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https://www.readbyqxmd.com/read/29453975/mir144-3p-inhibits-pmvecs-excessive-proliferation-in-angiogenesis-of-hepatopulmonary-syndrome-via-tie2
#1
Congwen Yang, Keyi Lv, Bin Chen, Yong Yang, Xiangfa Ai, Hongfu Yu, Yihui Yang, Bin Yi, Kaizhi Lu
BACKGROUND/AIM: Increasing evidence show microRNAs (miRNAs) are associated with hepatopulmonary syndrome (HPS). The aim of this study was to investigate the role of miR-144 in the angiogenesis of HPS, as well as to identify its underlying mechanism. METHODS: The expression levels of miR-144-3p were assessed in pulmonary micro-vascular endothelial cells (PMVECs), as well as in lung tissues from rats with HPS. We predicted the potential target of miR-144-3p. Tyrosine kinase 2(Tie2) was identified as a target gene of miR144-3p, which has an essential role in the angiogenesis of lung vessel...
February 14, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29448950/identification-and-characterization-of-microrna-in-the-lung-tissue-of-pigs-with-different-susceptibilities-to-pcv2-infection
#2
Ping Zhang, Liyuan Wang, Yanping Li, Ping Jiang, Yanchao Wang, Pengfei Wang, Li Kang, Yuding Wang, Yi Sun, Yunliang Jiang
Porcine circovirus type 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases. According to our previous RNA-sequencing analysis, the differences in the susceptibility to PCV2 infection depended on the genetic differences between the Laiwu (LW) and Yorkshire × Landrace crossbred (YL) pigs, but the cellular microRNA (miRNA) that are differentially expressed between the LW and YL pigs before and after PCV2 infection remain to be determined. In this study, high-throughput sequencing was performed to determine the abundance and differential expression of miRNA in lung tissues from PCV2-infected and PCV2-uninfected LW and YL pigs...
February 15, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29445170/brm270-inhibits-cancer-stem-cell-maintenance-via-microrna-regulation-in-chemoresistant-a549-lung-adenocarcinoma-cells
#3
Taeho Kwon, Nisansala Chandimali, Do Luong Huynh, Jiao Jiao Zhang, Nameun Kim, Yesol Bak, Do-Young Yoon, Dae-Yeul Yu, Jae Cheol Lee, Meeta Gera, Mrinmoy Ghosh, Yang Ho Park, Dong Kee Jeong
Chemotherapy is a standard treatment for non-small-cell lung cancer (NSCLC). However, the dose-limiting toxicity of drugs and the development of chemoresistance are major clinical challenges to successful management of NSCLC. Asian traditional medicine is gaining global attention as a non-toxic alternative to chemotherapy. BRM270 is an extract formulated from seven Asian medicinal plants that has been shown to inhibit tumor cell proliferation in diverse cancer types. We previously demonstrated that BRM270 suppresses tumorigenesis by negatively regulating nuclear factor-κB signaling in multidrug-resistant cancer stem cells (CSCs)...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29445125/pin1-impairs-microrna-biogenesis-by-mediating-conformation-change-of-xpo5-in-hepatocellular-carcinoma
#4
Jiao Li, Wenchen Pu, Hui-Lung Sun, Jian-Kang Zhou, Xin Fan, Yuanyuan Zheng, Juan He, Xuesha Liu, Zhichu Xia, Lunxu Liu, Yu-Quan Wei, Yong Peng
MicroRNA (miRNA) dysregulation is associated with the tumorigenesis and development of numerous human cancers. The defect in miRNA biogenesis is the main cause of miRNA dysregulation. We previously demonstrated that ERK-induced phosphorylation of XPO5 followed by peptidyl-prolyl cis/trans isomerase Pin1-mediated isomerization downregulates miRNA expression and contributes to hepatocellular carcinoma (HCC) development. However, how Pin1 precisely regulates miRNA biogenesis in HCC remains elusive. Here we reveal that Pin1 has a pivotal role in the miRNA maturation process by modulating phosphorylated Serine-Proline (pS-P) motif of XPO5 in a phosphorylation-dependent manner...
