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transcription factor 7-like 2

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https://www.readbyqxmd.com/read/27919948/long-noncoding-rna-snar-regulates-proliferation-migration-and-invasion-of-triple-negative-breast-cancer-cells
#1
Jeeyeon Lee, Jin Hyang Jung, Yee Soo Chae, Ho Yong Park, Wan Wook Kim, Soo Jung Lee, Jae-Hwan Jeong, Seung Hee Kang
AIM: We evaluated the role of long noncoding ribonucleic acid (lncRNA) in breast cancer cell lines by quantitative reverse transcription-polymerase change reaction. MATERIALS AND METHODS: The effects of small NF90-associated RNA (snaR) with RNA interference on proliferation, migration and invasion of MDA-MB-231 cells were observed by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide, wound healing and transwell assay. RESULTS: Among 90 lncRNAs, E2F transcription factor 4, p107/p130-binding (E2F4) antisense, insulin-like growth factor 2 antisense (IGF2AS), snaR, and small nucleolar RNA host gene 5 (SNHG5) were up-regulated in MDA-MB-231 and 7SK, antisense noncoding RNA in the INK4 locus (ANRIL), IGF2AS, Nespas, p53 mRNA, and snaR were up-regulated in MCF-7 cells...
December 2016: Anticancer Research
https://www.readbyqxmd.com/read/27916785/the-differentially-dna-methylated-region-responsible-for-expression-of-runt-related-transcription-factor-2
#2
Shoichi Wakitani, Daigo Yokoi, Yuichi Hidaka, Koichiro Nishino
Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study is aimed at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2...
December 4, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27906110/failed-upregulation-of-tfam-protein-and-mitochondrial-dna-in-oxidatively-deficient-fibers-of-chronic-obstructive-pulmonary-disease-locomotor-muscle
#3
Yana Konokhova, Sally Spendiff, R Thomas Jagoe, Sudhakar Aare, Sophia Kapchinsky, Norah J MacMillan, Paul Rozakis, Martin Picard, Mylène Aubertin-Leheudre, Charlotte H Pion, Jean Bourbeau, Russell T Hepple, Tanja Taivassalo
BACKGROUND: Low mitochondrial content and oxidative capacity are well-established features of locomotor muscle dysfunction, a prevalent and debilitating systemic occurrence in patients with chronic obstructive pulmonary disease (COPD). Although the exact cause is not firmly established, physical inactivity and oxidative stress are among the proposed underlying mechanisms. Here, we assess the impact of COPD pathophysiology on mitochondrial DNA (mtDNA) integrity, biogenesis, and cellular oxidative capacity in locomotor muscle of COPD patients and healthy controls...
February 18, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27902922/the-extent-to-which-immunity-apoptosis-and-detoxification-gene-expression-interact-with-17-alpha-methyltestosterone
#4
Haitham G Abo-Al-Ela, Abeer F El-Nahas, Shawky Mahmoud, Essam M Ibrahim
Innate immunity is the first line of defence against invasion by foreign pathogens. One widely used synthetic androgen for the production of all-male fish, particularly commercially valuable Nile tilapia, Oreochromis niloticus, is 17 alpha-methyltestosterone (MT). The present study investigates the effect of MT on innate immunity, cellular apoptosis and detoxification and the mortality rate, during and after the feeding of fry with 0-, 40-and 60-mg MT/kg. Expression analysis was completed on interleukin 1 beta (il1β), interleukin 8 (il8), tumour necrosis factor alpha (tnfα), CXC2- and CC-chemokines, interferon (ifn), myxovirus resistance (mx), toll-like receptor 7 (tlr7), immunoglobulin M heavy chain (IgM heavy chain), vitellogenin (vtg), cellular apoptosis susceptibility (cas) and glutathione S-transferase α1 (gstα1)...
November 27, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27899394/stress-activated-kinase-mkk7-governs-epigenetics-of-cardiac-repolarization-for-arrhythmia-prevention
#5
Sanjoy K Chowdhury, Wei Liu, Min Zi, Yatong Li, Shunyao Wang, Hoyee Tsui, Sukhpal Prehar, Simon J Castro, Henggui Zhang, Yong Ji, Xiuqin Zhang, Rui-Ping Xiao, Rongli Zhang, Ming Lei, Lukas Cyganek, Kaomei Guan, Catherine B Millar, Xudong Liao, Mukesh K Jain, Mark R Boyett, Elizabeth J Cartwright, Holly A Shiels, Xin Wang
BACKGROUND: -Ventricular arrhythmia is a leading cause of cardiac mortality. Most antiarrhythmics present paradoxical pro-arrhythmic side effects, culminating in a greater risk of sudden death. METHODS: -We describe a new regulatory mechanism linking mitogen-activated kinase kinase-7 (MKK7) deficiency with increased arrhythmia vulnerability in hypertrophied and failing hearts using mouse models harbouring MKK7 knockout or overexpression. The human relevance of this arrhythmogenic mechanism is evaluated in human induced pluripotent stem cells-derived cardiomyocytes (iPSC-CMs)...
