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https://www.readbyqxmd.com/read/28918421/macrophage-activation-syndrome-at-the-onset-of-glucocorticoid-resistant-systemic-lupus-erythematosus-a-case-report
#1
Delia Tulbă, Marius Balea, Cristian Băicuș
INTRODUCTION: Macrophage activation syndrome (MAS) is a life-threatening hyperinflammatory state mediated by uncontrolled cytokine storm and haemophagocytosis. Although rarely reported, MAS might occur in systemic lupus erythematosus (SLE), notably as an inaugural manifestation. Glucocorticoids (GCs) are the cornerstone of SLE therapy. However, in some cases high doses of GCs are required to achieve remission (i.e. glucocorticoid-resistance), leading to significant side effects. CASE REPORT: A 28-year-old Romani male was admitted to our hospital for polyarthralgia, polyserositis and fatigability...
September 16, 2017: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/28918279/seasonal-variation-of-chloro-s-triazines-in-the-hartbeespoort-dam-catchment-south-africa
#2
Cornelius Rimayi, David Odusanya, Jana M Weiss, Jacob de Boer, Luke Chimuka
Seasonal variation of eight chloro-s-triazine herbicides and seven major atrazine and terbuthylazine degradation products was monitored in the Hartbeespoort Dam catchment using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS). Lake, river and groundwater were sampled from the Hartbeespoort Dam catchment over four seasons and the downstream Jukskei River was monitored during the winter season. Triazine herbicide concentrations in the Hartbeespoort Dam were in the order atrazine>simazine>propazine>ametryn>prometryn throughout the four seasons sampled...
September 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28918162/how-high-level-of-anxiety-in-panic-disorder-can-interfere-in-working-memory-a-computer-simulation-and-electrophysiological-investigation
#3
Luiza Wanick Di Giorgio Silva, Danielle Aprigio, Jesse Di Giacomo, Mariana Gongora, Henning Budde, Juliana Bittencourt, Mauricio Cagy, Silmar Teixeira, Pedro Ribeiro, Marcele Regine de Carvalho, Rafael Freire, Antonio Egidio Nardi, Luis Fernando Basile, Bruna Velasques
Panic disorder (PD) is characterized by repeated and unexpected attacks of intense anxiety, which are not restricted to a determined situation or circumstance. The coherence function has been used to investigate the communication among brain structures through the quantitative EEG (qEEG). The objective of this study is to analyze if there is a difference in frontoparietal gamma coherence (GC) between panic disorder patients (PDP) and healthy controls (HC) during the Visual oddball paradigm; and verify if high levels of anxiety (produced by a computer simulation) affect PDP's working memory...
September 11, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28918030/lncrna-trerna1-function-as-an-enhancer-of-snai1-promotes-gastric-cancer-metastasis-by-regulating-epithelial-mesenchymal-transition
#4
Huazhang Wu, Ying Hu, Xiufang Liu, Wei Song, Pihai Gong, Kun Zhang, Zhenxing Chen, Menghan Zhou, Xiaohui Shen, Yanyan Qian, Hong Fan
Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis of gastric cancer (GC). TRERNA1 is significantly unregulated in GCs and GC cell lines. Increased TRERNA1 is positively correlated with lymph node metastasis of GCs. RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays revealed that TRERNA1 functions as a scaffold to recruit EZH2 to epigenetically silence epithelial-mesenchymal transition marker CDH1 by H3K27me3 of its promoter region...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917968/the-effect-of-fennel-essential-oil-in-combination-with-antibiotics-on-staphylococcus-aureus-strains-isolated-from-carriers
#5
Paweł Kwiatkowski, Magdalena Mnichowska-Polanowska, Agata Pruss, Helena Masiuk, Małgorzata Dzięcioł, Stefania Giedrys-Kalemba, Monika Sienkiewicz
An increase in the number of staphylococcal infections and carriers among medical staff has forced us to seek more and more effective antibacterial agents. Bacteria from the Staphylococcus genus possessing different mechanisms of resistance are the cause of nosocomial infections. The objective of our investigations was susceptibility of S. aureus strains isolated from nasal vestibule of medical students to fennel essential oil. The GC-MS analysis of fennel essential oil revealed eleven constituents among which a majority of trans-anethole (80%) was found...
