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HPV PCR cytology

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https://www.readbyqxmd.com/read/28541628/high-risk-human-papillomavirus-genotype-distribution-and-attribution-to-cervical-cancer-and-precancerous-lesions-in-a-rural-chinese-population
#1
Xue Lian Zhao, Shang Ying Hu, Qian Zhang, Li Dong, Rui Mei Feng, Ross Han, Fang Hui Zhao
OBJECTIVE: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening tests and the development of new polyvalent HPV vaccines among the Chinese population. METHODS: One thousand two hundred ninety-two subjects were followed based on the Shanxi Province Cervical Cancer Screening Study I (SPOCCS-I), and screened by HPV DNA testing (hybrid capture® 2 [HC2]), liquid-based cytology (LBC), and if necessary, directed or random colposcopy-guided quadrant biopsies...
July 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28511636/hpv-prevalence-and-type-distribution-in-cypriot-women-with-cervical-cytological-abnormalities
#2
George Krashias, Dana Koptides, Christina Christodoulou
BACKGROUND: Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19-65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated...
May 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28431571/unique-lcr-variations-among-lineages-of-hpv16-18-and-45-isolates-from-women-with-normal-cervical-cytology-in-ghana
#3
Adolf K Awua, Richard M K Adanu, Edwin K Wiredu, Edwin A Afari, Vanessa A Zubuch, Richard H Asmah, Alberto Severini
BACKGROUND: In addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28426837/the-high-prevalence-of-hpv-and-hpv16-european-variants-in-cervical-and-anal-samples-of-hiv-seropositive-women-with-normal-pap-test-results
#4
Lays Paula Bondi Volpini, Neide Aparecida Tosato Boldrini, Luciana Bueno de Freitas, Angelica Espinosa Miranda, Liliana Cruz Spano
Human Immunodeficiency Virus (HIV)-seropositive women are more likely to have anogenital cancer, and high risk-HPV (HR-HPV) infection is the main associated factor. Between August 2013 and December 2015, we conducted a descriptive study to determine the HPV genotypes and HPV16 variants in cervical and anal samples of HIV-seropositive women with a normal Pap test. The viral DNA was amplified by PCR using the PGMY09/11 set of primers. Reverse line blot (RLB), restriction fragment length polymorphism (RFLP) and sequencing assays were used to determine the HPV genotypes...
2017: PloS One
https://www.readbyqxmd.com/read/28390433/evaluation-of-the-performance-of-human-papillomavirus-testing-in-paired-urine-and-clinician-collected-cervical-samples-among-women-aged-over-30%C3%A2-years-in-bhutan
#5
Ugyen Tshomo, Silvia Franceschi, Tshokey Tshokey, Tashi Tobgay, Iacopo Baussano, Vanessa Tenet, Peter J F Snijders, Tarik Gheit, Massimo Tommasino, Alex Vorsters, Gary M Clifford
BACKGROUND: Urine sampling may offer a less invasive solution than cervical sampling to test for human papillomavirus (HPV) for HPV vaccine impact monitoring. METHODS: Paired samples of urine and exfoliated cervical cells were obtained for 89 women with history of high-risk (HR) HPV-positive normal cytology in Bhutan. Urine sampling protocol included self-collection of first-void urine immediately into a conservation medium and procedures to optimize DNA yield. Colposcopical abnormalities were biopsied...
April 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28377417/cervical-infection-with-cutaneous-beta-and-mucosal-alpha-papillomaviruses
#6
Laura Sichero, Mariam El-Zein, Emily M Nunes, Silvaneide Ferreira, Eduardo L Franco, Luisa L Villa
BACKGROUND: Alpha-human papillomavirus (α-HPV) play a causal role in cervical cancer but little is known about the epidemiology of genital Beta-HPV infection. METHODS: We used Luminex and polymerase chain reaction (PCR) hybridization to detect β- and α-HPVs prevalence at enrolment and 12-months follow-up in cervical samples from 505 women enrolled in the Ludwig-McGill cohort study. We compared epidemiological correlates of both β- and α-HPVs and compared genotypes between these genera with respect to co-occurrence and association with cervical cytological abnormalities...
