keyword
https://read.qxmd.com/read/38493686/enhanced-antibacterial-activity-of-bovine-milk-exosome-based-drug-formulation-against-bacterial-pathogens
#1
JOURNAL ARTICLE
Qingjun Xu, Shuo Yang, Kai Zhang, Ying Liu, Lin Li, Shaoqi Qu
Milk is not only a source of nutrients, but also contains exosomes (Exo) that can serve as a vehicle for drug delivery. Here, we obtained bovine milk Exo using three efficient methods, demonstrating high quality for commercial production. The optimized Exo displayed a size of 105.2 nm and an entrapment efficiency of 88.4 %. The Exo has been functionalized with a combination therapy comprising isobavachalcone (IS) and polymyxin B (PB), referred to as IP-Exo. The antibacterial efficacy of IP-Exo was significantly enhanced, enabling the elimination of 99 % of multidrug-resistant (MDR) bacterial pathogens in 4 h...
March 15, 2024: Food Chemistry
https://read.qxmd.com/read/38473710/use-of-micrornas-as-diagnostic-prognostic-and-therapeutic-tools-for-glioblastoma
#2
REVIEW
David Valle-Garcia, Verónica Pérez de la Cruz, Itamar Flores, Aleli Salazar, Benjamín Pineda, Karla F Meza-Sosa
Glioblastoma (GB) is the most aggressive and common type of cancer within the central nervous system (CNS). Despite the vast knowledge of its physiopathology and histology, its etiology at the molecular level has not been completely understood. Thus, attaining a cure has not been possible yet and it remains one of the deadliest types of cancer. Usually, GB is diagnosed when some symptoms have already been presented by the patient. This diagnosis is commonly based on a physical exam and imaging studies, such as computed tomography (CT) and magnetic resonance imaging (MRI), together with or followed by a surgical biopsy...
February 20, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38468747/bovine-milk-derived-exosomes-affect-gut-microbiota-of-dss-induced-colitis-mice
#3
JOURNAL ARTICLE
Tonghao Li, Xiaolin Chen, Qien Qi, Xin Feng
The objective of this study was to investigate the effect of bovine milk derived exosomes (MDEs) on the gut microbiota of Dextran sodium sulfate (DSS)-induced colitis mice. Total of 42 specific pathogen free (SPF) male BALB/c mice (3 weeks old) were randomly assigned to three groups including control group, DSS group (DSS) and bovine milk derived exosome group (Exo), with 7 replicates/cages per treatment and two mice in one cage. 16S rRNA gene sequencing of cecal digesta samples was conducted. DSS significantly decreased the average daily feed intake of mice in DSS and Exo groups ( P  = 0...
March 2024: Indian Journal of Microbiology
https://read.qxmd.com/read/38452655/curcumin-encapsulated-in-milk-small-extracellular-vesicles-as-a-nanotherapeutic-alternative-in-experimental-chronic-liver-disease
#4
JOURNAL ARTICLE
Virginia Albaladejo-García, Laura Morán, Ana Santos-Coquillat, María I González, Hui Ye, Elena Vázquez Ogando, Javier Vaquero, Francisco Javier Cubero, Manuel Desco, Beatriz Salinas
Curcumin is a natural molecule widely tested in preclinical and clinical studies due to its antioxidant and anti-inflammatory activity. Nevertheless, its high hydrophobicity and low bioavailability limit in vivo applications. To overcome curcumin´s drawbacks, small extracellular vesicles (sEVs) have emerged as potential drug delivery systems due to their non-immunogenicity, nanometric size and amphiphilic composition. This work presents curcumin cargo into milk sEV structure and further in vitro and in vivo evaluation as a therapeutic nanoplatform...
