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https://www.readbyqxmd.com/read/28731265/application-of-dead-end-knockout-zebrafish-as-recipients-of-germ-cell-transplantation
#1
Qian Li, Wataru Fujii, Kunihiko Naito, Goro Yoshizaki
Germ cell transplantation is a promising technology for the propagation of endangered or valuable fishes. In this technique, sterile male and female recipient fish are injected with donor germ cells so they can produce viable gametes derived from the donor cells. The dead end (dnd) gene is involved in the migration of primordial germ cells; therefore, dnd-knockout zebrafish are expected to be germ-cell-free, making them suitable recipients for germ cell transplantation. dnd mutants were produced by microinjecting 2 nl of 10 ng/μl cRNAs encoding zinc finger nucleases against dnd into the blastodisc of zebrafish embryos before the cell cleavage stage...
July 21, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28712340/deep-sequencing-reveals-the-mitochondrial-dna-variation-landscapes-of-breast-to-brain-metastasis-blood-samples
#2
Rhiannon E McGeehan, Lewis A Cockram, D Timothy J Littlewood, Kathleen Keatley, Diana M Eccles, Qian An
Breast-to-brain metastasis (BBM) often represents a terminal event, due to the inability of many systemic treatments to cross the blood-brain barrier (BBB), rendering the brain a sanctuary site for tumour cells. Identifying genetic variations that can predict the patients who will develop BBM would allow targeting of adjuvant treatments to reduce risk while disease bulk is minimal. Germ-line genetic variations may contribute to whether a BBM forms by influencing the primary tumour subtype that presents, or by influencing the host response to the tumour or treatment regimen, or by facilitating transition of tumour cells across the BBB and establish a viable brain metastasis...
July 15, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28711945/the-molecular-characterization-of-porcine-egg-precursor-cells
#3
Te-Sha Tsai, Jacqueline Johnson, Yvonne White, Justin C St John
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. Egg precursor cells (EPCs), isolated from the ovarian cortex, have the potential to be used to overcome female infertility. We aimed to define the origins of EPCs by analyzing their gene expression profiles and mtDNA content using a mini-pig model. We characterized FAC-sorted DDX4+-derived porcine EPCs by performing RNA-sequencing and determined that they utilize pathways important for cell cycle and proliferation, which supports the existence of adult mitotically active oogonial cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28694164/a-homologue-of-nr5a1-activates-cyp19a1a-transcription-additively-with-nr5a2-in-ovarian-follicular-cells-of-the-orange-spotted-grouper
#4
Boyang Shi, Huijie Lu, Lihong Zhang, Weimin Zhang
Transcription factors of nuclear receptor 5A (Nr5a) subfamily play pivotal roles in regulation of steroidogenic enzymes in vertebrates including teleosts. In the orange-spotted grouper, the expression of Nr5a1a was only detectable in the ovary, spleen, and head kidney in the female. The immunoreactive Nr5a1a was present in ovarian follicular and germ cells. In the ovarian follicular cells surrounding vitellogenic oocytes, Nr5a1a was detected both in the nucleus and cytoplasm, and co-localized with Cyp19a1a and Nr5a2...
July 8, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28691729/in-vitro-differentiation-of-primordial-germ-cells-and-oocyte-like-cells-from-stem-cells
#5
REVIEW
José J N Costa, Glaucinete B Souza, Maria A A Soares, Regislane P Ribeiro, Robert van den Hurk, José R V Silva
Infertility is the result of failure due to an organic disorder of the reproductive organs, especially their gametes. Recently, much progress has been made on generating germ cells, including oocytes, from various types of stem cells. This review focuses on advances in female germ cell differentiation from different kinds of stem cells, with emphasis on embryonic stem cells, adult stem cells, and induced pluripotent stem cells. The advantages and disadvantages of the derivation of female germ cells from several types of stem cells are also highlighted, as well as the ability of stem cells to generate mature and functional female gametes...
