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Camellia sinensis

Shuyun Wu, Minghua Lai, Jiahao Luo, Jingwen Pan, Li-Ming Zhang, Liqun Yang
To understand the mechanism responsible for the α-amylase inhibitory activity of tea polysaccharides, the interaction between α-amylase and an acidic branched tea polysaccharide (TPSA) was investigated using fluorescence spectroscopy and resonance light scattering analysis. TPSA, exhibiting inhibitory activity towards α-amylase (the maximum inhibition percentage of 65%), was isolated from green tea (Camellia sinensis) and characterized by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, and gas chromatography...
October 15, 2016: International Journal of Biological Macromolecules
Junting Pan, Weidong Wang, Dongqin Li, Zaifa Shu, Xiaoli Ye, Pinpin Chang, Yuhua Wang
BACKGROUND: Nitric oxide (NO) functions as a critical signaling molecule in the low-temperature stress responses in plants, including polarized pollen tube growth in Camellia sinensis. Despite this, the potential mechanisms underlying the participation of NO in pollen tube responses to low temperature remain unclear. Here, we investigate alterations to gene expression in C. sinensis pollen tubes exposed to low-temperature stress and NO using RNA-Seq technology, in order to find the potential candidate genes related to the regulation of pollen tube elongation by NO under low-temperature stress...
October 18, 2016: BMC Genomics
Ilaria Peluso, Mauro Serafini
Consumption of Tea (Camellia Sinensis) has been correlated with low incidence of chronic pathologies, such as cardiovascular diseases and cancers, in which oxidative stress is deeply involved. Tea catechins and theaflavins are, respectively, the bioactive phytochemicals responsible for the antioxidant activity of Green Tea (GT) and Black Tea (BT). Apart from the redox properties, tea catechins and theaflavins could have also pharmacological activities, such as lowering effects on glucose, lipid and uric acid (UA)...
October 16, 2016: British Journal of Pharmacology
Tandeka Magcwebeba, Pieter Swart, Sonja Swanevelder, Elizabeth Joubert, Wentzel Gelderblom
Ultraviolet B (UVB) radiation is one of the major predisposing risk factors of skin cancer. The anticancer and photoprotective effects of unoxidized rooibos (Aspalathus linearis) and honeybush (Cyclopia) herbal teas, containing high levels of dihydrochalones and xanthones, respectively, have been demonstrated in skin cancer models in vivo. In the current study, the anti-inflammatory effects of methanol and aqueous extracts of these herbal teas were investigated in a UVB/HaCaT keratinocyte model with intracellular interleukin-1α (icIL-1α) accumulation as a biomarker...
October 2, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Tandeka Unathi Magcwebeba, Sylvia Riedel, Sonja Swanevelder, Pieter Swart, Dalene De Beer, Elizabeth Joubert, Wentzel Christoffel Andreas Gelderblom
OBJECTIVES: The relationship between polyphenol constituents, antioxidant properties of aqueous and methanol extracts of green tea (Camellia sinensis), the herbal teas, rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.), against skin cell viability was investigated in vitro. METHODS: The effect of extracts, characterised in terms of polyphenol content and antioxidant properties, on cell viability of premalignant, normal and malignant skin cells was determined...
September 27, 2016: Journal of Pharmacy and Pharmacology
Carola Biancalani, Matteo Cerboneschi, Francesco Tadini-Buoninsegni, Margherita Campo, Arianna Scardigli, Annalisa Romani, Stefania Tegli
Protection of plants against bacterial diseases still mainly relies on the use of chemical pesticides, which in Europe correspond essentially to copper-based compounds. However, recently plant diseases control is oriented towards a rational use of molecules and extracts, generally with natural origin, with lower intrinsic toxicity and a reduced negative environmental impact. In this work, polyphenolic extracts from vegetable no food/feed residues of typical Mediterranean crops, as Olea europaea, Cynara scolymus, and Vitis vinifera were obtained and their inhibitory activity on the Type Three Secretion System (TTSS) and the Quorum Sensing (QS) of the Gram-negative phytopathogenic bacterium Pseudomonas savastanoi pv...
2016: PloS One
Sadaf Lebaschi, Malak Hekmati, Hojat Veisi
The present study was conducted to synthesize palladium nanoparticles (Pd NPs) through a facile and green route using non-toxic and renewable natural black tea leaves (Camellia sinensis) extract, as the reducing and stabilizing agent. The as-prepared Pd@B.tea NPs catalyst was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), fourier transformed infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS)...
