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Infectious laryngotracheitis virus

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https://www.readbyqxmd.com/read/29223788/a-multiplex-real-time-rt-pcr-for-simultaneous-detection-of-four-most-common-avian-respiratory-viruses
#1
Nacira Laamiri, Rim Aouini, Boutheina Marnissi, Abdeljelil Ghram, Issam Hmila
A one-step multiplex real-time reverse transcription-PCR (rRT-PCR) assay was developed for simultaneous detection and quantification of four avian respiratory viruses: avian influenza virus (AIV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and infectious laryngotracheitis virus (ILTV). In comparison with the singleplex rRT-PCR, the specificity, the sensitivity and the reproducibility of the new assay were evaluated and validated using 70 clinical samples. The optimal cutoff point, the corresponding limit of quantification (LoQ) and the limit of detection (LoD) were statistical established based on receiver operating characteristic (ROC) curve analysis...
December 7, 2017: Virology
https://www.readbyqxmd.com/read/29070686/the-infectious-laryngotracheitis-virus-iltv-viral-chemokine-binding-protein-glycoprotein-g-alters-the-transcription-of-key-inflammatory-mediators-in-vitro-and-in-vivo
#2
Mauricio J C Coppo, Joanne M Devlin, Alistair R Legione, Paola K Vaz, Sang-Won Lee, José A Quinteros, James R Gilkerson, Nino Ficorilli, Patrick C Reading, Amir H Noormohammadi, Carol A Hartley
Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that infects chickens, causing upper respiratory tract disease and significant losses to poultry industries worldwide. Glycoprotein G (gG) is a broad range viral chemokine-binding protein conserved amongst most alphaherpesviruses, including ILTV. A number of studies comparing immunological parameters between infection with gG-expressing and gG-deficient ILTV strains have demonstrated that expression of gG is associated with increased virulence, modification of the amount and composition of the inflammatory response and modulation of immune responses towards antibody production and away from cell-mediated immune responses...
October 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29054443/development-of-an-xtag-multiplex-pcr-array-for-the-detection-of-four-avian-respiratory-viruses
#3
Feng Cong, Yujun Zhu, Xiangnan Liu, Xiuzhen Li, Meili Chen, Ren Huang, Pengju Guo
Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 × 10(2)- 3.52 × 10(3)copies/μL. We examined 60 clinical specimens and found 18 positive for respiratory viruses...
October 17, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28939604/genetic-diversity-of-infectious-laryngotracheitis-virus-during-in-vivo-co-infection-parallels-viral-replication-and-arises-from-recombination-hot-spots-within-the-genome
#4
Carlos A Loncoman, Carol A Hartley, Mauricio J C Coppo, Paola K Vaz, Andrés Diaz-Méndez, Glenn F Browning, Maricarmen García, Stephen Spatz, Joanne M Devlin
Recombination is a feature of many alphaherpesviruses that infect people and animals. Infectious laryngotracheitis virus (ILTV - Gallid alphaherpesvirus 1), causes respiratory disease in chickens, resulting in significant production losses in poultry industries worldwide. Natural (field) ILTV recombination is widespread, particularly recombination between attenuated ILTV vaccine strains to create virulent viruses. These virulent recombinants have had a major impact on animal health. Recently, the development of a single nucleotide polymorphism (SNP) genotyping assay for ILTV has helped to understand ILTV recombination under laboratory settings...
September 22, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28665721/esophagitis-and-pharyngitis-associated-with-avian-infectious-laryngotracheitis-in-backyard-chickens-two-cases
#5
Kathleen Sary, Sonia Chénier, Carl A Gagnon, H L Shivaprasad, Doris Sylvestre, Martine Boulianne
Infectious laryngotracheitis (ILT) is a contagious viral respiratory disease of great economic importance for the global poultry industry caused by Gallid herpesvirus 1 (GaHV-1). Lesions of the upper digestive tract caused by this virus have not been reported before. Two small flocks of backyard chickens experienced an outbreak of ILT, one in 2006 and the other in 2014. These birds had typical ILT lesions, characterized by a necrohemorrhagic laryngitis and tracheitis but were also affected by a severe erosive and necrotic esophagitis and pharyngitis...