March 6, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38414407/blockade-of-exosome-release-alleviates-the-hypersensitive-reaction-by-influencing-the-t-helper-cell-population-in-cow-s-milk-allergic-mice
#5
JOURNAL ARTICLE
Xin Ma, Jiaheng Xia, Jin Yuan, Xuanyi Meng, Hongbing Chen, Xin Li
The aim of this work was to evaluate the ameliorative effects of exosome biogenesis in cow's milk allergy (CMA) response. In this context, BALB/c mice were systemically sensitized with cow's milk proteins plus an aluminum adjuvant to induce CMA. The inhibitor GW4869 of exosome biogenesis was added before sensitization and then the anaphylactic reactions were evaluated both in vivo (clinical score and body temperature) and in vitro (serum histamine, allergen-specific antibodies, cytokines by ELISA and cell analysis by flow cytometry) to explore the role of exosomes in the development of CMA...
February 28, 2024: Food & Function
https://read.qxmd.com/read/38304545/characterization-of-exosomal-micrornas-in-preterm-infants-fed-with-breast-milk-and-infant-formula
#6
JOURNAL ARTICLE
Eun-Bit Kim, Jun Hwan Song, Linh Nguy-Hoang Le, Ho Kim, Ji Won Koh, Yekyeng Seo, Hwal Rim Jeong, Hyun-Taek Kim, Seongho Ryu
Breastfeeding not only reduces infection-related morbidity, but also increases growth of preterm infants. Advantages of breast milk (BM) for preterm infants are significant. They continue to be studied. However, because not all preterm infants can receive breastfeeding, bovine-based infant formula (IF) is used as an alternative, which may increase the risk of several preterm complications. Exosomes isolated from biofluids are emerging as biomarkers in research of various diseases. Here, we characterized miRNA contents of exosomes in urine and serum samples of preterm infants who were BM and IF fed and performed transcriptomic analysis of small RNA libraries...
2024: Frontiers in Nutrition
https://read.qxmd.com/read/38261277/cow-s-milk-allergy-may-induce-lipid-metabolism-disorder-in-balb-c-mice-via-exosomes
#7
JOURNAL ARTICLE
Xin Ma, Jiaheng Xia, Deming Gong, Zheling Zeng, Hongbing Chen, Xin Li
Allergic diseases and lipid-metabolism-disorder-derived diseases are both significant public health issues. Recent studies have shown that exosomes are associated with the course of allergic diseases and are involved in lipid metabolism. In this study, exosomes derived from cow's milk allergic (CMA) mice medially loaded lesser proteins favoring cholesterol metabolism. The levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-c) in the serum were increased in the CMA mice, and hepatic lipid deposition was observed in the liver, but these phenomena were improved by inhibiting the exosome release...
January 23, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38244155/human-milk-exosome-derived-circdnajb6-improves-bronchopulmonary-dysplasia-model-by-promoting-dnajb6-gene-transcription
#8
JOURNAL ARTICLE
Yubai Li, Boshi Yu, Huimin Li, Weiwei Hou, Jing Yin, Yahui Zhou, Zhangbin Yu
To verify the protective effect of circDNAJB6 on Bronchopulmonary dysplasia (BPD) cell and animal models and to explore the possible mechanism of its protective effect. The function of circDNAJB6 was investigated at the cell and animal levels. Nuclear and Cytoplasmic RNA extraction kits and fluorescence in situ hybridization (FISH) were used to explore the distribution of circDNAJB6 in cells, and the potential mechanism of circDNAJB6 was verified by q-PCR, luciferase assays and rescue experiments.CircDNAJB6 is abundant in breast milk exosomes...
January 20, 2024: Journal of Bioenergetics and Biomembranes
https://read.qxmd.com/read/38223538/oral-tnf-%C3%AE-sirna-delivery-via-milk-derived-exosomes-for-effective-treatment-of-inflammatory-bowel-disease
#9
JOURNAL ARTICLE
Geonhee Han, Hyosuk Kim, Hochung Jang, Eun Sun Kim, Sun Hwa Kim, Yoosoo Yang
Oral administration facilitates the direct delivery of drugs to lesions within the small intestine and colon, making it an ideal approach for treating patients with inflammatory bowel disease. However, multiple physical barriers impede the delivery of oral RNA drugs through the gastrointestinal tract. Herein, we developed a novel oral siRNA delivery system that protects nucleic acids in extreme environments by employing exosomes derived from milk to encapsulate tumor necrosis factor-alpha (TNF-α) siRNA completely...