September 13, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28917930/structural-characterization-and-rheological-behavior-of-a-heteroxylan-extracted-from-plantago-notata-lagasca-plantaginaceae-seeds
#6
Fatima Benaoun, Cédric Delattre, Zakaria Boual, Alina V Ursu, Christophe Vial, Christine Gardarin, Anne Wadouachi, Didier Le Cerf, Tony Varacavoudin, Mohamed Didi Ould El-Hadj, Philippe Michaud, Guillaume Pierre
Plantago notata (Plantaginaceae) is a spontaneous plant from Septentrional Algerian Sahara currently used by traditional healers to treat stomach disorders, inflammations or wound healing. A water-soluble polysaccharide, called PSPN (PolySaccharide fraction from Plantago Notata), was extracted and purified from the seeds of this semi-arid plant. The structural features of this mucilage were evaluated by colorimetric assays, Fourier transformed infrared spectroscopy (FT-IR), gas chromatography coupled to mass spectrometry (GC/MS) and (1)H/(13)C Nuclear Magnetic Resonance (NMR) spectroscopy...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917908/structural-elucidation-of-the-main-water-soluble-polysaccharide-from-rubus-anatolicus-roots
#7
Neda Sahragard, Kambiz Jahanbin
An acidic heteropolysaccharide, RAPS-1, was isolated from the roots of Rubus anatolicus by water extraction (70°C) and purification using DEAE-cellulose A52 and Sephadex G-100 column chromatography. The total sugar content and specific optical rotation of RAPS-1 were 96.3% and +196°, respectively. RAPS-1 had a molecular weight of 7900Da, and was composed of glucose, galactose, and glucuronic acid with a relative molar ratio of 6.2: 1.0: 1.2. Structural features of RAPS-1 were elucidated by a combination of partial acid hydrolysis, periodate oxidation and Smith degradation, methylation, GC-MS, FTIR, and NMR ((13)C and (1)H) analysis...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917881/structural-characterization-and-rheological-properties-of-a-galactomannan-from-astragalus-gombo-bunge-seeds-harvested-in-algerian-sahara
#8
T Chouana, G Pierre, C Vial, C Gardarin, A Wadouachi, D Cailleu, D Le Cerf, Z Boual, M D Ould El Hadj, P Michaud, C Delattre
A water soluble polysaccharide (WSP) was extracted and purified from Astragalus gombo seeds (Fabaceae) harvested in Septentrional Sahara (Ouargla, Algeria) with a yield of 6.8% (w/w of the dry seed ground). It was characterized by gas chromatography coupled to the mass spectrometry (GC-MS), size exclusion chromatography with Multi-Angle Light Scattering analysis (SEC-MALLS), high-resolution (1)H and (13)C NMR, and rheological measurements. The structural characterization indicated that this WSP fraction is a galactomannan with a mannose/galactose ratio of 1...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917841/graphene-vp40-interactions-and-potential-disruption-of-the-ebola-virus-matrix-filaments
#9
Jeevan B Gc, Rudramani Pokhrel, Nisha Bhattarai, Kristen A Johnson, Bernard S Gerstman, Robert V Stahelin, Prem P Chapagain
Ebola virus infections cause hemorrhagic fever that often results in very high fatality rates. In addition to exploring vaccines, development of drugs is also essential for treating the disease and preventing the spread of the infection. The Ebola virus matrix protein VP40 exists in various conformational and oligomeric forms and is a potential pharmacological target for disrupting the virus life-cycle. Here we explored graphene-VP40 interactions using molecular dynamics simulations and graphene pelleting assays...
