Thomas Daphnis, Benjamin Tomasetti, Vincent Delmez, Kevin Vanvarenberg, Véronique Préat, Charlotte Thieffry, Patrick Henriet, Christine Dupont-Gillain, Arnaud Delcorte
The potential of mass spectrometry imaging, and especially ToF-SIMS 2D and 3D imaging, for submicrometer-scale, label-free molecular localization in biological tissues is undisputable. Nevertheless, sensitivity issues remain, especially when one wants to achieve the best lateral and vertical (nanometer-scale) resolution. In this study, the interest of in situ matrix transfer for tissue analysis with cluster ion beams (Bin + , Arn + ) is explored in detail, using a series of six low molecular weight acidic (MALDI) matrices...
September 15, 2023: Journal of the American Society for Mass Spectrometry