April 2024: Bioactive Materials
https://read.qxmd.com/read/38219490/simple-methods-for-measuring-milk-exosomes-using-fluorescent-compound-gif-2250-2276
#10
JOURNAL ARTICLE
Saho Furukawa, Kyoka Kawaguchi, Kotomi Chikama, Ryohei Yamada, Yuji O Kamatari, Lee Wah Lim, Hiroko Koyama, Yasuo Inoshima, Mitsushi J Ikemoto, Saishi Yoshida, Yoko Hirata, Kyoji Furuta, Hiroshi Takemori
Exosomes are small extracellular vesicles (EVs) found in culture supernatants, blood, and breast milk. The size of these nanocomplexes limits the methods of EV analyses. In this study, nitrobenzoxadiazole (NBD), a fluorophore, conjugated endosome-lysosome imager, GIF-2250 and its derivative, GIF-2276, were evaluated for exosome analyses. A correlation was established between GIF-2250 intensity and protein maker levels in bovine milk exosomes. We found that high-temperature sterilization milk may not contain intact exosomes...
January 9, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38218063/review-of-exosomes-and-their-potential-for-veterinary-medicine
#11
REVIEW
Mohammad Heidarpour, Mark Krockenberger, Peter Bennett
Small extracellular vesicles called exosomes are released by almost all cell types and play a crucial role in both healthy and pathological circumstances. Exosomes, found in biological fluids (including plasma, urine, milk, semen, saliva, abdominal fluid and cervical vaginal fluid) and ranging in size from 50 to 150 nm, are critical for intercellular communication. Analysis of exosomal cargos, including micro RNAs (miRNAs), proteins and lipids, has been proposed as valuable diagnostic and prognostic biomarkers of disease...
January 8, 2024: Research in Veterinary Science
https://read.qxmd.com/read/38203844/breed-related-differential-microrna-expression-and-analysis-of-colostrum-and-mature-milk-exosomes-in-bamei-and-landrace-pigs
#12
JOURNAL ARTICLE
Jie Li, Xuefeng Shang, Sen Zhang, Qiaoli Yang, Zunqiang Yan, Pengfei Wang, Xiaoli Gao, Shuangbao Gun, Xiaoyu Huang
Breast milk, an indispensable source of immunological and nutrient components, is essential for the growth and development of newborn mammals. MicroRNAs (miRNAs) are present in various tissues and body fluids and are selectively packaged inside exosomes, a type of membrane vesicle. Milk exosomes have potential regulatory effects on the growth, development, and immunity of newborn piglets. To explore the differences in milk exosomes related to the breed and milk type, we isolated exosomes from colostrum and mature milk from domestic Bamei pigs and foreign Landrace pigs by using density gradient centrifugation and then characterized them by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA)...
January 4, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38184235/reprogramming-tumor-associated-macrophages-by-a-dually-targeted-mexo-system-as-a-potent-monotherapy-for-cancer
#13
JOURNAL ARTICLE
Ying Chen, Liang Gong, Yulin Cao, Zhiang Liu, Yuanben Wang, Han Cheng, Yuyang Feng, Surui Yao, Yuan Yin, Zhimeng Wu, Zhaohui Huang
Tumor-associated macrophages (TAMs) play a key role in inducing an immunosuppressive tumor microenvironment (TME) and cancer immune escape. We previously revealed that PDL1 (a key immune checkpoint) was upregulated in TAMs and induced M2 polarization, highlighting PDL1 in TAMs as a promising cancer therapeutic target. In this study, we developed an engineered milk exosome (mExo) system decorated with M2pep (an M2 macrophage binding peptide) and 7D12 (an anti-EGFR nanobody) (7D12-mExo-M2pep-siPDL1) to specifically deliver siPDL1 into M2 TAMs...
