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Biologic Scaffold

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https://www.readbyqxmd.com/read/29785397/whole-genome-sequencing-of-greater-amberjack-seriola-dumerili-for-snp-identification-on-aligned-scaffolds-and-genome-structural-variation-analysis-using-parallel-resequencing
#1
Kazuo Araki, Jun-Ya Aokic, Junya Kawase, Kazuhisa Hamada, Akiyuki Ozaki, Hiroshi Fujimoto, Ikki Yamamoto, Hironori Usuki
Greater amberjack ( Seriola dumerili ) is distributed in tropical and temperate waters worldwide and is an important aquaculture fish. We carried out de novo sequencing of the greater amberjack genome to construct a reference genome sequence to identify single nucleotide polymorphisms (SNPs) for breeding amberjack by marker-assisted or gene-assisted selection as well as to identify functional genes for biological traits. We obtained 200 times coverage and constructed a high-quality genome assembly using next generation sequencing technology...
2018: International Journal of Genomics
https://www.readbyqxmd.com/read/29784964/high-speed-photothermal-off-resonance-atomic-force-microscopy-reveals-assembly-routes-of-centriolar-scaffold-protein-sas-6
#2
Adrian P Nievergelt, Niccolò Banterle, Santiago H Andany, Pierre Gönczy, Georg E Fantner
The self-assembly of protein complexes is at the core of many fundamental biological processes 1 , ranging from the polymerization of cytoskeletal elements, such as microtubules 2 , to viral capsid formation and organelle assembly 3 . To reach a comprehensive understanding of the underlying mechanisms of self-assembly, high spatial and temporal resolutions must be attained. This is complicated by the need to not interfere with the reaction during the measurement. As self-assemblies are often governed by weak interactions, they are especially difficult to monitor with high-speed atomic force microscopy (HS-AFM) due to the non-negligible tip-sample interaction forces involved in current methods...
May 21, 2018: Nature Nanotechnology
https://www.readbyqxmd.com/read/29784586/ultrasound-mediated-gene-delivery-enhances-tendon-allograft-integration-in-mini-pig-ligament-reconstruction
#3
Maxim Bez, Thomas J Kremen, Wafa Tawackoli, Pablo Avalos, Dmitriy Sheyn, Galina Shapiro, Joseph C Giaconi, Shiran Ben David, Jess G Snedeker, Zulma Gazit, Katherine W Ferrara, Dan Gazit, Gadi Pelled
Ligament injuries occur frequently, substantially hindering routine daily activities and sports participation in patients. Surgical reconstruction using autogenous or allogeneic tissues is the gold standard treatment for ligament injuries. Although surgeons routinely perform ligament reconstructions, the integrity of these reconstructions largely depends on adequate biological healing of the interface between the ligament graft and the bone. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would lead to significantly improved ligament graft integration...
April 26, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29782894/screening-of-the-mmv-and-gsk-open-access-chemical-boxes-using-a-viability-assay-developed-against-the-kinetoplastid-crithidia-fasciculata
#4
Wakisa Kipandula, Simon A Young, Stuart A MacNeill, Terry K Smith
Diseases caused by the pathogenic kinetoplastids continue to incapacitate and kill hundreds of thousands of people annually throughout the tropics and sub-tropics. Unfortunately, in the countries where these neglected diseases occur, financial obstacles to drug discovery and technical limitations associated with biochemical studies impede the development of new, safe, easy to administer and effective drugs. Here we report the development and optimisation of a Crithidia fasciculata resazurin viability assay, which is subsequently used for screening and identification of anti-crithidial compounds in the MMV and GSK open access chemical boxes...
May 18, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29782142/projection-based-3d-printing-of-cell-patterning-scaffolds-with-multi-scale-channels
#5
Dai Xue, Yancheng Wang, Jiaxin Zhang, Deqing Mei, Yue Wang, Shaochen Chen
To fully actualize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organs-on-a-chip systems, cells need to be patterned such that they can precisely mimic natural microenvironments in vitro. Despite increasing interest in this area, patterning cells at multiscales (~10 μm - 10 mm) remains a significant challenge in bioengineering. Here we report a projection-based 3D printing system that achieves rapid and high-resolution fabrication of hydrogel scaffolds featuring intricate channels for multi-scale cell patterning...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29781355/development-and-biological-validation-of-a-cyclic-stretch-culture-system-for-the-ex-vivo-engineering-of-tendons
#6
Manuela Teresa Raimondi, Matteo Laganà, Claudio Conci, Michele Crestani, Alessia Di Giancamillo, Francesca Gervaso, Daniela Deponti, Federica Boschetti, Michele M Nava, Chiara Scandone, Cinzia Domeneghini, Alessandro Sannino, Giuseppe M Peretti
INTRODUCTION: An innovative approach to the treatment of tendon injury or degeneration is given by engineered grafts, made available through the development of bioreactors that generate tendon tissue in vitro, by replicating in vivo conditions. This work aims at the design of a bioreactor capable of applying a stimulation of cyclic strain on cell constructs to promote the production of bioartificial tissue with mechanical and biochemical properties resembling those of the native tissue...
