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Jiachen Yang, Kyohoon Sim, Bin Jiang, Wen Lu
The no-reference (NR) quality assessment for stereoscopic images plays a significant role in 3D technology, but it also faces great challenges. In this paper, a novel NR stereo image quality assessment (SIQA) method is proposed. Based on the human visual system, this method mimics the summation and difference channels, which consider the binocular interactive perception property, to process the visual information. Especially, the summation and difference images are calculated by the contrast of hue and luminance in color patches...
May 10, 2018: Applied Optics
Lakshmi Balagopalan, Jason Yi, Tiffany Nguyen, Katherine M McIntire, Adam S Harned, Kedar Narayan, Lawrence E Samelson
The relative importance of plasma membrane-localized LAT versus vesicular LAT for microcluster formation and T-cell receptor (TCR) activation is unclear. Here, we show the sequence of events in LAT microcluster formation and vesicle delivery, using lattice light sheet microscopy to image a T cell from the earliest point of activation. A kinetic lag occurs between LAT microcluster formation and vesicular pool recruitment to the synapse. Correlative 3D light and electron microscopy show an absence of vesicles at microclusters at early times, but an abundance of vesicles as activation proceeds...
May 22, 2018: Nature Communications
Harma Ellens, Zhou Meng, Sylvain J Le Marchand, Joe Bentz
Introduction In vitro transporter kinetics are typically analyzed by steady-state Michaelis-Menten (MM) approximations. However, no clear evidence exists that these approximations, applied to multiple transporters in biological membranes, yield system-independent mechanistic parameters needed for reliable in vivo hypothesis generation and testing. Areas covered The classical mass action model has been developed for P-glycoprotein mediated transport across confluent polarized cell monolayers. Numerical integration of the mass action equations for transport using a stable global optimization program, yields fitted elementary rate constants that are system-independent...
May 23, 2018: Expert Opinion on Drug Metabolism & Toxicology
Van Son Nguyen, Van Huy Mai, Pascale Auban Senzier, Claude Pasquier, Kang Wang, Marcelo J Rozenberg, Nathalie Brun, Katia March, François Jomard, John Giapintzakis, Cristian N Mihailescu, Evripides Kyriakides, Pavan Nukala, Thomas Maroutian, Guillaume Agnus, Philippe Lecoeur, Silvia Matzen, Pascal Aubert, Sylvain Franger, Raphaël Salot, Pierre-Antoine Albouy, David Alamarguy, Brahim Dkhil, Pascal Chrétien, Olivier Schneegans
Lithium cobalt oxide nanobatteries offer exciting prospects in the field of nonvolatile memories and neuromorphic circuits. However, the precise underlying resistive switching (RS) mechanism remains a matter of debate in two-terminal cells. Herein, intriguing results, obtained by secondary ion mass spectroscopy (SIMS) 3D imaging, clearly demonstrate that the RS mechanism corresponds to lithium migration toward the outside of the Lix CoO2 layer. These observations are very well correlated with the observed insulator-to-metal transition of the oxide...
May 17, 2018: Small
Nurmohammed Patwary, Ana Doblas, Chrysanthe Preza
The performance of structured illumination microscopy (SIM) is hampered in many biological applications due to the inability to modulate the light when imaging deep into the sample. This is in part because sample-induced aberration reduces the modulation contrast of the structured pattern. In this paper, we present an image restoration approach suitable for processing raw incoherent-grid-projection SIM data with a low fringe contrast. Restoration results from simulated and experimental ApoTome SIM data show results with improved signal-to-noise ratio (SNR) and optical sectioning compared to the results obtained from existing methods, such as 2D demodulation and 3D SIM deconvolution...
April 1, 2018: Biomedical Optics Express
Ji-Young Sim, Yeon Jang, Woong-Chul Kim, Hae-Young Kim, Dong-Hwan Lee, Ji-Hwan Kim
PURPOSE: This study aimed to evaluate and compare the accuracy. METHODS: A reference model was prepared with three prepared teeth for three types of restorations: single crown, 3-unit bridge, and inlay. Stone models were fabricated from conventional impressions. Digital impressions of the reference model were created using an intraoral scanner (digital models). Physical models were fabricated using a three-dimensional (3D) printer. Reference, stone, and 3D printed models were subsequently scanned using an industrial optical scanner; files were exported in a stereolithography file format...
March 31, 2018: Journal of Prosthodontic Research
Shannon J L Pinnington, John F Marshall, Ann P Wheeler
Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively...