January 4, 2024: Journal of Controlled Release
https://read.qxmd.com/read/38165514/milk-exosomal-microrna-profiling-identified-mir-375-and-mir-199-5p-for-regulation-of-immune-response-during-subclinical-mastitis-of-crossbred-cattle
#14
JOURNAL ARTICLE
Sudarshan Mahala, Amit Kumar, Hari Om Pandey, Shikha Saxena, Shivani Khanna, Manoj Kumar, Deepak Kumar, Ujjwal Kumar De, Ashwni Kumar Pandey, Triveni Dutt
BACKGROUND: The dairy industry has experienced significant economic losses as a result of mastitis, an inflammatory disease of cows, including both subclinical and clinical cases. Milk exosome microRNAs have gained attention due to their stable and selective wrapping nature, offering potential for the prognosis and diagnosis of bovine mastitis, the most common pathological condition of the mammary gland. METHODS AND RESULTS:  In the present investigation, the microRNA profile of milk exosomes was explored using high-throughput small RNA sequencing data in sub-clinical mastitic and healthy crossbred Vrindavani cattle...
January 2, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38164738/exosome-delivered-mir-410-3p-reverses-epithelial-mesenchymal-transition-migration-and-invasion-of-trophoblasts-in-spontaneous-abortion
#15
JOURNAL ARTICLE
Zhen-Yue Chen, Zhen Li, Yun Zong, Bo Xia, Song-Ping Luo, Gao-Pi Deng, Jie Gao
Current studies have indicated that insufficient trophoblast epithelial-mesenchymal transition (EMT), migration and invasion are crucial for spontaneous abortion (SA) occurrence and development. Exosomal miRNAs play significant roles in embryonic development and cellular communication. Hereon, we explored the roles of serum exosomes derived from SA patients on trophoblast EMT, migration and invasion. Exosomes were isolated from normal control (NC) patients with abortion for unplanned pregnancy and SA patients, then characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting...
January 2, 2024: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/38115131/potential-therapeutic-effects-of-milk-derived-exosomes-on-intestinal-diseases
#16
REVIEW
Zhifu Cui, Felix Kwame Amevor, Xingtao Zhao, Chunyan Mou, Jiaman Pang, Xie Peng, Anfang Liu, Xi Lan, Lingbin Liu
Exosomes are extracellular vesicles with the diameter of 30 ~ 150 nm, and are widely involved in intercellular communication, disease diagnosis and drug delivery carriers for targeted disease therapy. Therapeutic application of exosomes as drug carriers is limited due to the lack of sources and methods for obtaining adequate exosomes. Milk contains abundant exosomes, several studies have shown that milk-derived exosomes play crucial roles in preventing and treating intestinal diseases. In this review, we summarized the biogenesis, secretion and structure, current novel methods used for the extraction and identification of exosomes, as well as discussed the role of milk-derived exosomes in treating intestinal diseases, such as inflammatory bowel disease, necrotizing enterocolitis, colorectal cancer, and intestinal ischemia and reperfusion injury by regulating intestinal immune homeostasis, restoring gut microbiota composition and improving intestinal structure and integrity, alleviating conditions such as oxidative stress, cell apoptosis and inflammation, and reducing mitochondrial reactive oxygen species (ROS) and lysosome accumulation in both humans and animals...
