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https://www.readbyqxmd.com/read/28646173/transmembrane-interactions-of-full-length-mammalian-bitopic-cytochrome-p450-cytochrome-b5-complex-in-lipid-bilayers-revealed-by-sensitivity-enhanced-dynamic-nuclear-polarization-solid-state-nmr-spectroscopy
#1
Kazutoshi Yamamoto, Marc A Caporini, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy
The dynamic protein-protein and protein-ligand interactions of integral bitopic membrane proteins with a single membrane-spanning helix play a plethora of vital roles in the cellular processes associated with human health and diseases, including signaling and enzymatic catalysis. While an increasing number of high-resolution structural studies of membrane proteins have successfully manifested an in-depth understanding of their biological functions, intact membrane-bound bitopic protein-protein complexes pose tremendous challenges for structural studies by crystallography or solution NMR spectroscopy...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642677/a-novel-workflow-combining-plaque-imaging-plaque-and-plasma-proteomics-identifies-biomarkers-of-human-coronary-atherosclerotic-plaque-disruption
#2
Regent Lee, Roman Fischer, Philip D Charles, David Adlam, Alessandro Valli, Katalin Di Gleria, Rajesh K Kharbanda, Robin P Choudhury, Charalambos Antoniades, Benedikt M Kessler, Keith M Channon
BACKGROUND: Atherosclerotic plaque rupture is the culprit event which underpins most acute vascular syndromes such as acute myocardial infarction. Novel biomarkers of plaque rupture could improve biological understanding and clinical management of patients presenting with possible acute vascular syndromes but such biomarker(s) remain elusive. Investigation of biomarkers in the context of de novo plaque rupture in humans is confounded by the inability to attribute the plaque rupture as the source of biomarker release, as plaque ruptures are typically associated with prompt down-stream events of myocardial necrosis and systemic inflammation...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28638761/peroxidase-activity-of-a-c-type-cytochrome%C3%A2-b5-in-the-non-native-state-is-comparable-to-that-of-native-peroxidases
#3
Shan Hu, Bo He, Ke-Jie Du, Xiao-Juan Wang, Shu-Qin Gao, Ying-Wu Lin
The design of artificial metalloenzymes has achieved tremendous progress, although few designs can achieve catalytic performances comparable to that of native enzymes. Moreover, the structure and function of artificial metalloenzymes in non-native states has rarely been explored. Herein, we found that a c-type cytochrome b5 (Cyt b5), N57C/S71C Cyt b5, with heme covalently attached to the protein matrix through two Cys-heme linkages, adopts a non-native state with an open heme site after guanidine hydrochloride (Gdn⋅HCl)-induced unfolding, which facilitates H2O2 activation and substrate binding...
June 2017: ChemistryOpen
https://www.readbyqxmd.com/read/28627806/improving-the-quantum-yields-of-perylene-diimide-aggregates-by-increasing-molecular-hydrophobicity-in-polar-media
#4
Jodi Szarko, Jürgen Plenge, Xiaoyu Xu, Ashli Austin, Steven Mylon
Here we report the quantum yield of four aggregated perylene diimide (PDI) species that vary by the length of the branched side chains attached at the N,N' imide positions. The PDI molecules were dissolved in binary water:methanol solvents as a means to vary the solvent polarity and control the degree of aggregation in solution. By performing spectroscopy, kinetics, and light scattering experiments, the nature of the molecular interactions in the solutions was determined. The maximum quantum yield of the aggregated molecules increased from 0...
June 19, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28609197/the-glu331del-mutation-in-the-cyp17a1-gene-causes-atypical-congenital-adrenal-hyperplasia-in-a-46-xx-female
#5
Giulia Lanzolla, Giuseppe Vancieri, Silvia Lanciotti, Federica Sangiuolo, Elisa Menegatti, Luca Federici, Costanzo Moretti, Francesco Brancati
17α-Hydroxylase deficiency is an uncommon type of congenital adrenal hyperplasia (CAH) caused by mutations in the CYP17A1 gene encoding both 17α-hydroxylase and 17,20-lyase, essential for sex steroids production. Main clinical features include lack of pubertal development, hypertension, and hypokalemia. We report the first case of a 46,XX female homozygote for the p.Glu331del mutation in the CYP17A1 gene showing an atypical clinical presentation. She was evaluated the first time for primary amenorrhea and delayed puberty in the presence of low levels of androgens, 17β-estradiol, serum cortisol, and high levels of progesterone and gonadotropins...