January 1, 2017: Journal of Colloid and Interface Science
Jackson M Muema, Joel L Bargul, Steven G Nyanjom, James M Mutunga, Sospeter N Njeru
BACKGROUND: Anopheles arabiensis and A. gambiae (sensu stricto) are the most prolific Afrotropical malaria vectors. Population control efforts of these two vectors have been hampered by extremely diverse larval breeding sites and widespread resistance to currently available insecticides. Control of mosquito larval stages using bioactive compounds of plant origin has the potential to suppress vector populations leading to concomitant reduction in disease transmission rates. In this study, we evaluated the efficacy of Camellia sinensis crude leaf extract and its fraction against the larvae of A...
2016: Parasites & Vectors
Tânia R Dias, Marco G Alves, Luís Rato, Susana Casal, Branca M Silva, Pedro F Oliveira
Prediabetes has been associated with alterations in male reproductive tract, especially in testis and epididymis. Moreover, in vitro studies described a promising action of tea (Camellia sinensis L.) against metabolic dysfunctions. Herein, we hypothesized that white tea (WTEA) ingestion by prediabetic animals could ameliorate the metabolic alterations induced by the disease in testicular and epididymal tissues, preserving sperm quality. WTEA infusion was prepared and its phytochemical profile was evaluated by (1)H-NMR...
August 26, 2016: Journal of Nutritional Biochemistry
Mateus Dassie Maximino, Cibely Silva Martin, Fernando Vieira Paulovich, Priscila Alessio
Polyphenols have attracted attention due to their antioxidant capacity and beneficial effects to health. Therefore, fast, inexpensive, and efficient methods to discriminate and to quantify polyphenols are of interest for food industry. In this paper, Layer-by-Layer films of poly(allylamine hydrochloride) and iron tetrasulfonated phthalocyanine were employed as sensor for determination of polyphenols in green tea (camellia sinensis), and green and roasted mate teas (ilex paraguariensis). The polyphenol sensor was tested in catechol standard solution by differential pulse voltammetry (DPV), reaching a limit of detection of 1...
October 2016: Journal of Food Science
Maria Ofitserova, Sareeta Nerkar
An HPLC method with postcolumn derivatization was developed and validated for the determination of theanine content in tea dietary ingredients and supplements. A variety of common commercially available supplement forms such as powders, liquid tinctures, tablets, softgels, and gelcaps, as well as three National Institute of Standards and Technology Camellia sinensis Standard Reference Materials were investigated in the study. A simple extraction procedure using citrate buffer at pH 2.2 allowed for the analysis of theanine without additional cleanup or concentration steps, even at low ppm levels...
September 15, 2016: Journal of AOAC International
Chengying Ma, Junxi Cao, Jianke Li, Bo Zhou, Jinchi Tang, Aiqing Miao
Leaf colour variation is observed in several plants. We obtained two types of branches with yellow and variegated leaves from Camellia sinensis. To reveal the mechanisms that underlie the leaf colour variations, combined morphological, histological, ionomic and proteomic analyses were performed using leaves from abnormal branches (variants) and normal branches (CKs). The measurement of the CIE-Lab coordinates showed that the brightness and yellowness of the variants were more intense than the CKs. When chloroplast profiles were analysed, HY1 (branch with yellow leaves) and HY2 (branch with variegated leaves) displayed abnormal chloroplast structures and a reduced number and size compared with the CKs, indicating that the abnormal chloroplast development might be tightly linked to the leaf colour variations...
2016: Scientific Reports
Xin Li, Golam J Ahammed, Zhi-Xin Li, Lan Zhang, Ji-Peng Wei, Chen Shen, Peng Yan, Li-Ping Zhang, Wen-Yan Han
Summer grown green tea is less popular due to bitterness and high astringency, which are attributed to high levels of tea polyphenols (TP) and low levels of amino acids (AA) in tea leaves (Camellia sinensis L.). Brassinosteroids (BRs), a group of steroidal plant hormones can regulate primary and secondary metabolism in a range of plant species under both normal and stress conditions. However, specific effects of BRs on the photosynthesis of tea plants and the quality of summer green tea are largely unknown...
2016: Frontiers in Plant Science
Moses C Wambulwa, Muditha K Meegahakumbura, Samson Kamunya, Alice Muchugi, Michael Möller, Jie Liu, Jian-Chu Xu, Sailesh Ranjitkar, De-Zhu Li, Lian-Ming Gao
Africa is one of the key centers of global tea production. Understanding the genetic diversity and relationships of cultivars of African tea is important for future targeted breeding efforts for new crop cultivars, specialty tea processing, and to guide germplasm conservation efforts. Despite the economic importance of tea in Africa, no research work has been done so far on its genetic diversity at a continental scale. Twenty-three nSSRs and three plastid DNA regions were used to investigate the genetic diversity, relationships, and breeding patterns of tea accessions collected from eight countries of Africa...
