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https://www.readbyqxmd.com/read/28922502/the-three-component-system-esrisr-regulates-a-cell-envelope-stress-response-in-corynebacterium-glutamicum
#1
Britta Kleine, Ava Chattopadhyay, Tino Polen, Daniela Pinto, Thorsten Mascher, Michael Bott, Melanie Brocker, Roland Freudl
When the cell envelope integrity is compromised, bacteria trigger signaling cascades resulting in the production of proteins that counteract these extracytoplasmic stresses. Here, we show that the two-component system EsrSR regulates a cell envelope stress response in the Actinobacterium Corynebacterium glutamicum. The sensor kinase EsrS possesses an amino-terminal phage shock protein C (PspC) domain, a property that sets EsrSR apart from all other two-component systems characterized so far. An integral membrane protein, EsrI, whose gene is divergently transcribed to the esrSR gene locus and which interestingly also possesses a PspC domain, acts as an inhibitor of EsrSR under non-stress conditions...
September 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28922100/frequency-and-antibiotic-resistance-of-bacteria-implicated-in-community-urinary-tract-infections-in-north-aveiro-between-2011-and-2014
#2
Tânia Costa, Inês Linhares, Ricardo Ferreira, Jasmin Neves, Adelaide Almeida
AIMS: The present study aims to evaluate the predominance of uropathogens responsible for urinary tract infection (UTI) and determine their resistance patterns, to assess if the recommended empirical treatment is appropriate for the studied population. Samples were collected in Aveiro (Portugal) from an ambulatory service between June 2011 and June 2014. RESULTS: From the 4,270 positive urine samples for UTI, 3,561 (83%) were from women and only 709 (17%) were from men...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28922029/exploratory-study-into-the-microbiological-quality-of-spinach-and-cabbage-purchased-from-street-vendors-and-retailers-in-johannesburg-south-africa
#3
Erika M du Plessis, Sarasha Govender, Bala Pillay, Lise Korsten
Knowledge of the microbiological quality and prevalence of antibiotic resistance and virulence genes in bacterial isolates from leafy green vegetables supplied by formal suppliers (retailers) and informal suppliers (street vendors) in South Africa is limited. Because leafy vegetables have been implicated in foodborne disease outbreaks worldwide, 180 cabbage and spinach samples were collected from three major retailers and nine street vendors in Johannesburg, South Africa. Escherichia coli and coliforms were enumerated using Petrifilm plates...
September 18, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28920793/multi-drug-resistance-patterns-of-enteric-bacteria-in-two-populations-of-free-ranging-eastern-box-turtles-terrapene-carolina-carolina
#4
Cari Rasmussen, Matthew C Allender, Christopher A Phillips, John Byrd, Terrell Lloyd, Carol Maddox
Gram-negative isolates (n = 84) from 71% of free-ranging Eastern box turtles (Terrapene carolina carolina) in Illinois and Tennessee, United States, demonstrated resistance to at least one antibiotic while 30% of isolates demonstrated resistance to two or more antibiotics. Resistance was observed against cefoxitin, amoxicillin-clavulanic acid, cefazolin, ampicillin, ticarcillin, cefovecin, and ceftiofur. Gram-positive bacteria were isolated from 49 turtles, and all were observed to be resistant to two or more antibiotics...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28919790/exploring-bacterial-outer-membrane-barrier-to-combat-bad-bugs
#5
REVIEW
Ishan Ghai, Shashank Ghai
One of the main fundamental mechanisms of antibiotic resistance in Gram-negative bacteria comprises an effective change in the membrane permeability to antibiotics. The Gram-negative bacterial complex cell envelope comprises an outer membrane that delimits the periplasm from the exterior environment. The outer membrane contains numerous protein channels, termed as porins or nanopores, which are mainly involved in the influx of hydrophilic compounds, including antibiotics. Bacterial adaptation to reduce influx through these outer membrane proteins (Omps) is one of the crucial mechanisms behind antibiotic resistance...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28919615/microbiology-of-chronic-suppurative-otitis-media-in-a-tertiary-care-hospital-in-bangladesh