November 29, 2016: Circulation
https://www.readbyqxmd.com/read/27889265/integration-of-phytochrome-and-cryptochrome-signals-determines-plant-growth-during-competition-for-light
#6
Mieke de Wit, Diederik H Keuskamp, Franca J Bongers, Patricia Hornitschek, Charlotte M M Gommers, Emilie Reinen, Carmen Martínez-Cerón, Christian Fankhauser, Ronald Pierik
Plants in dense vegetation perceive their neighbors primarily through changes in light quality. Initially, the ratio between red (R) and far-red (FR) light decreases due to reflection of FR by plant tissue well before shading occurs. Perception of low R:FR by the phytochrome photoreceptors induces the shade avoidance response [1], of which accelerated elongation growth of leaf-bearing organs is an important feature. Low R:FR-induced phytochrome inactivation leads to the accumulation and activation of the transcription factors PHYTOCHROME-INTERACTING FACTORs (PIFs) 4, 5, and 7 and subsequent expression of their growth-mediating targets [2, 3]...
November 17, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27876620/the-gabab-positive-allosteric-modulators-cgp7930-and-gs39783-stimulate-erk1-2-signalling-in-cells-lacking-functional-gabab-receptors
#7
Maria C Olianas, Simona Dedoni, Pierluigi Onali
The present study shows that the GABAB positive allosteric modulators (PAMs) CGP7930 and GS39783 stimulate extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) signalling in cells that do not express functional GABAB receptors. In human SH-SY5Y neuroblastoma cells, CGP7930 and GS39783 induced a time- and concentration-dependent increase in ERK1/2 phosphorylation with potencies similar to those displayed as GABAB PAMs. Conversely, γ-aminobutyric acid and the GABAB receptor agonists (-)baclofen and SKF97541 were completely inactive...
November 19, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27869499/capsaicinoids-improve-egg-production-by-regulating-ovary-nuclear-transcription-factors-against-heat-stress-in-quail
#8
N Sahin, C Orhan, M Tuzcu, V Juturu, K Sahin
1. To examine the molecular mechanism of capsaicinoid supplementation from Capsicum extract, laying Japanese quail (n=180, 5 weeks old) were reared either at 22(o)C for 24 h/d (thermoneutral, TN) or 34(o)C for 8 h/d (heat stress, HS) and fed on one of three diets containing 0, 25 or 50 mg of capsaicinoids per kg for 12 weeks (2x3 factorial arrangement). 2. The results revealed that exposure to HS decreased feed consumption by 10.7% and egg production by 13.6%, increased serum and ovary malondialdehyde (MDA) levels by 66...
November 21, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27844183/evaluation-and-treatment-of-endoplasmic-reticulum-er-stress-in-right-ventricular-dysfunction-during-monocrotaline-induced-rat-pulmonary-arterial-hypertension
#9
Jing-Jing Wang, Xiang-Rong Zuo, Jian Xu, Jin-Yong Zhou, Hui Kong, Xiao-Ning Zeng, Wei-Ping Xie, Quan Cao
PURPOSE: Endoplasmic reticulum (ER) stress contributes to pulmonary artery hypertension (PAH). However, the exact roles of ER stress in right ventricular (RV) dysfunction, which is strongly associated with PAH, are largely unknown. Here, we aimed to explore how ER stress affects RV function in a rat PAH model and evaluated the effects of an ER stress inhibitor on RV dysfunction. METHODS: We examined expression changes of an ER marker: chaperone glucose-regulated protein 78 (GRP78), three ER stress sensor proteins: activating transcription factor 6 (ATF6), inositol-requiring enzyme 1 (IRE1), and protein kinase RNA-like endoplasmic reticulum kinase (PERK), and a key ER stress-induced apoptosis indicator: CCAAT/enhancer-binding protein homologous protein (CHOP), with inflammation indicators: interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), and matrix metalloproteinases (MMPs) in RV at 3, 7, 14 and 28 days following a single dose of monocrotaline (MCT) injection, with or without a preventive treatment [4-phenylbutyric acid (PBA)]...
November 14, 2016: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/27832158/arnica-montana-stimulates-extracellular-matrix-gene-expression-in-a-macrophage-cell-line-differentiated-to-wound-healing-phenotype
#10
Marta Marzotto, Clara Bonafini, Debora Olioso, Anna Baruzzi, Laura Bettinetti, Francesca Di Leva, Elisabetta Galbiati, Paolo Bellavite
Arnica montana (Arnica m.) is used for its purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. This work tested Arnica m. effects on gene expression using an in vitro model of macrophages polarized towards a "wound-healing" phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c, 9c, 15c or Control...
