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molecular chaperone and endoplasmic reticulum

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https://www.readbyqxmd.com/read/29155263/identification-and-characterisation-of-the-epididymal-proteins-in-the-lizard-eutropis-carinata-reptilia-squamata-schneider-1801
#1
R Medini, M Bhagya, S Samson
Lizards are seasonal breeders. Cyclic reproductive nature makes lizard as a useful model for the study of the reproductively active protein secretions in the epididymis. During breeding season, the epididymides of the lizard secret proteins that mixes with the spermatozoa and create a favourable environment for sperm maturation. In this spectrum, the aim of this study is to identify and characterize proteins which are present in the lumen of the epididymis of the lizard, E. carinata during the active phase of reproduction...
November 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29145450/enhanced-expression-of-cell-surface-b-cell-receptor-associated-protein-31-contributes-to-poor-survival-of-non-small-cell-lung-carcinoma-cells
#2
Se-Ri Seo, Hyun Min Lee, Hong Seo Choi, Won-Tae Kim, Eun-Wie Cho, Chun Jeih Ryu
B-cell receptor-associated protein 31 (BAP31) is an endoplasmic reticulum (ER) membrane protein which plays a role as a molecular chaperone for the newly synthesized transmembrane proteins. BAP31 is also an important apoptosis regulator for extrinsic apoptosis induction in the ER membrane. Recent studies have shown that BAP31 is also expressed on the surface of embryonic stem cells. However, the function of cell surface BAP31 (csBAP31) still remains unclarified. In an attempt to search for surface markers on tumorspheres, here, we generated monoclonal antibodies (MAbs) against the sphere cells from the non-small cell lung carcinoma cell (NSCLC) line A549...
2017: PloS One
https://www.readbyqxmd.com/read/29129596/amyloid-toxicity-is-enhanced-after-pharmacological-or-genetic-invalidation-of-the-%C3%AF-1-receptor
#3
Tangui Maurice, Manon Strehaiano, Fanny Duhr, Nathalie Chevallier
The sigma-1 receptor (S1R) is a molecular chaperone which activity modulates several intracellular signals including calcium mobilization at mitochondria-associated endoplasmic reticulum membranes. S1R agonists are potent neuroprotectants against neurodegenerative insults and particularly in rodent models of Alzheimer's disease (AD). We here analyzed whether S1R inactivation modifies vulnerability to amyloid toxicity in AD models. Two strategies were used: (1) amyloid β[25-35] (Aβ25-35) peptide (1, 3, 9nmol) was injected intracerebroventricularly in mice treated repeatedly with the S1R antagonist NE-100 or in S1RKO mice, and (2) WT, APPSweInd, S1RKO, and APPSweInd/S1RKO mice were created and female littermates analyzed at 8 months of age...
November 9, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29123254/implications-of-aging-and-the-endoplasmic-reticulum-unfolded-protein-response-on-the-molecular-modality-of-breast-cancer
#4
REVIEW
Rinki Minakshi, Safikur Rahman, Arif Tasleem Jan, Ayyagari Archana, Jihoe Kim
The endoplasmic reticulum (ER) is an important subcellular organelle that is involved in numerous activities required to achieve and maintain functional proteins in addition to its role in the biosynthesis of lipids and as a repository of intracellular Ca(2+). The inability of the ER to cope with protein folding beyond its capacity causes disturbances that evoke ER stress. Cells possess molecular mechanisms aimed at clearing unwanted cargo from the ER lumen as an adaptive response, but failing to do so navigates the system towards cell death...
November 10, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29115545/effects-of-calcium-dependent-molecular-chaperones-and-endoplasmic-reticulum-in-the-amygdala-in-rats-under-single%C3%A2-prolonged-stress
#5
Bing Xiao, Jian-Gang Wang, Fang Han, Yu-Xiu Shi
The purpose of the present study was to investigate the role of endoplasmic reticulum (ER)‑resident molecular chaperone proteins to identify whether these proteins were involved in post‑traumatic stress disorder (PTSD). The present study detected changes of calreticulin (CRT), calnexin (CNX) and ERp57 in the amygdala of rats, which may with aim of providing a novel insight into the modulation effect of amygdala in PTSD. Single‑prolonged stress (SPS) was applied to create the models of PTSD in rats. The expression levels of CRT, CNX and ERp57 were examined using immunohistochemistry or immunofluorescence, western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR)...
