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https://www.readbyqxmd.com/read/28320202/identification-and-expression-of-equine-mer-derived-mirnas
#1
Jeong-An Gim, Heui-Soo Kim
MicroRNAs (miRNAs) are single-stranded, small RNAs (21-23 nucleotides) that function in gene silencing and translational inhibition via the RNA interference mechanism. Most miRNAs originate from host genomic regions, such as intergenic regions, introns, exons, and transposable elements (TEs). Here, we focused on the palindromic structure of medium reiteration frequencies (MERs), which are similar to precursor miRNAs. Five MER consensus sequences (MER5A1, MER53, MER81, MER91C, and MER117) were matched with paralogous transcripts predicted to be precursor miRNAs in the horse genome (equCab2) and located in either intergenic regions or introns...
March 21, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28319071/xrn2-promotes-emt-and-metastasis-through-regulating-maturation-of-mir-10a
#2
H Zhang, Y Lu, E Chen, X Li, B Lv, H G Vikis, P Liu
MicroRNAs (miRNAs) have been proposed as critical regulatory molecules in the epithelial-mesenchymal transition (EMT) program. However, the roles of mature miRNA biogenesis during EMT process needs to be defined. Here we determined that increased expression of XRN2 induced EMT and promoted metastasis in vitro and in vivo. Furthermore, we uncovered that XRN2 functions as pro-metastatic gene, which accelerates miR-10a maturation by binding pre-miR-10a in a DICER-independent manner. These findings suggest that XRN2 is a novel regulator of EMT that contributes to the metastatic processes in lung cancer through a novel miRNA regulatory mechanism...
March 20, 2017: Oncogene
https://www.readbyqxmd.com/read/28303893/changes-in-mirna-in-the-lung-and-whole-blood-after-whole-thorax-irradiation-in-rats
#3
Feng Gao, Pengyuan Liu, Jayashree Narayanan, Meiying Yang, Brian L Fish, Yong Liu, Mingyu Liang, Elizabeth R Jacobs, Meetha Medhora
We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28302568/mutational-profile-from-targeted-ngs-predicts-survival-in-ldct-screening-detected-lung-cancers
#4
Carla Verri, Cristina Borzi, Todd Holscher, Matteo Dugo, Andrea Devecchi, Katherine Drake, Stefano Sestini, Paola Suatoni, Elisa Romeo, Gabriella Sozzi, Ugo Pastorino, Mattia Boeri
BACKGROUND: The issue of overdiagnosis in low-dose computed tomography (LDCT)-screening trials could be addressed by the development of complementary biomarkers able to improve detection of aggressive disease. The mutation profile of LDCT screening-detected lung tumours is currently unknown. METHODS: Targeted next-generation sequencing was performed in 94 LDCT screening-detected lung tumours. Associations with clinicopathologic features, survival and the risk profile of a plasma microRNA signature classifier (MSC) were analyzed...
March 13, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28299977/identification-of-lymph-node-metastasis-related-micrornas-in-lung-adenocarcinoma-and-analysis-of-the-underlying-mechanisms-using-a-bioinformatics-approach
#5
Li Yan, Demin Jiao, Huizhen Hu, Jian Wang, Xiali Tang, Jun Chen, Qingyong Chen
This study aimed to screen lymphatic metastasis-related microRNAs (miRNAs) in lung adenocarcinoma and explore their underlying mechanisms using bioinformatics. The miRNA expression in primary lung adenocarcinoma, matched adjacent non-tumorigenic and lymph node metastasis tissues of patients were profiled via microarray. The screened metastasis-related miRNAs were then validated using quantitative real-time PCR in a second cohort of lung adenocarcinoma patients with lymphatic metastasis. Significance was determined using a paired t-test...