April 4, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28294358/prevalence-of-human-papillomavirus-infection-among-chilean-women-from-2012-to-2016
#7
Nicolás Vergara, Gloria Espinoza, Monserrat Balanda, Andrea Quiero, Wilma Hidalgo, Héctor San Martín, Alejandro Ramírez, Eugenio Ramírez
Here, we evaluated the prevalence of Human Papillomavirus (HPV) in two groups of Chilean women. The first group consisted of 3235 women aged 18-64 years attended in six primary care centers of Santiago. The second group consisted of 456 women 18-85 aged who consulted the Gynaecology Department of the Reference Hospital of Santiago. Samples were collected from October 2012 to February 2016. Cervical swabs were analyzed both HPV genotyping by PCR and Reverse Line Blot, and cervical cytology by Pap testing. Results showed a prevalence of 12...
March 11, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28288568/deep-sequencing-of-hpv-e6-e7-genes-reveals-loss-of-genotypic-diversity-and-gain-of-clonal-dominance-in-high-grade-intraepithelial-lesions-of-the-cervix
#8
Jane Shen-Gunther, Yufeng Wang, Zhao Lai, Graham M Poage, Luis Perez, Tim H M Huang
BACKGROUND: Human papillomavirus (HPV) is the carcinogen of almost all invasive cervical cancer and a major cause of oral and other anogenital malignancies. HPV genotyping by dideoxy (Sanger) sequencing is currently the reference method of choice for clinical diagnostics. However, for samples with multiple HPV infections, genotype identification is singular and occasionally imprecise or indeterminable due to overlapping chromatograms. Our aim was to explore and compare HPV metagenomes in abnormal cervical cytology by deep sequencing for correlation with disease states...
March 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28285422/current-and-future-techniques-for-human-papilloma-virus-hpv-testing-in-oropharyngeal-squamous-cell-carcinoma
#9
REVIEW
Ali Qureishi, Thomas Mawby, Lisa Fraser, Ketan A Shah, Henrik Møller, Stuart Winter
Despite a reduction in smoking and alcohol consumption, the incidence of oropharyngeal squamous cell carcinoma (OPSCC) is rising. This is attributed to human papilloma virus (HPV) infection and screening for HPV is now recommended in all cases of OPSCC. Despite a variety of clinically available tests and new non-invasive test strategies there is no consensus on which technique is best. This review reports on current techniques for HPV detection in OPSCC and the clinical applicability of emerging techniques...
March 11, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28120391/cervical-cancer-incidence-after-screening-with-hpv-cytology-and-visual-methods-18-year-follow-up-of-the-guanacaste-cohort
#10
Ana Cecilia Rodríguez, Carlos Ávila, Rolando Herrero, Allan Hildesheim, Mark E Sherman, Robert D Burk, Jorge Morales, Mario Alfaro, Diego Guillén, María Ethel Trejos, Rosa María Vargas, Guillermo Torres, Mark Schiffman
Testing negative for human papillomavirus (HPV) predicts long-term reassurance against invasive cervical cancer (ICC). To provide realistic estimates of effectiveness for new screening programs, we studied ICC risk after a 7-year repeated multimethod screening effort. In 1993-1994, 10,049 women aged 18-97 years were enrolled into a population-based cohort study of cervical HPV in Guanacaste, Costa Rica. Women were screened at different intervals according to enrollment results. Each visit (mean 3.2, 90% attendance) included split-sample conventional, automated, and liquid-based cytology, visual inspection, cervicography, and PCR-based HPV testing...
