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Infectious bursal disease virus

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https://www.readbyqxmd.com/read/29235573/constitutively-elevated-levels-of-socs1-suppress-innate-responses-in-df-1-immortalised-chicken-fibroblast-cells
#1
E S Giotis, C S Ross, R C Robey, A Nohturfft, S Goodbourn, M A Skinner
The spontaneously immortalised DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEFs) for studies on avian viruses such as avian influenza but no comprehensive study has as yet been reported comparing their innate immunity phenotypes. We conducted microarray analyses of DF-1 and CEFs, under both normal and stimulated conditions using chicken interferon-α (chIFN-α) and the attenuated infectious bursal disease virus vaccine strain PBG98. We found that DF-1 have an attenuated innate response compared to CEFs...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29230245/adaptation-and-molecular-characterization-of-two-malaysian-very-virulent-infectious-bursal-disease-virus-isolates-adapted-in-bgm-70-cell-line
#2
Nafi'u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris
Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route. The EP12 isolate was further adapted and serially propagated in BGM-70 cell line up to 20 passages (P20). Characteristic cytopathic effects (CPEs) were subtly observed at P1 in both isolates 72 hours postinoculation (pi)...
2017: Advances in Virology
https://www.readbyqxmd.com/read/29217325/protocatechuic-acid-pca-induced-a-better-antiviral-effect-by-immune-enhancement-in-spf-chickens
#3
Yongxia Guo, Qiang Zhang, Zonghui Zuo, Jun Chu, Hongzhi Xiao, M Tariq Javed, Cheng He
Protocatechuic acid (PCA) is an antiviral agent against Avian Influenza virus (AIV) and Infectious Bursal Disease (IBD) virus but its antiviral mechanism is unknown. In this study, we evaluated the humoral and cellular responses to PCA in specific pathogen-free (SPF) chickens. One hundred forty 35-day-old SPF chickens were randomly divided into 7 groups. The birds were inoculated with the commercial, attenuated Newcastle Disease Virus (NDV) vaccine and then received orally with 10, 20 or 40 mg/kg body weight of PCA for 21 days...
December 4, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29211914/stinging-nettle-and-neem-enhance-antibody-response-to-local-killed-and-imported-live-infectious-bursal-disease-vaccines-in-indigenous-chicken-in-kenya
#4
M O Bwana, L W Njagi, P N Nyaga, P G Mbuthia, L C Bebora, M W Wahome, W U Mutinda, P M Kitala
Immune responses are critical for protection of chickens from infectious bursal disease (IBD). In this study, the antibody response-enhancing effect of drinking water supplementation of 1% stinging nettle and neem on different IBD vaccines and vaccination regimes was evaluated, using 36 (n = 36) specific antibody negative indigenous chicks. The birds were allocated into 3 groups as follows: 1A-C, 2A-C, and 3A-B, while group 3C acted as the unvaccinated non-supplemented control. A local inactivated K1 and imported live attenuated D78 IBD vaccines were given to groups 1A-C and 3A-B at 14 and 28 d of age, respectively...
December 2, 2017: Poultry Science
https://www.readbyqxmd.com/read/29184548/fused-igy-fc-and-polysaccharide-adjuvant-enhanced-the-immune-effect-of-the-recombinant-vp2-and-vp5-subunits-a-prospect-for-improvement-of-infectious-bursal-disease-virus-subunit-vaccine
#5
Huining Wang, Sufeng Shan, Shujuan Wang, Hao Zhang, Lili Ma, Liping Hu, He Huang, Kai Wei, Ruiliang Zhu
Infectious bursal disease virus (IBDV) is a highly contagious pathogen that causes damage in lymphoid organs and remains a threat to the poultry industry worldwide. Currently, subunit vaccines based on VP2 antigen expressed in prokaryotic systems are widely used in clinical settings. However, the immunogenicity of VP2 vaccines is limited because of their inherent defect that the structure of the antigen expressed in Escherichia coli (E. coli) may be different from its natural conformation. In this study, we fused VP2 and VP5 protective antigen genes and linked the chicken IgY Fc gene onto it...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29169149/prokaryotic-expression-and-anti-ibdv-activity-of-chicken-interleukin-18-and-interferon-%C3%AE
#6
Baifen Song, Xiaoting Li, Jinzhu Ma, Liquan Yu, Zhenyue Feng, Zhenhua Liu, Yudong Cui
Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified...