#6
N Akhtar, P G Datta, M Yasmin, A Naha, A M Jewel, F Hossen, S Ahmed, U K Datta
This cross sectional prospective study was carried out in the Department of Otolaryngology-Head & Neck Surgery, Bangabandhu Sheikh Mujib Medical University (BSMMU) and Department of Microbiology, University of Dhaka from July 2012 to June 2013. The objectives of this study were to identify the common microorganisms involved and the antibiograms of chronic suppurative otitis media (CSOM) patients in this tertiary care hospital in Bangladesh. A total of 117 patients clinically diagnosed of CSOM were enrolled in the study...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28919336/staphylococcus-aureus-dry-surface-biofilms-are-more-resistant-to-heat-treatment-than-traditional-hydrated-biofilms
#7
A Almatroudi, S Tahir, H Hu, D Chowdhury, I B Gosbell, S O Jensen, G S Whiteley, A K Deva, T Glasbey, K Vickery
BACKGROUND: The importance of biofilms to clinical practice is being increasingly realised. Biofilm tolerance to antibiotics is well described but limited work has been conducted on the efficacy of heat disinfection and sterilization against biofilms. AIM: To test the susceptibility of planktonic, hydrated biofilm and dry-surface biofilm forms of Staphylococcus aureus, to dry heat and wet heat treatments. METHODS: S. aureus was grown as both hydrated biofilm and dry-surface biofilm in the CDC biofilm generator...
September 14, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28919044/susceptibility-of-colistin-resistant-pathogens-to-predatory-bacteria
#8
Sonal Dharani, Dong Hyun Kim, Robert M Q Shanks, Yohei Doi, Daniel E Kadouri
The increase in multidrug-resistant Gram-negative bacterial infections has forced the reintroduction of antibiotics such as colistin. However, the spread of plasmid-borne mcr-1 colistin resistance genes have moved us closer to an era of untreatable Gram-negative infections. To evaluate whether predatory bacteria could be used as a potential therapeutic to treat this upcoming threat, the ability of Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus to prey on several clinically relevant mcr-1-positive, colistin-resistant isolates was evaluated...
September 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28918634/an-engineered-probiotic-for-the-inhibition-of-salmonella-via-tetrathionate-induced-production-of-microcin-h47
#9
Jacob D Palmer, Emma Piattelli, Beth McCormick, Mark Silby, Christopher Brigham, Vanni Bucci
Complications arising from antibiotic-resistant bacteria are becoming one of the key issues in modern medicine. Members of drug-resistant Enterobacteriaceae spp. include opportunistic pathogens (e.g. Salmonella spp.) that are among the leading causes of morbidity and mortality worldwide. Overgrowth of these bacteria is considered a hallmark of intestinal dysbiosis. Microcins (small antimicrobial peptides) produced by some gut commensals can potentially cure these conditions by inhibiting these pathogens, and have been proposed as a viable alternative to antibiotic treatment...
September 17, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28918351/susceptibility-profiles-of-bacteria-causing-urinary-tract-infection-in-southern-tunisia
#10
Sonda Guermazi-Toumi, Sirine Boujlel, Mouna Assoudi, Riadh Issaoui, Sonia Tlili, Mohamed Ennaceur Hlaiem
OBJECTIVES: The aim of this study was to determine the epidemiological profile and the antibiotic resistance of bacteria responsible for urinary infections diagnosed in the department of microbiology of the regional hospital Houcine Bouzaiene (Gafsa) in the south west of Tunisia. METHODS: All the cytobacteriological urine samples analyzed from 1 January 2015 to 30 June 2016 were included in the study. The adopted criteria to define urinary infection were: leukocyturia >10(4)/ml and bacteriuria >10(5)CFU/ml...
