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https://www.readbyqxmd.com/read/28330141/evaluation-of-biochemical-markers-during-somatic-embryogenesis-in-silybum-marianum-l
#1
Bilal Haider Abbasi, Huma Ali, Buhara Yücesan, Sabahat Saeed, Khalid Rehman, Mubarak Ali Khan
In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic embryogenesis. Highest callus induction frequency (76 ± 4.8 %) was recorded when petiole explants of in vitro derived plantlets were cultured on B5 medium supplemented with 1...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28303758/beneficial-effect-of-n-3-polyunsaturated-fatty-acids-on-inflammation-and-analgesic-use-in-psoriatic-arthritis-a-randomized-double-blind-placebo-controlled-trial
#2
S Kristensen, E B Schmidt, A Schlemmer, C Rasmussen, M B Johansen, J H Christensen
OBJECTIVE: This study aimed to investigate the effects of marine n-3 polyunsaturated fatty acids (PUFAs) on disease activity, use of analgesics, and inflammatory biomarkers in patients with psoriatic arthritis (PsA). METHOD: Patients with established PsA (n = 145) were investigated in a randomized, double-blind, placebo-controlled study. The participants received a supplement of 3 g n-3 PUFA/day or 3 g olive oil/day (control) for 24 weeks. Outcome measures for disease activity, use of analgesics, and leukotriene formation from activated granulocytes were assessed at baseline and at study end...
March 17, 2017: Scandinavian Journal of Rheumatology
https://www.readbyqxmd.com/read/28291742/first-principles-study-of-methane-decomposition-on-b5-step-edge-type-site-of-ru-surface
#3
Ryan Arevalo, Susan Aspera, Mary Clare Escaño, Hiroshi Nakanishi, Hideaki Kasai
Many chemical reactions that produce a wide range of hydrocarbons and alcohols involve the breaking of C-H bonds in methane. In this paper, we analyzed the decomposition of this molecule on the B5 step-edge type site of Ru surface using first principles calculations based on dispersion-corrected density functional theory. Methane was found to be weakly adsorbed on the surface, characterized by the hybridization of its sp states with Ru-d<sub>xz,yz,zz</sub> states. Dissociative adsorption is energetically preferred over molecular methane adsorption, resulting in CH fragment...
March 14, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28275786/-effects-of-repeated-firing-on-microleakage-of-selective-laser-melting-ceramic-crowns
#4
Qun Zhong, Yan Peng, Xue-Ying Wu, Jia-Wei Weng
PURPOSE: To investigate the effects of repeated firing on microleakage of selective laser melting ceramic crowns. METHODS: Fifty molars were randomly divided into 2 groups (25 teeth in each group). Teeth in group A received a chamfer finish line preparation, whereas teeth in group B received a shoulder finish line. After SLM metal crowns were fabricated, all the crowns received initial oxidation step, opaque firing, dentin firing and glaze firing, then crowns in each group were randomly divided into 5 sub-groups according to different time of clinical firings...
December 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/28275666/data-representing-two-separate-lc-ms-methods-for-detection-and-quantification-of-water-soluble-and-fat-soluble-vitamins-in-tears-and-blood-serum
#5
Maryam Khaksari, Lynn R Mazzoleni, Chunhai Ruan, Robert T Kennedy, Adrienne R Minerick
Two separate liquid chromatography (LC)-mass spectrometry (MS) methods were developed for determination and quantification of water-soluble and fat-soluble vitamins in human tear and blood serum samples. The water-soluble vitamin method was originally developed to detect vitamins B1, B2, B3 (nicotinamide), B5, B6 (pyridoxine), B7, B9 and B12 while the fat-soluble vitamin method detected vitamins A, D3, 25(OH)D3, E and K1. These methods were then validated with tear and blood serum samples. In this data in brief article, we provide details on the two LC-MS methods development, methods sensitivity, as well as precision and accuracy for determination of vitamins in human tears and blood serum...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28260494/hemoglobin-and-myoglobin-as-reducing-agents-in-biological-systems-redox-reactions-of-globins-with-copper-and-iron-salts-and-complexes
#6
REVIEW
G B Postnikova, E A Shekhovtsova
In addition to reversible O2 binding, respiratory proteins of the globin family, hemoglobin (Hb) and myoglobin (Mb), participate in redox reactions with various metal complexes, including biologically significant ones, such as those of copper and iron. HbO2 and MbO2 are present in cells in large amounts and, as redox agents, can contribute to maintaining cell redox state and resisting oxidative stress. Divalent copper complexes with high redox potentials (E0, 200-600 mV) and high stability constants, such as [Cu(phen)2](2+), [Cu(dmphen)2](2+), and CuDTA oxidize ferrous heme proteins by the simple outer-sphere electron transfer mechanism through overlapping π-orbitals of the heme and the copper complex...
