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https://www.readbyqxmd.com/read/29342982/detection-of-zro%C3%A2-nanoparticles-in-lung-tissue-sections-by-time-of-flight-secondary-ion-mass-spectrometry-and-ion-beam-microscopy
#1
Lothar Veith, Julia Böttner, Antje Vennemann, Daniel Breitenstein, Carsten Engelhard, Jan Meijer, Irina Estrela-Lopis, Martin Wiemann, Birgit Hagenhoff
The increasing use of nanoparticles (NP) in commercial products requires elaborated techniques to detect NP in the tissue of exposed organisms. However, due to the low amount of material, the detection and exact localization of NP within tissue sections is demanding. In this respect, Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and Ion Beam Microscopy (IBM) are promising techniques, because they both offer sub-micron lateral resolutions along with high sensitivities. Here, we compare the performance of the non-material-consumptive IBM and material-consumptive ToF-SIMS for the detection of ZrO₂ NP (primary size 9-10 nm) in rat lung tissue...
January 15, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29311645/author-correction-fluorescent-d-amino-acids-reveal-bi-cellular-cell-wall-modifications-important-for-bdellovibrio-bacteriovorus-predation
#2
Erkin Kuru, Carey Lambert, Jonathan Rittichier, Rob Till, Adrien Ducret, Adeline Derouaux, Joe Gray, Jacob Biboy, Waldemar Vollmer, Michael VanNieuwenhze, Yves V Brun, R Elizabeth Sockett
In the original version of this Article, a grant number and acknowledgement were omitted. The Acknowledgements section should have stated that one of the 3D SIM microscopes used for this research was supported by Medical Research Council UK grant (MR/K015753/1) to S. Foster, University of Sheffield, UK, and that the authors thank C. Walther and S. Foster for the access and their kind help with this. This has now been corrected in all versions of the Article.
January 8, 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29311421/size-of-graphene-sheets-determines-the-structural-and-mechanical-properties-of-3d-graphene-foams
#3
Zhiqiang Shen, Huilin Ye, Chi Zhou, Martin Kroeger, Ying Li
Graphene has been recognized as emerging 2D nanomaterial for many applications. Assembly of graphene sheets into 3D structures is an attractive way to enable their macroscopic applications and preserve the exceptional mechanical and physical properties of their constituents. In this study, we develop a coarse-grained (CG) model for 3D graphene foams (GFs) based on the CG model for a 2D graphene sheet by Ruiz et al.\ [Carbon 82:103--115, 2015]. We find that the sizes of graphene sheets play an important role in both structural and mechanical properties of 3D GFs...
January 9, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29295614/insights-into-the-maldi-process-after-matrix-deposition-by-sublimation-using-3d-tof-sims-imaging
#4
Sebastiaan Van Nuffel, Nicolas Elie, Ethan Yang, Julius Nouet, David Touboul, Pierre Chaurand, Alain Brunelle
Imaging mass spectrometry (IMS) has become a powerful tool to characterise the spatial distribution of biomolecules in thin tissue sections. In the case of matrix-assisted laser desorption ionisation (MALDI) IMS, homogeneous matrix deposition is critical to produce high quality ion images and sublimation in particular has shown to be an excellent matrix deposition method for the imaging of lipids. Matrix deposition by sublimation is, however, a completely solvent-free system, which ought to prevent the mixing of matrix and analytes thought to be necessary for successful MALDI...
January 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29245793/three-dimensional-live-multi-label-light-sheet-imaging-with-synchronous-excitation-multiplexed-structured-illumination
#5
Dongli Xu, Weibin Zhou, Leilei Peng
Multiplexed imaging is a powerful tool for studying complex interactions inside biological systems. Spectral imaging methods that capture multiple fluorescent markers synchronously without sacrificing the imaging speed or resolution are most suitable for live imaging. We describe spectral-encoded structured illumination (spectral-SIM) light-sheet microscopy, which enables parallel multi-excitation-channel imaging in 3D. Spectral-SIM encodes the excitation wavelength as the phase of the illumination pattern, and allows synchronous image capture over multiple excitation channels at the same speed and spatial resolution as mono-channel structured light-sheet imaging...
December 11, 2017: Optics Express
https://www.readbyqxmd.com/read/29241145/wet-chemical-etching-of-atom-probe-tips-for-artefact-free-analyses-of-nanoscaled-semiconductor-structures
#6
D Melkonyan, C Fleischmann, A Veloso, A Franquet, J Bogdanowicz, R J H Morris, W Vandervorst
We introduce an innovative specimen preparation method employing the selectivity of a wet-chemical etching step to improve data quality and success rates in the atom probe analysis of contemporary semiconductor devices. Firstly, on the example of an SiGe fin embedded in SiO2 we demonstrate how the selective removal of SiO2 from the final APT specimen significantly improves accuracy and reliability of the reconstructed data. With the oxide removal, we eliminate the origin of shape artefacts, i.e. the formation of a non-hemispherical tip shape, that are typically observed in the reconstructed volume of complex systems...