November 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29107940/structure-of-the-human-mhc-i-peptide-loading-complex
#6
Andreas Blees, Dovile Januliene, Tommy Hofmann, Nicole Koller, Carla Schmidt, Simon Trowitzsch, Arne Moeller, Robert Tampé
The peptide-loading complex (PLC) is a transient, multisubunit membrane complex in the endoplasmic reticulum that is essential for establishing a hierarchical immune response. The PLC coordinates peptide translocation into the endoplasmic reticulum with loading and editing of major histocompatibility complex class I (MHC-I) molecules. After final proofreading in the PLC, stable peptide-MHC-I complexes are released to the cell surface to evoke a T-cell response against infected or malignant cells. Sampling of different MHC-I allomorphs requires the precise coordination of seven different subunits in a single macromolecular assembly, including the transporter associated with antigen processing (TAP1 and TAP2, jointly referred to as TAP), the oxidoreductase ERp57, the MHC-I heterodimer, and the chaperones tapasin and calreticulin...
November 6, 2017: Nature
https://www.readbyqxmd.com/read/29107114/cancer-chemoprevention-via-activation-of-proteostatic-modules
#7
Aimilia Sklirou, Eleni-Dimitra Papanagnou, Nikolas Fokialakis, Ioannis P Trougakos
Proteins carry out the majority of cellular functions and maintain cellular homeodynamics mostly by participating in multimeric assemblies that operate as protein machines. Proteome quality control is thus critical for cellular functionality, and it is carried out through the curating activity of the proteostasis network (PN). Key components of the PN are the protein synthesis and trafficking modules, the endoplasmic reticulum unfolded protein response, molecular chaperones, and the two main degradation machineries, namely the ubiquitin proteasome and autophagy lysosome pathways...
October 26, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29104090/two-novel-calreticulin-related-molecules-with-microbial-binding-and-phagocytosis-enhancing-capacity-in-the-half-smooth-tongue-sole-cynoglossus-semilaevis
#8
Guanghua Wang, Zengjie Jiang, Shuwen He, Min Zhang
Calreticulin (CRT) is highly conserved chaperone located in the endoplasmic reticulum. It plays important roles in innate immunity. Although various immune-related functions of CRT have been reported in vertebrates and invertebrates, information on the potential functions of teleost CRT is very limited. In the present study, we characterized two calreticulin-related molecules from tongue sole (Cynoglossus semilaevis), calreticulin-like1 and calreticulin-like2 (CsCRTL1 and CsCRTL2). CsCRTL1and CsCRTL2 contain signature CRT motifs that are highly conserved in different species...
November 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29097235/unc93b1-is-essential-for-cytokine-activation-of-five-pamps-in-the-orange-spotted-grouper-epinephelus-coioides
#9
Yaosi Liang, Yu Wang, Liangge He, Jianan He, Wan Peng, Libin Zhou, Yong Zhang, Haifa Zhang, Herong Shi, Haoran Lin, Danqi Lu
Toll-like receptors (TLRs) are important innate immune receptors that recognize multiple pathogen-associated molecular patterns (PAMPs) and activate the immune responses to resist the invasion of pathogens. Many TLRs need assistance from trafficking chaperones to transport to the specific cell compartments and then are processed before they are activated. In this study, we identified an important trafficking chaperone, Unc-93 homolog B1 (unc93b1), from the Epinephelus coioides (orange-spotted grouper). The deduced protein sequence of Eco...
October 31, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29084077/accumulation-of-hla-dr4-in-colonic-epithelial-cells-causes-severe-colitis-in-homozygous-hla-dr4-transgenic-mice
#10
Atsushi Irie, Takahisa Imamura, Yayoi Michibata, Ttatsuko Kubo, Naoki Takeda, Isao Shibuya, Shinji Sogo, Kimi Araki, Yasuharu Nishimura
BACKGROUND: Homozygous HLA-DR4/I-E transgenic mice (tgm) spontaneously developed colitis similar to human ulcerative colitis. We explored whether endoplasmic reticulum stress in colonic epithelial cells due to overexpression of HLA-DR4/I-E was involved in the pathogenesis of colitis. METHODS: Major histocompatibility complex class II transactivator-knockout (CIITAKO) background tgm were established to test the involvement of HLA-DR4/I-E expression in the pathogenesis of colitis...
December 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29071954/-protective-effect-of-acupuncture-serum-derived-from-acute-convulsion-rats-on-cultured-hip-pocampal-neurons-with-seizure-like-discharges-by-regulating-expression-of-endoplasmic-reticulum-stress-inducible-molecular-chaperones
#11
Hui Zhang, Fan Yang, Xu Wu, De-Kai Shen, Hao Chen, Sheng-Bing Wu, Wen-Ping Ang, Xiao-Ge Song, Ming-Yue Xie
OBJECTIVE: To observe the effect of acupuncture serum on the number of apoptosis of the cultured hippocampal neurons with seizure-like discharges and the expression levels of endoplasmic reticulum stress-inducible molecular chaperones glucose-regulated protein 78 (GRP 78), C/EBP homologous protein (CHOP) and cysteine protease protein-12(Caspase-12), so as to reveal its protective mechanism on seizure-induced injury of hippocampal neurons. METHODS: A regular primary culture of neurons derived from the hippocampus of the newly-born SD rats was conducted for 10 days, then these cultured neurons that displayed seizure-like discharges in Mg(2+)-free medium were divided into normal extracellular fluid (medium) group (normal), Mg(2+)-free medium group, acupuncture serum group and non-acupuncture serum group (n=30)...
