keyword
MENU ▼
Read by QxMD icon Read
search

bile duct cell

keyword
https://www.readbyqxmd.com/read/29675965/il-33-overexpression-reflects-less-aggressive-tumour-features-in-large-duct-type-cholangiocarcinomas
#1
Ryuichiro Sawada, Yuna Ku, Masayuki Akita, Kyoko Otani, Kohei Fujikura, Tomoo Itoh, Tetsuo Ajiki, Takumi Fukumoto, Yoshihiro Kakeji, Yoh Zen
BACKGROUND & AIMS: The present study aimed to elucidate the clinicopathological significance of IL-6 and IL-33 expression in intrahepatic cholangiocarcinomas (iCCAs) and perihilar cholangiocarcinomas (pCCAs). METHODS: IL-6 and IL-33 mRNA expression was examined in iCCAs (n=55) and pCCAs (n=32) using quantitative real-time PCR and a highly sensitive in situ hybridization protocol (RNAscope® ), and expression values were correlated with clinicopathological features...
April 19, 2018: Histopathology
https://www.readbyqxmd.com/read/29663481/the-ampk-v-atpase-ph-axis-a-key-regulator-of-the-pro-fibrogenic-phenotype-of-human-hepatic-stellate-cells
#2
Giusi Marrone, Francesco De Chiara, Katrin Böttcher, Ana Levi, Dipok Dhar, Lisa Longato, Giuseppe Mazza, Zhenzhen Zhang, Martina Marrali, Anabel Fernández- Iglesias, Andrew Hall, Tu Vinh Luong, Benoit Viollet, Massimo Pinzani, Krista Rombouts
Liver fibrosis and cirrhosis are characterized by activation of hepatic stellate cells (HSC) which is associated with higher intracellular pH (pHi). The vacuolar H+ adenosine-tri-phosphatase (v-ATPase) multi-subunit complex is a key regulator of intracellular pH homeostasis. The present work was aimed at investigating the functional role of v-ATPase in primary human HSC (hHSC) activation and its modulation by specific AMPK subunits. Here, we demonstrated that the expression of different v-ATPase subunits was increased in in vivo and in vitro activated hHSC, compared to non-activated hHSC...
April 17, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29658929/partial-bile-duct-ligation-in-the-mouse-a-controlled-model-of-localized-obstructive-cholestasis
#3
Shinichiro Yokota, Yoshihiro Ono, Toshimasa Nakao, Peng Zhang, George K Michalopoulos, Zahida Khan
In rodents, complete bile duct ligation (cBDL) of the common bile duct is an established surgical technique for studying obstructive cholestasis and bile duct proliferation. However, long-term experiments can lead to increased morbidity and mortality. In select mouse strains with underlying liver disease, meaningful comparisons can be made even with ligation of a single lobe of the liver, which can reduce animal losses and expenses. Here, we describe partial bile duct ligation (pBDL) in the mouse, in which only the left hepatic bile duct is ligated, causing biliary obstruction in the left lobe but not the remaining lobes...
March 28, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29624127/depolarized-hepatocytes-express-the-stem-progenitor-cell-marker-neighbor-of-punc-e11-after-bile-duct-ligation-in-mice
#4
Andrea Bowe, Susanne Zweerink, Vera Mück, Vangelis Kondylis, Sigrid Schulte, Tobias Goeser, Dirk Nierhoff
There is a medical need of biomarkers for disease stratification in cholestatic liver diseases that come along with changes in hepatocyte polarity. Neighbor of Punc E11 (Nope) is an oncofetal marker that is lost after final differentiation and polarization of hepatocytes. We analyzed the expression pattern of Nope and connexin (Cx) 26 as markers of hepatocyte polarization during murine liver development as well as in adult liver with or without bile duct ligation (BDL) by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), western blotting (WB), and immunohistochemistry...
April 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29619414/hilar-cholangiocarcinoma-associated-with-immunoglobulin-g4-positive-plasma-cells-and-elevated-serum-immunoglobulin-g4-levels
#5
Nabeel Azeem, Veeral Ajmera, Bilal Hameed, Neil Mehta
Immunoglobulin G4 (IgG4)-related disease is a fibroinflammatory systemic disorder with multiorgan involvement. Proximal bile duct involvement results in IgG4-related sclerosing cholangitis, which is characterized by a lymphoplasmacytic infiltrate with abundant IgG4-positive plasma cells and fibrosis. Differentiating between cholangiocarcinoma and IgG4-sclerosing cholangitis can present a diagnostic dilemma. We describe an unusual presentation of a hepatic mass meeting multiple criteria for IgG4-sclerosing cholangitis but was ultimately found to be cholangiocarcinoma...