November 24, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29154745/differential-gene-expression-in-chicken-primary-b-cells-infected-ex-vivo-with-attenuated-and-very-virulent-strains-of-infectious-bursal-disease-virus-ibdv
#7
Katherine L Dulwich, Efstathios S Giotis, Alice Gray, Venugopal Nair, Michael A Skinner, Andrew J Broadbent
Infectious bursal disease virus (IBDV) belongs to the family Birnaviridae and is economically important to the poultry industry worldwide. IBDV infects B cells in the bursa of Fabricius (BF), causing immunosuppression and morbidity in young chickens. In addition to strains that cause classical Gumboro disease, the so-called 'very virulent' (vv) strain, also in circulation, causes more severe disease and increased mortality. IBDV has traditionally been controlled through the use of live attenuated vaccines, with attenuation resulting from serial passage in non-lymphoid cells...
November 20, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29146236/infectious-bursal-disease-virus-protein-vp4-suppresses-type-i-interferon-expression-via-inhibiting-k48-linked-ubiquitylation-of-glucocorticoid-induced-leucine-zipper-gilz
#8
Zhiyuan He, Xiang Chen, Mengjiao Fu, Jun Tang, Xiaoqi Li, Hong Cao, Yongqiang Wang, Shijun J Zheng
Viruses have developed a variety of methods to evade host immune response. Our previous study showed that infectious bursal disease virus (IBDV) inhibited type I interferon production via interaction of VP4 with cellular glucocorticoid-induced leucine zipper (GILZ) protein. However, the exact underlying molecular mechanism is still unclear. In this study, we found that IBDV VP4 suppressed GILZ degradation by inhibiting K48-linked ubiquitylation of GILZ. Furthermore, mutation of VP4 (R41G) abolished the inhibitory effect of VP4 on IFN-β expression and GILZ ubiquitylation, indicating that the amino acid 41R of VP4 was required for the suppression of IFN-β expression and GILZ ubiquitylation...
October 28, 2017: Immunobiology
https://www.readbyqxmd.com/read/29093101/vp1-and-vp3-are-required-and-sufficient-for-translation-initiation-of-uncapped-ibdv-genomic-dsrna
#9
Chengjin Ye, Yu Wang, Enli Zhang, Xinpeng Han, Zhaoli Yu, Hebin Liu
Infectious bursal disease virus (IBDV) is a bi-segmented double-strand RNA (dsRNA) virus of the Birnaviridae family. While IBDV genomic dsRNA lacks a 5' cap, the means by which the uncapped IBDV genomic RNA is translated effectively is unknown. In this study, we describe a cap-independent pathway of translation initiation of IBDV uncapped RNA that relies on VP1 and VP3. We show that neither purified IBDV genomic dsRNA nor the uncapped viral plus-sense RNA transcripts was directly translated and rescued into infectious viruses in host cells...
November 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29083994/assembly-disassembly-of-a-complex-icosahedral-virus-to-incorporate-heterologous-nucleic-acids
#10
Elena Pascual, Carlos P Mata, José L Carrascosa, Jose R Caston
Hollow protein containers are widespread in nature, and include virus capsids as well as eukaryotic and bacterial complexes. Protein cages are studied extensively for applications in nanotechnology, nanomedicine and materials science. Their inner and outer surfaces can be modified chemically or genetically, and the internal cavity can be used to template, store and/or arrange molecular cargos. Virus capsids and virus-like particles (VLP, noninfectious particles) provide versatile platforms for nanoscale bioengineering...
October 30, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29058656/bursal-transcriptome-profiling-of-different-inbred-chicken-lines-reveals-key-differentially-expressed-genes-at-3-days-post-infection-with-very-virulent-infectious-bursal-disease-virus
#11
Mohd Isa Farhanah, Abd Rahaman Yasmin, Nurulfiza Mat Isa, Mohd Hair-Bejo, Aini Ideris, Claire Powers, Omobolanle Oladapo, Venugopal Nair, Jia-Shiun Khoo, Ahmad-Kamal Ghazali, Wai-Yan Yee, Abdul Rahman Omar
Infectious bursal disease is a highly contagious disease in the poultry industry and causes immunosuppression in chickens. Genome-wide regulations of immune response genes of inbred chickens with different genetic backgrounds, following very virulent infectious bursal disease virus (vvIBDV) infection are poorly characterized. Therefore, this study aims to analyse the bursal tissue transcriptome of six inbred chicken lines 6, 7, 15, N, O and P following infection with vvIBDV strain UK661 using strand-specific next-generation sequencing, by highlighting important genes and pathways involved in the infected chicken during peak infection at 3 days post-infection...
