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https://www.readbyqxmd.com/read/28813164/pembrolizumab-in-patients-with-extensive-stage-small-cell-lung-cancer-results-from-the-phase-ib-keynote-028-study
#1
Patrick A Ott, Elena Elez, Sandrine Hiret, Dong-Wan Kim, Anne Morosky, Sanatan Saraf, Bilal Piperdi, Janice M Mehnert
Purpose The safety and efficacy of pembrolizumab, a humanized monoclonal antibody against programmed death 1 (PD-1), were assessed in patients with programmed death ligand 1 (PD-L1)-expressing extensive-stage small-cell lung cancer (SCLC) in the multicohort, phase Ib open-label KEYNOTE-028 study ( ClinicalTrials.gov identifier: NCT02054806). Methods Patients with SCLC received pembrolizumab 10 mg/kg every 2 weeks for 24 months or until disease progression or intolerable toxicity occurred. PD-L1 expression was assessed by immunohistochemistry...
August 16, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28812987/a-microneedle-functionalized-with-polyethyleneimine-and-nanotubes-for-highly-sensitive-label-free-quantification-of-dna
#2
Darius Saadat-Moghaddam, Jong-Hoon Kim
The accurate measure of DNA concentration is necessary for many DNA-based biological applications. However, the current methods are limited in terms of sensitivity, reproducibility, human error, and contamination. Here, we present a microneedle functionalized with polyethyleneimine (PEI) and single-walled carbon nanotubes (SWCNTs) for the highly sensitive quantification of DNA. The microneedle was fabricated using ultraviolet (UV) lithography and anisotropic etching, and then functionalized with PEI and SWCNTs through a dip coating process...
August 16, 2017: Sensors
https://www.readbyqxmd.com/read/28812103/re-expression-of-pro-fibrotic-embryonic-preserved-mediators-in-irradiated-arterial-vessels-of-the-head-and-neck-region
#3
Patrick Möbius, Raimund H M Preidl, Manuel Weber, Kerstin Amann, Friedrich W Neukam, Falk Wehrhan
PURPOSE: Surgical treatment of head and neck malignancies frequently includes microvascular free tissue transfer. Preoperative radiotherapy increases postoperative fibrosis-related complications up to transplant loss. Fibrogenesis is associated with re-expression of embryonic preserved tissue developmental mediators: osteopontin (OPN), regulated by sex-determining region Y‑box 9 (Sox9), and homeobox A9 (HoxA9) play important roles in pathologic tissue remodeling and are upregulated in atherosclerotic vascular lesions; dickkopf-1 (DKK1) inhibits pro-fibrotic and atherogenic Wnt signaling...
August 15, 2017: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/28811965/adjuvant-immunotherapy-with-autologous-dendritic-cells-for-hepatocellular-carcinoma-randomized-phase-ii-study
#4
Jeong-Hoon Lee, Won Young Tak, Yoon Lee, Min-Kyu Heo, Jae-Sung Song, Hak-Yeop Kim, Soo Young Park, Si Hyun Bae, Joon Hyeok Lee, Jeong Heo, Ki-Hwan Kim, Yong-Soo Bae, Yoon Jun Kim
Our previous phase I/IIA study showed that autologous dendritic cells (DCs) pulsed with tumor-associated antigens are well tolerated in patients with hepatocellular carcinoma (HCC). In this randomized, multicenter, open-label, phase II trial, we investigated the efficacy and safety of this DC-based adjuvant immunotherapy with 156 patients, who treated for HCC with no evidence of residual tumor after standard treatment modalities. Patients were randomly assigned to immunotherapy (n = 77; injection of 3 × 10(7) DC cells, six times over 14 weeks) or control (n = 79; no treatment)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28811610/antibiotic-induced-release-of-small-extracellular-vesicles-exosomes-with-surface-associated-dna
#5
Andrea Németh, Norbert Orgovan, Barbara W Sódar, Xabier Osteikoetxea, Krisztina Pálóczi, Katalin É Szabó-Taylor, Krisztina V Vukman, Ágnes Kittel, Lilla Turiák, Zoltán Wiener, Sára Tóth, László Drahos, Károly Vékey, Robert Horvath, Edit I Buzás
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811551/nano-metamaterials-for-ultrasensitive-terahertz-biosensing
#6
Dong-Kyu Lee, Ji-Hun Kang, Junghoon Kwon, Jun-Seok Lee, Seok Lee, Deok Ha Woo, Jae Hun Kim, Chang-Seon Song, Q-Han Park, Minah Seo