2018: Methods in Molecular Biology
Ivo Dobrev, Jae Hoon Sim
Recent measurements of vibrational motion to assess sound transmission through ear structures and skull contents have included three-dimensional (3D) behavior. The 3D motion of a point has been described with the three orthogonal components in the 3D space. In this article, a method to represent the 3D velocity with the magnitude and phase of the resultant velocity is introduced. This method was applied to the measurement of cochlear promontory motion as an indication of bone conduction (BC) sound transmission...
March 21, 2018: Hearing Research
Benjamin B A Raymond, Ranya Madhkoor, Ina Schleicher, Cord C Uphoff, Lynne Turnbull, Cynthia B Whitchurch, Manfred Rohde, Matthew P Padula, Steven P Djordjevic
Mycoplasma hyopneumoniae , an agriculturally important porcine pathogen, disrupts the mucociliary escalator causing ciliostasis, loss of cilial function, and epithelial cell death within the porcine lung. Losses to swine production due to growth rate retardation and reduced feed conversion efficiency are severe, and antibiotics are used heavily to control mycoplasmal pneumonia. Notably, little is known about the repertoire of host receptors that M. hyopneumoniae targets to facilitate colonization. Here we show, for the first time, that actin exists extracellularly on porcine epithelial monolayers (PK-15) using surface biotinylation and 3D-Structured Illumination Microscopy (3D-SIM), and that M...
2018: Frontiers in Cellular and Infection Microbiology
Marike Rüder, Benedikt M Nagel, Sven Bogdan
The most abundant immune cells in Drosophila are macrophage-like plasmatocytes that fulfill central roles in morphogenesis, immune and tissue damage response. The various genetic tools available in Drosophila together with high-resolution and live-imaging microscopy techniques make Drosophila macrophages an excellent model system that combines many advantages of cultured cells with in vivo genetics. Here, we describe the isolation and staining of macrophages from larvae for ex vivo structured illumination microscopy (SIM), the preparation of white prepupae for in vivo 2D random cell migration analysis, and the preparation of pupae (18 h after puparium formation, APF) for in vivo 3D directed cell migration analysis upon wounding using spinning disk microscopy...
2018: Methods in Molecular Biology
Xin-Xin Jin, Xiao-Xiang Chen, Jing Xiang, Yun-Zhou Chen, Li-Hui Jia, Bing-Wu Wang, Shun-Cheung Cheng, Xin Zhou, Chi-Fai Leung, Song Gao
A series of homoleptic mononuclear 8-coordinate FeII and CoII compounds, [FeII (L2 )2 ](ClO4 )2 (2), [FeII (L3 )2 ](ClO4 )2 (3), [FeII (L4 )2 ](ClO4 )2 (4), [CoII (L1 )2 ](ClO4 )2 (5), [CoII (L2 )2 ](ClO4 )2 (6), [CoII (L3 )2 ](ClO4 )2 (7), and [CoII (L4 )2 ](ClO4 )2 (8) (L1 and L2 are 2,9-dialkylcarboxylate-1,10-phenanthroline ligands; L3 and L4 are 6,6'-dialkylcarboxylate-2,2'-bipyridine ligands), have been obtained, and their crystal structures have been determined by X-ray crystallography. The metal center in all of these compounds has an oversaturated coordination number of 8, which is completed by two neutral homoleptic tetradentate ligands and is unconventional in 3d-metal compounds...
April 2, 2018: Inorganic Chemistry
Kazuaki Sawada, Ryosuke Kawakami, Ryuichi Shigemoto, Tomomi Nemoto
Three-dimensional (3D) super-resolution microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along the dendrite has not been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the excitation laser induced by light scattering and optical aberrations. To address this issue and solve these optical problems, we applied a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration depth and the spatial resolution were improved in LUCID-treated slices, and 160-nm spatial resolution was obtained in a large portion of the imaging volume on a single apical dendrite...
May 2018: European Journal of Neuroscience
Anja Henss, Svenja-K Otto, Kaija Schaepe, Linda Pauksch, Katrin S Lips, Marcus Rohnke
Within this study, the authors use human mesenchymal stem cells incubated with silver nanoparticles (AgNPs) as a model system to systematically investigate the advantages and drawbacks of the fast imaging delayed extraction mode for two-dimensional and three-dimensional (3D) analyses at the cellular level. The authors compare the delayed extraction mode with commonly employed measurement modes in terms of mass and lateral resolution, intensity, and dose density. Using the delayed extraction mode for single cell analysis, a high mass resolution up to 4000 at m/z = 184...
