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https://www.readbyqxmd.com/read/28439515/endogenous-retroviruses-with-us-and-against-us
#1
REVIEW
Thomas J Meyer, Jimi L Rosenkrantz, Lucia Carbone, Shawn L Chavez
Mammalian genomes are scattered with thousands of copies of endogenous retroviruses (ERVs), mobile genetic elements that are relics of ancient retroviral infections. After inserting copies into the germ line of a host, most ERVs accumulate mutations that prevent the normal assembly of infectious viral particles, becoming trapped in host genomes and unable to leave to infect other cells. While most copies of ERVs are inactive, some are transcribed and encode the proteins needed to generate new insertions at novel loci...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28417059/susceptibility-of-human-oral-squamous-cell-carcinoma-oscc-h103-and-h376-cell-lines-to-retroviral-oskm-mediated-reprogramming
#2
Nalini Devi Verusingam, Swee Keong Yeap, Huynh Ky, Ian C Paterson, Suan Phaik Khoo, Soon Keng Cheong, Alan H K Ong, Tunku Kamarul
Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity...
2017: PeerJ
https://www.readbyqxmd.com/read/28413006/generation-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-64year-old-male-patient-with-multiple-schwannoma
#3
Shaokun Zhang, Zhenshan Lv, Yang Hu, Lidi Liu, Weiquan Gong, Qiao Li, Hong Wu
Peripheral blood was collected from a clinically diagnosed 64-year old male multiple schwannoma patient. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413005/generation-of-a-nrf2-homozygous-knockout-human-embryonic-stem-cell-line-using-crispr-cas9
#4
So-Jung Kim, Omer Habib, Jin-Soo Kim, Hyo-Won Han, Soo Kyung Koo, Jung-Hyun Kim
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413004/derivation-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-79year-old-sporadic-male-parkinson-s-disease-patient
#5
Shaokun Zhang, Lidi Liu, Yang Hu, Zhenshan Lv, Qiao Li, Weiquan Gong, Hui Sha, Hong Wu
Peripheral blood was collected from a clinically diagnosed 79-year old male sporadic Parkinson's disease patient. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model can be used to study the mechanism of sporadic Parkinson's disease and to test new drugs...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413002/characterization-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-72year-old-male-patient-with-later-onset-alzheimer-s-disease
#6
Shaokun Zhang, Zhenshan Lv, Songyuan Zhang, Lidi Liu, Qiao Li, Weiquan Gong, Hui Sha, Hong Wu
Peripheral blood was collected from a clinically diagnosed 72-year old male patient with later onset Alzheimer's disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for studying the pathological mechanism of Alzheimer's disease...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413001/development-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-60year-old-female-patient-with-multiple-schwannoma
#7
Shaokun Zhang, Zhenshan Lv, Yan Liu, Qiao Li, Weiquan Gong, Lidi Liu, Hong Wu
Peripheral blood was collected from a clinically diagnosed 60-year old female patient with multiple schwannoma. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395806/generation-of-human-ipscs-from-an-essential-thrombocythemia-patient-carrying-a-v501l-mutation-in-the-mpl-gene
#8
Senquan Liu, Zhaohui Ye, Yongxing Gao, Chaoxia He, Donna W Williams, Alison Moliterno, Jerry Spivak, He Huang, Linzhao Cheng
Activating point mutations in the MPL gene encoding the thrombopoietin receptor are found in 3%-10% of essential thrombocythemia (ET) and myelofibrosis patients. Here, we report the derivation of induced pluripotent stem cells (iPSCs) from an ET patient with a heterozygous MPL V501L mutation. Peripheral blood CD34(+) progenitor cells were reprogrammed by transient plasmid expression of OCT4, SOX2, KLF4, c-MYC plus BCL2L1 (BCL-xL) genes. The derived line M494 carries a MPL V501L mutation, displays typical iPSC morphology and characteristics, are pluripotent and karyotypically normal...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395805/generation-of-a-human-induced-pluripotent-stem-cell-ipsc-line-carrying-the-parkinson-s-disease-linked-lrrk2-variant-s1647t
#9
Dongrui Ma, Shin Hui Ng, Li Zeng, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with S1647T variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will be useful for further function studies and therapeutic screening...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395804/development-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-parkinson-s-disease-patient-carrying-the-n551k-variant-in-lrrk2-gene
#10
Dongrui Ma, Ebonne Yulin Ng, Li Zeng, Christina Ying Yan Lim, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with N551K variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model can complement in vivo PD models for pathophysiological studies and drug screening...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395803/derivation-of-human-induced-pluripotent-stem-cell-ipsc-line-with-lrrk2-gene-r1398h-variant-in-parkinson-s-disease
#11
Dongrui Ma, Murni Tio, Shin Hui Ng, Li Zeng, Christina Ying Yan Lim, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 72-year old female Parkinson's disease (PD) patient with R1398H variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model provides a good platform for studying the mechanism of PD, and also for drug testing and gene therapy studies...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395802/reprogramming-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-parkinson-s-disease-patient-with-a-r1628p-variant-in-the-lrrk2-gene
#12
