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https://www.readbyqxmd.com/read/28730743/inulin-type-fructans-and-whey-protein-both-modulate-appetite-but-only-fructans-alter-gut-microbiota-in-adults-with-overweight-obesity-a-randomized-controlled-trial
#1
Raylene A Reimer, Holly J Willis, Jasmine M Tunnicliffe, Heekuk Park, Karen L Madsen, Adriana Soto-Vaca
SCOPE: Independently, prebiotics and dietary protein have been shown to improve weight loss and/or alter appetite. Our objective was to determine the effect of combined prebiotic and whey protein on appetite, body composition and gut microbiota in adults with overweight/obesity. METHODS AND RESULTS: In a 12 week, placebo-controlled, double-blind study, 125 adults with overweight/obesity were randomly assigned to receive isocaloric snack bars of: 1) Control; 2) Inulin-type fructans (ITF); 3) Whey protein; 4) ITF + Whey protein...
July 21, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28729389/severe-energy-deficit-up-regulates-leptin-receptors-leptin-signaling-and-ptp1b-in-human-skeletal-muscle
#2
Ismael Perez-Suarez, Jesus Gustavo Ponce-Gonzalez, Jaime de La Calle-Herrero, Jose Losa-Reyna, Marcos Martin-Rincon, David Morales-Alamo, Alfredo Santana, Hans-Christer Holmberg, Jose A L Calbet
In obesity, leptin receptors (OBR) and leptin signaling in skeletal muscle are down-regulated. To determine whether OBR and leptin signaling are up-regulated with a severe energy deficit fifteen overweight men were assessed before (PRE), after 4 days of caloric restriction (3.2 Kcal/kg BW/day) in combination with prolonged exercise (CRE) (8h walking + 45min single-arm cranking/day) to induce an energy deficit of ~5500 Kcal/d, and following 3 days of control diet (isoenergetic) and reduced exercise (CD). During CRE, the diet consisted solely of whey protein (n=8) or sucrose (n=7) (0...
July 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28720963/releasing-characteristics-of-anthocyanins-extract-in-pectin-whey-protein-complex-microcapsules-coated-with-zein
#3
Arranee Chotiko, Subramaniam Sathivel
This study investigated pectin-based capsules as delivery systems for purple rice bran anthocyanin extract (AE) during exposure to simulated gastrointestinal conditions. Four different capsules loaded with AE were prepared by ionotropic gelation/extrusion, including (1) pectin capsules (PE), (2) pectin capsules coated with zein (PE/ZE), (3) pectin-whey protein isolate complex capsules (PE + WP), and (4) pectin-whey protein isolate complex capsules coated with zein (PE + WP/ZE). CaCl2 in an ethanol solution with or without zein was used as a crosslinking solution...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720959/fortification-of-the-whey-protein-isolate-antioxidant-and-antidiabetic-activity-with-fraction-rich-in-phenolic-compounds-obtained-from-stevia-rebaudiana-bert-bertoni-leaves
#4
Paula Gimenez Milani, Maysa Formigoni, Yago Carvalho Lima, Silvano Piovan, Giuliana Maria Ledesma Peixoto, Daiane Montoia Camparsi, Willian do Nascimento da Silva Rodrigues, Jordana Quaglia Pereira da Silva, Alexandre da Silva Avincola, Eduardo Jorge Pilau, Cecília Edna Mareze da Costa, Silvio Cláudio da Costa
A stevia fraction (ASF) free of steviol glycosides was extracted from Stevia rebaudiana leaves (Stevia UEM-13). ASF essentially constitutes phenolic compounds (52.42%), which were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) as caffeic acid, quercetin-3-o-glycoside, cyanidin-3-glucoside, kaempferol, quercetin, apigenin, rozmarinic acid, chlorogenic acid and dicaffeoylquinic acid. ASF was used as a multi-functional source of phenolic compounds to fortify the whey protein isolate (WPI) obtained by membrane separation...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720947/reduction-of-whey-protein-concentrate-antigenicity-by-using-a-combined-enzymatic-digestion-and-ultrafiltration-approach
#5
Laura Quintieri, Linda Monaci, Federico Baruzzi, Maria Gabriella Giuffrida, Silvia de Candia, Leonardo Caputo
The global interest in saving food resources is leading to recycle wasted-food materials to extract useful nutrients. In dairy industry, the recycling of whey proteins determines their utilization in the healthy-addressed foods, which, however, can cause immunological responses in allergic subjects. In this work, a whey protein concentrate (WPC) was alternatively hydrolyzed with pepsin, papain, trypsin and rennin in order to attenuate or abolish the β-lactoglobulin (BLG) antigenicity. The electrophoretic profiles of both pepsin and papain WPC hydrolysates proved the disappearance of the BLG band, even though a slight antigenicity was still found by ELISA...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28719669/a-whey-protein-based-multi-ingredient-nutritional-supplement-stimulates-gains-in-lean-body-mass-and-strength-in-healthy-older-men-a-randomized-controlled-trial