April 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29069845/the-meta-and-bioinformatics-analysis-of-grp78-expression-in-gastric-cancer
#12
Hua-Chuan Zheng, Bao-Cheng Gong, Shuang Zhao
GRP78 is a molecular chaperone located in endoplasmic reticulum, and induces folding and assembly of newly-synthesized proteins, proteasome degradation of aberrant proteins, and translocation of secretory proteins, autophagy, and epithelial-mesenchymal transition. We performed a systematic meta- and bioinformatics analysis through multiple online databases up to March 14, 2017. It was found that up-regulated GRP78 expression in gastric cancer, compared with normal mucosa at both protein and mRNA levels (p < 0...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29066860/bilberry-extract-administration-prevents-retinal-ganglion-cell-death-in-mice-via-the-regulation-of-chaperone-molecules-under-conditions-of-endoplasmic-reticulum-stress
#13
Orie Nakamura, Satoru Moritoh, Kota Sato, Shigeto Maekawa, Namie Murayama, Noriko Himori, Kazuko Omodaka, Tetsuya Sogon, Toru Nakazawa
PURPOSE: To investigate the effect of bilberry extract anthocyanins on retinal ganglion cell (RGC) survival after optic nerve crush. Additionally, to determine details of the mechanism of the neuroprotective effect of bilberry extract anthocyanins and the involvement of endoplasmic reticulum stress suppression in the mouse retina. MATERIALS AND METHODS: Anthocyanins in bilberry extract (100 mg/kg/day or 500 mg/kg/day) were administrated orally to C57BL/6J mice. The expression levels of various molecular chaperones were assessed with quantitative reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29066620/autoantibodies-against-the-cell-surface-associated-chaperone-grp78-stimulate-tumor-growth-via-tissue-factor
#14
Ali A Al-Hashimi, Paul Lebeau, Fadwa Majeed, Enio Polena, Šárka Lhotak, Celeste A F Collins, Jehonathan H Pinthus, Mario Gonzalez-Gronow, Jen Hoogenes, Salvatore V Pizzo, Mark Crowther, Anil Kapoor, Janusz Rak, Gabriel Gyulay, Sara D'Angelo, Serena Marchio, Renata Pasqualini, Wadih Arap, Bobby Shayegan, Richard C Austin
Tumor cells display on their surface several molecular chaperones that normally reside in the endoplasmic reticulum (ER). Because this display is unique to cancer cells, these chaperones are attractive targets for drug development. Previous epitope-mapping of autoantibodies (AutoAbs) from prostate cancer patients identified glucose-regulated-78 (GRP78) as one such target. Although we previously showed that anti-GRP78 AutoAbs increase tissue factor (TF) procoagulant activity on the surface of tumor cells, the direct effect of TF activation on tumor growth was not examined...
October 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29064368/ampylation-targets-the-rate-limiting-step-of-bip-s-atpase-cycle-for-its-functional-inactivation
#15
Steffen Preissler, Lukas Rohland, Yahui Yan, Ruming Chen, Randy J Read, David Ron
The endoplasmic reticulum (ER)-localized Hsp70 chaperone BiP contributes to protein folding homeostasis by engaging unfolded client proteins in a process that is tightly coupled to ATP binding and hydrolysis. The inverse correlation between BiP AMPylation and the burden of unfolded ER proteins suggests a post-translational mechanism for adjusting BiP's activity to changing levels of ER stress, but the underlying molecular details are unexplored. We present biochemical and crystallographic studies indicating that irrespective of the identity of the bound nucleotide AMPylation biases BiP towards a conformation normally attained by the ATP-bound chaperone...
