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Cell-penetrating peptide

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https://www.readbyqxmd.com/read/27924991/improving-cell-penetration-of-helical-peptides-stabilized-by-n-terminal-crosslinked-aspartic-acids
#1
Hui Zhao, Yanhong Jiang, Yuan Tian, Dan Yang, Xuan Qin, Zigang Li
Cell penetration and nucleus translocation efficiency are important for the cellular activities of peptide therapeutics. For helical peptides stabilized by N-terminal crosslinked aspartic acid, correlations between their penetration efficiency/nucleus translocation and physicochemical properties were studied. An increase in hydrophobicity and isoelectric point will promote cellular uptake and nucleus translocation of stabilized helices.
December 7, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27923812/an-l-to-d-amino-acid-conversion-in-the-cell-penetrating-peptide-dftat-influences-proteolytic-stability-endocytic-uptake-and-endosomal-escape
#2
Kristina Najjar, Alfredo Erazo-Oliveras, Dakota J Brock, Ting-Yi Wang, Jean-Philippe Pellois
Cell-penetrating peptides (CPPs) are well established as delivery agents for otherwise cell-impermeable cargos. CPPs can also theoretically be used to modulate intracellular processes. However, their susceptibility to proteolytic degradation often limits their utility in these applications. Previous studies have explored the consequences on cellular uptake of converting the residues in CPPs from L to D stereochemistry, but conflicting results have been reported and specific steps en route to intracellular activity have not been explored...
December 6, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27921280/ph-responsive-triblock-copolymeric-micelles-decorated-with-a-cell-penetrating-peptide-provide-efficient-doxorubicin-delivery
#3
Khen Eng Ng, Mohd Cairul Iqbal Mohd Amin, Haliza Katas, Muhammad Wahab Amjad, Adeel Masood Butt, Prashant Kesharwani, Arun K Iyer
This study developed novel triblock pH-responsive polymeric micelles (PMs) using cholic acid-polyethyleneimine-poly-L-arginine (CA-PEI-pArg) copolymers. PEI provided pH sensitivity, while the hydrophilic cell-penetrating pArg peptide promoted cellular PM internalization. The copolymers self-assembled into PMs in aqueous solution at above the critical micelle concentration (2.98 × 10(-7) M) and encapsulated doxorubicin in the core region, with a 34.2% (w/w) entrapment efficiency. PMs showed pH-dependent swelling, increasing in size by almost sevenfold from pH 7...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27920533/effect-of-inserted-spacer-in-hepatic-cell-penetrating-multifunctional-peptide-component-on-the-dna-intracellular-delivery-of-quaternary-complexes-based-on-modular-design
#4
Luchen Zhang, Zhenbo Li, Fangli Sun, Yuhong Xu, Zixiu Du
A safe and efficient quaternary gene delivery system (named Q-complexes) was constructed based on self-assembly of molecules through noncovalent bonds. This system was formulated through the cooperation and competing interactions of cationic liposomes, multifunctional peptides, and DNA, followed by coating hyaluronic acid on the surface of the ternary complexes. The multifunctional peptide was composed of two functional domains: penetrating hepatic tumor-targeted cell moiety (KRPTMRFRYTWNPMK) and a wrapping gene sequence (polyarginine 16)...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27917413/efficacy-of-s-lacosamide-in-preclinical-models-of-cephalic-pain
#5
Aubin Moutal, Nathan Eyde, Edwin Telemi, Ki Duk Park, Jennifer Y Xie, David W Dodick, Frank Porreca, Rajesh Khanna
Migraine is one of the world's most common neurological disorders. Current acute migraine treatments have sub-optimal efficacy and new therapeutic options are needed. Approaches targeting calcitonin gene related peptide (CGRP) signaling are clinically effective but small molecule antagonists have not been advanced due to toxicity. In this study, we explored the axonal growth/specification collapsin response mediator protein 2 (CRMP2) as a novel "druggable" target for inhibiting CGRP release and for potential relevance for treatment of migraine pain...
