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HPV PCR cytology

Nicolas F Schlecht, Angela Diaz, Viswanathan Shankar, Arnold H Szporn, Maoxin Wu, Anne Nucci-Sack, Ken Peake, Howard D Strickler, Robert D Burk
BACKGROUND:  Uptake of the human papillomavirus (HPV) vaccine in the United States is slow, and the real-world effectiveness of the vaccine has not been assessed in high-risk adolescent populations. METHODS:  We conducted a longitudinal study of 1139 sexually-active, inner-city adolescent women receiving the 3-dose quadrivalent (4vHPV) vaccine. Cervical and anal specimens collected semi-annually were tested using a L1-PCR assay. Post-vaccination incidence of 4vHPV vaccine and non-vaccine HPV types, and risk of cervical cytological abnormalities, were assessed in relation to time-to-completion of all three vaccine doses...
October 12, 2016: Journal of Infectious Diseases
Jinhyuk Choi, Chungyeul Kim, Hye Seung Lee, Yoo Jin Choi, Ha Yeon Kim, Jinhwan Lee, Hyeyoon Chang, Aeree Kim
Background: Human papillomavirus (HPV) is a well-established oncogenic virus of cervical, anogenital, and oropharyngeal cancer. Various subtypes of HPV have been detected in 0% to 60% of breast cancers. The roles of HPV in the carcinogenesis of breast cancer remain controversial. This study was performed to determine the prevalence of HPV-positive breast cancer in Korean patients and to evaluate the possibility of carcinogenic effect of HPV on breast. Materials and Methods: Meta-analysis was performed in 22 case-control studies for HPV infection in breast cancer...
October 10, 2016: Journal of Pathology and Translational Medicine
Pamela Boundzanga Moussavou, Ismaël Hervé Koumakpayi, Andriniaina Andy Nkili-Meyong, Ingrid Labouba, Ulrich Bisvigou, Junie K Chansi, Corinne Engohan-Aloghe, Frederic Dissanami, Nathalie Ambounda, Anne-Sophie Delannoy-Vieillard, Laure Diancourt, Dieudonne Nkoghe, Eric M Leroy, Ernest Belembaogo, Nicolas Berthet
BACKGROUND: The human papillomavirus (HPV) is the causative agent of cervical cancer, which is the leading cancer-related cause of death for women in Sub-Saharan Africa. In 2013, the Gabonese Ministry of Health and the Sylvia Bongo Ondimba Foundation implemented cervical cancer screening programs based on the detection of cancerous lesions by visual inspection with acetic acid and/or Lugol's iodine (VIA/VILI). This pilot study was set up to determine the HPV profile and analyze the nucleotide sequence variation of HPV16 circulating in patients with cervical abnormalities detected by VIA/VILI testing...
2016: Infectious Agents and Cancer
Xiaotong He, Innocent O Maranga, Anthony W Oliver, Peter Gichangi, Lynne Hampson, Ian N Hampson
The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV-ve) Kenyan women...
2016: Viruses
Idanya Serafín-Higuera, Olga Lilia Garibay-Cerdenares, Berenice Illades-Aguiar, Eugenia Flores-Alfaro, Marco Antonio Jiménez-López, Pavel Sierra-Martínez, Luz Del Carmen Alarcón-Romero
BACKGROUND: Cervical cancer (CC) is the fourth most common cancer in women worldwide with an estimated 528,000 new cases in 2012. The same year México had an incidence of 13,960 and a mortality of 4769 cases. There are several diagnosis methods of CC; among the most frequents are the conventional Pap cytology (Pap), colposcopy, and visual inspection with acetic acid (VIA), histopathological examination, tests of imaging and detection of high-risk papilloma virus (HR-HPV) with molecular tests (PCR, hybridization, sequencing)...
2016: Proteome Science
O M Kolawole, K T Olatunji, K A Durowade
BACKGROUND: Human papillomavirus (HPV) has been implicated as one of the principal causes of cervical cancer, which is the second highest cause of cancer deaths among Nigerian women. OBJECTIVE: This study was aimed at determining the presence of HPV DNA in abnormal cervical cytology of a group of women who were screened using Papanicolaou staining technique. METHODOLOGY: A total of 200 women attending the Obstetrics and Gynaecology clinic of the Federal Teaching Hospital, Ido-Ekiti, were screened by means of conventional Pap smear screening, while positive samples underwent molecular analyses by means of DNA isolation techniques and polymerase chain reaction (PCR)...
2016: Journal of Preventive Medicine and Hygiene
Xiang-Xing Zeng, Li-Xiang Yan, Xiu-Xia Huang, Cai-Hua He, Wei-Guo Liu, Wen-Qing Yuan, Yan-Ping Qiu, Zhi-Xiang Liu
BACKGROUND: Human papillomavirus (HPV) infection is the primary risk factor for cervical cancer. HPV genotypes are associated with varying degrees of pathogenicity. To better formulate strategies for cervical cancer prevention, we investigated the population-specific distribution of HPV genotypes, including those with high carcinogenicity. METHODS: From January to December 2012, a cervical cancer-screening program for HPV infection in Hakka women of Heyuan City Guangdong province was conducted...
