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https://www.readbyqxmd.com/read/28102871/correction-elucidating-time-dependent-changes-in-the-urinary-metabolome-of-renal-transplant-patients-by-a-combined-1-h-nmr-and-gc-ms-approach
#1
Kienana Muhrez, Lydie Nadal-Desbarats, Jean-Michel Halimi, Binta Dieme, Matthias Büchler, Patrick Emond, Hélène Blasco, Chantal Le Guellec
Correction for 'Elucidating time-dependent changes in the urinary metabolome of renal transplant patients by a combined (1)H NMR and GC-MS approach' by Kienana Muhrez et al., Mol. BioSyst., 2015, 11, 2493-2510.
January 19, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28102075/molecular-features-of-humic-acids-and-fulvic-acids-from-contrasting-environments
#2
Judith Schellekens, Peter Buurman, Karsten Kalbitz, Andre van Zomeren, Pablo Vidal-Torrado, Chiara Cerli, Rob N J Comans
Insight in the molecular structure of humic acid (HA) and fulvic acid (FA) can contribute to identify relationships between their molecular properties, and further our quantitative abilities to model important organic matter functions such as metal complexation and association with mineral surfaces. Pyrolysis gas chromatography/mass spectrometry (Py-GC-MS) is used to compare the molecular composition of HA and FA. A systematic comparison was obtained by using samples from different environmental sources, including solid and aqueous samples from both natural and waste sources...
January 19, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28101726/development-of-timolol-loaded-galactosylated-chitosan-nanoparticles-and-evaluation-of-their-potential-for-ocular-drug-delivery
#3
Ruobing Zhao, Jing Li, Jingyi Wang, Zhenya Yin, Yongtao Zhu, Wei Liu
This study was conducted to develop timolol maleate (TM)-loaded galactosylated chitosan (GC) nanoparticles (NPs) (TM-GC-NPs) followed by optimization via a four-level and three-factor Box-Behnken statistical experimental design. The optimized nanoparticles showed a particle size of 213.3 ± 6.83 nm with entrapment efficiency of 38.58 ± 1.31% and drug loading of 17.72 ± 0.28%. The NPs were characterized with respect to zeta potential, pH, surface morphology, and differential scanning calorimetry (DSC)...
January 18, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28101114/dietary-and-socio-demographic-determinants-of-serum-persistent-organic-pollutants-pops-levels-in-pregnant-women-in-tehran
#4
Bita Eslami, Batool Hossein-Rashidi, Kazem Naddafi, Noushin Rastkari, Abolghasem Djazayeri, Hossein Malekafzali
Objective: To determine the levels of Polychlorinated biphenyls (PCBs) and Polybrominateddiphenyl ethers (PBDEs) as persistent organic pollutants (POPs) in serum of primiparous women at the third trimester of pregnancy and identify the main determinants of POPs levels such as socio-demographic, lifestyle, and diet in Tehran. Materials and methods: One- hundred eighty five serum samples from two simultaneous case-control studies were collected from September 2013 until August 2015.Ten most abundant PCB congeners (International Union of Pure and Applied Chemistry (IUPAC) congeners 28, 52, 74, 99, 101, 118, 138, 153, 180 and 187) as well as eight PBDE congeners (IUPAC congeners 28, 47, 99, 100, 153, 154, 183 and 209)were analyzed by Gas Chromatography Mass Spectrometry (GC/MS)...
September 2016: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/28100914/tet-mediated-hydroxymethylcytosine-at-the-ppar%C3%AE-locus-is-required-for-initiation-of-adipogenic-differentiation
#5
Y Yoo, J H Park, C Weigel, D B Liesenfeld, D Weichenhan, C Plass, D-G Seo, A M Lindroth, Y J Park
BACKGROUND/OBJECTIVES: Adipose tissue is one of the main organs regulating energy homeostasis, via energy storage as well as endocrine function. The adipocyte cell number is largely determined by adipogenesis. While the molecular mechanism of adipogenesis has been extensively studied, its role in dynamic DNA methylation plasticity remains unclear. Recently, it has been shown that Tet methylcytosine dioxygenase (TET) are catalytically capable of oxidizing DNA 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) towards a complete removal of the methylated cytosine...
