Maria Alieva, Mario Barrera Román, Sam de Blank, Diana Petcu, Amber L Zeeman, Noël M M Dautzenberg, Annelisa M Cornel, Cesca van de Ven, Rob Pieters, Monique L den Boer, Stefan Nierkens, Friso G J Calkoen, Hans Clevers, Jürgen Kuball, Zsolt Sebestyén, Ellen J Wehrens, Johanna F Dekkers, Anne C Rios
Modeling immuno-oncology by using patient-derived material and immune cell co-cultures can advance our understanding of immune cell tumor targeting in a patient-specific manner, offering leads to improve cellular immunotherapy. However, fully exploiting these living cultures requires analysis of the dynamic cellular features modeled, for which protocols are currently limited. Here, we describe the application of BEHAV3D, a platform that implements multi-color live 3D imaging and computational tools for: (i) analyzing tumor death dynamics at both single-organoid or cell and population levels, (ii) classifying T cell behavior and (iii) producing data-informed 3D images and videos for visual inspection and further insight into obtained results...
March 19, 2024: Nature Protocols