February 28, 2018: Biointerphases
Qinglin Deng, Mengjiao Li, Junyong Wang, Kai Jiang, Zhigao Hu, Junhao Chu
Orthorhombic Nb2 O5 (T-Nb2 O5 ) has structural merit but poor electrical conductivity, limiting their applications in energy storage. Although graphene is frequently adopted to effectively improve its electrochemical properties, the ordinary modified methods cannot meet the growing demands for high-performance. Here, we demonstrate that different graphene modified routes play a vital role in affecting the electrochemical performances of T-Nb2 O5 . By only manual shaking within one minute, Nb2 O5 nano-particles can be rapidly adsorbed onto graphene, then the free-anchored T-Nb2 O5 @graphene three-dimensional networks can be successfully prepared based on hydrogel method...
May 4, 2018: Nanotechnology
Keunhee Kim, Hee-Seung Choi, Nan-Sim Pang
This report presents two cases of tooth autotransplantation using cone-beam computed tomography (CBCT), the three-dimensional (3D) simulation dental planning software and a computer-aided rapid prototyping (CARP) model. Two hopeless teeth of adult patients were replaced as their third molar teeth. Before deciding the autotransplantation, diagnostic CBCT images were acquired and imported to SimPlant software. The SimPlant dental program was used for surgical simulation prior to autotransplantation, which created 3D images of the available donor teeth and recipient site tooth and superimposed the images to display their morphological similarity...
February 16, 2018: Australian Endodontic Journal: the Journal of the Australian Society of Endodontology Inc
Thibault Lagache, Alexandre Grassart, Stéphane Dallongeville, Orestis Faklaris, Nathalie Sauvonnet, Alexandre Dufour, Lydia Danglot, Jean-Christophe Olivo-Marin
Elucidating protein functions and molecular organisation requires to localise precisely single or aggregated molecules and analyse their spatial distributions. We develop a statistical method SODA (Statistical Object Distance Analysis) that uses either micro- or nanoscopy to significantly improve on standard co-localisation techniques. Our method considers cellular geometry and densities of molecules to provide statistical maps of isolated and associated (coupled) molecules. We use SODA with three-colour structured-illumination microscopy (SIM) images of hippocampal neurons, and statistically characterise spatial organisation of thousands of synapses...
February 15, 2018: Nature Communications
Young Chul Sim, Seung-Hyuk Lim, Yang-Seok Yoo, Min-Ho Jang, Sunghan Choi, Hwan-Seop Yeo, Kie Young Woo, Sangwon Lee, Hyun Gyu Song, Yong-Hoon Cho
Warm and natural white light (i.e., with a correlated colour temperature <5000 K) with good colour rendition (i.e., a colour rendering index >75) is in demand as an indoor lighting source of comfortable interior lighting and mood lighting. However, for warm white light, phosphor-converted white light-emitting diodes (WLEDs) require a red phosphor instead of a commercial yellow phosphor (YAG:Ce3+ ), and suffer from limitations such as unavoidable energy conversion losses, degraded phosphors and high manufacturing costs...
March 8, 2018: Nanoscale
Min Feng, Ming-Liang Tong
Single-ion magnets (SIMs), exhibiting slow magnetization relaxation in the absence of the magnetic field, originate from their single spin-carrier centre. In pursuit of high-performance magnetic properties, such as high spin-reversal barrier and high blocking temperature, various metal centres were investigated to establish SIMs, including 3d and 5d transition metal ions, 4f lanthanide ions, and 5f actinide ions, which possess unique zero-field splitting and magnetic properties. Therefore, proper ligand field is of great importance to different types of metals...
January 31, 2018: Chemistry: a European Journal
Firoj Ali, Sunil Aute, Sreejesh Sreedharan, H A Anila, Hiwa K Saeed, Carl G Smythe, Jim A Thomas, Amitava Das
BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.
February 15, 2018: Chemical Communications: Chem Comm
J-H Kwon, H-T Kim, S J Sim, Y J Cha, J Lee
The objective of this study is to develop an accurate, rapid, simple, and label-free assay technology that enables point-of-care diagnosis of AIDS. For this, 3-dimensional (3D) probes to sensitively detect anti-HIV antibodies were designed and synthesized by genetically presenting a HIV antigen (gp41) on the surface of engineered human ferritin nanoparticles. The 3D probes also present multi-copies of the hexa-histidine peptide (H6 ) on their surface to chemisorb gold ions (Au3+ ), which is essential for the generation and self-enhancement of assay signals...
February 12, 2018: Analyst
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