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Infectious bronchitis virus

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https://www.readbyqxmd.com/read/28081264/the-important-role-of-lipid-raft-mediated-attachment-in-the-infection-of-cultured-cells-by-coronavirus-infectious-bronchitis-virus-beaudette-strain
#1
Huichen Guo, Mei Huang, Quan Yuan, Yanquan Wei, Yuan Gao, Lejiao Mao, Lingjun Gu, Yong Wah Tan, Yanxin Zhong, Dingxiang Liu, Shiqi Sun
Lipid raft is an important element for the cellular entry of some viruses, including coronavirus infectious bronchitis virus (IBV). However, the exact role of lipid rafts in the cellular membrane during the entry of IBV into host cells is still unknown. In this study, we biochemically fractionated IBV-infected cells via sucrose density gradient centrifugation after depleting plasma membrane cholesterol with methyl-β-cyclodextrin or Mevastatin. Our results demonstrated that unlike IBV non-structural proteins, IBV structural proteins co-localized with lipid raft marker caveolin-1...
2017: PloS One
https://www.readbyqxmd.com/read/28062013/origin-and-evolution-of-lx4-genotype-infectious-bronchitis-coronavirus-in-china
#2
Wenjun Zhao, Mengying Gao, Qianqian Xu, Yang Xu, Yan Zhao, Yuqiu Chen, Tingting Zhang, Qiuling Wang, Zongxi Han, Huixin Li, Lingfeng Chen, Shuling Liang, Yuhao Shao, Shengwang Liu
We investigated the genomic characteristics of 110 LX4 genotype strains of infectious bronchitis viruses (IBVs) isolated between 1995 and 2005 in China. The genome of these IBVs varies in size from 27596bp to 27790bp. Most IBV strains have the typical genomic organization of other gamacoronaviruses, however, two strains lacked 3a and 5b genes as a result of a nucleotide change within the start codon in the 3a or 5b genes. Analysis of our 110 viruses revealed that recombination events may be responsible for the emergence of the LX4 genotype with different topologies...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28062000/identification-and-molecular-characterization-of-a-novel-serotype-infectious-bronchitis-virus-gi-28-in-china
#3
Yuqiu Chen, Lei Jiang, Wenjun Zhao, Liangliang Liu, Yan Zhao, Yuhao Shao, Huixin Li, Zongxi Han, Shengwang Liu
Avian infectious bronchitis coronavirus (IBV) is a major poultry pathogen. A characteristic feature of IBV is the occurrence of many different strains belonging to different serotypes, which renders complete control of the disease by vaccination a challenging task due to the poor cross-protection between different serotypes. In this study, based on the results of S1 sequence analysis and virus cross-neutralization tests, IBV strain ck/CH/LGX/111119 was found to be genetically and antigenically different from other known IBV types, representing not only a novel genotype, but also a novel serotype (designated as GI-28)...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/27974570/channel-inactivating-mutations-and-their-revertant-mutants-in-the-envelope-protein-of-the-infectious-bronchitis-virus
#4
Janet To, Wahyu Surya, To Sing Fung, Yan Li, Carmina Verdià-Bàguena, Maria Queralt-Martin, Vicente M Aguilella, Ding Xiang Liu, Jaume Torres
: It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E protein channel activity. In particular, V25F was rapidly compensated by changes at multiple V25F-facing TMD residues located on a neighboring monomer, consistent with a recovery of oligomerization...
December 14, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27938680/full-genome-analysis-of-australian-infectious-bronchitis-viruses-suggests-frequent-recombination-events-between-vaccine-strains-and-multiple-phylogenetically-distant-avian-coronaviruses-of-unknown-origin
#5
José A Quinteros, Sang-Won Lee, Philip F Markham, Amir H Noormohammadi, Carol A Hartley, Alistair R Legione, Mauricio J C Coppo, Paola K Vaz, Glenn F Browning
Australian strains of infectious bronchitis virus (IBV) have been evolving independently for many years, with control achieved by vaccination with local attenuated strains. Previous studies have documented the emergence of recombinants over the last 20 years, with the most recent one, Ck/Aus/N1/08, detected in 2008. These recombinants did not appear to be controlled by the vaccines currently in use. In this study we sequenced the complete genomes of three emergent Australian strains of IBV (IBV/Ck/Aus/N1/88, IBV/Ck/Aus/N1/03 and IBV/Ck/Aus/N1/08) and a previously incompletely characterised vaccine strain, IBV/Ck/Aus/Armidale, and compared them to the genome of the vaccine strain VicS...
