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https://www.readbyqxmd.com/read/28821021/structural-characterization-and-inhibition-on-%C3%AE-glucosidase-activity-of-acidic-polysaccharide-from-annona-squamosa
#1
Yuan-Yuan Ren, Zhen-Yuan Zhu, Hui-Qing Sun, Li-Jing Chen
The crude polysaccharide (TASP3) was extracted from the fruit pulp of Annona squamosa and then isolated and purified by the combination of grading-alcoholic precipitation and Sephadex G-200. The structure of purified polysaccharide (GASP3-3-I) was determined based on the physicochemical and instrumental analyses. The results indicated that GASP3-3-I was an acidic heteropolysaccharide and its average molecular weight was 2.28×10(6)Da. The monosaccharide composition was analysed by GC-MS and ion chromatography, respectively...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820590/conformational-heterogeneity-in-a-fully-complementary-dna-three-way-junction-with-a-gc-rich-branchpoint
#2
Anita Toulmin, Laura E Baltierra-Jasso, Michael J Morten, Tara Sabir, Peter McGlynn, Gunnar F Schröder, Brian O Smith, Steven W Magennis
DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully-complementary 3WJ and found that it contained unpaired bases at the branchpoint, in support of previous observations of branch flexibility and branchpoint reactivity. By combining high-resolution single-molecule FRET, molecular modeling, time-resolved ensemble fluorescence spectroscopy and the first 19F NMR observations of fully complementary 3WJs, we now show that the 3WJ structure can adopt multiple distinct conformations depending upon the sequence at the branchpoint...
August 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28820469/a-third-generation-glucose-biosensor-based-on-cellobiose-dehydrogenase-immobilized-on-a-glassy-carbon-electrode-decorated-with-electrodeposited-gold-nanoparticles-characterization-and-application-in-human-saliva
#3
Paolo Bollella, Lo Gorton, Roland Ludwig, Riccarda Antiochia
Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28820456/deciphering-single-nucleotide-polymorphisms-and-evolutionary-trends-in-isolates-of-the-cydia-pomonella-granulovirus
#4
Jörg T Wennmann, Pit Radtke, Karolin E Eberle, Gianpiero Gueli Alletti, Johannes A Jehle
Six complete genome sequences of Cydia pomonella granulovirus (CpGV) isolates from Mexico (CpGV-M and CpGV-M1), England (CpGV-E2), Iran (CpGV-I07 and CpGV-I12), and Canada (CpGV-S) were aligned and analyzed for genetic diversity and evolutionary processes. The selected CpGV isolates represented recently identified phylogenetic lineages of CpGV, namely, the genome groups A to E. The genomes ranged from 120,816 bp to 124,269 bp. Several common differences between CpGV-M, -E2, -I07, -I12 and -S to CpGV-M1, the first sequenced and published CpGV isolate, were highlighted...
August 18, 2017: Viruses
https://www.readbyqxmd.com/read/28820403/high-levels-of-soluble-vascular-endothelial-growth-factor-receptor-1-sflt1-and-low-levels-of-vascular-endothelial-growth-factor-in-follicular-fluid-on-the-day-of-oocyte-retrieval-correlate-with-ovarian-hyperstimulation-syndrom-regardless-of-the-stimulation
#5
A J Jakimiuk, M A Nowicka, M Zagozda, K Koziol, P Lewandowski, T Issat
The aim of the study was to assess the predictive value of vascular endothelial growth factor (VEGF), its soluble receptor - sVEGF-R1/sFlt1 and endocrine gland-derived vascular endothelial growth factor (EG-VEGF) concentrations in serum and follicular fluid (FF) for ovarian hyperstimulation syndrome (OHSS) in women undergoing controlled ovarian hyperstimulation (COH) protocols. Patients have been divided into 3 groups: control group on natural cycle, patients stimulated with GnRH agonist and patients stimulated with GnRH antagonist...