October 23, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29053853/genome-sequence-analysis-of-a-distinctive-italian-infectious-bursal-disease-virus
#12
V Felice, G Franzo, E Catelli, A Di Francesco, M Bonci, M Cecchinato, G Mescolini, D Giovanardi, P Pesente, C Lupini
In a recent study, an emerging infectious bursal disease virus (IBDV) genotype (ITA) was detected in IBDV-live vaccinated broilers without clinical signs of infectious bursal disease (IBD). VP2 sequence analysis showed that strains of the ITA genotype clustered separately from vaccine strains and from other IBDV reference strains, either classic or very virulent. In order to obtain a more exhaustive molecular characterization of the IBDV ITA genotype and speculate on its origin, genome sequencing of the field isolate IBDV/Italy/1829/2011, previously assigned to the ITA genotype, was performed, and the sequences obtained were compared to the currently available corresponding sequences...
October 10, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053823/effect-of-maternally-derived-antibody-on-sequential-infection-with-highly-virulent-bursal-disease-virus-in-newly-hatched-broilers
#13
W J Jeon, H J Lee, I S Roh, Y S Yoon, J H Kim, K S Choi
Maternally derived antibodies (MDAs) are important for protection against very virulent infectious bursal disease virus (vvIBDV). In this study, 5-day-old commercial broilers with non-uniform MDA titers (with a coefficient of variation of 50%) were challenged with vvIBDV and given free contact with each other during a 2-week period. The chicks were assigned to four MDA-titer subgroups, GI-1 (very low MDA), GI-2 (low MDA), GI-3 (medium MDA), and GI-4 (high MDA). Transient symptoms of infection were observed in 35...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29046449/gga-mir-130b-suppresses-infectious-bursal-disease-virus-replication-via-targeting-the-viral-genome-and-cellular-socs5
#14
Mengjiao Fu, Bin Wang, Xiang Chen, Zhiyuan He, Yongqiang Wang, Xiaoqi Li, Hong Cao, Shijun J Zheng
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post transcriptionally through silencing or degrading its targets, playing important roles in immune response. However, the role of miRNAs in host response against infectious bursal disease virus (IBDV) infection is not clear. In this study, we show that the expression of a series of miRNAs was significantly altered in DF-1 cells after infectious bursal disease virus (IBDV) infection. We found that gga-miR-130b inhibited IBDV replication via targeting the specific sequence of IBDV segment A and enhanced the expression of IFN-β by targeting Suppressors of Cytokine Signaling 5(SOCS5) in host cells...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29039212/propagation-and-titration-of-infectious-bursal-disease-virus-including-non-cell-culture-adapted-strains-using-ex-vivo-stimulated-chicken-bursal-cells
#15
Sebastien Mathieu Soubies, Céline Courtillon, Mouna Abed, Michel Amelot, Alassane Keita, Andrew Broadbent, Sonja Härtle, Bernd Kaspers, Nicolas Eterradossi
Infectious bursal disease virus (IBDV) is a Birnaviridae family member of economic importance for poultry. This virus infects and destroys developing B lymphocytes in the cloacal bursa, resulting in a potentially fatal or immunosuppressive disease in chickens. Naturally-occurring viruses and many vaccine strains are not able to grow in in vitro systems without prior adaptation, which process often affects viral properties such as virulence. Primary bursal cells, which are the main target cells of lymphotropic IBDV in vivo, may represent an attractive system for the study of IBDV...
