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Infectious bronchitis virus

C A Torres, V Listorti, C Lupini, G Franzo, M Drigo, E Catelli, P E Brandão, M Cecchinato
In view of the restricted knowledge on the diversity of coronaviruses in poultry other than chicken, this study aimed to investigate the genetic diversity of coronaviruses in quail, pheasant, and partridge from two regions of Northern Italy. To this end, pools of tracheal and cloacal swabs from European quail (Coturnix Coturnix) and intestinal tract from pheasants (Phasianus Colchicus) and partridge (Perdix Perdix) flocks, with or without enteric signs, were collected during 2015. Avian coronavirus (Gammacoronavirus) was detected in quail not vaccinated against Infectious Bronchitis Virus (IBV) and in pheasants vaccinated with an IBV Massachusetts serotype...
October 12, 2016: Poultry Science
Paulo E Brandão, Giselle R R Ayres, Carolina A Torres, Laura Y B Villarreal, Aline S Hora, Sueli A Taniwaki
Avian coronavirus is the causative agent of infectious bronchitis in chickens, leading to multisystemic disease that might be controlled if adequate vaccine strains are used. This paper reports the first complete genome sequence of a Brazil type of this virus (27,615 nucleotides [nt]) isolated from the kidneys of a chicken.
October 13, 2016: Genome Announcements
Nancy L Sinai, Peter S Coates, Katelyn M Andrle, Chad Jefferis, C Gabriel Sentíes-Cué, Maurice E Pitesky
To better understand the potential avian diseases in Greater Sage-grouse ( Centrocercus urophasianus ) in the Great Basin in Nevada, we collected 31 blood samples March-April 2014 and tested for antibodies to eight viruses and two bacteria. Specifically, sera were tested for antibodies to avian leukosis virus type A, B, and J (ALV-A, ALV-B, and ALV-J, respectively), infectious bursal disease virus, infectious bronchitis virus, reticuloendothelial virus, avian influenza virus (AIV), West Nile virus, Pasteurella multocida (PM), and Salmonella enterica serovar Pullorum...
October 5, 2016: Journal of Wildlife Diseases
Giovanni Franzo, Claudia Maria Tucciarone, Angela Blanco, Miquel Nofrarías, Mar Biarnés, Marti Cortey, Natàlia Majó, Elena Catelli, Mattia Cecchinato
The extreme variability and rapid evolution of Infectious bronchitis virus (IBV) has always represented the key challenge for its control because of the limited cross-protection among different strains. Several experimental trials have proven a broadening of the protection spectrum when animals are vaccinated with multiple genotypes. Nevertheless, the conditions of vaccine administration in field are so different that the generalization of experimental results is, at least, questionable. In the present study a large scale epidemiological-phylodynamic approach was used to reconstruct the demographic history of the major field genotype (i...
September 23, 2016: Vaccine
Faruku Bande, Siti Suri Arshad, Mohd Hair Bejo, Saeid Kadkhodaei, Abdul Rahman Omar
Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, YTSNETTDVTS(175-185), was identified in M41 IBV strains while three such epitopes types namely, VSNASPNSGGVD(279-290), HPKCNFRPENI(328-338), and NETNNAGSVSDCTAGT(54-69), were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M41 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96-100% of the total M41 strains analysed...
2016: Advances in Bioinformatics
Lei Tan, Yuqiang Zhang, Fang Liu, Yanmei Yuan, Yuan Zhan, Yingjie Sun, Xusheng Qiu, Chunchun Meng, Cuiping Song, Chan Ding
Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In the present study, we developed IBV S1 glycoprotein poly-epitope-based DNA vaccine pV-S1B+S1T consisting of SH1208 and Holte strain BF2-restricted T cell epitopes and Australian T strain dominant B cell neutralization epitopes. Specific pathogen-free chickens were vaccinated with pV-S1B+S1T and control plasmids twice to elicit strong humoral and cellular immune response, as indicated by viral neutralization titers and results of CD8(+) T cell proliferation assays...
October 17, 2016: Vaccine
Ambalika Batra, Helena J Maier, Mark S Fife
Infectious bronchitis virus (IBV) causes infectious bronchitis in poultry, a respiratory disease that is a source of major economic loss to the poultry industry. Detection and the study of the molecular pathogenesis of the virus often involves the use of real-time quantitative PCR assays (qPCR). To account for error within the experiments, the levels of target gene transcription are normalised to that of suitable reference genes. Despite publication of the MIQE guidelines in 2009, single un-tested reference genes are often used for normalization of qPCR assays in avian research studies...
