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HPV PCR cytology

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https://www.readbyqxmd.com/read/28919602/comparative-study-of-cytology-and-histopathology-of-cervical-lesion-in-via-positive-patients-and-its-correlation-with-high-risk-human-papilloma-virus
#1
N P Ansari, F M Saleh, S M Shahida, E N Rahman
Cervical cancer is the second most frequent type of cancer and leading cause of mortality among women worldwide. The present study was undertaken to assess precancerous and cancerous cervical lesion by cytology as well as HPV DNA identification and their comparison with histopathology in VIA positive cases. This descriptive, cross-sectional type of observational study was carried out in the Department of Pathology, Mymensingh Medical College in collaboration with the Department of Gynecology and Obstetrics, Mymensingh Medical College Hospital and Department of Microbiology and Hygiene in Bangladesh Agriculture University for HPV DNA detection from July 2012 to June 2013...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28918430/distribution-of-chlamydia-trachomatis-genotypes-in-infective-diseases-of-the-female-lower-genital-tract
#2
Yujie Chen, Jing Chen, Lan Yang, Yanming Jiang, Li Li, Wenjuan Yi, Lifang Lan, Liuhong Zhang
The purpose of this study was to investigate the distribution of Chlamydia trachomatis (CT) genotypes in infective diseases of the female lower genital tract, especially in cervical diseases. This study included 128 CT-positive women. DNA was extracted from cervical swabs. Omp1 gene PCR-RFLP and sequencing were used to confirm the subtypes of CT. The association of subtypes with age, clinical symptoms, cervical cytology, and biopsy results was further analyzed. Omp1 gene PCR-RFLP and sequencing showed that the order of prevalent CT genotypes in the female lower genital tract was D (n=38, 29...
September 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28913693/human-papillomavirus-genotype-distribution-in-cervical-samples-among-vaccine-na%C3%A3-ve-barbados-women
#3
Juann M Ward, Kolin Schmalenberg, Nick A Antonishyn, Ian R Hambleton, Elizabeth L Blackman, Paul N Levett, Marquita V Gittens-St Hilaire
PURPOSE: This study was undertaken to provide baseline HPV genotype distribution among women in Barbados before HPV immunization was introduced. This information would then be used as a denominator for post-vaccine surveillance and is expected to aid in understanding the effect of vaccination on cervical disease in Barbados. METHODS: Liquid-based cytology specimens were collected from 413 women (age range 18-65 years) attending three clinics, in a pre-vaccination, population-based study...
September 14, 2017: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/28852043/comparison-of-vaginal-microbiota-sampling-techniques-cytobrush-versus-swab
#4
Anita Mitra, David A MacIntyre, Vishakha Mahajan, Yun S Lee, Ann Smith, Julian R Marchesi, Deirdre Lyons, Phillip R Bennett, Maria Kyrgiou
Evidence suggests the vaginal microbiota (VM) may influence risk of persistent Human Papillomavirus (HPV) infection and cervical carcinogenesis. Established cytology biobanks, typically collected with a cytobrush, constitute a unique resource to study such associations longitudinally. It is plausible that compared to rayon swabs; the most commonly used sampling devices, cytobrushes may disrupt biofilms leading to variation in VM composition. Cervico-vaginal samples were collected with cytobrush and rayon swabs from 30 women with high-grade cervical precancer...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28840524/prevalence-of-anal-human-papillomavirus-infection-in-hungarian-men-who-have-sex-with-men
#5
Eszter Szabó, Csaba Kósa, Edit Babarczi, Mihály Sulyok, Eszter Ujhelyi, Dénes Bánhegyi, István Vályi-Nagy
Anal cancer is one of the leading causes of death in non-AIDS defining cancers. Most of these cancers are associated with high risk HPV infection. So far, the prevalence and the significance of anal HPV infection have not been studied in the Hungarian MSM population. The main objective of our study was to determine the prevalence and associated risk factors of HPV-infection in the Hungarian MSM community, particularly in HIV-infected MSM. Out of 109 examinations 92 samples (80 HIV-infected and 12 HIV-negative MSM) were evaluated for both cytological abnormalities and HPV genotyping PCR...
