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Cell-penetrating peptide

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https://www.readbyqxmd.com/read/29156626/surface-plasmon-resonance-sensing-of-biorecognition-interactions-within-the-tumor-suppressor-p53-network
#1
REVIEW
Ilaria Moscetti, Salvatore Cannistraro, Anna Rita Bizzarri
Surface Plasmon Resonance (SPR) is a powerful technique to study the kinetics of biomolecules undergoing biorecognition processes, particularly suited for protein-protein interactions of biomedical interest. The potentiality of SPR was exploited to sense the interactions occurring within the network of the tumor suppressor p53, which is crucial for maintaining genome integrity and whose function is inactivated, mainly by down regulation or by mutation, in the majority of human tumors. This study includes p53 down-regulators, p53 mutants and also the p53 family members, p63 and p73, which could vicariate p53 protective function...
November 20, 2017: Sensors
https://www.readbyqxmd.com/read/29155703/modeling-amyloid-%C3%AE-42-toxicity-and-neurodegeneration-in-adult-zebrafish-brain
#2
Prabesh Bhattarai, Alvin Kuriakose Thomas, Mehmet Ilyas Cosacak, Christos Papadimitriou, Violeta Mashkaryan, Yixin Zhang, Caghan Kizil
Alzheimer's disease (AD) is a debilitating neurodegenerative disease in which accumulation of toxic amyloid-β42 (Aβ42) peptides leads to synaptic degeneration, inflammation, neuronal death, and learning deficits. Humans cannot regenerate lost neurons in the case of AD in part due to impaired proliferative capacity of the neural stem/progenitor cells (NSPCs) and reduced neurogenesis. Therefore, efficient regenerative therapies should also enhance the proliferation and neurogenic capacity of NSPCs. Zebrafish (Danio rerio) is a regenerative organism, and we can learn the basic molecular programs with which we could design therapeutic approaches to tackle AD...
October 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155563/cellular-effects-and-delivery-propensity-of-penetratin-is-influenced-by-conjugation-to-parathyroid-hormone-fragment-1-34-in-synergy-with-ph
#3
Mie Kristensen, Line Hagner Nielsen, Kinga Zór, Anja Boisen, Malene Vinther Christensen, Jens Berthelsen, Hanne Mørck Nielsen
The cell-penetrating peptide (CPP) penetratin, has demonstrated potential as a carrier for transepithelial delivery of cargo peptides, such as the therapeutically relevant part of parathyroid hormone, i.e. PTH(1-34). The purpose of the present study was to elucidate the relevance of modifying the pH for PTH(1-34)-penetratin conjugates and for co-administered penetratin with PTH(1-34) in terms of transepithelial permeation of PTH(1-34) and cellular effects. Transepithelial permeation was assessed using monolayers of the Caco-2 cell culture model, and effects on Caco-2 cellular viability kinetics were evaluated by using the Real-Time-GLO assay as well as by microscopy following Tryphan blue staining...
November 20, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29152602/shortened-penetratin-cell-penetrating-peptide-is-insufficient-for-cytosolic-delivery-of-a-grb7-targeting-peptide
#4
Gabrielle M Watson, Ketav Kulkarni, Rebecca Brandt, Mark P Del Borgo, Marie-Isabel Aguilar, Jacqueline A Wilce
Delivery across the cell membrane is of critical importance for the development of therapeutics targeting intracellular proteins. The use of cell-penetrating peptides (CPPs), such as Penetratin (P16), has facilitated the delivery of otherwise cell-impermeable molecules allowing them to carry out their biological function. A truncated form of Penetratin (RRMKWKK) has been previously described as the minimal Penetratin sequence that is required for translocation across the cell membrane. Here, we performed a detailed comparison of cellular uptake by Penetratin (P16) and the truncated Penetratin peptide (P7), including their ability to deliver G7-18NATE, a cyclic peptide targeting the cytosolic cancer target Grb7-adapter protein into cells...