February 14, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29440731/microarray-profile-of-circular-rnas-identifies-hsa_circ_0014130-as-a-new-circular-rna-biomarker-in-non-small-cell-lung-cancer
#5
Shaoyan Zhang, Xiaoli Zeng, Ting Ding, Lin Guo, Yulong Li, Songlei Ou, Hui Yuan
Accumulating evidence has revealed that aberrant Circular RNAs (circRNAs) expression plays important roles in carcinogenesis and tumor progression. However, their role in non-small cell lung cancer (NSCLC) remains unclear. In this study, we first used circRNA microarrays to screen for tumour-specific circRNA candidates in between NSCLC (n = 3) and adjacent lung (n = 3) tissue. Among the circRNA expression profile, two circRNAs (hsa_circ_0014130 and hsa_circ_0016760) were selected for validation in ten pairs of NSCLC and adjacent non-cancerous tissues by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR)...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440630/human-umbilical-cord-mesenchymal-stem-cell-derived-extracellular-vesicles-promote-lung-adenocarcinoma-growth-by-transferring-mir-410
#6
Liyang Dong, Yanan Pu, Lina Zhang, Qianqian Qi, Lei Xu, Wei Li, Chuan Wei, Xiaofan Wang, Sha Zhou, Jifeng Zhu, Xuefeng Wang, Feng Liu, Xiaojun Chen, Chuan Su
Although accumulating evidence has linked mesenchymal stem cells (MSCs) with tumor growth, the underlying mechanisms are poorly understood. Here, we demonstrated for the first time that human umbilical cord MSCs (hUCMSCs) dramatically increased the growth of lung adenocarcinoma (LUAD) cancer cells in a xenograft tumor model. Then, we observed that hUCMSC-derived extracellular vesicles (hUCMSC-EVs) contribute to the hUCMSC-promoted LUAD cell growth through a direct effect on LUAD cells. Furthermore, we showed that hUCMSC-EV-mediated LUAD growth is associated with increased proliferation and decreased apoptosis in LUAD cells, concomitant with reduced PTEN expression mediated by the hUCMSC-EV-transmitted miR-410...
February 13, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29435942/identification-and-isolation-of-novel-sugar-like-rna-protecting-materials-glycylglycerins-from-pluripotent-stem-cells
#7
Shi-Lung Lin
Pluripotent stem cells are a resourceful treasure box for regenerative medicine. They contain a large variety of novel materials useful for designing and developing new medicines and therapies directed against many aging-associated degenerative disorders, including Alzheimer's disease, Parkinson's disease, stroke, diabetes, osteoporosis, and cancers. Currently, identification of these novel stem cell-specific materials is one of major breakthroughs in the field of stem cell research. Particularly, since the discovery of induced pluripotent stem cells (iPSC) in year 2006, the methods of iPSC derivation further provide an unlimited resource for screening, isolating, and even producing theses novel stem cell-specific materials in vitro...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435941/the-mir-302-mediated-induction-of-pluripotent-stem-cells-ipsc-multiple-synergistic-reprogramming-mechanisms
#8
Shao-Yao Ying, William Fang, Shi-Lung Lin
Pluripotency represents a unique feature of embryonic stem cells (ESCs). To generate ESC-like-induced pluripotent stem cells (iPSCs) derived from somatic cells, the cell genome needs to be reset and reprogrammed to express the ESC-specific transcriptome. Numerous studies have shown that genomic DNA demethylation is required for epigenetic reprogramming of somatic cell nuclei to form iPSCs; yet, the mechanism remains largely unclear. In ESCs, the reprogramming process goes through two critical stages: germline and zygotic demethylation, both of which erase genomic DNA methylation sites and hence allow for different gene expression patterns to be reset into a pluripotent state...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435940/mechanism-and-method-for-generating-tumor-free-ips-cells-using-intronic-microrna-mir-302-induction
#9
Shi-Lung Lin, Shao-Yao Ying
Today's researchers generating induced pluripotent stem cells (iPS cells or iPSCs) usually consider their pluripotency rather than potential tumorigenicity. Oncogenic factors such as c-Myc and Klf4 are frequently used to boost the survival and proliferative rates of iPSCs, creating an inevitable problem of tumorigenicity that hinders the therapeutic usefulness of these iPSCs. To prevent stem cell tumorigenicity, we have examined mechanisms by which the cell cycle genes are regulated in embryonic stem cells (ESCs)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435938/transgene-like-animal-models-using-intronic-micrornas
