keyword
MENU ▼
Read by QxMD icon Read
search

7 keto

keyword
https://www.readbyqxmd.com/read/29460974/significant-alterations-of-6-keto-prostaglandin-f-1a-and-no-levels-in-spermatic-vein-plexus-patients-with-varicocele
#1
L Tian, H Han, H-E Lei, X-S Qian, S-J Feng, X-D Zhang
To investigate the level variation of correlative factors between the spermatic vein plexus and peripheral blood in patients with varicocele, a total of 22 patients diagnosed with varicocele were enrolled in the study. All patients were performed a testicular artery-sparing microsurgical varicocelectomy. During the operation, a blood sample from the left spermatic vein plexus and a peripheral blood sample were collected. A radioimmunoassay was used to determine the 6-keto prostaglandin F1a (6-keto-PGF1a ). A colorimetric method was performed to determine the NO...
February 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29438900/sperm-quality-biomarkers-complement-reproductive-and-endocrine-parameters-in-investigating-environmental-contaminants-in-common-carp-cyprinus-carpio-from-the-lake-mead-national-recreation-area
#2
Jill A Jenkins, Michael R Rosen, Rassa O Draugelis-Dale, Kathy R Echols, Leticia Torres, Carla M Wieser, Constance A Kersten, Steven L Goodbred
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp (Cyprinus carpio) gamete quality and endocrine- and reproductive parameters, data were collected among sub-basins over 7 years (1999-2006)...
February 10, 2018: Environmental Research
https://www.readbyqxmd.com/read/29435719/adaptation-of-the-secretome-of-echinostoma-caproni-may-contribute-to-parasite-survival-in-a-th1-milieu
#3
Alba Cortés, Carla Muñoz-Antolí, María Álvarez-Izquierdo, Javier Sotillo, J Guillermo Esteban, Rafael Toledo
Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, broadly employed to study the host-dependent mechanisms that govern the evolution of intestinal helminth infections. Resistance against E. caproni homologous secondary infections has been reported in mice and appears to be related to the generation of a local Th2 response, whereas Th1 responses promote the development of chronic primary infections. Herein, the ability of E. caproni to modulate its secretome according to the host environment is investigated...
February 12, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29435611/electrophilic-derivatives-of-omega-3-fatty-acids-counteract-lung-cancer-cell-growth
#4
Liboria Siena, Chiara Cipollina, Serena Di Vincenzo, Maria Ferraro, Andreina Bruno, Mark Gjomarkaj, Elisabetta Pace
PURPOSE: 17-oxo-DHA is an electrophilic keto-derivative of the omega-3 fatty acid docosahexaenoic acid (DHA) endogenously generated by cyclooxygenase-2 and a cellular dehydrogenase. 17-oxo-DHA displays anti-inflammatory and cytoprotective actions. DHA, alone or in combination with standard chemotherapy, displays antitumor activity. However, the effects of electrophilic keto-derivatives of DHA on cancer growth have never been evaluated. We investigated whether 17-oxo-DHA, alone or in combination with gemcitabine, displayed antitumor effects...
February 12, 2018: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29378089/impaired-sperm-function-in-infertile-men-relies-on-the-membrane-sterol-pattern
#5
A Garolla, I Šabović, S Tescari, L De Toni, M Menegazzo, I Cosci, V De Filippis, M Giarola, C Foresta
Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation. However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated. To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects...
January 29, 2018: Andrology
https://www.readbyqxmd.com/read/29361712/protective-mechanism-of-the-antioxidant-baicalein-toward-hydroxyl-radical-treated-bone-marrow-derived-mesenchymal-stem-cells
#6
Yage Tian, Xican Li, Hong Xie, Xiaozhen Wang, Yulu Xie, Chuanbing Chen, Dongfeng Chen
Our study explores the antioxidant and cytoprotective effects of baicalein and further discusses the possible mechanisms. A methyl thiazolyl tetrazolium (MTT) assay revealed that baicalein could considerably enhance the viability of hydroxyl radical-treated bone marrow-mesenchymal stem cells (bmMSCs) at 37-370 µM. The highest viability rate was 120.4%. In subsequent studies, baicalein was observed to effectively scavenge hydroxyl radical and PTIO• radicals, reducing Fe3+ and Cu2+ ions. In the Fe2+-chelating UV-vis spectra, mixing of baicalein with Fe2+ yielded two evident redshifts (275 → 279 nm and 324 → 352 nm) and a broad absorption peak (λmax ≈ 650 nm, ε = 1...