December 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28259077/assessment-of-the-efficacy-of-membrane-filtration-processes-to-remove-human-enteric-viruses-and-the-suitability-of-bacteriophages-and-a-plant-virus-as-surrogates-for-those-viruses
#7
N Shirasaki, T Matsushita, Y Matsui, K Murai
Here, we evaluated the efficacy of direct microfiltration (MF) and ultrafiltration (UF) to remove three representative human enteric viruses (i.e., adenovirus [AdV] type 40, coxsackievirus [CV] B5, and hepatitis A virus [HAV] IB), and one surrogate of human caliciviruses (i.e., murine norovirus [MNV] type 1). Eight different MF membranes and three different UF membranes were used. We also examined the ability of coagulation pretreatment with high-basicity polyaluminum chloride (PACl) to enhance virus removal by MF...
February 24, 2017: Water Research
https://www.readbyqxmd.com/read/28241869/de-novo-transcriptome-assembly-functional-annotation-and-differential-gene-expression-analysis-of-juvenile-and-adult-e-fetida-a-model-oligochaete-used-in-ecotoxicological-studies
#8
Michelle Thunders, Jo Cavanagh, Yinsheng Li
BACKGROUND: Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. RESULTS: This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool...
February 27, 2017: Biological Research
https://www.readbyqxmd.com/read/28238727/testosterone-metabolism-of-equine-single-cyps-of-the-3a-subfamily-compared-to-the-human-cyp3a4
#9
S Vimercati, M Büchi, J Zielinski, N Peduto, M Mevissen
Cytochrome P450 enzymes (CYPs) are responsible for the phase I metabolism of drugs, xenobiotics and endogenous substances. Knowledge of single CYPs and their substrates is important for drug metabolism, helps to predict adverse effects and may prevent reduced drug efficacy in polypharmacy. In this study, three equine isoenzymes of the 3A subfamily, the equine flavoprotein NADPH-P450 oxidoreductase (POR), and the cytochrome b5 (CYB5) were cloned, sequenced and heterologously expressed in a baculovirus expression system...
February 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28238672/thermodynamics-of-interactions-between-mammalian-cytochromes-p450-and-b5
#10
Evgeny Yablokov, Anna Florinskaya, Alexei Medvedev, Gennady Sergeev, Natallia Strushkevich, Alexander Luschik, Tatsiana Shkel, Irina Haidukevich, Andrei Gilep, Sergey Usanov, Alexis Ivanov
Cytochromes P450 (CYPs) play an important role in the metabolism of xenobiotics and various endogenous substrates. Being a crucial component of the microsomal monooxygenase system, CYPs are involved in numerous protein-protein interactions. However, mechanisms underlying molecular interactions between components of the monooxygenase system still need better characterization. In this study thermodynamic parameters of paired interactions between mammalian CYPs and cytochromes b5 (CYB5) have been evaluated using a Surface Plasmon Resonance (SPR) based biosensor Biacore 3000...