April 15, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28115985/human-papillomavirus-infection-in-anal-intraepithelial-lesions-from-hiv-infected-cuban-men
#11
Celia M Limia, Yudira Soto, Yanara García, Orestes Blanco, Vivian Kourí, María V López, María E Toledo, Lissette Pérez, Yoanna Baños, Yaniris Caturla, Francisco Aguayo
BACKGROUND: An association between HPV infection and progression to anal squamous intraepithelial lesions (ASIL) has been established, specifically in high-risk populations such as HIV-infected men. In this population, anal cancer is one of the most common non-AIDS-defining malignancies. METHODS: A cross-sectional study to detect anal lesions and HPV infection was performed. Anal mucosa samples were collected from 56 HIV-infected men from Cuba. The cytological diagnosis was done according to Bethesda 2001 System...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28069334/prevalence-of-anal-infection-due-to-high-risk-human-papillomavirus-and-analysis-of-e2-gene-integrity-among-women-with-cervical-abnormalities
#12
María Del Refugio Gonzalez-Losa, Marylin Puerto-Solís, Guadalupe Ayora-Talavera, Jesús Gómez-Carvallo, Alejandra Euán-López, José I Cisneros-Cutz, Ariel Rosado-López, Jesúa Echeverría Salazar, Laura Conde-Ferráez
BACKGROUND: High-risk human papillomaviruses (HR-HPV) infection has been associated with 90% of anal cancer cases. Women with abnormal cytology are a high-risk group to develop anal neoplasia. The aim of this study is to describe the prevalence and epidemiology of HR-HPV 16, 18, 45, and 58 anal infections in women with cervical abnormalities, as well as to assess E2 gene integrity. METHODS: A cross-sectional study was performed on 311 cervical and 311 anal samples from patients with abnormal cytology in two colposcopy clinics in Yucatan, Mexico...
January 6, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28056826/hpv-genotypes-distribution-in-indian-women-with-and-without-cervical-carcinoma-implication-for-hpv-vaccination-program-in-odisha-eastern-india
#13
Rashmirani Senapati, Bhagyalaxmi Nayak, Shantanu Kumar Kar, Bhagirathi Dwibedi
BACKGROUND: Considering the limited cross protection offered by the current HPV vaccines, understanding the HPV genotype distribution among the different population is essential in predicting the efficacy of current vaccine and devising new vaccine strategy. The present work aimed at investigating the HPV genotypes distribution among women with and without cervical carcinoma in Odisha, Eastern India. METHODS: A total of 607 participants have been enrolled between January 2014 and June 2016...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28046025/human-papillomavirus-hpv-infection-molecular-epidemiology-genotyping-seroprevalence-and-associated-risk-factors-among-arab-women-in-qatar
#14
Asha A Elmi, Devendra Bansal, Anushree Acharya, Sini Skariah, Soha R Dargham, Laith J Abu-Raddad, Nady Mohamed-Nady, Paul Amuna, Asma A J Al-Thani, Ali A Sultan
Human Papillomavirus (HPV) infections are known to cause cervical cancer worldwide, however, limited information is currently available on prevalence, types distribution and risk factors for HPV infection in the Arab countries. We conducted a cross-sectional observational study exclusively of women of Arabic origin residing in Qatar (n = 406) who were selected from the Women's Hospital at Hamad Medical Corporation (HMC) and Health Centers of the Primary Health Care Corporation in Doha, Qatar over the period March 2013 to August 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28028296/human-papillomavirus-hpv-in-egyptian-females-study-by-cytology-histopathology-colposcopy-and-molecular-diagnosis-of-high-risk-types
#15
M A Nayel, O G Shaker, A N Hosni, H N Hosni, S E Khalifa, A F Shazly
OBJECTIVES: In Northern Africa, the region Egypt belongs to, about 10.7% of women are estimated to harbour cervical human papillomavirus (HPV) infection and 78.4% of invasive cancers are attributed to HPVs 16 or 18. We aimed at comparing HPV detection by ISH-PCR tissue with other conventional available cheaper techniques, finding which of them can be relied upon in a developing country like Egypt for HPV detection. METHODS: Sixty patients were included. For them colposcopy, PAP smear, histopathology and detection of HPV using ISH PCR tissue and PCR swab were achieved...