#6
Kirsten E Bell, Tim Snijders, Michael Zulyniak, Dinesh Kumbhare, Gianni Parise, Adrian Chabowski, Stuart M Phillips
Protein and other compounds can exert anabolic effects on skeletal muscle, particularly in conjunction with exercise. The objective of this study was to evaluate the efficacy of twice daily consumption of a protein-based, multi-ingredient nutritional supplement to increase strength and lean mass independent of, and in combination with, exercise in healthy older men. Forty-nine healthy older men (age: 73 ± 1 years [mean ± SEM]; BMI: 28.5 ± 1.5 kg/m2) were randomly allocated to 20 weeks of twice daily consumption of either a nutritional supplement (SUPP; n = 25; 30 g whey protein, 2...
2017: PloS One
https://www.readbyqxmd.com/read/28712368/inferring-genetic-parameters-on-latent-variables-underlying-milk-yield-and-quality-protein-composition-curd-firmness-and-cheese-making-traits-in-dairy-cattle
#7
C Dadousis, C Cipolat-Gotet, G Bittante, A Cecchinato
We studied the genetics of cheese-related latent variables (factors; Fs) for application in dairy cattle breeding. In total, 26 traits, recorded in 1264 Brown Swiss cows, were analyzed through multivariate factor analysis (MFA). Traits analyzed were descriptors of milk quality and yield (including protein fractions) and measures of coagulation, curd firmness (CF), cheese yields (%CY) and nutrient recoveries in the curd (REC). A total of 10 Fs (mutual orthogonal with a varimax rotation) were obtained. To assess the practical use of the Fs into breeding, we inferred their genetic parameters using single and bivariate animal models under a Bayesian framework...
July 17, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28711271/identification-of-dipeptidyl-peptidase-iv-inhibitory-peptides-from-mare-whey-protein-hydrolysates
#8
J J Song, Q Wang, M Du, X M Ji, X Y Mao
Inhibition of dipeptidyl peptidase-IV (DPP-IV) activity is a promising strategy for treatment of type 2 diabetes. In current study, DPP-IV inhibitory peptides were identified from mare whey protein hydrolysates obtained by papain. The results showed that all the mare whey protein hydrolysates obtained at various hydrolysis durations possessed more potent DPP-IV inhibitory activity in comparison with intact whey protein. The 4-h hydrolysates showed the greatest DPP-IV inhibitory activity with half-maximal inhibitory concentration of 0...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28709970/selective-effects-of-whey-protein-concentrate-on-glutathione-levels-and-apoptosis-in-rats-with-mammary-tumors
#9
Shih-Hsuan Cheng, Yang-Ming Tseng, Szu-Hsien Wu, Shih-Meng Tsai, Li-Yu Tsai
Glutathione (GSH) plays an important role in antioxidant defense and regulation of apoptosis. GSH deficiency is related to many diseases, including cancer, and increased GSH levels in cancer cells are associated with chemotherapy resistance because of resistance to apoptosis. In this study, we investigated the effects of whey protein concentrate (WPC), a precursor of GSH, in rats with mammary tumors induced by treatment with 7,12-dimethylbenz(a)anthracene (DMBA). DMBA treatment results in cellular changes that mimic the initiation and promotion of carcinogenesis of breast tissue...
July 11, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28702650/influence-of-calcium-induced-droplet-heteroaggregation-on-the-physicochemical-properties-of-oppositely-charged-lactoferrin-coated-lutein-droplets-and-whey-protein-isolate-coated-dha-droplets
#10
Xin Li, Xu Wang, Duoxia Xu, Yanping Cao, Shaojia Wang, Bei Wang, Chengtao Wang, Baoguo Sun
The influence of calcium-induced droplet heteroaggregation on the formation and physicochemical stability of mixed lutein and DHA emulsions was studied. Heteroaggregation was induced by mixing oppositely charged lactoferrin (LF)-coated lutein and whey protein isolate (WPI)-coated DHA emulsions with different CaCl2 concentrations at pH 6.0. The droplet size, zeta-potential, transmission-physical stability and microstructure behavior (CLSM and Cryo-SEM) of single-protein emulsions and mixed emulsions were measured as a function of different CaCl2 concentrations...
