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Infectious bronchitis virus

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https://www.readbyqxmd.com/read/28646651/use-of-sequence-independent-single-primer-amplification-sispa-for-rapid-detection-identification-and-characterization-of-avian-rna-viruses
#1
Klaudia Chrzastek, Dong-Hun Lee, Diane Smith, Poonam Sharma, David L Suarez, Mary Pantin-Jackwood, Darrell R Kapczynski
Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination with MiSeq platform was applied to target negative- and positive-sense single-stranded RNA viral sequences. This method allowed successful sequence assembly of full or near full length avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) viral genome...
June 21, 2017: Virology
https://www.readbyqxmd.com/read/28633386/oral-administration-of-tea-saponins-to-relive-oxidative-stress-and-immune-suppression-in-chickens
#2
X Chi, S Bi, W Xu, Y Zhang, S Liang, S Hu
The present study was designed to evaluate the effects of tea saponins on oxidative stress induced by cyclophosphamide in chickens. One hundred twenty chickens were randomly divided into 5 groups. Groups 3 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress and immunosuppression. After that, groups 2 and 4 were orally administered tea saponins in drinking water for 7 d. Then, groups 1 to 4 were immunized with a live, bivalent vaccine of Newcastle disease virus and infectious bronchitis virus...
June 15, 2017: Poultry Science
https://www.readbyqxmd.com/read/28619140/decreased-egg-production-in-laying-hens-associated-with-infection-with-genotype-3-avian-hepatitis-e-virus-strain-from-china
#3
Qin Zhao, Baoyuan Liu, Yani Sun, Taofeng Du, Yiyang Chen, Xinjie Wang, Huixia Li, Yuchen Nan, Gaiping Zhang, En-Min Zhou
To determine the relationship between decreased egg production and avian HEV infection, thirty healthy 23-week-old Hy-Line Variety Brown layer hens were randomly divided into 3 groups with 10 hens per group. Next, a genotype 3 avian HEV strain from China was used to inoculate laying hens via oronasal or intravenous routes using a 50% chicken infectious dose of 500. All hens were necropsied at 14 weeks postinoculation (wpi). Fecal virus shedding, viremia, seroconversion, serum alanine aminotransferase (ALT) increases and liver lesions showed that after intravenous (i...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619139/analysis-of-antigenicity-and-pathogenicity-reveals-major-differences-among-qx-like-infectious-bronchitis-viruses-and-other-serotypes
#4
Shihong Yan, Xiaoyu Liu, Jing Zhao, Gang Xu, Ye Zhao, Guozhong Zhang
Avian coronavirus infectious bronchitis virus (IBV) causes considerable damage to the poultry industry worldwide and the proportion of QX-like genotype isolates have increased over time. Here, to better understand the antigenicity and pathogenicity of this genotype, we conducted sequence analyses, cross neutralization tests, and also examined the pathogenicity of two strains, SD and SZ. Sequence analyses revealed that SD and SZ isolates belong to the QX-like IBV genotype and share high homology in their full-length genomes...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619134/genetic-antigenic-and-pathogenic-characteristics-of-avian-infectious-bronchitis-viruses-genotypically-related-to-793-b-in-china
#5
Zongxi Han, Wenjun Zhao, Yuqiu Chen, Qianqian Xu, Junfeng Sun, Tingting Zhang, Yan Zhao, Shuling Liang, Mengying Gao, Qiuling Wang, Xiangang Kong, Shengwang Liu
In this study, 20 infectious bronchitis virus (IBV) strains, which were genotypically related to 793/B, as assessed by an S1 gene comparison and a complete genomic sequence analysis, were isolated and identified from 2009 to 2014 in China. Phylogenetic analysis, network tree, similarity plot analysis, Recombination Detection Program 4(RDP4) and sequence comparison revealed that 12 of the 20 isolates were likely the reisolated vaccine virus. One isolate, ck/CH/LSD/110857, was shown to have originated from recombination events between H120- and 4/91-like vaccine strains that did not result in changes of antigenicity and pathogenicity...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619132/co-infection-of-h9n2-subtype-avian-influenza-virus-and-infectious-bronchitis-virus-decreases-sp-a-expression-level-in-chickens
#6
Qi Huang, Kai Wang, Ling Pan, Kezong Qi, Hongmei Liu, Hongjun Chen
