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https://www.readbyqxmd.com/read/29334610/phase-ii-study-of-dovitinib-in-patients-with-castration-resistant-prostate-cancer-kcsg-gu11-05
#1
Yoon Ji Choi, Hye Sook Kim, Se Hoon Park, Bong-Seog Kim, Kyoung Ha Kim, Hyo Jin Lee, Hong Suk Song, Dong-Yeop Shin, Ha Young Lee, Hoon-Gu Kim, Kyung Hee Lee, Jae Lyun Lee, Kyong Hwa Park
Purpose: Fibroblast growth factor (FGF) signals are important in carcinogenesis and progression of prostate cancer. Dovitinib is an oral, pan-class inhibitor of vascular endothelial growth factor receptor (VEGFR), platelet-derived growth factor receptor, and fibroblast growth factor receptor (FGFR). We evaluated the efficacy and toxicity of dovitinib in men with metastatic castration resistant prostate cancer (mCRPC). Materials and Methods: This study was a single-arm, phase II, open-label, multicenter trial of dovitinib 500mg/day (5-days-on/2-days-off schedule)...
January 2, 2018: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29334115/label-free-multidimensional-information-acquisition-from-optogenetically-engineered-cells-using-a-graphene-transistor
#2
Gongxin Li, Jia Yang, Wenguang Yang, Feifei Wang, Yuechao Wang, Wenxue Wang, Lianqing Liu
The optogenetic technique, which allows the manipulation of cellular activity patterns in space and time by light, has transformed the field of neuroscience. However, acquiring multidimensional optogenetic information remains challenging despite the fact that several cellular information detection methods have been proposed. Herein, we present a new method to acquire label-free multidimensional information from optogenetically engineered cells using a graphene transistor. Using a graphene film to form a strong densely packed layer with cells, the cellular action potentials were characterized as light-activated transistor conductance signals, which quantified the multidimensional optogenetic information...
January 15, 2018: Nanoscale
https://www.readbyqxmd.com/read/29333852/synthesis-of-mip-nanoparticles-for-%C3%AE-casein-detection-using-spr-as-a-milk-allergen-sensor
#3
Jon Ashley, Yunus Shukor, Roberta D'Aurelio, Linda Trinh, Thomas Lawrence Rodgers, Jeff Temblay, Mike Pleasants, Ibtisam E Tothill
Food recalls due to undeclared allergens or contamination are costly to the food manufacturing industry worldwide. As the industry strives for better manufacturing efficiencies over a diverse range of food products, there is a need for the development of new analytical techniques to improve monitoring the presence of unintended food allergens during the food manufacturing process. In particular, the monitoring of wash samples from cleaning in place systems (CIP), used in the cleaning of food processing equipment, would allow for the effective removal of allergen containing ingredients in between food batches...
January 15, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29333575/a-phase-1-dose-escalation-study-of-pf-06664178-an-anti-trop-2-aur0101-antibody-drug-conjugate-in-patients-with-advanced-or-metastatic-solid-tumors
#4
Gentry T King, Keith D Eaton, Brandon R Beagle, Christopher J Zopf, Gilbert Y Wong, Heike I Krupka, Steven Y Hua, Wells A Messersmith, Anthony B El-Khoueiry
Purpose and Methods Trop-2 is a glycoprotein over-expressed in many solid tumors but at low levels in normal human tissue, providing a potential therapeutic target. We conducted a phase 1 dose-finding study of PF-06664178, an antibody-drug conjugate that targets Trop-2 for the selective delivery of the cytotoxic payload Aur0101. The primary objective was to determine the maximum tolerated dose and recommended phase 2 dose. Secondary objectives included further characterization of the safety profile, pharmacokinetics and antitumor activity...
January 15, 2018: Investigational New Drugs
https://www.readbyqxmd.com/read/29333005/prognostic-factors-and-survival-outcomes-of-intracranial-ependymoma-treated-with-multimodality-approach
#5
Kunhi Parambath Haresh, Ajeet Kumar Gandhi, Supriya Mallick, Rony Benson, Subhash Gupta, Daya Nand Sharma, Pramod Kumar Julka, Goura Kisor Rath
Objectives: We aimed to analyze treatment outcomes of intracranial ependymoma (ICE) treated at our institute with multimodality approach. Materials and Methods: Demography, treatment details, and survival data of 40 patients (2005-2012) were collected in a predesigned pro forma. Kaplan Meier method was used to analyze disease-free survival (DFS) and the impact of prognostic factors was determined using univariate analysis (log-rank test). Multivariate analysis was performed using Cox-proportional hazard model...