September 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28917789/chemometric-optimization-of-the-extraction-and-derivatization-of-parabens-for-their-determination-in-water-samples-by-rotating-disk-sorptive-extraction-and-gas-chromatography-mass-spectrometry
#10
Mercedes Becerra-Herrera, Valentina Miranda, Daniel Arismendi, Pablo Richter
A combination of rotating disk sorptive extraction (RDSE) using Oasis® HLB as the sorbent phase and gas chromatography mass spectrometry (GC-MS) has been performed for the determination of four of the most widely used parabens: methylparaben, ethylparaben, propylparaben and n-butylparaben. The extraction and derivatization of the analytes in water samples were optimized by using factorial (screening) and Doehlert designs, thus reducing the number of analyses with the concomitant reduction of time, reagents, waste, samples and cost...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917787/gas-chromatographic-mass-spectrometric-analysis-of-urinary-volatile-organic-metabolites-optimization-of-the-hs-spme-procedure-and-sample-storage-conditions
#11
Tanja Živković Semren, Irena Brčić Karačonji, Toni Safner, Nataša Brajenović, Blanka Tariba Lovaković, Alica Pizent
Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify potential biomarkers associated with the changes in metabolism related to various health disorders. To ensure reliable analysis of urinary volatile organic metabolites (VOMs) by gas chromatography-mass spectrometry (GC-MS), parameters affecting the headspace-solid phase microextraction (HS-SPME) procedure have been evaluated and optimized. The influence of incubation and extraction temperatures and times, coating fibre material and salt addition on SPME efficiency was investigated by multivariate optimization methods using reduced factorial and Doehlert matrix designs...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917783/intellectual-modifying-a-bare-glassy-carbon-electrode-to-fabricate-a-novel-and-ultrasensitive-electrochemical-biosensor-application-to-determination-of-acrylamide-in-food-samples
#12
Kambiz Varmira, Omid Abdi, Mohammad-Bagher Gholivand, Hector C Goicoechea, Ali R Jalalvand
Acrylamide (AA) is a neurotoxin and carcinogen which is mainly formed in foods containing large quantities of starch processed at high temperatures and its determination is very important to control the quality of foods. In this work, a novel electrochemical biosensor based on hemoglobin-dimethyldioctadecylammonium bromide (HG-DDAB)/platinum-gold-palladium three metallic alloy nanoparticles (PtAuPd NPs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (Ch-IL)/multiwalled carbon nanotubes-IL (MWCNTs-IL)/glassy carbon electrode (GCE) is proposed for ultrasensitive determination of AA in food samples...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917778/determination-of-volatile-organic-compounds-in-eucalyptus-fast-pyrolysis-bio-oil-by-full-evaporation-headspace-gas-chromatography
#13
Nathalya Kosinski Lima, André Romualdo Lopes, Palimecio Gimenes Guerrero, Carlos Itsuo Yamamoto, Fabricio Augusto Hansel
This paper reports a full evaporation (FE) headspace gas chromatographic (HS-GC) method for the determination of the volatile organic compounds (VOCs) in bio-oil (i.e. methanol, ethanol, acetone, acetic acid and furfural). The method uses a 4μL sample of bio-oil in a headspace vial (ca. 20mL). Complete evaporation of the compounds was achieved after seven minutes at 90°C. The method showed good precision and accuracy for methanol, ethanol, acetone and acetic acid. The recovery of furfural was low (74.3%)...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917775/fabrication-of-bi-monomer-copolymer-of-pyrrole-indole-for-highly-efficient-solid-phase-microextraction-of-benzene-derivatives
#14
Jie Zhang, Baizhao Zeng, Faqiong Zhao
A procedure for direct electrochemical deposition of poly(pyrrole-indole) on gold nanoparticles coated stainless steel wire was established, and the formation of copolymer was confirmed by infrared spectroscopy. The synthesized coating showed unique microstructure, excellent extraction efficiency (2-10 times of corresponding single-component coating), high thermal stability (up to 280°C) and good durability (could be used for more than 200 times). As a novel and promising extraction coating, it was used for the headspace solid phase microextraction-gas chromatography detection of some benzene derivatives, including chlorobenzene, bromobenzene, p-bromotoluene, m-nitrotoluene and p-nitrotoluene...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917754/quantification-of-7-aminoflunitrazepam-in-human-urine-by-polymeric-monolith-based-capillary-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#15