June 2016: Pain Reports (Baltimore, Md.)
https://www.readbyqxmd.com/read/27916828/enhancing-anticancer-effect-of-gefitinib-across-the-blood-brain-barrier-model-using-liposomes-modified-with-one-%C3%AE-helical-cell-penetrating-peptide-or-glutathione-and-tween-80
#6
Kuan-Hung Lin, Shu-Ting Hong, Hsiang-Tsui Wang, Yu-Li Lo, Anya Maan-Yuh Lin, James Chih-Hsin Yang
Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI), such as gefitinib, have been demonstrated to effectively treat the patients of extracranial non-small cell lung cancer (NSCLC). However, these patients often develop brain metastasis (BM) during their disease course. The major obstacle to treat BM is the limited penetration of anticancer drugs across the blood-brain barrier (BBB). In the present study, we utilized gefitinib-loaded liposomes with different modifications to improve gefitinib delivery across the in vitro BBB model of bEnd...
November 29, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27908581/sonodynamic-inactivation-of-gram-positive-and-gram-negative-bacteria-using-a-rose-bengal-antimicrobial-peptide-conjugate
#7
David Costley, Heather Nesbitt, Nigel Ternan, James Dooley, Ying-Ying Huang, Michael R Hamblin, Anthony P McHale, John F Callan
Combating antimicrobial resistance is one of the most serious public health challenges facing society today. The development of new antibiotics or alternative techniques that can help combat antimicrobial resistance is being prioritised by many governments and stakeholders across the globe. Antimicrobial photodynamic therapy is one such technique that has received considerable attention but is limited by the inability of light to penetrate through human tissue, reducing its effectiveness when used to treat deep-seated infections...
November 17, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27902468/synthesis-of-a-cell-penetrating-peptide-modified-superparamagnetic-iron-oxide-and-mri-detection-of-bladder-cancer
#8
Chen Ding, Kaijie Wu, Weiyi Wang, Zhenfeng Guan, Lei Wang, Xinyang Wang, Rong Wang, Li Liu, Jinhai Fan
Bladder cancer is the most common malignancy of the urinary tract for which the accurate measurement of minimal residual disease is critical to treatment and determining prognosis. Although cystoscope examination and voided urine cytology remain the current standard of care for detecting residual disease, these approaches are limited by mechanical trauma and lack sensitivity. To develop a new accurate noninvasive method, we developed a novel contrast agent where the surface of superparamagnetic iron oxide (SPIO) nanoparticles is functionalized with a bladder cancer-specific fluorescein isothiocyanate (FITC) labeled cell penetrating peptide (CPP)-polyarginine peptides (R11) for active targeting and imaging...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27900805/effect-of-preorganized-charge-display-on-the-cell-penetrating-properties-of-cationic-peptides
#9
Yvonne A Nagel, Philipp S Raschle, Helma Wennemers
The effect of preorganized versus undefined charge display on the cellular uptake of cationic cell-penetrating peptides (CPPs) was investigated by comparing conformationally well-defined guanidinylated oligoprolines with flexible oligoarginines. Flow cytometry and confocal microscopy studies with different cancer cell lines (HeLa, MCF-7, and HT-29) showed that preorganization of cationic charges in lateral distances of ≈9 Å enhanced the cellular uptake of CPPs. Binding affinity measurements revealed tighter binding of analogues of cell-surface glycans to the guanidinylated octaproline with localized charges compared to flexible octaarginine, a finding that was further correlated to the cellular uptake by studies with CHO cells deficient in glycans on the outer plasma membrane...
November 30, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27899989/tumor-penetration-and-antitumor-efficacy-of-cetuximab-are-enhanced-by-co-administered-irgd-in-a-murine-model-of-human-nsclc
#10
Yang Zhang, Jie Yang, Manhua Ding, Liantao Li, Zheng Lu, Qing Zhang, Junnian Zheng
Lung cancer is the leading cause of cancer-associated mortality, worldwide. For this reason, novel therapies are required for the treatment of this devastating disease. Cetuximab is a monoclonal antibody against epidermal growth factor receptor (EGFR), which is overexpressed in a variety of solid tumors, including non-small cell lung cancer (NSCLC). The therapeutic efficacy of cetuximab for NSCLC is limited to use as a monotherapy or in combination with chemotherapy. The objective of the present study was to develop a novel strategy to enhance the therapeutic efficacy of cetuximab for NSCLC by a co-administration with the tumor-penetrating internalizing RGD peptide (iRGD)...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27899251/improved-breast-cancer-cell-specific-intracellular-drug-delivery-and-therapeutic-efficacy-by-coupling-decoration-with-cell-penetrating-peptide-and-sp90-peptide
#11
Li-Qiang Fan, Guo-Xiu Du, Peng-Fei Li, Ming-Wei Li, Yao Sun, Li-Ming Zhao
Lack of satisfactory specificity towards tumor cells and poor intracellular delivery efficacy are the major drawbacks with conventional cancer chemotherapy. Conjugated anticancer drugs to targeting moieties e.g. to peptides with the ability to recognize cancer cells and to cell penetrating peptide can improve these characteristics, respectively. Combining a tumor homing peptide with an appropriate cell-penetrating peptide can enhance the tumor-selective internalization efficacy of the carrying cargo molecules...
