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MiRNA 27a

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https://www.readbyqxmd.com/read/29143999/microrna-27a-3p-inhibits-cell-viability-and-migration-through-down-regulating-dusp16-in-hepatocellular-carcinoma
#1
Jin-Mao Li, Jun Zhou, Zhen Xu, Hai-Jin Huang, Min-Jiang Chen, Jian-Song Ji
MicroRNA (miRNA or miR) has been shown to play an important role in the initiation and development in many different cancers. Here, we demonstrated down-regulated expression of miR-27a-3p in hepatocellular carcinoma (HCC) tissues in comparison with that in adjacent normal liver tissues based on the TCGA database. Cells viability and apoptosis was measured by CCK-8 and flow cytometry assay. Cell invasion and migration was measured by Transwell and wound healing assay. The effect of miR-27a-3p on DUSP16 expression was evaluated by luciferase assays, and western blot assay...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29142030/plasma-microrna-profiles-in-patients-with-early-rheumatoid-arthritis-responding-to-adalimumab-plus-methotrexate-vs-methotrexate-alone-a-placebo-controlled-clinical-trial
#2
Jacob Sode, Sophine B Krintel, Anting Liu Carlsen, Merete L Hetland, Julia S Johansen, Kim Hørslev-Petersen, Kristian Stengaard-Pedersen, Torkell Ellingsen, Mark Burton, Peter Junker, Mikkel Østergaard, Niels H H Heegaard
OBJECTIVE: The aim was to identify plasma (i.e., cell-free) microRNA (miRNA) predicting antitumor necrosis and/or methotrexate (MTX) treatment response in patients enrolled in an investigator-initiated, prospective, double-blinded, placebo-controlled trial (The OPERA study, NCT00660647). METHODS: We included 180 disease-modifying antirheumatic drug-naive patients with early rheumatoid arthritis (RA) randomized to adalimumab (ADA; n = 89) or placebo (n = 91) in combination with MTX...
November 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/29128203/study-of-correlation-between-genetic-variants-in-three-microrna-genes-hsa-mir-146a-hsa-mir-502-binding-site-hsa-mir-27a-and-breast-cancer-risk
#3
S Parchami Barjui, S Reiisi, S O Ebrahimi, B Shekari
BACKGROUND AND AIM: MicroRNAs (miRNAs) represent noncoding RNAs that play a role in the gene regulation of oncogenes and tumor suppressors. Single nucleotide polymorphisms (SNPs) in miRNA genes are associated with risk of cancer. Some of these SNPs are more common including rs2910164 in the miR-146a, rs895819 in the miR-27a, and rs2910164 in the miR-502 binding site of the SET8. Our aim was to investigate the association between these polymorphisms and the risk of breast cancer in an Iranian population...
November 8, 2017: Current Research in Translational Medicine
https://www.readbyqxmd.com/read/29115608/microrna%C3%A2-27a-promotes-tumorigenesis-via-targeting-akt-in-triple-negative-breast-cancer
#4
Jing Wu, Zhihui Sun, Huijie Sun, Yanhua Li
Altered microRNA (miRNA/miR) expression regulates tumor development and progression in triple‑negative breast cancer (TNBC). The present study examined the effect of miR‑27a on proliferation, migration and invasion of TNBC cells in vitro and in vivo. An MTT assay was performed to examine the proliferation of MDA‑MB‑231 and MDA‑MB‑468 breast cancer cells with either overexpression of miR‑27a or downregulation of miR‑27a, in the presence or absence of radiation. The migratory and invasive abilities of MDA‑MB‑231 and MDA‑MB‑468 breast cancer cells were assessed by Transwell migration and Matrigel invasion assays...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29100439/genetic-polymorphisms-in-pre-mirnas-predict-the-survival-of-non-small-cell-lung-cancer-in-chinese-population-a-cohort-study-and-a-meta-analysis
#5
Lingzi Xia, Zhihua Yin, Xuelian Li, Yangwu Ren, Haibo Zhang, Yuxia Zhao, Baosen Zhou
Background: To explore the association of genetic polymorphisms in pre-miRNA 30c-1 rs928508 and pre-miRNA 27a rs895819 with non-small-cell lung cancer prognosis. Materials and Methods: 480 patients from five hospitals were enrolled in this prospective cohort study. They were followed up for five years. The association between genotypes and overall survival was assessed by Cox proportional hazards regression models. A meta-analysis was conducted to provide evidence for the effect of microRNA 27a rs895819 on cancer survival...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100393/down-regulation-of-traditional-oncomirs-in-plasma-of-breast-cancer-patients