May 1, 2018: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/29781274/the-synthesis-of-branched-trehalose-glycolipids-and-their-mincle-agonist-activity
#7
Jessie H Bird, Ashna A Khan, Naoya Nishimura, Sho Yamasaki, Mattie S M Timmer, Bridget L Stocker
The macrophage inducible C-type lectin (Mincle) is a pattern recognition receptor that recognises trehalose dimycolate (TDM), and trehalose dibehenate (TDB) and related trehalose diesters, and thus represents a promising target for the development of vaccine adjuvants based on the trehalose glycolipid scaffold. To this end, we report on the synthesis of a series of long-chain α-branched, β-modified trehalose monoesters and diesters to explore how glycolipid structure affects signalling through Mincle. Key steps in our synthetic strategy include a Fráter-Seebach α-alkylation to install the C20 aliphatic lipid on a malic acid derivative, and the formation of a β, γ-epoxide as an intermediate from which modifications to the β-position of the lipid can be made...
May 21, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29780512/iriomoteolides-novel-chemical-tools-to-study-actin-dynamics
#8
A Unzue, R Cribiú, M M Hoffman, T Knehans, K Lafleur, A Caflisch, C Nevado
Despite its promising biological profile, the cellular targets of iriomoteolide-3a, a novel 15-membered macrolide isolated from Amphidinium sp., have remained unknown. A small library of non-natural iriomoteolide-3a analogues is presented here as a result of a novel, highly convergent, catalysis-based scaffold-diversification campaign, which revealed the suitable sites for chemical editing in the original core. We provide compelling experimental evidence for actin as one of iriomoteolides' primary cellular targets, establishing the ability of these secondary metabolites to inhibit cell migration, induce severe morphological changes in cells and cause a reversible cytoplasmic retraction and reduction of F-actin fibers in a time and dose dependent manner...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29777811/electrospun-chitosan-polycaprolactone-hyaluronic-acid-bilayered-scaffold-for-potential-wound-healing-applications
#9
Amit Chanda, Jaideep Adhikari, Aritri Ghosh, Sougata Roy Chowdhury, Sabu Thomas, Pallab Datta, Prosenjit Saha
Fabrication of mechanically stable, biocompatible bilayered polymeric scaffold consisting of chitosan(CS)/polycaprolactone(PCL) and hyaluronic acid(HA) using less toxic solvent system is presented in this study. Electrospinning technique to make the scaffold was used followed by morphological, physiochemical and mechanical characterizations. Average fiber diameter of CS/PCL-HA bilayered scaffold was found 362.2 ± 236 nm which is in the range of collagen fiber found in the extracellular matrices. Enhanced swelling, degradation, hydrophilicity and water vapour transmission rate were found for the bilayered scaffold compared to that of the PCL and CS-PCL scaffolds...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777616/regenerative-endodontics-a-review
#10
REVIEW
S G Kim, M Malek, A Sigurdsson, L M Lin, B Kahler
The European Society of Endodontology and the American Association for Endodontists have released position statements and clinical considerations for regenerative endodontics. There is increasing literature on this field since the initial reports of Iwaya et al. (2001) and Banchs & Trope (2004). Endogenous stem cells from an induced periapical bleeding and scaffolds using blood clot, platelet rich plasma or platelet rich fibrin have been utilized in regenerative endodontics. This approach has been described as a 'paradigm shift" and considered the first treatment option for immature teeth with pulp necrosis...