June 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28820392/orally-administered-angiotensin-converting-enzyme-inhibitors-captopril-and-isoleucine-proline-proline-have-distinct-effects-on-local-renin-angiotensin-system-and-corticosterone-synthesis-in-dextran-sulfate-sodium-induced-colitis-in-mice
#6
H Salmenkari, M Holappa, R A Forsgard, R Korpela, H Vapaatalo
The effects of angiotensin-converting enzyme (ACE) inhibition by an antihypertensive drug, captopril, and milk casein-derived ACE-inhibiting bioactive tripeptide isoleucine-proline-proline (Ile-Pro-Pro), on local renin-angiotensin system (RAS) and glucocorticoid production in the intestine were studied in the dextran sodium sulfate induced colitis in mice. Mice received water or 3% dextran sodium sulfate with or without either 15.7 mg/l captopril or 833 mg/l Ile-Pro-Pro for 7 days. Captopril and Ile-Pro-Pro were found to have distinct effects on local renin-angiotensin system and mRNA expression of glucocorticoid synthesis components in colon in vitro...
June 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28820260/thermally-induced-self-assembly-and-cyclization-of-l-leucyl-l-leucine-in-solid-state
#7
Marat A Ziganshin, Aisylu S Safiullina, Alexander Vladimirovich Gerasimov, Sufia A Ziganshina, Alexander E Klimovitskii, Khasan R Khayarov, Valery V Gorbatchuk
The thermal treatment of oligopeptides is one of the methods for synthesis of organic nanostructures. However, heating may lead not only to self-assembly of the initial molecules, but also to chemical reactions resulting in the formation of new unexpected nanostructures or changing in the properties of the existing ones. In the present work, the reaction of cyclization of dipeptide L-leucyl-L-leucine in solid state under heating was studied. The change in morphology of dipeptide thin film and formation of nanostructures after heating was visualized using atomic force microscopy...
August 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28819827/interaction-between-selp-genetic-polymorphisms-with-inflammatory-cytokine-interleukin-6-il-6-gene-variants-on-cardiovascular-disease-in-chinese-han-population
#8
Lu Kou, Ning Yang, Bo Dong, Yang Li, Jingyu Yang, Qin Qin
The aim of the study is to investigate the impact of SELP and IL-6 genetic single-nucleotide polymorphisms (SNPs) and its gene-gene interaction on cardiovascular disease (CVD) risk based on Chinese population. A total of 1082 subjects (519 males, 563 females), with a mean age of 53.9 ± 13.1 years, were selected, including 540 CVD patients and 542 normal control participants. Logistic regression model was used to examine the association between six SNPs and CVD risk. Odds ratio (OR) and 95% confident interval (95% CI) were calculated...
August 18, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28819779/curcuma-wenyujin-y-h-chen-et-c-ling-n-butyl-alcohol-extract-inhibits-ags-cell-helicobacter-pylori-caga-vaca-promoted-invasiveness-by-down-regulating-caudal-type-homeobox-transcription-factor-and-claudin-2-expression
#9
Hai-Feng Jin, Jin-Feng Dai, Li-Na Meng, Bin Lu
OBJECTIVE: To investigate the effects and possible mechanisms of action of Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl alcohol extract (CWNAE) on repression of human gastric cancer (GC) AGS cell invasion induced by co-culturing with Helicobacter pylori (HP). METHODS: AGS cells were cultured with HP of positive or negative cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) expression (CagA+/- or VacA+/-) and divided into 5 group. Group A was cultured without HP as a control, Group B with HPCagA+VacA+, Group C with HPCagA-VacA-, Group D with HPCagA+VacA+ and CWNAE, and Group E with HPCagA-VacA- and CWNAE...