April 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28289824/a-mirna-signature-for-an-environmental-heterocyclic-amine-defined-by-a-multi-organ-carcinogenicity-bioassay-in-the-rat
#6
Ying-Shiuan Chen, Rong Wang, Wan-Mohaiza Dashwood, Christiane V Löhr, David E Williams, Emily Ho, Susanne Mertens-Talcott, Roderick H Dashwood
Heterocyclic amines (HCAs) produced during high-temperature cooking have been studied extensively in terms of their genotoxic/genetic effects, but recent work has implicated epigenetic mechanisms involving non-coding RNAs. Colon tumors induced in the rat by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) have altered microRNA (miRNA) signatures linked to dysregulated pluripotency factors, such as c-Myc and Krüppel-like factor 4 (KLF4). We tested the hypothesis that dysregulated miRNAs from PhIP-induced colon tumors would provide a "PhIP signature" for use in other target organs obtained from a 1-year carcinogenicity bioassay in the rat...
March 13, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28288782/exosomes-a-new-horizon-in-lung-cancer
#7
REVIEW
Irene Vanni, Angela Alama, Francesco Grossi, Maria Giovanna Dal Bello, Simona Coco
Circulating exosomes are the major mediators of cell-cell communication. They have been found in various body fluids of healthy individuals and patients with malignancies as cargos of several molecules including miRNAs. Several studies have underlined the role of exosome miRNAs in different tumor types, including lung cancer, suggesting their potential use as biomarkers and therapeutic agents. An overview of the biology and function of exosomes and exosome miRNAs as indicators of diagnosis and treatment response in lung cancer is presented...
March 10, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28280736/microrna-related-polymorphisms-in-pi3k-akt-mtor-pathway-genes-are-predictive-of-limited-disease-small-cell-lung-cancer-treatment-outcomes
#8
Wei Jiang, Wenjue Zhang, Lihong Wu, Lipin Liu, Yu Men, Jingbo Wang, Jun Liang, Zhouguang Hui, Zongmei Zhou, Nan Bi, Luhua Wang
The phosphoinositide-3 kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway plays an important role in cancer progression and treatment, including that of small cell lung cancer (SCLC), a disease with traditionally poor prognosis. Given the regulatory role of microRNA (miRNA) in gene expression, we examined the association of single nucleotide polymorphisms (SNPs) at miRNA-binding sites of genes in the mTOR pathway with the prognosis of patients with limited-disease SCLC. A retrospective study was conducted of 146 patients with limited-disease SCLC treated with chemoradiotherapy...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28275243/the-microrna-expression-signature-of-small-cell-lung-cancer-tumor-suppressors-of-mir-27a-5p-and-mir-34b-3p-and-their-targeted-oncogenes
#9
Keiko Mizuno, Hiroko Mataki, Takayuki Arai, Atsushi Okato, Kazuto Kamikawaji, Tomohiro Kumamoto, Tsubasa Hiraki, Kazuhito Hatanaka, Hiromasa Inoue, Naohiko Seki
Small cell lung cancer (SCLC) constitutes approximately 15% of all diagnosed lung cancers. SCLC is a particularly lethal malignancy, as the 2-year survival rate after appropriate treatment is less than 5%. The patients with SCLC have not been received a benefit of the recently developed molecular targeted treatment. Therefore, a new treatment strategy is necessary for the patients. The molecular mechanisms underlying the aggressiveness of SCLC cells and their development of treatment-resistance are still ambiguous...
March 9, 2017: Journal of Human Genetics
https://www.readbyqxmd.com/read/28274991/macrophage-derived-apoptotic-bodies-promote-the-proliferation-of-the-recipient-cells-via-shuttling-microrna-221-222
#10
Ziwen Zhu, Duo Zhang, Heedoo Lee, Aravind Ajakumar Menon, Jingxuan Wu, Kebin Hu, Yang Jin
Bacterial pneumonia is a common and serious clinical entity. Alveolar epithelial cells and alveolar macrophages are the first line of defense in the innate immunity against bacterial pathogens. Epithelial cells are known to release chemokines/cytokines that recruit and activate phagocytic cells. However, the signals sent from alveolar macrophages back to the lung epithelial cells remain largely unexplored. We found that LPS, a well-recognized stimulator derived from gram-negative (G(-)) bacteria, rapidly and robustly induces the secretion of macrophage-derived extracellular vesicles (EVs)...