January 19, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28100886/characterization-of-volatile-compounds-with-hs-spme-from-oxidized-n-3-pufa-rich-oils-via-rancimat-tests
#6
Kai-Min Yang, Ming-Ching Cheng, Chih-Wei Chen, Chin-Yin Tseng, Li-Yun Lin, Po-Yuan Chiang
Algae oil and fish oil are n-3 PUFA mainstream commercial products. The various sources for the stability of n-3 PUFA oxidation are influenced by the fatty acid composition, extraction and refined processing. In this study, the oil stability index (OSI) occurs within 2.3 to 7.6 hours with three different n-3 PUFA rich oil. To set the OSI in the Rancimat test as the oil stability limit and observed various degrees of oxidation (0, 25, 50, 75, 100 and 125%). The volatile oxidation compounds were analyzed via headspace-solid phase microextraction (HS-SPME) and GC/MS...
January 18, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28100885/quantitative-analysis-of-the-distribution-of-cis-eicosenoic-acid-positional-isomers-in-marine-fishes-from-the-indian-ocean
#7
Samanthika Senarath, Kazuaki Yoshinaga, Toshiharu Nagai, Akihiko Yoshida, Fumiaki Beppu, Chamila Jayasinghe, Chandravathany Devadawson, Naohiro Gotoh
This study investigated the occurrence and distribution of cis-eicosenoic acid (c-20:1) positional isomers in fishes from the Indian Ocean and compared to those from the Pacific and Atlantic Ocean. Lipids were extracted from the edible part of the fish and then methylated. The eicosenoic acid methyl ester fraction was separated from total fatty acid methyl esters by reversed-phase HPLC and quantitatively analyzed using a GC-FID fitted with the SLB-IL111 highly polar GC column. c14-20:1 was used as an internal standard...
January 18, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28100741/control-of-spike-transfer-at-hippocampal-mossy-fiber-synapses-in-vivo-by-gabaa-and-gabab-receptor-mediated-inhibition
#8
Stefano Zucca, Marilena Griguoli, Meryl Malézieux, Noëlle Grosjean, Mario Carta, Christophe Mulle
: Despite extensive studies in hippocampal slices and incentive from computational theories, the synaptic mechanisms underlying information transfer at mossy fiber (mf) connections between the dentate gyrus (DG) and CA3 neurons in vivo are still elusive. Here we used an optogenetic approach in mice to selectively target and control the activity of DG granule cells (GCs) while performing whole-cell and juxtacellular recordings of CA3 neurons in vivo In CA3 pyramidal cells (PCs), mf-CA3 synaptic responses consisted predominantly of an IPSP at low stimulation frequency (0...
January 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28100620/overexpression-of-il-7-extends-the-humoral-immune-response-induced-by-rabies-vaccination
#9
Yingying Li, Ming Zhou, Zhaochen Luo, Yachun Zhang, Min Cui, Huanchun Chen, Zhen F Fu, Ling Zhao
: Rabies continues to present a public health threat in most countries of the world. The most efficient way to prevent and control rabies is to implement vaccination programmes for domestic animals. However, traditional inactivated vaccines used in animals are costly and have a relatively low efficiency, which impedes their extensive use in developing countries. There is therefore an urgent need to develop single-dose and long-lasting rabies vaccines. However, little information is available regarding the mechanisms underlying immunological memory, which can broaden humoral responses following rabies vaccination...