February 24, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28237118/using-photoconvertible-and-extractable-fluorescent-proteins-to-study-autophagy-in-plants
#11
M O Abiodun, K Matsuoka
Several methodologies have been employed to understand the kinetics of induced autophagic degradation in plants, but most of them are not capable of distinguishing the autophagic cargo proteins before and after induction of autophagy in cells. Here, we designed a mass photoconverter that allowed us to simultaneously monitor protein synthesis and degradation in tobacco BY-2 cells using a photoconvertible fluorescence marker protein, Kikume Green Red (KikGR). An example of a new protocol for the analysis of autophagy progression using a fusion protein of cytochrome b5 and KikGR under phosphate starvation is described...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28225078/distribution-of-valence-electrons-of-the-flavin-cofactor-in-nadh-cytochrome-b5-reductase
#12
Kiyofumi Takaba, Kazuki Takeda, Masayuki Kosugi, Taro Tamada, Kunio Miki
Flavin compounds such as flavin adenine dinucleotide (FAD), flavin mononucleotide and riboflavin make up the active centers in flavoproteins that facilitate various oxidoreductive processes. The fine structural features of the hydrogens and valence electrons of the flavin molecules in the protein environment are critical to the functions of the flavoproteins. However, information on these features cannot be obtained from conventional protein X-ray analyses at ordinary resolution. Here we report the charge density analysis of a flavoenzyme, NADH-cytochrome b5 reductase (b5R), at an ultra-high resolution of 0...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216158/resveratrol-regulates-neuronal-glucose-uptake-and-insulin-sensitivity-via-p21-activated-kinase-2-pak2
#13
Pallavi Varshney, Chinmoy Sankar Dey
We have recently reported P21-activated kinase 2 (PAK2), a serine/threonine kinase as a negative regulator of neuronal glucose uptake and insulin sensitivity. Resveratrol (RSV), a natural polyphenol with anti-oxidative, anti-inflammatory and anti-diabetic properties, regulates PAK2 activity in HepG2 and ESC-B5 cell apoptosis. However, regulation of PAK2 by RSV in neuronal insulin signaling pathway, if any, is still unknown. In the present study, RSV treatment significantly increased PAK2 activity under insulin-sensitive and insulin-resistant condition, along with a marked decrease in glucose uptake in differentiated N2A cells...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28161630/base-pair-opening-dynamics-of-the-microrna-precursor-pri-mir156a-affect-temperature-responsive-flowering-in-arabidopsis
#14
Hee-Eun Kim, Wanhui Kim, Ae-Ree Lee, Suhyun Jin, A Rim Jun, Nak-Kyoon Kim, Joon-Hwa Lee, Ji Hoon Ahn
Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains largely unknown. Here, we performed nuclear magnetic resonance studies to investigate the base-pair opening dynamics of model RNAs at 16 °C and investigated the in vivo effects of the mutant RNAs on temperature-responsive flowering...
February 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28134754/fabrication-of-annealed-gold-nanostructures-on-pre-treated-glow-discharge-cleaned-glasses-and-their-used-for-localized-surface-plasmon-resonance-lspr-and-surface-enhanced-raman-spectroscopy-sers-detection-of-adsorbed-bio-molecules
#15
Rodica Elena Ionescu, Ece Neslihan Aybeke, Eric Bourillot, Yvon Lacroute, Eric Lesniewska, Pierre-Michel Adam, Jean-Louis Bijeon
Metallic nanoparticles are considered as active supports in the development of specific chemical or biological biosensors. Well-organized nanoparticles can be prepared either through expensive (e.g., electron beam lithography) or inexpensive (e.g., thermal synthesis) approaches where different shapes of nanoparticles are easily obtained over large solid surfaces. Herein, the authors propose a low-cost thermal synthesis of active plasmonic nanostructures on thin gold layers modified glass supports after 1 h holding on a hot plate (~350 °C)...