September 13, 2016: Avian Pathology: Journal of the W.V.P.A
L Pohjola, N Tammiranta, C Ek-Kommonen, T Soveri, M L Hänninen, M Fredriksson-Ahomaa, A Huovilainen
Backyard poultry is regaining popularity in Europe and increased interest in the health and management of non-commercial farms has resulted. Furthermore, commercial poultry farm owners have become concerned about the risk represented by contagious animal diseases that nearby backyard poultry could transmit. Fifty-one voluntary backyard chicken farms were visited between October 2012 and January 2013. Blood samples and individual cloacal swabs were collected from 457 chickens. In 44 farms (86%), one or more of the tested chickens had antibodies against avian encephalomyelitis and chicken infectious anemia viruses, 24 farms (47%) had chickens seropositive for infectious bronchitis virus, 10 farms (20%) had chickens seropositive for infectious bursal disease virus, six farms (12%) had chickens seropositive for infectious laryngotracheitis virus and two farms (5...
September 13, 2016: Avian Pathology: Journal of the W.V.P.A
Christina Leyson, Monique França, Mark Jackwood, Brian Jordan
Sequencing avian infectious bronchitis virus spike genes re-isolated from vaccinated chicks revealed that many sequence changes are found on the S1 spike gene. In the ArkDPI strain, Y43H and ∆344 are the two most common changes observed. This study aims to examine the roles of Y43H and ∆344 in selection in vivo. Using recombinant ArkDPI S1 proteins, we conducted binding assays on chicken tracheas and embryonic chorioallantoic membrane (CAM). Protein histochemistry showed that the Y43H change allows for enhanced binding to trachea, whereas the ArkDPI S1 spike with H43 alone was able to bind CAM...
November 2016: Virology
C A Torres, A S Hora, P O Tonietti, S A Taniwaki, M Cecchinato, L Y B Villarreal, P E Brandão
This paper expands on a previous report about coronaviruses in quail. After surveillance carried out in 2009 and 2010, some farmers started vaccinating quail with the Massachusetts avian infectious bronchitis virus serotype. The samples for this study were collected in 2013 from São Paulo state in southeastern Brazil. Pools of trachea, lungs, reproductive tract, kidneys, and enteric contents from quail and laying hens kept in the same farms and from quail-only farms as well as from both healthy birds and those showing infectious bronchitis-like symptoms were sampled in this study...
September 2016: Avian Diseases
Huseyin Yilmaz, Eda Altan, Utku Y Cizmecigil, Aydin Gurel, Gulay Yuzbasioglu Ozturk, Ozge Erdogan Bamac, Ozge Aydin, Paul Britton, Isabella Monne, Burhan Cetinkaya, Kenton L Morgan, Bonto Faburay, Juergen A Richt, Nuri Turan
The avian coronavirus infectious bronchitis virus (AvCoV-IBV) is recognized as an important global pathogen because new variants are a continuous threat to the poultry industry worldwide. This study investigates the genetic origin and diversity of AvCoV-IBV by analysis of the S1 sequence derived from 49 broiler flocks and 14 layer flocks in different regions of Turkey. AvCoV-IBV RNA was detected in 41 (83.6%) broiler flocks and nine (64.2%) of the layer flocks by TaqMan real-time RT-PCR. In addition, AvCoV-IBV RNA was detected in the tracheas 27/30 (90%), lungs 31/49 (62...
September 2016: Avian Diseases
Ali Zanaty, Mahmoud M Naguib, Mohamed H El-Husseiny, Wesam Mady, Naglaa Hagag, Abdel-Satar Arafa
Infectious bronchitis virus (IBV) continues to circulate worldwide, with a significant impact on the poultry industry and affecting both vaccinated and unvaccinated flocks. Several studies have focused on the hypervariable regions (HVRs) of the spike gene (S1); however, genetic and bioinformatics studies of the whole S1 gene are limited. In this study, the whole S1 gene of five Egyptian IBVs was genetically analyzed. Phylogenetic analysis revealed that the Egyptian IBVs are clustered within two distinct groups: the classic group resembling the GI-1 genotype (vaccine strains) and the variant group (field strains) of the GI-23 genotype...
December 2016: Archives of Virology
Qi Zhong, Yan-Xin Hu, Ji-Hui Jin, Ye Zhao, Jing Zhao, Guo-Zhong Zhang
Avian infectious bronchitis is an economically important poultry disease caused by avian infectious bronchitis virus (IBV). IBV isolate YN is a virulent strain, which is genetically similar to most of the prevalent strains in China. In this study, 21-day-old commercial laying hens were infected with IBV strain YN. The damaging effects of the virus on the reproductive organs were evaluated with clinical observations, gross autopsy and histopathological examinations during the 100-day monitoring period post infection...
