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https://www.readbyqxmd.com/read/27663837/development-and-validation-of-a-novel-single-nucleotide-polymorphism-snp-panel-for-genetic-analysis-of-blastomyces-spp-and-association-analysis
#1
Holly M Frost, Jennifer L Anderson, Lynn Ivacic, Brian L Sloss, John Embil, Jennifer K Meece
BACKGROUND: Single nucleotide polymorphism (SNP) genotyping is increasingly being utilized for molecular typing of pathogens and is cost-effective, especially for large numbers of isolates. The goals of this study were 1) to develop and validate a SNP assay panel for genetic analysis of Blastomyces spp., 2) ascertain whether microsatellite genotyping and the SNP genotyping with the developed panel resolve identical genetic groups, and 3) explore the utility of SNPs for examining phylogenetic and virulence questions in humans...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27213039/assessing-the-magnitude-and-trends-in-hospital-acquired-infections-in-canadian-hospitals-through-sequential-point-prevalence-surveys
#2
Geoffrey Taylor, Denise Gravel, Anne Matlow, Joanne Embree, Nicole LeSaux, Lynn Johnston, Kathryn N Suh, Michael John, John Embil, Elizabeth Henderson, Virginia Roth, Alice Wong
BACKGROUND: Healthcare acquired infections (HAI) are an important public health problem in developed countries, but comprehensive data on trends over time are lacking. Prevalence surveys have been used as a surrogate for incidence studies and can be readily repeated. METHODS: The Canadian Nosocomial Infection Surveillance Program conducted prevalence surveys in 2002 and 2009 in a large network of major Canadian acute care hospitals. NHSN definitions of HAI were used...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27149459/management-and-outcomes-of-acute-respiratory-distress-syndrome-caused-by-blastomycosis-a-retrospective-case-series
#3
Ilan S Schwartz, John M Embil, Atul Sharma, Stephen Goulet, R Bruce Light
Acute respiratory distress syndrome (ARDS) is an uncommon, highly fatal, and poorly understood manifestation of blastomycosis. Optimal management remains unknown, including the roles of adjunctive corticosteroids and extracorporeal membrane oxygenation (ECMO).We conducted a retrospective chart review of patients with ARDS caused by blastomycosis, managed in intensive care units in Manitoba, Canada, from 1992 to 2014. ARDS was defined using the Berlin definition. Corticosteroid therapy was defined as ≥150 mg cortisol equivalent in 24 hours...
May 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/26386266/iwgdf-guidance-on-the-diagnosis-and-management-of-foot-infections-in-persons-with-diabetes
#4
Benjamin A Lipsky, Javier Aragón-Sánchez, Mathew Diggle, John Embil, Shigeo Kono, Lawrence Lavery, Éric Senneville, Vilma Urbančič-Rovan, Suzanne Van Asten, Edgar J G Peters
No abstract text is available yet for this article.
January 2016: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/26344844/interventions-in-the-management-of-infection-in-the-foot-in-diabetes-a-systematic-review
#5
REVIEW
E J Peters, B A Lipsky, J Aragón-Sánchez, E J Boyko, M Diggle, J M Embil, S Kono, L A Lavery, E Senneville, V Urbančič-Rovan, S A Van Asten, W J Jeffcoate
The expert panel on diabetic foot infection (DFI) of the International Working Group on the Diabetic Foot conducted a systematic review seeking all published reports relating to any type of treatment for infection of the foot in persons with diabetes published as of 30 June 2014. This review, conducted with both PubMed and EMBASE, was used to update an earlier one undertaken on 30 June 2010 using the same search string. Eligible publications included those that had outcome measures reported for both a treated and a control population that were managed either at the same time, or as part of a before-and-after case design...
January 2016: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/26015796/empyema-caused-by-clostridium-bifermentans-a-case-report
#6
Safa Edagiz, Phil Lagace-Wiens, John Embil, James Karlowsky, Andrew Walkty
A case of pneumonia with associated empyema caused by Clostridium bifermentans is described. C bifermentans is an anaerobic, spore-forming, Gram-positive bacillus. This organism is infrequently reported as a cause of infection in humans, and older publications tended to regard it as nonpathogenic. However, in more recent reports, C bifermentans has been documented as a cause of septic arthritis, osteomyelitis, soft tissue infection, abdominal infections, brain abscess, bacteremia and endocarditis. The present case is the third reported case of empyema caused by C bifermentans, and it serves to further define the spectrum of illness due to this uncommon organism...
March 2015: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/26015790/prevalence-of-antimicrobial-use-in-a-network-of-canadian-hospitals-in-2002-and-2009
#7
Geoffrey Taylor, Denise Gravel, Lynora Saxinger, Kathryn Bush, Kimberley Simmonds, Anne Matlow, Joanne Embree, Nicole Le Saux, Lynn Johnston, Kathryn N Suh, John Embil, Elizabeth Henderson, Michael John, Virginia Roth, Alice Wong
BACKGROUND: Increasing antimicrobial resistance has been identified as an important global health threat. Antimicrobial use is a major driver of resistance, especially in the hospital sector. Understanding the extent and type of antimicrobial use in Canadian hospitals will aid in developing national antimicrobial stewardship priorities. METHODS: In 2002 and 2009, as part of one-day prevalence surveys to quantify hospital-acquired infections in Canadian Nosocomial Infection Surveillance Program hospitals, data were collected on the use of systemic antimicrobial agents in all patients in participating hospitals...