June 13, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28605979/phytochemical-investigation-of-rumex-thyrsiflorus-fingerh
#6
Orsolya Orbán-Gyapai, Peter Forgo, Judit Hohmann, Andrea Vasas
In the course of our pharmacological screening of Polygonaceae species occurring in the Carpathian Basin the extracts prepared from the roots of Rumex thyrsiflorus showed promising antiproliferative, xanthine oxidase inhibitory and antibacterial activities. The present work deals with the isolation of compounds from the root of the plant. After multistep separation process, four compounds were obtained from the n-hexane, chloroform and ethyl acetate soluble fractions of the methanol extract of the root. The structures of the isolated compounds were determined as 1-palmitoylglycerol, β-sitosterol, (-)-epicatechin, and procyanidin B5...
June 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28590396/botulinum-toxin-conjugated-with-silk-fibroin-and-4-hexylresorcinol
#7
You-Young Jo, Seong-Gon Kim, Min-Keun Kim
PURPOSE: The objective of this study was to evaluate whether silk fibroin (SF) incorporated into 4-hexylresorcinol (4HR) could increase botulinum toxin-A (BTX-A) activity. MATERIAL AND METHODS: In total, 30 rats were used for this study. The animals were divided into 6 groups according to the injected materials (SA: saline only; SF; 4HR; B2: 2 units of BTX-A; B2 + SF + 4HR: combination of B2, SF, and 4HR; B5: 5 units of BTX-A). Serial sonography was used for the evaluation of muscle thickness after injection...
June 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28584062/cytochrome-b5-reductase-3-modulates-soluble-guanylate-cyclase-redox-state-and-cgmp-signaling
#8
Mizanur M Rahaman, Anh T Nguyen, Megan P Miller, Scott A Hahn, Courtney Sparacino-Watkins, Soma Jobbagy, Nolan T Carew, Nadiezhda Cantu-Medellin, Katherine C Wood, Catherine Baty, Francisco J Schopfer, Eric E Kelley, Mark T Gladwin, Emil Martin, Adam C Straub
Rationale: Soluble guanylate cyclase (sGC) heme iron, in its oxidized state (Fe(3+)), is desensitized to nitric oxide (NO) and limits cyclic guanosine 3', 5'-monophosphate (cGMP) production needed for downstream activation of PKG-dependent signaling and blood vessel dilation. Objective: While reactive oxygen species are known to oxidize the sGC heme iron, the basic mechanism(s) governing sGC heme iron recycling to its NO-sensitive, reduced state, remain poorly understood. Methods and Results: Oxidant challenge studies show vascular smooth muscle cells have an intrinsic ability to reduce oxidized sGC heme iron and form protein-protein complexes between cytochrome b5 reductase 3 (Cyb5R3), also known as methemoglobin reductase, and oxidized sGC...
June 5, 2017: Circulation Research
https://www.readbyqxmd.com/read/28583712/structural-influences-on-the-activity-of-bismuth-iii-indole-carboxylato-complexes-towards-helicobacter-pylori-and-leishmania
#9
Amita Pathak, Victoria L Blair, Richard L Ferrero, Lukasz Kedzierski, Philip C Andrews
Seven new bismuth(III) complexes derived from indole-carboxylic acids have been synthesised: five are homoleptic; [Bi(IAA)3] B1, [Bi(IPA)3] B2, [Bi(IBA)3] B3, [Bi(MICA)3] B4, [Bi(IGA)3] B6, and two are heteroleptic [BiPh(MICA)2] B5 (where IAA-H=2-(1H-indol-3-yl)acetic acid, IPA-H=3-(1H-indol-3-yl)propanoic acid, IBA-H=4-(1H-indol-3-yl)butanoic acid, IGA-H=2-(1H-indol-3-yl)-2-oxoacetic acid, and MICA-H=1-methyl-1H-indole-3-carboxylic acid). All complexes were fully characterised by elemental analysis, infrared and mass-spectroscopy, and nuclear magnetic resonance ((1)H, (13)C) spectroscopy...
May 31, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28574589/enterovirus-genotypes-among-patients-with-severe-acute-respiratory-illness-influenza-like-illness-and-asymptomatic-individuals-in-south-africa-2012-2014
#10
Orienka Hellferscee, Stefano Tempia, Sibongile Walaza, Ebrahim Variava, Halima Dawood, Nicole Wolter, Shabir A Madhi, Mignon du Plessis, Cheryl Cohen, Florette K Treurnicht
Enteroviruses can cause outbreaks of severe acute respiratory illness (SARI) and EV-A, -B, -C and -D species have different pathogenic profiles and circulation patterns. We aimed to characterize and determine the prevalence of enterovirus genotypes among South African patients with respiratory illness and controls during June 2012 to July 2014. Syndromic SARI and influenza-like illness (ILI) surveillance was performed at two sentinel sites. At each site nasopharyngeal/oropharyngeal specimens were collected from SARI and ILI patients as well as controls...
