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HPV PCR cytology

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https://www.readbyqxmd.com/read/29341505/stability-integrity-and-recovery-rate-of-cellular-nucleic-acids-preserved-in-a-new-liquid-based-cytology-medium
#1
Guodong Zhao, Zhaocheng Liu, Yaping Tian, Minsheng Zhu, Shouli Wang, Hui Wang, Shen Gao, Yong Ma, Minxue Zheng
BACKGROUND: Liquid-based cytology (LBC) has replaced the conventional Papanicolaou test in cervical cancer screening. The cervical swab specimens collected in LBC media can also be used for additional analyses including high-risk HPV (HR-HPV) test, DNA methylation analysis, and HPV E6/E7 mRNA test. METHODS: The stability, integrity, and recovery rate of cellular DNA and RNA after storage at different conditions were evaluated by a quantitative real-time PCR (qPCR) based HR-HPV test, reverse transcription qPCR (RT-qPCR), and agarose gel electrophoresis...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29334204/detection-of-chlamydia-trachomatis-in-pap-smear-samples-from-south-khorasan-province-of-iran
#2
Davod Javanmard, Mahmoodreza Behravan, Malaknaz Ghannadkafi, Alireza Salehabadi, Masood Ziaee, Mohammad Hasan Namaei
Background: Chlamydia trachomatis (CT), the most common bacterial sexually transmitted infection (STI), leads to pelvic inflammatory disease, infertility and chronic pelvic pain in women as well as an increased risk of vertical transmission, conjunctivitis and pneumonitis in infants. It may also be a co-factor along with human papillomavirus (HPV) in cervical cancer progression. We aimed to determine the prevalence of CT genotypes in genital specimens of women from South Khorasan, Iran and to test the association between CT and cytology statistics...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29328473/detection-of-high-grade-neoplasia-in-air-dried-cervical-pap-smears-by-a-microrna-based-classifier
#3
Mikhail K Ivanov, Sergei E Titov, Sergei A Glushkov, Victoria V Dzyubenko, Anastasia V Malek, Polina A Arkhangelskaya, Roman B Samsonov, Andrey A Mikhetko, Elena V Bakhidze, Igor V Berlev, Nikolay N Kolesnikov
Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed...
January 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29302583/tp53-r72p-polymorphism-and-susceptibility-to-human-papillomavirus-infection-among-women-with-human-immunodeficiency-virus-in-morocco-a-case-control-study
#4
Ahd Oulad Lahsen, Hanâ Baba, Rajaa Bensghir, Naouar Fayssel, Mustapha Sodqi, Latifa Marih, Sellama Nadifi, Lahcen Wakrim, Kamal Marhoum El Filali, Sayeh Ezzikouri
Background: Human papillomavirus (HPV) is the most common sexually transmitted agent worldwide. HPV is the main causative agent for cervical cancer. The HPV oncoprotein E6 binds to the tumor suppressor gene product p53, promoting its degradation; the Arg allele of TP53 R72P polymorphism binds more ardently with HPV E6 than the Pro variant. Here, we investigated whether TP53 R72P gene variant, rs104252, was associated with susceptibility to HPV infection in women with human immunodeficiency virus (HIV)...
December 2017: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29279588/comparison-of-the-sensitivity-and-specificity-of-p16-ki-67-dual-staining-and-hpv-dna-testing-of-abnormal-cervical-cytology-in-the-detection-of-histology-proven-cervical-intraepithelial-neoplasia-grade-2-and-above-cin-2
#5
T K Y Tay, K L Lim, M H Hilmy, A A Thike, S T Goh, L H Song, J S G Hwang, S Mantoo
INTRODUCTION: Human papillomavirus (HPV) testing is used as a means of triaging cervico-vaginal smears with low grade squamous abnormalities or as part of co-testing with cytology. While HPV testing has a high sensitivity, it has a low specificity in detecting cervical intraepithelial neoplasia grade 2 and above (CIN 2+) leading to unnecessary colposcopy referrals. We investigate the accuracy of the p16/Ki-67 dual immunocytochemical stain in determining the presence of CIN 2+ lesions on histology and its potential as a superior biomarker for triage...
