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Epigallocatechin gallate

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https://www.readbyqxmd.com/read/28927122/mechanism-of-egcg-promoting-apoptosis-of-mcf-7-cell-line-in-human-breast-cancer
#1
Chao-You Huang, Zheng Han, Xi Li, Hui-Hua Xie, Shan-Shan Zhu
The aim of the present study was to investigate the effects of epigallocatechin-3-gallate (EGCG) on the apoptosis of the human MCF-7 cancer cell line and the underlying mechanism. MCF-7 cells were divided into the control group and EGCG groups. The proliferation of MCF-7 cells in the two groups was determined using MTT and apoptosis was examined using flow cytometry. Western blot analysis and qRT-PCR were used to analyze P53 and Bcl-2 expression levels. The silencing effect of specific siRNA was evaluated using RT-PCR and western blot analysis...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28925507/-epigallocatechin-3-gallate-and-ezh2-inhibitor-gsk343-have-similar-inhibitory-effects-and-mechanisms-of-action-on-colorectal-cancer-cells
#2
Le Ying, Feng Yan, Bryan R G Williams, Ping Xu, Xin Li, Yueling Zhao, Yiqun Hu, Yuefei Wang, Dakang Xu, Jing Dai
Epigallocatechin-3-gallate (EGCG) is a type of catechin. It exhibits excellent antioxidant effects and anti-tumor activities for cancer chemoprevention. The mechanism of anti-tumor effects of EGCG on different cancers has been studied for the past few decades, but remains controversial. To investigate the potential role that EGCG may play in the epigenetic regulation of colorectal cancer (CRC) cell line, we integrated bioinformatics analysis with experimental validation. We found that levels of the enhancer of zeste homolog 2 (EZH2) were significantly higher in CRC tissues compared to normal adjacent tissues, based on the Genomic Data Commons (GDC) data portal...
September 19, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28923363/anti-inflammatory-effects-of-flavonoids-in-neurodegenerative-disorders
#3
Carmela Spagnuolo, Stefania Moccia, Gian Luigi Russo
Neuroinflammation is one of the main mechanisms involved in the progression of several neurodegenerative diseases, such as Parkinson, Alzheimer, multiple sclerosis, amyotrophic lateral sclerosis and others. The activation of microglia is the main feature of neuroinflammation, promoting the release of pro-inflammatory cytokines and resulting in the progressive neuronal cell death. Natural compounds, such as flavonoids, possess neuroprotective potential probably related to their ability to modulate the inflammatory responses involved in neurodegenerative diseases...
September 7, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28914528/inhibition-of-human-and-rat-sucrase-and-maltase-activities-to-assess-anti-glycemic-potential-optimisation-of-the-assay-using-acarbose-and-polyphenols
#4
Alison Pyner, Hilda Nyambe-Silavwe, Gary Williamson
We have optimised the assays used to measure inhibition of rat and human α-glucosidases (sucrase and maltase activities), intestinal enzymes which catalyse the final steps of carbohydrate digestion. Cell-free extracts from fully differentiated intestinal Caco-2/TC7 monolayers were shown to be a suitable source of sucrase-isomaltase, with the same sequence as human small intestine, and were compared to a rat intestinal extract. The kinetic conditions of the assay were optimised, including comparison of enzymatic and chromatographic methods to detect the monosaccharide products...
September 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28911654/extraction-optimization-of-gallic-acid-catechin-%C3%A2-procyanidin-b2-epicatechin-epigallocatechin-gallate-and-epicatechin-gallate-their-simultaneous-identification-and-quantification-in-saraca-asoca
#5
Tushar Dhanani, Raghuraj Singh, Satyanshu Kumar
The objective of the present investigation was to optimize extraction conditions for maximum recovery of bioactive phenolics from different parts of Saraca asoca. Extraction recovery was optimized using a mixture of methanol and water in different proportions. For identification and quantification of six analytes, a rapid reversed phase ultra-performance liquid chromatography (UPLC) photo diode array detection method was developed. UPLC separation was achieved in a gradient elution mode on a C18 column with acetonitrile and aqueous phosphoric acid (0...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911541/food-macromolecule-based-nanodelivery-systems-for-enhancing-the-bioavailability-of-polyphenols
#6
REVIEW
Bing Hu, Xixia Liu, Chunlan Zhang, Xiaoxiong Zeng
Diet polyphenols-primarily categorized into flavonoids (e.g., flavonols, flavones, flavan-3-ols, anthocyanidins, flavanones, and isoflavones) and nonflavonoids (with major subclasses of stilbenes and phenolic acids)-are reported to have health-promoting effects, such as antioxidant, antiinflammatory, anticarcinoma, antimicrobial, antiviral, and cardioprotective properties. However, their applications in functional foods or medicine are limited because of their inefficient systemic delivery and poor oral bioavailability...
