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TLR4 KIDNEY

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https://www.readbyqxmd.com/read/28743964/toll-like-receptor-4-deficiency-reduces-oxidative-stress-and-macrophage-mediated-inflammation-in-hypertensive-kidney
#1
Sathnur Pushpakumar, Lu Ren, Sourav Kundu, Alejandra Gamon, Suresh C Tyagi, Utpal Sen
Oxidative stress and inflammation are integral to hypertension-induced renal injury. A unifying feature for the two components is Toll-like receptors (TLR), which are key regulators of the innate immune system. Recent studies implicate TLR4 activation and oxidative stress in cardiovascular diseases and also as a link between inflammation and hypertension. However, its role in hypertension induced renal injury remains unexplored. In the present study, we investigated whether TLR-4 deficiency reduces Ang-II-induced renal injury and fibrosis by attenuating reactive oxygen species (ROS) production and inflammation...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28734896/porcine-cd14-gene-silencing-partially-inhibited-the-bacterial-immune-response-mediated-by-tlr4-signaling-pathway
#2
Chaohui Dai, Haifei Wang, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Cluster of differentiation antigen 14 (CD14) is the membrane receptor protein in Toll-like Receptor 4 (TLR4) signaling pathway, which plays an important regulation role in not only innate immune response but also adaptive immune response. In this study, the pig kidney epithelial cell (PK15) line with CD14 gene silencing mediated by lentivirus was established and cells of CD14-RNAi and NC group were exposed to three kinds of Escherichia coli (E. coli F18ab, E. coli F18ac and E. coli K88ac) and LPS. Then qPCR and western blot were used to detect expression levels of TLR4 signaling pathway-related genes...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28706136/swine-irf3-irf7-attenuates-inflammatory-responses-through-tlr4-signaling-pathway
#3
Pei-Ge Chen, Yan-Jing Guan, Guang-Ming Zha, Xian-Qin Jiao, He-Shui Zhu, Cheng-Yu Zhang, Yue-Ying Wang, He-Ping Li
To explore the role of IRF3/IRF7 during inflammatory responses, we investigated the effects of swine IRF3/IRF7 on TLR4 signaling pathway and inflammatory factors expression in porcine kidney epithelial PK15 cell lines. We successfully constructed eukaryotic vectors PB-IRF3 and PB-IRF7, transfected these vectors into PK15 cells and observed GFP under a fluorescence microscope. In addition, RT-PCR was also used to detect transfection efficiency. We found that IRF3/IRF7 was efficiently overexpressed in PK15 cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28685413/telluric-acid-ameliorates-endotoxemic-kidney-injury-in-mice-involvement-of-tlr4-nrf2-and-pi3k-akt-signaling-pathways
#4
Ahmed F Mohamed, Marwa M Safar, Hala F Zaki, Helmy M Sayed
Being one of the most abundant trace elements in the human body, the therapeutic potential of tellurium-based compounds has been a target of interest. Recent reports denoted their redox-modulating and anti-inflammatory activities in experimental endotoxemia. However, their potential nephroprotective effect against endotoxemic kidney injury is yet to be elucidated. This study investigated the possible renoprotective effect of telluric acid (TEL) against lipopolysaccharide (LPS)-induced acute kidney injury (AKI) in mice, targeting toll-like receptor 4 (TLR4), phosphoinositide 3-kinase (PI3K)/Akt, and nuclear factor-erythroid 2-related factor-2 (Nrf2) pathways as possible mechanistic contributors to TEL's effect...
July 6, 2017: Inflammation
https://www.readbyqxmd.com/read/28655852/role-of-east-6-in-renal-injury-by-regulating-microrna-155-expression-via-tlr4-myd88-signaling-pathway-in-mice-with-mycobacterium-tuberculosis-infection
#5
Zhong-Qi Zhou, Zhi-Kui Wang, Lei Zhang, Yue-Qin Ren, Zhong-Wei Ma, Nan Zhao, Fu-Yun Sun
The study aims to investigate the underlying mechanism involved with the early secretory antigenic target-6 (ESAT-6) in renal injury through regulation of the expression of microRNA-155 (miR-155) through the TLR4/MyD88 signaling pathway in Mycobacterium tuberculosis ( MTB )-infected mice. Sixty C57BL/6 mice with MTB-induced renal injury were randomly assigned into control, MTB, mimic, inhibitor and inhibitor + ESAT6 and inhibitor + ESAT6 + TAK242 groups. Body weight, the ratio of kidney weight to body weight (Kw/Bw), blood urea nitrogen (BUN) and serum creatinine (Scr) of mice were measured...