January 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28100567/mecp2-regulated-glycogenes-contribute-to-proliferation-and-apoptosis-of-gastric-cancer-cells
#10
Yannan Qin, Lingyu Zhao, Xiaofei Wang, Dongdong Tong, Christopher Hoover, Fei Wu, Yingxun Liu, Lumin Wang, Liying Liu, Lei Ni, Tusheng Song, Chen Huang
Aberrant glycogene and glycan expression is intimately associated with carcinogenesis, invasion, and metastasis of gastric cancer (GC); however the regulatory mechanisms for glycogenes in GC cells remain unclear. Methyl-CpG-binding protein 2 (MeCP2) regulates genes by binding to methylated promoters, and in our previous work we found that it is overexpressed in GC cell lines and tissues, functioning as an oncogene. In this study we detected the expression of 212 glycogenes in MeCP2 silenced GC cells versus control using the Agilent Whole Human Genome Microarray and mining the data through bioinformatic analysis...
January 18, 2017: Glycobiology
https://www.readbyqxmd.com/read/28100224/radical-scavenging-activities-of-lagerstroemia-speciosa-l-pers-petal-extracts-and-its-hepato-protection-in-ccl4-intoxicated-mice
#11
Bipransh Kumar Tiwary, Somit Dutta, Priyankar Dey, Mossaraf Hossain, Anoop Kumar, Sony Bihani, Ashis Kumar Nanda, Tapas Kumar Chaudhuri, Ranadhir Chakraborty
BACKGROUND: Lagerstroemia speciosa (L.) Pers. has medicinal importance. Bioactive phytochemicals isolated from different parts of L. speciosa, have revealed hypoglycemic, antibacterial, anti-inflammatory, antioxidant and hepato protective properties. Despite one report from Philippines detailing the use of L. speciosa as curative for fever and as well as diuretic, there is no experimental evidence about the hepatoprotective activity of the flower extracts. METHODS: Several spectroscopic methods, including GC-MS, were used to characterize phytochemicals present in the petal extract of L...
January 18, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28100121/effects-of-bovine-pregnancy-on-the-fatty-acid-composition-of-milk-significance-for-humans-needs
#12
R Barreiro, P Regal, M Díaz-Bao, B I Vázquez, A Cepeda
Milk from forty Holstein dairy cows (twenty pregnant and twenty non-pregnant) was collected from two different farms in Galicia (Spain). The differences in the fatty acid composition of two groups of cows (pregnant and non-pregnant) was studied to determine whether pregnancy status is a determinant factor that can alter the fatty acid profile of milk. Gas-chromatography (GC) coupled to flame ionization detection (FID) was used for determination of the fatty acids. Difference in the milk fatty acids between pregnant and non-pregnant cows were pronounced showing statistically significant differences for some fatty acids and the total saturated and monounsaturated fatty acids...
January 19, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28100049/cell-free-translation-is-more-variable-than-transcription
#13
Fabio Chizzolini, Michele Forlin, Noël Yeh Martín, Giuliano Berloffa, Dario Cecchi, Sheref S Mansy
Although RNA synthesis can be reliably controlled with different T7 transcriptional promoters during cell-free gene expression with the PURE system, protein synthesis remains largely unaffected. To better control protein levels, a series of ribosome binding sites (RBS) was investigated. While RBS strength did strongly affect protein synthesis, the RBS sequence could explain less than half of the variability of the data. Protein expression was found to depend on other factors besides the strength of the RBS, including GC content...
January 19, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28099582/a-simple-and-efficient-method-for-poly-3-hydroxybutyrate-quantification-in-diazotrophic-bacteria-within-5-minutes-using-flow-cytometry
#14
L P S Alves, A T Almeida, L M Cruz, F O Pedrosa, E M de Souza, L S Chubatsu, M Müller-Santos, G Valdameri
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry...
January 16, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28099578/il-6-and-il-10-gene-polymorphisms-in-patients-with-aggressive-periodontitis-effects-on-gcf-serum-and-clinic-parameters
#15
Emine Pirim Gorgun, Hulya Toker, Ertan Mahir Korkmaz, Omer Poyraz
Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study...
