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https://www.readbyqxmd.com/read/28343363/sample-pooling-for-real-time-pcr-detection-and-virulence-determination-of-the-footrot-pathogen-dichelobacter-nodosus
#1
Sara Frosth, Ulrika König, Ann-Kristin Nyman, Anna Aspán
Dichelobacter nodosus is the principal cause of ovine footrot and strain virulence is an important factor in disease severity. Therefore, detection and virulence determination of D. nodosus is important for proper diagnosis of the disease. Today this is possible by real-time PCR analysis. Analysis of large numbers of samples is costly and laborious; therefore, pooling of individual samples is common in surveillance programs. However, pooling can reduce the sensitivity of the method. The aim of this study was to develop a pooling method for real-time PCR analysis that would allow sensitive detection and simultaneous virulence determination of D...
March 25, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28343347/bioanalysis-of-farnesyl-pyrophosphate-in-human-plasma-by-high-performance-liquid-chromatography-coupled-to-triple-quadrupole-tandem-mass-spectrometry-and-hybrid-quadrupole-orbitrap-high-resolution-mass-spectrometry
#2
Hiroshi Sugimoto, Mie Iguchi, Fumihiro Jinno
The isoprenoids farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) are pivotal intermediates for cholesterol homeostasis and cell signaling in the mevalonate pathway. We developed a sensitive and selective high-performance liquid chromatography tandem triple quadrupole mass spectrometry (LC-QQQ-MS) method for FPP in human plasma without the need for a derivatization process. We optimized the sample preparation procedure to extract FPP and (13)C5-FPP (as internal standard) from sample fluids using methanol...
March 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28343345/assessment-of-nucleosides-as-putative-tumor-biomarkers-in-prostate-cancer-screening-by-ce-uv
#3
Adriana Zardini Buzatto, Mariana de Oliveira Silva, Ronei Jesus Poppi, Ana Valéria Colnaghi Simionato
Cancer is responsible for millions of deaths worldwide, but most base diseases may be cured if detected early. Screening tests may be used to identify early-stage malignant neoplasms. However, the major screening tool for prostate cancer, the prostate-specific antigen test, has unsuitable sensitivity. Since cancer cells may affect the pattern of consumption and excretion of nucleosides, such biomolecules are putative biomarkers that can be used for diagnosis and treatment evaluation. Using a previously validated method for the analysis of nucleosides in blood serum by capillary electrophoresis with UV-vis spectroscopy detection, we investigated 60 samples from healthy individuals and 42 samples from prostate cancer patients...
March 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28343325/mri-can-accurately-detect-meniscal-ramp-lesions-of-the-knee
#4
Justin W Arner, Elmar Herbst, Jeremy M Burnham, Ashish Soni, Jan-Hendrik Naendrup, Adam Popchak, Freddie H Fu, Volker Musahl
PURPOSE: Posterior horn meniscal tears are commonly found in conjunction with anterior cruciate ligament (ACL) injury. Some believe tears in the posterior meniscocapsular zone, coined ramp lesions, are important to knee stability. The purpose of this study was to determine whether pre-operative MRI evaluation was able to accurately and reproducibly identify ramp lesions. METHODS: Three blinded reviewers assessed MRIs twice for the presence of ramp lesions in patients undergoing ACL reconstruction...
March 25, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28343316/translation-and-validation-of-iciq-fluts-for-tamil-speaking-women
#5
Chanil D Ekanayake, Arunasalam Pathmeswaran, A A Nilanga Nishad, Kanishka U Samaranayake, Prasantha S Wijesinghe
AIMS: Research in to lower urinary tract symptoms (LUTS) in women in South Asia is hampered by lack of validated tools. Our aim was to validate the International Consultation on Incontinence Modular Questionnaire on Female Lower Urinary Tract Symptoms (ICIQ-FLUTS) from English to Tamil. METHOD: After translation to Tamil, a validation study was carried out among women attending the gynecology clinic at District General Hospital-Mannar. RESULTS: Content validity assessed by the level of missing data was <2%...