December 25, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27928919/pathogenicity-characteristics-of-an-iranian-variant-2-is-1494-like-infectious-bronchitis-virus-in-experimentally-infected-spf-chickens
#6
H Najafi, A Ghalyanchi Langeroudi, M Hashemzadeh, O Madadgar, V Karimi, R K Farahani, H Abdollahi, H Maghsoudsloo, P Seifouri
Avian infectious bronchitis (IB) is a major cause of economic loss to the poultry industry. IB virus primarily affects respiratory tract, but strains differ in their tropism for such other target organs as kidneys and alimentary tract. The objective of this study was to estimate the pathogenicity of an Iranian IB virus (IBV) variant (variant-2) which is one of the most prevalent isolates circulating in Iranian poultry farms. SPF chickens were intranasally inoculated with 104 EID50/0.1 ml of the virus. Sera, fecal swabs, and different tissue samples were collected on different days post infection...
2016: Acta Virologica
https://www.readbyqxmd.com/read/27924415/detection-of-infectious-bronchitis-virus-793b-avian-metapneumovirus-mycoplasma-gallisepticum-and-mycoplasma-synoviae-in-poultry-in-ethiopia
#7
S Hutton, J Bettridge, R Christley, T Habte, K Ganapathy
A survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the Ethiopian Institute of Agricultural Research (EIAR), located in Debre Zeit, Ethiopia. Oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. A combination of serological and molecular methods was used for detection of pathogens. For the first time in Ethiopia, we report the detection of variant infectious bronchitis virus (793B genotype), avian metapneumovirus subtype B and Mycoplasma synoviae in poultry...
December 6, 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27917677/infectious-bronchitis-vaccine-virus-detection-and-part-s1-genetic-variation-following-single-or-dual-inoculation-in-broiler-chicks
#8
Christopher Ball, Faez Awad, Sally Hutton, Anne Forrester, Matthew Baylis, Kannan Ganapathy
An investigation was undertaken of the extent of genetic variation occurring within infectious bronchitis virus (IBV) vaccine strains following vaccination of day-old broiler chicks. Chicks were divided into seven groups, with two groups receiving single Massachusetts (Mass) vaccinations and the other four were inoculated with combinations of different IBV serotypes; Mass, 793B, D274, and Arkansas (Ark). The remaining group was maintained as an unvaccinated control. Following vaccination, swabs and tissues collected at intervals were pooled and RNA was extracted for detection of IBV by reverse transcription polymerase chain reaction (RT PCR)...
December 5, 2016: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/27902312/genetic-mutations-in-live-infectious-bronchitis-vaccine-viruses-following-single-or-dual-in-vitro-infection-of-tracheal-organ-cultures
#9
Christopher Ball, Sarah Bennett, Anne Forrester, Kannan Ganapathy
Despite regular co-vaccination of two different strains of live infectious bronchitis vaccine viruses, little is known about possible mutations in these viruses following vaccination. As an alternative to chicks, this study used an in vitro infection model to identify single-nucleotide polymorphisms (SNPs) within the part-S1 gene of two live infectious bronchitis virus vaccine strains (793B and Massachusetts) following single or dual inoculation onto tracheal organ cultures. Results indicate that viral titres reduced over the duration of the study; conversely, the amount of detected infectious bronchitis virus genome increased...