March 2015: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/25826161/food-consumption-obesity-and-abnormal-glycaemic-control-in-a-canadian-inuit-community
#8
J Singer, C Putulik Kidlapik, B Martin, H J Dean, E Trepman, J M Embil
Dietary and lifestyle factors may contribute to diabetes and obesity in the Canadian Inuit. We documented dietary patterns, physical activity level, obesity, blood glucose abnormalities and diabetes prevalence in a Canadian Inuit community. There were 250 Inuit residents of Repulse Bay, Nunavut, who had an interview about diet and physical activity, measurement of weight and height, and laboratory studies (194 subjects). Children, adolescents and younger adults (aged < 48 years) consumed significantly less country food and more processed snack foods and sweet drinks than older adults (aged ≥ 48 years)...
December 2014: Clinical Obesity
https://www.readbyqxmd.com/read/25798157/pasteurella-species-peritoneal-dialysis-associated-peritonitis-household-pets-as-a-risk-factor
#9
REVIEW
Philippe Guillaume Poliquin, Philippe Lagacé-Wiens, Mauro Verrelli, David W Allen, John M Embil
BACKGROUND: Pasteurella species are Gram-negative coccobacilli that are a part of the normal oropharyngeal flora of numerous domestic animals. They have been recognized as a rare but significant cause of peritonitis in patients undergoing peritoneal dialysis (PD). A consensus about management strategies for PD-associated peritonitis caused by Pasteurella species currently does not exist. METHODS: The microbiological database serving the Manitoba Renal Program was searched from 1997 to 2013 for cases of Pasteurella species PD-associated peritonitis, and charts were reviewed...
January 2015: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/25682407/mental-health-issues-associated-with-foot-complications-of-diabetes-mellitus
#10
Christopher Hoban, Jitender Sareen, Christine A Henriksen, Lorraine Kuzyk, John M Embil, Elly Trepman
BACKGROUND: The purpose of this study was to evaluate the effect of foot problems on mental health in diabetic patients and their caregivers. METHODS: Diabetic patients (47 patients with and 49 patients without foot problems), and 21 caregivers of patients with foot problems, completed outcome surveys. Foot problems included ulcers (41 patients [87%]), osteomyelitis (9 patients [19%]), and Charcot foot (8 patients [17%]). RESULTS: In contrast with diabetic patients having no foot problems, diabetic patients with foot problems had, on average, significantly greater symptoms of diabetes (Diabetes Symptom Checklist-2 score), greater depression symptoms (Hospital Anxiety and Depression Scale [HADS]-Depression score), worse health-related quality of life (Medical Outcome Study Short Form 36 [SF-36]: Physical Component Summary score and 6 of 8 subscales), greater pain (Short-Form McGill Pain Questionnaire), and greater suicidal behavior (Suicidal Behaviors Questionnaire-Revised)...
March 2015: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/25676712/isolation-of-multiple-carbapenemase-producing-gram-negative-bacilli-from-a-patient-recently-hospitalized-in-nigeria
#11
Andrew Walkty, Matthew Gilmour, Patricia Simner, John M Embil, David Boyd, Michael Mulvey, James Karlowsky
A 29-year-old female transferred to a tertiary care hospital in Winnipeg, Canada, after a prolonged period of hospitalization in Nigeria was found to be colonized with a VIM-2-producing Pseudomonas aeruginosa, a NDM-1-producing Klebsiella pneumoniae, and an OXA-181-producing Escherichia coli. Detection of carbapenemase-producing organisms from a rectal swab was accomplished by screening with chromogenic media, followed by confirmation with the Rapid CARB Screen kit (Rosco Diagnostica, Taastrup, Denmark). This case illustrates the need for clinical microbiology laboratories to have a protocol in place to screen patients for carbapenemase producers, even in countries where these organisms are rarely encountered...
April 2015: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/25459678/vitamin-d-binding-protein-polymorphism-protects-against-development-of-blastomycosis
#12
J P Sainsbury, A Trajtman, A T Stalker, J M Embil, Y Keynan
Blastomycosis is an uncommon endemic fungal infection. It is presumed that in the endemic regions, the number of exposed individuals is significantly greater than those in whom clinical manifestations develop. We conducted a case-control study of individuals with clinical blastomycosis and controls with similar exposure but who did not develop disease. A genetic association was observed between the Gc-2 allele of vitamin D binding protein and reduced susceptibility to blastomycosis in a Canadian cohort. The Gc-2 allele can affect increased antimicrobial activity of macrophages...