As a candidate for a rapid detection of biomaterials, terahertz (THz) spectroscopy system can be considered with some advantage in non-destructive, label-free, and non-contact manner. Because protein-ligand binding energy is in the THz range, especially, most important conformational information in molecular interactions can be captured by THz electromagnetic wave. Based on the THz time-domain spectroscopy system, THz nano-metamaterial sensing chips were prepared for great enhancing of detection sensitivity...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811371/angular-reconstitution-based-3d-reconstructions-of-nanomolecular-structures-from-superresolution-light-microscopy-images
#7
Desirée Salas, Antoine Le Gall, Jean-Bernard Fiche, Alessandro Valeri, Yonggang Ke, Patrick Bron, Gaetan Bellot, Marcelo Nollmann
Superresolution light microscopy allows the imaging of labeled supramolecular assemblies at a resolution surpassing the classical diffraction limit. A serious limitation of the superresolution approach is sample heterogeneity and the stochastic character of the labeling procedure. To increase the reproducibility and the resolution of the superresolution results, we apply multivariate statistical analysis methods and 3D reconstruction approaches originally developed for cryogenic electron microscopy of single particles...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28810977/moving-beyond-accidental-leadership-a-graduate-medical-education-leadership-curriculum-needs-assessment
#8
Joshua D Hartzell, Clifton E Yu, Brian M Cohee, Michael R Nelson, Ramey L Wilson
BACKGROUND: Despite calls for greater physician leadership, few medical schools, and graduate medical education programs provide explicit training on the knowledge, skills, and attitudes necessary to be an effective physician leader. Rather, most leaders develop through what has been labeled "accidental leadership." A survey was conducted at Walter Reed to define the current status of leadership development and determine what learners and faculty perceived as key components of a leadership curriculum...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28810820/quantitative-label-free-evaluation-of-tissue-engineered-skeletal-muscle-through-multiphoton-microscopy
#9
Brian C Syverud, Mary-Ann Mycek, Lisa Marie Larkin
The lack of tools for assessing engineered tissue viability and function in a non-invasive manner is a major regulatory and translational challenge facing tissue engineers. Label-free, nonlinear optical molecular imaging (OMI) has utilized endogenous nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) fluorescence to indicate metabolic activity. Similarly, second harmonic generation (SHG) signals from myosin and collagen can measure overall muscle structural integrity and function...
August 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28810232/microfluidic-dielectrophoresis-device-for-trapping-counting-and-detecting-shewanella-oneidensis-at-the-cell-level
#10
Xiangyu Chen, Zhiting Liang, Daobo Li, Ying Xiong, Penghui Xiong, Yong Guan, Shuangyue Hou, Yue Hu, Shan Chen, Gang Liu, Yangchao Tian
Shewanella oneidensis, a model organism for electrochemical activity bacteria, has been widely studied at the biofilm level. However, to obtain more information regarding this species, it is essential to develop an approach to trap and detect S. oneidensis at the cell level. In this study, we report a rapid and label-free microfluidic platform for trapping, counting and detecting S. oneidensis cells. A microfluidic chip was integrated with a modified dielectrophoresis (DEP) trapping technique and hole arrays of different hole sizes...
August 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28809987/label-free-ferrohydrodynamic-cell-separation-of-circulating-tumor-cells
#11
Wujun Zhao, Rui Cheng, Brittany D Jenkins, Taotao Zhu, Nneoma E Okonkwo, Courtney E Jones, Melissa B Davis, Sravan K Kavuri, Zhonglin Hao, Carsten Schroeder, Leidong Mao
Circulating tumor cells (CTCs) have significant implications in both basic cancer research and clinical applications. To address the limited availability of viable CTCs for fundamental and clinical investigations, effective separation of extremely rare CTCs from blood is critical. Ferrohydrodynamic cell separation (FCS), a label-free method that conducted cell sorting based on cell size difference in biocompatible ferrofluids, has thus far not been able to enrich low-concentration CTCs from cancer patients' blood because of technical challenges associated with processing clinical samples...
