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https://www.readbyqxmd.com/read/28794865/recent-advances-in-acute-promyelocytic-leukaemia
#1
REVIEW
Chin-Hin Ng, Wee-Joo Chng
Acute promyelocytic leukaemia (APML) is a subtype of leukaemia arising from a distinct reciprocal translocation involving chromosomes 15 and 17, which results in the PML-RARA fusion gene. Over the past three decades, APML has been transformed from a highly fatal disease to a highly curable one. This drastic improvement is because of the introduction of a new treatment strategy with all-trans retinoic acid and, more recently, arsenic trioxide. The revolutionary treatment of APML has also paved the way for a new cancer treatment, which is genetically targeted therapy...
2017: F1000Research
https://www.readbyqxmd.com/read/28792633/evolutionarily-diverse-syp1-qa-snares-jointly-sustain-pollen-tube-growth-in-arabidopsis
#2
Daniel Slane, Ilka Reichardt, Farid El Kasmi, Martin Bayer, Gerd Jürgens
Intracellular membrane fusion is effected by SNARE proteins that reside on adjacent membranes and form bridging trans-SNARE complexes. Qa-SNARE members of the Arabidopsis SYP1 family are involved in membrane fusion at the plasma membrane or during cell plate formation. Three SYP1 family members have been classified as pollen-specific as inferred from gene expression profiling studies and two of them, SYP124 and SYP125, are confined to angiosperms. The SYP124 gene appears genetically unstable whereas its sister gene SYP125 shows essentially no variation among Arabidopsis accessions...
August 9, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28765554/non-peptide-guided-auto-secretion-of-recombinant-proteins-by-super-folder-green-fluorescent-protein-in-escherichia-coli
#3
Zhen Zhang, Rongxing Tang, Dewu Zhu, Wenfeng Wang, Li Yi, Lixin Ma
Protein secretion in Escherichia coli is usually led by a signal peptide that targets the protein to specific secretory pathways. In this study, we demonstrated that the superfolder green fluorescent protein (sfGFP) could be served as a non-signal peptide to guide protein auto-secretion in E. coli. This auto-secretion was characterized as a three-step process through the sub-cellular localization analysis: inner membrane trans-location followed by anchoring at outer membrane, and then being released into culture media...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28753465/engineering-an-auto-activated-r-protein-that-is-in-vivo-activated-by-a-viral-protease
#4
Phu-Tri Tran, Kristin Widyasari, Jee Yoon Park, Kook-Hyung Kim
Autonomous hypersensitive responses (self-HRs) are caused by constitutively active R proteins. In this study, we identified an auto-activated form of the R gene Pvr9 (autoPvr9); the auto-activation results from an amino acid substitution between its NBS and LRR domains. Self-HR was strongly reduced or completely inhibited by fusion of an extra peptide to the autoPvr9 N-terminal or C-terminal, respectively. When an NIa recognition site was placed between autoPvr9 and the extra peptide, the fusion construct could trigger an NIa-dependent HR...
July 25, 2017: Virology
https://www.readbyqxmd.com/read/28753226/golgi-%C3%AE-1-4-fucosyltransferase-of-arabidopsis-thaliana-partially-localizes-at-the-nuclear-envelope
#5
Stephan Rips, Manuel Frank, Annegret Elting, Jan Niklas Offenborn, Antje von Schaewen
We analyzed plant-derived α1,4-Fucosyltransferase (FucTc) homologs by reporter fusions and focused on representatives of the Brassicaceae and Solanaceae. Arabidopsis thaliana AtFucTc-GFP or tomato LeFucTc-GFP restored Lewis-a formation in a fuctc mutant, confirming functionality in the trans-Golgi. AtFucTc-GFP partly accumulated at the nuclear envelope (NE) not observed for other homologs or truncated AtFucTc lacking the N-terminus or catalytic domain. Analysis of At/LeFucTc-GFP swap constructs with exchanged cytosolic, transmembrane and stalk (CTS), or only the CT regions, revealed that sorting information resides in the membrane anchor...