May 19, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29775937/design-synthesis-and-biological-evaluation-of-a-series-of-novel-2-benzamide-4-6-oxy-n-methyl-1-naphthamide-pyridine-derivatives-as-potent-fibroblast-growth-factor-receptor-fgfr-inhibitors
#11
Manman Wei, Xia Peng, Li Xing, Yang Dai, Ruimin Huang, Meiyu Geng, Ao Zhang, Jing Ai, Zilan Song
Starting from the phase II clinical FGFR inhibitor lucitanib (2), we conducted a medicinal chemistry approach by opening the central quinoline skeleton coupled with a scaffold hopping process thus leading to a series of novel 2-benzamide-4-(6-oxy-N-methyl-1-naphthamide)-pyridine derivatives. Compound 25a was identified to show selective and equally high potency against FGFR1/2 and VEGFR2 with IC50 values less than 5.0 nM. Significant antiproliferative effects on both FGFR1/2 and VEGFR2 aberrant cancer cells were observed...
May 15, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29775305/one-pot-synthesis-of-four-chlorin-derivatives-by-a-divergent-ylide
#12
Junior Gonzales, Naga Venkata Satya Dinesh Kumar Bhupathiraju, Daniel Hart, Man Yuen, Maria Pia Sifuentes, Bleron Samarxhiu, Mark Maranan, Naxhije Berisha, James Batteas, Charles Michael Drain
Chlorins have unique photophysical properties that are exploited in diverse biological and materials applications. De novo chlorin synthesis with specific exocyclic motifs can be challenging and many are not stable to photobleaching and/or oxidation. A facile approach to a stable synthetic chlorin with a fused N-methyl pyrrolidine uses cyclo addition of a sarcosine-based azomethine ylide on 5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (TPPF20) is reported, but this approach has limitations. We report the synthesis of stable chlorin scaffolds starting with TPPF20 using a new glycine-based N-(hydroxymethyl)-N-methelenemethanideaminium ylide...
May 18, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29772461/quickening-translational-design-of-resorbable-synthetic-vascular-grafts
#13
REVIEW
Chelsea E T Stowell, Yadong Wang
Traditional tissue-engineered vascular grafts have yet to gain wide clinical use. The difficulty of scaling production of these cell- or biologic-based products has hindered commercialization. In situ tissue engineering bypasses such logistical challenges by using acellular resorbable scaffolds. Upon implant, the scaffolds become remodeled by host cells. This review describes the scientific and translational advantages of acellular, synthetic vascular grafts. It surveys in vivo results obtained with acellular synthetics over their fifty years of technological development...
May 5, 2018: Biomaterials
https://www.readbyqxmd.com/read/29772346/tackling-bioactive-glass-excessive-in-vitro-bioreactivity-preconditioning-approaches-for-cell-culture-tests
#14
REVIEW
Francesca E Ciraldo, Elena Boccardi, Virginia Melli, Fabian Westhauser, Aldo R Boccaccini
Bioactive glasses (BGs) are being increasingly considered for biomedical applications in bone and soft tissue replacement approaches thanks to their ability to form strong bonding with tissues. However, due to their high reactivity once in contact with water-based solutions BGs rapidly exchange ions with the surrounding environment leading in most cases to an undesired increase of the pH under static in vitro conditions (due to alkaline ion "burst release"), making difficult or even impossible to perform cell culture studies...
May 14, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29768120/three-dimensionally-printed-pearl-powder-poly-caprolactone-composite-scaffolds-for-bone-regeneration
#15
Xu Zhang, Xiaoyu Du, Dejian Li, Rongguang Ao, Bin Yu, Baoqing Yu
Pearl has great potential as a natural biomaterial for bone tissue engineering, but it suffers from low porosity, difficulty in molding, and poor anti-buckling property. In this study, we used the 3-D printing technique to fabricate original pearl powder and PCL composite scaffolds with different concentrations of pearl powder. The four groups of scaffolds were termed PCL, 30% Pearl/PCL, 50% Pearl/PCL and 80% Pearl/PCL scaffolds according to the proportion of pearl powder. The samples were systematically investigated by scanning electron microscopy (SEM), wide-angle XRD, liquid substitution, Zwick static materials testing, and energy dispersive X-ray analysis...