Chicken surfactant protein A (cSP-A) is a collectin believed to play an important role in antiviral immunity. However, cSP-A expression in the respiratory tract of chickens after viral co-infection remains unclear. The aim of this study was the detection and characterization of cSP-A in co-infected chickens. For this purpose, four-week-old specific pathogen-free (SPF) chickens were divided into five groups and inoculated intranasally with H9N2 subtype avian influenza virus (AIV), infectious bronchitis virus (IBV), or Newcastle disease virus (NDV)...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28606144/a-reverse-genetics-system-for-avian-coronavirus-infectious-bronchitis-virus-based-on-targeted-rna-recombination
#7
Steven J van Beurden, Alinda J Berends, Annika Krämer-Kühl, Dieuwertje Spekreijse, Gilles Chénard, Hans-Christian Philipp, Egbert Mundt, Peter J M Rottier, M Hélène Verheije
BACKGROUND: Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide. Major advances in the study of the molecular biology of IBV have resulted from the development of reverse genetics systems for the highly attenuated, cell culture-adapted, IBV strain Beaudette. However, most IBV strains, amongst them virulent field isolates, can only be propagated in embryonated chicken eggs, and not in continuous cell lines...
June 12, 2017: Virology Journal
https://www.readbyqxmd.com/read/28603519/antigen-sparing-and-enhanced-efficacy-of-multivalent-vaccines-adjuvanted-with-immunopotentiators-in-chickens
#8
Peipei Wu, Jihu Lu, Lei Feng, Hongzhuan Wu, Xuehua Zhang, Mei Mei, Jibo Hou, Xiufan Liu, Yinghua Tang
We previously described that immunopotentiators, CVCVA5, increased the efficacy of H5 and H9 subtype avian influenza vaccines in chickens, ducks, and geese. In this study, we further investigated the effects of the CVCVA5 for improving the efficacy of other univalent or multivalent inactivated vaccines. The immune response administrated with half-dose of monovalent vaccine plus CVCVA5 were higher than those of one dose of monovalent vaccine without immunopotentiators as measured by levels of antibodies from serum, tears and bronchoalveolar lavage fluids, and cytokines of IFNγ and IL-4 from serum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28523921/combination-of-h120-and-1-96-avian-infectious-bronchitis-virus-vaccine-strains-protect-chickens-against-challenge-with-is-1494-06-variant-2-like-infectious-bronchitis-virus
#9
M Habibi, V Karimi, A G Langeroudi, S A Ghafouri, M Hashemzadeh, R K Farahani, H Maghsoudloo, H Abdollahi, P Seifouri
Avian infectious bronchitis (IB) is a worldwide chicken disease, caused by avian infectious bronchitis virus (IBV) which infects all commercial poultry lines. The present study was done to evaluate protection caused by two different serotype vaccines (Massachusetts and 793/B) in order to evaluate protection against challenge with IS/1494/06-like virus (variant 2-like virus), which is prevalent in the Middle East. SPF chickens were divided into four groups (n = 20). First and second group as negative control group and non-vaccinated-challenged group received no vaccine...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28508215/reverse-genetics-system-for-the-avian-coronavirus-infectious-bronchitis-virus
#10
Erica Bickerton, Sarah M Keep, Paul Britton
We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. Here, we describe the use of transient dominant selection as a method for introducing modifications into the IBV cDNA that has been successfully used for the substitution of specific nucleotides, deletion of genomic regions, and exchange of complete genes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28495648/complete-genome-sequences-of-two-avian-infectious-bronchitis-viruses-isolated-in-egypt-evidence-for-genetic-drift-and-genetic-recombination-in-the-circulating-viruses
#11
Hassanein H Abozeid, Anandan Paldurai, Sunil K Khattar, Manal A Afifi, Magdy F El-Kady, Ayman H El-Deeb, Siba K Samal
Avian infectious bronchitis virus (IBV) is highly prevalent in chicken populations and is responsible for severe economic losses to poultry industry worldwide. In this study, we report the complete genome sequences of two IBV field strains, CU/1/2014 and CU/4/2014, isolated from vaccinated chickens in Egypt in 2014. The genome lengths of the strains CU/1/2014 and CU/4/2014 were 27,615 and 27,637 nucleotides, respectively. Both strains have a common genome organization in the order of 5'-UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-UTR-poly(A) tail-3'...