June 2017: Avian Diseases
https://www.readbyqxmd.com/read/28532159/the-route-of-inoculation-dictates-the-replication-patterns-of-the-infectious-laryngotracheitis-virus-iltv-pathogenic-strain-and-chicken-embryo-origin-ceo-vaccine
#6
Gabriela Beltrán, Susan M Williams, Guillermo Zavala, James S Guy, Maricarmen García
Infectious laryngotracheitis virus (ILTV) has a high proclivity to replicate in the larynx and trachea of chickens causing severe lesions. There is a lack of knowledge on the ability of ILTV to replicate in other respiratory associated tissues apart from in the trachea. The objective of this study was to investigate how tissues that first encounter the virus dictate further sites of viral replication during the lytic stage of infection. Replication patterns of the pathogenic strain 63140 and the chicken embryo origin (CEO) vaccine in the conjunctiva, the Harderian gland, nasal cavity and trachea were evaluated after ocular, oral, intranasal or intratracheal inoculation of specific pathogen-free chickens...
June 19, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28434824/multiple-pathogen-biomarker-detection-using-an-encoded-bead-array-in-droplet-pcr
#7
Prem Kumar Periyannan Rajeswari, Lovisa M Soderberg, Alia Yacoub, Mikael Leijon, Helene Andersson Svahn, Haakan N Joensson
We present a droplet PCR workflow for detection of multiple pathogen DNA biomarkers using fluorescent color-coded Luminex® beads. This strategy enables encoding of multiple singleplex droplet PCRs using a commercially available bead set of several hundred distinguishable fluorescence codes. This workflow provides scalability beyond the limited number offered by fluorescent detection probes such as TaqMan probes, commonly used in current multiplex droplet PCRs. The workflow was validated for three different Luminex bead sets coupled to target specific capture oligos to detect hybridization of three microorganisms infecting poultry: avian influenza, infectious laryngotracheitis virus and Campylobacter jejuni...
August 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28350819/development-and-application-of-a-taqman-single-nucleotide-polymorphism-genotyping-assay-to-study-infectious-laryngotracheitis-virus-recombination-in-the-natural-host
#8
Carlos A Loncoman, Carol A Hartley, Mauricio J C Coppo, Paola K Vaz, Andrés Diaz-Méndez, Glenn F Browning, Sang-Won Lee, Joanne M Devlin
To date, recombination between different strains of the avian alphaherpesvirus infectious laryngotracheitis virus (ILTV) has only been detected in field samples using full genome sequencing and sequence analysis. These previous studies have revealed that natural recombination is widespread in ILTV and have demonstrated that recombination between two attenuated ILTV vaccine strains generated highly virulent viruses that produced widespread disease within poultry flocks in Australia. In order to better understand ILTV recombination, this study developed a TaqMan single nucleotide polymorphism (SNP) genotyping assay to detect recombination between two field strains of ILTV (CSW-1 and V1-99 ILTV) under experimental conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28194527/impairment-of-infectious-laryngotracheitis-virus-replication-by-deletion-of-the-ul-1-gene
#9
M Nadimpalli, S W Lee, J M Devlin, J R Gilkerson, C A Hartley
Infectious laryngotracheitis virus (ILTV) encodes several unique genes, including a pair of unique nuclear proteins UL0 and UL[-1] that are expressed during replication in cell culture. Although the UL0 gene has been shown to be dispensable for replication, the role of UL[-1] has not been elucidated. In this study a deletion mutant of ILTV lacking the UL[-1] gene was constructed using homologous recombination. The coding sequences of the gene were replaced with the gene for enhanced green fluorescent protein and the cytomegalovirus major immediate early promoter element...