March 8, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28272712/the-expression-and-function-of-mirna-106-in-pediatric-osteosarcoma
#11
M Xu, Y-Y Zhang, H-F Wang, G-S Yang
OBJECTIVE: This study is to investigate the expression and biological function of miRNA-106b in osteosarcoma. PATIENTS AND METHODS: Freshly resected osteosarcoma tissues and the corresponding para-carcinoma tissues were collected from 54 patients. Then miR-106b level in the carcinoma tissues was detected by real-time PCR. To test the function of miR-106b, osteosarcoma cell line U2-OS was transfected with miR-106b inhibitor in vitro. Then, the proliferation, cell cycle, invasion and metastasis of U2-OS cells were detected by CCK-8 assay, flow cytometry, and transwell respectively...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28266708/role-of-wnt-%C3%AE-catenin-signaling-regulatory-micrornas-in-the-pathogenesis-of-colorectal-cancer
#12
REVIEW
Farzad Rahmani, Amir Avan, Seyed Isaac Hashemy, Seyed Mahdi Hassanian
Colorectal cancer (CRC) is one of the leading causes of cancer death worldwide. In more than 90% of all CRC patients, the master oncogenic Ras-Wnt signaling axis is over-activated. MicroRNAs (miRNAs) are potential novel diagnostic and prognostic biomarkers as well as therapeutic targets for several cancers including lung, breast, gastric and colorectal cancers. Oncogenic or tumor suppressor miRNAs modulate tumor cells proliferation, cell cycle progression, angiogenesis, invasion and metastasis through regulating oncogenic pathways including Wnt/β-catenin signaling...
March 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28262617/microrna-23b-suppresses-epithelial-mesenchymal-transition-emt-and-metastasis-in-hepatocellular-carcinoma-via-targeting-pyk2
#13
Jie Cao, Jinkai Liu, Jianyun Long, Jing Fu, Liang Huang, Jing Li, Caifeng Liu, Xianghua Zhang, Yiqun Yan
Numerous microRNAs (miRNAs) have been shown to play important roles in various cancers, including hepatocellular carcinoma (HCC). However, the functions and mechanisms of the miRNAs involved in HCC progress and metastasis still remain unknown. We downloaded the normalized data of microRNA expression profiling of HCC comparing primary tumor with lung metastasis from GEO database (GSE26323), and gain a group of metastasis-related candidate miRNAs. Among the candidate miRNAs, we focused on miR-23b for further study...
March 2, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28260111/molecular-characterization-of-metastatic-osteosarcoma-differentially-expressed-genes-transcription-factors-and-micrornas
#14
Lisong Heng, Zhen Jia, Jie Bai, Kun Zhang, Yangjun Zhu, Jianbing Ma, Jun Zhang, Honghao Duan
The present study aimed to understand the molecular mechanisms underlying osteosarcoma metastasis. Microarray dataset GSE49003 was downloaded from the Gene Expression Omnibus database and used for analysis. Raw expression data were preprocessed using the preprocessCore, impute and aggregate packages in R. Differentially expressed genes (DEGs) between metastatic and non‑metastatic osteosarcoma cell lines were screened using the limma package following exclusion of DEGs with a higher significance in intra‑groups compared with inter‑groups using the genefilter package...
March 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28260088/foxk2-inhibits-non-small-cell-lung-cancer-epithelial-mesenchymal-transition-and-proliferation-through-the-repression-of-different-key-target-genes
#15
Shu Chen, Simin Jiang, Fen Hu, Yongjian Xu, Tao Wang, Qi Mei
Increasing evidence suggests that numerous fork-head transcription factors are required to repress the mammalian cells phenotype. Among them, Foxk2 is a ubiquitously expressed family member, but the role of Foxk2 in mediating tumor metastasis in non-small cell lung cancer has not been explored. In this investigation reduced Foxk2 expression was found in lung adenocarcinoma tissues compared with the adjacent non-tumor tissues, and was associated with better overall survival. Low expression was also found in the NSCLC cell lines such as A549, NCI-H520, H1299, H358 and H460 cells...