April 2018: Hepatology Communications
https://www.readbyqxmd.com/read/29615468/intrahepatic-bile-ducts-guide-establishment-of-the-intrahepatic-nerve-network-in-developing-and-regenerating-mouse-liver
#6
Naoki Tanimizu, Norihisa Ichinohe, Toshihiro Mitaka
Epithelial organs consist of multiple tissue structures such as epithelial sheets, blood vessels, and nerves, which are spatially organized to achieve optimal physiological functions. The hepatic nervous system has been implicated in physiological functions and regeneration of the liver. However, the processes of development and reconstruction of the intrahepatic nerve network and its underlying mechanisms remain unknown. Here we demonstrate that class III β-tubulin (TUBB3)+ nerve fibers are not distributed in intrahepatic tissue at embryonic day 17...
April 3, 2018: Development
https://www.readbyqxmd.com/read/29607953/a-rare-case-of-ampullary-goblet-cell-carcinoid
#7
Hitoshi Shibuya, Susumu Hijioka, Nobumasa Mizuno, Takamichi Kuwahara, Nozomi Okuno, Tsutomu Tanaka, Makoto Ishihara, Yutaka Hirayama, Sachiyo Oonishi, Yoshiko Murakami, Yasushi Yatabe, Masahiro Tajika, Yasumasa Niwa, Kazuo Hara
An asymptomatic 70-year-old woman was referred to our hospital because of liver enzyme elevation. Enhanced abdominal computed tomography demonstrated a small, round-shaped tumor with dilation of the common bile duct (CBD) and main pancreatic duct (MPD). A biopsy specimen from the papilla showed mucin-containing cells that were positive for endocrine markers on immunohistochemical staining. Endoscopic snare resection was done, and there was a positive vertical margin on pathology. Pancreaticoduodenectomy was then performed later...
March 30, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29606616/direct-conversion-of-mouse-fibroblasts-into-cholangiocyte-progenitor-cells
#8
Kyung Tae Lim, Jonghun Kim, Seon In Hwang, Ludi Zhang, Heonjong Han, Dasom Bae, Kee-Pyo Kim, Yi-Ping Hu, Hans R Schöler, Insuk Lee, Lijian Hui, Dong Wook Han
Disorders of the biliary epithelium, known as cholangiopathies, cause severe and irreversible liver diseases. The limited accessibility of bile duct precludes modeling of several cholangiocyte-mediated diseases. Therefore, novel approaches for obtaining functional cholangiocytes with high purity are needed. Previous work has shown that the combination of Hnf1β and Foxa3 could directly convert mouse fibroblasts into bipotential hepatic stem cell-like cells, termed iHepSCs. However, the efficiency of converting fibroblasts into iHepSCs is low, and these iHepSCs exhibit extremely low differentiation potential into cholangiocytes, thus hindering the translation of iHepSCs to the clinic...
March 29, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29606134/tgr5-expression-in-normal-kidney-and-renal-neoplasms
#9
Chaohui Lisa Zhao, Ali Amin, Yiang Hui, Dongfang Yang, Weibiao Cao
BACKGROUND: The G protein-coupled bile acid receptor (TGR5) is a cell surface receptor which induces the production of intracellular cAMP and promotes epithelial-mesenchymal transition in gastric cancer cell lines. TGR5 is found in a wide variety of tissues including the kidney. However, the patterns of TGR5 expression have not been well characterized in physiologic kidney or renal neoplasms. We explore the expression of TGR5 in benign renal tissue and renal neoplasms and assess its utility as a diagnostic marker...
April 2, 2018: Diagnostic Pathology
https://www.readbyqxmd.com/read/29603385/downregulated-eosinophil-activity-in-ulcerative-colitis-with-concomitant-primary-sclerosing-cholangitis
#10
Maria Lampinen, Annika Fredricsson, Johan Vessby, Johana Fernandez Martinez, Alkwin Wanders, Fredrik Rorsman, Marie Carlson
Primary sclerosing cholangitis (PSC) is a chronic bile duct inflammation strongly connected to ulcerative colitis (UC). PSC is associated with an increased risk of colon cancer, but the link between the intestinal and the bile duct inflammation is still unknown. Also, the involvement of intestinal immune cells in the pathogenesis of PSC remains to be determined. The eosinophil granulocyte is one of the immune cells implicated in the inflammatory process of ulcerative colitis. This study was performed to determine how the accumulation and activation of intestinal eosinophils may differ between UC with and without concomitant PSC, and how this may be influenced by the cytokine/chemokine profile of the intestinal compartment...