January 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28904061/a-randomized-controlled-trial-of-green-tea-extract-supplementation-and-mammographic-density-in-postmenopausal-women-at-increased-risk-of-breast-cancer
#7
Hamed Samavat, Giske Ursin, Tim H Emory, Eunjung Lee, Renwei Wang, Carolyn J Torkelson, Allison M Dostal, Karen Swenson, Chap T Le, Chung S Yang, Mimi C Yu, Douglas Yee, Anna H Wu, Jian-Min Yuan, Mindy S Kurzer
Epidemiological and animal studies suggest a protective role of green tea against breast cancer. However the underlying mechanism is not understood. We conducted a randomized, double-blinded, placebo-controlled phase II clinical trial to investigate whether supplementation with green tea extract (GTE) modifies mammographic density (MD), as a potential mechanism, involving 1,075 healthy postmenopausal women. Women assigned to the treatment arm consumed daily 4 decaffeinated GTE capsules containing 1315 mg total catechins including 843 mg epigallocatechin-3-gallate (EGCG) for 12 months...
September 13, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28901409/epigallocatechin%C3%A2-3%C3%A2-gallate-protects-from-high-glucose-induced-podocyte-apoptosis-via-suppressing-endoplasmic-reticulum-stress
#8
Chunhong Xiang, Xiaoyan Xiao, Bei Jiang, Mengkun Zhou, Yidan Zhang, Hui Li, Zhao Hu
Podocytes serve a critical role in the development of many glomerular diseases, including diabetic nephropathy (DN). Epigallocatechin‑3‑gallate (EGCG), a predominant polyphenolic component of green tea, has indicated its therapeutic effects in diabetes. In the present study, mouse podocyte cells were cultured in vitro, cell injury was induced by high glucose, and the protective effect of EGCG on cell proliferation and apoptosis and the underlying mechanisms were investigated. The results demonstrated that high glucose significantly inhibited cell proliferation after 48 and 72 h compared with normal glucose and mannitol treatment...
August 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899730/a-green-tea-polyphenol-epigallocatechin-3-gallate-enhances-neuroregeneration-after-spinal-cord-injury-by-altering-levels-of-inflammatory-cytokines
#9
Lucia Machova Urdzikova, Jiri Ruzicka, Kristyna Karova, Anna Kloudova, Barbora Svobodova, Anubhav Amin, Jana Dubisova, Meic Schmidt, Sarka Kubinova, Meena Jhanwar-Uniyal, Pavla Jendelova
Spinal cord injury (SCI) is a debilitating condition which is characterized by an extended secondary injury due to the presence of inflammatory local milieu. Epigallocatechin gallate (EGCG) appears to possess strong neuroprotective properties. Here, we evaluated the beneficial effect of EGCG on recovery from SCI. Male Wistar rats were given either EGCG or saline directly to the injured spinal cord and thereafter a daily IP injection. Behavior recovery was monitored by BBB, plantar, rotarod and flat-beam tests...
September 9, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28899506/green-tea-effects-on-cognition-mood-and-human-brain-function-a-systematic-review
#10
REVIEW
Edele Mancini, Christoph Beglinger, Jürgen Drewe, Davide Zanchi, Undine E Lang, Stefan Borgwardt
BACKGROUND: Green tea (Camellia sinensis) is a beverage consumed for thousands of years. Numerous claims about the benefits of its consumption were stated and investigated. As green tea is experiencing a surge in popularity in Western culture and as millions of people all over the world drink it every day, it is relevant to understand its effects on the human brain. PURPOSE: To assess the current state of knowledge in the literature regarding the effects of green tea or green tea extracts, l-theanine and epigallocatechin gallate both components of green tea-on general neuropsychology, on the sub-category cognition and on brain functions in humans...