July 28, 2017: Traffic
https://www.readbyqxmd.com/read/28752912/functional-analysis-of-the-cyclophilin-ppib-role-in-bacterial-cell-division
#6
Aggeliki Skagia, Chrysoula Zografou, Anastasia Venieraki, Costas Fasseas, Panagiotis Katinakis, Maria Dimou
Escherichia coli PpiB is a peptidyl-prolyl cis/trans isomerase (PPIase, EC: 5.2.1.8) with chaperone activity. Here, we show that the ΔppiB deletion strain and the PpiB over-expression wild-type strain are both characterized by defects in cell division involving milder or severe cell filamentation, respectively. Using various PpiB mutants, we show that the PPIase activity of PpiB is necessary for the observed cell filamentation, whereas other structural features apart from the active site are also important for this phenotype...
July 28, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28752203/treatment-of-atlanto-axial-subluxation-secondary-to-rheumatoid-arthritis-by-short-segment-stabilization-with-polyaxial-screws
#7
Petr Vanek, Ondrej Bradac, Patricia de Lacy, Karel Pavelka, Martina Votavova, Vladimir Benes
OBJECTIVE: The main aim of this study was to analyse the compex clinical and radiographic findings in a group of RA patients with atlanto-axial slip (AAS) treated with free-hand short C1 lateral mass and C2 trans-pedicular screw fixation. The surgical technique used and the pathology treated were the same in all patients, producing a very homogeneous cohort of patients This allowed the study and measurement of radiographic parameters and fusion process. METHODS: Twenty-nine patients (21 female, 8 male, mean age 54...
July 27, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28751315/scyl2-genes-are-involved-in-clathrin-mediated-vesicle-trafficking-and-essential-for-plant-growth
#8
Ji-Yul Jung, Dong Wook Lee, Stephen Beungtae Ryu, Inhwan Hwang, Daniel P Schachtman
Protein transport between organelles is an essential process in all eukaryotic cells and is mediated by the regulation of processes such as vesicle formation, transport, docking and fusion. In animals, SCY1-Like2 (SCYL2) binds to clathrin and has been shown to play roles in trans-Golgi network (TGN)-mediated clathrin coated vesicle (CCV) trafficking. Here we demonstrate that SCYL2A and SCYL2B, which are Arabidopsis homologs of animal SCYL2, are vital for plant cell growth and root hair development. Studies of the SCYL2 isoforms using multiple single or double loss of function alleles show that SCYL2B is involved in root hair development and that SCYL2A and SCYL2B are essential for plant growth and development and act redundantly in those processes...
July 27, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28741477/intergenically-spliced-chimeric-rnas-in-cancer
#9
REVIEW
Yuemeng Jia, Zhongqiu Xie, Hui Li
Gene fusions and their encoded products (fusion RNAs and proteins) are viewed as one of the hallmarks of cancer. Traditionally, they were thought to be generated solely by chromosomal rearrangements. However, recent discoveries of trans-splicing and cis-splicing events between neighboring genes suggest that there are other mechanisms to generate chimeric fusion RNAs without corresponding changes in DNA. In addition, chimeric RNAs have been detected in normal physiology, complicating the use of fusions in cancer detection and therapy...
September 2016: Trends in Cancer
https://www.readbyqxmd.com/read/28738417/a-pharmacodynamic-analysis-of-choroidal-neovascularization-in-a-porcine-model-using-three-targeted-drugs
#10
COMPARATIVE STUDY
Jeffrey Tran, Caroline Craven, Kathy Wabner, Jenn Schmit, Brock Matter, Uday Kompella, Hans E Grossniklaus, Timothy W Olsen
Purpose: To compare the efficacy of microneedle-delivered suprachoroidal (SC) pazopanib to intravitreal (Ivit) delivery of pazopanib, bevacizumab, or a fusion protein hI-con1 versus vehicle controls on choroidal neovascularization (CNV) growth in a pig model. Methods: Forty-one pigs were injected on the day of CNV induction (hI-con1 on postinduction day 14) with either 2.5 mg Ivit bevacizumab (n = 9), 1 mg Ivit pazopanib (n = 9), 300 Ivit μg hI-con1 (n = 4), or 1 mg SC pazopanib (n = 9), vs...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28727848/the-nuclear-proteome-of-trypanosoma-brucei
#11
Carina Goos, Mario Dejung, Christian J Janzen, Falk Butter, Susanne Kramer
Trypanosoma brucei is a protozoan flagellate that is transmitted by tsetse flies into the mammalian bloodstream. The parasite has a huge impact on human health both directly by causing African sleeping sickness and indirectly, by infecting domestic cattle. The biology of trypanosomes involves some highly unusual, nuclear-localised processes. These include polycistronic transcription without classical promoters initiated from regions defined by histone variants, trans-splicing of all transcripts to the exon of a spliced leader RNA, transcription of some very abundant proteins by RNA polymerase I and antigenic variation, a switch in expression of the cell surface protein variants that allows the parasite to resist the immune system of its mammalian host...
