keyword
MENU ▼
Read by QxMD icon Read
search

molecular chaperone

keyword
https://www.readbyqxmd.com/read/28431189/to-cure-or-not-to-cure-differential-effects-of-the-chaperone-sorting-factor-cur1-on-yeast-prions-are-mediated-by-the-chaperone-sis1
#1
Yury A Barbitoff, Andrew G Matveenko, Svetlana E Moskalenko, Olga M Zemlyanko, Gary P Newnam, Ayesha Patel, Tatiana A Chernova, Yury O Chernoff, Galina A Zhouravleva
Yeast self-perpetuating protein aggregates (prions) provide a convenient model for studying various components of the cellular protein quality control system. Molecular chaperones and chaperone-sorting factors, such as yeast Cur1 protein, play key role in proteostasis via tight control of partitioning and recycling of misfolded proteins. In this study, we show that, despite the previously described ability of Cur1 to antagonize the yeast prion [URE3], it enhances propagation and phenotypic manifestation of another prion, [PSI(+) ]...
April 21, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28430167/induced-pluripotent-stem-cell-modeling-of-gaucher-s-disease-what-have-we-learned
#2
REVIEW
Dino Matias Santos, Gustavo Tiscornia
Gaucher's disease (GD) is the most frequently inherited lysosomal storage disease, presenting both visceral and neurologic symptoms. Mutations in acid β-glucocerebrosidase disrupt the sphingolipid catabolic pathway promoting glucosylceramide (GlcCer) accumulation in lysosomes. Current treatment options are enzyme replacement therapy (ERT) and substrate reduction therapy (SRT). However, neither of these approaches is effective in treating the neurological aspect of the disease. The use of small pharmacological compounds that act as molecular chaperones is a promising approach that is still experimental...
April 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28428740/protein-quality-control-by-molecular-chaperones-in-neurodegeneration
#3
REVIEW
Aaron Ciechanover, Yong Tae Kwon
Protein homeostasis (proteostasis) requires the timely degradation of misfolded proteins and their aggregates by protein quality control (PQC), of which molecular chaperones are an essential component. Compared with other cell types, PQC in neurons is particularly challenging because they have a unique cellular structure with long extensions. Making it worse, neurons are postmitotic, i.e., cannot dilute toxic substances by division, and, thus, are highly sensitive to misfolded proteins, especially as they age...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28428246/key-features-of-an-hsp70-chaperone-allosteric-landscape-revealed-by-ion-mobility-native-mass-spectrometry-and-double-electron-electron-resonance
#4
Alex L Lai, Eugenia M Clerico, Mandy E Blackburn, Nisha A Patel, Carol V Robinson, Peter P Borbat, Jack H Freed, Lila M Gierasch
Proteins are dynamic entities that populate conformational ensembles, and most functions of proteins depend on their dynamic character. Allostery, in particular, relies on ligand-modulated shifts in these conformational ensembles. Hsp70s are allosteric molecular chaperones with conformational landscapes that involve large rearrangements of their two domains (viz, the nucleotide-binding domain and substrate-binding domain) in response to adenine nucleotides and substrates. However, it remains unclear how the Hsp70 conformational ensemble is populated at each point of the allosteric cycle and how ligands control these populations...
April 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28428045/sco2-deficient-mice-develop-increased-adiposity-and-insulin-resistance
#5
Shauna Hill, Sathyaseelan S Deepa, Kavithalakshmi Sataranatarajan, Pavithra Premkumar, Daniel Pulliam, Yuhong Liu, Vanessa Y Soto, Kathleen E Fischer, Holly Van Remmen
Cytochrome c oxidase (COX) is an essential transmembrane protein complex (Complex IV) in the mitochondrial respiratory electron chain. Mutations in genes responsible for the assembly of COX are associated with Leigh syndrome, cardiomyopathy, spinal muscular atrophy and other fatal metabolic disorders in humans. Previous studies have shown that mice lacking the COX assembly protein Surf1 (Surf1(-/-) mice) paradoxically show a number of beneficial metabolic phenotypes including increased insulin sensitivity, upregulation of mitochondrial biogenesis, induction of stress response pathways and increased lifespan...