February 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28259929/expression-profiles-and-circulation-dynamics-of-rat-mesenteric-lymph-micrornas
#16
Wakako Sakamoto, Tomohiko Masuno, Hiroyuki Yokota, Toshihiro Takizawa
Mesenteric lymph is vital for immune cell trafficking and intestinal fluid and chyle transport, which aid homeostatic maintenance. There have been few reports investigating the profiles and circulatory dynamics of mesenteric lymph microRNAs (miRNAs). The present study aimed to provide a comprehensive analysis of miRNAs in normal rodent mesenteric lymph. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR)‑based array analysis was performed to examine the expression levels of 375 miRNAs in normal rat mesenteric lymph...
February 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28255128/-recent-advances-in-mir-200c-and-fibrosis-in-organs
#17
Jun Chen, Jinyu Cai, Cui Du, Qiong Cao, Min Li, Binjie Liu
In the fibrosis and pterygium of lung, liver, kidney, peritoneum or skin, miR-200c was aberrantly expressed. It has been shown that the regulatory effect of miR-200c on fibrosis in organ was involved in TGF-β-mediated epithelial-mesenchymal transition. The abnormal level of miR-200c in serum may be a basis for early diagnosis of lung fibrosis. Furthermore, miRNA mimics, miRNA agomir, and miRNA inhibitor are potential therapeutic tools for fibrosis. In present review, we summarize the recent progress in relevant studies on the expression and regulatory function of miR-200c and focus on its role in diagnosis, treatment, and prognosis of fibrosis in organ...
February 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28249921/mir-20b-induced-increase-in-myeloid-derived-suppressor-cells-in-the-lungs-of-mice-with-chronic-asthma
#18
Hua Ma, Helong Wang, Yulan Luo, Shujun Guo, Chuanwang Song
Bronchial asthma is a common chronic airway inflammatory disease. MicroRNAs (miRNAs) play an important role in the pathogenesis of asthma. We have previously shown that miR-20b can inhibit airway inflammation in asthmatic mice, but the exact mechanism is unknown. In the present study, we show that administration of nasal drops containing miR-20b induced an increase in the percentage of Gr1(+)CD11b(+) myeloid-derived suppressor cells (MDSCs) in lung tissue from asthmatic mice, and the content of TGF-β in lung tissues also increased after treatment...
January 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28248928/the-lncrna-xist-exhibits-oncogenic-properties-via-regulation-of-mir-449a-and-bcl-2-in-human-non-small-cell-lung-cancerthis-article-has-been-corrected-since-advanced-online-publication-and-an-erratum-is-also-printed-in-this-issue
#19
Ya-Long Zhang, Xue-Bing Li, Yan-Xu Hou, Nian-Zhen Fang, Jia-Cong You, Qing-Hua Zhou
Long non-coding RNAs (lncRNAs) are associated with the occurrence, development and prognoses of non-small cell lung cancer (NSCLC). In the present study, we investigated the functional mechanisms of the lncRNA XIST in two human NSCLC cell lines, A549 and NCI-H1299. In all the 5 NSCLC cell lines (NL9980, NCI-H1299, NCI-H460, SPC-A-1 and A549) tested, the expression levels of XIST were significantly elevated, as compared with those in normal human bronchial epithelial cell line BEAS-2B. In A549 and NCI-H1299 cells, knockdown of XIST by siRNA significantly inhibited the cell proliferation, migration and invasion, and promoted cell apoptosis...
March 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28247764/application-of-spectral-crosstalk-correction-for-improving-multiplexed-microrna-detection-using-a-single-excitation-wavelength
#20
Yuanjian Liu, Min Wei, Ying Li, Anran Liu, Wei Wei, Yuanjian Zhang, Songqin Liu
MicroRNAs (miRNAs) play crucial roles in the regulation of cellular activities and are next-generation biomarkers for early cancer detection. Simultaneous monitoring of multiplexed miRNA is very important for enhancing the accuracy of cancer diagnostics. Traditional fluorescence methods for multicomponent analysis were usually operated under multiple excitation wavelengths, because spectral crosstalk is very detrimental to detecting accuracy for multicomponent analysis. Herein, we present a fluorescence strategy for multi-miRNAs detection in plasma under a single excitation wavelength...
March 10, 2017: Analytical Chemistry
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