July 12, 2017: Food & Function
https://www.readbyqxmd.com/read/28698349/a-pilot-randomised-controlled-trial-of-a-periodised-resistance-training-and-protein-supplementation-intervention-in-prostate-cancer-survivors-on-androgen-deprivation-therapy
#11
Jacqueline L Kiwata, Tanya B Dorff, E Todd Schroeder, George J Salem, Christianne J Lane, Judd C Rice, Mitchell E Gross, Christina M Dieli-Conwright
INTRODUCTION: Prostate cancer survivors (PCS) receiving androgen deprivation therapy (ADT) experience deleterious side effects such as unfavourable changes in cardiometabolic factors that lead to sarcopenic obesity and metabolic syndrome (MetS). While loss of lean body mass (LBM) compromises muscular strength and quality of life, MetS increases the risk of cardiovascular disease and may influence cancer recurrence. Exercise can improve LBM and strength, and may serve as an alternative to the pharmacological management of MetS in PCS on ADT...
July 10, 2017: BMJ Open
https://www.readbyqxmd.com/read/28696380/whey-protein-supplementation-enhances-whole-body-protein-metabolism-and-performance-recovery-after-resistance-exercise-a-double-blind-crossover-study
#12
Daniel W D West, Sidney Abou Sawan, Michael Mazzulla, Eric Williamson, Daniel R Moore
No study has concurrently measured changes in free-living whole body protein metabolism and exercise performance during recovery from an acute bout of resistance exercise. We aimed to determine if whey protein ingestion enhances whole body net protein balance and recovery of exercise performance during overnight (10 h) and 24 h recovery after whole body resistance exercise in trained men. In a double-blind crossover design, 12 trained men (76 ± 8 kg, 24 ± 4 years old, 14% ± 5% body fat; means ± standard deviation (SD)) performed resistance exercise in the evening prior to consuming either 25 g of whey protein (PRO; MuscleTech 100% Whey) or an energy-matched placebo (CHO) immediately post-exercise (0 h), and again the following morning (~10 h of recovery)...
July 11, 2017: Nutrients
https://www.readbyqxmd.com/read/28693995/application-of-whey-protein-pectin-nano-complex-carriers-for-loading-of-lactoferrin
#13
Masoomeh Raei, Fakhri Shahidi, Majid Farhoodi, Seid Mahdi Jafari, Ali Rafe
Our aim was to entrap lactoferrin (LF) in complex nano-particles of whey protein isolate (WPI)-high methoxyl pectin (HMP) with the ratios of 2:1, 1:1, and 1:2 through acidification at pH values of 3, 3.5, and 4. The zeta-potential, size, sedimentable-complex yield, LF content, encapsulation efficiency, SEM, AFM, FTIR, and DSC of nano-particles were investigated. Our results revealed that almost all analyzed parameters of the final nano-particles were related to preparation pH value, WPI/HMP ratios, and acidification methods In both methods of pre- and post-acidification, the zeta potential was decreased via decreasing of pH from 4 to 3 and particle size was increased at higher HMP ratios to WPI...
July 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28688363/modulating-in-vitro-gastric-digestion-of-emulsions-using-composite-whey-protein-cellulose-nanocrystal-interfaces
#14
Anwesha Sarkar, Shuning Zhang, Brent Murray, Jessica A Russell, Sally Boxal
In this study, we designed emulsions with an oil-water interface consisting of a composite layer of whey protein isolate (WPI, 1wt%) and cellulose nanocrystals (CNCs) (1-3wt%). The hypothesis was that a secondary layer of CNCs at the WPI-stabilized oil-water interface could protect the interfacial protein layer against in vitro gastric digestion by pepsin at 37°C. A combination of transmission electron microscopy, ζ-potential measurements, interfacial shear viscosity measurements and theoretical surface coverage considerations suggested the presence of CNCs and WPI together at the O/W interface, owing to the electrostatic attraction between complementarily charged WPI and CNCs at pH 3...