June 27, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28641285/role-of-glycyrrhizin-in-the-reduction-of-inflammation-in-diabetic-kidney-disease
#6
Vikram Thakur, Syeda Nargis, Mayra Gonzalez, Swetak Pradhan, Daniel Terreros, Munmun Chattopadhyay
BACKGROUND: Diabetic kidney disease (DKD) is one of the most debilitating complications of type 2 diabetes. Recent evidence suggests chronic inflammation to be one of the causal factors of DKD. The mechanisms entailed are not completely elucidated except that a variety of cytokines play a major role in this process. High mobility group box 1 (HMGB1) is a pro-inflammatory toll-like receptor-4 (TLR4)-binding cytokine that is involved in inflammation-associated gene expression. This investigation was designed to assess the involvement of HMGB1, TLR-4, and nuclear factor (NF)-κB in the development of DKD and to evaluate that whether blocking HMGB1 by its natural inhibitor Glycyrrhizin (GLC) can reduce the progression of the disease...
June 23, 2017: Nephron
https://www.readbyqxmd.com/read/28623542/identification-of-differentially-expressed-mirnas-associated-with-chronic-kidney-disease-mineral-bone-disorder
#7
Kyung Im Kim, Sohyun Jeong, Nayoung Han, Jung Mi Oh, Kook-Hwan Oh, In-Wha Kim
The purpose of this study is to characterize a meta-signature of differentially expressed mRNA in chronic kidney disease (CKD) to predict putative microRNA (miRNA) in CKD-mineral bone disorder (CKD-MBD) and confirm the changes in these genes and miRNA expression under uremic conditions by using a cell culture system. PubMed searches using MeSH terms and keywords related to CKD, uremia, and mRNA arrays were conducted. Through a computational analysis, a meta-signature that characterizes the significant intersection of differentially expressed mRNA and expected miRNAs associated with CKD-MBD was determined...
June 14, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28616865/isoacteoside-a-dihydroxyphenylethyl-glycoside-exhibits-anti-inflammatory-effects-through-blocking-toll-like-receptor-4-dimerization
#8
Hongwei Gao, Yankun Cui, Naixin Kang, Xin Liu, Yanli Liu, Yue Zou, Ziyu Zhang, Xiaoran Li, Shilin Yang, Ji Li, Chunming Wang, Qiong-Ming Xu, Xiuping Chen
BACKGROUND AND PURPOSE: Isoacteoside (is a phenylethanoid isolated from Monochasma savatieri Franch. ex Maxim., which is an anti-inflammatory herb widely used in traditional Chinese medicine. However, the exact mechanism of the anti-inflammatory activity of isoacteoside is not completely understood. In this study, its anti-inflammatory mechanism was elucidated in mouse macrophages. EXPERIMENTAL APPROACH: The expression of the NF-κB pathway, MAPK pathway, iNOS, TNF-α, IL-6 and IL-1β was evaluated using Western blotting, quantitative real-time PCR or ELISA...
June 14, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28600543/innate-and-adaptive-immunity-are-progressively-activated-in-parallel-with-renal-injury-in-the-5-6-renal-ablation-model
#9
Camilla Fanelli, Simone C A Arias, Flavia G Machado, Jessica K Okuma, Denise M A C Malheiros, Hatylas Azevedo, Carlos A Moreira-Filho, Niels O S Camara, Clarice K Fujihara, Roberto Zatz
The mechanisms triggering renal inflammation in chronic kidney disease (CKD) are unclear. We performed a detailed analysis of the time course of innate and adaptive immunity activation in the 5/6 renal ablation (Nx) model. Munich-Wistar rats undergoing Nx were studied 15, 60 and 120 days after ablation. Hypertension, albuminuria, creatinine retention, interstitial expansion and infiltration by macrophages and T-lymphocytes were already evident 15 days after Nx. PCR-array was used to screen for altered gene expression, whereas gene and protein expressions of TLR4, CASP1, IL-1β and NLRP3 were individually assessed...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28596381/a-dual-agonist-of-farnesoid-x-receptor-fxr-and-the-g-protein-coupled-receptor-tgr5-int-767-reverses-age-related-kidney-disease-in-mice
#10
Xiaoxin X Wang, Yuhuan Luo, Dong Wang, Luciano Adorini, Mark Pruzanski, Evgenia Dobrinskikh, Moshe Levi
Even in healthy individuals, renal function gradually declines during aging. However, an observed variation in the rate of this decline has raised the possibility of slowing or delaying age-related kidney disease. One of the most successful interventional measures that slows down and delays age-related kidney disease is caloric restriction. We undertook the present studies to search for potential factors that are regulated by caloric restriction and act as caloric restriction mimetics. Based on our prior studies with the bile acid-activated nuclear hormone receptor farnesoid X receptor (FXR) and G protein-coupled membrane receptor TGR5 that demonstrated beneficial effects of FXR and TGR5 activation in the kidney, we reasoned that FXR and TGR5 could be excellent candidates...