2017: PloS One
https://www.readbyqxmd.com/read/28720994/early-migration-of-bone-graft-causing-sigmoid-colon-perforation-after-trans-foraminal-lumbar-interbody-fusion
#12
Bhavuk Garg, Amit Singla, Sahil Batra, Senthil Kumar
BACKGROUND: Tran foraminal lumbar interbody fusion (TLIF) is a well accepted and standard technique of achieving spinal fusion using pedicle screws, cage and bone graft. We are presenting here a case of L4-L5 lumbar canal stenosis managed with TLIF that presented with sigmoid colon perforation due to bone graft migration 4 days after surgery. CASE REPORT: A 35 years old female underwent open TLIF (from right side) with decompression for L4-L5 lumbar canal stenosis...
April 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28718762/sec17-sec18-act-twice-enhancing-membrane-fusion-and-then-disassembling-cis-snare-complexes
#13
Hongki Song, Amy Orr, Mengtong Duan, Alexey J Merz, William Wickner
At physiological protein levels, the slow HOPS- and SNARE-dependent fusion which occurs upon complete SNARE zippering is stimulated by Sec17 and Sec18:ATP without requiring ATP hydrolysis. To stimulate, Sec17 needs its central residues which bind the 0-layer of the SNARE complex and its N-terminal apolar loop. Adding a transmembrane anchor to the N-terminus of Sec17 bypasses this requirement for apolarity of the Sec17 loop, suggesting that the loop functions for membrane binding rather than to trigger bilayer rearrangement...
July 18, 2017: ELife
https://www.readbyqxmd.com/read/28711733/expression-and-purification-of-tat-ndrg2-recombinant-protein-and-evaluation-of-its-anti-proliferative-effect-on-lncap-cell-line
#14
Fahimeh Farokhinejad, Abbas Behzad Behbahani, Gholam Reza Rafiei Dehbidi, Mohammad Ali Takhshid
N-myc downstream regulated gene2 (NDRG2) belongs to tumor suppressor protein family of NDRG. Anti-proliferative and anti-metastasis of NDRG2 overexpression has been demonstrated in a number of tumors. The aim of this study was to fuse the gene of Trans Activator of Transcription (TAT) protein transduction domain with NDRG2 gene and express and purify TAT-NDRG2 fusion protein in order to investigate the effects of TAT-NDRG2 protein on proliferation and apoptosis of LNCaP prostate carcinoma cell line. pET28a-TAT-NDRG2 and pET28a-NDRG2 plasmids were constructed and transformed into E...
October 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28698658/intercellular-adhesion-molecule-1-augments-myoblast-adhesion-and-fusion-through-homophilic-trans-interactions
#15
Francis X Pizza, Ryan A Martin, Evan M Springer, Maxwell S Leffler, Bryce R Woelmer, Isaac J Recker, Douglas W Leaman
The overall objective of the study was to identify mechanisms through which intercellular adhesion molecule-1 (ICAM-1) augments the adhesive and fusogenic properties of myogenic cells. Hypotheses were tested using cultured myoblasts and fibroblasts, which do not constitutively express ICAM-1, and myoblasts and fibroblasts forced to express full length ICAM-1 or a truncated form lacking the cytoplasmic domain of ICAM-1. ICAM-1 mediated myoblast adhesion and fusion were quantified using novel assays and cell mixing experiments...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28696354/a-case-of-aml-characterized-by-a-novel-t-4-15-q31-q22-translocation-that-confers-a-growth-stimulatory-response-to-retinoid-based-therapy
#16
Justin M Watts, Aymee Perez, Lutecia Pereira, Yao-Shan Fan, Geoffrey Brown, Francisco Vega, Kevin Petrie, Ronan T Swords, Arthur Zelent
Here we report the case of a 30-year-old woman with relapsed acute myeloid leukemia (AML) who was treated with all-trans retinoic acid (ATRA) as part of investigational therapy (NCT02273102). The patient died from rapid disease progression following eight days of continuous treatment with ATRA. Karyotype analysis and RNA-Seq revealed the presence of a novel t(4;15)(q31;q22) reciprocal translocation involving the TMEM154 and RASGRF1 genes. Analysis of primary cells from the patient revealed the expression of TMEM154-RASGRF1 mRNA and the resulting fusion protein, but no expression of the reciprocal RASGRF1-TMEM154 fusion...