October 17, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28988058/the-down-regulation-of-casein-kinase-1-alpha-as-a-host-defense-response-against-infectious-bursal-disease-virus-infection
#16
Lizhou Zhang, Hui Li, Yuming Chen, Xiang Gao, Zhen Lu, Li Gao, Yongqiang Wang, Yulong Gao, Honglei Gao, Changjun Liu, Hongyu Cui, Yanping Zhang, Qing Pan, Xiaole Qi, Xiaomei Wang
Infectious bursal disease virus (IBDV) is an important immunosuppressive virus of chickens. Although the gene functions of IBDV have been well characterized, the host responses during IBDV infection remain much poor. In the present study, casein kinase 1 alpha (CK1α), a novel VP2-associated protein, was down-regulated during IBDV replication in DF1 cells. Further experiments showed that siRNA-mediated knockdown of CK1α inhibited IBDV replication, while overexpression of CK1α promoted IBDV growth. Finally, we revealed that the effects of CK1α expression level on IBDV replication were involved in the negative regulation of CK1α on type I interferon receptor (IFNAR1), because ubiquitination assay analyses demonstrated that CK1α could promote the ubiquitination of IFNAR1, thereby affecting the stability of this receptor...
December 2017: Virology
https://www.readbyqxmd.com/read/28972403/detection-of-different-genotypes-of-infectious-bronchitis-virus-and-of-infectious-bursal-disease-virus-in-european-broilers-during-an-epidemiological-study-in-2013-and-the-consequences-for-the-diagnostic-approach
#17
J J de Wit, C Cazaban, R Dijkman, G Ramon, Y Gardin
To gather recent data regarding the infectious bronchitis (IB) and infectious bursal disease (IBD) situation in Europe, a large-scale field epidemiological survey using diagnostic samples has been implemented in 2013 for about six months in several European countries: France, Germany, Greece, Italy, the Netherlands, Poland, Portugal, the Republic of Ireland, Spain and the United Kingdom. In 234 flocks that were sampled, strains from 10 different IBV genotypes were detected: the 793B genotype was detected most frequently, followed by QX, Massachusetts (Mass) and the Xindadi-like strains...
October 3, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28954433/newcastle-disease-virus-vectored-bivalent-vaccine-against-virulent-infectious-bursal-disease-and-newcastle-disease-of-chickens
#18
Sohini Dey, Madhan Mohan Chellappa, Dinesh C Pathak, Satish Gaikwad, Kalpana Yadav, Saravanan Ramakrishnan, Vikram N Vakharia
Newcastle disease virus (NDV) strain F is a lentogenic vaccine strain used for primary vaccination in day-old chickens against Newcastle disease (ND) in India and Southeast Asian countries. Recombinant NDV-F virus and another recombinant NDV harboring the major capsid protein VP2 gene of a very virulent infectious bursal disease virus (IBDV); namely rNDV-F and rNDV-F/VP2, respectively, were generated using the NDV F strain. The rNDV-F/VP2 virus was slightly attenuated, as compared to the rNDV-F virus, as evidenced from the mean death time and intracerebral pathogenicity index analysis...
September 26, 2017: Vaccines
https://www.readbyqxmd.com/read/28921008/molecular-characterization-of-very-virulent-infectious-bursal-disease-virus-strains-circulating-in-egypt-from-2003-to-2014
#19
Awad A Shehata, Hesham Sultan, Mohammed Y Halami, Shaimaa Talaat, Thomas W Vahlenkamp
In the present study, four very virulent infectious bursal disease virus (vvIBDV) isolates from flocks of chickens with vaccination failure in Egypt in 2003, 2007, 2010 and 2014 were characterized. The four viruses, designated USC2003, USC2007, USC2010 and USC2014, were detected by reverse transcription PCR, subjected to sequencing of both genomic segments (A and B) and compared with geographically and phylogenetically diverse IBDV strains. Phylogenetic analysis of segment A (complete) and B (partial) revealed a close relationship between Egyptian and vvIBDV reference strains of European and Asian origin...
December 2017: Archives of Virology
https://www.readbyqxmd.com/read/28839013/genome-sequence-of-a-novel-reassortant-and-very-virulent-strain-of-infectious-bursal-disease-virus
#20
Hyun-Jeong Lee, Il Jang, Sun-Hwa Shin, Hee-Soo Lee, Kang-Seuk Choi
Here, we report the complete coding genome sequence of a novel reassortant and very virulent infectious bursal disease virus (IBDV), designated JBN2011. Characterization of the JBN2011 genome suggests that it is a rare recombinant virus having a very virulent IBDV segment A and a Bursine-2-like attenuated IBDV segment B.
August 24, 2017: Genome Announcements
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