July 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28591009/surfactant-proteins-a-and-d-attenuate-lps-induced-apoptosis-in-primary-intestinal-epithelial-cells-iecs
#11
Linlin Zhang, Qinghe Meng, Natesh Yepuri, Guirong Wang, Xiuming Xi, Robert N Cooney
INTRODUCTION: SP-A/D KO mice with sepsis demonstrate more severe lung, kidney and gut injury/apoptosis than WT controls. We hypothesize SP-A and SP-D directly regulate LPS-induced P38 MAPK activation and gut apoptosis during sepsis. METHODS: Primary IECs were established from SP-A/D KO or C57BL/6 WT mice, stimulated with LPS and harvested at 24 h. IECs from WT mice were treated with SP-A, SP-D or vehicle for 20 h, then LPS for 24 h. Apoptosis, cleaved caspase-3 levels and the ratio of BAX/Bcl-2 were assayed...
June 6, 2017: Shock
https://www.readbyqxmd.com/read/28570013/toll-like-receptor-2-antagonists-identified-through-virtual-screening-and-experimental-validation
#12
Prasannavenkatesh Durai, Hyeon-Jun Shin, Asma Achek, Hyuk-Kwon Kwon, Rajiv Gandhi Govindaraj, Suresh Panneerselvam, Dhanusha Yesudhas, Jiwon Choi, Kyoung Tai No, Sangdun Choi
Toll-like receptor 2 (TLR2) antagonists are key therapeutic targets because they inhibit several inflammatory diseases caused by surplus TLR2 activation. In this study, we identified two novel nonpeptide TLR2 antagonists, C11 and C13, through pharmacophore-based virtual screening. At 10 μm, the level of interleukin (IL)-8 inhibition by C13 and C11 in human embryonic kidney TLR2 overexpressing cells was comparable to the commercially available TLR2 inhibitor CU-CPT22. In addition, C11 and C13 acted in mouse macrophage-like RAW 264...
June 1, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28562479/sepsis-up-regulates-cd14-expression-in-a-myd88-dependent-and-trif-independent-pathway
#13
Zhixia Chen, Zhenzhen Shao, Shuya Mei, Zhengzheng Yan, Xibing Ding, Timothy Billiar, Quan Li
An overwhelming immune response, particularly from macrophages, plays a critical role in survival and organ damage in sepsis patients. TLRs are important receptors to recognize the conserved motifs expressed by invading bacteria. The TLRs except TLR3 signal via a MyD88 dependent pathway. TLR3 uses a TRIF dependent pathway, while TLR4 uses both MyD88 and TRIF dependent pathways. Previous studies indicated that CD14 was necessary for TLRs dependent production of pro-inflammatory cytokines. Blocking CD14 protected against the deleterious systemic inflammatory response associated with sepsis...
May 29, 2017: Shock
https://www.readbyqxmd.com/read/28544543/acute-toll-like-receptor-4-activation-impairs-rat-renal-microvascular-autoregulatory-behaviour
#14
Justin P Van Beusecum, Shali Zhang, Anthony K Cook, Edward W Inscho
AIM: Little is known about how toll-like receptor 4 (TLR4) influences the renal microvasculature. We hypothesized that acute TLR4 stimulation with lipopolysaccharide (LPS) impairs afferent arteriole autoregulatory behaviour, partially through reactive oxygen species (ROS). METHODS: We assessed afferent arteriole autoregulatory behaviour after LPS treatment (1 mg kg(-1) ; i.p.) using the in vitro blood perfused juxtamedullary nephron preparation. Autoregulatory behaviour was assessed by measuring diameter responses to step-wise changes in renal perfusion pressure...
May 25, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28542370/upregulation-of-tlr4-via-pkc-activation-contributes-to-impaired-wound-healing-in-high-glucose-treated-kidney-proximal-tubular-cells
#15
Jianping Peng, Hang Zheng, Xia Wang, Zhixiang Cheng
Acute kidney injury (AKI) leads to a worse prognosis in diabetic patients compared with prognoses in non-diabetic patients, but whether and how diabetes affects kidney repair after AKI remains unknown. Here, we used scratch-wound healing and transwell migration models to examine whether and how wound healing is affected by high glucose levels in cultured kidney proximal tubular cells (RPTC). The results show that scratch-wound healing and transwell migration were significantly slower in high-glucose-treated kidney tubular cells (30 mM glucose) than in low-glucose-treated cells (5...