July 10, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28691222/the-morphology-of-the-male-reproductive-system-spermatogenesis-and-the-spermatozoon-of-daphnia-magna-crustacea-branchiopoda
#6
Maggie Wuerz, Erwin Huebner, Judith Huebner
This study analyses the histological and cellular morphology of the testis and sperm development in the male Daphnia magna Straus 1820. Due to the rarity of males and predominately parthenogenetic lifecycle of Daphnia, there has been limited detailed information on males in contrast to the well-studied female. Using light and electron microscopy approaches, we describe the morphology of the testis during the progression from an immature to mature testis. The testis has an encasing muscular mesh sheath outside the basal lamina, beneath which is a thin somatic epithelial cell layer...
July 10, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28676390/effects-of-bisphenol-a-analogues-on-reproductive-functions-in-mice
#7
Mingxin Shi, Nikola Sekulovski, James A MacLean, Kanako Hayashi
This study was performed to examine whether bisphenol (BP) A analogues, BPE and BPS, negatively impacts reproductive functions using mice as a model. CD-1 mice were exposed to control treatment (corn oil), BPA, BPE and BPS (50μg/kg or 10mg/kg) from birth to postnatal day (PND) 60 by s.c. injection every three days. Sperm counts or motility was significantly reduced by BPA, BPE or BPS exposure on PND 60 or PND 90. Exposure of BPA, BPE and BPS disrupted the progression of germ cell development, as morphometric analyses exhibited an abnormal distribution of the stages of spermatogenesis...
July 1, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28669362/putative-germline-and-pluripotent-stem-cells-in-adult-mouse-ovary-and-their-in-vitro-differentiation-potential-into-oocyte-like-and-somatic-cells
#8
Yashar Esmaeilian, Arzu Atalay, Esra Erdemli
According to classical knowledge of reproductive biology, in the ovary of female mammals there is a limited number of oocytes and there is no possibility of renewal if the oocytes are lost due to disease or injury. However, in recent years, the results of some studies on renewal and formation of oocytes and follicles in the adult mammalian ovary have led to the questioning of this opinion. The aim of our study is to demonstrate the presence of putative germline and pluripotent stem cells in the adult mouse ovary and their differentiation potential into germ and somatic cells...
July 3, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28664453/the-clinical-impact-of-18-f-fdg-pet-ct-in-extracranial-pediatric-germ-cell-tumors
#9
Adam Hart, Reza Vali, Eman Marie, Furqan Shaikh, Amer Shammas
BACKGROUND: Extracranial germ cell tumors are an uncommon pediatric malignancy with limited information on the clinical impact of (18)F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) in the literature. OBJECTIVE: The purpose of this study was to evaluate and compare the clinical impact on management of (18)F-FDG PET/CT with diagnostic computed tomography (CT) in pediatric extracranial germ cell tumor. MATERIALS AND METHODS: The list of (18)F-FDG PET/CT performed for extracranial germ cell tumor between May 2007 and November 2015 was obtained from the nuclear medicine database...
June 29, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28662518/effects-of-n-nitrosodiethylamine-a-potent-carcinogen-on-sexual-development-gametogenesis-and-oocyte-maturation
#10
Reshma Nair U, Anju C Victor, Vinod Paul, Bindhu Paul-Prasanth
N-Nitrosodiethylamine (DEN), a well-known hepatocarcinogen, is found in certain food products as such or as a metabolic byproduct. This study investigated the effects of DEN on sexual development, gametogenesis, and oocyte maturation in Japanese medaka (Oryzias latipes). DEN reduced the germ cell number dose-dependently during early stages of sexual differentiation in XX larvae, resulting in underdeveloped ovaries in adulthood at low doses. This effect was sex-specific as no such changes were seen in XY larvae...
2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/28658171/gonadal-germ-cell-tumors-in-children-a-retrospective-review-of-a-10-year-single-center-experience
#11
Xiaokun Lin, Dazhou Wu, Na Zheng, Qiongzhang Xia, Yijiang Han
BACKGROUND: The true incidence of gonadal germ cell tumors (GCTs) in children is unknown. Few studies have been published concerning about pediatric gonadal GCTs. The aim of this study is to review and analyze clinical data on the diagnosis and management of gonadal GCTs in children. METHODS: Between 2005 and 2015, 127 pediatric patients (<14 years old) with gonadal GCTs admitted to our institute were reviewed. Clinical features, imaging and laboratory studies, surgical approaches, as well as pathological diagnoses were recorded...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28656118/pediatric-ovarian-growing-teratoma-syndrome
#12
Rebecca M Rentea, Aaron Varghese, Atif Ahmed, Alexander Kats, Michelle Manalang, Tazim Dowlut-McElroy, Richard J Hendrickson
Ovarian immature teratoma is a germ cell tumor that comprises less than 1% of ovarian cancers and is treated with surgical debulking and chemotherapy depending on stage. Growing teratoma syndrome (GTS) is the phenomenon of the growth of mature teratoma elements with normal tumor markers during or following chemotherapy for treatment of a malignant germ cell tumor. These tumors are associated with significant morbidity and mortality due to invasive and compressive growth as well as potential for malignant transformation...