July 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28696118/fusion-of-ultraviolet-visible-and-infrared-transient-absorption-spectroscopy-data-to-model-ultrafast-photoisomerization
#17
Bruno Debus, Maylis Orio, Julien Rehault, Gotard Burdzinski, Cyril Ruckebusch, Michel Sliwa
Ultrafast photoisomerization reactions generally start at a higher excited state with excess of internal vibrational energy and occur via conical intersections. This leads to ultrafast dynamics which are difficult to investigate with a single transient absorption spectroscopy technique, be it in the ultraviolet-visible (UV-vis) or infrared (IR) domain. On one hand, the information available in the UV-vis domain is limited as only slight spectral changes are observed for different isomers. On the other hand, the interpretation of vibrational spectra is strongly hindered by intramolecular relaxation and vibrational cooling...
July 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28688485/continuous-detection-of-human-fall-using-multimodal-features-from-kinect-sensors-in-scalable-environment
#18
Thanh-Hai Tran, Thi-Lan Le, Van-Nam Hoang, Hai Vu
BACKGROUND AND OBJECTIVES: Automatic detection of human fall is a key problem in video surveillance and home monitoring. Existing methods using unimodal data (RGB / depth / skeleton) may suffer from the drawbacks of inadequate lighting condition or unreliability. Besides, most of proposed methods are constrained to a small space with off-line video stream. METHODS: In this study, we overcome these encountered issues by combining multi-modal features (skeleton and RGB) from Kinect sensor to take benefits of each data characteristic...
July 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28687976/annexin-a2-s100a10-heterotetramer-is-upregulated-by-pml-rar%C3%AE-fusion-protein-and-promotes-plasminogen-dependent-fibrinolysis-and-matrix-invasion-in-acute-promyelocytic-leukemia
#19
Dan Huang, Yan Yang, Jian Sun, Xiaorong Dong, Jiao Wang, Hongchen Liu, Chengquan Lu, Xueyu Chen, Jing Shao, Jinsong Yan
Aberrant expression of annexin A2-S100A10 heterotetramer (AIIt) associated with PML/RARα fusion protein causes lethal hyperfibrinolysis in acute promyelocytic leukemia (APL), but the mechanism is unclear. To facilitate the investigation of regulatory association between ANXA2 and promyelocytic leukemia/retinoic acid receptor α (PML/RARα) fusion protein, this work was performed to determine the transcription start site of ANXA2 promoter with rapid amplification of 5'-cDNA ends analysis. Zinc-induced U937/PR9 cells expressed PML/RARα fusion protein, and resultant increases in ANXA2 transcripts and translational expressions of both ANXA2 and S100A10, while S100A10 transcripts remained constitutive...
July 8, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28637767/a-short-region-upstream-of-the-yeast-vacuolar-qa-snare-heptad-repeats-promotes-membrane-fusion-through-enhanced-snare-complex-assembly
#20
Hongki Song, William Wickner
Whereas SNARE (soluble N -ethylmaleimide-sensitive factor attachment protein receptor) heptad-repeats are well studied, SNAREs also have upstream N-domains of indeterminate function. The assembly of yeast vacuolar SNAREs into complexes for fusion can be studied in chemically defined reactions. Complementary proteoliposomes bearing a Rab:GTP and either the vacuolar R-SNARE or one of the three integrally anchored Q-SNAREs were incubated with the tethering/SM protein complex HOPS and the two other soluble SNAREs (lacking a transmembrane anchor) or their SNARE heptad-repeat domains...
August 15, 2017: Molecular Biology of the Cell
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