August 24, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28789619/high-prevalence-of-co-infection-between-human-papillomavirus-hpv-51-and-52-in-mexican-population
#6
Jazbet Gallegos-Bolaños, Jessica Alejandra Rivera-Domínguez, José Miguel Presno-Bernal, Rodolfo Daniel Cervantes-Villagrana
BACKGROUND: Human papillomavirus (HPV) is associated with the genesis of cervical carcinoma. The co-infection among HPV genotypes is frequent, but the clinical significance is controversial; in Mexico, the prevalence and pattern of co-infection differ depending on the geographic area of study. We analyzed the mono- and co-infection prevalence of multiple HPV genotypes, as well as preferential interactions among them in a Mexico City sample population. METHODS: This study was designed as a retrospective cohort study...
August 8, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28748500/digital-droplet-pcr-ddpcr-for-the-detection-and-quantification-of-hpv-16-18-33-and-45%C3%A2-a-short-report
#7
Gabriella Lillsunde Larsson, Gisela Helenius
PURPOSE: Human papilloma virus (HPV) infection is associated with several anogenital malignancies. Here, we set out to evaluate digital droplet PCR (ddPCR) as a tool for HPV 16, 18, 33 and 45 viral load quantification and, in addition, to compare the efficacy of the ddPCR assay for HPV 16 detection with that of quantitative real-time PCR (qPCR). METHODS: Clinical samples, positive for HPV genotypes 16, 18, 33 and 45 were analyzed for viral load using ddPCR. Sample DNA was cleaved before droplet generation and PCR...
July 26, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28739302/comparison-of-the-analytical-and-clinical-performance-of-five-tests-for-the-detection-of-human-papillomavirus-genital-infection
#8
M Del Pino, I Alonso, A Rodriguez-Trujillo, S Bernal, D Geraets, N Guimerà, A Torne, J Ordi
HPV-based screening provides greater protection against cervical cancer (CC) than cytology-based strategies. Currently, several molecular diagnostic assays for the detection of human papillomavirus (HPV) are available. In this study, we analyzed 5 different HPV testing and genotyping techniques (Hybrid Capture 2 [HC2; Qiagen, Hilden, Germany], AnyplexTMII HPV28 [Anyplex; Seegene, Seoul, Korea], Linear Array [Roche, Branchburg, NJ, USA], GP5+/6+ PCR-EIA-RH [Labo Bio-medical Products, Rijswijk, The Netherlands] and CLART2 [Genomica, Madrid, Spain]) in 295 women referred to the hospital Colposcopy Clinic from 2007 to 2008 due to positive HPV test results or an abnormal Pap test...
October 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28729879/safety-and-immunogenicity-of-the-quadrivalent-human-papillomavirus-qhpv-vaccine-in-hiv-positive-spanish-men-who-have-sex-with-men-msm
#9
Carmen Hidalgo-Tenorio, Jessica Ramírez-Taboada, Concepción Gil-Anguita, Javier Esquivias, Mohamed Omar-Mohamed-Balgahata, Antonio SamPedro, Miguel Lopez-Ruz, Juan Pasquau
BACKGROUND: Safety and immunogenicity of the quadrivalent human papillomavirus (qHPV) vaccine were evaluated in HIV-positive Spanish MSM. The prevalence of High Squamous Intraepithelial Lesions (HSIL) and genotypes of high-risk human papillomavirus (HR-HPV) were also determined, as well as risk factors associated with the presence of HR-HPV in anal mucosa. METHODS: This is a randomised, double blind, placebo-controlled trial of the quadrivalent HPV (qHPV) vaccine...