April 17, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28427876/polar-n-terminal-residues-conserved-in-type-2-secretion-pseudopilins-determine-subunit-targeting-and-membrane-extraction-steps-during-fibre-assembly
#6
Javier Santos-Moreno, Alexandra East, Ingrid Guilvout, Nathalie Nadeau, Peter J Bond, Guy Tran Van Nhieu, Olivera Francetic
Bacterial type 2 secretion systems (T2SS), type 4 pili (T4P) and archaeal flagella assemble fibres from initially membrane-embedded pseudopilin and pilin subunits. Fibre subunits are made as precursors with positively charged N-terminal anchors, whose cleavage via the prepilin peptidase, essential for pilin membrane extraction and assembly, is followed by N-methylation of the mature (pseudo)pilin N-terminus. The conserved Glu residue at position 5 (E5) of mature (pseudo)pilins is essential for assembly. Unlike T4 pilins, where E5 residue substitutions also abolish N-methylation, the E5A variant of T2SS pseudopilin PulG remains N-methylated, but is affected in interaction with the T2SS component PulM...
April 17, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28427560/stress-adaptive-response-in-ovarian-cancer-drug-resistance-role-of-trap1-in-oxidative-metabolism-driven-inflammation
#7
Maria Rosaria Amoroso, Danilo Swann Matassa, Ilenia Agliarulo, Rosario Avolio, Francesca Maddalena, Valentina Condelli, Matteo Landriscina, Franca Esposito
Metabolic reprogramming is one of the most frequent stress-adaptive response of cancer cells to survive environmental changes and meet increasing nutrient requirements during their growth. These modifications involve cellular bioenergetics and cross talk with surrounding microenvironment, in a dynamic network that connect different molecular processes, such as energy production, inflammatory response, and drug resistance. Even though the Warburg effect has long been considered the main metabolic feature of cancer cells, recent reports identify mitochondrial oxidative metabolism as a driving force for tumor growth in an increasing number of cellular contexts...
2017: Advances in Protein Chemistry and Structural Biology
https://www.readbyqxmd.com/read/28427099/role-of-phosphodiesterases-on-the-function-of-aryl-hydrocarbon-receptor-interacting-protein-aip-in-the-pituitary-gland-and-on-the-evaluation-of-aip-gene-variants
#8
Laura C Hernández-Ramírez, Giampaolo Trivellin, Constantine A Stratakis
Familial isolated pituitary adenoma (FIPA) is caused in about 20% of cases by loss-of-function germline mutations in the AIP gene. Patients harboring AIP mutations usually present with somatotropinomas resulting either in gigantism or young-onset acromegaly. AIP encodes for a co-chaperone protein endowed with tumor suppressor properties in somatotroph cells. Among other mechanisms proposed to explain this function, a regulatory effect over the 3',5'-cyclic adenosine monophosphate (cAMP) signaling pathway seems to play a prominent role...
April 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28425073/molecular-therapy-of-primary-hyperoxaluria
#9
Cristina Martin-Higueras, Armando Torres, Eduardo Salido
During the last few decades, the molecular understanding of the mechanisms involved in primary hyperoxalurias (PHs) has set the stage for novel therapeutic approaches. The availability of PH mouse models has facilitated preclinical studies testing innovative treatments. PHs are autosomal recessive diseases where the enzymatic deficit plays a central pathogenic role. Thus, molecular therapies aimed at restoring such deficit or limiting the consequences of the metabolic derangement could be envisioned, keeping in mind the specific challenges posed by the cell-autonomous nature of the deficiency...
April 19, 2017: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/28424517/molecular-genetics-chaperone-protein-gets-personal
#10
Mark L Siegal
No abstract text is available yet for this article.