November 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27895484/the-effect-of-dexamethasone-cell-penetrating-peptide-nanoparticles-on-gene-delivery-for-inner-ear-therapy
#12
Ji Young Yoon, Keum-Jin Yang, Shi-Nae Park, Dong-Kee Kim, Jong-Duk Kim
Dexamethasone (Dex)-loaded PHEA-g-C18-Arg8 (PCA) nanoparticles (PCA/Dex) were developed for the delivery of genes to determine the synergistic effect of Dex on gene expression. The cationic PCA nanoparticles were self-assembled to create cationic micelles containing an octadecylamine (C18) core with Dex and an arginine 8 (Arg8) peptide shell for electrostatic complexation with nucleic acids (connexin 26 [Cx26] siRNA, green fluorescent protein [GFP] DNA or brain-derived neurotrophic factor [BDNF] pDNA). The PCA/Dex nanoparticles conjugated with Arg8, a cell-penetrating peptide that enhances permeability through a round window membrane in the inner ear for gene delivery, exhibited high uptake efficiency in HEI-OC1 cells...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27894924/antidepressant-like-effects-exerted-by-the-intranasal-administration-of-a-glucagon-like-peptide-2-derivative-containing-cell-penetrating-peptides-and-a-penetration-accelerating-sequence-in-mice
#13
Sachie Sasaki-Hamada, Ryuji Nakamura, Yusuke Nakao, Toshiki Akimoto, Emi Sanai, Mio Nagai, Michiko Horiguchi, Chikamasa Yamashita, Jun-Ichiro Oka
The intracerebroventicular (i.c.v.) administration of glucagon-like peptide-2 (GLP-2) to rodents was shown to have antidepressant-like effects in imipramine-resistant depression-model mice. In order to utilize GLP-2 as a clinical treatment tool for depression, we herein focused on the intranasal delivery that is non-invasive approach, because the i.c.v. administration is invasive and impractical. In the present study, we prepared a GLP-2 derivative containing cell penetrating peptides (CPPs) and a penetration accelerating sequence (PAS) (PAS-CPPs-GLP-2) for the intranasal (i...
November 25, 2016: Peptides
https://www.readbyqxmd.com/read/27893441/engineering-tumor-cell-targeting-in-nanoscale-amyloidal-materials
#14
Ugutz Unzueta, Joaquin Seras-Franzoso, María Virtudes Céspedes, Paolo Saccardo, Francisco Cortés, Fabián Rueda, Elena Garcia-Fruitós, Neus Ferrer-Miralles, Ramon Mangues, Esther Vázquez, Antonio Villaverde
Bacterial inclusion bodies are non-toxic, mechanically stable and functional protein amyloids within the nanoscale size range that are able to naturally penetrate into mammalian cells, where they deliver the embedded protein in a functional form. The potential use of inclusion bodies in protein delivery or protein replacement therapies is strongly impaired by the absence of specificity in cell binding and penetration, thus preventing targeting. To address this issue, we have here explored whether the genetic fusion of two tumor-homing peptides, the CXCR4 ligands R9 and T22, to an inclusion body-forming green fluorescent protein (GFP), would keep the interaction potential and the functionality of the fused peptides and then confer CXCR4 specificity in cell binding and further uptake of the materials...