2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28723690/distribution-of-human-papillomavirus-genotypes-among-women-in-mashhad-iran
#10
Eskandar Taghizadeh, Forough Taheri, Hamed Abdolkarimi, Pedram Ghorbani Renani, Seyed Mohammad Gheibi Hayat
OBJECTIVE: Cervical cancer is the third most common malignancy in females. Since the human papillomavirus (HPV) genotype can vary geographically, it is essential to define its genotypic distribution before establishing health care policies and vaccination programs in any given area. The aim of this study was to determine the frequency of HPV types in women in Mashhad, Iran. METHODS: We used nested PCR and reverse dot-blot hybridization for genotyping. The study included 143 cervical cytology samples from Mashhad with confirmed papillomavirus infection by molecular methods...
July 20, 2017: Intervirology
https://www.readbyqxmd.com/read/28720147/safety-and-immunogenicity-of-the-quadrivalent-human-papillomavirus-qhpv-vaccine-in-hiv-positive-spanish-men-who-have-sex-with-men-msm
#11
Carmen Hidalgo-Tenorio, Jessica Ramírez-Taboada, Concepción Gil-Anguita, Javier Esquivias, Mohamed Omar-Mohamed-Balgahata, Antonio SamPedro, Miguel Lopez-Ruz, Juan Pasquau
BACKGROUND: Safety and immunogenicity of the quadrivalent human papillomavirus (qHPV) vaccine were evaluated in HIV-positive Spanish MSM. The prevalence of High Squamous Intraepithelial Lesions (HSIL) and genotypes of high-risk human papillomavirus (HR-HPV) were also determined, as well as risk factors associated with the presence of HR-HPV in anal mucosa. METHODS: This is a randomised, double blind, placebo-controlled trial of the quadrivalent HPV (qHPV) vaccine...
July 18, 2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28700010/argon-plasma-versus-electrofulguration-in-the-treatment-of-anal-and-perianal-condylomata-acuminata-in-patients-with-acquired-immunodeficiency-virus
#12
Eduardo Angelo Braga, Gaspar de Jesus Lopes, Sarhan Sydney Saad
Purpose: To compare the effectiveness of anal and perianal condylomata treatment using argon plasma and electrofulguration. Methods: From January 2013 to April 2014, 37 patients with anal and perianal condylomata, who had been diagnosed through proctological examination, oncotic cytology, polymerase chain reaction (PCR) and histology, underwent treatment with argon plasma and electrofulguration. The perianal and anal regions were divided into two semicircles. Each semicircle was treated using one of the methods by means of simple randomization...
June 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28692681/cervical-hpv-type-specific-pre-vaccination-prevalence-and-age-distribution-in-croatia
#13
Ivan Sabol, Nina Milutin Gašperov, Mihaela Matovina, Ksenija Božinović, Goran Grubišić, Ivan Fistonić, Dragan Belci, Laia Alemany, Sonja Džebro, Mara Dominis, Mario Šekerija, Sara Tous, Silvia de Sanjosé, Magdalena Grce
The main etiological factor of precancerous lesion and invasive cervical cancer are oncogenic human papillomaviruses types (HPVs). The objective of this study was to establish the distribution of the most common HPVs in different cervical lesions and cancer prior to the implementation of organized population-based cervical screening and HPV vaccination in Croatia. In this study, 4,432 cervical specimens, collected through a 16-year period, were tested for the presence of HPV-DNA by polymerase chain reaction (PCR) with three sets of broad-spectrum primers and type-specific primers for most common low-risk (LR) types (HPV-6, 11) and the most common high-risk (HR) types (HPV-16, 18, 31, 33, 45, 52, 58)...
2017: PloS One
https://www.readbyqxmd.com/read/28658700/prevalence-of-human-papillomavirus-infection-and-cervical-cancer-screening-among-riverside-women-of-the-brazilian-amazon
#14
Daniel Valim Duarte, Rodrigo Covre Vieira, Elza Baía de Brito, Maria da Conceição Nascimento Pinheiro, Jeniffer do Socorro Valente Monteiro, Mário Diego Rocha Valente, Edna Aoba Yassui Ishikawa, Hellen Thais Fuzii, Maísa Silva de Sousa
Purpose The aim of this study was to evaluate the overall and type-specific prevalence of human papillomavirus (HPV) infection among females living in riverside communities in the state of Pará, in the Eastern Brazilian Amazon. These communities are inhabited by low-income people, and are accessible only by small boats. Cervical cytology and risk factors for HPV infection were also assessed. Methods Cervical samples from 353 women of selected communities were collected both for Papanicolau (Pap) test and HPV detection...