2016: Frontiers in Plant Science
Apinun Kanpiengjai, Naradorn Chui-Chai, Siriporn Chaikaew, Chartchai Khanongnuch
Miang is a fermented food product prepared from the tea leaves of Camellia sinensis var. assamica, and is traditionally produced in mountainous areas of northern Thailand. Although Miang has a long history and reveals deep-rooted cultural involvement with local people in northern Thailand, little is known regarding its microbial diversity. Yeasts were isolated from 47 Miang samples collected from 28 sampling sites, including eight provinces in upper northern Thailand. A hundred and seven yeast isolates were recovered and identified within 14 species based on the comparison of the D1/D2 sequence of the large subunit (LSU) rRNA gene...
December 5, 2016: International Journal of Food Microbiology
Chun-Fang Li, Yan-Xia Xu, Jian-Qiang Ma, Ji-Qiang Jin, Dan-Juan Huang, Ming-Zhe Yao, Chun-Lei Ma, Liang Chen
BACKGROUND: The new shoots of the albino tea cultivar 'Anji Baicha' are yellow or white at low temperatures and turn green as the environmental temperatures increase during the early spring. 'Anji Baicha' metabolite profiles exhibit considerable variability over three color and developmental stages, especially regarding the carotenoid, chlorophyll, and theanine concentrations. Previous studies focused on physiological characteristics, gene expression differences, and variations in metabolite abundances in albino tea plant leaves at specific growth stages...
2016: BMC Plant Biology
Roobakkumar Amsalingam, Prabu Gajjeraman, Nisha Sam, Vattakandy Jasin Rahman, Babu Azariah
Red spider mite (RSM), Oligonychus coffeae (Nietner) (Acarina: Tetranychidae), has gained special attention in view of their widespread occurrence as a pest on tea [Camellia sinensis L. (O. Kuntze)]. The development of acaricide (fenpropathrin) resistance has been screened in field populations (FPs) of RSMs from different tea-growing regions of south India and compared with a laboratory-susceptible population (SP) based on toxicity bioassay, detoxifying enzyme activities, analysis of acetylcholine esterase gene (AChE, 2064 bp), and their expression pattern using semiquantitative RT-PCR...
September 7, 2016: Applied Biochemistry and Biotechnology
Shannon L Glisan, Kimberly A Grove, Neela H Yennawar, Joshua D Lambert
Few studies have examined the effect of black tea (Camellia sinensis) theaflavins on obesity-related targets. Pancreatic lipase (PL) plays a central role in fat metabolism and is a validated target for weight loss. We compared the inhibitory efficacy of individual theaflavins and explored the underlying mechanism. Theaflavin-3,3'-digallate (TFdiG), theaflavin-3'-gallate, theaflavin-3-gallate, and theaflavin inhibited PL with IC50 of 1.9, 4.2, 3.0, and >10μmol/L. The presence and location of the galloyl ester moiety were essential for inhibitory potency...
February 1, 2017: Food Chemistry
Yi-Fang Li, Shu-Hua Ouyang, Yi-Qun Chang, Ting-Mei Wang, Wei-Xi Li, Hai-Yan Tian, Hong Cao, Hiroshi Kurihara, Rong-Rong He
Camellia sinensis var. puanensis Kurihara (Puan tea) is a kind of ancient tea plant newly found in Jiangxipo and the surrounding areas of Puan County (Guizhou, China). People there always believe that drinking Puan tea is beneficial to the promotion of health and prevention of diseases. However, detailed information on its compositions has not been reported. Therefore, in this study, the varieties and contents of purine alkaloids and polyphenols in Puan tea were identified and determined by HPLC and UFLC-Q-TOF-MS/MS...
February 1, 2017: Food Chemistry
Xiaoyun Yi, Sha Qiao, Lifeng Ma, Jie Wang, Jianyun Ruan
Drinking teas containing high fluoride (F) imposes fluorosis risk. The soil F bioavailability is an important factor influencing its uptake and contents in teas. The present work was conducted to investigate F fractions in soil and their bioavailability to tea plants. Tea seedlings were cultivated on 6 typical soils treated with a mixture consisting of dolomite, lime, peat and KCl at variable rates in the pot experiment. Soils and young shoots were collected in pairs from 63 sites of 21 plantations in a field experiment...
September 3, 2016: Environmental Geochemistry and Health
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