August 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28809979/raman-and-infrared-spectroscopy-differentiate-senescent-from-proliferating-cells-in-a-human-dermal-fibroblast-3d-skin-model
#12
Katharina Eberhardt, Christian Matthäus, Doreen Winter, Cornelia Wiegand, Uta-Christina Hipler, Stephan Diekmann, Jürgen Popp
Senescent cells contribute to tissue aging and dysfunction. Therefore, detecting senescent cells in skin is of interest for skin tumor diagnostics and therapy. Here, we studied the transition into senescence of human dermal fibroblasts (HDFs) in a three-dimensional (3D) human fibroblast-derived matrix (FDM). Senescent and proliferating cells were imaged by Raman spectroscopy (RS) and Fourier transform infrared (FTIR) spectroscopy. The obtained averaged spectra were analyzed using PLS-LDA. For these 3D cultured cells, RS and FTIR could clearly distinguish senescent from proliferating cells...
August 15, 2017: Analyst
https://www.readbyqxmd.com/read/28809896/multifrequency-excitation-and-detection-scheme-in-apertureless-scattering-near-field-scanning-optical-microscopy
#13
H Greener, M Mrejen, U Arieli, H Suchowski
Near-field scanning optical microscopy has revolutionized the study of fundamental physics, as it is one of very few label-free optical noninvasive nanoscale-resolved imaging techniques. However, its resolution remains strongly limited by the poor discrimination of weak near-field optical signals from a far-field background. Here, we theoretically and experimentally demonstrate a multifrequency excitation and detection scheme in apertureless near-field optical microscopy that exceeds current state-of-the-art sensitivity and background suppression...
August 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28809678/ultrasound-aided-vertebral-level-localization-for-lumbar-surgery
#14
Nora Baka, Sieger Leenstra, Theo van Walsum
Localization of the correct vertebral level for surgical entry during lumbar hernia surgery is not straightforward. In this paper we develop and evaluate a solution using free-hand 2D ultrasound (US) imaging in the operation room (OR). Our system exploits the difference in spinous process shapes of the vertebrae. The spinous processes are pre-operatively outlined and labeled in a lateral lumbar X-ray of the patient. Then, in the OR the spinous processes are imaged with 2D sagittal US, and are automatically segmented and registered with the X-ray shapes...
August 10, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28809543/infrared-spectroscopy-coupled-with-a-dispersion-model-for-quantifying-the-real-time-dynamics-of-kanamycin-resistance-in-artificial-microbiota
#15
Naifu Jin, Maria Paraskevaidi, Kirk T Semple, Francis Luke Martin, Dayi Zhang
Over-usage of antibiotics leads to the widespread induction of antibiotic resistance genes (ARGs). Developing an approach to allow real-time monitoring and fast prediction of ARGs dynamics in clinical or environmental samples has become an urgent matter. Vibrational spectroscopy is potentially an ideal technique towards the characterization of the microbial composition of microbiota as it is non-destructive, high-throughput and label-free. Herein, we employed attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and developed a spectrochemical tool to quantify the static and dynamic composition of kanamycin resistance in artificial microbiota to evaluate microbial antibiotic resistance...
August 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28809425/ph-responsive-quencher-free-molecular-beacon-systems-containing-2-deoxyuridine-units-labeled-with-fluorene-derivatives
#16
Ji Won Lee, Ye-Seul Son, Jun Yeon Hwang, Yoojin Park, Gil Tae Hwang
Three fluorescent nucleosides-U(FL), U(AF), and U(DAF), containing fluorene, 2-aminofluorene, and 2-dimethylaminofluorene units, respectively, covalently attached to 2'-deoxyuridine-have been incorporated into the central positions of oligodeoxynucleotides (ODNs) to examine the effects of their flanking bases (FBs) and pH on the emission properties upon hybridization with fully matched and single-base-mismatched targets. The ODN containing U(FL) and cytosine-FBs in the pH range from 5.5 to 8.0 and the ODN containing U(DAF) and cytosine-FBs under slightly acidic conditions (pH 6...
