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HPV PCR cytology

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https://www.readbyqxmd.com/read/29138009/human-papillomavirus-hpv-infection-in-females-with-normal-cervical-cytology-genotyping-and-phylogenetic-analysis-among-women-in-punjab-pakistan
#1
Hafsa Aziz, Huma Iqbal, Humera Mahmood, Shazia Fatima, Muhammad Faheem, Areej Abdul Sattar, Sobia Tabassum, Sanum Napper, Syeda Batool, Nuzhat Rasheed
OBJECTIVE: Cervical cancer is the fourth most common cancer in the world, seventh overall with account for an estimated 300,000 annual deaths. HPV is the second most common cause of cervical cancer worldwide. HPV screening is not a common practice in Pakistan. The present study aims to determine the prevalence of HPV and HPV types in females with normal cytology of cervix in Upper and Lower region of Punjab Pakistan and analyze the risk factors for HPV in this region. METHOD: In this study, we performed PCR analysis for 1011 female patients with normal cytology of cervix from various districts in Punjab Province, Pakistan and risk factors for acquisition of HPV were studied...
November 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29100063/spectrum-of-hpv-types-before-and-after-treatment-of-cervical-intraepithelial-neoplasia-grade-2-and-3
#2
Ylva Lindroth, Kaj Bjelkenkrantz, Ola Forslund
BACKGROUND: To monitor residual disease after treatment of high grade cervical intraepithelial neoplasia (CIN), cytology together with human papillomavirus (HPV) testing are commonly performed. OBJECTIVES: To analyse the spectrum of HPV types before and after treatment. STUDY DESIGN: This register-based study included 446 women treated for CIN2 or 3, where cytology samples had been HPV-tested before and after treatment by the use of the MGP-PCR Luminex HPV L1-DNA-assay identifying 39 HPV types, including 12 high risk (HR) HPV types...
October 31, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29093458/prevalence-and-genotype-distribution-of-human-papillomavirus-in-961-029-screening-tests-in-southeastern-china-zhejiang-province-between-2011-and-2015
#3
Xiao Chen, Haiou Xu, Wanwan Xu, Wenjie Zeng, Jinwei Liu, Qing Wu, Xiaofeng Zhao, Tang Jiang
Human papillomavirus infection plays a key role in the development of cervical cancer. To establish a foundation for HPV-based screening and vaccination programs, we investigated the HPV prevalence and genotypic distributions in Chinese women from Zhejiang Province. Between 2011 and 2015, a total of 961,029 samples from 2021 clinical hospitals were tested HPV genotype by a PCR-based hybridization gene chip assay, and 443,890 samples were evaluated cervical cytology by liquid-based cytology analysis. Our results showed that the positive rate for HPV was 20...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29090037/performance-of-a-methylation-specific-real-time-pcr-assay-as-a-triage-test-for-hpv-positive-women
#4
Martina Schmitz, Kristina Wunsch, Heike Hoyer, Cornelia Scheungraber, Ingo B Runnebaum, Alfred Hansel, Matthias Dürst
BACKGROUND: HPV DNA testing as a primary screening marker is being implemented in several countries. Due to the high HPV prevalence in the screening population, effective triage strategies for HPV-positive cases are required. The aim of this study was to evaluate the performance of a methylation-specific real-time PCR  assay (GynTect®) comprising six marker regions as a triage test. RESULTS: An analytical sensitivity of 0.1 ng genomic DNA corresponding to 15 SiHa cells was achieved...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28981551/predictors-of-hpv-incidence-and-clearance-in-a-cohort-of-brazilian-hiv-infected-women
#5
Ana Gabriela Travassos, Eduardo Netto, Eveline Xavier-Souza, Isabella Nóbrega, Karina Adami, Maiara Timbó, Karen Abbehusen, Sheyla Fernandes, Camila Duran, Tatiana Haguihara, Fábio Ferreira, Carlos Brites
Persistent infection with high-risk human papillomavirus (HR-HPV) is necessary for the development of precursor lesions and cervical cancer. HPV infection among women living with HIV/AIDS (WLHA) occurs more frequently, presents a higher rate of persistent infections and an earlier progression to cancer. We aimed to evaluate HR-HPV prevalence, incidence and clearance, and its association with HIV viral suppression, immunological response and other risk factors among WLHA followed at an STD/HIV reference center...