December 20, 2023: Journal of Nanobiotechnology
https://read.qxmd.com/read/38105431/novel-roles-of-bovine-milk-derived-exosomes-in-skin-antiaging
#17
JOURNAL ARTICLE
Lu Lu, Wei Bai, Miao Wang, Chunle Han, Huanqing Du, Na Wang, Mengya Gao, Dan Li, Fengwei Dong, Xiaohu Ge
BACKGROUND: Exosomes are small vesicles released from cells and are found in various mammalian biological fluids, such as bovine milk, which has been employed in skincare for many years, apart from its dairy applications. In addition, exosomes have been recognized as vehicles for intercellular communication. AIMS: In this study, we aimed to investigate the novel effects of bovine milk-derived exosomes (MK-Exo) on antiaging in human skin. METHODS: Initially, MK-Exo were co-cultured with keratinocytes and fibroblasts; subsequent analysis involved qPCR and western blotting to assess induced gene expression...
December 17, 2023: Journal of Cosmetic Dermatology
https://read.qxmd.com/read/38052991/porcine-milk-exosomes-modulate-the-immune-functions-of-cd14-monocytes-in-vitro
#18
JOURNAL ARTICLE
Gabriela Ávila Morales, Daria De Leonardis, Joel Filipe, Rafaela Furioso Ferreira, Alessandro Agazzi, Helga Sauerwein, Marcello Comi, Vladimir Mrljak, Cristina Lecchi, Fabrizio Ceciliani
Exosomes mediate near and long-distance intercellular communication by transferring their molecular cargo to recipient cells, altering their biological response. Milk exosomes (MEx) are internalized by immune cells and exert immunomodulatory functions in vitro. Porcine MEx can accumulate in the small intestine, rich in macrophages. No information is available on the immunomodulatory ability of porcine MEx on porcine monocytes, which are known precursors of gut macrophages. Therefore, this study aims at (1) assessing the in vitro uptake of porcine MEx by porcine monocytes (CD14+), and (2) evaluating the in vitro impact of porcine MEx on porcine monocytes immune functions...
December 5, 2023: Scientific Reports
https://read.qxmd.com/read/38047368/milk-exosomes-anchored-with-hydrophilic-and-zwitterionic-motifs-enhance-mucus-permeability-for-applications-in-oral-gene-delivery
#19
JOURNAL ARTICLE
Chenzhen Zhang, Hengli Zhang, Héctor A Millán Cotto, Timothy L Boyer, Matthew R Warren, Chia-Ming Wang, Joshua Luchan, Pradeep K Dhal, Rebecca L Carrier, Ambika G Bajpayee
Exosomes have emerged as a promising tool for the delivery of drugs and genetic materials, owing to their biocompatibility and non-immunogenic nature. However, challenges persist in achieving successful oral delivery due to their susceptibility to degradation in the harsh gastrointestinal (GI) environment and impeded transport across the mucus-epithelium barrier. To overcome these challenges, we have developed high-purity bovine milk exosomes (mExo) as a scalable and efficient oral drug delivery system, which can be customized by incorporating hydrophilic and zwitterionic motifs on their surface...
December 4, 2023: Biomaterials Science
https://read.qxmd.com/read/38031117/post-insertion-technique-to-introduce-targeting-moieties-in-milk-exosomes-for-targeted-drug-delivery
#20
JOURNAL ARTICLE
Hochung Jang, Hyosuk Kim, Eun Hye Kim, Geonhee Han, Yeongji Jang, Yelee Kim, Jong Won Lee, Sang Chul Shin, Eunice EunKyeong Kim, Sun Hwa Kim, Yoosoo Yang
BACKGROUND: Recently, increased attention has been given on exosomes as ideal nanocarriers of drugs owing to their intrinsic properties that facilitate the transport of biomolecular cargos. However, large-scale exosome production remains a major challenge in the clinical application of exosome-based drug delivery systems. Considering its biocompatibility and stability, bovine milk is a suitable natural source for large-scale and stable exosome production. Because the active-targeting ability of drug carriers is essential to maximize therapeutic efficacy and minimize side effects, precise membrane functionalization strategies are required to enable tissue-specific delivery of milk exosomes with difficulty in post-isolation modification...
November 29, 2023: Biomaterials Research
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