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Akira Tanimura, Risen Hirai, Miki Nakamura, Masataka Takeshita, Shotaro Hagiwara, Akiyoshi Miwa
Two regimens are commonly used for peripheral blood hematopoietic stem cell harvesting (PBSCH) in multiple myeloma: high-dose cyclophosphamide (HD-CY) + granulocyte-colony stimulating factor (G-CSF), and G-CSF alone. The objective of the present study was to evaluate the anti-myeloma effect of the PBSCH regimen including HD-CY. We retrospectively assessed harvesting efficiency, complications, and anti-myeloma effects in 115 patients receiving HD-CY + G-CSF (HD-CY group) and 32 patients receiving G-CSF alone (G-alone group)...
May 2018: International Journal of Hematology
Hiroaki Tanaka, Akihiro Ishii, Yasumasa Sugita, Ryo Shimizu, Fumi Sato, Yukie Sakuma, Rie Iwai, Shinichiro Kakuta
For optimizing CD34+ cell collection, appropriately timing peripheral blood stem cell harvest (PBSCH) initiation is crucial. Automatic cell analyzers with the immature myeloid information channel provide hematopoietic progenitor cell (HPC) count, a surrogate marker of CD34+ cells, which can be obtained within a few minutes without requiring monoclonal antibodies. The final decision on PBSCH initiation can be made using the HPC count obtained on the morning of the harvest day. Herein, we evaluated the impact of the HPC count as an indicator for the optimal timing of PBSCH in clinical practice over 9 years...
2017: Journal of Clinical and Experimental Hematopathology: JCEH
Toby A Eyre, Andrew J King, Andrew Peniket, Vanderson Rocha, Graham P Collins, Rachel Pawson
BACKGROUND: Rarely, healthy donors fail adequate peripheral blood stem cell (PBSC) mobilization. If the recipient has already received conditioning chemotherapy, this can result in the donor undergoing urgent marrow harvest under general anesthesia. Plerixafor is a novel CXCR4 antagonist, licensed for autologous PBSC harvest (PBSCH) in patients who failed mobilization with granulocyte-colony-stimulating factor (G-CSF) alone. Experience using plerixafor in healthy allogeneic donors failing G-CSF mobilization is scarce...
May 2014: Transfusion
Takahiko Nakane, Hirohisa Nakamae, Hideo Koh, Mika Nakamae, Ran Aimoto, Yoshiki Terada, Ki-Ryang Koh, Takahisa Yamane, Masayuki Hino
Side effects of varying severity are frequent in peripheral blood stem cell harvest (PBSCH). Life-threatening complications associated with PBSCH have also been reported. Heart rate variability (HRV), which reflects sympathovagal balance and autonomic cardiovascular control, has been a subject of intense interest in various diseases precipitating sudden death. Here, we prospectively assessed the impact of leukapheresis on HRV among autologous hematopoietic cell transplant patients and healthy donors. We found that HRV indicators, the standard deviation of normal-to-normal intervals (SDNN) value, the square root of the mean of the sum of squared differences between the adjacent normal-to-normal interval (r-MSSD) value, total frequency (TF), high frequency (HF) and low frequency (LF) powers decreased significantly to morbid levels during leukapheresis (all P < 0...
April 2010: International Journal of Hematology
Noriko Namba, Keitaro Matsuo, Shiro Kubonishi, Tomoko Kikuchi, Yoshinobu Maeda, Masami Niiya, Katsuji Shinagawa, Norio Koide, Kazuma Ikeda, Mitsune Tanimoto
BACKGROUND: Allogeneic peripheral blood stem cell (PBSC) transplantation is widely performed as a curative therapy for hematopoietic malignancies. Donors for PBSC harvest (PBSCH) are usually healthy subjects and undergo granulocyte-colony-stimulating factor treatment and apheresis procedures. A considerable proportion of donors experience poor mobilization, necessitating additional harvesting or marrow collection or remobilization. Although some characteristics have been reported to correlate with poor mobilization, they may not be taken into account in selecting PBSC donors...
November 2009: Transfusion
Ljiljana Puskar, Perdita E Barran, Bridgette J Duncombe, Daniel Chapman, Anthony J Stace
Using a pickup technique in association with high-energy electron impact ionization, complexes have been formed in the gas phase between Pb(2+) and a wide range of ligands. The coordinating atoms are oxygen, nitrogen, sulfur, and phosphorus, together with complexes consisting of benzene and argon in association with Pb(2+). Certain ligands are unable to stabilze the metal dication, the most obvious group being water and the lower alcohols, but CS(2) is also unable to form [Pb(CS(2))(N)](2+) complexes. Unlike many other metal dication complexes, those associated with lead appear to exhibit very little chemical reactivity following collisional activation...