October 24, 2017: ELife
https://www.readbyqxmd.com/read/29032010/dual-effects-of-insect-nachr-chaperone-ric-3-on-hybrid-receptor-promoting-assembly-on-endoplasmic-reticulum-but-suppressing-transport-to-plasma-membrane-on-xenopus-oocytes
#16
Haibo Bao, Xixia Xu, Wei Liu, Na Yu, Zewen Liu
Resistance to inhibitors of cholinesterase (RIC) -3 promotes the maturation (folding and assembly) of neuronal nicotinic acetylcholine receptors (nAChRs) as a molecular chaperone. The modulation effects of RIC-3 on homomeric α7 nAChRs are always positive, but its effects on heteromeric subtypes are inconsistent among reports. In this study, five RIC-3 isoforms were identified from Locusta migratoria. Four isoforms showed obvious effects on hybrid receptor Locα1/rβ2 expressed in Xenopus oocytes. As a representative, the co-expression of RIC-3v4 exhibited the decreased agonist responses (Imax) on oocytes, lower specific [(3)H]epibatidine binding (Bmax) on plasma membrane protein (PMP), and reduced subunit levels in PMP, which showed that the mature Locα1/rβ2 on the plasma membrane was decreased by the co-expression of RIC-3...
October 11, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/29025875/a-small-molecule-compound-inhibits-a-collagen-specific-molecular-chaperone-and-could-represent-a-potential-remedy-for-fibrosis
#17
Shinya Ito, Koji Ogawa, Koh Takeuchi, Motoki Takagi, Masahito Yoshida, Takatsugu Hirokawa, Shoshiro Hirayama, Kazuo Shin-Ya, Ichio Shimada, Takayuki Doi, Naoki Goshima, Tohru Natsume, Kazuhiro Nagata
Fibrosis can disrupt tissue structure and integrity and impair organ function. Fibrosis is characterized by abnormal collagen accumulation in the extracellular matrix. Pharmacological inhibition of collagen secretion therefore represents a promising strategy for the management of fibrotic disorders, such as liver and lung fibrosis. Hsp47 is an endoplasmic reticulum (ER)-resident collagen-specific molecular chaperone essential for correct folding of procollagen in the ER. Genetic deletion of Hsp47 or inhibition of its interaction with procollagen interferes with procollagen triple helix production, which vastly reduces procollagen secretion from fibroblasts...
October 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28994715/%C3%AE-amyloid-and-the-pathomechanisms-of-alzheimer-s-disease-a-comprehensive-view
#18
REVIEW
Botond Penke, Ferenc Bogár, Lívia Fülöp
Protein dyshomeostasis is the common mechanism of neurodegenerative diseases such as Alzheimer's disease (AD). Aging is the key risk factor, as the capacity of the proteostasis network declines during aging. Different cellular stress conditions result in the up-regulation of the neurotrophic, neuroprotective amyloid precursor protein (APP). Enzymatic processing of APP may result in formation of toxic Aβ aggregates (β-amyloids). Protein folding is the basis of life and death. Intracellular Aβ affects the function of subcellular organelles by disturbing the endoplasmic reticulum-mitochondria cross-talk and causing severe Ca(2+)-dysregulation and lipid dyshomeostasis...
October 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28992123/whole-exome-sequencing-identifies-a-novel-ins-mutation-causative-of-maturity-onset-diabetes-of-the-young-10
#19
Jing Yan, Feng Jiang, Rong Zhang, Tongfu Xu, Zhou Zhou, Wei Ren, Danfeng Peng, Yong Liu, Cheng Hu, Weiping Jia
Monogenic diabetes is often misdiagnosed with type 2 diabetes due to overlapping characteristics. This study aimed to discover novel causative mutations of monogenic diabetes in patients with clinically diagnosed type 2 diabetes and to explore potential molecular mechanisms. Whole-exome sequencing was performed on 31 individuals clinically diagnosed with type 2 diabetes. One novel heterozygous mutation (p.Ala2Thr) in INS was identified. It was further genotyped in an additional case-control population (6523 cases and 4635 controls), and this variant was observed in 0...
August 23, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/28978133/in-depth-phenotyping-of-lymphoblastoid-cells-suggests-selective-cellular-vulnerability-in-marinesco-sj%C3%A3-gren-syndrome
#20
Laxmikanth Kollipara, Stephan Buchkremer, José Andrés González Coraspe, Denisa Hathazi, Jan Senderek, Joachim Weis, René P Zahedi, Andreas Roos
SIL1 is a ubiquitous protein of the Endoplasmic Reticulum (ER) acting as a co-chaperone for the ER-resident chaperone, BiP. Recessive mutations of the corresponding gene lead to vulnerability of skeletal muscle and central nervous system in man (Marinesco-Sjögren syndrome; MSS) and mouse. However, it is still unclear how loss of ubiquitous SIL1 leads to selective vulnerability of the nervous system and skeletal muscle whereas other cells and organs are protected from clinical manifestations. In this study we aimed to disentangle proteins participating in selective vulnerability of SIL1-deficient cells and tissues: morphological examination of MSS patient-derived lymphoblastoid cells revealed altered organelle structures (ER, nucleus and mitochondria) thus showing subclinical vulnerability...
September 15, 2017: Oncotarget
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