September 25, 2016: Veterinary Microbiology
M S Rehman, A Mahmud, S Mehmood, T N Pasha, J Hussain, M T Khan
A study was conducted to investigate the effects of 3 different rearing systems [free range ( FR: ), semi-intensive ( SI: ), and confinement ( CF: )] on blood biochemical profile and immune response in 4 varieties of Aseel chicken [Lakha ( LK: ), Mushki ( MS: ), Peshawari ( PW: ) and Sindhi ( SN: )] for 10 wk duration (7 to 16 wk). At the age of 6 wk, in total, 180 cockerels were assigned to 12 treatment groups, 3 (rearing system) × 4 (Aseel chicken variety) factorial arrangement in 7 randomized complete blocks, replicated 3 times with 5 birds in each replicate (45 birds of each variety; 60 birds in each rearing system; 36 total replicates)...
September 2, 2016: Poultry Science
Md Rashedul Islam, Dave B Oomah, Moussa S Diarra
Studies on effects of cranberry products in animals, especially in chickens, are very scarce or even lacking. This study investigated the immunomodulatory effect of high molecular weight non-dialyzable materials (NDMs) of cranberry extract. The in vitro antioxidant and anti-inflammatory activities were investigated. The ability of NDMs (0, 1, 2, or 4 mg/mL) to enhance phagocytic activities was also evaluated using chicken heterophils (CHEs) against Staphylococcus aureus Furthermore, a broiler model was used to determine the effect of NDMs on the humoral immune response...
September 1, 2016: Poultry Science
Claiton Gonçalves Pereira, Giuliana Loreto Saraiva, Pedro Marcus Pereira Vidigal, Juliana Lopes Rangel Fietto, Gustavo Costa Bressan, Maria Aparecida Scatamburlo Moreira, Márcia Rogéria de Almeida, Abelardo Silva Júnior
On the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious bronchitis (IB). In the clinical disease stage, the Ma5 vaccine strain was detected in the trachea, lungs, and small intestine of the chicks, while IBV variants were detected in the bursa of Fabricius and kidneys...
December 2016: Archives of Virology
R A Leghari, B Fan, H Wang, J Bai, L Zhang, S H Abro, P Jiang
Infectious bronchitis virus (IBV) produces infectious bronchitis (IB) disease in poultry worldwide. In spite of proper vaccinations against the IBV, new IBV strains are continually emerging worldwide. In this study, a new highly virulent nephropathogenic IBV strain named CK/CH/XDC-2/2013 was identified from a vaccinated flock with clinical signs of IB in the Jiangsu province of China. The full-length genome sequence of the isolate was 27,714 nucleotides long, and the genome was organized similarly to classical IBV strains...
August 26, 2016: Poultry Science
Ali Zanaty, Abdel-Satar Arafa, Naglaa Hagag, Magdy El-Kady
AIM: To characterize the circulating infectious bronchitis virus (IBV) strains in Egypt depending on the sequence of the spike-1 (S1) gene [hypervariable region-3 (HVR-3)] and to study the pathotypic features of these strains. METHODS: In this work, twenty flocks were sampled for IBV detection using RRT-PCR and isolation of IBV in specific pathogen free (SPF) chicks during the period from 2010 to 2015. Partial sequencing and phylogenetic analysis of 400 bp representing the HVR-3 of the S1 gene was conducted...
August 12, 2016: World Journal of Virology
Nacira Laamiri, Pia Fällgren, Siamak Zohari, Jaouher Ben Ali, Abdeljelil Ghram, Mikael Leijon, Issam Hmila
A novel oligonucleotide suspension microarray (Luminex® microsphere system) is developed for rapid diagnosis of avian respiratory viruses of major clinical importance. This test was optimized and validated with 70 clinical samples. The developed tool was accurate for high throughput detection and differentiation of the most important avian respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV) in single and mixed viral infections...
August 24, 2016: Journal of Clinical Microbiology
Yingshun Zhou, Xin Yang, Hongning Wang, Fanya Zeng, Zhikun Zhang, Anyun Zhang, Hui Liu
Avian infectious bronchitis virus is an important pathogen in poultry worldwide. Vaccination is the only effective way to prevent and control IBV infection. H120, one of the safest vaccine strains, which has been used worldwide as a primary vaccine, cannot adapt to passaged cells, which severely restricts the quality of the vaccine. Based on the reverse genetics of our previous research work, we constructed the recombinant R-H120-Beaudette-p65(S) strain by replacing the complete spike gene of H120 with the corresponding spike gene from the Beaudette p65 strain...
November 2016: Archives of Virology
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