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Epigallocatechin gallate

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https://www.readbyqxmd.com/read/28334752/monitoring-the-consistency-quality-and-antioxidant-activity-of-da-hong-pao-teas-by-hplc-fingerprinting
#1
Lin Wang, Ting Yan, Lanping Yang, Kexia Zhang, Jingming Jia
In the present study, a high performance liquid chromatography (HPLC) method was developed and validated for fingerprint profiling to evaluate the quality consistency of the Da Hong Pao tea. The chromatographic separation was performed on a USA Waters C18 column (4.6 mm × 250 mm, 5 μm, Waters-XBridge Shield RP 18) with gradient elution. The column temperature was maintained at 30°C and the detection wavelength was set at 278 nm. The results demonstrated that the developed HPLC method achieved the desired linearity, precision, accuracy and limit of quantification...
February 18, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28329447/formation-of-a-multi-ligand-complex-of-bovine-serum-albumin-with-retinol-resveratrol-and-epigallocatechin-3-gallate-for-the-protection-of-bioactive-components
#2
Yi Wu, Hao Cheng, Yantao Chen, Lingyun Chen, Zheng Fang, Li Liang
Clarification of the interaction mechanisms between proteins and bioactive components is important to develop effective carriers for encapsulation and protection of bioactive components. Bovine serum albumin (BSA), a globular protein in serum and milk, contains multiple sites to bind a variety of low-molecular-weight molecules, forming protein-mono-ligand complexes. In this study, the interactions of BSA with retinol, resveratrol and/or (-)-epigallocatechin-3-gallate (EGCG) were investigated by using fluorescence, circular dichroism and molecular docking techniques...
March 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28328549/epigallocatechin-gallate-inhibits-macaque-sevi-mediated-enhancement-of-siv-or-shiv-infection
#3
Run-Hong Zhou, Le Guo, Jin-Biao Liu, Hang Liu, Wei Hou, Tong-Cui Ma, Xu Wang, Jian-Guo Wu, Li Ye, Wen-Zhe Ho, Jie-Liang Li
BACKGROUND: Human semen contains a factor that can enhance HIV infection up to 10^5-fold in cultures. This factor is termed SEVI (Semen-derived Enhancer of Virus Infection), which is composed of proteolytic fragments (PAP248-286) from prostatic acid phosphatase (PAP) in semen. Here, we examined whether macaque SEVI can facilitate simian immunodeficiency virus (SIV) or chimeric simian/human immunodeficiency virus (SHIV) infection. We also studied the effect of EGCG on macaque SEVI-mediated SIV or SHIV enhancement...
March 22, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28325826/a-phase-ii-randomized-double-blind-presurgical-trial-of-polyphenon-e-in-bladder-cancer-patients-to-evaluate-pharmacodynamics-and-bladder-tissue-biomarkers
#4
Jason R Gee, Daniel R Saltzstein, KyungMann Kim, Jill M Kolesar, Wei Huang, Thomas C Havighurst, Barbara W Wollmer, Jeanne Stublaski, Tracy Downs, Hasan Mukhtar, Margaret House, Howard L Parnes, Howard H Bailey
We performed a phase 2 pharmacodynamic, prevention trial of Polyphenon E® (a green tea polyphenol formulation primarily consisting of epigallocatechin gallate (EGCG)) in patients prior to bladder cancer surgery. Patients with a bladder tumor were randomized to receive Polyphenon E® containing either 800 or 1200 mg of EGCG or placebo for 14 to 28 days prior to TURBT or cystectomy. The primary objective was to compare the post-intervention EGCG tissue levels in patients receiving Polyphenon E® as compared to placebo...
March 21, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28325635/epigallocatechin-3-gallate-inhibits-bacterial-virulence-and-invasion-of-host-cells
#5
Lun K Tsou, Jacob S Yount, Howard C Hang
Increasing antibiotic resistance and beneficial effects of host microbiota has motivated the search for anti-infective agents that attenuate bacterial virulence rather than growth. For example, we discovered that specific flavonoids such as baicalein and quercetin from traditional medicinal plant extracts could attenuate Salmonella enterica serovar Typhimurium type III protein secretion and invasion of host cells. Here, we show epigallocatechin-3-gallate from green tea extracts also inhibits the activity of S...
