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https://www.readbyqxmd.com/read/28088116/upconversion-color-tuning-in-ce-3-doped-liyf4-yb-3-ho-3-liyf4-nanoparticles-towards-ratiometric-fluorescence-detection-of-chromium-iii
#1
Shijiang Liu, Yangjie Li, Cheng Zhang, Liang Yang, Tingting Zhao, Ruilong Zhang, Changlong Jiang
Ratiometric fluorescence sensor exhibits advantages of sensitive response, high anti-interference ability and naked eye visualization owing to multiple independent emission peaks utilized for results analysis. To achieve such multi-emission probe, the traditional methods have involved simple mixing of two emitters or tedious synthesis processes of hybrid material. However, these probes often have problems of inconstant emission strength ratio, low light-stability, and complicated synthetic process, which limit their applications in practical field...
January 7, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28088110/highly-sensitive-detection-of-cysteine-over-glutathione-and-homo-cysteine-new-insight-into-the-michael-addition-of-mercapto-group-to-maleimide
#2
Zhaoyang Chen, Qian Sun, Yuhua Yao, Xiaoxiang Fan, Weibing Zhang, Junhong Qian
A fluorescence "off-on" probe CMP for thiols was designed with coumarin as the fluorophore and maleimide as the receptor. The fluorescence of the coumarin was quenched through photoinduced electron transfer (PET) from the fluorophore to maleimide group. The Michael addition of the mercapto group toward maleimide formed a thioether with relatively weak fluorescence. The intramolecular nucleophilic substitution of amino group in cysteine (Cys) to alkylthio produced a much stronger fluorescent amino adduct, which was supported by UPLC-MS and NMR titration...
January 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28088092/aptamer-based-environmental-biosensors-for-small-molecule-contaminants
#3
REVIEW
Van-Thuan Nguyen, Young Seop Kwon, Man Bock Gu
Aptasensors are promising biosensors, which take advantage of using aptamers as a recognition element. The combination of the excellent characteristics of aptamers and the leading detection platform techniques, such as optical, electrochemical with nanomaterial-integrated, or mass-sensitive techniques with high sensitivity and specificity draws a promising view for the application of the aptasensors for the detection of harmful small toxic chemicals and real-time monitoring in the environments. In spite of attraction of aptasensors, application of them is limited to the complex environment due to the facts that how the immobilization of aptamers onto the surface affects the functions of aptamers and their structures for the detection of environmental contaminants are not clearly known...
January 11, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28088052/positive-and-negative-life-events-and-reasons-for-living-modulate-suicidal-ideation-in-a-sample-of-patients-with-history-of-suicide-attempts
#4
Camélia Laglaoui Bakhiyi, Isabelle Jaussent, Séverine Beziat, Renaud Cohen, Catherine Genty, Jean-Pierre Kahn, Marion Leboyer, Pascal Le Vaou, Sébastien Guillaume, Philippe Courtet
BACKGROUND: The influence of life events on suicidal behavior remains inconclusive, while reasons for living (RFL) may be protective. OBJECTIVES: To analyze the association between positive and negative life events and suicidal ideation (SI) and the interaction between life events and RFL on SI. METHOD: Patients with history of suicide attempts (n = 338) underwent a comprehensive clinical evaluation, including SI (Beck's Suicidal Ideation scale), RFL (Reasons for Living Inventory, RFLI) and life events (family, school, student or professional, social, health and religion-related and other life events) during the last twelve months...
January 1, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28088043/hplc-uv-method-for-simultaneous-determination-of-mk-1775-and-azd-7762-in-both-acetonitrile-aqueous-solution-and-mouse-plasma
#5
REVIEW
Kareem Ebeid, Giang N Ho, Aliasger K Salem
A sensitive and precise method is described for the simultaneous determination of two small molecule kinase inhibitors: MK-1775 (MK) and AZD-7762 (AZD), in acetonitrile (ACN)-aqueous solution and in mouse plasma. A Nova-Pak C18 reversed phase column (3.9mm×150mm, 4μm, 60Å) was utilized in the separation using an isocratic mobile phase of 0.1% v/v triethylamine in phosphate buffer (pH=7.4): acetonitrile (ACN) (60:40, v/v), at a flow rate of 0.8mL/min. Detection wavelength was set at 310nm for both MK and AZD, and 431nm for the internal standard sunitinib (SUN)...