Dongrui Ma, Wei Zhou, Ebonne Yulin Ng, Li Zeng, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 59-year old male Parkinson's disease (PD) patient with R1628P variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will provide a good resource for further pathophysiological studies of PD...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395800/derivation-and-characterization-of-the-human-embryonic-stem-cell-line-cr-4-differentiation-to-human-retinal-pigment-epithelial-cells
#13
John L Mazzilli, Aleksey Y Domozhirov, Stacey L Mueller-Ortiz, Charles A Garcia, Rick A Wetsel, Eva M Zsigmond
The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395797/generation-and-characterization-of-human-ipsc-line-mml-6838-cl2-from-mobilized-peripheral-blood-derived-megakaryoblasts
#14
Marten Hansen, Eszter Varga, Tatjana Wüst, Nelleke Brouwer, Hugues Beauchemin, Clemens Mellink, Anne-Marie van der Kevie-Kersemaekers, Tarik Möröy, Bert van der Reijden, Marieke von Lindern, Emile van den Akker
Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395746/derivation-of-the-human-induced-pluripotent-stem-cell-line-mui017-a-from-a-patient-with-homozygous-hemoglobin-constant-spring
#15
Wasinee Wongkummool, Warun Maneepitasut, Thongperm Munkongdee, Pirut Tong-Ngam, Amornrat Tangprasittipap, Saovaros Svasti, Narisorn Kitiyanant, Kittiphong Paiboonsukwong, Suthat Fucharoen, Alisa Tubsuwan
Hemoglobin Constant Spring (HbCS, HBA2: c.427T>C) is a common nondeletional α-thalassemia resulting from a nucleotide substitution at the termination codon of the HBA2 gene. Homozygosity for HbCS is characterized with mild anemia, jaundice, and splenomegaly. In this study, the human induced pluripotent stem cell line MUi017-A was successfully generated from peripheral blood CD34+ hematopoietic progenitors of a 52year old female with homozygous HbCS. The MUi017-A cell line exhibited embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of differentiating into the three germ layers...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395745/establishment-of-mui009-a-human-induced-pluripotent-stem-cells-from-a-32year-old-male-with-homozygous-%C3%AE-%C3%A2-thalassemia-coinherited-with-heterozygous-%C3%AE-thalassemia-2
#16
Wasinee Wongkummool, Warun Maneepitasut, Pirut Tong-Ngam, Amornrat Tangprasittipap, Thongperm Munkongdee, Chanikarn Boonchuay, Saovaros Svasti, Narisorn Kitiyanant, Kittiphong Paiboonsukwong, Suthat Fucharoen, Alisa Tubsuwan
The thalassemias are a group of genetic disorders characterized by a deficiency in the synthesis of globin chains. In this study the MUi009-A human induced pluripotent stem cell line was successfully generated from peripheral blood CD34+ haematopoietic progenitors of a 32year old male who had coinherited a homozygous β°-thalassemia mutation at codon 41/42 (-TCTT) and a heterozygous α-thalassemia 4.2 deletion. The MUi009-A cell line exhibited embryonic stem cell characteristics with consistent pluripotency marker expression and the capability of differentiating into the three germ layers...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395738/a-feeder-and-xeno-free-human-induced-pluripotent-stem-cell-line-obtained-from-primary-human-dermal-fibroblasts-with-epigenetic-repression-of-reprogramming-factors-expression-gpcci001-a
#17
Michał Stefan Lach, Joanna P Wroblewska, Ewelina Augustyniak, Katarzyna Kulcenty, Wiktoria Maria Suchorska
The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator which regulates transgene expression was used. This method takes advantage of epigenetic regulation of transcription and allows for stable silencing of the reprogramming factors in obtained hiPS cells. To increase the potential utility of hiPSCs for clinical applications, they were adapted to feeder- and xeno-free conditions...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395736/generation-of-an-induced-pluripotent-stem-cell-line-from-a-loeys-dietz-syndrome-patient-with-transforming-growth-factor-beta-receptor-2-gene-mutation
#18
Kui Hu, Jun Li, Kai Zhu, Jinmiao Chen, Dingqian Liu, Yulin Wang, Yongxin Sun, Hao Lai, Chunsheng Wang
Loeys-Dietz syndrome (LDS) is an autosomal-dominant connective tissue disorder, commonly caused by genetic mutation of transforming growth factor-beta receptor (TGFBR)-1 or TGFBR2. This study describes the generation of human induced pluripotent stem cells (hiPSCs) from peripheral blood mononuclear cells obtained from an LDS patient with TGFBR2 mutation (R193W). Analysis confirmed the cells had a normal karyotype, expressed typical pluripotency markers, had the ability to differentiate into all three germ layers in vivo, and retained the TGFBR2 mutation from the derived hiPSCs...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28393187/generation-and-characterization-of-induced-pluripotent-stem-cells-from-guinea-pig-fetal-fibroblasts
#19
Yuehong Wu, Ouyang Li, Chengwen He, Yong Li, Min Li, Xiaoming Liu Liu, Yujiong Wang, Yulong He
Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28389990/high-mobility-group-box-1-regulates-cytoprotective-autophagy-in-a-mouse-spermatocyte-cell-line-gc-2spd-exposed-to-cadmium
#20
Z Ou, Y Chen, X Niu, W He, B Song, D Fan, X Sun
BACKGROUND: Cadmium (Cd) is an environmental and industrial pollutant that induces a broad spectrum of toxicological effects, influences a variety of human organs, and is associated with poor semen quality and male infertility. Increasing evidence demonstrates that Cd induces testicular germ cell apoptosis in rodent animals. However, the specific effect of Cd exposure on autophagy in germ cells is poorly understood. METHODS: We investigate the role of high-mobility group box 1 protein (HMGB1), a ubiquitous nuclear protein, on Cd-evoked autophagy in a mouse spermatocyte cell line (GC-2spd)...
April 7, 2017: Irish Journal of Medical Science
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