2016: PloS One
https://www.readbyqxmd.com/read/27814396/chronic-activation-of-hepatic-nrf2-has-no-major-effect-on-fatty-acid-and-glucose-metabolism-in-adult-mice
#11
Sebastian Brachs, Angelika F Winkel, James Polack, Hui Tang, Maria Brachs, Daniel Margerie, Bodo Brunner, Kerstin Jahn-Hofmann, Hartmut Ruetten, Joachim Spranger, Dieter Schmoll
The transcription factor NF-E2-related factor 2 (Nrf2) induces cytoprotective genes, but has also been linked to the regulation of hepatic energy metabolism. In order to assess the pharmacological potential of hepatic Nrf2 activation in metabolic disease, Nrf2 was activated over 7 weeks in mice on Western diet using two different siRNAs against kelch-like ECH-associated protein 1 (Keap1), the inhibitory protein of Nrf2. Whole genome expression analysis followed by pathway analysis demonstrated successful knock-down of Keap1 expression and induction of Nrf2-dependent genes involved in anti-oxidative stress defense and biotransformation, proving the activation of Nrf2 by the siRNAs against Keap1...
2016: PloS One
https://www.readbyqxmd.com/read/27806760/-the-crosstalk-between-canonical-and-noncanonical-wnt-signaling-pathway-in-osteoblast-differentiation-of-periodontal-ligament-stem-cells-in-inflammatory-microenvironments
#12
N Liu, H G Shi, W Zhang, B Gu
Objective: To investigate the crosstalk between canonical Wnt/β-catenin and noncanonical Wnt/Ca(2+) pathway in osteoblast differentiation process of periodontal ligament stem cell (PDLSC) in inflammatory microenvironments. Methods: PDLSCs were obtained from human healthy individuals(H-PDLSC) and patients with periodontitis(P-PDLSC). The H/P-PDLSCs were transfected with β-catenin siRNA. Cell morphology was observed under fluorescent microscope and transfection efficiency was easured by Western blotting after transfection of PDLSC...
November 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27803314/promotion-of-adipogenesis-by-neuropeptide-y-during-the-later-stages-of-chicken-preadipocyte-differentiation
#13
Steven L Shipp, Mark A Cline, Elizabeth R Gilbert
Neuropeptide Y (NPY) promotes adipogenesis in both birds and mammals, although mechanisms in avians remain unclear. The objective of this study was thus to evaluate effects of NPY on chick preadipocyte proliferation and differentiation. Preadipocytes were treated with 0, 1, 10, or 100 nmol/L NPY and gene expression and cellular proliferation were evaluated at 12, 24, and 48 h. At 12 h posttreatment, mRNA abundance of topoisomerase II alpha (TOP2A), and thioredoxin-dependent peroxidase 2 was upregulated and NPY was downregulated in response to NPY (0 vs...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27798098/impaired-protein-stability-and-nuclear-localization-of-nobox-variants-associated-with-premature-ovarian-insufficiency
#14
Ilaria Ferrari, Justine Bouilly, Isabelle Beau, Fabiana Guizzardi, Alberto Ferlin, Marzia Pollazzon, Mariacarolina Salerno, Nadine Binart, Luca Persani, Raffaella Rossetti
Premature Ovarian Insufficiency (POI) is a clinical syndrome defined by a loss of ovarian activity before the age of 40. Its pathogenesis is still largely unknown, but increasing evidences support a genetic basis in most cases. Among these, heterozygous mutations in NOBOX, a homeobox gene encoding a transcription factor expressed specifically by oocyte and granulosa cells within the ovary, have been reported in ~ 6 % of women with sporadic POI. The pivotal role of NOBOX in early folliculogenesis is supported by findings in knock-out mice...
October 23, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27792933/association-between-the-tcf7l2-polymorphism-and-colorectal-cancer-does-not-differ-by-diabetes-and-obesity-statuses
#15
Hyun-Jeong Shim, Ran Lee, Min-Ho Shin, Hee-Nam Kim, Sun-Seog Kweon
This study evaluated the association between polymorphism in a newly identified locus, rs11196172, located in transcription factors 7-like 2 (TCF7L2) and colorectal cancer (CRC) risk according to diabetes and obesity statuses. A study enrolled 6138 CRC patients and 4367 community controls. The adjusted odds ratios (aORs) with age, sex, smoking, and body mass index of the A allele, compared with the G allele, was 1.08 (95% CI 1.01-1.16). The significantly higher risk of CRC with the A allele remained after adjusting for diabetic status (aOR 1...