December 30, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28087974/application-of-z-sinapinic-matrix-in-peptide-maldi-ms-analysis
#6
María L Salum, Silvana L Giudicessi, Tobías Schmidt De León, Silvia A Camperi, Rosa Erra-Balsells
Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices successful application of matrix assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here Z-SA as matrix for peptides is studied and compared with E-SA and CHCA. Minor number of clusters are always observed in the low m/z region allowing the detection of short peptides...
January 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28087838/does-the-assessment-of-non-disjunction-provide-a-more-sensitive-assay-for-detection-of-aneugens
#7
Joanne M Elloway, Alexandra K Davies, Julie Hayes, Ann T Doherty
The detection of aneugenic chemicals is important due to the implications of aneuploidy for human health. Aneuploidy can result from chromosome loss or non-disjunction due to chromosome mis-segregation at anaphase. Frequently, aneugens are detected using the in vitro micronucleus assay (IVM), with either centromere or kinetochore labelling. However, this method does not consider non-disjunction, the suggested predominant mechanism of spindle poison induced aneugenicity in primary human lymphocytes. Therefore, the IVM may be relatively insensitive in detecting aneuploidy...
January 13, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28087818/three-hours-of-intermittent-hypoxia-increases-circulating-glucose-levels-in-healthy-adults
#8
Lauren P Newhouse, Michael J Joyner, Timothy B Curry, Marcello C Laurenti, Chiara Dalla Man, Claudio Cobelli, Adrian Vella, Jacqueline K Limberg
An independent association exists between sleep apnea and diabetes. Animal models suggest exposure to intermittent hypoxia, a consequence of sleep apnea, results in altered glucose metabolism and fasting hyperglycemia. However, it is unknown if acute exposure to intermittent hypoxia increases glucose concentrations in nondiabetic humans. We hypothesized plasma glucose would be increased from baseline following 3 h of intermittent hypoxia in healthy humans independent of any effect on insulin sensitivity. Eight (7M/1F, 21-34 years) healthy subjects completed two study visits randomized to 3 h of intermittent hypoxia or continuous normoxia, followed by an oral glucose tolerance test...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28087778/no-evidence-for-phylostratigraphic-bias-impacting-inferences-on-patterns-of-gene-emergence-and-evolution
#9
Tomislav Domazet-Lošo, Anne-Ruxandra Carvunis, M Mar Albà, Martin Sebastijan Šestak, Robert Bakarić, Rafik Neme, Diethard Tautz
Phylostratigraphy is a computational framework for dating the emergence of DNA and protein sequences in a phylogeny. It has been extensively applied to make inferences on patterns of genome evolution, including patterns of disease gene evolution, ontogeny and de novo gene origination. Phylostratigraphy typically relies on BLAST searches along a species tree, but new simulation studies have raised concerns about the ability of BLAST to detect remote homologues and its impact on phylostratigraphic inferences...
January 12, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28087758/method-for-the-determination-of-carbonyl-compounds-in-e-cigarette-aerosols
#10
Jason W Flora, Celeste T Wilkinson, James W Wilkinson, Peter J Lipowicz, James A Skapars, Adam Anderson, John H Miller
Low levels of thermal degradation products such as carbonyls (formaldehyde, acetaldehyde, acrolein, crotonaldehyde) have been reported in e-cigarette aerosols. The collection and analysis of e-cigarette aerosol carbonyls are often adapted from methods developed for tobacco cigarette smoke. These methodologies are often not sensitive enough to detect low carbonyl levels in e-cigarette aerosols. One objective of this work was to develop and validate a rapid, selective and sensitive ultra-performance liquid chromatography with mass spectrometry method optimized for analysis of carbonyls in e-cigarette aerosols...