2017: Case Reports in Surgery
https://www.readbyqxmd.com/read/28655646/vitamin-d-deficiency-impairs-testicular-development-and-spermatogenesis-in-mice
#13
Lin Fu, Yuan-Hua Chen, Shen Xu, Yan-Li Ji, Cheng Zhang, Hua Wang, De-Xin Yu, De-Xiang Xu
Vitamin D deficiency is prevalent especially in men. Nevertheless, whether vitamin D deficiency impairs male reproduction remains under debate. The aim of this study is to investigate whether vitamin D deficiency has an impact on testicular development and spermatogenesis in mice. In the control group and vitamin D deficient (VDD) diet group, dams and their pups were fed with standard-chow diet and VDD diet, respectively. Interestingly, testicular weight and sperm quality are reduced, testicular germ cell proliferation is suppressed, and the percentage of mature seminiferous tubules is decreased in VDD diet-fed mice...
June 24, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28648994/dynamic-and-differential-expression-of-the-gonadal-aromatase-during-the-process-of-sexual-differentiation-in-a-novel-transgenic-cyp19a1a-egfp-zebrafish-line
#14
Nathalie Hinfray, Frédéric Sohm, Morgane Caulier, Edith Chadili, Benjamin Piccini, Camille Torchy, Jean-Marc Porcher, Yann Guiguen, François Brion
In zebrafish, there exists a clear need for new tools to study sex differentiation dynamic and its perturbation by endocrine disrupting chemicals. In this context, we developed and characterized a novel transgenic zebrafish line expressing green fluorescent protein (GFP) under the control of the zebrafish cyp19a1a (gonadal aromatase) promoter. In most gonochoristic fish species including zebrafish, cyp19a1a, the enzyme responsible for the synthesis of estrogens, has been shown to play a critical role in the processes of reproduction and sexual differentiation...
June 22, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28619824/setd1b-encoding-a-histone-3-lysine-4-methyltransferase-is-a-maternal-effect-gene-required-for-the-oogenic-gene-expression-program
#15
David Brici, Qinyu Zhang, Susanne Reinhardt, Andreas Dahl, Hella Hartmann, Kerstin Schmidt, Neha Goveas, Jiahao Huang, Lenka Gahurova, Gavin Kelsey, Konstantinos Anastassiadis, A Francis Stewart, Andrea Kranz
Germ cell development involves major reprogramming of the epigenome to prime the zygote for totipotency. Histone 3 lysine 4 (H3K4) methylations are universal epigenetic marks mediated in mammals by six H3K4 methyltransferases related to fly Trithorax, including two yeast Set1 orthologs: Setd1a and Setd1b. Whereas Setd1a plays no role in oogenesis, we report that Setd1b deficiency causes female sterility in mice. Oocyte-specific Gdf9-iCre conditional knockout (Setd1b(Gdf9) cKO) ovaries develop through all stages; however, follicular loss accumulated with age and unfertilized metaphase II (MII) oocytes exhibited irregularities of the zona pellucida and meiotic spindle...
July 15, 2017: Development
https://www.readbyqxmd.com/read/28614211/mixed-gonadal-germ-cell-tumor-composed-of-a-spermatocytic-tumor-like-component-and-germinoma-arising-in-gonadoblastoma-in-a-phenotypic-woman-with-a-46-xx-peripheral-karyotype-report-of-the-first-case
#16
Alejandro A Gru, Eli S Williams, Dengfeng Cao
We report a unique case of gonadal mixed germ cell tumor (GCT) composed of a predominantly spermatocytic tumor (ST)-like component and a minor component of germinoma arising in gonadoblastoma in a phenotypic woman with a 46, XX peripheral karotype. The patient was a 24-year-old woman (gravida 2, para 1) found to have a 7 cm pelvic mass during routine obstetric ultrasound examination at 20 weeks gestational age. She underwent a left salpingo-gonadectomy at gestational age 23 and 2/7 weeks. She recovered well and delivered a healthy baby at full term...