January 6, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27890856/sirna-cell-penetrating-peptides-complexes-as-a-combinatorial-therapy-against-chronic-myeloid-leukemia-using-bv173-cell-line-as-model
#15
João Miguel Freire, Inês Rego de Figueiredo, Javier Valle, Ana Salomé Veiga, David Andreu, Francisco J Enguita, Miguel A R B Castanho
Chronic myeloid leukemia (CML) is a myeloproliferative disorder caused by a single gene mutation, a reciprocal translocation that originates the Bcr-Abl gene with constitutive tyrosine kinase activity. As a monogenic disease, it is an optimum target for RNA silencing therapy. We developed a siRNA-based therapeutic approach in which the siRNA is delivered by pepM or pepR, two cell-penetrating peptides (CPPs) derived from the dengue virus capsid protein. These peptides have a dual role: siRNA delivery into cells and direct action as bioportides, i...
November 24, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27889841/the-function-of-activatable-cell-penetrating-peptides-in-human-intrahepatic-bile-duct-epithelial-cells
#16
Kui Tu, Lijin Zhao, Jin Gu, Pengzhan Yan, Fang Wang, Yu Cao
This study aimed to investigate the function of Activatable Cell-Penetrating Peptides (ACPP) in detecting the changes of human intrahepatic bile duct epithelial cell(hIBDEC). ACPP, which target matrix metalloproteinases, were constructed. All were labeled with FITC and Gd-DTPA at the N-terminal. Fluorescence microscopy was used to observe the fluorescence intensity inside hIBDEC after stimulating with different concentrations of LPS and incubating with different concentrations of ACPP to determine the optimal concentration range for LPS stimulation and the optimal concentration for FITC-ACPP effect...
November 26, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27887988/in-vitro-and-in-vivo-delivery-of-therapeutic-proteins-using-cell-penetrating-peptides
#17
REVIEW
Azam Bolhassani, Behnaz Sadat Jafarzade, Golnaz Mardani
The failure of proteins to penetrate mammalian cells or target tumor cells restricts their value as therapeutic tools in a variety of diseases such as cancers. Recently, protein transduction domains (PTDs) or cell penetrating peptides (CPPs) have been shown to promote the delivery of therapeutic proteins or peptides into live cells. The successful delivery of proteins mainly depends on their physicochemical properties. Although, linear cell penetrating peptides are one of the most effective delivery vehicles; but currently, cyclic CPPs has been developed to potently transport bioactive full-length proteins into cells...
November 22, 2016: Peptides
https://www.readbyqxmd.com/read/27886311/flexible-synthesis-of-cationic-peptide-porphyrin-derivatives-for-light-triggered-drug-delivery-and-photodynamic-therapy
#18
R Dondi, E Yaghini, K M Tewari, L Wang, F Giuntini, M Loizidou, A J MacRobert, I M Eggleston
Efficient syntheses of cell-penetrating peptide-porphyrin conjugates are described using a variety of bioconjugation chemistries. This provides a flexible means to convert essentially hydrophobic tetrapyrolle photosensitisers into amphiphilic derivatives which are well-suited for use in light-triggered drug delivery by photochemical internalisation (PCI) and targeted photodynamic therapy (PDT).
November 25, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27885926/short-polyhistidine-peptides-penetrate-effectively-into-nicotiana-tabacum-cultured-cells-and-saccharomyces-cerevisiae-cells
#19
Sayaka Kimura, Tsuyoshi Kawano, Takashi Iwasaki
The polyhistidine peptides (PHPs) have been previously reported as novel cell-penetrating peptides and are efficiently internalized into mammal cells; however, penetration of PHPs into other cell types is unknown. In this study, the cellular uptake of PHPs in plant and yeast cells was found to be dependent on the number of histidines, and short PHPs (H6-H10 peptides) showed effective internalization. The H8 peptide showed the highest cell-penetrating capacity and localized to vacuoles in plant and yeast cells...
January 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27881484/cell-penetrating-peptides-recruit-type-a-scavenger-receptors-to-the-plasma-membrane-for-cellular-delivery-of-nucleic-acids
#20
Carmen Juks, Annely Lorents, Piret Arukuusk, Ülo Langel, Margus Pooga
Scavenger receptors (SRs) are a large family of multifunctional receptors that are involved in a range of physiologic and pathologic processes. The ability of class A scavenger receptors (SRs)-As to bind anionic ligands facilitates the internalization of negatively charged cell-penetrating peptide (CPP)-nucleic acid nanocomplexes and thus makes them attractive targets for delivery of various nucleic acids. Recently, we demonstrated that SR-A3 and SR-A5 are recruited from intracellular membranes to the plasma membrane after incubation with PepFect 14-splice-switching oligonucleotide complexes...
November 23, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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