August 17, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28819581/electrochemical-evaluation-of-trans-resveratrol-levels-in-red-wine-based-on-the-interaction-between-resveratrol-and-graphene
#10
Lantao Liu, Yanli Zhou, Yiyu Kang, Haihong Huang, Congming Li, Maotian Xu, Baoxian Ye
trans-Resveratrol is often considered as one of the quality standards of red wine, and the development of a sensitive and reliable method for monitoring the trans-resveratrol levels in red wine is an urgent requirement for the quality control. Here, a novel voltammetric approach was described for probing trans-resveratrol using a graphene-modified glassy carbon (GC) electrode. The proposed electrode was prepared by one-step electrodeposition of reduced graphene oxide (ERGO) at a GC electrode. Compared with the bare GC electrode, the introduced graphene film on the electrode surface dramatically improved the sensitivity of the sensor response due to the π-π interaction between the graphene and trans-resveratrol...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28819389/discovery-of-non-invasive-glycan-biomarkers-for-detection-and-surveillance-of-gastric-cancer
#11
Ruihuan Qin, Junjie Zhao, Wenjun Qin, Zejian Zhang, Ran Zhao, Jing Han, Yupeng Yang, Lixiao Li, Xuefei Wang, Shifang Ren, Yihong Sun, Jianxin Gu
Purpose: Gastric cancer (GC), one of the world's top five most common cancers, is the third leading cause of cancer related death. It is urgent to identify non-invasive biomarkers for GC. The objective of our study was to find out non-invasive biomarkers for early detection and surveillance of GC based on glycomic analysis. Method: Ethyl esterification derivatization combined with MALDI-TOF MS analysis was employed for the comprehensive serum glycomic analysis in order to investigate glycan markers that would indicate the onset and progression of gastric cancer...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28819379/hypoxia-inducible-factor-1-alpha-correlates-with-tumor-associated-macrophages-infiltration-influences-survival-of-gastric-cancer-patients
#12
Wei-Jie Zhang, Cheng Chen, Zhi-Hua Zhou, Shan-Ting Gao, Teong Jin Tee, Liu-Qing Yang, Yuan-Yuan Xu, Tao-Hong Pang, Xin-Yun Xu, Qi Sun, Min Feng, Hao Wang, Chun-Lei Lu, Guo-Zhong Wu, Sheng Wu, Wen-Xian Guan, Gui-Fang Xu
Background: Hypoxia was a common feature for accelerating tumor metastasis by both inducting epithelial-mesenchymal transition (EMT) of tumor cells and polarization of tumor-associated macrophages (TAMs). The association and roles between hypoxia, EMT and TAMs in the biological behavior of gastric cancer (GC) for the time being recurrence is unclear. Material and methods: hypoixa by expression of hypoxia-inducible factor-1 alpha (HIF-1α), polarized functional status of infiltrated TAMs by immunohistochemical staining of CD68 and CD163, and the expression of E-cadherin as EMT property had been evaluated in 236 patients consecutive with histologically confirmed GC...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28818914/complete-genome-sequence-of-citrobacter-freundii-705sk3-an-oxa-48-encoding-wastewater-isolate
#13
Katrin Zurfluh, Roger Stephan, Jochen Klumpp, Magdalena Nüesch-Inderbinen, Jörg Hummerjohann, Claudia Bagutti, Roger Marti
We present the genome sequence of Citrobacter freundii 705SK3, a wastewater isolate harboring an IncL OXA-48-encoding plasmid. Assembly of the genome resulted in a 5,242,839-bp circular chromosome (GC content, 52%) and two closed plasmids of 296,175 bp and 63, 458 bp in size.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28818895/genome-sequence-of-the-thermophilic-biomass-degrading-fungus-malbranchea-cinnamomea-fch-10-5
#14
Zoraide Granchi, Sake van Pelt, Vu Nguyen Thanh, Lisbeth Olsson, Silvia Hüttner
We report here the annotated draft genome sequence of the thermophilic biomass-degrading fungus Malbranchea cinnamomea strain FCH 10.5, isolated from compost at a waste treatment plant in Vietnam. The genome sequence contains 24.96 Mb with an overall GC content of 49.79% and comprises 9,437 protein-coding genes.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28818802/development-and-validation-of-a-gc-ms-method-for-the-determination-of-hydroxyzine-and-its-active-metabolite-cetirizine-in-whole-blood
#15
Maria Katselou, Sotiris Athanaselis, Panagiota Nikolaou, Artemisia Dona, Chara Spiliopoulou, Ioannis Papoutsis
A simple, rapid, sensitive and accurate gas chromatography-mass spectrometric method was developed and validated for the simultaneous determination of hydroxyzine and cetirizine in whole blood. Solid-phase extraction procedure using Bond Elut LRC Certify II columns was used for the isolation of hydroxyzine and cetirizine from 1mL whole blood followed by derivatization with a mixture of acetic anhydride:n-propanol (1:1, v/v). Limits of detection and quantification were 1.50 and 5.00ng/mL, respectively. The assay was linear within the concentration range of 5...