February 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28087616/pathways-to-false-positive-diagnoses-using-molecular-genetic-detection-methods-phytophthora-cinnamomi-a-case-study
#11
Manisha Kunadiya, Diane White, William A Dunstan, Giles E St, J Hardy, Vera Andjic, Treena I Burgess
Phytophthora cinnamomi is one of the world's most invasive plant pathogens affecting ornamental plants, horticultural crops and natural ecosystems. Accurate diagnosis is very important to determine the presence or absence of this pathogen in diseased and asymptomatic plants. In previous studies, P. cinnamomi species-specific primers were designed and tested using various polymerase chain reaction (PCR) techniques including conventional PCR, nested PCR, and quantitative real time PCR (qPCR). In all cases, the primers were stated to be highly specific and sensitive to P...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28087578/distinct-binding-of-pet-ligands-pbb3-and-av-1451-to-tau-fibril-strains-in-neurodegenerative-tauopathies
#12
Maiko Ono, Naruhiko Sahara, Katsushi Kumata, Bin Ji, Ruiqing Ni, Shunsuke Koga, Dennis W Dickson, John Q Trojanowski, Virginia M-Y Lee, Mari Yoshida, Isao Hozumi, Yasumasa Yoshiyama, John C van Swieten, Agneta Nordberg, Tetsuya Suhara, Ming-Rong Zhang, Makoto Higuchi
Diverse neurodegenerative disorders are characterized by deposition of tau fibrils composed of conformers (i.e. strains) unique to each illness. The development of tau imaging agents has enabled visualization of tau lesions in tauopathy patients, but the modes of their binding to different tau strains remain elusive. Here we compared binding of tau positron emission tomography ligands, PBB3 and AV-1451, by fluorescence, autoradiography and homogenate binding assays with homologous and heterologous blockades using tauopathy brain samples...
January 12, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/28087261/dna-aptamer-probes-for-detection-of-estrogen-receptor-%C3%AE-positive-carcinomas
#13
Arghya Sett, Bibhuti Bhusan Borthakur, Jagannath Dev Sharma, Amal Chandra Kataki, Utpal Bora
Estrogen receptor alpha (ERα) which is a ligand-activated transcription factor also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1). It is an important biomarker for breast cancer metastasis. In the present study, we report a novel DNA aptamer candidate against estrogen receptor (ER) alpha structure. The enriched aptamer candidate was obtained after 14 iterative cycles of in vitro protein-SELEX process. Isothermal calorimetry study suggests the nanomolar sensitivity of the candidate ER_Apt1 to its target protein...
December 25, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28087157/the-association-of-blood-urea-nitrogen-levels-with-mortality-in-acute-pulmonary-embolism
#14
Mustafa A Tatlisu, Adnan Kaya, Muhammed Keskin, Sahin Avsar, Mehmet Bozbay, Kiymet Tatlisu, Mehmet Eren
BACKGROUND: The aim of this study was to investigate the association of BUN levels with in-hospital and long-term adverse clinical outcomes in acute pulmonary embolism (APE) patients treated with tissue-plasminogen activator (t-PA). METHODS: This retrospective study included 252 consecutive confirmed APE patients treated with t-PA. An admission BUN of 34.5 mg/dL was identified through an ROC analysis as an optimal cutoff value to predict the in-hospital mortality with 85% sensitivity and 91% specificity (AUC: 0...
December 29, 2016: Journal of Critical Care
https://www.readbyqxmd.com/read/28087154/evaluation-of-serial-high-sensitivity-troponin-t-levels-in-individuals-without-overt-coronary-heart-disease-following-exercise-stress-testing
#15
Yousef M E Saad, Hanan Idris, Ibrahim M Shugman, Krishna K Kadappu, Rohan Rajaratnam, Liza Thomas, Christian Mussap, Dominic Y C Leung, Craig P Juergens, John K French
BACKGROUND: Detectable levels of high sensitivity (cardiac) troponin T (HsTnT), occur in the majority of patients with stable coronary heart disease (CHD), and often in 'healthy' individuals. Extreme physical activity may lead to marked elevations in creatine kinase MB and TnT levels. However, whether HsTnT elevations occur commonly after exercise stress testing (EST), and if so, whether this has clinical significance, needs clarification. METHODS: To determine whether HsTnT levels become elevated after EST (Bruce protocol) to ≥95% of predicted maximum heart rate in presumed healthy subjects without overt CHD, we assayed HsTnT levels for ∼5h post-EST in 105 subjects (median age 37 years)...
