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https://www.readbyqxmd.com/read/29220051/improving-extraction-and-post-purification-concentration-of-membrane-proteins
#1
Hasin Feroz, HyeYoung Kwon, Jing Peng, Hyeonji Oh, Bryan Ferlez, Carol S Baker, John H Golbeck, Guillermo C Bazan, Andrew L Zydney, Manish Kumar
Membrane proteins (MPs), despite being critically important drug targets for the pharmaceutical industry, are difficult to study due to challenges in obtaining high yields of functional protein. Most current extraction efforts use specialized non-ionic detergents to solubilize and stabilize MPs, with MPs being concentrated by ultrafiltration (UF). However, many detergents are retained during the UF step, which can destabilize MPs and/or interfere with their characterization. Here, we studied the influence of detergent selection on the extraction and UF-based concentration of biomedically-relevant MPs, the light-driven sodium and chloride transporters, KR2 and halorhodopsin (pHR) which are also model proteins for more complex mammalian rhodopsins...
December 8, 2017: Analyst
https://www.readbyqxmd.com/read/29204499/variance-in-predicted-cup-size-by-2-dimensional-vs-3-dimensional-computerized-tomography-based-templating-in-primary-total-hip%C3%A2-arthroplasty
#2
Feroz A Osmani, Savyasachi Thakkar, Austin Ramme, Ameer Elbuluk, Paul Wojack, Jonathan M Vigdorchik
Background: Preoperative total hip arthroplasty templating can be performed with radiographs using acetate prints, digital viewing software, or with computed tomography (CT) images. Our hypothesis is that 3D templating is more precise and accurate with cup size prediction as compared to 2D templating with acetate prints and digital templating software. Methods: Data collected from 45 patients undergoing robotic-assisted total hip arthroplasty compared cup sizes templated on acetate prints and OrthoView software to MAKOplasty software that uses CT scan...
December 2017: Arthroplasty Today
https://www.readbyqxmd.com/read/29198633/intraoperative-echocardiographic-assessment-of-prosthetic-valves-a-practical-approach
#3
REVIEW
Faraz Mahmood, Robina Matyal, Feroze Mahmood, Richard D Sheu, Ruby Feng, Kamal R Khabbaz
No abstract text is available yet for this article.
November 2, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29185439/a-survey-on-the-pattern-of-dermoscopy-use-among-dermatologists-in-india
#4
Feroze Kaliyadan, Karalikkattil T Ashique, Soumya Jagadeesan
INTRODUCTION AND BACKGROUND: Dermoscopy is being increasingly used for improving dermatological diagnosis. Use of dermoscopy in the early recognition of skin malignancies, especially melanoma, is well established. Of late, its use in general clinical dermatology is growing with the recognition of new and specific patterns in conditions such as hair disorders, inflammatory disorders, and infections/infestations. This cross-sectional survey aims to assess the common patterns of dermoscopy use by Indian dermatologists...
November 28, 2017: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/29171574/methanol-poisoning-27-years-experience-at-a-tertiary-care-hospital
#5
Fareed Ahmed, Nadeem Ullah Khan, Noman Ali, Asher Feroze
Methanol toxicity can result in serious morbidity and mortality without timely diagnosis and treatment. Many cases of methanol poisoning outbreaks have been noted in our population but no study has been performed to estimate methanol exposure and its outcomes and complications. A retrospective study was conducted to review all the cases of methanol poisoning admitted from January 1988 to December 2015 at the Aga Khan University Hospital. A total of 35 methanol poisoning cases were reported. All the patients were male, and the mean age was 36...
November 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/29154897/identification-of-ghost-artifact-using-texture-analysis-in-pediatric-spinal-cord-diffusion-tensor-images
#6
Mahdi Alizadeh, Chris J Conklin, Devon M Middleton, Pallav Shah, Sona Saksena, Laura Krisa, Jürgen Finsterbusch, Scott H Faro, M J Mulcahey, Feroze B Mohamed
PURPOSE: Ghost artifacts are a major contributor to degradation of spinal cord diffusion tensor images. A multi-stage post-processing pipeline was designed, implemented and validated to automatically remove ghost artifacts arising from reduced field of view diffusion tensor imaging (DTI) of the pediatric spinal cord. METHOD: A total of 12 pediatric subjects including 7 healthy subjects (mean age=11.34years) with no evidence of spinal cord injury or pathology and 5 patients (mean age=10...