January 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29297772/assessment-of-drug-drug-interaction-potential-and-pbpk-modeling-of-cc-223-a-potent-inhibitor-of-the-mammalian-target-of-rapamycin-kinase
#7
Zeen Tong, Rangaraj Narayanan, Christian Atsriku, Jim Nissel, Yan Li, Hong Liu, Xiaomin Wang, Sekhar Surapaneni
1. CC-223 was studied in vitro for metabolism and drug-drug interactions (DDI), and in clinic for interaction with ketoconazole. 2. In vitro, human metabolites of CC-223 included O-desmethyl CC-223 (M1), keto (M2), N-oxide (M3), and imine (M13), with M1 being the most prominent metabolite. 3. CC-223 was metabolized by CYP2C9 and CYP3A, while metabolism of M1 was mediated by CYP2C8 and CYP3A. Ketoconazole increased CC-223 and M1 exposure by 60-70% in healthy volunteers. 4. CC-223 (IC50 ≥ 27 µM) and M1 (IC50 ≥ 46 µM) were inhibitors of CYP2C9 and CYP2C19 in human liver microsomes...
January 3, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29283703/growth-of-yersinia-pseudotuberculosis-strains-at-different-temperatures-ph-values-and-nacl-and-ethanol-concentrations
#8
Riikka Keto-Timonen, Anna Pöntinen, Mariella Aalto-Araneda, Hannu Korkeala
Maximum growth temperature and growth limits in Luria-Bertani broth at different pH values and NaCl and ethanol concentrations were determined for 49 Yersinia pseudotuberculosis strains representing serotypes O:1, O:2, O:3, O:4, and O:5. In addition, the ability of the strains to grow at 0°C and the growth parameters at 1°C were determined. The maximum growth temperatures measured by Gradiplate temperature incubator varied between 42.2 and 43.7°C. All strains were able to grow at 0°C in Luria-Bertani broth within 17 days of incubation...
January 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29282829/electrochemical-oxidation-of-phosphatidylethanolamines-studied-by-mass-spectrometry
#9
Simone Colombo, Giulia Coliva, Agnieszka Kraj, Jean-Pierre Chervet, Maria Fedorova, Pedro Domingues, M Rosário Domingues
Phosphatidylethanolamines (PEs) are widely present in cellular membranes and lipoproteins. Oxidation of PEs fatty acyl chains generates several oxidized products, exerting a vast number of biological functions, not totally unveiled yet. In vitro biomimetic models have been used to identify oxidized PEs and to develop analytical strategies for their targeted detection in vivo. Most of the models are based on oxidation by reactive oxygen species (ROS), but the oxidative metabolism of PE also relies on controlled reactions catalyzed by enzymes as lipoxygenase (LOX), which can be mimicked by electrochemical (EC) oxidation...
December 28, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29277660/properties-and-mechanism-of-d-glucosaminate-6-phosphate-ammonia-lyase-an-aminotransferase-family-enzyme-with-d-amino-acid-specificity
#10
Robert S Phillips, Samuel C Ting, Ange G Tetsadjio, Kaitlin L Anderson, Kyle M Friez, Katherine A Miller, Timothy R Hoover
Salmonella enterica serovar Typhimurium utilizes a wide range of growth substrates, some of which are relatively novel. One of these unusual substrates is d-glucosaminate, which is metabolized by the enzymes encoded in the dga operon. d-Glucosaminate is transported and converted to d-glucosaminate-6-phosphate (G6P) by a phosphotransferase system, composed of DgaABCD. The protein product of dgaE, d-glucosaminate-6-phosphate ammonia lyase (DGL), converts G6P to 2-keto-3-deoxygluconate-6-phosphate, which undergoes a retroaldol reaction catalyzed by the DgaF protein to give d-glyceraldehyde-3-phosphate and pyruvate...
December 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29248502/a-continuous-assay-for-l-talarate-galactarate-dehydratase-using-circular-dichroism
#11
Nicole M Easton, Sarah A E Aboushawareb, Stephen L Bearne
l-Talarate/galactarate dehydratase (TGD) is a member of the enolase superfamily of enzymes and catalyzes the dehydration of either meso-galactarate or l-talarate to form 5-keto-4-deoxy-d-glucarate (5-KDG). To facilitate study of this enzyme and other galactarate dehydratases, a continuous circular dichroism-based assay has been developed. Using recombinant enzyme from Salmonella typhimuium (StTGD), the rates of StTGD-catalyzed conversion of m-galactarate to 5-KDG were determined by following the change in ellipticity at 323 nm...
