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HPV PCR cytology

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https://www.readbyqxmd.com/read/29764400/low-cost-hpv-testing-and-the-prevalence-of-cervical-infection-in-asymptomatic-populations-in-guatemala
#1
Hong Lou, Eduardo Gharzouzi, Sarita Polo Guerra, Joël Fokom Domgue, Julie Sawitzke, Guillermo Villagran, Lisa Garland, Joseph F Boland, Sarah Wagner, Héctor Rosas, Jami Troxler, Heidi McMillen, Bailey Kessing, Enrique Alvirez, Miriam Castillo, Hesler Morales, Victor Argueta, Andert Rosingh, Femke J H B van Aerde-van Nunen, Griselda Lopez, Herbert M Pinedo, Mark Schiffman, Michael Dean, Roberto Orozco
BACKGROUND: A low cost and accurate method for detecting high-risk (HR) human papillomavirus (HPV) is important to permit HPV testing for cervical cancer prevention. We used a commercially available HPV method (H13, Hybribio) which was documented to function accurately in a reduced volume of cervical specimen to determine the most prevalent HPV types and the distribution of HPV infections in over 1795 cancer-free women in Guatemala undergoing primary screening for cervical cancer by cytology...
May 15, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29764277/human-papillomavirus-hpv-is-absent-in-branchial-cleft-cysts-of-the-neck-distinguishing-them-from-hpv-positive-cystic-metastasis
#2
Lars Sivars, David Landin, Marzia Rizzo, Linnea Haeggblom, Cinzia Bersani, Eva Munck-Wikland, Anders Näsman, Tina Dalianis, Linda Marklund
BACKGROUND: Distinguishing branchial cleft cysts (BCCs) from cystic metastases of a human papillomavirus (HPV) positive oropharyngeal squamous cell carcinoma (OPSCC) is challenging. Fine needle aspirates (FNAs) from cystic metastasis may be non-representative, while reactive squamous cells from BCC can be atypic. Based on cytology and with the support of HPV DNA positivity many centers treat cystic metastasis oncological and thus patients are spared neck dissection. To do so safely, one must investigate whether HPV DNA and p16INK4a overexpression is found exclusively in cystic metastases and not in BCC...
May 15, 2018: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/29752912/association-between-viral-loads-of-different-oncogenic-human-papillomavirus-types-and-the-degree-of-cervical-lesions-in-the-progression-of-cervical-cancer
#3
Wei Wang, Xian-Hui Zhang, Mei Li, Chong-Hua Hao, Zhi-Min Zhao, Hong-Ping Liang
OBJECTIVE: In this study we determined the frequency of the most prevalent human papillomavirus (HPV) types in China and evaluated the association between viral loads of different oncogenic HPV types and the severity of disease. METHODS: We enrolled 15,518 women for this study and 3199 of them (20.61%) were identified as positive by a PCR assay, that can simultaneously quantify and genotype HPV. RESULTS: The viral loads of HPV 16, 31, 35, 52, 58, 39, and 56 were lower for women with normal cytology compared to those with disease progression; viral loads were not appreciable for HPV 33, 18, 45, 59, 68, 53, 66, and 51...
May 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29736316/hpv16-viral-load-and-physical-state-measurement-as-a-potential-immediate-triage-strategy-for-hr-hpv-infected-women-a-study-in-644-women-with-single-hpv16-infections
#4
Anna Manawapat-Klopfer, Lisa Wang, Juliane Haedicke-Jarboui, Frank Stubenrauch, Christian Munk, Louise T Thomsen, Peter Martus, Susanne K Kjaer, Thomas Iftner
High genome copy number (viral load) of human papillomavirus (HPV) is being discussed as a risk factor for high-grade cervical lesions. However, conflicting data about the integration status or viral load of the virus as risk factors for prevalent high-grade squamous intraepithelial lesions (HSIL) are found in the literature. To investigate whether viral load and/or integration status are indicative for prevalent ASCUS/LSIL or HSIL, we determined the HPV16 viral load and the physical state of the genome in 644 women with single HPV16 infections stratified by their cytology results from a large Danish population-based cohort consisting of 40,399 women...
2018: American Journal of Cancer Research
https://www.readbyqxmd.com/read/29699549/evaluation-of-human-papillomavirus-testing-and-visual-inspection-for-cervical-cancer-screening-in-rwanda
#5
M Chantal Umulisa, Silvia Franceschi, Iacopo Baussano, Vanessa Tenet, Mathilde Uwimbabazi, Belson Rugwizangoga, Daniëlle A M Heideman, Anne M Uyterlinde, Teresa M Darragh, Peter J F Snijders, Felix Sayinzoga, Gary M Clifford
BACKGROUND: A pilot screening campaign in Rwanda, based on careHPV-testing followed by visual inspection with acetic acid triage (careHPV+VIA triage), was evaluated against other WHO-recommended screening options, namely HPV screen-and-treat and VIA screen-and-treat. METHODS: 764 women aged 30-69 underwent at visit 1: i) VIA, and cervical cell collection for ii) careHPV in Rwanda, and iii) liquid-based cytology and GP5+/6+ HR-HPV PCR in The Netherlands. All 177 women positive by VIA, careHPV and/or PCR were recalled, of whom 84% attended...