March 10, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28323106/implications-of-epigallocatechin-3-gallate-in-cultured-human-sertoli-cells-glycolytic-and-oxidative-profile
#6
Tânia R Dias, Marco G Alves, Joaquina Silva, Alberto Barros, Mário Sousa, Susana Casal, Branca M Silva, Pedro F Oliveira
Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs)...
March 17, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28320327/metabolomics-analysis-reveals-the-metabolic-and-functional-roles-of-flavonoids-in-light-sensitive-tea-leaves
#7
Qunfeng Zhang, Meiya Liu, Jianyun Ruan
BACKGROUND: As the predominant secondary metabolic pathway in tea plants, flavonoid biosynthesis increases with increasing temperature and illumination. However, the concentration of most flavonoids decreases greatly in light-sensitive tea leaves when they are exposed to light, which further improves tea quality. To reveal the metabolism and potential functions of flavonoids in tea leaves, a natural light-sensitive tea mutant (Huangjinya) cultivated under different light conditions was subjected to metabolomics analysis...
March 20, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28317707/comparison-of-binding-interaction-between-%C3%AE-lactoglobulin-and-three-common-polyphenols-using-multi-spectroscopy-and-modeling-methods
#8
Jingjing Jia, Xin Gao, Minghao Hao, Lin Tang
Tea, coffee and fruit in dairy products are rich in polyphenols. The interaction mechanism between β-lactoglobulin (β-LG) and chlorogenic acid (CGA), ferulic acid (FA) and epigallocatechin-3-gallate (EGCG) was investigated. Fluorescence experiments proved that polyphenols quenched β-LG fluorescence strongly in static mode and EGCG had stronger binding affinity toward β-LG than CGA and FA. The main interaction force of EGCG binding with β-LG was different from CGA and FA. Furthermore, circular dichroism and fourier transform infrared data indicated that polyphenols changed β-LG secondary structure inducing a-helix to β-structures transition...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317253/correlation-between-the-potency-of-flavonoids-for-cytochrome-c-reduction-and-inhibition-of-cardiolipin-induced-peroxidase-activity
#9
Ricardo Lagoa, Alejandro K Samhan-Arias, Carlos Gutierrez-Merino
There are large differences between flavonoids to protect against apoptosis, a process in which cytochrome c (Cyt c) plays a key role. In this work, we show that 7 of 13 flavonoids studied have a capacity to reduce Cyt c similar or higher than ascorbate, the flavonols quercetin, kaempferol and myricetin, flavanol epigallocatechin-gallate, anthocyanidins cyanidin and malvidin, and the flavone luteolin. In contrast, the kaempferol 3(O)- and 3,4'(O)-methylated forms, the flavanone naringenin, and also apigenin and chrysin, had a negligible reducing capacity...
March 20, 2017: BioFactors
https://www.readbyqxmd.com/read/28317089/egcg-protects-against-homocysteine-induced-human-umbilical-vein-endothelial-cells-apoptosis-by-modulating-mitochondrial-dependent-apoptotic-signaling-and-pi3k-akt-enos-signaling-pathways
#10
Shumin Liu, Zhengwu Sun, Peng Chu, Hailong Li, Anil Ahsan, Ziru Zhou, Zonghui Zhang, Bin Sun, Jingjun Wu, Yalin Xi, Guozhu Han, Yuan Lin, Jinyong Peng, Zeyao Tang
Homocysteine (Hcy) induced vascular endothelial injury leads to the progression of endothelial dysfunction in atherosclerosis. Epigallocatechin gallate (EGCG), a natural dietary antioxidant, has been applied to protect against atherosclerosis. However, the underlying protective mechanism of EGCG has not been clarified. The present study investigated the mechanism of EGCG protected against Hcy-induced human umbilical vein endothelial cells (HUVECs) apoptosis. Methyl thiazolyl tetrazolium assay (MTT), transmission electron microscope, fluorescent staining, flow cytometry, western blot were used in this study...