January 13, 2005: Journal of Physical Chemistry. A
Yuichi Nakamura, Hisashi Sakamaki, Harumi Mukai, Hiroshi Kojima, Junji Tomiyama, Shin-ichirou Mori, Kiyoshi Hiruma, Norihiko Nakamura, Shigeo Toyota, Hiroyuki Hamaguchi, Kazuo Dans, Kinuko Mitani, Kenji Saito
A feasibility study on high-dose therapy with autologous peripheral blood stem cell transplantation (HDT/PBSCT) was performed in Japanese patients with multiple myeloma (MM). Twenty evaluable patients younger than 65 years old with stage II/III MM were enrolled in this study. Three courses of VAD were used as initial chemotherapy. High-dose etoposide or cyclophosphamide followed by G-CSF was used for PBSCH, and 1.2-89.3 (median 23.4) x 106/kg of CD34+ cells were collected. Single (11 patients) or tandem (9 patients) HDT with melphalan (MEL) 200 mg/m2 or MEL 140 mg/m2 plus TBI 10 Gy were performed...
July 2004: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Mototsugu Muramaki, Isao Hara, Hideaki Miyake, Yuji Yamada, Hiroshi Okada, Sadao Kamidono
The objectives of this study were to evaluate the hematopoietic activity of peripheral blood stem cells (PBSCs) harvested from patients with advanced germ cell tumors after long-term cryopreservation in a mechanical freezer at -80 degrees C. Forty-one patients with advanced tumors were enrolled in this study. Flow cytometric analysis was performed to determine the total cell viability and the viability of CD34 positive cells of PBSCs stored between January 1995 and December 2001. The 41 patients were divided into two groups according to the duration of cryopreservation...
November 2003: Oncology Reports
Shen-Miao Yang, Kai-Yan Liu, Dao-Pei Lu
To achieve efficient peripheral blood stem cell harvest (PBSCH), a simple method to monitor peripheral blood stem/progenitor cells was evaluated. The Sysmex XE-2100 hematology analyzer with an immature information (IMI) channel was used to identify and count the hematopoietic progenitor cell (HPC). Twenty-five donors mobilized with G-CSF in allogeneic and 11 patients in autologous peripheral blood stem cell transplantation (allo-PBSCT and auto-PBSCT) were involved. The HPC, CD34(+) cell and CFU-GM in the peripheral blood and leukapheresis samples were detected during mobilization and harvest...
June 2003: Zhongguo Shi Yan Xue Ye Xue za Zhi
Nobuhiro Kanaji, Ken Ishibashi, Hisamitsu Uno, Norihiko Hino
The chimeric anti-CD20 monoclonal antibody rituximab was recently approved for the treatment of malignant B cell lymphoma. We report the case of a 49 year-old female with advanced mantle cell lymphoma (MCL), who successfully underwent auto-peripheral blood stem cell transplant (auto-PBSCT) in combination with in vivo purging of tumor cells using rituximab. Systemic swelling was detected in her lymph nodes, and she was histologically diagnosed with MCL. From bone marrow involvement and 28% of lymphoma cells in her peripheral blood, she was identified as stage IV of MCL...
December 2002: Gan to Kagaku Ryoho. Cancer & Chemotherapy
K Nakata, H Sakamoto
As a predictor of the timing of peripheral blood stem cell harvest (PBSCH) and the yields of PBSC, the efficacy of measurement of CD34+ cell count in PB has been reported. However this measurement is difficult to carry out on the day of PBSCH in most general hospitals because of not having their own flow cytometry. Therefore in order to evaluate the usefulness of several clinical factors which are easily performed in general hospitals as a guide for PBSCH, we analyzed the correlation between these factors and the harvest efficiency (HE) of PBSCH...
December 1, 2001: Journal of UOEH
M Yamaguchi, M Kimura, Y Watanabe, M Taniguchi, M Masuya, S Kageyama, N Katayama, T Ohno, K Kita, H Shiku
We describe a case of relapsed intravascular lymphomatosis (IVL) successfully treated with autologous PBSCT. A 59-year-old Japanese female patient with IVL who had achieved CR after six courses of biweekly CHOP therapy developed lymphoma. She achieved a second CR after six courses of modified biweekly CHOP therapy, followed by autologous PBSCH and high-dose chemotherapy (CBDCA, VP-16, MCNU, CY) with PBSCT. There has not been any evidence of recurrence 48 months after PBSCT. Our case suggests that PBSC is acceptable as a source for stem cell rescue in IVL...
January 2001: Bone Marrow Transplantation
T Miyazaki, I Matsuda, M Oguri, H Amaya, M Kiyosaki, A Hamada, S Tamaki, E Tashiro, Y Kudo, O Taniguchi, T Nakamura, S Tomoyasu
We analyzed CD34 positive cells in peripheral blood stem cell harvest (PBSCH) using flow cytometry. PBSCH from CD34 positive acute myelogeous leukemia (AML-M2) patient contained 1.87% CD34 positive cells, of which 1.21% was represented by MRD.PBSCH from CD34 positive acute lymphoblast leukemia (ALL) patient contained 3.14% CD34 positive cells, of which 0.11% was accounted for by minimal residual disease (MRD). If PBSCH from CD34 positive acute leukemia patient is analyzed for CD34 monoclonal antibody alone, the presence of CD34 positive MRD may escape attention so that CD34 positive hematopoietic progenitor cells may be overestimated...