October 2017: Indian Journal of Medical and Paediatric Oncology
https://www.readbyqxmd.com/read/29332793/label-free-detection-of-histone-based-on-cationic-conjugated-polymer-mediated-fluorescence-resonance-energy-transfer
#6
Xiaozhen Lu, Hongxia Jia, Xiaohua Yan, Jingsha Wang, Yucong Wang, Chenghui Liu
A simple and homogeneous histone assay is developed based on histone-induced DNA compressing coupled with cationic conjugated polymer (CCP)-mediated fluorescence resonance energy transfer (FRET). In this strategy, the CCP serves as the FRET donor and SYBR Green I (SG), which can strongly fluoresce not at its free state but after intercalated into the double stranded calf thymus DNA (dsDNA), serves as the acceptor of FRET. In the absence of histone, the dsDNA-SG and CCP combine with each other through electrostatic interaction and the strong FRET from CCP to SG occurs due to the overlapping between the fluorescent emitting spectrum of the CCP and the absorption spectrum of SG...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332431/validation-of-the-ceredigion-youth-screening-tool
#7
Gareth Norris, Gwyn Griffith, Megan West
Evidence suggests that only a small minority of youth offenders will continue their behaviour in the longer term and largely independent of any interventions they may receive (Bateman, 2011; Haines & Case, 2015). Hence, "screening out" this larger low-risk cohort could have a positive impact upon the individual through a reduction in stigmatisation/labelling and free up resources for higher risk clients. This article outlines development of the Ceredigion Youth Screening Tool (CYSTEM)-developed and tested to address the two facets of criminality and vulnerability-closely aligned to the eight key risk indicators identified in the Risk-Needs-Responsivity (R-N-R) literature (Andrews & Bonta, 2010)...
January 1, 2018: International Journal of Offender Therapy and Comparative Criminology
https://www.readbyqxmd.com/read/29332401/proteomic-profiling-of-leishmania-donovani-promastigote-subcellular-organelles
#8
Armando Jardim, Darryl B Hardie, Jan Boitz, Christoph H Borchers
To facilitate a greater understanding of the biological processes in the medically important Leishmania donovani parasite, a combination of differential and density-gradient ultracentrifugation techniques were used to achieve a comprehensive subcellular fractionation of the promastigote stage. An in-depth label-free proteomic LC-MS/MS analysis of the density gradients resulted in the identification of ~50% of the Leishmania proteome (3883 proteins detected), which included ~645 integral membrane proteins and 1737 uncharacterized proteins...
January 15, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29332135/sequential-versus-simultaneous-use-of-chemotherapy-and-gonadotropin-releasing-hormone-agonist-gnrha-among-estrogen-receptor-er-positive-premenopausal-breast-cancer-patients-effects-on-ovarian-function-disease-free-survival-and-overall-survival
#9
Ying Zhang, Yajie Ji, Jianwei Li, Li Lei, Siyu Wu, Wenjia Zuo, Xiaoqing Jia, Yujie Wang, Miao Mo, Na Zhang, Zhenzhou Shen, Jiong Wu, Zhimin Shao, Guangyu Liu
OBJECTIVE: To investigate ovarian function and therapeutic efficacy among estrogen receptor (ER)-positive, premenopausal breast cancer patients treated with gonadotropin-releasing hormone agonist (GnRHa) and chemotherapy simultaneously or sequentially. METHOD: This study was a phase 3, open-label, parallel, randomized controlled trial (NCT01712893). Two hundred sixteen premenopausal patients (under 45 years) diagnosed with invasive ER-positive breast cancer were enrolled from July 2009 to May 2013 and randomized at a 1:1 ratio to receive (neo)adjuvant chemotherapy combined with sequential or simultaneous GnRHa treatment...