January 16, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28099062/rectal-carriage-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-gram-negative-bacilli-in-intensive-care-units-in-tunisia
#16
Samia Hammami, Chaima Dahdeh, Kelthoum Mamlouk, Sana Ferjeni, Elaa Maamar, Zeineb Hamzaoui, Mabrouka Saidani, Salma Ghedira, Mohamed Houissa, Amin Slim, Ilhem Boutiba-Ben Boubaker
This study was conducted to evaluate the rate of fecal carriage of Gram-negative bacilli (GNB) resistant to third-generation cephalosporins (third GC) in patients hospitalized in the intensive care unit (ICU) of Charles Nicolle Hospital of Tunis and to identify the enzymatic mechanisms involved. From February to April 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. They were cultured on desoxycholate-lactose-agar plates supplemented with cefotaxime (2 mg/L)...
January 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28098995/a-chemometrics-approach-to-the-investigation-of-the-intraspecific-variability-of-the-volatile-oil-of-eupatorium-tremulum-from-southern-brazil
#17
Tiago J T de Souza, Sérgio A L Bordignon, Miriam A Apel
Eupatorium tremulum is a South American shrub reported to cause cattle digestive intoxication, of which the volatile oil, mainly composed by bisabolane- and amorphane-type sesquiterpenoids, exhibits high quantitative variability. This report describes the application of chemometric tools for the identification of volatile compounds that characterize phenophasical changes in the plant. Preblooming, blooming, and postblooming specimens were paired-sampled and submitted to hydrodistillation and GC-MS analysis...
January 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28098914/role-of-mir-647-in-human-gastric-cancer-suppression
#18
Wenlong Cao, Weiyuan Wei, Zexu Zhan, Dongyi Xie, Yubo Xie, Qiang Xiao
MicroRNAs (miRNAs) regulate various oncogenes concomitantly, resulting in tumor suppression. They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC), and was significantly correlated with reduced tumor size and metastasis. In addition, miR-647 was also reduced in GC cell lines. Furthermore, overexpression of miR-647 in the GC cell lines inhibited cell proliferation, promoted cell cycle arrest at the G0/G1 phase and induced cell apoptosis...
January 17, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098891/nhe1-is-upregulated-in-gastric-cancer-and-regulates-gastric-cancer-cell-proliferation-migration-and-invasion
#19
Rui Xie, Haibo Wang, Hai Jin, Guorong Wen, Biguang Tuo, Jingyu Xu
Na+/H+ exchanger isoform 1 (NHE1) is known to play a key role in regulating intracellular pH and osmotic homeostasis and is involved in the development and progression of several types of cancer. However, the function and specific mechanism of NHE1 in gastric cancer (GC) are not clearly understood. In the present study, we report that NHE1 is overexpressed in tissues and cell lines from GC patients, and knockdown or inhibition of NHE1 suppressed GC cell proliferation via regulation of G1/S and G2/M cell cycle phase transitions, concomitant with a marked decrease in positive cell cycle regulators, including cyclin D1 and cyclin B1...
January 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098868/microrna-187-promotes-growth-and-metastasis-of-gastric-cancer-by-inhibiting-foxa2
#20
Cong Li, Sumei Lu, Yubin Shi
MicroRNAs (miRNAs) play an active role in the pathogenesis of gastric cancer. The expression and biological function for miR-187 in gastric cancer remains unknow. In the present study, we demonstrated that miR-187 expression was increased in gastric cancer (GC) tissues and cells. Increased expression level of miR-187 was associated with adverse clinical features including tumor size, lymph metastasis and TNM stage, and decreased overall survival and disease-free survival of GC patients. Functionally, overexpression miR-187 could promote while inhibition of miR-187 could suppress, the proliferation, migration and invasion of GC cells in vitro...
January 16, 2017: Oncology Reports
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