August 2016: Annals of Translational Medicine
Giuseppa Visalli, Monica Currò, Alessio Facciolà, Romana Riso, Placido Mondello, Pasqualina Laganà, Angela Di Pietro, Isa Picerno, Pasquale Spataro
BACKGROUND: The human papilloma viruses (HPVs) are DNA viruses associated with benign and malignant lesions of skin and mucous membranes. The HPVs has been implicated as the cause of virtually all cervical cancers worldwide but studies showed that these viruses can cause numerous cancers in several tissues including Oral Squamous Cell Carcinoma (OSCC). At least 90 % of HPV-positive OSCCs are associated with high-risk (or oncogenic) HPV-16 and oral infection confers an approximate 50-fold increase in risk for HPV-positive OSCC...
2016: Infectious Agents and Cancer
Y P Wong, S H Abdul Raub, A Z H Mohd Dali, F Kassim, V Visvalingam, Z Zakaria, M A Kamaluddin, S Noor Akmal
Human papillomavirus (HPV) is a necessary cause of cervical cancer and its precursors. Increased expression of high-risk hrHPV viral oncogenes in abnormal cells might increase the expression of p16INK4a. We aimed to determine the role of p16INK4a in detecting hrHPV-transformed epithelial cells in liquid-based cervical cytology, and compared the results with hrHPV DNA testing by realtime polymerase chain reaction (RT-PCR). Fifty-seven cytological samples were tested for p16INK4a immunomarker and hrHPV DNA. Test performance of both tests was determined by comparing sensitivity, specificity and predictive values using available histological follow-up data as gold standard...
August 2016: Malaysian Journal of Pathology
Fridolin Mujuni, Mariam M Mirambo, Peter Rambau, Korn Klaus, Muller Andreas, Dismas Matovelo, Mtebe Majigo, Christa Kasang, Stephen E Mshana
BACKGROUND: High risk (HR) human papilloma Virus (HPV) genotypes have been associated with cervical cancer. In Tanzania there is a limited data on the epidemiology of HPV and genotypes distribution among HIV infected women. Here we document varieties of HPV genotypes associated with cervical squamous intraepithelial lesions (SIL) among HIV- infected women at Bugando Medical Centre, Mwanza-Tanzania. METHODS: A cross sectional hospital based study involving HIV infected women was conducted between August and October, 2014...
2016: Infectious Agents and Cancer
Monserrat Balanda, Andrea Quiero, Nicolás Vergara, Gloria Espinoza, Héctor San Martín, Giovanna Rojas, Eugenio Ramírez
Cervical cancer is the fourth most common malignancy in women worldwide. In Chile, cervical cancer is the second leading cause of death among women of reproductive age, causing more than 600 deaths annually. This study was carried out to determine the burden and confirm the predominant human papillomavirus (HPV) genotypes among women presenting for cervical cancer screening in public health services in Chile. Women aged 18-64 years residing in the north and central areas covered by six primary care centers of Santiago, Chile, were invited to participate from March 2014 to August 2015...
August 18, 2016: Medical Microbiology and Immunology
Andre Lp de Abreu, Fabricia Gimenes, Natalia Malaguti, Monalisa W Pereira, Nelson S Uchimura, Marcia El Consolaro
To determine the prevalence of human papillomavirus (HPV) among women with atypical squamous cells of undetermined significance (ASC-US) referred to colposcopy and the implications for clinical management in low- and middle-income countries (LMIC), the present study was conducted. We included 200 women living in Maringa÷Brazil referred to colposcopy service between August 2012 and March 2013 due to an abnormal cytology from ASC-US until high-grade intraepithelial lesion (HSIL). HPV was detected and genotyped by polymerase chain reaction (PCR)...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Anna Manawapat-Klopfer, Louise T Thomsen, Peter Martus, Christian Munk, Rainer Russ, Hans Gmuender, Kirsten Frederiksen, Juliane Haedicke-Jarboui, Frank Stubenrauch, Susanne K Kjaer, Thomas Iftner
Women persistently infected with human papillomavirus (HPV) type 16 are at high risk for development of cervical intraepithelial neoplasia grade 3 or cervical cancer (CIN3+). We aimed to identify biomarkers for progression to CIN3+ in women with persistent HPV16 infection. In this prospective study, 11,088 women aged 20-29 years were enrolled during 1991-1993, and re-invited for a second visit two years later. Cervical cytology samples obtained at both visits were tested for HPV DNA by Hybrid Capture 2 (HC2), and HC2-positive samples were genotyped by INNO-LiPA...