August 15, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28809114/boolean-logic-tree-of-label-free-dual-signal-electrochemical-aptasensor-system-for-biosensing-three-state-logic-computation-and-keypad-lock-security-operation
#17
Jiao Yang Lu, Xin Xing Zhang, Wei Tao Huang, Qiu Yan Zhu, Xuezhi Ding, Liqiu Xia, Hong Qun Luo, Nian Bing Li
The most serious and yet unsolved problems of molecular logic computing, consist in how to connect molecular events in complex systems into a usable device with specific functions and how to selectively control branchy logic processes from the cascading logic systems. This report demonstrates that Boolean logic tree is utilized to organize and connect "plug and play" chemical events DNA, nanomaterials, organic dye, biomolecule, and denaturant for developing the dual-signal electrochemical evolution aptasensor system with good resettability for amplification detection of thrombin, controllable and selectable three-state logic computation and keypad lock security operation...
August 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28809078/profiling-protein-s-sulfination-with-maleimide-linked-probes
#18
Yu-Hsuan Kuo, Aaron M Konopko, Nicholas B Borotto, Jaimeen D Majmudar, Sarah E Haynes, Brent R Martin
Cysteine residues are susceptible to oxidation to form S-sulfinyl (R-SO2H) and S-sulfonyl (R-SO3H) post-translational modifications. Here we present a simple bioconjugation strategy to label S-sulfinated proteins using reporter-linked maleimides. After alkylation of free thiols with iodoacetamide, S-sulfinated cysteines react with maleimide to form a sulfone Michael adduct that remains stable under acidic conditions. Using this sequential alkylation strategy, we demonstrate differential S-sulfination across mouse tissue homogenates, as well as enhanced S-sulfination following pharmacological induction of ER stress, lipopolysaccharide stimulation, and inhibitors of the electron transport chain...
August 14, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28808224/off-label-use-of-crizotinib-as-a-neoadjuvant-treatment-for-a-young-patient-when-conventional-chemotherapy-gave-no-benefits-in-stage-iiia-non-small-cell-lung-cancer
#19
Delphine Dumont, Pascal Dô, Delphine Lerouge, Gaëtane Planchard, Marc Riffet, Catherine Dubos-Arvis, Serge Danhier, Radj Gervais
BACKGROUND The treatment of locally advanced non-small cell lung cancer involves a combination of chemotherapy, surgery, and radiotherapy. Each case is discussed and the best strategy is chosen individually, following international guidelines. CASE REPORT A 37-year-old man was diagnosed with locally advanced broncho-pulmonary adenocarcinoma (stage IIIA). The disease was stable after 2 cycles of cisplatin plus Navelbine used as neoadjuvant therapy. FISH analysis revealed an ALK rearrangement. The patient then received unlicensed crizotinib as second-line neoadjuvant treatment, which led to an almost complete radiological and metabolic response...
August 15, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28807037/selepressin-a-novel-selective-vasopressin-v1a-agonist-is-an-effective-substitute-for-norepinephrine-in-a-phase-iia-randomized-placebo-controlled-trial-in-septic-shock-patients
#20
James A Russell, Jean-Louis Vincent, Anne Louise Kjølbye, Håkan Olsson, Allan Blemings, Herbert Spapen, Peder Carl, Pierre-Francois Laterre, Lars Grundemar
BACKGROUND: Vasopressin is widely used for vasopressor support in septic shock patients, but experimental evidence suggests that selective V1A agonists are superior. The initial pharmacodynamic effects, pharmacokinetics, and safety of selepressin, a novel V1A-selective vasopressin analogue, was examined in a phase IIa trial in septic shock patients. METHODS: This was a randomized, double-blind, placebo-controlled multicenter trial in 53 patients in early septic shock (aged ≥18 years, fluid resuscitation, requiring vasopressor support) who received selepressin 1...
August 15, 2017: Critical Care: the Official Journal of the Critical Care Forum
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