Yu-Ru Wu, Hsiang-Yu Liu, Shu-Ling Lin, Ming-Ren Fuh
Using a simple liquid-liquid extraction (LLE) procedure for sample pretreatment, 7-Aminoflunitrazepam (7-aminoFM2), a major metabolite of flunitrazepam (FM2), was determined in urine samples by polymeric monolith-based capillary liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The linearity was found in the range of 0.1-50ngmL(-1) with a method detection limit (signal-to-noise ratio of 3) estimated at 0.05ngmL(-1). Using the proposed method, good precision and recovery were also found in spiked urine samples at the levels of 0...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917749/trace-determination-of-volatile-polycyclic-aromatic-hydrocarbons-in-natural-waters-by-magnetic-ionic-liquid-based-stir-bar-dispersive-liquid-microextraction
#16
Juan L Benedé, Jared L Anderson, Alberto Chisvert
In this work, a novel hybrid approach called stir bar dispersive liquid microextraction (SBDLME) that combines the advantages of stir bar sorptive extraction (SBSE) and dispersive liquid-liquid microextraction (DLLME) has been employed for the accurate and sensitive determination of ten polycyclic aromatic hydrocarbons (PAHs) in natural water samples. The extraction is carried out using a neodymium stir bar magnetically coated with a magnetic ionic liquid (MIL) as extraction device, in such a way that the MIL is dispersed into the solution at high stirring rates...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917736/off-line-microextraction-by-packed-sorbent-combined-with-on-solid-support-derivatization-and-gc-ms-application-for-the-analysis-of-five-pyrethroid-metabolites-in-urine-samples
#17
Anna Klimowska, Bartosz Wielgomas
A novel, fast and eco-friendly analytical method using microextraction by packed sorbent coupled to large volume injection-gas chromatography-mass spectrometry (MEPS-LVI-GC-MS) was developed for the determination of five pyrethroid metabolites (cis-2,2-dimethyl-3-(2-chloro-3,3,3-trifluoro-1-propenyl)-cyclopropanecarboxylic acid, cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acids, cis-(2,2-dibromovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid and 3-phenoxybenzoic acid) in human urine...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917728/fast-method-for-the-simultaneous-determination-of-monomethylmercury-and-inorganic-mercury-in-rice-and-aquatic-plants
#18
María Jiménez-Moreno, Miguel Ángel Lominchar, María José Sierra, Rocío Millán, Rosa Carmen Rodríguez Martín-Doimeadios
Recent investigations revealed that monomethylmercury (MMHg) can be absorbed and accumulated by plants, i.e. rice crops, thus becoming an important route of human exposure to MMHg through diet. The increasing concern about this fact makes that appropriate analytical methods for Hg speciation in these samples are urgently required. Therefore, the aim of this work has been the development of a fast and sensitive method which enables the simultaneous determination of MMHg and inorganic Hg in rice and aquatic plants...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917212/comparative-analytical-and-toxicological-assessment-of-methylcyclohexanemethanol-mchm-mixtures-associated-with-the-elk-river-chemical-spill
#19
Katharine A Horzmann, Chloe de Perre, Linda S Lee, Andrew J Whelton, Jennifer L Freeman
On January 9, 2014, a chemical mixture containing crude methylcyclohexanemethanol (MCHM) contaminated the water supply of Charleston, West Virginia. Although the mixture was later identified as a mix of crude MCHM and stripped propylene glycol phenyl ethers, initial risk assessment focused on 4-MCHM, the predominant component of crude MCHM. The mixture's exact composition and the toxicity differences between 4-MCHM, crude MCHM, and the tank mixture were unknown. We analyzed the chemical composition of crude MCHM and the tank mixture via GC/MS and, based on identified spectra, found that crude MCHM and the tank mixture differed in chemical composition...
September 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28916460/the-effect-of-viroid-infection-of-citrus-trees-on-the-amoebicidal-activity-of-maltese-half-blood-citrus-sinensis-against-trophozoite-stage-of-acanthamoeba-castellanii-neff
#20
Ghaya Zouaghi, Asma Najar, Olfa Chiboub, Ines Sifaoui, Manef Abderrabba, Jacob Lorenzo Morales
In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three samples of peels' essential oils were studied: from a healthy plant (Control), a plant inoculated with Citrus exocortis viroid (CEVd) and one inoculated with hot stunt cachexia viroid (HSVd). The samples were extracted by hydrodistillation from dried peels and characterized by GC-MS...
September 12, 2017: Experimental Parasitology
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