December 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/29273004/impact-of-hpv-vaccination-with-gardasil%C3%A2-in-switzerland
#6
Martine Jacot-Guillarmod, Jérôme Pasquier, Gilbert Greub, Massimo Bongiovanni, Chahin Achtari, Roland Sahli
BACKGROUND: Gardasil®, a quadrivalent vaccine targeting low-risk (6, 11) and high-risk (16, 18) human papillomaviruses (HPV), has been offered to 11-14 year-old schoolgirls in Switzerland since 2008. To evaluate its success and its potential impact on cervical cancer screening, HPV genotypes were examined in 18-year-old girls five years later (sub-study 1) and in outpatients participating to cervical cancer screening before and after vaccine implementation (sub-study 2). METHODS: For sub-study 1, 3726 females aged 18 in 2013 were invited to fill a questionnaire on personal demographics and HPV risk factors and to provide a self-collected cervicovaginal sample for HPV genotyping and Chlamydia trachomatis PCR...
December 22, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29238853/detection-and-genotyping-of-hpv-in-urine-samples-from-chilean-women-attending-primary-health-care-centers
#7
Nicolás Vergara, Monserrat Balanda, Wilma Hidalgo, Héctor San Martín, Alexis Aceituno, Francisco Roldán, Tania Villalón, Melissa Hott, Gloria Espinoza, Andrea Quiero, María T Valenzuela, Eugenio Ramírez
Cervical cancer is the second most common malignant neoplasm in women worldwide representing approximately 10% of all types of cancers. Triage of women through cervical cytology has been an important strategy for the surveillance and control of new cases of cervical cancer. However, in many regions around the world cervical cytology has a low coverage compared to developed countries. The molecular detection of HPV is the most effective method to increase the screening sensitivity of women at risk of developing cervical cancer...
December 14, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29208455/high-prevalence-and-incidence-of-hpv-related-anal-cancer-precursor-lesions-in-hiv-positive-women-in-the-late-haart-era
#8
Carmen Hidalgo-Tenorio, Samantha E de Jesus, Javier Esquivias, Juan Pasquau
INTRODUCTION: Anal cancer is one of the most common non-AIDS defining malignancies, especially in men who have sex with men and women living with HIV (WLHIV). OBJECTIVES: To evaluate the prevalence and incidence of precursor lesions (high-grade squamous intraepithelial lesions [HSIL]) and anal cancer in our cohort of women and to compare them to cervical lesions; to calculate the percentage of patients that acquire and clear oncogenic genotypes (HR-HPV) in the anal canal; and to determine predictive factors for anal HPV infection...
December 2, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29182899/five-year-experience-of-the-treatment-of-squamous-cell-carcinoma-of-the-anus
#9
C A Leo, C Santorelli, J D Hodgkinson, O Bidovaneta, F Baldelli, F Cantarella, E Cavazzoni
OBJECTIVES: The best treatment of early stage anal squamous cell carcinoma (SCC) is under debated. Wide local excision (WLE) may be considered adequate for stage 1 anal margin cancer. This study demonstrates our experience in treatment of patients with SCC over 5 years. PATIENTS AND METHODS: We conducted a retrospective study of patients who had undergone anal screening or anal cancer surveillance between October 2010 and 2015 in our department. Each patient underwent anal Pap test, HPV test PCR HPV DNA and cytology by Thin Prep...
July 2017: Il Giornale di Chirurgia
https://www.readbyqxmd.com/read/29182627/human-papillomavirus-dna-detection-in-plasma-and-cervical-samples-of-women-with-a-recent-history-of-low-grade-or-precancerous-cervical-dysplasia
#10
Clementina Elvezia Cocuzza, Marianna Martinelli, Federica Sina, Andrea Piana, Giovanni Sotgiu, Tiziana Dell'Anna, Rosario Musumeci
Circulating HPV DNA has been previously described in women with advanced stages of cervical cancer and has been suggested to be a prognostic marker of disease recurrences and metastases. Only a few studies have reported the presence of HPV DNA in bloodstream of patients with low grade or precancerous cervical lesions. This study aimed to define if HPV DNA could be detected in plasma samples of 120 women referred for a recent history of cervical dysplasia who presented with lesions ranging from High Squamous Intraepithelial Lesion (H-SIL) to regressed normal cytology...