December 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27876864/identification-of-an-infectious-bronchitis-coronavirus-strain-exhibiting-a-classical-genotype-but-altered-antigenicity-pathogenicity-and-innate-immunity-profile
#10
Shu-Yi Lin, Yao-Tsun Li, You-Ting Chen, Ting-Chih Chen, Che-Ming J Hu, Hui-Wen Chen
Avian coronavirus infectious bronchitis virus (IBV) poses economic threat to the poultry industry worldwide. Pathogenic IBV 3575/08 was isolated from broilers vaccinated with the attenuated viral vaccine derived from a Taiwan strain 2575/98. In this study, extensive investigations were conducted on the genome sequences, antigenicity, pathogenicity, and host immune responses of several IBV strains in specific-pathogen-free chickens. Sequence analyses revealed that 3575/08 and 2575/98 shared high homology in their structural genes, but not in non-structural accessory proteins such as 3a, 3b and 5b...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27836775/insights-from-molecular-structure-predictions-of-the-infectious-bronchitis-virus-s1-spike-glycoprotein
#11
Christina Lora M Leyson, Brian J Jordan, Mark W Jackwood
Infectious bronchitis virus is an important respiratory pathogen in chickens. The IBV S1 spike is a viral structural protein that is responsible for attachment to host receptors and is a major target for neutralizing antibodies. To date, there is no experimentally determined structure for the IBV S1 spike. In this study, we sought to find a predicted tertiary structure for IBV S1 using I-TASSER, which is an automated homology modeling platform. We found that the predicted structures obtained were robust and consistent with experimental data...
December 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27803698/pathogenicity-of-a-tw-like-strain-of-infectious-bronchitis-virus-and-evaluation-of-the-protection-induced-against-it-by-a-qx-like-strain
#12
Shi-Hong Yan, Yang Chen, Jing Zhao, Gang Xu, Ye Zhao, Guo-Zhong Zhang
Avian infectious bronchitis, a highly contagious disease caused by avian infectious bronchitis virus (IBV), is of considerable economic importance to the poultry industry. New IBV TW-like strains have increasingly emerged in China in recent years; hence, evaluating their pathogenicity and developing a specific vaccine to guard against their potential threat to the poultry industry is important. Here, we examined the pathogenicity of a TW-like IBV strain (GD), and evaluated the protective efficacy of the QX-like strain (JS) against GD in challenge infections in chickens...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27796116/health-evaluation-of-african-penguins-i-spheniscus-demersus-i-in-southern-africa
#13
Nola J Parsons, Tertius A Gous, Adam M Schaefer, Ralph E T Vanstreels
The African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a rehabilitation centre...
September 20, 2016: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/27793730/full-genome-sequence-analysis-of-a-newly-emerged-qx-like-infectious-bronchitis-virus-from-sudan-reveals-distinct-spots-of-recombination
#14
Mahmoud M Naguib, Dirk Höper, Abdel-Satar Arafa, Ahmed M Setta, Mohamed Abed, Isabella Monne, Martin Beer, Timm C Harder
Infectious bronchitis virus (IBV) infection continues to cause economically important diseases in poultry while different geno- and serotypes continue to circulate globally. Two infectious bronchitis viruses (IBV) were isolated from chickens with respiratory disease in Sudan. Sequence analysis of the hypervariable regions of the S1 gene revealed a close relation to the QX-like genotype which has not been detected in Sudan before. Whole genome analysis of IBV/Ck/Sudan/AR251-15/2014 isolate by next generation sequencing revealed a genome size of 27,646 nucleotides harbouring 13 open reading frames: 5'-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-3'...
December 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27738120/gamma-and-deltacoronaviruses-in-quail-and-pheasants-from-northern-italy
#15
C A Torres, V Listorti, C Lupini, G Franzo, M Drigo, E Catelli, P E Brandão, M Cecchinato
In view of the restricted knowledge on the diversity of coronaviruses in poultry other than chicken, this study aimed to investigate the genetic diversity of coronaviruses in quail, pheasant, and partridge from two regions of Northern Italy. To this end, pools of tracheal and cloacal swabs from European quail (Coturnix Coturnix) and intestinal tract from pheasants (Phasianus Colchicus) and partridge (Perdix Perdix) flocks, with or without enteric signs, were collected during 2015. Avian coronavirus (Gammacoronavirus) was detected in quail not vaccinated against Infectious Bronchitis Virus (IBV) and in pheasants vaccinated with an IBV Massachusetts serotype...