August 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28818741/identification-of-sterols-from-the-soft-coral-dendronephthya-gigantea-and-their-anti-inflammatory-potential
#16
I P Shanura Fernando, K K Asanka Sanjeewa, Hyun-Soo Kim, Seo-Young Kim, Seung-Hong Lee, Won Woo Lee, You-Jin Jeon
Exploration of anti-inflammatory phytochemicals has received tremendous attention worldwide owing to the rapid increase in inflammatory diseases. Current study reveals the identification of eight 3β-hydroxy-Δ(5)-steroidal congeners from a nonpolar column fraction of the ethanol solubles from the soft coral Dendronephthya gigantea collected from Jeju Island South Korea, using GC-MS/MS analysis. The sterol-rich fraction (DGEH21) showed a significant anti-inflammatory activity as exhibited by the inhibition of NO production (IC50 4...
August 9, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28818100/mir-140-5p-suppresses-the-proliferation-migration-and-invasion-of-gastric-cancer-by-regulating-yes1
#17
Zheng Fang, Shuai Yin, Ruochuan Sun, Shangxin Zhang, Min Fu, Youliang Wu, Tao Zhang, Junaid Khaliq, Yongxiang Li
BACKGROUND: The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-140-5p in regulating tumor progression. METHODS: miR-140-5p level was determined in 20 paired frozen specimens through quantitative real-time PCR, and analyzed in tissue microarrays through in situ hybridization...
August 17, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28817805/ltp-at-hilar-mossy-cell-dentate-granule-cell-synapses-modulates-dentate-gyrus-output-by-increasing-excitation-inhibition-balance
#18
Yuki Hashimotodani, Kaoutsar Nasrallah, Kyle R Jensen, Andrés E Chávez, Daniel Carrera, Pablo E Castillo
Excitatory hilar mossy cells (MCs) in the dentate gyrus receive inputs from dentate granule cells (GCs) and project back to GCs locally, contralaterally, and along the longitudinal axis of the hippocampus, thereby establishing an associative positive-feedback loop and connecting functionally diverse hippocampal areas. MCs also synapse with GABAergic interneurons that mediate feed-forward inhibition onto GCs. Surprisingly, although these circuits have been implicated in both memory formation (e.g., pattern separation) and temporal lobe epilepsy, little is known about activity-dependent plasticity of their synaptic connections...
August 16, 2017: Neuron
https://www.readbyqxmd.com/read/28817591/susceptibility-of-bovine-dental-enamel-with-initial-erosion-lesion-to-new-erosive-challenges
#19
Gabriela Cristina de Oliveira, Guida Paola Genovez Tereza, Ana Paula Boteon, Brunna Mota Ferrairo, Priscilla Santana Pinto Gonçalves, Thiago Cruvinel da Silva, Heitor Marques Honório, Daniela Rios
This in vitro study evaluated the impact of initial erosion on the susceptibility of enamel to further erosive challenge. Thirty bovine enamel blocks were selected by surface hardness and randomized into two groups (n = 15): GC- group composed by enamel blocks without erosion lesion and GT- group composed by enamel blocks with initial erosion lesion. The baseline profile of each block was determined using the profilometer. The initial erosion was produced by immersing the blocks into HCl 0.01 M, pH 2.3 for 30 seconds, under stirring...
2017: PloS One
https://www.readbyqxmd.com/read/28817541/titers-of-antibodies-to-the-surface-antigen-of-hepatitis-b-virus-after-vaccination-in-relation-to-immunity-related-gene-variants-a-prospective-study-among-hemodialysis-patients
#20
Elżbieta Jodłowska-Siewert, Paweł P Jagodziński, Alicja E Grzegorzewska
INTRODUCTION    Hemodialysis (HD) patients show a weaker response to hepatitis B virus (HBV) vaccination than the healthy population. Several gene variants were reported to be associated with the levels of antibodies to HBV surface antigen (anti-HBs) after HBV vaccination among healthy individuals. OBJECTIVES    The aim of the study was to determine the effect of immunity-related genes on the maximum anti-HBs antibody levels after vaccination among HD subjects. PATIENTS AND METHODS    This 6-year prospective study included HD patients who were not infected with HBV and underwent HBV vaccination...
August 9, 2017: Polish Archives of Internal Medicine
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