June 13, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28612220/individual-outcome-prediction-for-myelodysplastic-syndrome-mds-and-secondary-acute-myeloid-leukemia-from-mds-after-allogeneic-hematopoietic-cell-transplantation
#17
Michael Heuser, Razif Gabdoulline, Patrick Löffeld, Vera Dobbernack, Henriette Kreimeyer, Mira Pankratz, Madita Flintrop, Alessandro Liebich, Sabrina Klesse, Victoria Panagiota, Michael Stadler, Martin Wichmann, Rabia Shahswar, Uwe Platzbecker, Christian Thiede, Thomas Schroeder, Guido Kobbe, Robert Geffers, Brigitte Schlegelberger, Gudrun Göhring, Hans-Heinrich Kreipe, Ulrich Germing, Arnold Ganser, Nicolaus Kröger, Christian Koenecke, Felicitas Thol
We integrated molecular data with available prognostic factors in patients undergoing allogeneic hematopoietic cell transplantation (alloHCT) for myelodysplastic syndrome (MDS) or secondary acute myeloid leukemia (sAML) from MDS to evaluate their impact on prognosis. Three hundred four patients were sequenced for mutations in 54 genes. We used a Cox multivariate model and competing risk analysis with internal and cross validation to identify factors prognostic of overall survival (OS), cumulative incidence of relapse (CIR), and non-relapse mortality (NRM)...
August 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28609271/viability-of-homologous-and-heterologous-subcutaneous-transplantation-of-fresh-germ-cells-in-rabbits
#18
Paula M A Cerialle, Carlos G Almodin, Moacir R M Radaelli, Vânia C Minguetti-Câmara, Michelle C Souza, Carlos A M Oliveira, Antonio J Gonçalves
OBJECTIVE: This study aimed to compare heterologous to homologous transplantation of fresh ovarian germ cells in rabbits. METHODS: Twelve female white New Zealand rabbits (Oryctolagus cuniculus) were randomly numbered and submitted to bilateral oophorectomies. The ovaries from the six odd-numbered rabbits were dissected and cortical germinal tissue was digested in collagenase type 1 to obtain six solutions containing stromal and germ cells, which were injected in the abdominal region of the odd-numbered rabbits themselves (homologous transplantation) and of the even-numbered rabbits (heterologous transplantation) off immunosuppression...
June 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28601505/heat-and-chemical-treatments-in-adult-cyprinus-carpio-pisces-cypriniformes-rapidly-produce-sterile-gonads
#19
Sullip Kumar Majhi, Avinash Rambhau Rasal, Basdeo Kushwaha, Sudhir Raizada
Several options have been proposed for eradication of germ cells (GCs) in mammals such as treatment with cytotoxic drugs, irradiation, cold ischemia and hyperthermic treatment. Some of these methods have been also tried in fish but conditions for complete sterilisation of gonads have not been established. Here, we report the production of sterile adult common carp Cyprinus carpio in 10 weeks by the heat and chemical treatments. The cytotoxic drug busulfan (40mg/kg) was intraperitoneally injected into the animals at 2-week intervals (5 doses in total), and they were maintained in water at 38°C between Weeks 1 and 10...
June 3, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28601145/ovarian-development-and-the-reproductive-profile-of-astyanax-altiparanae-teleostei-characidae-over-one-year-applications-in-fish-farming
#20
Mônica Cassel, Chayrra Chehade, Giovana Souza Branco, Danilo Caneppele, Elizabeth Romagosa, Maria Inês Borella
Astyanax altiparanae is a Brazilian species of substantial commercial, environmental and scientific importance; however, existing studies on its reproduction do not seem to provide enough details. In light of the increasing use of this species in fish farming and the need for basic studies for the development of new production technologies, we describe the structural and ultrastructural characteristics of the ovaries of A. altiparanae, and characterize the species' reproductive cycle. Females were collected monthly from March 2013 to February 2014, and reproductive management began in October 2013...
August 2017: Theriogenology
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