October 12, 2016: Poultry Science
https://www.readbyqxmd.com/read/27738043/complete-genome-sequence-of-a-brazil-type-avian-coronavirus-detected-in-a-chicken
#16
Paulo E Brandão, Giselle R R Ayres, Carolina A Torres, Laura Y B Villarreal, Aline S Hora, Sueli A Taniwaki
Avian coronavirus is the causative agent of infectious bronchitis in chickens, leading to multisystemic disease that might be controlled if adequate vaccine strains are used. This paper reports the first complete genome sequence of a Brazil type of this virus (27,615 nucleotides [nt]) isolated from the kidneys of a chicken.
October 13, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27705104/a-serosurvey-of-greater-sage-grouse-centrocercus-urophasianus-in-nevada-usa
#17
Nancy L Sinai, Peter S Coates, Katelyn M Andrle, Chad Jefferis, C Gabriel Sentíes-Cué, Maurice E Pitesky
To better understand the potential avian diseases in Greater Sage-grouse ( Centrocercus urophasianus ) in the Great Basin in Nevada, US, we collected 31 blood samples March-April 2014 and tested for antibodies to eight viruses and two bacteria. Specifically, sera were tested for antibodies to avian leukosis virus type A, B, and J (ALV-A, ALV-B, and ALV-J, respectively), infectious bursal disease virus, infectious bronchitis virus, reticuloendothelial virus, avian influenza virus (AIV), West Nile virus, Pasteurella multocida (PM), and Salmonella enterica serovar Pullorum...
January 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/27670071/effect-of-different-vaccination-strategies-on-ibv-qx-population-dynamics-and-clinical-outbreaks
#18
Giovanni Franzo, Claudia Maria Tucciarone, Angela Blanco, Miquel Nofrarías, Mar Biarnés, Marti Cortey, Natàlia Majó, Elena Catelli, Mattia Cecchinato
The extreme variability and rapid evolution of Infectious bronchitis virus (IBV) has always represented the key challenge for its control because of the limited cross-protection among different strains. Several experimental trials have proven a broadening of the protection spectrum when animals are vaccinated with multiple genotypes. Nevertheless, the conditions of vaccine administration in field are so different that the generalization of experimental results is, at least, questionable. In the present study a large scale epidemiological-phylodynamic approach was used to reconstruct the demographic history of the major field genotype (i...
September 23, 2016: Vaccine
https://www.readbyqxmd.com/read/27667997/prediction-and-in-silico-identification-of-novel-b-cells-and-t-cells-epitopes-in-the-s1-spike-glycoprotein-of-m41-and-cr88-793-b-infectious-bronchitis-virus-serotypes-for-application-in-peptide-vaccines
#19
Faruku Bande, Siti Suri Arshad, Mohd Hair Bejo, Saeid Kadkhodaei, Abdul Rahman Omar
Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, YTSNETTDVTS(175-185), was identified in M41 IBV strains while three such epitopes types namely, VSNASPNSGGVD(279-290), HPKCNFRPENI(328-338), and NETNNAGSVSDCTAGT(54-69), were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M41 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96-100% of the total M41 strains analysed...
2016: Advances in Bioinformatics
https://www.readbyqxmd.com/read/27665355/infectious-bronchitis-virus-poly-epitope-based-vaccine-protects-chickens-from-acute-infection
#20
Lei Tan, Yuqiang Zhang, Fang Liu, Yanmei Yuan, Yuan Zhan, Yingjie Sun, Xusheng Qiu, Chunchun Meng, Cuiping Song, Chan Ding
Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In the present study, we developed IBV S1 glycoprotein poly-epitope-based DNA vaccine pV-S1B+S1T consisting of SH1208 and Holte strain BF2-restricted T cell epitopes and Australian T strain dominant B cell neutralization epitopes. Specific pathogen-free chickens were vaccinated with pV-S1B+S1T and control plasmids twice to elicit strong humoral and cellular immune response, as indicated by viral neutralization titers and results of CD8(+) T cell proliferation assays...
October 17, 2016: Vaccine
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