September 13, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28917968/the-effect-of-fennel-essential-oil-in-combination-with-antibiotics-on-staphylococcus-aureus-strains-isolated-from-carriers
#11
Paweł Kwiatkowski, Magdalena Mnichowska-Polanowska, Agata Pruss, Helena Masiuk, Małgorzata Dzięcioł, Stefania Giedrys-Kalemba, Monika Sienkiewicz
An increase in the number of staphylococcal infections and carriers among medical staff has forced us to seek more and more effective antibacterial agents. Bacteria from the Staphylococcus genus possessing different mechanisms of resistance are the cause of nosocomial infections. The objective of our investigations was susceptibility of S. aureus strains isolated from nasal vestibule of medical students to fennel essential oil. The GC-MS analysis of fennel essential oil revealed eleven constituents among which a majority of trans-anethole (80%) was found...
September 13, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28917952/identification-of-potential-allosteric-communication-pathways-between-functional-sites-of-the-bacterial-ribosome-by-graph-and-elastic-network-models
#12
Pelin Guzel, Ozge Kurkcuoglu
BACKGROUND: Accumulated evidence indicates that bacterial ribosome employs allostery throughout its structure for protein synthesis. The nature of the allosteric communication between remote functional sites remains unclear, but the contact topology and dynamics of residues may play role in transmission of a perturbation to distant sites. METHODS/RESULTS: We employ two computationally efficient approaches - graph and elastic network modeling to gain insights about the allosteric communication in ribosome...
September 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28917929/development-of-carboxymethyl-cellulose-chitosan-hybrid-micro-and-macroparticles-for-encapsulation-of-probiotic-bacteria
#13
P Singh, B Medronho, L Alves, G J da Silva, M G Miguel, B Lindman
Novel carboxymethyl cellulose-chitosan (CMC-Cht) hybrid micro- and macroparticles were successfully prepared in aqueous media either by drop-wise addition or via nozzle-spray methods. The systems were either physically or chemically crosslinked using genipin as the reticulation agent. The macroparticles (ca. 2mm) formed are found to be essentially of the core-shell type, while the microparticles (ca. 5μm) are apparently homogeneous. The crosslinked particles are robust, thermally resistant and less sensitive to pH changes...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917650/dodecyl-sorbitan-ethers-as-antimicrobials-against-gram-positive-bacteria
#14
Dorine Belmessieri, Charlotte Gozlan, Marie-Christine Duclos, Oana Dumitrescu, Gérard Lina, Andreas Redl, Nicolas Duguet, Marc Lemaire
A range of amphiphilic sorbitan ethers has been synthesized in two steps from sorbitan following an acetalization/hydrogenolysis sequence. These sorbitan ethers and the acetal intermediates have been evaluated as antimicrobials against Gram-negative and Gram-positive bacteria. No antimicrobial activity was observed for Gram-negative bacteria. However, the compounds bearing a linear dodecyl chain exhibit antimicrobial activity (MIC as low as 8μg/mL) against Gram-positive bacteria such as Listeria monocytogenes, Enterococcus faecalis and Staphylococcus aureus...
September 7, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28917486/effects-of-the-potential-probiotics-bacillus-aerophilus-kadr3-in-inducing-immunity-and-disease-resistance-in-labeo-rohita
#15
Dharmaraj Ramesh, Sami Souissi, Tharifkhan Shan Ahamed
The aim of the present study was to evaluate the dietary administration of Bacillus aerophilus KADR3 on enhancing the immunity and disease resistance against Aeromonas hydrophila infection in Labeo rohita. B. aerophilus culture supernatant exhibited antagonistic activity against fish pathogenic bacteria in an agar well diffusion assay. Four fish groups were fed with either of following diets containing different concentrations of B. aerophilus KADR3 (cfu g(-1)): 0 (control), 1 × 10(7) (DI), 1 × 10(8) (DII), and 1 × 10(9) (DIII) -cfu g(-1)...