#10
Shi-Lung Lin, Shin-Ju E Chang, Shao-Yao Ying
Transgenic animal models are valuable tools for testing gene functions and drug mechanisms in vivo. They are also the best similitude for a human body for etiological and pathological research of diseases. All pharmaceutically developed medicines must be proven to be safe and effective in animals before approval by the Food and Drug Administration (FDA) to be used in clinical trials. To this end, the transgenic animal models of diseases serve as the front line of drug evaluation. However, there is currently no transgenic animal model for microRNA (miRNA)-related research...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435932/isolation-and-identification-of-gene-specific-micrornas
#11
Shi-Lung Lin, Donald C Chang, Shao-Yao Ying
Computer programming has identified hundreds of genomic hairpin sequences, many with functions yet to be determined. Because transfection of hairpin-like microRNA precursors (pre-miRNAs) into mammalian cells is not always sufficient to trigger RNA-induced gene silencing complex (RISC) assembly, a key step for inducing RNA interference (RNAi)-related gene silencing, we have developed an intronic miRNA expression system to overcome this problem by inserting a hairpin-like pre-miRNA structure into the intron region of a gene, and hence successfully increase the efficiency and effectiveness of miRNA-associated RNAi induction in vitro and in vivo...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435931/in-vitro-methods-for-analyzing-mirna-roles-in-cancer-cell-proliferation-invasion-and-metastasis
#12
Jian Xu, Xuelian Xiao, Daheng Yang
MicroRNAs (miRNAs) are small noncoding, single-stranded RNAs consisting of 20-24 nucleotides (Bartel, Cell 116:281-297, 2004), which regulate target genes expression by interacting with 3'-untranslated regions (3'-UTRs) of target mRNAs, leading to translation repression or mRNA degradation (Filipowicz et al., Nat Rev Genet 9:102-114, 2008; Nilsen, Trends Genet 23:243-249, 2007). Accumulating evidence has elucidated them as oncogenes or tumor suppressors in cancers such as hepatocellular carcinoma, breast cancer, and lung cancer (Liu et al...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435927/gene-silencing-in-vitro-and-in-vivo-using-intronic-micrornas
#13
Shi-Lung Lin, Shao-Yao Ying
MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering with intracellular messenger RNAs (mRNAs) that contain either complete or partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. Numerous miRNAs have been reported to induce RNA interference (RNAi), a post-transcriptional gene-silencing mechanism. Recent evidence also indicates that they are involved in the transcriptional regulation of genome activities. They were first discovered in Caenorhabditis elegans as native RNA fragments that modulate a wide range of genetic regulatory pathways during embryonic development, and are now recognized as small gene silencers transcribed from the noncoding regions of a genome...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435919/the-microrna
#14
Shao-Yao Ying, Donald C Chang, Shi-Lung Lin
MicroRNAs (miRNAs), widely distributed, small regulatory RNA genes, target both messenger RNA (mRNA) degradation and suppression of protein translation based on sequence complementarity between the miRNA and its targeted mRNA. Different names have been used to describe various types of miRNA. During evolution, RNA retroviruses or transgenes invaded the eukaryotic genome and were inserted itself in the noncoding regions of DNA, conceivably acting as transposon-like jumping genes, providing defense from viral invasion and fine-tuning of gene expression as a secondary level of gene modulation in eukaryotes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435152/mir-30a-suppresses-lung-cancer-progression-by-targeting-sirt1
#15
Yaowu Guan, Zhongming Rao, Cheng Chen
The class III histone deacetylase silent information regulator 1 (SIRT1) is frequently overexpressed in a variety of tumors, including lung cancer; however, its regulatory mechanisms are largely unknown. In this study, we found that an inconsistent trend between SIRT1 protein and mRNA levels in human lung cancer tissues, suggesting that a post-transcriptional mechanism may involved in SIRT1 regulation. Because microRNAs are important post-transcriptional regulators of gene expression, candidate miRNAs that could potentially bind SIRT1 were gained through bioinformatics analyses...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29435111/impact-of-dlk1-dio3-imprinted-cluster-hypomethylation-in-smoker-patients-with-lung-cancer