April 24, 2018: BMC Women's Health
https://www.readbyqxmd.com/read/29678360/validation-of-a-new-hpv-self-sampling-device-for-cervical-cancer-screening-the-cervical-and-self-sample-in-screening-cassis-study
#6
Mariam El-Zein, Sheila Bouten, Karolina Louvanto, Lucy Gilbert, Walter Gotlieb, Robert Hemmings, Marcel A Behr, Eduardo L Franco, Victoria Liang, Claudia Martins, Silvy Duarte, Natalia Sarban, Patricia Geddes, Ana Massa, Kathrin Samios, Siham Aboufadl, Sophie Verdon, Cynthia Pereria, Isabelle Lacroix
OBJECTIVE: We compared the self-sampling performance of the newly designed HerSwab™ device with a physician-collected cervical sample and another self-sample using the cobas® PCR Female swab for the detection of cervical intraepithelial neoplasia (CIN) and cancer. METHODS: Women referred for colposcopy at McGill University affiliated hospital clinics collected two consecutive self-samples, one with HerSwab™ and one with cobas® swab, after receiving instructions...
April 17, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29668478/correlation-of-htert-expression-with-cervical-cytological-abnormalities-and-human-papillomavirus-infection
#7
Vjosa A Zejnullahu, Valon A Zejnullahu, Slavica Josifovska, Nikola Vukovik, Kiril Pakovski, Sasho Panov
Telomerase Reverse Transcriptase (TERT) is the main catalytic sub-unit of telomerase, a reverse transcriptase enzyme. Telomerase expression is regulated at many levels, with numerous studies suggesting that up-regulation of human TERT gene (hTERT) at transcriptional level results in immortal cell phenotype associated with cancer. The aim of this study is to determine the correlation between hTERT expression and different cervical precursor lesions, as well as with cervical cancer in patients with confirmed Human papillomavirus (HPV) infection...
December 1, 2017: Prilozi (Makedonska Akademija Na Naukite i Umetnostite. Oddelenie za Medicinski Nauki)
https://www.readbyqxmd.com/read/29664764/epstein-barr-virus-high-risk-human-papillomavirus-and-abnormal-cervical-cytology-in-a-prospective-cohort-of-african-female-sex-workers
#8
Jennifer E Cameron, Anne F Rositch, Nadja A Vielot, Nelly R Mugo, Jessie K L Kwatampora, Wairimu Waweru, Aubrey E Gilliland, Michael E Hagensee, Jennifer S Smith
BACKGROUND: High oncogenic-risk human papillomavirus (hrHPV) is necessary, although insufficient, to promote cervical cancer. Like HPV, Epstein-Barr virus (EBV) is a common pathogen with the capacity to promote epithelial neoplasms. We examined the association between cervical EBV, hrHPV, and cytology in female sex workers in Nairobi, Kenya. METHODS: Women (n=332) with known cervical cytology and hrHPV mRNA results were evaluated for cervical EBV DNA by conventional PCR...
April 17, 2018: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29608253/the-direct-and-indirect-association-of-cervical-microbiota-with-the-risk-of-cervical-intraepithelial-neoplasia
#9
Chaoting Zhang, Ying Liu, Weijiao Gao, Yaqi Pan, Yunong Gao, Jing Shen, Hongchao Xiong
Cervical microbiota composition is associated with cervical HPV infection and CIN severity. Previous studies only assessed the total association between cervical microbiota and HPV infections or CINs, and yet no study reported the direct and indirect associations between cervical microbiota and CINs mediated by HPV infection, respectively. The aim of this study was to investigate the direct and indirect associations between microbiotas and CIN severity. Cervical microbiota of 126 women with CIN 1- (normal cytology and CIN 1) and 40 with CIN 2+ (CIN 2 and CIN 3) were analyzed using Illumina sequencing based on the 16S rRNA gene...
April 2, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29590262/pcr-rflp-assay-as-an-option-for-primary-hpv-test
#10
L Golfetto, E V Alves, T R Martins, T C M Sincero, J B S Castro, C Dannebrock, J G Oliveira, J E Levi, A S C Onofre, M L Bazzo
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24...