March 25, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28343299/the-role-of-line-1-methylation-in-predicting-survival-among-colorectal-cancer-patients-a-meta-analysis
#6
Ding Ye, Danjie Jiang, Yingjun Li, Mingjuan Jin, Kun Chen
BACKGROUND: The prognostic value of long interspersed nucleotide element-1 (LINE-1) methylation in patients with colorectal cancer (CRC) remains uncertain. We have therefore performed a meta-analysis to elucidate this issue. METHODS: The PubMed and Web of Science databases were searched for studies published up to 30 June 2016 which reported on an association between LINE-1 methylation and overall survival (OS), disease-free survival (DFS), or cancer-specific survival (CSS) among CRC patients...
March 25, 2017: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28343279/the-independent-influences-of-heat-strain-and-dehydration-upon-cognition
#7
Anne M J van den Heuvel, Benjamin J Haberley, David J R Hoyle, Nigel A S Taylor, Rodney J Croft
PURPOSE: Many researchers have addressed the potential effects of hyperthermia and dehydration on cognition, often revealing contradictory outcomes. A possible reason for this inconsistency is that experiments may have been inadequately designed for such effects. In this study, the impact of hyperthermia, dehydration and their combination on cognition were evaluated in eight young males, after accounting for a range of experimental limitations. METHODS: Passive heating and thermal clamping at two mean body temperatures (36...
March 25, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28343245/an-acid-inert-fluorescent-probe-for-the-detection-of-nitrite
#8
Meiyi Cai, Xiaoyun Chai, Xuedong Wang, Ting Wang
The fluorescent detection of nitrite ions has been challenging owing to the poor acid-stability of typical fluorescent probes. Herein, an acid-inert rhodamine-based fluorescent probe has been developed for the fast detection of trace amount of nitrite ions with turn-on mode. The detection limit of nitrite ions was determined to be 9.4 nM (S/N = 3) and the linear range with high linear correlation was observed between 0.025 to 2.5 μM. A test paper method was developed for rapid visual detection of nitrite ions...
March 25, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28343244/a-new-reactive-1-8-naphthalimide-derivative-for-highly-selective-and-sensitive-detection-of-hg-2
#9
Feng Lv, Yufen Chen, Tengxuan Tang, Yuhua Chen, Dongmei Xu
A 1,8-naphthalimide derivative with a reactive aliphatic hydroxyl was designed and synthesized as a fluorescent probe. Its structure was characterized by IR, (1)H NMR, (13)C NMR, LC-MS and HPLC. The probe showed high selectivity and sensitivity to Hg(2+) over other metal ions such as Pb(2+), Na(+), K(+), Cd(2+), Cr(3+), Zn(2+), Cu(2+), Ni(2+), Ca(2+), Fe(3+), Fe(2+), Co(2+), Mn(2+) and Mg(2+) in MeCN/H2O (15/85, v/v). The increase in fluorescence intensity was linearly proportional to the concentration of Hg(2+) in the range of 18-40 μM with a detection limit of 1...
March 25, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28343143/impact-of-rivaroxaban-on-point-of-care-assays
#10
Pierre Fontana, Lorenzo Alberio, Anne Angelillo-Scherrer, Lars M Asmis, Wolfgang Korte, Adriana Mendez, Pirmin Schmid, Hans Stricker, Jan-Dirk Studt, Dimitrios A Tsakiris, Walter A Wuillemin, Michael Nagler
BACKGROUND: Point-of-care testing (POCT) is regularly used to assess haemostasis in various clinical settings. The impact of rivaroxaban on those POCT is still elusive. We aimed to study the effects of rivaroxaban on most commonly used POCT assays. METHODS: Blood samples were taken before, 3h, and 24h after administration of 20mg rivaroxaban to 20 healthy volunteers as part of a prospective, multicenter validation study (clinicaltrials.govNCT01710267). Blood samples were analysed with thromboelastometry (ROTEM®), two platelet function assays (INNOVANCE® PFA-200 and Multiplate®), and the CoaguChek® XS...