February 28, 2017: ACS Omega
https://www.readbyqxmd.com/read/29150077/unique-arginine-array-improves-cytosolic-localization-of-hydrocarbon-stapled-peptides
#5
Kim Quach, Jonathan LaRochelle, Xiao-Han Li, Elizabeth Rhoades, Alanna Schepartz
We have previously reported that miniature proteins containing a distinct array of 5 arginine residues on a folded α-helix - a penta-arg motif - traffic with high efficiency from endosomes into the cytosol and nucleus of mammalian cells. Here we evaluate whether a penta-arg motif can improve the intracellular trafficking of an otherwise impermeant hydrocarbon-stapled peptide, SAH-p53-4(Rho). We prepared a panel of SAH-p53-4(Rho) variants containing penta-arg sequences with different spacings and axial arrangement and evaluated their overall uptake (as judged by flow cytometry) and their intracellular access (as determined by fluorescence correlation spectroscopy, FCS)...
November 7, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29149694/a-high-brightness-probe-of-polymer-nanoparticles-for-biological-imaging
#6
Sirong Zhou, Jiarong Zhu, Yaping Li, Liheng Feng
Conjugated polymer nanoparticles (CPNs) with high brightness in long wavelength region were prepared by the nano-precipitation method. Based on fluorescence resonance energy transfer (FRET) mechanism, the high brightness property of the CPNs was realized by four different emission polymers. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) displayed that the CPNs possessed a spherical structure and an average diameter of ~75nm. Analysis assays showed that the CPNs had excellent biocompatibility, good photostability and low cytotoxicity...
November 11, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29143522/mmp-2-sensitive-ha-end-conjugated-poly-amidoamine-dendrimers-via-click-reaction-to-enhance-drug-penetration-into-solid-tumor
#7
Min Han, Ming-Yi Huang-Fu, Wang-Wei Guo, Ningning Guo, Jiejian Chen, Huina Liu, Zhiqi Xie, Mengting Lin, Qichun Wei, Jianqing Gao
Currently, the limited tumor penetration of nanoparticles remains a major challenge of cancer nanomedicine. Herein, we propose a size-shrinkable, drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29136833/a-cell-penetrating-ratiometric-probe-for-simultaneous-measurement-of-lysosomal-and-cytosolic-ph-change
#8
Meng-Chan Xia, Lesi Cai, Sichun Zhang, Xinrong Zhang
A new ratiometric fluorescent probe based on cell-penetrating peptides (CPPs) was constructed for whole-cell pH mapping and simultaneous measurement of pH changes in the cytoplasm and lysosomes. The arginine-rich CPP, R12K worked as linker, carrier and part of the fluorophore. Benefiting from R12K, the fluorescent probe is completely water soluble, membrane permeable and well biocompatible. It shows high selectivity, sensitivity and reversibility to pH fluctuations. The ratio of fluorescence intensities F519/F582 increased from 0...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29133235/can-the-cellular-internalization-of-cargo-proteins-be-enhanced-by-fusing-a-tat-peptide-in-the-center-of-proteins-a-fluorescence-study
#9
Xiaochao Chen, Jing Chen, Rong Fu, Pingfan Rao, Richard Weller, Jeremy Brasdshaw, Shutao Liu
Aim to investigate whether the cellular uptake of cargo proteins can be enhanced by fusing a Tat peptide in the center of proteins, GST-Tat-GFP and GST-GFP-Tat proteins were firstly constructed and expressed. The cellular internalization of both proteins was then evaluated and compared in HeLa cells by using fluorescent microscopy and flow cytometry, as well as the transdermal delivery in human skin by using confocal microscopy. Results from in-vitro cell experiments showed that GST-Tat-GFP protein efficiently internalized into HeLa cells when a Tat peptide was fused in the center of proteins, whereas its efficiency is lower than that of GST-GFP-Tat protein with a Tat peptide terminal fused...