June 2017: Archives of Virology
https://www.readbyqxmd.com/read/28151004/evaluation-of-factors-influencing-the-development-of-late-marek-s-disease-virus-induced-immunosuppression-virus-pathotype-and-host-sex
#10
Nik M Faiz, Aneg L Cortes, James S Guy, Oscar J Fletcher, Thomas Cimino, Isabel M Gimeno
Marek's disease virus (MDV) is a herpesvirus that induces lymphoma and immunosuppression in chickens. MDV-induced immunosuppression (MDV-IS) is complex and can be divided into two phases: early-MDV-IS associated with cytolytic infection in the lymphoid organs in chickens lacking maternal antibodies against MDV (MAbs) and late-MDV-IS that appears later in the pathogenesis and occurs even in chickens bearing MAbs. We have recently developed a model to reproduce late-MDV-IS under laboratory conditions. This model evaluates late-MDV-IS indirectly by assessing the effect of MDV infection on the efficacy of infectious laryngotracheitis (ILT) vaccines against challenge with ILT virus...
April 24, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28057380/current-and-future-vaccines-and-vaccination-strategies-against-infectious-laryngotracheitis-ilt-respiratory-disease-of-poultry
#11
Maricarmen García
Infectious laryngotracheitis (ILT) is an economically important respiratory disease of poultry that affects the industry worldwide. Vaccination is the principal tool in the control of the disease. Two types of vaccines, live attenuated and recombinant viral vector, are commercially available. The first generation of GaHV-1 vaccines available since the early 1960's are live viruses, attenuated by continuous passages in cell culture or embryos. These vaccines significantly reduce mortalities and, in particular, the chicken embryo origin (CEO) vaccines have shown to limit outbreaks of the disease...
July 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28052812/newcastle-disease-virus-vectored-infectious-laryngotracheitis-vaccines-protect-commercial-broiler-chickens-in-the-presence-of-maternally-derived-antibodies
#12
Qingzhong Yu, Stephen Spatz, Yufeng Li, Jilong Yang, Wei Zhao, Zhenyu Zhang, Guoyuan Wen, Maricarmen Garcia, Laszlo Zsak
Newcastle disease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoproteins B and D have previously been demonstrated to confer complete clinical protection against virulent ILTV and NDV challenges in naive chickens. We extended this study to assess whether maternally derived antibody (MDA) against NDV and ILTV would interfere with protection in vaccinated broiler chickens. Chickens with a mean NDV MDA hemagglutination inhibition (HI) titer of 6.4 (log2) and detectable ILTV neutralization (VN) antibodies at hatch were vaccinated with rLS/ILTV-gB or rLS/ILTV-gD at 1 or 10day of age (DOA) or on both days...
February 1, 2017: Vaccine
https://www.readbyqxmd.com/read/27924415/detection-of-infectious-bronchitis-virus-793b-avian-metapneumovirus-mycoplasma-gallisepticum-and-mycoplasma-synoviae-in-poultry-in-ethiopia
#13
S Hutton, J Bettridge, R Christley, T Habte, K Ganapathy
A survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the Ethiopian Institute of Agricultural Research (EIAR), located in Debre Zeit, Ethiopia. Oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. A combination of serological and molecular methods was used for detection of pathogens. For the first time in Ethiopia, we report the detection of variant infectious bronchitis virus (793B genotype), avian metapneumovirus subtype B and Mycoplasma synoviae in poultry...
February 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27796116/health-evaluation-of-african-penguins-i-spheniscus-demersus-i-in-southern-africa
#14
Nola J Parsons, Tertius A Gous, Adam M Schaefer, Ralph E T Vanstreels
The African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a rehabilitation centre...
September 20, 2016: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/27756631/presence-of-dna-extracellular-traps-but-not-muc5ac-and-muc5b-mucin-in-mucoid-plugs-casts-of-infectious-laryngotracheitis-virus-iltv-infected-tracheas-of-chickens
#15
Vishwanatha R A P Reddy, Ivan Trus, Hans J Nauwynck
Although it has been speculated that the tracheal obstructions and asphyxiation during acute infectious laryngotracheitis (ILT) are due to mucoid plugs/casts formed by mucus hypersecretion, there are no reports demonstrating this. Hence, in the present study, we first examined if the main respiratory mucins, MUC5AC and MUC5B, are expressed in the mucosae of larynx, trachea and bronchi of mock-inoculated and ILTV infected chickens. Second, the tracheas with plugs/casts were stained for mucins (MUC5AC and MUC5B) and nuclear material (traps)...