July 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28657218/clinical-validation-of-the-pcr-reverse-dot-blot-human-papillomavirus-genotyping-test-in-cervical-lesions-from-chinese-women-in-the-fujian-province-a-hospital-based-population-study
#15
Pengming Sun, Yiyi Song, Guanyu Ruan, Xiaodan Mao, Yafang Kang, Binhua Dong, Fen Lin
OBJECTIVE: To determine the clinical significance of the polymerase chain reaction (PCR)-reverse dot blot (RDB) human papillomavirus (HPV) genotyping assay in cervical cancer screening. METHODS: A total of 10,442 women attending the Fujian Provincial Maternity and Children's Health Hospital were evaluated using the liquid-based cytology (thinprep cytologic test [TCT]) and the PCR-RDB HPV test. Women with HPV infection and/or abnormal cytology were referred for colposcopy and biopsy...
September 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28643492/human-papillomavirus-genotype-distribution-among-18-815-women-in-13-korean-cities-and-relationship-with-cervical-cytology-findings
#16
Eun Hee Nah, Seon Cho, Suyoung Kim, Han Ik Cho
BACKGROUND: The prevalence and genotype distribution of Human papillomavirus (HPV) infection vary depending on geographical region and the immunity provided by vaccines. This study aimed to clarify the recent prevalence and genotype distribution of HPV according to age and cervical cytology findings in Korea. METHODS: This study included 18,815 health examinees that underwent cervical cytology and HPV genotyping tests at 16 centers of Korean Association of Health Promotion in 13 cities in Korea, between January 2014 and October 2015...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28608497/the-effect-of-hpv-infection-on-ivf-outcomes-a-systematic-review-and-meta-analysis
#17
REVIEW
Charalampos Siristatidis, Dennis Vaidakis, Eleni Sertedaki, Wellington Martins
OBJECTIVES: Tο identify, appraise and summarize the available data, concerning the impact of HPV infection on the reproductive outcomes following in vitro fertilization (IVF). METHODS: We searched for studies on PubMed, Embase, Scopus, Lilacs and the Cochrane Central Register of Controlled Trials from inception to Mar-2017. Any type of HPV infection in the woman assessed through PCR, subfertility factors and IVF indications and protocols were considered. Our primary outcomes were live birth/ongoing pregnancy and miscarriage, while secondary included clinical and laboratory parameters...
June 13, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28541628/high-risk-human-papillomavirus-genotype-distribution-and-attribution-to-cervical-cancer-and-precancerous-lesions-in-a-rural-chinese-population
#18
Xue Lian Zhao, Shang Ying Hu, Qian Zhang, Li Dong, Rui Mei Feng, Ross Han, Fang Hui Zhao
OBJECTIVE: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening tests and the development of new polyvalent HPV vaccines among the Chinese population. METHODS: One thousand two hundred ninety-two subjects were followed based on the Shanxi Province Cervical Cancer Screening Study I (SPOCCS-I), and screened by HPV DNA testing (hybrid capture® 2 [HC2]), liquid-based cytology (LBC), and if necessary, directed or random colposcopy-guided quadrant biopsies...
July 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28511636/hpv-prevalence-and-type-distribution-in-cypriot-women-with-cervical-cytological-abnormalities
#19
George Krashias, Dana Koptides, Christina Christodoulou
BACKGROUND: Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19-65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated...
May 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28431571/unique-lcr-variations-among-lineages-of-hpv16-18-and-45-isolates-from-women-with-normal-cervical-cytology-in-ghana
#20
Adolf K Awua, Richard M K Adanu, Edwin K Wiredu, Edwin A Afari, Vanessa A Zubuch, Richard H Asmah, Alberto Severini
BACKGROUND: In addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies...
April 21, 2017: Virology Journal
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