2017: PloS One
https://www.readbyqxmd.com/read/28977944/utility-of-gene-methylation-analysis-cytological-examination-and-hpv-16-18-genotyping-in-triage-of-high-risk-human-papilloma-virus-positive-women
#6
Yan Tian, Na-Yi Yuan Wu, Yu-Ligh Liou, Ching-Tung Yeh, Lanqin Cao, Ya-Nan Kang, Huei-Jen Wang, Yichen Li, Tang-Yuan Chu, Wei Li, Xiang Liu, Yi Zhang, Honghao Zhou, Yu Zhang
In 2015, the American Society for Colposcopy and Cervical Pathology and the Society of Gynecologic Oncology issued interim guidance for the use of a human papillomavirus (HPV) test for primary screening, suggesting triage of women positive for high-risk human papillomavirus (hrHPV) by HPV-16/18 genotyping and cytology for women positive for non-16/18 hrHPV. The design of the present study was based on this interim guidance and analysis of the methylation status of specific candidate genes, which has been proposed as a tool to reduce unnecessary referral following primary HPV screening for cervical cancer...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28953633/risk-factors-for-infection-by-oncogenic-human-papillomaviruses-in-hiv-positive-msm-patients-in-the-art-era-2010-2016
#7
Carmen Hidalgo-Tenorio, Concepción Gil-Anguita, Jessica Ramírez-Taboada, Javier Esquivias, Miguel A López-Ruz, Omar Mohamed Balgahata, Rosario Javier-Martinez, Juan Pasquau
Squamous cell carcinoma of anus (SCCA) is one of the most frequent non-AIDS-defining diseases in HIV patients, mainly in men who have sex with men (MSM), and it is associated with human papillomavirus (HPV) infection.To determine the prevalence of high-risk HPV (HR-HPV) genotypes, premalignant lesions (HSIL) and SCCA in a cohort of HIV-positive MSM; to study the distribution of HPV genotypes according to anal histology results; and to analyze risk factors for this infection.This prospective single-center study was conducted between May 2010 and September 2016...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28952287/significance-of-compression-in-binucleation-while-differentiating-reactive-cellular-changes-between-human-papillomavirus-and-candida-infections
#8
Mitsuaki Okodo, Kaori Okayama, Tadasi Fukui, Natsuko Shiina, Timothy Caniz, Hiromi Yabusaki, Masahiko Fujii
Purpose: Binucleation is a reactive cellular change (RCC) in Pap smears due to Candida infection. However, the origin of these binucleated cells as RCCs remains unclear. The purpose of this study was to examine binucleation in patients negative for intraepithelial lesion or malignancy (NILM) and infected with Candida and those infected with high-risk human papillomavirus (hr-HPV) and to clarify the origin of the binucleated cells. Methods: A total of 115 endocervical swab specimens with a combined diagnosis of NILM, Candida infection, and RCCs were used for this study...
September 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28919602/comparative-study-of-cytology-and-histopathology-of-cervical-lesion-in-via-positive-patients-and-its-correlation-with-high-risk-human-papilloma-virus
#9
N P Ansari, F M Saleh, S M Shahida, E N Rahman
Cervical cancer is the second most frequent type of cancer and leading cause of mortality among women worldwide. The present study was undertaken to assess precancerous and cancerous cervical lesion by cytology as well as HPV DNA identification and their comparison with histopathology in VIA positive cases. This descriptive, cross-sectional type of observational study was carried out in the Department of Pathology, Mymensingh Medical College in collaboration with the Department of Gynecology and Obstetrics, Mymensingh Medical College Hospital and Department of Microbiology and Hygiene in Bangladesh Agriculture University for HPV DNA detection from July 2012 to June 2013...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28918430/distribution-of-chlamydia-trachomatis-genotypes-in-infective-diseases-of-the-female-lower-genital-tract
#10
Yujie Chen, Jing Chen, Lan Yang, Yanming Jiang, Li Li, Wenjuan Yi, Lifang Lan, Liuhong Zhang
The purpose of this study was to investigate the distribution of Chlamydia trachomatis (CT) genotypes in infective diseases of the female lower genital tract, especially in cervical diseases. This study included 128 CT-positive women. DNA was extracted from cervical swabs. Omp1 gene PCR-RFLP and sequencing were used to confirm the subtypes of CT. The association of subtypes with age, clinical symptoms, cervical cytology, and biopsy results was further analyzed. Omp1 gene PCR-RFLP and sequencing showed that the order of prevalent CT genotypes in the female lower genital tract was D (n=38, 29...