#16
Sonia Molina-Pinelo, Ana Salinas, Nicolás Moreno-Mata, Irene Ferrer, Rocío Suarez, Eduardo Andrés-León, Manuel Rodríguez-Paredes, Julian Gutekunst, Eloisa Jantus-Lewintre, Carlos Camps, Amancio Carnero, Luis Paz-Ares
DNA methylation is important for gene expression and genome stability, and its disruption is thought to play a key role in the initiation and progression of cancer and other diseases. The DLK1-DIO3 cluster has been shown to be imprinted in humans, and some of its components are relevant to diverse pathological processes. The purpose of this study was to assess the methylation patterns of the DLK1-DIO3 cluster in patients with lung cancer to study its relevance in the pathogenesis of this disease. We found a characteristic methylation pattern of this cluster in smoking associated lung cancer, as compared to normal lung tissue...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29434998/micrornas-and-cancer-key-paradigms-in-molecular-therapy
#17
Weige Tan, Bodu Liu, Shaohua Qu, Gehao Liang, Wei Luo, Chang Gong
MicroRNAs (miRNAs) are a type of small non-coding RNA molecule that performs an important role in post-transcriptional gene regulation. Since miRNAs were first identified in 1993, a number of studies have demonstrated that they act as tumor suppressors or oncogenes in human cancer, including colorectal, lung, brain, breast and liver cancer, and leukemia. Large high-throughput studies have previously revealed that miRNA profiling is critical for the diagnosis and prognosis of patients with cancer, while certain miRNAs possess the potential to be used as diagnostic and prognostic biomarkers or therapeutic targets in cancer...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434994/microrna-122-3p-inhibits-tumor-cell-proliferation-and-induces-apoptosis-by-targeting-forkhead-box-o-in-a549-cells
#18
Wen Wang, Jinsong Yang, Fenglei Yu, Wenjie Li, Li Wang, Haoyu Zou, Xia Long
The imbalance between cell proliferation and apoptosis was implicated to serve key roles in cancer pathogenesis. The characteristics of microRNAs (miRNAs/miRs) have attracted much attention in research focusing on cancer pathogenesis in recent years. miR-122-3p has been reported to be associated with a number of disease processes and pathogenesis, including lung cancer. The present study aimed to investigate the association of miR-122-3p expression level with cell proliferation and apoptosis in a lung cancer cell line...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434826/microrna-584-inhibits-cell-proliferation-and-invasion-in-non-small-cell-lung-cancer-by-directly-targeting-mtdh
#19
Yixiang Zhang, Yanjun Wang, Jinguang Wang
Lung cancer is the third most frequent human malignant tumour and the leading cause of cancer-associated mortality worldwide. Emerging lines of evidence have demonstrated that microRNAs (miRNAs) are upregulated or downregulated in non-small cell lung cancer (NSCLC), and this phenomenon is involved in the regulation of various processes during tumorigenesis and progression, including tumour groWTh, apoptosis, cell invasion, and tumour metastasis. Therefore, understanding the molecular mechanism that associates abnormally expressed miRNAs with NSCLC formation and development may lead to the identification of novel diagnostic, and therapeutic targets for patients with NSCLC...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29432024/evaluation-of-muc-1-aptamer-functionalized-hybrid-nanoparticles-for-targeted-delivery-of-mirna-29b-to-non-small-cell-lung-cancer
#20
Maryna Perepelyuk, Koita Sacko, Karthik Thangavel, Sunday A Shoyele
The objective of this study was to evaluate the therapeutic efficacy and pharmacokinetic study of mucin1-aptamer functionalized miRNA-29b-loaded hybrid nanoparticles (MAFMILHN) in lung tumor-bearing SCID mice. MAFMILHN were manufactured using an iso-electric point based nanotechnology. They were then fully characterized for particle size, loading capacity, Zeta potential and encapsulation efficiency. The ability of MAFMILHN to downregulate oncoprotein DNMT3B both at the cellular level and in vivo was monitored using western blot, while the effect of the downregulation of DNMT3B on tumor growth was assessed using bioluminescence...
February 12, 2018: Molecular Pharmaceutics
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