January 13, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29331901/an-ultrasensitive-detection-of-mirna-155-in-breast-cancer-via-direct-hybridization-assay-using-two-dimensional-molybdenum-disulfide-field-effect-transistor-biosensor
#10
Samira Mansouri Majd, Abdollah Salimi, Foad Ghasemi
MicroRNAs (miRNAs), critical biomarkers of acute and chronic diseases, play key regulatory roles in many biological processes. As a result, robust assay platforms to enable an accurate and efficient detection of low-level miRNAs in complex biological samples are of great significance. In this work, a label-free and direct hybridization assay using molybdenum disulfide (MoS2) field-effect transistor (FET) biosensor has been developed for ultrasensitive detection of miRNA-155 as a breast cancer biomarker in human serum and cell-line samples...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331612/arterial-spin-labeling-cerebral-perfusion-magnetic-resonance-imaging-in-migraine-aura-an-observational-study
#11
Marc E Wolf, Shuhei Okazaki, Philipp Eisele, Christina Rossmanith, Johannes Gregori, Martin Griebe, Matthias Günther, Achim Gass, Michael G Hennerici, Kristina Szabo, Rolf Kern
BACKGROUND: Changes in cerebral perfusion during migraine with aura (MA) have been assessed mainly using dynamic susceptibility contrast (DSC) magnetic resonance perfusion imaging. A contrast agent-free method to assess these changes would be desirable. We assessed changes in cerebral perfusion during MA using arterial spin labeling (ASL) perfusion magnetic resonance imaging. METHODS: We investigated 4 patients with a standardized protocol including ASL perfusion imaging during MA (n = 2) or early headache phase (n = 2) and asymptomatic follow-up...
January 10, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/29331425/low-picomolar-label-free-procalcitonin-analytical-detection-with-an-electrolyte-gated-organic-field-effect-transistor-based-electronic-immunosensor
#12
Preethi Seshadri, Kyriaki Manoli, Nicole Schneiderhan-Marra, Uwe Anthes, Piotr Wierzchowiec, Klaus Bonrad, Cinzia Di Franco, Luisa Torsi
Herein a label-free immunosensor based on electrolyte-gated organic field-effect transistor (EGOFET) was developed for the detection of procalcitonin (PCT), a sepsis marker. Antibodies specific to PCT were immobilized on the poly-3-hexylthiophene (P3HT) organic semiconductor surface through direct physical adsorption followed by a post-treatment with bovine serum albumin (BSA) which served as the blocking agent to prevent non-specific adsorption. Antibodies together with BSA (forming the whole biorecognition layer) served to selectively capture the procalcitonin target analyte...
December 26, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331424/ultra-narrow-surface-lattice-resonances-in-plasmonic-metamaterial-arrays-for-biosensing-applications
#13
Artem Danilov, Gleb Tselikov, Fan Wu, Vasyl G Kravets, Igor Ozerov, Frederic Bedu, Alexander N Grigorenko, Andrei V Kabashin
When excited over a periodic metamaterial lattice of gold nanoparticles (~ 100nm), localized plasmon resonances (LPR) can be coupled by a diffraction wave propagating along the array plane, which leads to a drastic narrowing of plasmon resonance lineshapes (down to a few nm full-width-at-half-maximum) and the generation of singularities of phase of reflected light. These phenomena look very promising for the improvement of performance of plasmonic biosensors, but conditions of implementation of such diffractively coupled plasmonic resonances, also referred to as plasmonic surface lattice resonances (PSLR), are not always compatible with biosensing arrangement implying the placement of the nanoparticles between a glass substrate and a sample medium (air, water)...
December 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331073/cerebrospinal-fluid-macrophage-biomarkers-in-amyotrophic-lateral-sclerosis
#14
A G Thompson, E Gray, M-L Thézénas, P D Charles, S Evetts, M T Hu, K Talbot, R Fischer, B M Kessler, M R Turner
Objective The neurodegenerative disease amyotrophic lateral sclerosis (ALS) is a heterogeneous clinical syndrome involving multiple molecular pathways. The development of biomarkers for use in therapeutic trials is a priority. We sought to use a high-throughput proteomic method to identify novel biomarkers in individual cerebrospinal fluid samples. Methods Liquid chromatography-tandem mass spectrometry with label-free quantification was used to identify cerebrospinal fluid proteins using samples from a well-characterised longitudinal cohort comprising patients with ALS (n=43), the upper motor neuron variant primary lateral sclerosis (PLS, n=6), cross-sectional healthy (n=20) and disease controls (Parkinsons's n=20, ALS mimic disorders n=12)...