September 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28913693/human-papillomavirus-genotype-distribution-in-cervical-samples-among-vaccine-na%C3%A3-ve-barbados-women
#11
Juann M Ward, Kolin Schmalenberg, Nick A Antonishyn, Ian R Hambleton, Elizabeth L Blackman, Paul N Levett, Marquita V Gittens-St Hilaire
PURPOSE: This study was undertaken to provide baseline HPV genotype distribution among women in Barbados before HPV immunization was introduced. This information would then be used as a denominator for post-vaccine surveillance and is expected to aid in understanding the effect of vaccination on cervical disease in Barbados. METHODS: Liquid-based cytology specimens were collected from 413 women (age range 18-65 years) attending three clinics, in a pre-vaccination, population-based study...
November 2017: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/28852043/comparison-of-vaginal-microbiota-sampling-techniques-cytobrush-versus-swab
#12
Anita Mitra, David A MacIntyre, Vishakha Mahajan, Yun S Lee, Ann Smith, Julian R Marchesi, Deirdre Lyons, Phillip R Bennett, Maria Kyrgiou
Evidence suggests the vaginal microbiota (VM) may influence risk of persistent Human Papillomavirus (HPV) infection and cervical carcinogenesis. Established cytology biobanks, typically collected with a cytobrush, constitute a unique resource to study such associations longitudinally. It is plausible that compared to rayon swabs; the most commonly used sampling devices, cytobrushes may disrupt biofilms leading to variation in VM composition. Cervico-vaginal samples were collected with cytobrush and rayon swabs from 30 women with high-grade cervical precancer...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28840524/prevalence-of-anal-human-papillomavirus-infection-in-hungarian-men-who-have-sex-with-men
#13
Eszter Szabó, Csaba Kósa, Edit Babarczi, Mihály Sulyok, Eszter Ujhelyi, Dénes Bánhegyi, István Vályi-Nagy
Anal cancer is one of the leading causes of death in non-AIDS defining cancers. Most of these cancers are associated with high risk HPV infection. So far, the prevalence and the significance of anal HPV infection have not been studied in the Hungarian MSM population. The main objective of our study was to determine the prevalence and associated risk factors of HPV-infection in the Hungarian MSM community, particularly in HIV-infected MSM. Out of 109 examinations 92 samples (80 HIV-infected and 12 HIV-negative MSM) were evaluated for both cytological abnormalities and HPV genotyping PCR...
August 24, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28789619/high-prevalence-of-co-infection-between-human-papillomavirus-hpv-51-and-52-in-mexican-population
#14
Jazbet Gallegos-Bolaños, Jessica Alejandra Rivera-Domínguez, José Miguel Presno-Bernal, Rodolfo Daniel Cervantes-Villagrana
BACKGROUND: Human papillomavirus (HPV) is associated with the genesis of cervical carcinoma. The co-infection among HPV genotypes is frequent, but the clinical significance is controversial; in Mexico, the prevalence and pattern of co-infection differ depending on the geographic area of study. We analyzed the mono- and co-infection prevalence of multiple HPV genotypes, as well as preferential interactions among them in a Mexico City sample population. METHODS: This study was designed as a retrospective cohort study...
August 8, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28748500/digital-droplet-pcr-ddpcr-for-the-detection-and-quantification-of-hpv-16-18-33-and-45%C3%A2-a-short-report
#15
Gabriella Lillsunde Larsson, Gisela Helenius
PURPOSE: Human papilloma virus (HPV) infection is associated with several anogenital malignancies. Here, we set out to evaluate digital droplet PCR (ddPCR) as a tool for HPV 16, 18, 33 and 45 viral load quantification and, in addition, to compare the efficacy of the ddPCR assay for HPV 16 detection with that of quantitative real-time PCR (qPCR). METHODS: Clinical samples, positive for HPV genotypes 16, 18, 33 and 45 were analyzed for viral load using ddPCR. Sample DNA was cleaved before droplet generation and PCR...