2017: PloS One
https://www.readbyqxmd.com/read/28536433/a-new-model-of-self-resolving-leptospirosis-in-mice-infected-with-a-strain-of-leptospira-interrogans-serovar-autumnalis-harboring-lps-signaling-only-through-tlr4
#16
Bili Xia, Le Sun, Xia Fan, Haihan Xiao, Yongzhang Zhu, Jinhong Qin, Chengsong Cai, Wei Zhao, Yung-Fu Chang, Yan Zhang, Xiaokui Guo, Ping He
Leptospirosis is an emerging worldwide zoonosis caused by pathogenic Leptospira spp. Our understanding of leptospirosis pathogenesis and host immune response remains limited, while mechanistic studies are hindered by a lack of proper animal models and immunological reagents. Here we established a murine model of acute and self-resolving leptospirosis by infecting 10-week-old C57BL/6 mice with Leptospira interrogans serovar Autumnalis strain 56606v, with characteristic manifestations including jaundice as well as subcutaneous and pulmonary bleeding, but no kidney lesions...
May 24, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28485782/nac-attenuates-adriamycin-induced-nephrotic-syndrome-in-rats-through-regulating-tlr4-signaling-pathway
#17
H Yao, Z-Y Cai, Z-X Sheng
OBJECTIVE: Nephrotic syndrome (NS) is a detrimental renal disease that affects a large population. It is suggested that Toll-like Receptor 4 (TLR4) signaling pathway plays an important role in NS. The aim of this study was to evaluate the immunosuppressive effect of N-acetylcysteine (NAC) in the treatment of NS elucidate its interaction with TLR4 pathway in a rat model. MATERIALS AND METHODS: Rat NS model was constructed using the Bertain method by injecting adriamycin (4...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28485325/lipopolysaccharide-stimulates-surfactant-protein-a-in-human-renal-epithelial-hk-2-cells-through-upregulating-toll-like-receptor-4-dependent-mek1-2-erk1-2-nf-%C3%AE%C2%BAb-pathway
#18
Jiao Liu, Guang Li, Wen-Jie Xie, Lu Wang, Rui Zhang, Ke-Sheng Huang, Qing-Shan Zhou, De-Chang Chen
BACKGROUND: Surfactant protein-A (SP-A) contributes to the regulation of sepsis-induced acute kidney injury. In a previous study, we demonstrated that the expression of SP-A in the human renal tubular epithelial (HK-2) cells can be stimulated by lipopolysaccharide (LPS). The present study evaluated the possible signal-transducing mechanisms of LPS-induced SP-A biosynthesis in the HK-2 cells. METHODS: Tetrazolium salt colorimetry (MTT) assay was used to detect cell viability of HK-2 cells after LPS stimulation on different time points...
May 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28472797/up-regulation-of-hmgb1-exacerbates-renal-ischemia-reperfusion-injury-by-stimulating-inflammatory-and-immune-responses-through-the-tlr4-signaling-pathway-in-mice
#19
Chuan-Bao Chen, Long-Shan Liu, Jian Zhou, Xiao-Ping Wang, Ming Han, Xing-Yuan Jiao, Xiao-Shun He, Xiao-Peng Yuan
BACKGROUND/AIMS: The aim of this study was to elucidate how high-mobility group box 1 (HMGB1) exacerbates renal ischemic-reperfusion injury (IRI) by inflammatory and immune responses through the toll-like receptor 4 (TLR4) signaling pathway. METHODS: A total of 30 wild-type (WT) mice and 30 TLR4 knockout (TLR4-/-) mice were selected and then randomly assigned to the Sham, I/R or HMGB1 groups. The serum and kidney tissues of all mice were collected 24 h after the perfusion...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28466227/protective-effects-and-mechanisms-of-shenhua-tablet-on-toll-like-receptors-in-rat-model-of-renal-ischemia-reperfusion-injury
#20
Qing-Ping Li, Ri-Bao Wei, Xi Yang, Xiao-Yong Zheng, Ting-Yu Su, Meng-Jie Huang, Zhong Yin, Xiang-Mei Chen
OBJECTIVES: To investigate the protective effects and potential mechanisms of Shenhua Tablet (, SHT) on the toll-like receptors (TLRs)-mediated signaling pathways in a rat model of kidney ischemia-reperfusion injury (IRI). METHODS: Sixty male Wistar rats were randomly divided into 5 groups: sham surgery, model control, astragaloside (150 mg•kg(-1)•d(-1)), low- and high-dose SHT (1.5 and 3.0 g•kg(-1)•d(-1), repectively) groups. One week after drug treatment, rats underwent surgery to establish the IRI models...
May 3, 2017: Chinese Journal of Integrative Medicine
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