Elaheh Mirhadi, Anis Askarizadeh, Leila Farhoudi, Mohammad Mashreghi, Saeed Behboodifar, Seyedeh Hoda Alavizadeh, Leila Arabi, Mahmoud Reza Jaafari
In this study, we have developed a redox-sensitive (RS) liposomal doxorubicin formulation by incorporating 10,10'-diselanediylbis decanoic acid (DDA) organoselenium compound as the RS moiety. Hence, several RS liposomal formulations were prepared by using DOPE, HSPC, DDA, mPEG2000 -DSPE, and cholesterol. In situ drug loading using a pH gradient and citrate complex yielded high drug to lipid ratio and encapsulation efficiency (100%) for RS liposomes. Liposomal formulations were characterized in terms of size, surface charge and morphology, drug loading, release properties, cell uptake and cytotoxicity, as well as therapeutic efficacy in BALB/c mice bearing C26 tumor cells...
April 13, 2024: Chemistry and Physics of Lipids