#6
Dana Jurkovicova, Bozena Smolkova, Monika Magyerkova, Zuzana Sestakova, Viera Horvathova Kajabova, Ludovit Kulcsar, Iveta Zmetakova, Lenka Kalinkova, Tomas Krivulcik, Marian Karaba, Juraj Benca, Tatiana Sedlackova, Gabriel Minarik, Zuzana Cierna, Ludovit Danihel, Michal Mego, Miroslav Chovanec, Ivana Fridrichova
Deregulated expression of microRNAs has the oncogenic or tumor suppressor function in cancer. Since miRNAs in plasma are highly stable, their quantification could contribute to more precise cancer diagnosis, prognosis and therapy prediction. We have quantified expression of seven oncomiRs, namely miR-17/92 cluster (miR-17, miR-18a, miR-19a and miR-20a), miR-21, miR-27a and miR-155, in plasma of 137 breast cancer (BC) patients. We detected down-regulation of six miRNAs in patients with invasive BC compared to controls; however, only miR-20a and miR-27a down-regulations were statistically significant...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100284/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#7
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29098032/identification-of-mirna-and-genes-involving-in-osteosarcoma-by-comprehensive-analysis-of-microrna-and-copy-number-variation-data
#8
Tao Luo, Xiangli Yi, Wei Si
The aim of the present study was to understand the molecular mechanisms of osteosarcoma by comprehensive analysis of microRNA (miRNA/miR) and copy number variation (CNV) microarray data. Microarray data (GSE65071 and GSE33153) were downloaded from the Gene Expression Omnibus. In GSE65071, differentially expressed miRNAs between the osteosarcoma and control groups were calculated by the Limma package. Target genes of differentially expressed miRNAs were identified by the starBase database. For GSE33153, PennCNV software was used to perform the copy number variation (CNV) analysis...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29096313/regulation-of-pregnane-x-receptor-and-micrornas-on-detoxification-related-genes-expressions-in-mugilogobius-abei-under-the-exposure-to-diclofenac
#9
Peijia Ku, Chao Wang, Xiangping Nie, Ruikang Ou, Kaibing Li
Diclofenac (DCF) has been recognized as an emerging contaminant in aquatic environments. Though many studies have investigated the toxic effects of DCF in human and mammals, limited information is available for the responses of genes associated with detoxification metabolisms in non-target aquatic organisms such as fish. In the present study, a small benthic fish Mugilogobius abei, was chosen as the test organism and the effects of DCF on detoxification-related genes at transcriptional level in M. abei were investigated...
October 30, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29089199/microrna-27a-3p-affects-estradiol-and-androgen-imbalance-by-targeting-creb1-in-the-granulosa-cells-in-mouse-polycytic-ovary-syndrome-model
#10
Mingming Wang, Mei Liu, Jing Sun, Lina Jia, Shuoqian Ma, Jun Gao, Yunyun Xu, Hongwen Zhang, Suk Ying Tsang, Xiangdong Li
Polycystic ovary syndrome (PCOS) is a common endocrine abnormality in women characterized by a menstrual disturbance with chronic anovulation and hyperandrogenism, polycystic ovaries, and insulin resistance. MicroRNAs (miRNAs) are important fine-tune regulators involved in various physiological and pathological processes, but their actions are not fully understood. In this study, we observed the increased expression of miR-27a-3p in the ovaries of mice with PCOS and explored its functions in primary mouse granulosa cells (mGCs) and the mouse granulosa-like tumor cell line, KK-1, using several approaches...
October 28, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29065177/mir-27a-induced-by-colon-cancer-cells-in-hlecs-promotes-lymphangiogenesis-by-targeting-smad4
#11
Qi Xu, Jin-Lu Tong, Chen-Peng Zhang, Qian Xiao, Xiao-Lin Lin, Xiu-Ying Xiao
AIM: Metastasis of tumor cells occurs through lymphatic vessels, blood vessels and transcoelomic spreading. Growing evidence from in vivo and in vitro studies has indicated that tumor lymphangiogenesis facilitates metastasis. However, the regulation of lymphangiogenesis in colon cancer remains unclear. The aims of this study were to identify key miRNAs in colon cancer lymphangiogenesis and to investigate its target and mechanism. METHODS: miRNA microarray analysis was conducted to identify miRNAs in human lymphatic endothelial cells (HLECs) that were regulated by co-cultured human colon cancer cells...