March 30, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29601088/blocking-h1-h2-histamine-receptors-inhibits-damage-fibrosis-in-mdr2-mice-and-human-cholangiocarcinoma-tumorigenesis
#11
Lindsey Kennedy, Laura Hargrove, Jennifer Demieville, Allen Karstens, Hannah Jones, Sharon DeMorrow, Fanyin Meng, Pietro Invernizzi, Francesca Bernuzzi, Gianfranco Alpini, Steven Smith, Austin Akers, Victoria Meadows, Heather Francis
BACKGROUND: Primary Sclerosing Cholangitis (PSC) patients are at risk of developing cholangiocarcinoma (CCA). We have shown that: (i) histamine (HA) increases biliary hyperplasia via H1/H2 histamine receptors (HRs) and (ii) HA levels increase and mast cells (MCs) infiltrate during PSC and CCA. We aimed to examine the effects of chronic treatment with H1/H2HR antagonists on PSC and CCA. METHODS: Wild-type and Mdr2-/- mice were treated by osmotic minipumps with saline, mepyramine or ranitidine (10mg/kg BW/day) or a combination of mepyramine/ranitidine for 4 wks...
March 30, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29596214/natural-history-and-prognostic-factors-of-cholangiocarcinoma-with-spinal-metastasis-a-10-year-single-center-study
#12
Apiruk Sangsin, Dew Saiudom, Suthipas Pongmanee, Jirawat Saengsin, Taninnit Leerapun, Hideki Murakami
STUDY DESIGN: This is a retrospective analysis. OBJECTIVE: The aim of this study was to determine the epidemiology, survival, and prognostic factors for cholangiocarcinoma (CCA) with spinal metastasis. SUMMARY OF BACKGROUND DATA: CCA is an epithelial cell malignancy of the bile duct, and a frequent site for its metastasis is the spine. Many areas of Asia are endemic for CCAs. To date, there is limited data on the epidemiology, natural history, and prognostic factors of CCA with spinal metastasis, which is crucial for better management and treatment of the disease...
April 2018: Clinical Spine Surgery
https://www.readbyqxmd.com/read/29594193/expression-of-intrahepatic-cd3-cd4-and-cd8-t-cells-in-biliary-atresia
#13
Behairy E Behairy, Nermine Ehsan, Magdy Anwer, Alif Allam, Ibramem El-Henawy, Nesreen Abdel Hameed, Haidy M Zakaria
Aim of the study: Assessment of the expression of cluster of differentiation (CD)3, CD4, and CD8 T cells in biliary atresia (BA) cases in comparison to neonatal cholestasis other than BA. Material and methods: This study included 79 patients: 34 patients with BA (BA group) and 35 patients with neonatal cholestasis due to causes other than BA (cholestasis group), and 10 normal liver donor as a control group. Immunohistochemical staining or CD3, CD4, and CD8 T cells in liver tissues for the 3 groups were evaluated...
March 2018: Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/29588511/structure-and-distribution-of-an-unrecognized-interstitium-in-human-tissues
#14
Petros C Benias, Rebecca G Wells, Bridget Sackey-Aboagye, Heather Klavan, Jason Reidy, Darren Buonocore, Markus Miranda, Susan Kornacki, Michael Wayne, David L Carr-Locke, Neil D Theise
Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of human tissues at a depth of 60-70 μm during endoscopy. pCLE of the extrahepatic bile duct after fluorescein injection demonstrated a reticular pattern within fluorescein-filled sinuses that had no known anatomical correlate. Freezing biopsy tissue before fixation preserved the anatomy of this structure, demonstrating that it is part of the submucosa and a previously unappreciated fluid-filled interstitial space, draining to lymph nodes and supported by a complex network of thick collagen bundles...
March 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29582676/factors-impacting-the-performance-characteristics-of-bile-duct-brushings-a-clinico-cytopathologic-analysis-of-253-patients
#15
Ezgi Hacihasanoglu, Bahar Memis, Burcin Pehlivanoglu, Vaidehi Avadhani, Alexa A Freedman, Michael M Goodman, N Volkan Adsay, Michelle D Reid
CONTEXT: - Literature on factors impacting bile duct brushings (BDBs) performance characteristics remain limited. OBJECTIVE: - To capture the current state of daily practice with BDB sign-out. DESIGN: - Two hundred fifty-three of 444 BDBs signed out by more than 7 cytopathologists, with histopathologic and/or clinical follow-up of at least 18 months, were examined. RESULTS: - One hundred thirty-five of 253 BDBs (53%) had histologically confirmed malignancies, 22 (9%) had cancer-related deaths, and 96 (38%) were benign...
