syndrome (AGS) is a rare early onset childhood encephalopathy caused by persistent neuroinflammation of autoimmune origin. AGS is a genetic disorder and >50% of affected individuals bear hypomorphic mutations in ribonuclease H2 (RNase H2). All available RNase H2 mouse models so far fail to mimic the prominent CNS involvement seen in AGS. To establish a mouse model recapitulating the human disease, we deleted RNase H2 specifically in the brain, the most severely affected organ in AGS. Although RNase H2ΔGFAP mice lacked the nuclease in astrocytes and a majority of neurons, no disease signs were apparent in these animals...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29662472/direct-growth-of-bacteria-in-headspace-vials-allows-for-screening-of-volatiles-by-gas-chromatography-mass-spectrometry
#17
Collin M Timm, Evan P Lloyd, Amanda Egan, Ray Mariner, David Karig
Bacterially produced volatile organic compounds (VOCs) can modify growth patterns of eukaryotic hosts and competing/cohabiting microbes. These compounds have been implicated in skin disorders and attraction of biting pests. Current methods to detect and characterize VOCs from microbial cultures can be laborious and low-throughput, making it difficult to understand the behavior of microbial populations. In this work we present an efficient method employing gas chromatography/mass spectrometry with autosampling to characterize VOC profiles from solid-phase bacterial cultures...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29661869/moxifloxacin-replacement-in-contemporary-tuberculosis-drug-regimens-is-ineffective-against-persistent-mycobacterium-tuberculosis-novel-insights-from-the-cornell-mouse-model
#18
Yingjun Liu, Henry Pertinez, Geraint R Davies, Stephen H Gillespie, Anthony R Coates, Yanmin Hu
Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a leading killer worldwide, and disease control is hampered by ineffective control of persistent infections. Substitution of moxifloxacin for isoniazid or ethambutol in standard TB regimens reduces treatment duration and relapse rates in animal studies and four-month regimens were not non-inferior in clinical trials. Resuscitation promoting factor (RPF) dependent bacilli have recently been implicated in M. tuberculosis persistence. We aimed to investigate the therapeutic effects of moxifloxacin substitution in the standard drug regimen for eradicating colony forming count (CFU) positive and RPF-dependent persistent M...
April 16, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29661510/cftr-rescue-with-vx-809-and-vx-770-favors-the-repair-of-primary-airway-epithelial-cell-cultures-from-patients-with-class-ii-mutations-in-the-presence-of-pseudomonas-aeruginosa-exoproducts
#19
Damien Adam, Claudia Bilodeau, Laura Sognigbé, Émilie Maillé, Manon Ruffin, Emmanuelle Brochiero
BACKGROUND: Progressive airway damage due to bacterial infections, especially with Pseudomonas aeruginosa remains the first cause of morbidity and mortality in CF patients. Our previous work revealed a repair delay in CF airway epithelia compared to non-CF. This delay was partially prevented after CFTR correction (with VRT-325) in the absence of infection. Our goals were now to evaluate the effect of the Orkambi combination (CFTR VX-809 corrector + VX-770 potentiator) on the repair of CF primary airway epithelia, in infectious conditions...
April 13, 2018: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29661123/pathological-and-microbiological-study-of-mortality-in-a-captive-breeding-colony-of-the-endangered-lord-howe-island-stick-insect-dryococelus-australis
#20
Christine Bayley, Christina Cheng, Michael Lynch
The authors describe pathological and microbiological features of mortalities in a captive breeding colony of Lord Howe Island stick insects ( Dryococelus australis) over a period of 18 months. There were 2 peaks of mortality in this period. In the first, insects presented dead with minimal premonitory signs of illness. In the second, affected insects were ataxic with contracted limbs and inability to climb or right themselves. Gross lesions were uncommon but included pigmented plaques on the gut and cloacal prolapse...
January 1, 2018: Veterinary Pathology
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