January 2, 2017: Virus Research
https://www.readbyqxmd.com/read/27624642/a-survey-for-selected-avian-viral-pathogens-in-backyard-chicken-farms-in-finland
#16
L Pohjola, N Tammiranta, C Ek-Kommonen, T Soveri, M L Hänninen, M Fredriksson Ahomaa, A Huovilainen
Backyard poultry are regaining popularity in Europe and increased interest in the health and management of non-commercial farms has resulted. Furthermore, commercial poultry farm owners have become concerned about the risk represented by contagious avian diseases that nearby backyard poultry could transmit. Fifty-one voluntary backyard chicken farms were visited between October 2012 and January 2013. Blood samples and individual cloacal swabs were collected from 457 chickens. In 44 farms (86%), one or more of the tested chickens had antibodies against avian encephalomyelitis and chicken infectious anaemia viruses, 24 farms (47%) had chickens seropositive for infectious bronchitis virus, 10 farms (20%) had chickens seropositive for infectious bursal disease virus, six farms (12%) had chickens seropositive for infectious laryngotracheitis virus and two farms (5...
April 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/27558184/accurate-detection-of-avian-respiratory-viruses-by-use-of-multiplex-pcr-based-luminex-suspension-microarray-assay
#17
Nacira Laamiri, Pia Fällgren, Siamak Zohari, Jaouher Ben Ali, Abdeljelil Ghram, Mikael Leijon, Issam Hmila
A novel oligonucleotide suspension microarray (Luminex microsphere system) was developed for the rapid detection of avian respiratory viruses of major clinical importance. This test was optimized and validated with 70 clinical samples. The developed tool was accurate for high-throughput detection and differentiation of the most important avian respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV), and infectious laryngotracheitis virus (ILTV) in single- and mixed-virus infections...
November 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27553200/viral-proteins-expressed-in-the-protozoan-parasite-eimeria-tenella-are-detected-by-the-chicken-immune-system
#18
Virginia Marugan-Hernandez, Charlotte Cockle, Sarah Macdonald, Elaine Pegg, Colin Crouch, Damer P Blake, Fiona M Tomley
BACKGROUND: Eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins in Eimeria species has opened up the possibility of using Eimeria live vaccines to deliver heterologous antigens and function as multivalent vaccine vectors that could protect chickens against a range of pathogens...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27487535/-cytokine-profile-in-young-children-with-acute-stenotic-laryngotracheitis
#19
Ольга І Гладченко, Павло В Токарєв, Олександр Б Надрага
INTRODUCTION: One of the most severe complications of acute respiratory infections in young children is acute stenotic laryngotracheitis (croup). The relationship between cytokine blood levels and symptoms of croup, croup severity, disease sequel, despite numerous studies is still unclear. AIM: Cytokine profile in young children with acute stenotic laryngotracheitis investigation. MATERIAL AND METHODS: 112 children aged 12 min. - 36 mon. with acute stenotic laryngotracheitis which were treated at the Lviv Regional Infectious Diseases Hospital were kept under observation...
2016: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/27371103/efficacy-of-various-marek-s-disease-vaccines-protocols-for-prevention-of-marek-s-disease-virus-induced-immunosuppression
#20
Nik M Faiz, Aneg L Cortes, James S Guy, Jonathan E Fogle, Isabel M Gimeno
Marek's disease virus (MDV) induces tumors and severe immunosuppression in chickens. MDV-induced immunosuppression (MDV-IS) is very complex and difficult to study. In particular, the late MDV-IS (late-MDV-IS) is of great concern since it can occur in the absence of lymphoid organ atrophy or gross tumors. We have recently developed a model to reproduce late-MDV-IS under laboratory conditions. This model measures MDV-IS indirectly by assessing the effect of MDV infection on the efficacy of infectious laryngotracheitis (ILT) vaccination; hence the name late-MDV-IS ILT model...
July 29, 2016: Vaccine
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