November 10, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29133119/towards-topical-microrna-directed-therapy-for-epidermal-disorders
#10
REVIEW
Kehinde Ross
There remains an unmet need dermatological need for innovative topical agents that achieve better long term outcomes with fewer side effects. Modulation of the expression and activity of microRNA (miRNAs) represents an emerging translational framework for the development of such innovative therapies because changes in the expression of one miRNA can have wide-ranging effects on diverse cellular processes associated with disease. In this short review, the roles of miRNA in epidermal development, psoriasis, cutaneous squamous cell carcinoma and re-epithelisation are highlighted...
November 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29125836/a-simple-in-vitro-tumor-chemosensitivity-assay-based-on-cell-penetrating-peptide-tagged-luciferase
#11
Tingyu Yu, Jiao Lin, Jin Zhao, Wei Huang, Lingwen Zeng, Zhiyuan Fang, Ning Xu
The analysis of intracellular ATP can reveal the response of cells to different treatments and is important for individualized medicine. In the present study, we developed a cell penetrating peptides (CPPs) tagged luciferase (TAT-LUC) for tumor chemosensitivity assay. The activity of recombinant TAT-LUC was evaluated using ATP standard solution and tumor cells. This recombinant TAT-LUC was then used for the analysis of sensitivity index (SI) of four strains of tumor cells. The results showed that TAT-LUC could detect less than 10 nM extracellular ATP with a strong correlation between the luminescence intensity and the ATP content (R2 = 0...
2017: PloS One
https://www.readbyqxmd.com/read/29125066/autonomous-self-navigating-drug-delivery-vehicles-from-science-fiction-to-reality
#12
Valery A Petrenko
Low efficacy of targeted nanomedicines in biological experiments enforced us to challenge the traditional concept of drug targeting and suggest a paradigm of 'addressed self-navigating drug-delivery vehicles,' in which affinity selection of targeting peptides and vasculature-directed in vivo phage screening is replaced by the migration selection, which explores ability of 'promiscuous' phages and their proteins to migrate through the tumor-surrounding cellular barriers, using a 'hub and spoke' delivery strategy, and penetrate into the tumor affecting the diverse tumor cell population...
December 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/29123382/in-vivo-study-of-doxorubicin-loaded-cell-penetrating-peptide-modified-ph-sensitive-liposomes-biocompatibility-bio-distribution-and-pharmacodynamics-in-balb-c-nude-mice-bearing-human-breast-tumors
#13
Yuan Ding, Wei Cui, Dan Sun, Gui-Ling Wang, Yu Hei, Shuai Meng, Jian-Hua Chen, Ying Xie, Zhi-Qiang Wang
In vivo evaluation of drug delivery vectors is essential for clinical translation. In BALB/c nude mice bearing human breast cancer tumors, we investigated the biocompatibility, pharmacokinetics, and pharmacodynamics of doxorubicin (DOX)-loaded novel cell-penetrating peptide (CPP)-modified pH-sensitive liposomes (CPPL) (referred to as CPPL(DOX)) with an optimal CPP density of 4%. In CPPL, a polyethylene glycol (PEG) derivative formed by conjugating PEG with stearate via acid-degradable hydrazone bond (PEG2000-Hz-stearate) was inserted into the surface of liposomes, and CPP was directly attached to liposome surfaces via coupling with stearate to simultaneously achieve long circulation time in blood and improve the selectivity and efficacy of CPP for tumor targeting...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29117144/cell-penetrating-peptides-design-strategies-beyond-primary-structure-and-amphipathicity
#14
REVIEW
Daniela Kalafatovic, Ernest Giralt
Efficient intracellular drug delivery and target specificity are often hampered by the presence of biological barriers. Thus, compounds that efficiently cross cell membranes are the key to improving the therapeutic value and on-target specificity of non-permeable drugs. The discovery of cell-penetrating peptides (CPPs) and the early design approaches through mimicking the natural penetration domains used by viruses have led to greater efficiency of intracellular delivery. Following these nature-inspired examples, a number of rationally designed CPPs has been developed...