December 2016: Cancer Epidemiology
https://www.readbyqxmd.com/read/27784498/-hypoxia-induced-the-remodeling-of-pulmonary-arterial-smooth-muscles-and-increased-the-pulmonary-artery-smooth-muscle-kr%C3%A3-ppel-like-zinc-finger-transcription-factor-5-expression
#16
M N Cao, Y Q Chen, Q Jiang, M C Li, W J Lu, J Wang
Objective: To study the effect of chronic hypoxia on pulmonary arterial remodeling and Krüppel-like zinc-finger transcription factor 5 (KLF5) protein expression in pulmonary artery smooth muscles in a hypoxia-induced pulmonary hypertension model. Methods: Totally 20 adult SD rats (200-250 g) were divided into a normoxia group and a chronic hypoxia group by the random number table. Rats in the chronic hypoxia group were put in an automatic hypoxia box for 21 days. After that, right ventricular systolic pressure (RVSP), mean right ventricular pressure (mRVP) and RV/(LV+ S) were measured...
October 12, 2016: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/27783115/aryl-hydrocarbon-receptor-signaling-modifies-toll-like-receptor-regulated-responses-in-human-dendritic-cells
#17
Sarah Kado, W L William Chang, Aimy Nguyen Chi, Monika Wolny, David M Shepherd, Christoph F A Vogel
Currently, it is not well understood how ligands of the aryl hydrocarbon receptor (AhR) modify inflammatory responses triggered by Toll-like receptor (TLR) agonists in human dendritic cells (DCs). Here, we show that AhR ligands 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the tryptophan derivatives 6-formylindolo[3,2-b] carbazole (FICZ), kynurenine (kyn), and the natural dietary compound indole-3-carbinol (I3C) differentially modify cytokine expression in human monocyte-derived DCs (MoDCs). The results show that TLR-activated MoDCs express higher levels of AhR and are more sensitive toward the effects of AhR ligands...
October 25, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27777306/a-critical-role-for-%C3%AE-catenin-in-modulating-levels-of-insulin-secretion-from-%C3%AE-cells-by-regulating-actin-cytoskeleton-and-insulin-vesicle-localization
#18
Brie Sorrenson, Emmanuelle Cognard, Kathryn L Lee, Waruni C Dissanayake, Yanyun Fu, Weiping Han, William E Hughes, Peter R Shepherd
The processes regulating glucose-stimulated insulin secretion (GSIS) and its modulation by incretins in pancreatic β-cells are only partly understood. Here we investigate the involvement of β-catenin in these processes. Reducing β-catenin levels using siRNA knockdown attenuated GSIS in a range of β-cell models and blocked the ability of GLP-1 agonists and the depolarizing agent KCl to potentiate this. This could be mimicked in both β-cell models and isolated islets by short-term exposure to the β-catenin inhibitory drug pyrvinium...
October 24, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27765088/-effects-of-local-transplantation-of-autologous-adipose-derived-mesenchymal-stem-cells-on-the-formation-of-hyperplastic-scar-on-rabbit-ears
#19
L Chen, D L Wang, Z R Wei, B Wang, J P Qi, G F Sun
Objective: To investigate the effects of local transplantation of autologous adipose-derived mesenchymal stem cells (ADSCs) on the formation of hyperplastic scar on rabbit ears. Methods: ADSCs were isolated from inguinal fat of six New Zealand rabbits and then sub-cultured. ADSCs of the third passage of each rabbit were used in the following experiments. Six full-thickness skin defect wounds with diameter of 6 mm on the ventral surface of every rabbit ear were made. Wound healing and local-tissue proliferation were observed, and complete epithelization time of wounds and formation time of hyperplastic scar were recorded...
October 20, 2016: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/27761137/selection-of-reliable-reference-genes-for-gene-expression-analysis-under-abiotic-stresses-in-the-desert-biomass-willow-salix-psammophila
#20
Jianbo Li, Huixia Jia, Xiaojiao Han, Jin Zhang, Pei Sun, Mengzhu Lu, Jianjun Hu
Salix psammophila is a desert shrub willow that has extraordinary adaptation to abiotic stresses and plays an important role in maintaining local ecosystems. Moreover, S. psammophila is regarded as a promising biomass feedstock because of its high biomass yields and short rotation coppice cycle. However, few suitable reference genes (RGs) for quantitative real-time polymerase chain reaction (qRT-PCR) constrain the study on normalization of gene expression in S. psammophila until now. Here, we investigated the expression stabilities of 14 candidate RGs across tissue types and under four abiotic stress treatments, including heat, cold, salt, and drought treatments...
2016: Frontiers in Plant Science
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