June 23, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28686292/microencapsulation-of-borage-oil-with-blends-of-milk-protein-%C3%AE-glucan-and-maltodextrin-through-spray-drying-physicochemical-characteristics-and-stability-of-the-microcapsules
#15
Ru-Yi Li, Yan Shi
BACKGROUND: Borage oil is a rich commercial source of gamma-linolenic acid (GLA, 18:3n-6). However, borage oil is rich in omega-6 polyunsaturated fatty acids and vulnerable to oxidation. Thus, selecting of appropriate wall materials is critical to the encapsulation of borage oil. This study investigated the influence of wall materials on the physicochemical characteristics and stability of microencapsulated borage oil by spray drying. Blends of milk protein [sodium caseinate (CAS) or whey protein concentrate (WPC)], β-glucan (GLU) and maltodextrin (MD) were used as the wall materials for encapsulating borage oil...
July 7, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28681702/wound-healing-time-to-look-for-intelligent-natural-immunological-approaches
#16
Olivier Garraud, Wael N Hozzein, Gamal Badr
There is now good evidence that cytokines and growth factors are key factors in tissue repair and often exert anti-infective activities. However, engineering such factors for global use, even in the most remote places, is not realistic. Instead, we propose to examine how such factors work and to evaluate the reparative tools generously provided by 'nature.' We used two approaches to address these objectives. The first approach was to reappraise the internal capacity of the factors contributing the most to healing in the body, i...
June 21, 2017: BMC Immunology
https://www.readbyqxmd.com/read/28679035/partially-hydrolyzed-whey-proteins-prevent-clinical-symptoms-in-a-cow-s-milk-allergy-mouse-model-and-enhance-regulatory-t-and-b-cell-frequencies
#17
M B Gea Kiewiet, B C A M van Esch, J Garssen, M M Faas, P de Vos
SCOPE: Partially hydrolyzed cow's milk proteins are used to prevent cow's milk allergy in children. Here we studied the immunomodulatory mechanisms of partial cow's milk hydrolysates in vivo. METHODS AND RESULTS: Mice were sensitized with whey or partially hydrolyzed whey using cholera toxin. Whey-specific IgE levels were measured to determine sensitization and immune cell populations from spleen, mesenteric lymph nodes and Peyer's patches after oral whey administration were measured by flowcytometry...
July 5, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28668535/insights-into-the-mechanism-of-the-formation-of-the-most-stable-crystal-polymorph-of-milk-fat-in-model-protein-matrices
#18
P R Ramel, A G Marangoni
The effect of incorporation and presence of various ingredients in a model sodium caseinate-based imitation cheese matrix on the polymorphism of milk fat was comprehensively described using powder x-ray diffraction, differential scanning calorimetry, and microscopy. With anhydrous milk fat (AMF) in bulk used as control, the embedding of AMF as droplets in a protein matrix was found to result in a greater extent of formation of the β polymorph than AMF alone and AMF homogenized with water and salts solution...
June 28, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28667710/determination-of-nitrite-in-milk-and-soy-based-nutritional-ingredients-by-derivatization-with-2-3-diaminonaphthalene-and-fluorescence-spectrometry
#19
Stefan Ehling, Todime M Reddy
Nitrite (NO₂(–)) is an inorganic anion that can be foundin various powdered milk- and soy-based nutritional ingredients as an incidental contaminant. Reliable determination of NO₂(–) in nutritional ingredients is of paramount importance to ensure the safety of finished products. The derivatization reaction of NO₂(–) with 2,3-diaminonaphthalene with the formation of fluorescent 2,3-naphtotriazole has been adapted to milk- and soy-based nutritional ingredients. The sample preparation consisted of protein precipitation with Carrez solution, simple pass-through cleanup of extracts utilizing a carbon black-based cartridge and derivatization, followed by batch fluorometry...
June 30, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28666158/interaction-of-sucralose-with-whey-protein-experimental-and-molecular-modeling-studies
#20
Hongmei Zhang, Shixin Sun, Yanqing Wang, Jian Cao
The objective of this research was to study the interactions of sucralose with whey protein isolate (WPI) by using the three-dimensional fluorescence spectroscopy, circular dichroism spectroscopy and molecular modeling. The results showed that the peptide strands structure of WPI had been changed by sucralose. Sucralose binding induced the secondary structural changes and increased content of aperiodic structure of WPI. Sucralose decreased the thermal stability of WPI and acted as a structure destabilizer during the thermal unfolding process of protein...
June 27, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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