November 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29115187/hyaluronate-parathyroid-hormone-peptide-conjugate-for-transdermal-treatment-of-osteoporosis
#15
Minsoo Cho, Seulgi Han, Hyemin Kim, Ki Su Kim, Sei Kwang Hahn
Human parathyroid hormone 1-34 fragment (PTH1-34) has been used as a FDA-approved therapeutics to treat osteoporosis by daily subcutaneous injection. In this work, we successfully developed PTH1-34 conjugated hyaluronic acid (HA) for the transdermal treatment of osteoporosis with improved patient compliance. HA-PTH1-34 conjugate was synthesized by the coupling reaction between aldehyde group introduced to HA and amine group of PTH1-34. After characterization by gel permeation chromatography (GPC) and ELISA, the biological effect of HA-PTH1-34 conjugate on the proliferation of human osteoblast cells was confirmed by in vitro calcium colorimetric assay and cAMP assay...
November 8, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29113134/non-covalent-loading-of-anti-cancer-doxorubicin-by-modularizable-peptide-self-assemblies-for-a-nanoscale-drug-carrier
#16
Kin-Ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui
We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining β-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations...
November 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29109776/sequential-delivery-of-cyclopeptide-ra-v-and-doxorubicin-for-combination-therapy-on-resistant-tumor-and-in-situ-monitoring-of-cytochrome-c-release
#17
Huachao Chen, Yurong Wang, Yongrong Yao, Shenglin Qiao, Hao Wang, Ninghua Tan
A programmed drug delivery system that can achieve sequential release of multiple therapeutics under different stimulus holds great promise to enhance the treatment efficacy and overcome multi-drug resistance (MDR) in tumor. Herein, multi-organelle-targeted and pH/ cytochrome c (Cyt c) dual-responsive nanoparticles were designed for combination therapy on resistant tumor. In this system (designated DGLipo NPs), doxorubicin (Dox) was intercalated into the DNA duplex containing a Cyt c aptamer, which subsequently loaded in the dendrigraftpoly-L-lysines (DGL) cores of DGLipo NPs, while cyclopeptide RA-V was doped into the pH-sensitive liposomal shells...
2017: Theranostics
https://www.readbyqxmd.com/read/29108892/antifungal-activity-of-latarcin-1-derived-cell-penetrating-peptides-against-fusarium-solani
#18
Deepthi Poornima Budagavi, Sheeba Zarin, Archana Chugh
Cell-penetrating peptides and antimicrobial peptides share physicochemical characteristics and mechanisms of interaction with biological membranes, hence, termed as membrane active peptides. The present study aims at evaluating AMP activity of CPPs. LDP-NLS and LDP are Latarcin 1 derived cell-penetrating peptides and in the current study we have evaluated antifungal and cell-penetrating properties of these CPPs in Fusarium solani. We observed that LDP-NLS and LDP exhibited excellent antifungal activity against the fungus...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29100129/liposomes-containing-cholesterol-and-mitochondria-penetrating-peptide-mpp-for-targeted-delivery-of-antimycin-a-to-a549-cells
#19
Sudipta Mallick, Le Thi Thuy, Seulgi Lee, Jong-Ii Park, Joon Sig Choi
No abstract text is available yet for this article.
October 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29095595/matrix-metalloproteinase-cleavable-nanoparticles-for-tumor-microenvironment-and-tumor-cell-dual-targeting-drug-delivery
#20
Zhenliang Sun, Ruihong Li, Ji Sun, You Peng, Linlin Xiao, Xingxing Zhang, Yixin Xu, Man Wang
Matrix metalloproteinases (MMPs), mostly abundant in the tumor extracellular matrix (ECM), tumor cells, and tumor vasculatures, are closely correlated with tumor progression and metastasis. In this case, making use of MMPs was supposed to achieve site-specific drug delivery and a satisfactory tumor treatment effect. Herein, we rationally developed a novel tumor microenvironment and tumor cell dual-targeting nanoparticle by integrating a chemotherapeutic-loaded drug-loaded carrier and a versatile polypeptide-LinTT1-PVGLIG-TAT (LPT) which is composed of a multitargeting peptide-LinTT1 and a cell-penetrating peptide-TAT...
November 9, 2017: ACS Applied Materials & Interfaces
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