December 2016: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/27927273/cervical-infection-of-oncogenic-human-papillomavirus-hpv-types-in-beijing-china
#16
Ying Li, Ke Huang, Pei Li Ji, Lei Song, Hong Tu Liu
OBJECTIVE: This study was designed to determine the prevalence of oncogenic human papillomavirus (HPV) in cervical infections in Beijing, China, and to investigate the odds ratio (OR) of HPV single and multiple infections in abnormal cytology. METHODS: A total of 19,018 specimens from outpatients in the department of obstetric and gynecology were collected. They were detected using high-risk HPV genotyping real-time polymerase chain reaction (PCR) kit and analyzed by ThinPrep cytology test for cervical pathological diagnosis...
October 2016: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/27898186/human-papillomavirus-dna-detection-in-fine-needle-aspirates-as-indicator-of-human-papillomavirus-positive-oropharyngeal-squamous-cell-carcinoma-a-prospective-study
#17
Lars Sivars, David Landin, Linnea Haeggblom, Nikolaos Tertipis, Nathalie Grün, Cinzia Bersani, Linda Marklund, Mehran Ghaderi, Anders Näsman, Torbjörn Ramqvist, Cecilia Nordfors, Eva Munck-Wikland, Edneia Tani, Tina Dalianis
BACKGROUND: Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (SCC) has a better outcome than most head neck squamous cell carcinomas (HNSCCs) and an HPV-positive lymph node metastasis likely has an HPV-positive oropharyngeal SCC origin. Determining HPV-status in cervical lymph nodes by fine-needle aspiration cytology (FNAC) may be useful for diagnosis. METHODS: FNACs from 66 patients with neck masses were prospectively examined for HPV DNA and HPV16 mRNA by a polymerase chain reaction (PCR)-based assay, and the data correlated to diagnosis and HPV-status obtained from histopathological specimens...
March 2017: Head & Neck
https://www.readbyqxmd.com/read/27896251/combined-sybr-green-real-time-polymerase-chain-reaction-and-microarray-method-for-the-simultaneous-determination-of-human-papillomavirus-loads-and-genotypes
#18
Hyun Hee Seo, Young Jun Kim, Mi Seon Jeong, Sung Ran Hong, In Ho Lee, Kyeong A So, Mi-Kyung Kim, Yoo Kyung Lee, Ki Heon Lee, Juree Kim, Sung Jae Kim, Tae Jin Kim
OBJECTIVE: The aim of this study was to describe the principle of the Cheil HPV DNA Chip assay and evaluate its accuracy. In order to quantify the human papillomavirus (HPV) load and identify HPV genotypes simultaneously, this assay combined the two methods: SYBR Green quantitative real-time polymerase chain reaction (PCR) and DNA microarray. METHODS: We designed novel consensus primer sets that target the conserved region of the HPV L1 gene for quantifying and detecting a broad range of HPV types by quantitative real-time PCR...
November 2016: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/27894986/application-of-molecular-genotyping-to-determine-prevalence-of-hpv-strains-in-pap-smears-of-kazakhstan-women
#19
Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova, Zaurech Abugalieva, Korlukain Seksembayeva, Syed Ali, Azliyati Azizan
OBJECTIVES: Human papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. METHODS: Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic...
January 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27892681/evaluating-the-existence-of-small-compressed-binucleated-squamous-cells-in-asc-h
#20
Mitsuaki Okodo, Kaori Okayama, Hiroshi Kitamura, Natsuko Shiina, Timothy Caniz, Midori Ono, Hiromi Yabusaki
Purpose: To evaluate the legitimacy of a diagnosis of ASC-H in 5 cases which were followed up monthly for over 2 years with both cytology and HPV testing. Methods: Some 5 cases out of a total of 25.0 self-sampled Pap test patients diagnosed as ASC-H provided 119 specimens over 2 years, with HPV-DNA testing perormed using a E6 primer. Results: Cases 1, 2 and 3 showed SIL after the ASC-H diagnosis, while cases 4 and 5 showed and maintained NILM. Cases 1, 2 and 3 were further characterized by small atypical compressed binucleated cells, in which HPV was detected by in situ PCR...
October 1, 2016: Asian Pacific Journal of Cancer Prevention: APJCP
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