March 27, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29580319/liver-and-pancreas-do-similar-embryonic-development-and-tissue-organization-lead-to-similar-mechanisms-of-tumorigenesis
#16
Elsa Ghurburrun, Ivan Borbath, Frédéric P Lemaigre, Patrick Jacquemin
Liver and pancreas are closely associated organs which share a common embryological origin. They display amphicrine properties and have similar exocrine organization with parenchymal cells, namely hepatocytes and acinar cells, secreting bile and pancreatic juice into the duodenum via a converging network of bile ducts and pancreatic ducts. Here we compare and highlight the similarities of molecular mechanisms leading to liver and pancreatic cancer development. We suggest that unraveling tumor development in an organ may provide insight into our understanding of carcinogenesis in the other organ...
March 26, 2018: Gene Expression
https://www.readbyqxmd.com/read/29580318/%C3%AE-7-nachr-knockout-mice-decreases-biliary-hyperplasia-and-liver-fibrosis-in-cholestatic-bile-duct-ligated-mice
#17
Laurent Ehrlich, April O'Brien, Chad Hall, Tori White, Lixian Chen, Nan Wu, Julie Venter, Marinda Scrushy, Muhammad Mubarak, Fanyin Meng, David Dostal, Chaodong Wu, Terry C Lairmore, Gianfranco Alpini, Shannon Glaser
α7-nAChR is a nicotinic acetylcholine receptor (specifically expressed on hepatic stellate cells, Kupffer cells, and cholangiocytes) that regulates inflammation and apoptosis in the liver. Thus, targeting α7-nAChR may be therapeutic in biliary diseases. METHODS: Bile-duct ligation (BDL) was performed on wild-type (WT) and α7-nAChR-/- mice. We first evaluated the expression of α7-nAChR by immunohistochemistry (IHC) in liver sections. IHC was also performed to assess intrahepatic bile-duct mass (IBDM), and Sirius Red staining was performed to quantify the amount of collagen deposition...
March 26, 2018: Gene Expression
https://www.readbyqxmd.com/read/29574153/inhibition-of-klhl21-prevents-cholangiocarcinoma-progression-through-regulating-cell-proliferation-and-motility-arresting-cell-cycle-and-reducing-erk-activation
#18
Jian Chen, Wenfeng Song, Yehui Du, Zequn Li, Zefeng Xuan, Long Zhao, Jun Chen, Yongchao Zhao, Biguang Tuo, Shusen Zheng, Penghong Song
Kelch-like family member 21 (KLHL21) is involved in cell mitosis and motility. Nevertheless, the clinical significance and biological function of KLHL21 in cholangiocarcinoma (CCA) are elusive. This is the first study to describe a pivotal role for KLHL21 in the progression of CCA. The expression of KLHL21 was elevated in CCA tissues compared with paired normal bile duct tissues. In addition, immunohistochemical and statistical analyses demonstrated that the expression of KLHL21 correlated inversely with tumor histological grade (p < 0...
March 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29572892/sphingosine-kinase-1-promotes-liver-fibrosis-by-preventing-mir-19b-3p-mediated-inhibition-of-ccr2
#19
Tian Lan, Changzheng Li, Guizhi Yang, Yue Sun, Lihang Zhuang, Yitao Ou, Hui Li, Genshu Wang, Tatiana Kisseleva, David Brenner, Jiao Guo
Chronic liver disease mediated by activation of hepatic stellate cells (HSCs) and Kupffer cells (KCs) leads to liver fibrosis. Here, we aimed to investigate the molecular mechanism and define the cell type involved in mediating the SphK1-dependent effect on liver fibrosis. The levels of expression and activity of SphK1 were significantly increased in fibrotic livers compared with the normal livers in human. SphK1 was co-expressed with a range of HSC/KC markers including desmin, α-smooth muscle actin (α-SMA) and F4/80 in fibrotic liver...
March 23, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29562907/tumor-infiltrating-mast-cells-predict-prognosis-and-gemcitabine-based-adjuvant-chemotherapeutic-benefit-in-biliary-tract-cancer-patients
#20
Xiaobo Bo, Jie Wang, Tao Suo, Xiaoling Ni, Han Liu, Sheng Shen, Min Li, Yueqi Wang, Houbao Liu, Jiejie Xu
BACKGROUND: Recent studies have reported TIMs play an important role in tumors progression or regression, but the effect of TIMs in biliary tract cancer remains unclear. The aim of this study is to investigate the prognostic value of tumor infiltrating mast cells (TIMs) and its influence on gemcitabine-based adjuvant chemotherapy (ACT) benefits in biliary tract cancer patients after surgery. METHODS: TIMs were evaluated by immunohistochemical staining of tryptase in 250 patients with resected gallbladder carcinoma (GBC) or extrahepatic bile duct carcinoma (EBDC) from Zhongshan Hospital...
March 21, 2018: BMC Cancer
keyword
keyword
77193
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"