2017: PloS One
https://www.readbyqxmd.com/read/28990745/diagnostic-value-of-circulating-microrna-27a-b-in-patients-with-acute-pulmonary-embolism
#12
Qian Wang, Junfen Ma, Zhiyun Jiang, Fan Wu, Jiedan Ping, Liang Ming
BACKGROUND: Circulating microRNAs (miRNAs) have been increasingly suggested as biomarkers for numerous diseases. The aims of this study were to evaluate the expression of plasma miR-27a/b in patients with acute pulmonary embolism (APE) and determine the possibility of miR-27a/b as diagnostic biomarkers for APE. METHODS: 78 APE patients diagnosed by computed tomographic pulmonary angiography (CTPA) and 70 age and gender matched normal volunteers were included in this study...
October 9, 2017: International Angiology: a Journal of the International Union of Angiology
https://www.readbyqxmd.com/read/28983252/evaluation-of-muscle-microrna-expression-in-relation-to-human-peripheral-insulin-sensitivity-a-cross-sectional-study-in-metabolically-distinct-subject-groups
#13
Dennis Dahlmans, Alexandre Houzelle, Johanna A Jörgensen, Esther Phielix, Lucas Lindeboom, Matthijs K C Hesselink, Patrick Schrauwen, Joris Hoeks
In recent years, several microRNAs (miRNAs)-post-transcriptional regulators of gene expression-have been linked to the regulation of peripheral insulin sensitivity. Many of these studies, however, have been conducted in cell or animal models and the few human studies available lack adequate measurements of peripheral insulin sensitivity. In the present study, we examined the expression of 25 miRNAs, putatively involved in (peripheral) insulin sensitivity, in skeletal muscle biopsies from extensively phenotyped human individuals, widely ranging in insulin sensitivity...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28974022/effect-of-green-and-brown-propolis-extracts-on-the-expression-levels-of-micrornas-mrnas-and-proteins-related-to-oxidative-stress-and-inflammation
#14
Vincenzo Zaccaria, Valeria Curti, Arianna Di Lorenzo, Alessandra Baldi, Cristina Maccario, Sabrina Sommatis, Roberto Mocchi, Maria Daglia
A large body of evidence highlights that propolis exerts many biological functions that can be ascribed to its antioxidant and anti-inflammatory components, including different polyphenol classes. Nevertheless, the molecular mechanisms are yet unknown. The aim of this study is to investigate the mechanisms at the basis of propolis anti-inflammatory and antioxidant activities. The effects of two brown and green propolis extracts-chemically characterized by RP-HPLC-PDA-ESI-MSn-on the expression levels of miRNAs associated with inflammatory responses (miR-19a-3p and miR-203a-3p) and oxidative stress (miR-27a-3p and miR-17-3p), were determined in human keratinocyte HaCat cell lines, treated with non-cytotoxic concentrations...
October 1, 2017: Nutrients
https://www.readbyqxmd.com/read/28964816/quinalizarin-inhibits-adipogenesis-through-down-regulation-of-transcription-factors-and-microrna-modulation
#15
Lisa Schwind, Lisa Nalbach, Andreas D Zimmer, Katja B Kostelnik, Jennifer Menegatti, Friedrich Grässer, Claudia Götz, Mathias Montenarh
BACKGROUND: Protein kinase CK2 is induced early in adipogenesis whereas later on, this kinase seems to be dispensable. Here, we have analysed how CK2 might be involved in early steps of differentiation of 3T3-L1 cells. METHODS: 3T3-L1 cells were differentiated to adipocytes in the absence or presence of quinalizarin. The expression and localization of important transcription factors was analysed by Western blot and immunofluorescence. DNA binding capacity and transactivation was analysed with pull-down assays and with luciferase reporter experiments, respectively...