March 21, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28343104/localized-surface-plasmon-resonance-mediated-fluorescence-signals-in-plasmonic-nanoparticle-quantum-dot-hybrids-for-ultrasensitive-zika-virus-rna-detection-via-hairpin-hybridization-assays
#11
Oluwasesan Adegoke, Masahiro Morita, Tatsuya Kato, Masahito Ito, Tetsuro Suzuki, Enoch Y Park
The current epidemic caused by the Zika virus (ZIKV) and the devastating effects of this virus on fetal development, which result in an increased incidence of congenital microcephaly symptoms, have prompted the World Health Organization (WHO) to declare the ZIKV a public health issue of global concern. Efficient probes that offer high detection sensitivity and specificity are urgently required to aid in the point-of-care treatment of the virus. In this study, we show that localized surface plasmon resonance (LSPR) signals from plasmonic nanoparticles (NPs) can be used to mediate the fluorescence signal from semiconductor quantum dot (Qdot) nanocrystals in a molecular beacon (MB) biosensor probe for ZIKV RNA detection...
March 22, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28343103/pd-au-carbon-dots-nanocomposite-facile-synthesis-and-application-as-an-ultrasensitive-electrochemical-biosensor-for-determination-of-colitoxin-dna-in-human-serum
#12
Qitong Huang, Xiaofeng Lin, Jie-Ji Zhu, Qing-Xiao Tong
In this study, a green and fast method was developed to synthesize high-yield carbon dots (CDs) via one-pot microwave treatment of banana peels without using any other surface passivation agents. Then the as-prepared CDs was used as the reducing agent and stabilizer to synthesize a Pd-Au@CDs nanocomposite by a simple sequential reduction strategy. Finally, Pd-Au@CDs nanocomposite modified glassy carbon electrode (Pd-Au@CDs/GCE) was obtained as a biosensor for target DNA after being immobilized a single-stranded probe DNA by a carboxyl ammonia condensation reaction...
March 22, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28343091/rapid-detection-and-ruling-out-of-neonatal-sepsis-by-pcr-coupled-with-electrospray-ionization-mass-spectrometry-pcr-esi-ms
#13
Cristina Delcò, Oliver Karam, Riccardo Pfister, Alain Gervaix, Gesuele Renzi, Stéphane Emonet, Jacques Schrenzel, Klara M Posfay-Barbe
BACKGROUND: Sepsis is an important cause of morbidity and mortality in neonates and clinicians are typically required to administer empiric antibiotics while waiting for blood culture results. However, prolonged and inappropriate use of antibiotics is associated with various complications and adverse events. Better tools to rapidly rule out bacterial infections are therefore needed. AIMS: We aimed to assess the negative predictive value of PCR coupled with Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) compared to conventional blood cultures in neonatal sepsis...
March 23, 2017: Early Human Development
https://www.readbyqxmd.com/read/28343061/automated-diabetic-macular-edema-dme-grading-system-using-dwt-dct-features-and-maculopathy-index
#14
U Rajendra Acharya, Muthu Rama Krishnan Mookiah, Joel E W Koh, Jen Hong Tan, Sulatha V Bhandary, A Krishna Rao, Yuki Hagiwara, Chua Kuang Chua, Augustinus Laude
The cause of diabetic macular edema (DME) is due to prolonged and uncontrolled diabetes mellitus (DM) which affects the vision of diabetic subjects. DME is graded based on the exudate location from the macula. It is clinically diagnosed using fundus images which is tedious and time-consuming. Regular eye screening and subsequent treatment may prevent the vision loss. Hence, in this work, a hybrid system based on Radon transform (RT), discrete wavelet transform (DWT) and discrete cosine transform (DCT) are proposed for an automated detection of DME...
March 19, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28343007/bioconcentration-and-multi-biomarkers-of-organic-uv-filters-bm-dbm-and-od-paba-in-crucian-carp
#15
Binni Ma, Guanghua Lu, Jianchao Liu, Zhenhua Yan, Haohan Yang, Ting Pan
Organic UV filters (OUV-Fs) are increasingly used in sunscreens and personal care products. In the present work, the bioconcentration and multi-biomarker effects of butyl methoxydibenzoylmethane (BM-DBM) and ethylhexyl dimethyl p-aminobenzoate (OD-PABA) were investigated in crucian carp (Carassius auratus). The fish were exposed to various concentrations of BM-DBM (3.88, 35.61, 181.85 and 337.15μg/L), OD-PABA (4.66, 53.83, 264.22 and 459.32μg/L) and their mixture (2.31+2.79, 23.69+26.18, 97.37+134.81 and 193...