May 8, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28493279/specific-detection-of-gii-1-lineage-of-infectious-bronchitis-virus
#12
Katarzyna Domanska-Blicharz, Anna Lisowska, Anna Pikuła, Joanna Sajewicz-Krukowska
Infectious bronchitis virus (IBV) is a worldwide prevalent RNA virus that cause highly contagious and economically devastating disease in chicken. The virus exists in many different genetic forms which made the disease control very difficult. The present study describes the development and validation of TaqMan-probe-based real-time reverse transcription-polymerase chain reaction (real time RT-PCR) targeting the S1 coding region of S gene characteristic for the GII-1 lineage (formerly the D1466-like variant) of IBV...
May 10, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28473895/pathogenicity-study-of-iranian-genotype-of-avian-infectious-bronchitis-virus-ir-1
#13
Hamideh Najafi, Arash Ghalyanchi Langeroudi, Masoud Hashemzadeh, Vahid Karimi, Omid Madadgar, Reza Khaltabadi Farahani, Seyed Ali Ghafouri, Hossein Maghsoudloo, Parvaneh Seifouri, Ali Madhi
Avian infectious bronchitis (IB) is a major cause of economic losses in poultry industry. The IB virus primarily affects respiratory tract, but various strains differ in their tropism for other target organs such as kidney and alimentary tract. The objective of this study was to estimate the pathogenicity of Iranian IBV variant (IR-1), which is limited exclusively to Iran. Specific pathogen free chicks were inoculated intranasally. Sera, fecal swabs and different tissue samples were collected on different days post infection (DPI)...
2017: Veterinary Research Forum
https://www.readbyqxmd.com/read/28472110/different-evolutionary-trajectories-of-vaccine-controlled-and-non-controlled-avian-infectious-bronchitis-viruses-in-commercial-poultry
#14
Mark W Jackwood, Dong-Hun Lee
To determine the genetic and epidemiological relationship of infectious bronchitis virus (IBV) isolates from commercial poultry to attenuated live IBV vaccines we conducted a phylogenetic network analysis on the full-length S1 sequence for Arkansas (Ark), Massachusetts (Mass) and Delmarva/1639 (DMV/1639) type viruses isolated in 2015 from clinical cases by 3 different diagnostic laboratories. Phylogenetic network analysis of Ark isolates showed two predominant groups linked by 2 mutations, consistent with subpopulations found in commercial vaccines for this IBV type...
2017: PloS One
https://www.readbyqxmd.com/read/28447340/marginal-deficiencies-of-dietary-arginine-and-methionine-could-suppress-growth-performance-and-immunological-responses-in-broiler-chickens
#15
R Jahanian, M Khalifeh-Gholi
The present study was conducted to investigate the effects of different levels of dietary arginine (Arg) and methionine (Met) on performance, immune responses, and carcass characteristics of broiler chickens. A total of 540 day-old Ross 308 broiler chicks were randomly assigned into the nine experimental diets, consisting five replicates of 12 birds each. Dietary treatments included three different levels (90%, 100%, and 110% of National Research Council [NRC] specifications) of either dietary Arg or Met, which were fed to the birds according to a 3 × 3 factorial arrangement of treatments during a 42 days feeding trial...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28437156/development-of-a-taqman-based-real-time-pcr-assay-for-rapid-and-specific-detection-of-fowl-aviadenovirus-serotype-4
#16
Jianchang Wang, Jinfeng Wang, Ping Chen, Libing Liu, Wanzhe Yuan
Twelve serotypes of fowl aviadenovirus, namely, FAdV-(1-8a and 8b-11), have been identified, among which FAdV-4 is the aetiologic agent of hepatitis hydropericardium syndrome (HHS) in chickens. Outbreaks of HHS have been documented in many countries, causing significant economic losses. Real-time PCR methods described so far in the literature cross-detect different serotypes of FAdVs. In this study, we aimed to develop a TaqMan-based real-time PCR assay for the specific detection of FAdV-4. A pair of primers targeting the hexon gene and a TaqMan probe were designed...