March 19, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28315325/-epigallocatechin-3-gallate-stimulates-myogenic-differentiation-through-taz-activation
#11
A Rum Kim, Kyung Min Kim, Mi Ran Byun, Jun-Ha Hwang, Jung Il Park, Ho Taek Oh, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Muscle loss is a typical process of aging. Green tea consumption is known to slow down the progress of aging. Their underlying mechanisms, however, remain largely unknown. In this study, we investigated the effect of (-)-epigallocatechin-3-gallate (EGCG), a polyphenolic compound of green tea, on myogenic differentiation and found that EGCG significantly increases myogenic differentiation. After EGCG treatment, the expression of myogenic marker genes, such as myosin heavy chain, are increased through activation of TAZ, a transcriptional coactivator with a PDZ-binding motif...
March 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28300193/interplay-of-myosin-phosphatase-and-protein-phosphatase-2a-in-the-regulation-of-endothelial-nitric-oxide-synthase-phosphorylation-and-nitric-oxide-production
#12
Róbert Bátori, Bálint Bécsi, Dénes Nagy, Zoltán Kónya, Csaba Hegedűs, Zsuzsanna Bordán, Alexander Verin, Beáta Lontay, Ferenc Erdődi
The inhibitory phosphorylation of endothelial nitric oxide (NO) synthase (eNOS) at Thr497 (eNOS(pThr497)) by protein kinase C or RhoA-activated kinase is a major regulatory determinant of eNOS activity. The signalling mechanisms involved in the dephosphorylation of eNOS(pThr497) have not yet been clarified. This study identifies myosin phosphatase (MP) holoenzyme consisting of protein phosphatase-1 catalytic subunit (PP1c) and MP target subunit-1 (MYPT1) as an eNOS(pThr497) phosphatase. In support of this finding are: (i) eNOS and MYPT1 interacts in various endothelial cells (ECs) and in in vitro binding assays (ii) MYPT1 targets and stimulates PP1c toward eNOS(pThr497) substrate (iii) phosphorylation of MYPT1 at Thr696 (MYPT1(pThr696)) controls the activity of MP on eNOS(pThr497)...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298691/the-effective-and-selective-separation-of-epigallocatechin-gallate-by-molecularly-imprinted-chitosan-beads
#13
Xueqing Yu, Yingjun Jing, Nana Yin
The (-)-epigallocatechin gallate (EGCG) imprinted chitosan beads (EICBs) were fabricated for the effective and selective separation of EGCG. The EGCG molecules interacted with the amino groups of chitosan in the imprinting process, resulting in a highly porous structure of EICBs and more adsorption sites. Consequently, EICBs exhibited better adsorption performance than non-imprinted chitosan beads. The maximum adsorption capacity of EGCG onto EICBs reached 135.50 mg/g at 313 K. The imprinting factor of EICBs was 4...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28292023/green-tea-and-its-anti-angiogenesis-effects
#14
REVIEW
Bahman Rashidi, Mehrnoush Malekzadeh, Mohammad Goodarzi, Aria Masoudifar, Hamed Mirzaei
The development of new blood vessels from a pre-existing vasculature (also known as angiogenesis) is required for many physiological processes including embryogenesis and post-natal growth. However, pathological angiogenesis is also a hallmark of cancer and many ischaemic and inflammatory diseases. The pro-angiogenic members of the VEGF family (vascular endothelial growth factor family), VEGF-A, VEGF-B, VEGF-C, VEGF-D and placental growth factor (PlGF), and the related receptors, VEGFR-1, VEGFR-2 and VEGFR-3 have a central and decisive role in angiogenesis...