December 7, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/29192283/chemical-changes-in-layered-ferroelectric-semiconductors-induced-by-helium-ion-beam
#7
Alex Belianinov, Matthew J Burch, Holland E Hysmith, Anton V Ievlev, Vighter Iberi, Michael A Susner, Michael A McGuire, Peter Maksymovych, Marius Chyasnavichyus, Stephen Jesse, Olga S Ovchinnikova
Multi-material systems interfaced with 2D materials, or entirely new 3D heterostructures can lead to the next generation multi-functional device architectures. Physical and chemical control at the nanoscale is also necessary tailor these materials as functional structures approach physical limit. 2D transition metal thiophosphates (TPS), with a general formulae Cu1-xIn1+x/3P2S6, have shown ferroelectric polarization behavior with a T c above the room temperature, making them attractive candidates for designing both: chemical and physical properties...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29149722/effects-of-middle-ear-quasi-static-stiffness-on-sound-transmission-quantified-by-a-novel-3-axis-optical-force-sensor
#8
Ivo Dobrev, Jae Hoon Sim, Baktash Aqtashi, Alexander M Huber, Thomas Linder, Christof Röösli
BACKGROUND: Intra-operative quantification of the ossicle mobility could provide valuable feedback for the current status of the patient's conductive hearing. However, current methods for evaluation of middle ear mobility are mostly limited to the surgeon's subjective impression through manual palpation of the ossicles. This study investigates how middle ear transfer function is affected by stapes quasi-static stiffness of the ossicular chain. The stiffness of the middle ear is induced by a) using a novel fiber-optic 3-axis force sensor to quantify the quasi-static stiffness of the middle ear, and b) by artificial reduction of stapes mobility due to drying of the middle ear...
November 10, 2017: Hearing Research
https://www.readbyqxmd.com/read/29138765/sizing-sub-diffraction-limit-electrosprayed-droplets-by-structured-illumination-microscopy
#9
Adam Hollerbach, David Logsdon, Kiran Iyer, Anyin Li, J Andy Schaber, R Graham Cooks
Electrosprayed droplets are widely studied for their role in the formation of ions at atmospheric pressure. Most droplet measurement methods used today employ light scattering to infer information about an electrosprayed droplet's size. However, these methods fail to measure droplets smaller than about 400 nm in diameter due to constraints imposed by the diffraction limit of light. To overcome this limitation, a super resolution fluorescence microscopy-based method for determining the sizes of electrosprayed droplets has been developed...
November 15, 2017: Analyst
https://www.readbyqxmd.com/read/29131162/the-3d-orbisims-label-free-metabolic-imaging-with-subcellular-lateral-resolution-and-high-mass-resolving-power
#10
Melissa K Passarelli, Alexander Pirkl, Rudolf Moellers, Dmitry Grinfeld, Felix Kollmer, Rasmus Havelund, Carla F Newman, Peter S Marshall, Henrik Arlinghaus, Morgan R Alexander, Andy West, Stevan Horning, Ewald Niehuis, Alexander Makarov, Colin T Dollery, Ian S Gilmore
We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; under 200 nm for inorganic species and under 2 μm for biomolecules) with the high mass-resolving power of an Orbitrap (>240,000 at m/z 200). This allows exogenous and endogenous metabolites to be visualized in 3D with subcellular resolution. We imaged the distribution of neurotransmitters-gamma-aminobutyric acid, dopamine and serotonin-with high spectroscopic confidence in the mouse hippocampus...
December 2017: Nature Methods
https://www.readbyqxmd.com/read/29130196/super-resolution-single-molecule-fish-at-the-drosophila-neuromuscular-junction
#11
Joshua S Titlow, Lu Yang, Richard M Parton, Ana Palanca, Ilan Davis
The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole mount Drosophila NMJ preparations to quantify primary transcription and count individual cytoplasmic mRNA molecules in specimens while maintaining ultrastructural preservation. The smFISH method is suitable for high-throughput sample processing and 3D image acquisition using any conventional microscopy imaging modality and is compatible with the use of antibody colabeling and transgenic fluorescent protein tags in axons, glia, synapses, and muscle cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29043668/preparation-and-imaging-of-specialized-er-using-super-resolution-and-tem-techniques
#12
Karen Bell, Karl Oparka, Kirsten Knox
The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM) which preserve the fragile structure and allow the detailed imaging of the SER...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29026706/development-of-novel-drug-eluting-stents-for-acute-myocardial-infarction
#13
REVIEW
Doo Sun Sim, Myung Ho Jeong
Delayed arterial healing at culprit sites after drug-eluting stent (DES) placement for acute myocardial infarction (AMI) is associated with increased risk of late stent thrombosis. The Korea Acute Myocardial Infarction Registry was established in commemoration of the 50(th) anniversary of Korea Circulation Society. Between November 2005 and December 2016, more than 62,000 patients were registered from 50 primary percutaneous coronary intervention (PCI) centers in Korea. DES in AMI may be safe and effective, however, there is concern about increased stent thrombosis after DES implantation in AMI patients, requiring longer-term dual anti-platelet therapy to reduce the risk of late stent thrombosis...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/28982361/distribution-of-plga-modified-nanoparticles-in-3d-cell-culture-models-of-hypo-vascularized-tumor-tissue
#14
Lee B Sims, Maya K Huss, Hermann B Frieboes, Jill M Steinbach-Rankins
BACKGROUND: Advanced stage cancer treatments are often invasive and painful-typically comprised of surgery, chemotherapy, and/or radiation treatment. Low transport efficiency during systemic chemotherapy may require high chemotherapeutic doses to effectively target cancerous tissue, resulting in systemic toxicity. Nanotherapeutic platforms have been proposed as an alternative to more safely and effectively deliver therapeutic agents directly to tumor sites. However, cellular internalization and tumor penetration are often diametrically opposed, with limited access to tumor regions distal from vasculature, due to irregular tissue morphologies...