December 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29191105/curcumin-and-its-derivatives-inhibit-2-3-7-8-tetrachloro-dibenzo-p-dioxin-induced-expression-of-drug-metabolizing-enzymes-through-aryl-hydrocarbon-receptor-mediated-pathway
#12
Rika Nakai, Shun Fukuda, Masaya Kawase, Yoko Yamashita, Hitoshi Ashida
Certain dioxins, including 2,3,7,8,-tetrachloro-dibenzo-p-dioxin (TCDD), are exogenous ligands for an aryl hydrocarbon receptor (AhR) and induces various drug-metabolizing enzymes. In this study, we examined the effect of curcumin on expression of drug-metabolizing enzymes through the AhR and NF-E2 related factor 2 (Nrf2) pathways. Curcumin dose-dependently inhibited TCDD-induced expression of phase I enzyme cytochrome P450 1A1 (CYP1A1) and phase II enzymes NAD(P)H:quinone oxidoreductase-1 (NQO1) and heme oxygenase 1 (HO-1) but not tert-butyl hydroquinone-induced NQO1 and HO-1, suggesting that curcumin inhibited only AhR pathway, but not Nrf2 one directly...
December 1, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29183124/new-triterpenoid-saponins-from-green-vegetable-soya-beans-and-their-anti-inflammatory-activities
#13
Xiuhua Lan, Kejun Deng, Jianping Zhao, Yiyi Chen, Xuhui Xin, Yanli Liu, Ikhlas A Khan, Shilin Yang, Taoyun Wang, Qiong Ming Xu
Ten compounds were isolated and identified from green vegetable soya beans, of which five triterpenoid saponins (1-5) are new, and five compounds (6-10) are known. The chemical structures of five triterpenoid saponins (1-5) were elucidated to be 3β,24-dihydroxy-22β,30-epoxy-30-oxoolean-12-en 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl-(1→2)-β-D-glucuronopyranoside, 1, 3β,24-dihydroxy-22β,30-epoxy-30-oxoolean-12-en 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-(3''-O-formyl)-galactopyranosyl-(1→2)-β-D-glucuronopyranoside, 2, 22-keto-3β,24-dihydroxy oleanane-12-ene 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-(3''-O-formyl)-galactopyranosyl-(1→2)-β-D-glucuronopyranoside, 3, 3β,22β,24-trihydroxy oxyolean-18(19)-ene-29-acid 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranoside, 4, and punicanolic acid 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranoside, 5, in terms of spectroscopic data (IR, GTC/FID, HR-ESI-MS, 1D and 2D NMR)...
November 29, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29182331/polyoxygenated-steroids-from-the-sponge-clathria-gombawuiensis
#14
Jung-Kyun Woo, Thi Kim Quy Ha, Dong-Chan Oh, Won-Keun Oh, Ki-Bong Oh, Jongheon Shin
Six new polyoxygenated steroids (1-6) along with clathriol (7) were isolated from the Korean marine sponge Clathria gombawuiensis. Based upon the results of combined spectroscopic analyses, the structures of gombasterols A-F (1-6) were elucidated to be those of highly oxygenated steroids possessing a 3β,4α,6α,7β-tetrahydroxy or equivalent (7β-sodium O-sulfonato for 3) substitution pattern and a C-15 keto group as common structural motifs. The relative and absolute configurations of these steroids, including the rare 14β configuration of 1-4, were determined by a combination of NOESY, J-based analyses, the 2-methoxy-2-(trifluoromethyl)phenylacetic acid (MTPA) method, and X-ray crystallographic analysis...
November 28, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29175486/targeting-the-15-keto-pge2-ptgr2-axis-modulates-systemic-inflammation-and-survival-in-experimental-sepsis
#15
Ing-Jung Chen, Siow-Wey Hee, Chun-Hsing Liao, Shih-Yao Lin, Lynn Su, Chia-Tung Shun, Lee-Ming Chuang
Sepsis is a systemic inflammation accompanied by multi-organ dysfunction due to microbial infection. Prostaglandins and their metabolites have long been studied for their importance in regulating the innate immune response. 15-keto-PGE2 (15k-PGE2) is a prostaglandin E2 (PGE2) metabolite, whose further processing is catalyzed by prostaglandin reductase 2 (PTGR2). We showed disruption of the Ptgr2 gene in mice improves the survival rate under both LPS- and cecum ligation/puncture (CLP)-induced experimental sepsis...