November 15, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/29123604/factors-behind-not-using-child-restrain-t-among-hospital-employees-and-general-population-a-case-control-study
#7
Emad Siddiqui, Badar Afzal, Ghazala Kazi, Asher Feroz, Rubaba Naeem, Tarab Mansoor, Ahreen Allana, Saif Siddiqui, Zain Siddiqui
BACKGROUND: Motor vehicle crash (MVC) related injury has been identified as a major public health concern. Child restrain (CR) seat belts can minimize the mortality and morbidity from MVC. The use for seat belts is substantially low in developing countries like Pakistan even though its use has been shown to decrease morbidity and mortality by a significant extent. METHODS: This was a case control study with cases from the general population (GP) and controls from the Aga Khan University (AKU) employees in a 3:1 ratio...
2017: World Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29115992/role-of-mhealth-applications-for-improving-antenatal-and-postnatal-care-in-low-and-middle-income-countries-a-systematic-review
#8
Anam Feroz, Shagufta Perveen, Wafa Aftab
BACKGROUND: From 1990 to 2015, the number of maternal deaths globally has dropped by 43%. Despite this, progress in attaining MDG 5 is not remarkable in LMICs. Only 52% of pregnant women in LMICs obtain WHO recommended minimum of four antenatal consultations and the coverage of postnatal care is relatively poor. In recent years, the increased cellphone penetration has brought the potential for mHealth to improve preventive maternal healthcare services. The objective of this review is to assess the effectiveness of mHealth solutions on a range of maternal health outcomes by categorizing the interventions according to the types of mHealth applications...
November 7, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/29107536/the-unfolded-protein-response-and-cell-fate-control
#9
REVIEW
Claudio Hetz, Feroz R Papa
The secretory capacity of a cell is constantly challenged by physiological demands and pathological perturbations. To adjust and match the protein-folding capacity of the endoplasmic reticulum (ER) to changing secretory needs, cells employ a dynamic intracellular signaling pathway known as the unfolded protein response (UPR). Homeostatic activation of the UPR enforces adaptive programs that modulate and augment key aspects of the entire secretory pathway, whereas maladaptive UPR outputs trigger apoptosis. Here, we discuss recent advances into how the UPR integrates information about the intensity and duration of ER stress stimuli in order to control cell fate...
October 25, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29100836/changes-in-tricuspid-annular-geometry-in-patients-with-functional-tricuspid-regurgitation
#10
Ting Hai, Yannis Amador, Feroze Mahmood, Jelliffe Jeganathan, Arash Khamooshian, Ziyad O Knio, Robina Matyal, Alina Nicoara, David C Liu, Venkatachalam Senthilnathan, Kamal R Khabbaz
OBJECTIVE: To determine whether the indices of tricuspid annular dynamics that signify irreversible tricuspid valvular remodeling can improve surgical decision making by helping to better identify patients with functional tricuspid regurgitation who could benefit from annuloplasty. DESIGN: Retrospective analysis study. SETTING: Tertiary hospital. PARTICIPANTS: A total number of 55 patients were selected, 18 with functional tricuspid valve (TV) regurgitation and 37 normal nonregurgitant TVs...