March 26, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29587796/human-papilloma-virus-correlates-of-high-grade-cervical-dysplasia-in-hiv-infected-women-in-mombasa-kenya-a-cross-sectional-analysis
#11
Sonia Menon, Stanley Luchters, Rodolfo Rossi, Steven Callens, Mandaliya Kishor, Johannes Bogers, Davy Vanden Broeck
BACKGROUND: Women living with HIV are at increased risk to be co-infected with HPV, persistent high-risk (HR) human papillomavirus (HPV) infection and increased HR HPV viral load, which make them more at risk for cervical cancer. Despite their inherent vulnerability, there is a scarcity of data on potential high risk (pHR) and HR HPV genotypes in HIV- infected women with cervical dysplasia and HPV-type specific viral load in this population in Sub Saharan Africa. The aim of this analysis of HIV-infected women was to explore the virological correlates of high-grade cervical dysplasia (CIN 2+) in HIV-infected women, thereby profiling HPV genotypes...
March 27, 2018: Virology Journal
https://www.readbyqxmd.com/read/29571035/prevalence-and-age-wise-distribution-of-human-papillomavirus-type-16-18-infections-among-hospital-screened-women-of-a-peri-urban-area-in-west-bengal-impact-of-socio-demographic-factors
#12
Amrapali Bhattacharya, Shrinka Sen, Paramita Mandal, Sweta Sharma Saha, Somosree Sarkar, Om Prakash Pathak, Lena Biswas, Jayeeta Roy, Rimpa Banerjee, Ranita Roy Chowdhury, Manidip Pal, Ankur Mukherjee, Sharmila Sengupta
BACKGROUND: We undertook the current study on cervical Human Papillomavirus (HPV) prevalence along with cytology in women visiting the Gynecology Out-patient Department of a hospital for common gynecological ailments, subsequent to our earlier population-based study on HPV prevalence from India. METHODS: We analyzed data on cervical-cytology (Pap smears) and PCR-based molecular detection of HPV infection along with socio-demographic variables (N = 696). RESULTS: We identified 36...
March 20, 2018: Cancer Epidemiology
https://www.readbyqxmd.com/read/29555600/identifying-populations-most-susceptible-to-get-benefit-from-broadening-the-scope-for-prevention-of-cervical-cancer-example-from-uruguay
#13
Víctor-Guillermo Sequera, Marisa Mena, Malen Hollmann, Estefani Mani, Viviana Ramas, Sylvia Bonilla, Alicia Guerra, Fernando Borgia
OBJECTIVES: To identify factors associated with high-risk human papillomavirus (HPV) infection and high grade squamous intraepithelial lesion (HSIL) among a high-risk group of HPV-unvaccinated women in Montevideo. METHODS: Participants completed a questionnaire on socio-demographics, sexual behavior and gynecological history and received a gynecological examination. HPV DNA was detected by PCR using MY09/11 primers. Logistic regression analyses were performed to identify factors associated with high-risk HPV infection and HSIL...
March 16, 2018: Papillomavirus Research
https://www.readbyqxmd.com/read/29549429/integrated-morphologic-and-molecular-analysis-of-trichomonas-vaginalis-mycoplasma-hominis-and-human-papillomavirus-using-cytologic-smear-preparations
#14
I Nikas, A Hapfelmeier, M Mollenhauer, D Angermeier, M Bettstetter, R Götz, M Schmidmayr, V Seifert-Klauss, A Muckenhuber, U Schenck, Gregor Weirich
Pathogenic microbes may colonize the female genital tract via sexual transmission and cause health issues like inflammation or malignancy, summarized as sexually transmitted disease (STD). A major representative of such pathogens is Trichomonas vaginalis (T.v.), whose role in the etiology of cervical cancer remains elusive. Traditional morphologic screening of cervical smears is able to detect T.v., although its identification may be complicated by look-alikes such as degenerated granulocytes and basal cells...
March 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29544661/vaginosis-associated-bacteria-and-its-association-with-hpv-infection
#15
Pablo Romero-Morelos, Cindy Bandala, Julián Jiménez-Tenorio, Mariana Valdespino-Zavala, Miriam Rodríguez-Esquivel, Reyna Anaid Gama-Ríos, Artfy Bandera, Mónica Mendoza-Rodríguez, Keiko Taniguchi, Daniel Marrero-Rodríguez, Ricardo López-Romero, Eva Ramón-Gallegos, Mauricio Salcedo
BACKGROUND AND OBJECTIVE: Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and Gardnerella vaginalis has been suggested as potential risk factor for cervical lesions and cervical cancer. MATERIAL AND METHODS: DNA from 177 cervical scraping samples was studied: 104 belonged to women without cytological or colposcopic alterations and 73 samples from precursor lesions with previous human papillomavirus (HPV) infection history...
