Craig G Rogers, Jonathon A Ditlev, Min-Han Tan, Jun Sugimura, Chao-Nan Qian, Jeff Cooper, Brian Lane, Michael A Jewett, Richard J Kahnoski, Eric J Kort, Bin T Teh
We investigate the feasibility of using microarray gene expression profiling technology to analyze core biopsies of renal tumors for classification of tumor histology. Core biopsies were obtained ex-vivo from 7 renal tumors-comprised of four histological subtypes-following radical nephrectomy using 18-gauge biopsy needles. RNA was isolated from these samples and, in the case of biopsy samples, amplified by in vitro transcription. Microarray analysis was then used to quantify the mRNA expression patterns in these samples relative to non-diseased renal tissue mRNA...
January 1, 2009: American Journal of Translational Research