June 20, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29073810/reduced-field-of-view-diffusion-tensor-imaging-and-fiber-tractography-of-the-pediatric-cervical-and-thoracic-spinal-cord-injury
#11
Mahdi Alizadeh, Joshua Fisher, Sona Saksena, Yusra Sultan, Christopher Conklin, Devon Middleton, Jürgen Finsterbusch, Laura Krisa, Adam Flanders, Scott Faro, M J Mulcahey, Feroze Mohamed
The purpose of this study is to assess the utility and effectiveness of diffusion tensor imaging (DTI) and diffusion tensor tractography (DTT) of the entire pediatric cervical and thoracic spinal cord towards discrimination of typically developing (TD) controls and subjects with spinal cord injury (SCI). A total of 43 pediatric subjects including 23 TD subjects ranging in age from 6-16 years old and 20 subjects with SCI ranging in age from 7-16 years old were recruited and scanned using a 3.0T MR scanner. Reduced field of view (FOV) diffusion tensor images were acquired axially to cover the entire spinal cord across two slabs...
October 26, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29052102/changes-in-cerebellar-functional-connectivity-and-autonomic-regulation-in-cancer-patients-treated-with-the-neuro-emotional-technique-for-traumatic-stress-symptoms
#12
Daniel A Monti, Anna Tobia, Marie Stoner, Nancy Wintering, Michael Matthews, Chris J Conklin, Feroze B Mohamed, Inna Chervoneva, Andrew B Newberg
PURPOSE: A growing number of research studies have implicated the cerebellum in emotional processing and regulation, especially with regard to negative emotional memories. However, there currently are no studies showing functional changes in the cerebellum as a result of treatment for traumatic stress symptoms. The Neuro Emotional Technique (NET) is an intervention designed to help improve symptoms related to traumatic stress using an integrative approach that combines emotional, cognitive, and motor processing, with a particular focus on autonomic nervous system regulation...
October 19, 2017: Journal of Cancer Survivorship: Research and Practice
https://www.readbyqxmd.com/read/28969730/erythrodermic-psoriasis-and-hepatitis-c-infection-treated-with-pegylated-interferon-and-anti-tnf%C3%AE-%C3%AE-etanercept-therapy
#13
Humaira Talat, Zarnaz Wahid, Farheena Feroz, Madiha Sajid
Treatment of psoriasis and its variants with concomitant hepatitis C virus (HCV) infection is complex. The treatment options are limited because the immunosuppressive drugs used for managing severe psoriasis are mostly associated with end-organ damage particularly hepatotoxicity. On the other hand, Interferon therapy has the potential to exacerbate psoriasis and psoriatic arthropathy. There is emerging data regarding the use of etanercept, a TNFα inhibitor in such cases. Though its cost and availability limits its use; but when combined with Interferon therapy and ribavirin for HCV, it has shown good results...
September 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28965799/multifactorial-risk-index-for-prediction-of-intraoperative-blood-transfusion-in-endovascular-aneurysm-repair
#14
Eitezaz Mahmood, Robina Matyal, Ariel Mueller, Feroze Mahmood, Avery Tung, Mario Montealegre-Gallegos, Marc Schermerhorn, Sajid Shahul
BACKGROUND: In some institutions, the current blood ordering practice does not discriminate minimally invasive endovascular aneurysm repair (EVAR) from open procedures, with consequent increasing costs and likelihood of blood product wastage for EVARs. This limitation in practice can possibly be addressed with the development of a reliable prediction model for transfusion risk in EVAR patients. We used the American College of Surgeons National Surgical Quality Improvement Program (ACS NSQIP) database to create a model for prediction of intraoperative blood transfusion occurrence in patients undergoing EVAR...
September 28, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28962577/acute-maternal-oxidant-exposure-causes-susceptibility-of-the-fetal-brain-to-inflammation-and-oxidative-stress
#15
Feroz Akhtar, Christopher A Rouse, Gabriel Catano, Marcus Montalvo, Sarah L Ullevig, Reto Asmis, Kusum Kharbanda, Shivani K Maffi
BACKGROUND: Maternal exposure to environmental stressors poses a risk to fetal development. Oxidative stress (OS), microglia activation, and inflammation are three tightly linked mechanisms that emerge as a causal factor of neurodevelopmental anomalies associated with prenatal ethanol exposure. Antioxidants such as glutathione (GSH) and CuZnSOD are perturbed, and their manipulation provides evidence for neuroprotection. However, the cellular and molecular effects of GSH alteration in utero on fetal microglia activation and inflammation remain elusive...