March 12, 2018: Medicina Clínica
https://www.readbyqxmd.com/read/29512809/the-prevelance-of-human-papillomavirus-hpv-genotypes-detected-by-pcr-in-women-with-normal-and-abnormal-cervico-vaginal-cytology
#16
Fatma Beyazit, Fatma Sılan, Meryem Gencer, Buket Aydin, Barış Paksoy, Mesut A Unsal, Ozturk Ozdemir
OBJECTIVES: Cervical cancer is the second most common type of cancer for women worldwide with a great proportion proved to be related to human papillomavirus (HPV) infection. As infection with HPV is the strongest risk factor for cervical neoplasia, detection of HPV genotypes in cervical and vaginal specimens of women with normal and abnormal cytology seems to be of paramount importance in cervical cancer screening. The objective of the study is to evaluate the prevalence and HPV genotypes among women with normal or abnormal Pap smear tests...
2018: Ginekologia Polska
https://www.readbyqxmd.com/read/29487007/mir-21-5p-mir-34a-and-human-telomerase-rna-component-as-surrogate-markers-for-cervical-cancer-progression
#17
Yue Zhu, Ying Han, Tian Tian, Peihong Su, Guan Jin, Juan Chen, Yungui Cao
OBJECTIVE: This study aimed to demonstrate the predictive value of miR-21-5p, miR-34a, and human telomerase RNA component (hTERC) in cervical cancer (CC) development and evaluated their potential possibility for future clinical applications. METHODS: Specimens were collected from the normal cervix, cervical intraepithelial neoplasia (CIN) I, CIN II/III, cervical squamous cell carcinoma. Cytological evaluations and histopathologic examinations were conducted in all subjects, along with the assessment of human papillomavirus (HPV) DNA...
March 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29466435/separate-analysis-of-human-papillomavirus-e6-and-e7-messenger-rnas-to-predict-cervical-neoplasia-progression
#18
Shuling Liu, Takeo Minaguchi, Bouchra Lachkar, Shuang Zhang, Chenyang Xu, Yuri Tenjimbayashi, Ayumi Shikama, Nobutaka Tasaka, Azusa Akiyama, Manabu Sakurai, Sari Nakao, Hiroyuki Ochi, Mamiko Onuki, Koji Matsumoto, Hiroyuki Yoshikawa, Toyomi Satoh
A few studies previously suggested that human papillomavirus (HPV) E6 messenger RNA (mRNA) may exist uniformly in all grades of cervical intraepithelial neoplasia (CIN), whereas the detection rate of E7 mRNA may increase with disease progression from low-grade CIN to invasive carcinoma. The aim of this study was to clarify the different roles of E6 and E7 mRNAs in cervical carcinogenesis. The presence of each E6 and E7 mRNA was analyzed in 171 patients with pathologically-diagnosed CIN or cervical carcinoma...
2018: PloS One
https://www.readbyqxmd.com/read/29454382/high-risk-human-papillomavirus-prevalence-and-genotype-distribution-among-women-infected-with-hiv-in-manaus-amazonas
#19
Monique Figueiredo Teixeira, Meritxell Sabidó, André Luiz Leturiondo, Cynthia de Oliveira Ferreira, Kátia Luz Torres, Adele Schwartz Benzaken
BACKGROUND: Human immunodeficiency virus (HIV)-positive women have a high prevalence of human papillomavirus (HPV), and are infected with a broader range of HPV types than HIV-negative women. We aimed to determine the prevalence of cervical cytologic abnormalities, high-risk (HR)-HPV prevalence, type distribution according to the severity of cervical lesions and CD4 cell count and identify factors associated with HR-HPV infection among women living with HIV in Manaus, Amazonas. METHODS: We enrolled 325 women living with HIV that attended an infectious diseases referral hospital...
February 17, 2018: Virology Journal
https://www.readbyqxmd.com/read/29453769/human-papilloma-virus-genotyping-for-the-cross-sectional-and-longitudinal-probability-of-developing-cervical-intraepithelial-neoplasia-grade-2-or-more
#20
Annarosa Del Mistro, Rachael Adcock, Francesca Carozzi, Anna Gillio-Tos, Laura De Marco, Salvatore Girlando, Raffaella Rizzolo, Helena Frayle, Morena Trevisan, Cristina Sani, Elena Burroni, Paolo Giorgi Rossi, Jack Cuzick, Guglielmo Ronco
Human papilloma virus (HPV) testing is more sensitive but less specific than cytology. We evaluated stand-alone genotyping as a possible triage method. During a multicentre randomised controlled trial comparing HPV testing to conventional cytology, HPV-positive women were referred to colposcopy and followed up if no high-grade lesion was detected. HPV-positive samples were genotyped by GP5+/GP6+ primed polymerase chain reaction followed by reverse line blot. Genotypes were hierarchically ordered by positive predictive value (PPV) for CIN grade 2 or more (CIN2+), and grouped by cluster analysis into three groups (A, B and C in decreasing order)...
February 17, 2018: International Journal of Cancer. Journal International du Cancer
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