2017: PloS One
https://www.readbyqxmd.com/read/29166976/clinical-laboratory-and-epidemiological-aspects-of-hpv-infection-in-a-low-income-population-from-south-bahia-brazil
#11
S R Gadelha, D M V Soares-Barreto, G B Costa, V C N Leal, L G S Gomes, U R Santos, G C S Ferreira, L D Carvalho, S M V Soraes-Almeida, M A G Mello, A P M Mariano, S M B Sousa, A R Vago, L J Marin
The aim of this study was to determine the prevalence and risk factors for human papillomavirus (HPV) infection in the Southern region of the State of Bahia, evaluating the performance of alternative complementary methods for cervical lesion detection. Cervical samples from women who attended healthcare units were collected and diagnosed by visual inspection, cervical cytology and nested polymerase chain reaction (PCR). Moreover, hemi-nested PCR was performed to detect different HPV genotypes. The prevalence of HPV infection was 47·7%, with genotype 16 detected in most cases...
November 23, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29161285/hpv-genotypes-co-infections-associated-with-cervical-carcinoma-special-focus-on-phylogenetically-related-and-non-vaccine-targeted-genotypes
#12
Rashmirani Senapati, Bhagyalaxmi Nayak, Shantanu Kumar Kar, Bhagirathi Dwibedi
HPV is the major causative agent for cervical cancer. Study on the risk of cervical cancer associated with different hr-HPV genotypes would be useful for disease management and new vaccine strategy. With limited reports available, the present study aimed to investigate the pattern of HPV genotypes coinfections and risk of cervical carcinoma associated with them in Indian population. 15 HPV genotypes were detected by E6/E7 multiplex nested type-specific PCR in the HPV-positive cervical samples of 172 cervical cancer cases and 174 subjects with normal cytology...
2017: PloS One
https://www.readbyqxmd.com/read/29138009/human-papillomavirus-hpv-infection-in-females-with-normal-cervical-cytology-genotyping-and-phylogenetic-analysis-among-women-in-punjab-pakistan
#13
Hafsa Aziz, Huma Iqbal, Humera Mahmood, Shazia Fatima, Muhammad Faheem, Areej Abdul Sattar, Sobia Tabassum, Sanum Napper, Syeda Batool, Nuzhat Rasheed
OBJECTIVE: Cervical cancer is the fourth most common cancer in the world, seventh overall with account for an estimated 300,000 annual deaths. HPV is the second most common cause of cervical cancer worldwide. HPV screening is not a common practice in Pakistan. The present study aims to determine the prevalence of HPV and HPV types in females with normal cytology of cervix in Upper and Lower region of Punjab Pakistan and analyze the risk factors for HPV in this region. METHOD: In this study, we performed PCR analysis for 1011 female patients with normal cytology of cervix from various districts in Punjab Province, Pakistan and risk factors for acquisition of HPV were studied...
November 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29100063/spectrum-of-hpv-types-before-and-after-treatment-of-cervical-intraepithelial-neoplasia-grade-2-and-3
#14
Ylva Lindroth, Kaj Bjelkenkrantz, Ola Forslund
BACKGROUND: To monitor residual disease after treatment of high grade cervical intraepithelial neoplasia (CIN), cytology together with human papillomavirus (HPV) testing are commonly performed. OBJECTIVES: To analyse the spectrum of HPV types before and after treatment. STUDY DESIGN: This register-based study included 446 women treated for CIN2 or 3, where cytology samples had been HPV-tested before and after treatment by the use of the MGP-PCR Luminex HPV L1-DNA-assay identifying 39 HPV types, including 12 high risk (HR) HPV types...