September 30, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28961675/nasopharyngeal-pneumococcal-colonization-and-impact-of-a-single-dose-of-13-valent-pneumococcal-conjugate-vaccine-in-indian-children-with-hiv-and-their-unvaccinated-parents
#16
Bikas K Arya, Sangeeta Das Bhattacharya, Catherine G Sutcliffe, Feroze Ganaie, Arun Bhaskar, Subhasish Bhattacharyya, Swapan Kumar Niyogi, William J Moss, Samiran Panda, K L Ravikumar, Ranjan Saurav Das, Sutapa Mandal
BACKGROUND: HIV infection increases risk of invasive disease from Streptococcus pneumoniae. Pneumococcal conjugate vaccines (PCV) prevent invasive disease and acquisition of vaccine type (VT) pneumococcus in the nasopharynx. OBJECTIVE: To look at the safety and impact of one dose of PCV13 on acquisition of VT pneumococcal carriage in Indian children with HIV. METHOD: We conducted a cohort study in families of HIV-infected children (CLH) and families of HIV-uninfected children (HUC) in West Bengal...
September 27, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28935966/silent-sentence-completion-shows-superiority-localizing-wernicke-s-area-and-activation-patterns-of-distinct-language-paradigms-correlate-with-genomics-prospective-study
#17
Kamel El Salek, Islam S Hassan, Aikaterini Kotrotsou, Srishti Abrol, Scott H Faro, Feroze B Mohamed, Pascal O Zinn, Wei Wei, Nan Li, Ashok J Kumar, Jeffrey S Weinberg, Jeffrey S Wefel, Shelli R Kesler, Ho-Ling Anthony Liu, Ping Hou, R Jason Stafford, Sujit Prabhu, Raymond Sawaya, Rivka R Colen
Preoperative mapping of language areas using fMRI greatly depends on the paradigms used, as different tasks harness distinct capabilities to activate speech processing areas. In this study, we compared the ability of 3 covert speech paradigms: Silent Sentence Completion (SSC), category naming (CAT) and verbal fluency (FAS), in localizing the Wernicke's area and studied the association between genomic markers and functional activation. Fifteen right-handed healthy volunteers and 35 mixed-handed patients were included...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28917474/dermabrasion-in-earlobe-repair
#18
Karalikkattil T Ashique, Feroze Kaliyadan, Rinu Ruth George
No abstract text is available yet for this article.
October 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28902603/the-utility-of-conservative-treatment-modalities-in-the-management-of-osteonecrosis
#19
Feroz Osmani, Savyasachi Thakkar, Jonathan Vigdorchik
INTRODUCTION: Osteonecrosis is an ischemic pathologic process associated with a number of conditions affecting a range of age groups. The problem faced in the management of osteonecrosis is whether conservative treatment is a viable and effective option for patients. In this systematic review, we investigated the efficacy of various nonoperative treatment modalities for hip and knee osteonecrosis, including pharmacological management and biophysical modalities. METHODS: We identified 16 studies based on electronic searches through the PubMed, Embase, CINAHL Plus, and Cochrane databases from January 2001 to November 2015...
May 2017: Bulletin of the Hospital for Joint Diseases
https://www.readbyqxmd.com/read/28873411/preoperative-asymptomatic-leukocytosis-and-postoperative-outcome-in-cardiac-surgery-patients
#20
Eitezaz Mahmood, Ziyad O Knio, Feroze Mahmood, Rabia Amir, Sajid Shahul, Bilal Mahmood, Yanick Baribeau, Ariel Mueller, Robina Matyal
BACKGROUND: Despite showing a prognostic value in general surgical patients, preoperative asymptomatic elevated white blood cell (WBC) count is not considered a risk factor for cardiac surgery. Whereas there is sporadic evidence of its value as a preoperative risk marker, it has not been looked at methodically as a specific index of outcome during cardiac surgery. Using a national database we sought to determine the relationship between preoperative WBC count and postoperative outcome in cardiac surgical patients...
2017: PloS One
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