June 2, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28543941/evidence-of-structure-sensitivity-in-the-fischer-tropsch-reaction-on-model-cobalt-nanoparticles-by-time-resolved-chemical-transient-kinetics
#11
Walter T Ralston, Gérôme Melaet, Tommy Saephan, Gabor A Somorjai
The Fischer-Tropsch process, or the catalytic hydrogenation of carbon monoxide (CO), produces long chain hydrocarbons and offers an alternative to the use of crude oil for chemical feedstocks. The observed size dependence of cobalt (Co) catalysts for the Fischer-Tropsch reaction was studied with colloidally prepared Co nanoparticles and a chemical transient kinetics reactor capable of measurements under non-steady-state conditions. Co nanoparticles of 4.3 nm and 9.5 nm diameters were synthesized and tested under atmospheric pressure conditions and H2 /CO=2...
May 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28542579/transcriptomic-dissection-reveals-wide-spread-differential-expression-in-chickpea-during-early-time-points-of-fusarium-oxysporum-f-sp-ciceri-race-1-attack
#12
Sumanti Gupta, Anirban Bhar, Moniya Chatterjee, Amartya Ghosh, Sampa Das
Plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. Roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. Thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. Present study reported the molecular complexities in chickpea during Fusarium oxysporum f...
2017: PloS One
https://www.readbyqxmd.com/read/28539365/functional-analysis-of-human-cytochrome-p450-21a2-variants-involved-in-congenital-adrenal-hyperplasia
#13
Chunxue Wang, Pradeep S Pallan, Wei Zhang, Li Lei, Francis K Yoshimoto, Michael R Waterman, Martin Egli, F Peter Guengerich
Cytochrome P450 (P450, CYP) 21A2 is the major steroid 21-hydroxylase, converting progesterone to 11-deoxycorticosterone and 17α-hydroxy(OH)progesterone to 11-deoxycortisol. More than 100 CYP21A2 variants give rise to congenital adrenal hyperplasia (CAH). We previously reported a structure of wild-type (WT) human P450 21A2 with bound progesterone and now present a structure bound to the other substrate (17α-OH progesterone). We found that the 17α-OH progesterone- and progesterone-bound complex structures are highly similar, with only some minor differences in surface loop regions...
May 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28528957/proteasomes-in-corneal-epithelial-cells-and-cultured-autologous-oral-mucosal-epithelial-cell-sheet-caomecs-graft-used-for-the-ocular-surface-regeneration
#14
Fawzia Bardag-Gorce, Richard Hoft, Imara Meepe, Julio Garcia, Kumar Tiger, Andrew Wood, Amanda Laporte, Derek Pan, Andrew Makalinao, Robert Niihara, Joan Oliva, Arjie Florentino, Amber M Gorce, Jeremy Stark, Daileen Cortez, Samuel W French, Yutaka Niihara
PURPOSE: This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface. METHODS: Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface. RESULTS: LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR)...
May 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28499168/novel-imidazo-4-5-c-1-2-6-thiadiazine-2-2-dioxides-as-antiproliferative-trypanosoma-cruzi-drugs-computational-screening-from-neural-network-synthesis-and-in%C3%A2-vivo-biological-properties
#15
Angela Guerra, Pedro Gonzalez-Naranjo, Nuria E Campillo, Javier Varela, María L Lavaggi, Alicia Merlino, Hugo Cerecetto, Mercedes González, Alicia Gomez-Barrio, José A Escario, Cristina Fonseca-Berzal, Gloria Yaluf, Jorge Paniagua-Solis, Juan A Páez
A new family of imidazo[4,5-c][1,2,6]thiadiazine 2,2-dioxide with antiproliferative Trypanosoma cruzi properties was identified from a neural network model published by our group. The synthesis and evaluation of this new class of trypanocidal agents are described. These compounds inhibit the growth of Trypanosoma cruzi, comparable with benznidazole or nifurtimox. In vitro assays were performed to study their effects on the growth of the epimastigote form of the Tulahuen 2 strain, as well as the epimastigote and amastigote forms of CL clone B5 of Trypanosoma cruzi...