December 2014: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/25026626/pseudo-outbreak-of-phaeoacremonium-parasiticum-from-a-hospital-ice-dispenser
#13
Molly Blake, John M Embil, Elly Trepman, Heather Adam, Renelle Myers, Pam Mutcher
In 31 patients, Phaeoacremonium parasiticum was recovered from bronchoscopy specimens (biopsies and aspirates). The pseudo-outbreak was caused by contaminated ice used to control hemorrhage during bronchoscopy and was associated with deficiencies in equipment cleaning. The bronchoscopy technique was modified, the ice dispenser was disinfected, bronchoscope reprocessing was improved, and there were no recurrences.
August 2014: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/24634686/an-unusual-case-of-streptococcus-anginosus-group-pyomyositis-diagnosed-using-direct-16s-ribosomal-dna-sequencing
#14
Andrew Walkty, John M Embil, Kim Nichol, James Karlowsky
Bacteria belonging to the Streptococcus anginosus group (Streptococcus intermedius, Streptococcus constellatus and Streptococcus anginosus) are capable of causing serious pyogenic infections, with a tendency for abscess formation. The present article reports a case of S anginosus group pyomyositis in a 47-year-old man. The pathogen was recovered from one of two blood cultures obtained from the patient, but speciation was initially not performed because the organism was considered to be a contaminant (viridans streptococci group)...
2014: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/24591284/hemorrhagic-pustules-in-an-aboriginal-man
#15
REVIEW
Kerry MacDonald, Philippe Lagacé-Wiens, John Embil
No abstract text is available yet for this article.
May 13, 2014: CMAJ: Canadian Medical Association Journal, Journal de L'Association Medicale Canadienne
https://www.readbyqxmd.com/read/24439136/phase-3-study-comparing-tigecycline-and-ertapenem-in-patients-with-diabetic-foot-infections-with-and-without-osteomyelitis
#16
RANDOMIZED CONTROLLED TRIAL
Laszlo Lauf, Zsófia Ozsvár, Ismael Mitha, Janos Regöly-Mérei, John M Embil, Angel Cooper, Mary Beth Sabol, Nathalie Castaing, Nathalie Dartois, Jean Yan, Gary Dukart, Robert Maroko
A phase 3, randomized, double-blind trial was conducted in subjects with diabetic foot infections without osteomyelitis (primary study) or with osteomyelitis (substudy) to determine the efficacy and safety of parenteral (intravenous [iv]) tigecycline (150 mg once-daily) versus 1 g once-daily iv ertapenem ± vancomycin. Among 944 subjects in the primary study who received ≥1 dose of study drug, >85% had type 2 diabetes; ~90% had Perfusion, Extent, Depth/tissue loss, Infection, and Sensation infection grade 2 or 3; and ~20% reported prior antibiotic failure...
April 2014: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/23997772/reducing-the-adverse-impact-of-injection-drug-use-in-canada
#17
Kevin B Laupland, John M Embil
No abstract text is available yet for this article.
2012: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/23566338/outcomes-of-persons-with-blastomycosis-involving-the-central-nervous-system
#18
Jonathan W Bush, Terry Wuerz, John M Embil, Marc R Del Bigio, Patrick J McDonald, Sherry Krawitz
Blastomyces dermatitidis is a dimorphic fungus which is potentially life-threatening if central nervous system (CNS) dissemination occurs. Sixteen patients with proven or probable CNS blastomycosis are presented. Median duration of symptoms was 90 days; headache and focal neurologic deficit were the most common presenting symptoms. Magnetic resonance imaging (MRI) consistently demonstrated an abnormality, compared to 58% of computed tomography scans. Tissue culture yielded the pathogen in 71% of histology-confirmed cases...
June 2013: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/23524466/molecular-epidemiology-of-vancomycin-resistant-enterococcal-bacteraemia-results-from-the-canadian-nosocomial-infection-surveillance-program-1999-2009
#19
M McCracken, A Wong, R Mitchell, D Gravel, J Conly, J Embil, L Johnston, A Matlow, D Ormiston, A E Simor, S Smith, T Du, R Hizon, M R Mulvey
OBJECTIVES: Vancomycin-resistant enterococci (VRE) can be associated with serious bacteraemia. The focus of this study was to characterize the molecular epidemiology of VRE from bacteraemia cases that were isolated from 1999 to 2009 as part of Canadian Nosocomial Infection Surveillance Program (CNISP) surveillance activities. METHODS: From 1999 to 2009, enterococci were collected from across Canada in accordance with the CNISP VRE surveillance protocol. MICs were determined using broth microdilution...
July 2013: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/23365589/guidelines-for-the-prevention-and-management-of-community-associated-methicillin-resistant-staphylococcus-aureus-a-perspective-for-canadian-health-care-practitioners
#20
Michelle Barton, Michael Hawkes, Dorothy Moore, John Conly, Lindsay Nicolle, Upton Allen, Nora Boyd, Joanne Embree, Liz Van Horne, Nicole Le Saux, Susan Richardson, Aideen Moore, Dat Tran, Valerie Waters, Mary Vearncombe, Kevin Katz, J Scott Weese, John Embil, Marianna Ofner-Agostini, E Lee Ford-Jones
No abstract text is available yet for this article.
September 2006: Canadian Journal of Infectious Diseases & Medical Microbiology
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