October 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28739302/comparison-of-the-analytical-and-clinical-performance-of-five-tests-for-the-detection-of-human-papillomavirus-genital-infection
#16
M Del Pino, I Alonso, A Rodriguez-Trujillo, S Bernal, D Geraets, N Guimerà, A Torne, J Ordi
HPV-based screening provides greater protection against cervical cancer (CC) than cytology-based strategies. Currently, several molecular diagnostic assays for the detection of human papillomavirus (HPV) are available. In this study, we analyzed 5 different HPV testing and genotyping techniques (Hybrid Capture 2 [HC2; Qiagen, Hilden, Germany], AnyplexTMII HPV28 [Anyplex; Seegene, Seoul, Korea], Linear Array [Roche, Branchburg, NJ, USA], GP5+/6+ PCR-EIA-RH [Labo Bio-medical Products, Rijswijk, The Netherlands] and CLART2 [Genomica, Madrid, Spain]) in 295 women referred to the hospital Colposcopy Clinic from 2007 to 2008 due to positive HPV test results or an abnormal Pap test...
October 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28729879/safety-and-immunogenicity-of-the-quadrivalent-human-papillomavirus-qhpv-vaccine-in-hiv-positive-spanish-men-who-have-sex-with-men-msm
#17
Carmen Hidalgo-Tenorio, Jessica Ramírez-Taboada, Concepción Gil-Anguita, Javier Esquivias, Mohamed Omar-Mohamed-Balgahata, Antonio SamPedro, Miguel Lopez-Ruz, Juan Pasquau
BACKGROUND: Safety and immunogenicity of the quadrivalent human papillomavirus (qHPV) vaccine were evaluated in HIV-positive Spanish MSM. The prevalence of High Squamous Intraepithelial Lesions (HSIL) and genotypes of high-risk human papillomavirus (HR-HPV) were also determined, as well as risk factors associated with the presence of HR-HPV in anal mucosa. METHODS: This is a randomised, double blind, placebo-controlled trial of the quadrivalent HPV (qHPV) vaccine...
2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28723690/distribution-of-human-papillomavirus-genotypes-among-women-in-mashhad-iran
#18
Eskandar Taghizadeh, Forough Taheri, Hamed Abdolkarimi, Pedram Ghorbani Renani, Seyed Mohammad Gheibi Hayat
OBJECTIVE: Cervical cancer is the third most common malignancy in females. Since the human papillomavirus (HPV) genotype can vary geographically, it is essential to define its genotypic distribution before establishing health care policies and vaccination programs in any given area. The aim of this study was to determine the frequency of HPV types in women in Mashhad, Iran. METHODS: We used nested PCR and reverse dot-blot hybridization for genotyping. The study included 143 cervical cytology samples from Mashhad with confirmed papillomavirus infection by molecular methods...
2017: Intervirology
https://www.readbyqxmd.com/read/28720147/safety-and-immunogenicity-of-the-quadrivalent-human-papillomavirus-qhpv-vaccine-in-hiv-positive-spanish-men-who-have-sex-with-men-msm
#19
Carmen Hidalgo-Tenorio, Jessica Ramírez-Taboada, Concepción Gil-Anguita, Javier Esquivias, Mohamed Omar-Mohamed-Balgahata, Antonio SamPedro, Miguel Lopez-Ruz, Juan Pasquau
BACKGROUND: Safety and immunogenicity of the quadrivalent human papillomavirus (qHPV) vaccine were evaluated in HIV-positive Spanish MSM. The prevalence of High Squamous Intraepithelial Lesions (HSIL) and genotypes of high-risk human papillomavirus (HR-HPV) were also determined, as well as risk factors associated with the presence of HR-HPV in anal mucosa. METHODS: This is a randomised, double blind, placebo-controlled trial of the quadrivalent HPV (qHPV) vaccine...
July 18, 2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28700010/argon-plasma-versus-electrofulguration-in-the-treatment-of-anal-and-perianal-condylomata-acuminata-in-patients-with-acquired-immunodeficiency-virus
#20
Eduardo Angelo Braga, Gaspar de Jesus Lopes, Sarhan Sydney Saad
Purpose: To compare the effectiveness of anal and perianal condylomata treatment using argon plasma and electrofulguration. Methods: From January 2013 to April 2014, 37 patients with anal and perianal condylomata, who had been diagnosed through proctological examination, oncotic cytology, polymerase chain reaction (PCR) and histology, underwent treatment with argon plasma and electrofulguration. The perianal and anal regions were divided into two semicircles. Each semicircle was treated using one of the methods by means of simple randomization...
June 2017: Acta Cirúrgica Brasileira
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