March 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28342983/lipid-quantification-by-raman-microspectroscopy-as-a-potential-biomarker-in-prostate-cancer
#16
Jordan O'Malley, Rahul Kumar, Andrey Kuzmin, Artem Pliss, Neelu Yadav, Srimmitha Balachandar, Jianmin Wang, Kristopher Attwood, Paras N Prasad, Dhyan Chandra
Metastatic castration recurrent prostate cancer (mCRPC) remains incurable and is one of the leading causes of cancer-related death among American men. Therefore, detection of prostate cancer (PCa) at early stages may reduce PCa-related mortality in men. We show that lipid quantification by vibrational Raman Microspectroscopy and Biomolecular Component Analysis may serve as a potential biomarker in PCa. Transcript levels of lipogenic genes including sterol regulatory element-binding protein-1 (SREBP-1) and its downstream effector fatty acid synthase (FASN), and rate-limiting enzyme acetyl CoA carboxylase (ACACA) were upregulated corresponding to both Gleason score and pathologic T stage in the PRAD TCGA cohort...
March 23, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28342889/heteromeric-complexes-of-aldo-keto-reductase-auxiliary-kv%C3%AE-subunits-akr6a-regulate-sarcolemmal-localization-of-kv1-5-in-coronary-arterial-myocytes
#17
Matthew A Nystoriak, Deqing Zhang, Ganapathy Jagatheesan, Aruni Bhatnagar
Redox-sensitive potassium channels consisting of the voltage-gated K(+) (KV) channel pore subunit KV1.5 regulate resting membrane potential and thereby contractility of vascular smooth muscle cells. Members of the KV1 family associate with cytosolic auxiliary β subunits, which are members of the aldo-keto reductase (AKR) superfamily (AKR6A subfamily). The Kvβ subunits have been proposed to regulate Kv1 gating via pyridine nucleotide cofactor binding. However, the molecular identity of KVβ subunits that associate with native KV1...
March 22, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28342859/hydroethanolic-extract-from-echinodorus-scaber-rataj-leaves-inhibits-inflammation-in-ovalbumin-induced-allergic-asthma
#18
Suellen Iara Guirra Rosa, Fabrício Rios-Santos, Sikiru Olaitan Balogun, Danielle Ayr Tavares de Almeida, Amilcar Sabino Damazo, Thais Campos Dias da Cruz, Eduarda Pavan, Robson Dos Santos Barbosa, Tarso da Costa Alvim, Ilsamar Mendes Soares, Sérgio Donizeti Ascêncio, Antonio Macho, Domingos Tabajara de Oliveira Martins
ETHNOPHARMACOLOGICAL RELEVANCE: Echinodorus scaber, Alismataceae, is popularly known in Brazil as "chapéu-de-couro". The plant leaves are used by the population as decoction, infusion, or maceration in bottled spirits, to treat inflammatory respiratory diseases. AIM OF THE STUDY: To investigate the anti-inflammatory mechanism of the hydroethanolic extract of leaves of Echinodorus scaber (HEEs) in allergic asthma. A phytochemical analysis of the extract was performed as well...
March 22, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28342800/development-of-an-isothermal-recombinase-polymerase-amplification-assay-for-rapid-detection-of-pseudorabies-virus
#19
Yang Yang, Xiaodong Qin, Wei Zhang, Zhiyong Li, Shuaijun Zhang, Yanmin Li, Zhidong Zhang
Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA LFD assay was 100 copies per reaction and 160 copies per reaction, respectively. Both assays did not detect DNAs from other virus or PRV negative samples. Therefore, the developed RPA assays provide a rapid, simple, sensitive and specific alternative tool for detection of PRV...
March 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28342740/the-development-of-a-real-time-reverse-transcription-polymerase-chain-reaction-rrt-pcr-assay-using-taqman-technology-for-the-pan-detection-of-bluetongue-virus-btv
#20
Catherine Mulholland, Michael J McMenamy, Bernd Hoffmann, Bernadette Earley, Bryan Markey, Joseph Cassidy, Gordon Allan, Michael D Welsh, John McKillen
Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time. BTV-8 in particular, caused a major epidemic in northern Europe. As a result, it is evident that most European countries are at risk of BTV infection. The objective of this study was to develop and validate a real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) assay based on TaqMan technology for the detection of representative strains of all BTV serotypes...
March 22, 2017: Journal of Virological Methods
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