May 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28497908/the-molybdenum-cofactor-enzyme-marc-moonlighting-or-promiscuous-enzyme
#16
REVIEW
Angel Llamas, Alejandro Chamizo-Ampudia, Manuel Tejada-Jimenez, Aurora Galvan, Emilio Fernandez
Molybdenum (Mo) is present in the active center of eukaryotic enzymes as a tricyclic pyranopterin chelate compound forming the Mo Cofactor (Moco). Four Moco containing enzymes are known in eukaryotes, nitrate reductase (NR), sulfite oxidase (SO), xanthine oxidoreductase (XOR), and aldehyde oxidase (AO). A fifth Moco enzyme has been recently identified. Because of the ability of this enzyme to convert by reduction several amidoximes prodrugs into their active amino forms, it was named mARC (mitochondrial Amidoxime Reducing Component)...
May 12, 2017: BioFactors
https://www.readbyqxmd.com/read/28495463/development-of-an-animal-model-of-progressive-vaccinia-in-nu-nu-mice-and-the-use-of-bioluminescence-imaging-for-assessment-of-the-efficacy-of-monoclonal-antibodies-against-vaccinial-b5-and-l1-proteins
#17
Marina Zaitseva, Antonia Thomas, Clement A Meseda, Charles Y K Cheung, Claudia G Diaz, Yan Xiang, Shane Crotty, Hana Golding
Bioluminescence imaging (BLI) was used to follow dissemination of recombinant vaccinia virus (VACV) expressing luciferase (IHD-J-Luc) in BALB/c nu/nu mice treated post-challenge with monoclonal antibodies (MAbs) against L1 and B5 VACV proteins in a model of Progressive Vaccinia (PV). Areas Under the flux Curve (AUC) were calculated for viral loads in multiple organs in individual mice. Following scarification with 10(5) pfu, IHD-J-Luc VACV undergoes fast replication at the injection site and disseminates rapidly to the inguinal lymph nodes followed by spleen, liver, and axillary lymph nodes within 2-3 days and before primary lesions are visible at the site of scarification...
May 8, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28480969/epidemiological-and-genetic-analysis-concerning-the-non-enterovirus-71-and-non-coxsackievirus-a16-causative-agents-related-to-hand-foot-and-mouth-disease-in-anyang-city-henan-province-china-from-2011-to-2015
#18
Yang Li, Honghong Bao, Xiangping Zhang, Mingqiang Zhai, Xiaobing Bao, Demin Wang, Shuanhu Zhang
Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) are major pathogens of hand, foot and mouth disease (HFMD) and have been associated with consecutive outbreaks of HFMD in China over the past years. Although several other human enteroviruses (HEVs) have also acted as causative agents of HFMD, published information on their roles in the prevalence of HFMD is limited. This study was conducted to reveal the characteristics of the pathogenic spectrum and molecular epidemiology of the non-EV-71 and non-CV-A16 HEVs in Anyang City, which is located in north-central China and has a population of 5 million...
May 8, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28472682/insulin-enhances-the-peroxidase-activity-of-heme-by-forming-heme-insulin-complex-relevance-to-type-2-diabetes-mellitus
#19
Yi Huang, Zhen Yang, Huan Xu, Pengfei Zhang, Zhonghong Gao, Hailing Li
Evidences have implicated the involvement of heme in the type 2 diabetes mellitus (T2Dm) pathogenesis, but possible mediators linking between heme and diabetes are still poorly understood. Here, we explored a potential mechanism that linked heme, insulin and diabetes. Our results demonstrated the formation of heme-insulin complex by two classical methods, i.e. UV-vis and capillary electrophoresis-frontal analysis (CE-FA). UV-vis results implied heme binding insulin via bis-histidine sites, and CE-FA further revealed that, when insulin uses two sites binding with heme, this interaction occurs at high affinity (Kd=3...
May 1, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28448444/role-of-the-vanins-myeloperoxidase-axis-in-colorectal-carcinogenesis
#20
REVIEW
Francesco Mariani, Luca Roncucci
The presence of chronic inflammation in the colonic mucosa leads to an increased risk of cancer. Among proteins involved in the regulation of mucosal inflammation and that may contribute both to structural damage of the intestinal mucosa and to intestinal carcinogenesis, there are myeloperoxidase (MPO) and vanins. The infiltration of colonic mucosa by neutrophils may promote carcinogenesis through MPO, a key enzyme contained in the lysosomes of neutrophils that regulates local inflammation and the generation of reactive oxygen species (ROS) and mutagenic species...
April 27, 2017: International Journal of Molecular Sciences
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