December 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28953910/potential-mirna-involvement-in-the-anti-adipogenic-effect-of-resveratrol-and-its-metabolites
#16
Itziar Eseberri, Arrate Lasa, Jonatan Miranda, Ana Gracia, Maria P Portillo
OBJECTIVE: Scientific research is constantly striving to find molecules which are effective against excessive body fat and its associated complications. Taking into account the beneficial effects that resveratrol exerts on other pathologies through miRNA, the aim of the present work was to analyze the possible involvement of miRNAs in the regulation of adipogenic transcription factors peroxisome proliferator-activated receptor γ (pparγ), CCAAT enhancer-binding proteins α and β (cebpβ and cebpα) induced by resveratrol and its metabolites...
2017: PloS One
https://www.readbyqxmd.com/read/28922434/association-of-microrna-polymorphisms-with-the-risk-of-gastric-cancer-in-a-romanian-population
#17
Ion Rogoveanu, Florin Burada, Mihai Gabriel Cucu, Cristin Constantin Vere, Mihai Ioana, Raluca Alexandra Cîmpeanu
BACKGROUND AND AIMS: MicroRNAs (miRNAs) play an important role in the occurrence and progression of human cancers, including gastric cancer. Our hospital-based case-control study aimed to investigate whether four commonly studied single nucleotide polymorphisms (SNPs) have effects on susceptibility to gastric cancer in a Romanian population. METHOD: We genotyped the miR-27a rs895819, miR-146a rs2910164, miR-196a2 rs11614913 and miR-499 rs3746444 SNPs by real-time PCR using predesignated TaqMan assays in 430 individuals (142 gastric cancer patients and 288 age and gender matched cancer-free controls)...
September 2017: Journal of Gastrointestinal and Liver Diseases: JGLD
https://www.readbyqxmd.com/read/28920467/annals-express-association-of-subcutaneous-and-visceral-fat-with-circulating-micrornas-in-a-middle-aged-japanese-population
#18
Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki, Yoshiki Tsuboi, Mari Kondo, Genki Mizuno, Hiroaki Ishikawa, Keiko Sugimoto, Keisuke Osakabe, Naohiro Ichino, Koji Ohashi, Nobuyuki Hamajima, Koji Suzuki
Purpose It has been demonstrated that circulating microRNA (miRNA) profiles are affected by physiological conditions. Several studies have demonstrated that miRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating miRNAs and obesity, which has become a major public health problem worldwide. Current study investigated the association between circulating miRNAs and obesity in a Japanese population. Methods Obesity parameters, such as subcutaneous (SAT) and visceral fat (VAT) adipose tissue, body fat percentage, and body mass index (BMI) were assessed in a cross-sectional sample of 526 participants who attended health examinations in Yakumo, Japan...
January 1, 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28916633/systems-genetics-identifies-a-co-regulated-module-of-liver-micrornas-associated-with-plasma-ldl-cholesterol-in-murine-diet-induced-dyslipidemia
#19
Alisha R Coffey, Tangi L Smallwood, Jody Albright, Kunjie Hua, Matt Kanke, Daniel Pomp, Brian J Bennett, Praveen Sethupathy
Chronically altered levels of circulating lipids, termed dyslipidemia, is a significant risk factor for a number of metabolic and cardiovascular morbidities. MicroRNAs (miRNAs) have emerged as important regulators of lipid balance, have been implicated in dyslipidemia, and have been proposed as candidate therapeutic targets in lipid-related disorders including atherosclerosis. A major limitation of most murine studies of miRNAs in lipid metabolic disorders is that they have been performed in just one (or very few) inbred strains, such as C57BL/6...
November 1, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28892167/effect-of-cadmium-on-kitl-pre-mrna-alternative-splicing-in-murine-ovarian-granulosa-cells-and-its-associated-regulation-by-mirnas
#20
Wenxiang Wang, Jie Chen, Lingfeng Luo, Yuchen Li, Jin Liu, Wenchang Zhang
In this study, we established an in vitro exposure model of murine ovarian granulosa cells to observe the effect of Cd on alternative splicing of the kitl pre-mRNA and subsequently to explore the role of kitl gene expression regulation-related miRNAs through miRNA prediction, miRNA chip, bioinformatics and real-time quantitative polymerase chain reaction analyses. Our results showed that the kitl1/kitl2 mRNA ratio was significantly different (P < 0.05) at different dosages and times. The miRNA chip analysis showed that the miRNA expression profiles for the Cd treatment were significantly changed, and the expression of 29 miRNAs involved in alternative splicing of the kitl pre-mRNA was changed...
September 11, 2017: Journal of Applied Toxicology: JAT
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