September 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28917263/fourier-transform-infrared-ftir-spectrophotometry-an-ecofriendly-method-for-the-analysis-of-injectable-daptomycin
#16
Eliane Gandolpho Tótoli, Hérida Regina Nunes Salgado
Daptomycin (DPT) is an important antimicrobial agent used in clinical practice because it is very active against several types of medicinally challenging Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci. In addition to concerns about the quality of the analytical methods used in the QC of drugs, there is also concern about the impact of these methods on the environment. The trend toward sustainable consumption is increasingly evident and has forced the pharmaceutical industry to reduce the generation of toxic waste...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28916372/tracking-and-controlling-soft-surface-contamination-in-health-care-settings
#17
Jonathan D Sexton, Amanda M Wilson, Hannah P Sassi, Kelly A Reynolds
BACKGROUND: Study objectives were to track the transfer of microbes on soft surfaces in health care environments and determine the efficiency of an Environmental Protection Agency (EPA)-registered soft surface sanitizer in the health care environment. METHODS: Soft surfaces at 3 health care facilities were sampled for heterotrophic plate count (HPC) bacteria, Staphylococcus spp, Streptococcus pyogenes, and Escherichia coli followed by a tracer study with a virus surrogate seeded onto volunteer hands and commonly touched surfaces...
September 12, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28915922/recent-urbanization-in-china-is-correlated-with-a-westernized-microbiome-encoding-increased-virulence-and-antibiotic-resistance-genes
#18
Kathryn Winglee, Annie Green Howard, Wei Sha, Raad Z Gharaibeh, Jiawu Liu, Donghui Jin, Anthony A Fodor, Penny Gordon-Larsen
BACKGROUND: Urbanization is associated with an increased risk for a number of diseases, including obesity, diabetes, and cancer, which all also show associations with the microbiome. While microbial community composition has been shown to vary across continents and in traditional versus Westernized societies, few studies have examined urban-rural differences in neighboring communities within a single country undergoing rapid urbanization. In this study, we compared the gut microbiome, plasma metabolome, dietary habits, and health biomarkers of rural and urban people from a single Chinese province...
September 15, 2017: Microbiome
https://www.readbyqxmd.com/read/28915875/plectranthus-amboinicus-essential-oil-and-carvacrol-bioactive-against-planktonic-and-biofilm-of-oxacillin-and-vancomycin-resistant-staphylococcus-aureus
#19
Sara Edwirgens Costa Benício Vasconcelos, Hider Machado Melo, Theodora Thays Arruda Cavalcante, Francisco Eduardo Aragão Catunda Júnior, Mário Geraldo de Carvalho, Francisca Gleire Rodrigues Menezes, Oscarina Viana de Sousa, Renata Albuquerque Costa
BACKGROUND: The emergence of multidrug-resistant bacteria is a worldwide concern and in order to find an alternative to this problem, the occurrence of antimicrobial compounds in Plectranthus amboinicus essential oil was investigated. Thus, this study aims to determine susceptibility of Staphylococcus aureus isolated from food to antibiotics, P. amboinicus essential oil (PAEO) and carvacrol. METHODS: Leaves and stem of P. amboinicus were used for extraction of essential oil (PAEO) by hydrodistillation technique and EO chemical analysis was performed by gas chromatography coupled to a mass spectrometer...
September 16, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28915819/bacteriophage-therapy-to-combat-bacterial-infections-in-poultry
#20
REVIEW
Andrzej Wernicki, Anna Nowaczek, Renata Urban-Chmiel
Infections in poultry are an economic and health problem in Europe and worldwide. The most common infections are associated with salmonellosis, colibacillosis, campylobacteriosis, and others. The prevalence of Campylobacter-positive poultry flocks in European countries varies from 18% to 90%. In the United States, the prevalence of infected flocks is nearly 90%. A similar percentage of infection has been noted for salmonellosis (about 75-90%) and E. coli (90-95%). The occurence of Clostridium perfringens is a major problem for the poultry industry, with some estimates suggesting colonization of as many as 95% of chickens, resulting in clinical or subclinical infections...
September 16, 2017: Virology Journal
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