October 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28894753/evaluation-of-epigallocatechin-3-gallate-modified-collagen-membrane-and-concerns-on-schwann-cells
#11
Chenyu Chu, Jia Deng, Cong Cao, Yi Man, Yili Qu
Collagen is an essential component of the extracellular matrix (ECM) and is a suitable material for nerve repair during tissue remodeling for fracture repair. Epigallocatechin-3-gallate (EGCG), an extract of green tea, shows various biological activities that are beneficial to nerve repair. Here, we developed modified collagen containing different concentrations of EGCG (0.0064%, 0.064%, and 0.64%, resp.) to induce Schwann cell proliferation and differentiation. Cell Counting Kit-8 test, live/dead assay, and SEM showed that collagen cross-linked by EGCG induced Schwann cell proliferation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28892583/fluorescent-probe-dcvj-shows-high-sensitivity-for-characterization-of-amyloid-beta-peptide-early-in-the-lag-phase
#12
Sureshbabu Nagarajan, Lisa Jill Lapidus
The aggregation of intrinsically disordered and misfolded proteins in the form of oligomers and fibrils plays a crucial role in a number of neuro and non-neurodegenerative diseases. Currently, most probes and biophysical techniques that detect and characterize fibrils to high resolution fail to show sensitivity and binding for oligomers. Here we show 9-(dicyano-vinyl) julolidine (DCVJ), a class of molecular rotor, binds Aβ early aggregates and reports on the kinetics as well as packing of oligomer formation...
September 11, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28891114/blood-brain-barrier-permeability-of-green-tea-catechin-metabolites-and-their-neuritogenic-activity-in-human-neuroblastoma-sh-sy5y-cells
#13
Keiko Unno, Monira Pervin, Aimi Nakagawa, Kazuaki Iguchi, Aya Hara, Akiko Takagaki, Fumio Nanjo, Akira Minami, Yoriyuki Nakamura
SCOPE: To understand the mechanism by which green tea lowers the risk of dementia, focus was placed on the metabolites of epigallocatechin gallate (EGCG), the most abundant catechin in green tea. Much of orally ingested EGCG is hydrolyzed to epigallocatechin (EGC) and gallic acid. In rats, EGC is then metabolized mainly to 5-(3', 5'-dihydroxyphenyl)-γ-valerolactone (EGC-M5) and its conjugated forms, which are distributed to various tissues. Therefore, we examined the permeability of these metabolites into the blood-brain barrier (BBB), and nerve cell proliferation/differentiation in vitro...
September 11, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28889069/electrochemical-assay-for-continuous-monitoring-of-dynamic-dna-methylation-process
#14
Li Zhang, Xiaofen Xiao, Yuzhi Xu, Danping Chen, Jun Chen, Yingjun Ma, Zong Dai, Xiaoyong Zou
A simple electrochemical strategy is reported for continuous monitoring of dynamic DNA methylation process over time. An electrochemical sensor was prepared by co-assembling of DNA probe and 6-mercapto-1-hexanol onto a gold electrode. The top of the DNA probe was labeled with 6-ferrocenylhexanethiol modified gold nanoparticle. The charge density between the C•G base pair was verified to be slightly reduced by DNA methylation, and could be further decelerated by ~ 25% upon co-locating a Br group onto methylated cytosine (mC)...
September 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28886220/anti-inflammatory-effects-of-polyphenols-in-arthritis
#15
REVIEW
Francesca Oliviero, Anna Scanu, Yessica Zamudio-Cuevas, Leonardo Punzi, Paolo Spinella
Polyphenols have been extensively investigated with regard to their anti-oxidant, anti-inflammatory, and immunomodulant properties in many inflammatory chronic conditions. The aim of this review is to summarize how these compounds can modulate the inflammatory pathways which characterize the most prevalent arthropathies including osteoarthritis, rheumatoid arthritis and crystal-induced arthritis. Among polyphenols, epigallocatechin gallate, carnosol, hydroxytyrosol, curcumin, resveratrol, kaempferol and genistein have been the most widely investigated in arthritis...