2016: American Journal of Cancer Research
Norbert Varga, Johanna Mózes, Helen Keegan, Christine White, Lynne Kelly, Loretto Pilkington, Márta Benczik, Schaff Zsuzsanna, Gábor Sobel, Róbert Koiss, Edit Babarczi, Miklos Nyíri, Laura Kovács, Sebe Attila, Borbála Kaltenecker, Adrienn Géresi, Adrienn Kocsis, John O'Leary, Cara M Martin, Csaba Jeney
In the era of primary vaccination against HPV and at the beginning of the low prevalence of cervical lesions, introduction of screening methods that can distinguish between low- and high-grade lesions is necessary in order to maintain the positive predictive value of screening. This case-control study included 562 women who attended cervical screening or were referred for colposcopy and 140 disease free controls, confirmed by histology and/or cytology. The cases were stratified by age. Using routine exfoliated liquid based cytological samples RT-PCR measurements of biomarker genes, high-risk HPV testing and liquid based cytology were performed and used to evaluate different testing protocols including sets of genes/tests with different test cut-offs for the diagnostic panels...
August 6, 2016: Pathology Oncology Research: POR
Lina Wang, Pengyan Wang, Yan Ren, Jingyun Du, Jianjun Jiang, Xuesong Jia, Chuangfu Chen, Yuanzhi Wang
Multiple human papillomavirus (HPV) genotypes often coexist within the cervical epithelia and are frequently detected together in various grades of the cervical neoplasia. To date, only a few reports exist on multiple HPV infections of HPV in Xinjiang Uygur Autonomous Region (XUAR). In the present study, we investigated the prevalence of High-Risk HPV (HR-HPV) genotypes and multiple infections. Cervical cytology samples were collected from 428 women who presented cervical abnormalities. Genotyping of HPV was performed by polymerase chain reaction-sequencing based typing (PCR-SBT) using consensus primers and specific primers...
2016: PloS One
Delia Savone, Angela Carrone, Lucia Riganelli, Lucia Merlino, Pasquale Mancino, Pierluigi Benedetti Panici
This systematic review of 43 studies aims to evaluate the absolute and relative sensitivity and specificity of p16INK4a with regard to uterine cervix lesions, describing innovations and techniques for the detection of high-grade cervical dysplasia and allowing correct treatment. Studies were identified in the PubMed database up to March 2015. The keywords hrHPV, p16INK4a gene, and uterine cervical disease (MeSH terms) were used. Only English-language articles were included. We considered retrospective and prospective studies that assessed p16INK4a or p16INK4a/Ki67 staining, with or without HPV-DNA testing (HC2/PCR) as a comparator test, in cytological/histological specimens for which the diagnosis of ASCUS, LSIL or HSIL was verified with a reference standard...
October 13, 2016: Tumori
André Lp de Abreu, Natália Malaguti, Raquel P Souza, Nelson S Uchimura, Érika C Ferreira, Monalisa W Pereira, Maria Db Carvalho, Sandra M Pelloso, Marcelo G Bonini, Fabrícia Gimenes, Marcia El Consolaro
The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods...
2016: American Journal of Cancer Research
Dejan R Nonato, Rosane R F Alves, Andrea A Ribeiro, Vera A Saddi, Kelly D Segati, Keila P Almeida, Yanna A R de Lima, Walmirton B D'Alessandro, Silvia H Rabelo-Santos
BACKGROUND: Human papillomavirus and Chlamydia trachomatis share the same route of sexual transmission and possess similar risk factors, indicating that coinfection may act synergistically in the induction of epithelial cell abnormalities. OBJECTIVE: This study aimed to determine the prevalence of human papillomavirus and Chlamydia trachomatis in adolescents and young women and identify factors associated with coinfection. STUDY DESIGN: This cross-sectional study included 276 female participants, aged 15-24 years, who were sexually active...
July 8, 2016: American Journal of Obstetrics and Gynecology
I Heard, K Cuschieri, D T Geraets, W Quint, M Arbyn
BACKGROUND: The benefit of HPV testing for cervical cancer screening and disease management has been shown in many recent studies and is part of several new evidence-based guidelines. Assessment of emerging HPV tests in this context is essential, using well-annotated samples, such as those generated via the Validation of Genotyping Tests-HPV (VALGENT) framework. OBJECTIVE: Our aim was to assess the PapilloCheck HPV assay in terms of absolute and relative accuracy for primary cervical cancer screening, using a standard comparator test (GP5+/6+EIA)already validated in randomised trials...
August 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Fidele Ngabo, Silvia Franceschi, Iacopo Baussano, M Chantal Umulisa, Peter J F Snijders, Anne M Uyterlinde, Fulvio Lazzarato, Vanessa Tenet, Maurice Gatera, Agnes Binagwaho, Gary M Clifford
BACKGROUND: Cervical cancer is the most common female cancer in Rwanda that, in 2011, became the first African country to implement a national vaccination programme against human papillomavirus (HPV). METHODS: To provide a robust baseline for future evaluations of vaccine effectiveness, cervical cell specimens were obtained from 2508 women aged 18-69 years from the general population in Kigali, Rwanda, during 2013/14. 20 % of women were HIV-positive. Samples were used for liquid-based cytology and HPV testing (44 types) with GP5+/6+ PCR...
2016: BMC Infectious Diseases
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