October 5, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28976195/multimodal-super-resolution-optical-microscopy-using-a-transition-metal-based-probe-provides-unprecedented-capabilities-for-imaging-both-nuclear-chromatin-and-mitochondria
#15
Sreejesh Sreedharan, Martin R Gill, Esther Garcia, Hiwa K Saeed, Darren Robinson, Aisling Byrne, Ashley Cadby, Tia E Keyes, Carl Smythe, Patrina Pellett, Jorge Bernardino de la Serna, Jim A Thomas
Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable Ru(II) cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above ∼20 μM, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells...
October 26, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28966852/multifocus-structured-illumination-microscopy-for-fast-volumetric-super-resolution-imaging
#16
Sara Abrahamsson, Hans Blom, Ana Agostinho, Daniel C Jans, Aurelie Jost, Marcel Müller, Linnea Nilsson, Kristoffer Bernhem, Talley J Lambert, Rainer Heintzmann, Hjalmar Brismar
We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude...
September 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28964568/sound-wave-propagation-on-the-human-skull-surface-with-bone-conduction-stimulation
#17
Ivo Dobrev, Jae Hoon Sim, Stefan Stenfelt, Sebastian Ihrle, Rahel Gerig, Flurin Pfiffner, Albrecht Eiber, Alexander M Huber, Christof Röösli
BACKGROUND: Bone conduction (BC) is an alternative to air conduction to stimulate the inner ear. In general, the stimulation for BC occurs on a specific location directly on the skull bone or through the skin covering the skull bone. The stimulation propagates to the ipsilateral and contralateral cochlea, mainly via the skull bone and possibly via other skull contents. This study aims to investigate the wave propagation on the surface of the skull bone during BC stimulation at the forehead and at ipsilateral mastoid...
September 23, 2017: Hearing Research
https://www.readbyqxmd.com/read/28963440/superresolution-microscopy-of-the-%C3%AE-carboxysome-reveals-a-homogeneous-matrix
#18
Matthew J Niederhuber, Talley J Lambert, Clarence Yapp, Pamela A Silver, Jessica K Polka
Carbon fixation in cyanobacteria makes a major contribution to the global carbon cycle. The cyanobacterial carboxysome is a proteinaceous microcompartment that protects and concentrates the carbon-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in a paracrystalline lattice, making it possible for these organisms to fix CO2 from the atmosphere. The protein responsible for the organization of this lattice in beta-type carboxysomes of the freshwater cyanobacterium Synechococcus elongatus, CcmM, occurs in two isoforms thought to localize differentially within the carboxysome matrix...
October 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28906525/stereolithographic-printing-of-ionically-crosslinked-alginate-hydrogels-for-degradable-biomaterials-and-microfluidics
#19
Thomas M Valentin, Susan E Leggett, Po-Yen Chen, Jaskiranjeet K Sodhi, Lauren H Stephens, Hayley D McClintock, Jea Yun Sim, Ian Y Wong
3D printed biomaterials with spatial and temporal functionality could enable interfacial manipulation of fluid flows and motile cells. However, such dynamic biomaterials are challenging to implement since they must be responsive to multiple, biocompatible stimuli. Here, we show stereolithographic printing of hydrogels using noncovalent (ionic) crosslinking, which enables reversible patterning with controlled degradation. We demonstrate this approach using sodium alginate, photoacid generators and various combinations of divalent cation salts, which can be used to tune the hydrogel degradation kinetics, pattern fidelity, and mechanical properties...
October 11, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28883042/real-time-acquisition-of-transendothelial-electrical-resistance-in-an-all-human-in-vitro-3-dimensional-blood-brain-barrier-model-exemplifies-tight-junction-integrity
#20
Zaynah Maherally, Helen L Fillmore, Sim Ling Tan, Suk Fei Tan, Samah A Jassam, Friederike I Quack, Kathryn E Hatherell, Geoffrey J Pilkington
The blood-brain barrier (BBB) consists of endothelial cells, astrocytes, and pericytes embedded in basal lamina (BL). Most in vitro models use nonhuman, monolayer cultures for therapeutic-delivery studies, relying on transendothelial electrical resistance (TEER) measurements without other tight-junction (TJ) formation parameters. We aimed to develop reliable, reproducible, in vitro 3-dimensional (3D) models incorporating relevant human, in vivo cell types and BL proteins. The 3D BBB models were constructed with human brain endothelial cells, human astrocytes, and human brain pericytes in mono-, co-, and tricultures...
September 7, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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