June 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28419351/the-effect-of-diatomaceous-earth-in-live-attenuated-infectious-bronchitis-vaccine-immune-responses-and-protection-against-challenge
#17
Ali Nazmi, Rüdiger Hauck, Lynette B Corbeil, Rodrigo A Gallardo
Live virus vaccines are commonly used in poultry production, particularly in broilers. Massive application and generation of a protective local mucosal and humoral immunity with no adverse effects is the main goal for this strategy. Live virus vaccines can be improved by adding adjuvants to boost mucosal innate and adaptive responses. In a previous study we showed that diatomaceous earth (DE) can be used as adjuvant in inactivated vaccines. The aim of this study was to test DE as adjuvant in an Ark-DPI live infectious bronchitis virus (IBV) vaccine after ocular or spray application...
April 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28390158/lithium-chloride-inhibits-early-stages-of-foot-and-mouth-disease-virus-fmdv-replication-in-vitro
#18
Fu-Rong Zhao, Yin-Li Xie, Ze-Zhong Liu, Jun-Jun Shao, Shi-Fang Li, Yong-Guang Zhang, Hui-Yun Chang
Foot-and-mouth disease virus (FMDV) causes an economically important and highly contagious disease of cloven-hoofed animals such as cattle, swine, and sheep. FMD vaccine is the traditional way to protect against the disease, which can greatly reduce its occurrence. However, the use of FMD vaccines to protect early infection is limited. Therefore, the alternative strategy of applying antiviral agents is required to control the spread of FMDV in outbreak situations. As previously reported, LiCl has obviously inhibition effects on a variety of viruses such as transmissible gastroenteritis virus (TGEV), infectious bronchitis coronavirus (IBV), and pseudorabies herpesvirus and EV-A71 virus...
April 8, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28388925/a-robust-and-cost-effective-approach-to-sequence-and-analyze-complete-genomes-of-small-rna-viruses
#19
Kiril M Dimitrov, Poonam Sharma, Jeremy D Volkening, Iryna V Goraichuk, Abdul Wajid, Shafqat Fatima Rehmani, Asma Basharat, Ismaila Shittu, Tony M Joannis, Patti J Miller, Claudio L Afonso
BACKGROUND: Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsuspected variants and co-infecting agents. However, NGS is not widely used for small RNA viruses because of incorrectly perceived cost estimates and inefficient utilization of freely available bioinformatics tools...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28381285/new-oligonucleotide-microarray-for-rapid-diagnosis-of-avian-viral-diseases
#20
Kulyaisan T Sultankulova, Nurlan S Kozhabergenov, Vitaliy M Strochkov, Yerbol D Burashev, Kamshat A Shorayeva, Olga V Chervyakova, Nurkuisa M Rametov, Nurlan T Sandybayev, Abylay R Sansyzbay, Mukhit B Orynbayev
BACKGROUND: We developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV), and infectious bursal disease virus (IBDV) in single- and mixed-virus infections. The objective of the study was to develop an oligonucleotide microarray for rapid diagnosis of avian diseases that would be used in the course of mass analysis for routine epidemiological surveillance owing to its ability to test one specimen for several infections...
April 5, 2017: Virology Journal
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