December 9, 2016: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28086963/near-infrared-spectroscopy-for-body-fat-sensing-in-neonates-quantitative-analysis-by-gamos-simulations
#16
Fatin Hamimi Mustafa, Peter W Jones, Alistair L McEwan
BACKGROUND: Under-nutrition in neonates is closely linked to low body fat percentage. Undernourished neonates are exposed to immediate mortality as well as unwanted health impacts in their later life including obesity and hypertension. One potential low cost approach for obtaining direct measurements of body fat is near-infrared (NIR) interactance. The aims of this study were to model the effect of varying volume fractions of melanin and water in skin over NIR spectra, and to define sensitivity of NIR reflection on changes of thickness of subcutaneous fat...
January 11, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28086902/exploiting-temporal-and-nonstationary-features-in-breathing-sound-analysis-for-multiple-obstructive-sleep-apnea-severity-classification
#17
Jaepil Kim, Taehoon Kim, Donmoon Lee, Jeong-Whun Kim, Kyogu Lee
BACKGROUND: Polysomnography (PSG) is the gold standard test for obstructive sleep apnea (OSA), but it incurs high costs, requires inconvenient measurements, and is limited by a one-night test. Thus, a repetitive OSA screening test using affordable data would be effective both for patients interested in their own OSA risk and in-hospital PSG. The purpose of this research was to develop a four-OSA severity classification model using a patient's breathing sounds. METHODS: Breathing sounds were recorded from 83 subjects during a PSG test...
January 7, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28086864/direct-detection-of-falciparum-and-non-falciparum-malaria-dna-from-a-drop-of-blood-with-high-sensitivity-by-the-dried-lamp-system
#18
Kyoko Hayashida, Kiichi Kajino, Humphrey Simukoko, Martin Simuunza, Joseph Ndebe, Amos Chota, Boniface Namangala, Chihiro Sugimoto
BACKGROUND: Because of the low sensitivity of conventional rapid diagnostic tests (RDTs) for malaria infections, the actual prevalence of the diseases, especially those caused by non-Plasmodium falciparum (non-Pf) species, in asymptomatic populations remain less defined in countries lacking in well-equipped facilities for accurate diagnoses. Our direct blood dry LAMP system (CZC-LAMP) was applied to the diagnosis of malaria as simple, rapid and highly sensitive method as an alternative for conventional RDTs in malaria endemic areas where laboratory resources are limited...
January 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28086796/prophylaxis-of-post-erc-infectious-complications-in-patients-with-biliary-obstruction-by-adding-antimicrobial-agents-into-erc-contrast-media-a-single-center-retrospective-study
#19
Hella Wobser, Agnetha Gunesch, Frank Klebl
BACKGROUND: Patients with biliary obstruction are at high risk to develop septic complications after endoscopic retrograde cholangiography (ERC). We evaluated the benefits of local application of antimicrobial agents into ERC contrast media in preventing post-ERC infectious complications in a high-risk study population. METHODS: Patients undergoing ERC at our tertiary referral center were retrospectively included. Addition of vancomycin, gentamicin and fluconazol into ERC contrast media was evaluated in a case-control design...
January 13, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28086789/detection-of-plasmodium-knowlesi-plasmodium-falciparum-and-plasmodium-vivax-using-loop-mediated-isothermal-amplification-lamp-in-a-co-endemic-area-in-malaysia
#20
Kim A Piera, Ammar Aziz, Timothy William, David Bell, Iveth J González, Bridget E Barber, Nicholas M Anstey, Matthew J Grigg
BACKGROUND: Plasmodium knowlesi is the most common cause of malaria in Malaysia. However, microscopic diagnosis is inaccurate and rapid diagnostic tests (RDTs) are insufficiently sensitive. PCR is sensitive and specific but not feasible at a district level. Loop-mediated isothermal amplification (LAMP) shows potential with only basic requirements. A commercially available LAMP assay, the Eiken Loopamp™ MALARIA Pan Detection kit, is sensitive for Plasmodium falciparum and Plasmodium vivax, but has not previously been evaluated for P...
January 13, 2017: Malaria Journal
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