April 19, 2017: Nature
https://www.readbyqxmd.com/read/28424476/neurons-export-extracellular-vesicles-enriched-in-cysteine-string-protein-and-misfolded-protein-cargo
#11
Jingti Deng, Carolina Koutras, Julien Donnelier, Mana Alshehri, Maryam Fotouhi, Martine Girard, Steve Casha, Peter S McPherson, Stephen M Robbins, Janice E A Braun
The fidelity of synaptic transmission depends on the integrity of the protein machinery at the synapse. Unfolded synaptic proteins undergo refolding or degradation in order to maintain synaptic proteostasis and preserve synaptic function, and buildup of unfolded/toxic proteins leads to neuronal dysfunction. Many molecular chaperones contribute to proteostasis, but one in particular, cysteine string protein (CSPα), is critical for proteostasis at the synapse. In this study we report that exported vesicles from neurons contain CSPα...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28420739/the-dnak-chaperone-uses-different-mechanisms-to-promote-and-inhibit-replication-of-vibrio-cholerae-chromosome-2
#12
Jyoti K Jha, Mi Li, Rodolfo Ghirlando, Lisa M Miller Jenkins, Alexander Wlodawer, Dhruba Chattoraj
Replication of Vibrio cholerae chromosome 2 (Chr2) depends on molecular chaperone DnaK to facilitate binding of the initiator (RctB) to the replication origin. The binding occurs at two kinds of site, 12-mers and 39-mers, which promote and inhibit replication, respectively. Here we show that DnaK employs different mechanisms to enhance the two kinds of binding. We found that mutations in rctB that reduce DnaK binding also reduce 12-mer binding and initiation. The initiation defect is suppressed by second-site mutations that increase 12-mer binding only marginally...
April 18, 2017: MBio
https://www.readbyqxmd.com/read/28419375/transcriptional-profiling-of-cholinergic-neurons-from-basal-forebrain-identifies-changes-in-expression-of-genes-between-sleep-and-wake-mrna-profiling-in-basal-forebrain-with-sleep-sleep-deprivation
#13
Elena V Nikonova, Jason DA Gilliland, Keith Q Tanis, Alexei A Podtelezhnikov, Alison M Rigby, Raymond J Galante, Eva M Finney, David J Stone, John J Renger, Allan I Pack, Christopher J Winrow
Study objective: To assess differences in gene expression in cholinergic basal forebrain cells between sleeping and sleep deprived mice sacrificed at the same time of day. Methods: Tg(ChAT-eGFP)86Gsat mice expressing eGFP under control of the choline acetyltransferase (Chat) promoter were utilized to guide laser capture of cholinergic cells in basal forebrain. mRNA expression levels in these cells were profiled using microarrays. Gene expression in eGFP(+) neurons was compared (1) to that in eGFP(-) neurons and to adjacent white matter, (2) between 7:00 am (lights on) and 7:00 pm (lights off), (3) between sleep deprived and sleeping animals at 0, 3, 6, 9 hrs from lights on...
April 13, 2017: Sleep
https://www.readbyqxmd.com/read/28418058/crystalline-nanotubular-framework-constructed-by-cucurbit-8-uril-for-selective-co2-adsorption
#14
Pinpin Wang, Yunlong Wu, Yanxia Zhao, Yang Yu, Mingming Zhang, Liping Cao
A crystalline nanotubular framework was constructed by cucurbit[8]uril (CB[8]) molecules in the presence of a molecular chaperone. Compared with amorphous CB[8], the framework exhibits highly selective adsorption for CO2 over N2, CH4, and H2.
April 18, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28414228/molecular-aspects-of-a-robust-assay-for-ferroxidase-function-of-ceruloplasmin
#15
Laura Cortes, Blaine R Roberts, Anthony G Wedd, Zhiguang Xiao
Ceruloplasmin (Cp) is one of the most complex multicopper oxidase enzymes and plays an essential role in the metabolism of iron in mammals. Ferrous ion supplied by the ferroportin exporter is converted by Cp to ferric ion that is accepted by plasma metallo-chaperone transferrin. Study of the enzyme at the atomic and molecular level has been hampered by the lack of a suitable ferrous substrate. We have developed the classic chromophoric complex Fe(II)Hx(Tar)2 (H2Tar, 4-(2-thiazolylazo)resorcinol; x = 0-2; overall charge omitted) as a robust substrate for evaluation of the ferroxidase function of Cp and related enzymes...