March 8, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28290302/the-modulatory-effect-of-epigallocatechin-3-o-3-o-methyl-gallate-egcg3%C3%A2-me-on-intestinal-microbiota-of-high-fat-diet-induced-obesity-mice-model
#15
Mei Cheng, Xin Zhang, Yingjie Miao, Jinxuan Cao, Zufang Wu, Peifang Weng
(-)-Epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) has exhibited multiple beneficial effects on the prevention of obesity in oolong tea. However, its absorption is relatively low, and the potential to be fully utilized is not completely elucidated. Therefore, with human flora-associated (HFA) mice model, the effect of EGCG3″Me on high fat diet-induced obesity was investigated by high-throughput sequencing. The shifts in relative abundance of the dominant taxa at the phylum, family and genus levels showed the dramatically effects of EGCG3″Me...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28290290/mucus-interactions-with-liposomes-encapsulating-bioactives-interfacial-tensiometry-and-cellular-uptake-on-caco-2-and-cocultures-of-caco-2-ht29-mtx
#16
Yang Li, Elena Arranz, Anilda Guri, Milena Corredig
Structuring of delivery matrices in foods aquires careful designing for optimal delivery and subsiquent absorption of the beneficial compounds in the gut. There has been quite improvement in mimicking digestion and absorption in vitro but as of yet little is understood on mucus interference in nutrient absorption Therefore in this study interactions of human intestinal mucus with milk and soy phospholipids liposomes carring hydrophilic (epigallocatechin-3-gallate) or hydrophobic (β-carotene) bioactive molecules were investigated...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28288871/flavan-3-ols-and-proanthocyanidins-from-limonium-brasiliense-inhibit-the-adhesion-of-porphyromonas-gingivalis-to-epithelial-host-cells-by-interaction-with-gingipains
#17
Angelo de Oliveira Caleare, Andreas Hensel, João Carlos Palazzo Mello, Andressa Blainski Pinha, Gean Pier Panizzon, Matthias Lechtenberg, Frank Petereit, Celso Vataru Nakamura
Porphyromonas gingivalis is a pathogen strongly involved in chronic and aggressive forms of periodontitis. Natural products, mainly polyphenols, have been described for advanced treatment of periodontitis by inhibition of the bacterial adhesion of P. gingivalis to the epithelial host cells. An acetone:water extract (LBE) from the rhizomes of Limonium brasiliense (Boiss.) Kuntze was tested under in vitro conditions for potential antiadhesive effects against P. gingivalis to human KB cells and for inhibition of the proteolytic activity of gingipains, the main virulence factor of P...
March 11, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28286519/erbb-proteins-as-molecular-target-of-dietary-phytochemicals-in-malignant-diseases
#18
REVIEW
Alexandru Filippi, Oana-Alina Ciolac, Constanța Ganea, Maria-Magdalena Mocanu
ErbB proteins overexpression, in both normal and mutated forms, is associated with invasive forms of cancer prone to metastasis and with stronger antiapoptotic mechanisms and therefore more challenging to treat. Downstream effectors of ErbB receptors mediating these phenotypic traits include MAPK, STAT, and PI3K/AKT/mTOR pathways. Various phytochemical compounds were studied for their large number of biological effects including anticancer activity. Among these compounds, epigallocatechin-3-gallate (EGCG), the main catechin from green tea leaves, and curcumin, component of the curry powder, constituted the object of numerous studies...
2017: Journal of Oncology
https://www.readbyqxmd.com/read/28286171/epigallocatechin-3-gallate-increases-autophagy-signaling-in-resting-and-unloaded-plantaris-muscles-but-selectively-suppresses-autophagy-protein-abundance-in-reloaded-muscles-of-aged-rats
#19
Hideyuki Takahashi, Yutaka Suzuki, Junaith S Mohamed, Takafumi Gotoh, Suzette L Pereira, Stephen E Alway
We have previously found that Epigallocatechin-3-gallate (EGCg), an abundant catechin in green tea, reduced apoptotic signaling and improved muscle recovery in response to reloading after hindlimb suspension (HS). In this study, we investigated if EGCg altered autophagy signaling in skeletal muscle of old rats in response to HS or reloading after HS. Fischer 344×Brown Norway inbred rats (age 34months) were given 1ml/day of purified EGCg (50mg/kg body weight), or the same sample volume of the vehicle by gavage...
March 7, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28285951/potential-role-of-green-tea-catechins-in-the-management-of-oxidative-stress-associated-infertility
#20
REVIEW
Shubhadeep Roychoudhury, Ashok Agarwal, Gurpriya Virk, Chak-Lam Cho
Reactive oxygen species (ROS) are present in low concentrations in the genital tracts of males and females. Excessive ROS lead to oxidative stress, which damages DNA, lipids and proteins. Such molecular changes result in compromised vitality, increased morphological defects and decreased sperm motility in the male. In the female, oxidative stress interferes with oocyte maturation, and may inhibit in-vitro maturation of the oocyte. Recently, green tea supplementation has been reported to possess properties that may improve the quality of male and female gametes largely due to the ability of catechin polyphenols to quench ROS...
February 27, 2017: Reproductive Biomedicine Online
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