September 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28884125/green-tea-extracts-epigallocatechin-3-gallate-for-different-treatments
#16
REVIEW
Chenyu Chu, Jia Deng, Yi Man, Yili Qu
Epigallocatechin-3-gallate (EGCG), a component extracted from green tea, has been proved to have multiple effects on human pathological and physiological processes, and its mechanisms are discrepant in cancer, vascularity, bone regeneration, and nervous system. Although there are multiple benefits associated with EGCG, more and more challenges are still needed to get through. For example, EGCG shows low bioactivity via oral administration. This review focuses on effects of EGCG, including anti-cancer, antioxidant, anti-inflammatory, anticollagenase, and antifibrosis effects, to express the potential of EGCG and necessity of further studies in this field...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28879218/identification-of-epigallocatechin-3-o-3-o-methyl-gallate-egcg3-me-and-amino-acid-profiles-in-various-tea-camellia-sinensis-l-cultivars
#17
Hyang-Gi Ji, Yeong-Ran Lee, Min-Seuk Lee, Kyeng Hwan Hwang, Eun-Hee Kim, Jun Seong Park, Young-Shick Hong
This article includes experimental data on the identification of epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG3''Me) by 2-dimensional (2D) proton ((1)H) NMR analysis and on the information of amino acid and catechin compound profiles by HPLC analysis in leaf extracts of various tea cultivars. These data are related to the research article "Metabolic phenotyping of various tea (Camellia sinensis L.) cultivars and understanding of their intrinsic metabolism" (Ji et al., 2017) [1]. The assignment for EGCG3x''Me by (1)H NMR analysis was also confirmed with spiking experiment of its pure chemical...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28876921/binding-of-gallic-acid-and-epigallocatechin-gallate-to-heat-unfolded-whey-proteins-at-neutral-ph-alters-radical-scavenging-activity-of-in-vitro-protein-digests
#18
Yanyun Cao, Youling L Xiong
Preheated (80 °C for 9 min) whey protein isolate (HWPI) was reacted with 20, 120, and 240 μmol/g (protein basis) gallic acid (GA) or epigallocatechin gallate (EGCG) at neutral pH and 25 °C. Isothermal titration calorimetry and fluorometry showed a similar trend that GA binding to HWPI was moderate but weaker than EGCG binding. However, the shift of maximal fluorescence emission wavelength in opposite directions in response to GA (blue) and EGCG (red) suggests discrepant binding patterns. Electrophoresis results showed that EGCG induced formation of HWPI complexes while GA only had a marginal effect...
September 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28872903/in-vitro-biological-effects-of-sulforaphane-sfn-epigallocatechin-3-gallate-egcg-and-curcumin-on-breast-cancer-cells-a-systematic-review-of-the-literature
#19
Vincenza Gianfredi, Daniele Nucci, Samuele Vannini, Milena Villarini, Massimo Moretti
Much of the recent research in neoplasia has been focusing on the epigenetics of cancer cells, particularly as regards the search for potential molecular biomarkers that could be used for early diagnosis, effective treatment, and prognosis of several types of cancer. Carcinogenesis often starts with mutations in oncogenes and tumor suppressor genes, and it leads to anomalies in cellular processes as vital as cell cycle regulation and apoptosis. Because malignant changes arise as a result of genetic as well as epigenetic mechanisms, one possible means of intervention involves reprogramming gene expression, so as to-at least in part-revert the molecular alterations...
September 5, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28871467/epigallocatechin-gallate-protects-against-homocysteine-induced-vascular-smooth-muscle-cell-proliferation
#20
Xiao Li Zhan, Xiu Hong Yang, Yan Hong Gu, Li Li Guo, Hui Min Jin
Epigallocatechin gallate (EGCG), a bioactive ingredient of green tea, plays a protective role in the cardiovascular system. Homocysteine (Hcy) is a major risk factor for chronic kidney disease and cardiovascular disease. The present study aimed to investigate the role of EGCG in Hcy-induced proliferation of vascular smooth muscle cells (VSMCs) and its underlying mechanism. We also explored the roles of rennin-angiotensin system (RAS), extracellular signal-regulated kinases (ERK1/2), and p38 mitogen-activated protein kinase (p38 MAPK) in this process...
September 4, 2017: Molecular and Cellular Biochemistry
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