April 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28412626/quantification-of-heat-shock-protein-70-and-acetylcholinesterase-over-a-time-course-suggests-environmental-adaptation-in-a-foundational-molluscan-species
#16
Andrew Ravaschiere, Caroline Cutler, Kristi Edleson, Zachery Halem, Henry Magun, Fred Meckler, Rachel Cox
Waterways in urban areas often act as repositories for sewage, industrial waste, and environmental contaminants. In response, inhabitants of these watersheds undergo physiological adaptations specific to their respective environments. Effects of these stressors can be assayed by quantification of various well-documented biomarkers in sentinel species such as the Atlantic Ribbed mussel, Geukensia demissa, a native to the Bronx River Estuary, Bronx, NY, USA. Heat shock protein 70 (Hsp70) is a universally expressed biomarker for an array of environmental stressors including toxins and low dissolved oxygen...
April 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28412368/hsp90-inhibitor-nvp-auy922-induces-cell-apoptosis-by-disruption-of-the-survivin-in-papillary-thyroid-carcinoma-cells
#17
Jinhao Liu, Wei Sun, Wenwu Dong, Zhihong Wang, Yuan Qin, Ting Zhang, Hao Zhang
Heat shock protein 90 (HSP90) is a molecular chaperone required for maintaining the stability and function of signal proteins that plays an important role in promoting the growth and survival of cancer cells. The incidence of papillary thyroid carcinoma (PTC) has been increasing in recent years. The effect of the novel non-geldanamycin HSP90 inhibitor NVP-AUY922 on apoptosis of papillary thyroid carcinoma cells has not been investigated. The influence of AUY922 on the survival of PTC cell lines K1 and IHH4 was evaluated...
April 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28412337/an-overview-on-molecular-chaperones-enhancing-solubility-of-expressed-recombinant-proteins-with-correct-folding
#18
REVIEW
Mina Mamipour, Mohammadreza Yousefi, Mohammad Hasanzadeh
The majority of research topics declared that most of the recombinant proteins have been expressed by Escherichia coli in basic investigations. But the majority of high expressed proteins formed as inactive recombinant proteins that are called inclusion body. To overcome this problem, several methods have been used including suitable promoter, environmental factors, ladder tag to secretion of proteins into the periplasm, gene protein optimization, chemical chaperones and molecular chaperones sets. Co-expression of the interest protein with molecular chaperones is one of the common methods The chaperones are a group of proteins, which are involved in making correct folding of recombinant proteins...
April 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28410515/folate-receptor-targeted-hybrid-lipid-core-nanocapsules-for-sequential-delivery-of-doxorubicin-and-tanespimycin
#19
Biki Gupta, Shiva Pathak, Bijay Kumar Poudel, Shobha Regmi, Hima Bindu Ruttala, Milan Gautam, Jong Seong Lee, Jee-Heon Jeong, Han-Gon Choi, Chul Soon Yong, Jong Oh Kim
When exposed to cancer cells, cytotoxic drugs such as doxorubicin (DOX) can lead to the induction of heat shock protein 90 (Hsp90), a molecular chaperone associated with a number of cancer-related client proteins, and result in cell survival. Co-administration of DOX with tanespimycin (TNP), an Hsp90 inhibitor, can sensitize the cancer cells to the cytotoxic effects of DOX. The effect of such a combination has been found to depend on the schedule of administration. Sequential administration of DOX and TNP has been linked to highly synergistic combination effects...
April 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28408866/post-translational-modifications-and-protein-quality-control-in-motor-neuron-and-polyglutamine-diseases
#20
REVIEW
Fabio Sambataro, Maria Pennuto
Neurodegenerative diseases, including motor neuron and polyglutamine (polyQ) diseases, are a broad class of neurological disorders. These diseases are characterized by neuronal dysfunction and death, and by the accumulation of toxic aggregation-prone proteins in the forms of inclusions and micro-aggregates. Protein quality control is a cellular mechanism to reduce the burden of accumulation of misfolded proteins, a function that results from the coordinated actions of chaperones and degradation systems, such as the ubiquitin-proteasome system (UPS) and autophagy-lysosomal degradation system...
2017: Frontiers in Molecular Neuroscience
keyword
keyword
76712
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"