January 1, 2001: Journal of Immunological Methods
I D Gowans, M D Hepburn, D M Clark, G Patterson, P S Rawlinson, D T Bowen
Peripheral blood CD34/45+ cell (CD34/45) enumeration is an expensive and labour-intensive investigation but remains the standard assay for optimizing yield and timing of peripheral blood stem cell harvesting (PBSCH). The present study examined the value of the Sysmex SE9000 parameters (WBC, neutrophil count, and immature myeloid index (IMI)) and Sysmex R2000 reticulocyte parameters (absolute, high and medium fluorescence reticulocytes) in predicting the optimum timing of PBSCH in comparison to peripheral blood CD34/45...
October 1999: Clinical and Laboratory Haematology
M E Juweid, R D Blumenthal, W Lew, G Hajjar, A D Rubin, D M Goldenberg
Nine radioimmunotherapy (RAIT)-naive patients with medullary thyroid cancer received high doses of 131I-MN-14 F(ab)2 anti-carcinoembryonic antigen monoclonal antibody (232-486 mCi), five in combination with bone marrow harvest, without prior granulocyte colony stimulating factor (G-CSF) injections (group 1) and the other four using peripheral blood stem cell harvest (PBSCH) preceded by G-CSF administration of 10 microg/kg per day for 5 days for stem cell mobilization, 6-8 days before RAIT (group 2). The amounts of radioactivity (mCi) given in both groups were similar (312 +/- 93 versus 424 +/- 65; P = NS)...
October 1999: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
K Hiruma, S Nakayama, Y Okuyama
Recently, a completely automated procedure, the AutoPBSC program for the COBE Spectra cell separator, has been developed for peripheral blood stem cell harvest (PBSCH). We compared the performance of the AutoPBSC program with the standard mononuclear cell (MNC) program in the same patients and in a donor. Peripheral blood stem cells (PBSC) were collected from 3 patients or a donor alternately using the MNC program and the AutoPBSC program in a course of PBSC mobilization. Equal blood volume was processed from each patient (200 ml/kg) and a donor (150 ml/kg)...
November 1998: Therapeutic Apheresis
J A Snowden, V Nink, M Cooley, J Zaunders, M Keir, L Wright, S T Milliken, P M Brooks, J C Biggs
High-dose chemotherapy with autologous stem cell rescue has been proposed as an intensive therapy for severe rheumatoid arthritis (RA). In view of previous observations of abnormal haemopoiesis in RA patients, the composition and function of peripheral blood stem cell harvests (PBSCH) was investigated. Compared with PBSCH from healthy allogeneic donors mobilized with the same dose of G-CSF (filgrastim; 10 microg/kg/d, n = 14), RA PBSCH (n = 9) contained significantly fewer mononuclear cells (375 v 569 x 10(6)/kg, P = 0...
December 1998: British Journal of Haematology
H Mougi, K Shinmyozu, M Osame
To determine the optimal time of peripheral blood stem cell harvest (PBSCH), we evaluated the usefulness of the detection of immature hematopoietic cells (immature leukocytes and reticulocytes) using newly developed automatic cell analyzers, and compared the results with those of conventional CD34 measurement by flow cytometry (FCM). The presence of immature leukocytes and reticulocytes was calculated as immature leukocyte information (IMI)-positive rate and high fluorescence reticulocyte (HFR)-positive rate by multi-item autoanalyzers...
October 1997: International Journal of Hematology
K Miyamoto, T Otsuka, S Mizuno, K Funatsu, R Inaba
In this study, we investigated CDw90 (Thy-1) expression in CD34+ cells and hematopoietic colony-forming ability in purified CD34+ Thy-1+ and CD34+ Thy-1 fractions in the peripheral blood at the PBSCH. The high proliferative potential colony-forming cells (HPP-CFC), which are considered to be more primitive hematopoietic stem cells, were found more frequently in the purified CD34+ Thy-1+ fraction than in the purified CD34+ Thy-1- fraction. Thus, it is useful to decide the optimal time of PBSCH to measure the CD34+ Thy-1+ cells as well as CD34+ cells in the peripheral blood by flow-cytometry...
January 1997: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
J I Craig, K Langlands, A C Parker, R S Anthony
A total of 96 peripheral blood stem cell harvests (PBSCH) were collected following standard chemotherapy from 27 patients with leukemia, lymphoma, and myeloma. Tumor molecular markers were analyzed in diagnostic samples by Southern blot [immunoglobulin heavy chain (IgHJ), T cell receptor (TcR) delta chain, TcR beta chain] and polymerase chain reaction (PCR) amplification [IgH, TcR delta, t(14;18) translocation] and found in 11 of 22 and 13 of 27 patients, respectively. At a sensitivity of 2 to 5%, Southern blot analysis failed to detect tumor in PBSCH...
August 1994: Experimental Hematology
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