January 26, 2017: Sensors
https://www.readbyqxmd.com/read/28112125/systemic-administration-of-an-anti-tumor-necrosis-factor-alpha-monoclonal-antibody-protects-against-endotoxin-induced-uveitis-in-rats
#16
Qingman Ge, Shaocheng Wang, Yuezhong Zheng
OBJECTIVE: This study was to evaluate the effect of systemic injection of an anti-tumor necrosis factor alpha (TNF-α) monoclonal antibody (mAb) on endotoxin-induced uveitis (EIU). MATERIALS AND METHODS: Fifty-six male Wistar rats (6-8 weeks old) were randomly divided into three groups: EIU, anti-TNF-α mAb + EIU, and control. EIU was induced by injecting Escherichia coli O55:B5 lipopolysaccharide (LPS) into the hind footpad of the rats (150 μg/rat). The anti-TNF-α mAb (1 μg/kg) was administrated 30 min before LPS injection through one-time intravenous injection...
December 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28107436/a-micro-ct-study-in-patients-with-breast-microcalcifications-using-a-mathematical-algorithm-to-assess-3d-structure
#17
David Kenkel, Zsuzsanna Varga, Heike Heuer, Konstantin J Dedes, Nicole Berger, Lukas Filli, Andreas Boss
PURPOSE: The aim of this study was to evaluate the relevance of the three-dimensional (3D) structure of breast microcalcifications (MC) as a predictor of malignancy using highly resolved micro-computed tomography (micro-CT) datasets of biopsy samples. MATERIAL AND METHODS: The study included 28 women with suspicious MC in their mammogram undergoing vacuum-assisted biopsy. Directly after the intervention, the specimens were scanned in a micro-CT with an isometric spatial resolution of 9 μm...
2017: PloS One
https://www.readbyqxmd.com/read/28041733/glucose-requirements-of-an-activated-immune-system-in-lactating-holstein-cows
#18
S K Kvidera, E A Horst, M Abuajamieh, E J Mayorga, M V Sanz Fernandez, L H Baumgard
Accurately quantifying activated immune system energy requirements in vivo is difficult, but a better understanding may advance strategies to maximize animal productivity. Study objectives were to estimate whole-body glucose utilization following an i.v. endotoxin challenge. Lactating Holstein cows were jugular catheterized and assigned 1 of 3 bolus treatments: control (CON; 5 mL of saline; n = 6), lipopolysaccharide (LPS)-administered (LPS-C; 1.5 μg/kg of body weight; Escherichia coli 055:B5; n = 6), and LPS + euglycemic clamp (LPS-Eu; 1...
March 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28031266/chronic-plasmodium-chabaudi-infection-generates-cd4-memory-t-cells-with-increased-t-cell-receptor-sensitivity-but-poor-secondary-expansion-and-increased-apoptosis
#19
Michael M Opata, Robin Stephens
Exposure to blood-stage malaria infection is often persistent, leading to generation of CD4 effector and effector memory T cells that contribute to protection. We showed previously that chronic exposure to blood-stage Plasmodium chabaudi offers the best protection from parasitemia and pathology in reinfection cases, correlating with an increase in Th1 cells. Although much is known about the features of resting or exhausted memory T cells (Tmem), little is known about the functional capacities of chronically stimulated but protective T cells...
March 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28027470/performance-of-a-novel-atrazine-induced-cerebellar-toxicity-in-quail-coturnix-c-coturnix-activating-pxr-car-pathway-responses-and-disrupting-cytochrome-p450-homeostasis
#20
Jun Xia, Lei Qin, Zheng-Hai Du, Jia Lin, Xue-Nan Li, Jin-Long Li
Atrazine is well known to be a biologically hazardous substance with toxic effects, but atrazine-induced neurotoxicity remains unclear. The aim of this study was to investigate the mechanisms of atrazine-induced cerebellar toxicity. To determine atrazine-exerted potential neurotoxicity, quails were treated with 50, 250 and 500 mg/kg atrazine by gavage administration for 45 days. Notably, the changes of cytochrome P450 enzyme system (CYP450s) were observed in atrazine-exposed quails. The contents of cytochrome P450 (CYP450) and Cytochrome b5 (Cyt b5) and the activities of NADPH-cytochrome c reductase (NCR), aminopyrin N-demethylase (APND) and aniline-4-hydeoxylase (AH) were increased and erythromycin N-demethylase (ERND) was decreased in quail cerebellum...
December 18, 2016: Chemosphere
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