January 13, 2018: Annals of Neurology
https://www.readbyqxmd.com/read/29330787/mass-spectrometry-and-bioinformatic-analysis-of-hydroxylation-dependent-protein-protein-interactions
#15
Javier Rodriguez, Alex von Kriegsheim
Characterization of how a stimulus regulates the dynamics of protein-protein interaction is critical for understanding how a particular protein is regulated in an intracellular signaling network. Protein hydroxylation, which is a posttranslational modification catalyzed by oxygen-dependent enzymes, is a crucial regulator of protein-protein interactions. Under low oxygen conditions, the activity of many hydroxylases is inhibited, which results in a reduction of substrate hydroxylation. These changes alter the interactome of the substrate, and this dynamic rewiring of signaling networks explains crucial aspects of the adaptive response to hypoxia...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330719/prognostic-value-of-tumor-infiltrating-lymphocyte-density-assessed-using-a-standardized-method-based-on-molecular-subtypes-and-adjuvant-chemotherapy-in-invasive-breast-cancer
#16
Nuri Jang, Hee Jung Kwon, Min Hui Park, Su Hwan Kang, Young Kyung Bae
BACKGROUND: This study investigated the prognostic value of tumor-infiltrating lymphocyte (TIL) density as determined by molecular subtype and receipt of adjuvant chemotherapy in invasive breast cancer (IBC). METHODS: Stromal TIL densities were evaluated in 1489 IBC samples using recommendations proposed by the International TILs Working Group. Cases were allocated to high- and low-TIL density groups using a cutoff of 10%. RESULTS: Of the 1489 IBC patients, 427 (28...
January 12, 2018: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/29330374/predicting-fibrosis-progression-in-renal-transplant-recipients-using-laser-based-infrared-spectroscopic-imaging
#17
Vishal K Varma, Andre Kajdacsy-Balla, Sanjeev Akkina, Suman Setty, Michael J Walsh
Renal transplants have not seen a significant improvement in their 10-year graft life. Chronic damage accumulation often leads to interstitial fibrosis and tubular atrophy (IF/TA) and thus graft function loss over time. For this reason, IF/TA has been the chief suspect for a potential prognostic marker for long term outcomes. In this study, we have used infrared spectroscopic (IR) imaging to interrogate the biochemistry of regions of fibrosis from renal transplant biopsies to identify a biochemical signature that can predict rapid progression of fibrosis...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330032/a-free-sugars-daily-value-dv-identifies-more-less-healthy-prepackaged-foods-and-beverages-than-a-total-sugars-dv
#18
Jodi T Bernstein, Marie-Ève Labonté, Beatriz Franco-Arellano, Alyssa Schermel, Mary R L'Abbé
Regulatory changes in Canada will require food labels to have a benchmark [% Daily Value, %DV] for total sugars, based on 100 g/day, while US labels will require a %DV for added sugars, based on 50 g/day. The objective of this study was to compare two labelling policies, a total sugars DV (100 g/day) and a free sugars DV (50 g/day) on food labels. This cross-sectional analysis of the Food Label Information Program database focussed on top sources of total sugars intake in Canada (n = 6924 foods)...
January 9, 2018: Preventive Medicine
https://www.readbyqxmd.com/read/29329773/total-energy-expenditure-and-body-composition-of-children-with-developmental-disabilities
#19
Michele Polfuss, Kathleen J Sawin, Paula E Papanek, Linda Bandini, Bethany Forseth, Andrea Moosreiner, Kimberley Zvara, Dale A Schoeller
BACKGROUND: Obesity prevalence is increased in children with developmental disabilities, specifically in children with spina bifida and Down syndrome. Energy expenditure, a critical aspect of weight management, has been extensively studied in the typically developing population, but not adequately studied in children with developmental disabilities. OBJECTIVE: Determine energy expenditure, fat-free mass and body fat percentile and the impact of these findings on recommended caloric intake in children with spina bifida and Down syndrome...
December 28, 2017: Disability and Health Journal
https://www.readbyqxmd.com/read/29329083/a-label-free-electrochemical-system-for-comprehensive-monitoring-of-o-chlorophenol
#20
Xiaolin Zhu, Kexin Zhang, Nan Lu, Yangyang Yu, Xing Yuan, Jiunian Guan
o-Chlorophenol (OCP) is a priority pollutant that poses serious health threats to the public. The following study designs a simple electrochemical system to monitor the concentration and toxicity of OCP. This system was primarily characterized by the integration of both physicochemical and biological monitoring procedures that had a synergistic effect between the functionalized carbon nanotubes and rhodamine B. This resulted in excellent electrocatalytic activities toward OCP and cellular purine bases. The peak current of OCP was linear with concentrations ranging from 0...
January 4, 2018: Chemosphere
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