December 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29093458/prevalence-and-genotype-distribution-of-human-papillomavirus-in-961-029-screening-tests-in-southeastern-china-zhejiang-province-between-2011-and-2015
#15
Xiao Chen, Haiou Xu, Wanwan Xu, Wenjie Zeng, Jinwei Liu, Qing Wu, Xiaofeng Zhao, Tang Jiang
Human papillomavirus infection plays a key role in the development of cervical cancer. To establish a foundation for HPV-based screening and vaccination programs, we investigated the HPV prevalence and genotypic distributions in Chinese women from Zhejiang Province. Between 2011 and 2015, a total of 961,029 samples from 2021 clinical hospitals were tested HPV genotype by a PCR-based hybridization gene chip assay, and 443,890 samples were evaluated cervical cytology by liquid-based cytology analysis. Our results showed that the positive rate for HPV was 20...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29090037/performance-of-a-methylation-specific-real-time-pcr-assay-as-a-triage-test-for-hpv-positive-women
#16
Martina Schmitz, Kristina Wunsch, Heike Hoyer, Cornelia Scheungraber, Ingo B Runnebaum, Alfred Hansel, Matthias Dürst
BACKGROUND: HPV DNA testing as a primary screening marker is being implemented in several countries. Due to the high HPV prevalence in the screening population, effective triage strategies for HPV-positive cases are required. The aim of this study was to evaluate the performance of a methylation-specific real-time PCR  assay (GynTect®) comprising six marker regions as a triage test. RESULTS: An analytical sensitivity of 0.1 ng genomic DNA corresponding to 15 SiHa cells was achieved...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28981551/predictors-of-hpv-incidence-and-clearance-in-a-cohort-of-brazilian-hiv-infected-women
#17
Ana Gabriela Travassos, Eduardo Netto, Eveline Xavier-Souza, Isabella Nóbrega, Karina Adami, Maiara Timbó, Karen Abbehusen, Sheyla Fernandes, Camila Duran, Tatiana Haguihara, Fábio Ferreira, Carlos Brites
Persistent infection with high-risk human papillomavirus (HR-HPV) is necessary for the development of precursor lesions and cervical cancer. HPV infection among women living with HIV/AIDS (WLHA) occurs more frequently, presents a higher rate of persistent infections and an earlier progression to cancer. We aimed to evaluate HR-HPV prevalence, incidence and clearance, and its association with HIV viral suppression, immunological response and other risk factors among WLHA followed at an STD/HIV reference center...
2017: PloS One
https://www.readbyqxmd.com/read/28977944/utility-of-gene-methylation-analysis-cytological-examination-and-hpv-16-18-genotyping-in-triage-of-high-risk-human-papilloma-virus-positive-women
#18
Yan Tian, Na-Yi Yuan Wu, Yu-Ligh Liou, Ching-Tung Yeh, Lanqin Cao, Ya-Nan Kang, Huei-Jen Wang, Yichen Li, Tang-Yuan Chu, Wei Li, Xiang Liu, Yi Zhang, Honghao Zhou, Yu Zhang
In 2015, the American Society for Colposcopy and Cervical Pathology and the Society of Gynecologic Oncology issued interim guidance for the use of a human papillomavirus (HPV) test for primary screening, suggesting triage of women positive for high-risk human papillomavirus (hrHPV) by HPV-16/18 genotyping and cytology for women positive for non-16/18 hrHPV. The design of the present study was based on this interim guidance and analysis of the methylation status of specific candidate genes, which has been proposed as a tool to reduce unnecessary referral following primary HPV screening for cervical cancer...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28953633/risk-factors-for-infection-by-oncogenic-human-papillomaviruses-in-hiv-positive-msm-patients-in-the-art-era-2010-2016
#19
Carmen Hidalgo-Tenorio, Concepción Gil-Anguita, Jessica Ramírez-Taboada, Javier Esquivias, Miguel A López-Ruz, Omar Mohamed Balgahata, Rosario Javier-Martinez, Juan Pasquau
Squamous cell carcinoma of anus (SCCA) is one of the most frequent non-AIDS-defining diseases in HIV patients, mainly in men who have sex with men (MSM), and it is associated with human papillomavirus (HPV) infection.To determine the prevalence of high-risk HPV (HR-HPV) genotypes, premalignant lesions (HSIL) and SCCA in a cohort of HIV-positive MSM; to study the distribution of HPV genotypes according to anal histology results; and to analyze risk factors for this infection.This prospective single-center study was conducted between May 2010 and September 2016...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28952287/significance-of-compression-in-binucleation-while-differentiating-reactive-cellular-changes-between-human-papillomavirus-and-candida-infections
#20
Mitsuaki Okodo, Kaori Okayama, Tadasi Fukui, Natsuko Shiina, Timothy Caniz, Hiromi Yabusaki, Masahiko Fujii
Purpose: Binucleation is a reactive cellular change (RCC) in Pap smears due to Candida infection. However, the origin of these binucleated cells as RCCs remains unclear. The purpose of this study was to examine binucleation in patients negative for intraepithelial lesion or malignancy (NILM) and infected with Candida and those infected with high-risk human papillomavirus (hr-HPV) and to clarify the origin of the binucleated cells. Methods: A total of 115 endocervical swab specimens with a combined diagnosis of NILM, Candida infection, and RCCs were used for this study...
September 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
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