November 22, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29149663/%C3%AE-keto-tetrahydrofuran-lignan-glucosides-from-the-bangladeshi-medicinal-plant-terminalia-citrina-inhibit-estradiol-e2-induced-proliferation-in-cancer-cells
#16
Md Abdul Muhit, Kaoru Umehara, Hiroshi Noguchi
EtOAc extract from the leaves of Terminalia citrina collected in Bangladesh were separated, and seven previously undescribed α-keto tetrahydrofuran lignan glucosides (terminalosides Q to W) were isolated and characterized. NOESY analysis of (1)H NMR spectra and ECD spectroscopic data analysis revealed the absolute stereochemistry of the tetrahydrofuran ring of the isolated constituents as being a (7S,8R,8'S)- configuration in terminalosides Q to U and a (7R,8R,8'S)- configuration in terminalosides V and W...
November 14, 2017: Phytochemistry
https://www.readbyqxmd.com/read/29132038/hemodynamics-of-the-corpus-luteum-in-mares-during-experimentally-impaired-luteogenesis-and-partial-luteolysis
#17
J C Ferreira, L F Novaes Filho, Y L Boakari, H S Canesin, D L Thompson, F S Lima, C Meira
The aim of the current project was to characterize the luteal vascularity and the plasma concentrations of progesterone (P4), prolactin (PRL) and 13,14-dihydro-15-keto-PGF2α (PGFM) in mares with luteal disturbances during early and mid-diestrus. In Experiment 1, twenty-one mares were treated with 2 mL of 0.9% NaCl, or 1 mg Dinoprost, or 10 mg Dinoprost on day two after ovulation (Control-D2, 1/10PGF-D2 and PGF-D2 groups, respectively; n = 7 mares/group). In Experiment 2, similar treatments were performed eight days post-ovulation using a different cohort of 21 mares (Control-D8, 1/10PGF-D8 and PGF-D8 groups, respectively; n = 7 mares/group)...
November 4, 2017: Theriogenology
https://www.readbyqxmd.com/read/29132037/flunixin-meglumine-improves-pregnancy-rate-in-embryo-recipient-beef-cows-with-an-excitable-temperament
#18
R K Kasimanickam, J B Hall, C T Estill, J P Kastelic, C Joseph, R L Abdel Aziz, D Nak
Objectives were to determine effects of: 1) handling temperament and administration of flunixin meglumine, an inhibitor of prostaglandin F2a (PGF2a) synthesis, given at the time of embryo transfer, on pregnancy rates in beef cattle embryo transfer recipients; 2) handling temperament and flunixin meglumine on peripheral concentrations of progesterone, cortisol, substance-P, prostaglandin F metabolites (PGFM, (13,14-dihydro-15-keto-PGF2a) and isoprostane 8-epi PGF2a; and 3) flunixin meglumine treatment on proportion of non-pregnant recipient cows returning to estrus within an expected interval...
November 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/29128619/preparation-of-porous-carbon-spheres-from-2-keto-l-gulonic-acid-mother-liquor-by-oxidation-and-activation-for-electric-double-layer-capacitor-application
#19
Zhi-Qiang Hao, Jing-Pei Cao, Xiao-Yan Zhao, Yan Wu, Jun-Sheng Zhu, Ya-Li Dang, Qi-Qi Zhuang, Xian-Yong Wei
A novel strategy is proposed for the increase of specific surface area (SSA) of porous carbon sphere (PCS) by oxidation and activation. 2-keto-l-gulonic acid mother liquor (GAML) as a high-pollution waste has a relatively high value of reutilization. For its high value-added utilization, GAML is used as the precursor for preparation of PCS as carbon-based electrode materials for electric double-layer capacitor. PCS is prepared by hydrothermal carbonization, carbonization and KOH activation, and Fe(NO3)3 9H2O is used as an oxidizing agent during carbonization...
November 3, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29111689/functional-characterization-of-a-condensation-domain-that-links-nonribosomal-peptide-and-pteridine-biosynthetic-machineries-in-photorhabdus-luminescens
#20
Corey E Perez, Hyun Bong Park, Jason M Crawford
Nonribosomal peptide synthetases (NRPSs) produce a wide variety of biologically important small molecules. NRPSs can interface with other enzymes to form hybrid biosynthetic systems that expand the structural and functional diversity of their products. The pepteridines are metabolites encoded by an unprecedented pteridine-NRPS-type hybrid biosynthetic gene cluster in Photorhabdus luminescens, but how the distinct enzymatic systems interface to produce these molecules has not been examined at the biochemical level...
November 7, 2017: Biochemistry
keyword
keyword
77076
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"