May 16, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29767463/mechanical-and-biological-properties-of-a-biodegradable-mg-zn-ca-porous-alloy
#16
Yong-Qiang Zhang, Yang Li, Huan Liu, Jing Bai, Ni-Rong Bao, Yue Zhang, Peng He, Jian-Ning Zhao, Li Tao, Feng Xue, Guang-Xin Zhou, Gen-Tao Fan
OBJECTIVES: As promising alternative to current metallic biomaterials, the porous Mg scaffold with a 3-D open-pore framework has drawn much attention in recent years due to its suitable biodegradation, biocompatibility, and mechanical properties for human bones. This experiment's aim is to study the mechanical properties, biosafety, and osteogenesis of porous Mg-Zn alloy. METHODS: A porous Mg-2Zn-0.3Ca (wt%) alloy was successfully prepared by infiltration casting, and the size of NaCl particles was detected by a laser particle size analyzer...
May 16, 2018: Orthopaedic Surgery
https://www.readbyqxmd.com/read/29767291/bioengineered-scaffolds-for-stem-cell-applications-in-tissue-engineering-and-regenerative-medicine
#17
Maryam Rahmati, Cristian Pablo Pennisi, Ali Mobasheri, Masoud Mozafari
Stem cell-based therapies, harnessing the ability of stem cells to regenerate damaged or diseased tissues, are under wide-ranging consideration for regenerative medicine applications. However, limitations concerning poor cell persistence and engraftment upon cell transplantation still remain. During the recent years, several types of biomaterials have been investigated to control the fate of the transplanted stem cells, aiming to increase their therapeutic efficiency. In the present chapter we focus on the general properties of some of these biomaterials, which include polymers, ceramics, and nano-biomaterials...
May 17, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29765407/effects-of-transplanted-heparin-poloxamer-hydrogel-combining-dental-pulp-stem-cells-and-bfgf-on-spinal-cord-injury-repair
#18
Lihua Luo, Abdullkhaleg Ali Albashari, Xiaoyan Wang, Ling Jin, Yanni Zhang, Lina Zheng, Jianjian Xia, Helin Xu, Yingzheng Zhao, Jian Xiao, Yan He, Qingsong Ye
Spinal cord injury (SCI) is one of serious traumatic diseases of the central nervous system and has no effective treatment because of its complicated pathophysiology. Tissue engineering strategy which contains scaffolds, cells, and growth factors can provide a promising treatment for SCI. Hydrogel that has 3D network structure and biomimetic microenvironment can support cellular growth and embed biological macromolecules for sustaining release. Dental pulp stem cells (DPSCs), derived from cranial neural crest, possess mesenchymal stem cell (MSC) characteristics and have an ability to provide neuroprotective and neurotrophic properties for SCI treatment...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29763808/design-synthesis-and-evaluation-of-novel-multi-target-directed-ligands-for-treatment-of-alzheimer-s-disease-based-on-coumarin-and-lipoic-acid-scaffolds
#19
Leili Jalili-Baleh, Hamid Forootanfar, Tuba Tüylü Küçükkılınç, Hamid Nadri, Zahra Abdolahi, Alieh Ameri, Mandana Jafari, Beyza Ayazgok, Maryam Baeeri, Mahban Rahimifard, Syed Nasir Abbas Bukhari, Mohammad Abdollahi, Mohammad Reza Ganjali, Saeed Emami, Mehdi Khoobi, Alireza Foroumadi
A novel series of coumarin-lipoic acid conjugates were synthesized via cycloaddition click reaction to find out new multi-target-directed ligands (MTDLs) for treatment of Alzheimer's disease (AD). All of synthesized compounds were screened for neuroprotective and anti-cholinesterase activities. Based on primary screening, two compounds (5 and 11) were subjected to further biological evaluations. In particular, compound 11 which was the most potent AChE inhibitor showed good inhibitory effect on Aβ-aggregation and intracellular ROS (reactive oxygen species) formation, as well as the ability of selective bio-metal chelation and neuroprotection against H2 O2 - and Aβ1-42 -induced cytotoxicity...
May 2, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29763517/novel-engineered-tendon-fibrocartilage-bone-composite-with-cyclic-tension-for-rotator-cuff-repair
#20
Qian Liu, Taku Hatta, Jun Qi, Haoyu Liu, Andrew R Thoreson, Peter C Amadio, Steven L Moran, Scott P Steinmann, Anne Gingery, Chunfeng Zhao
Surgical repair of rotator cuff tears presents a significant clinical challenge with high failure rates and inferior functional outcomes. Graft augmentation improves repair outcomes, however currently available grafting materials have limitations. While cell-seeded decellularized tendon slices may facilitate cell infiltration, promote tendon incorporation and preserve original mechanical strength, the unique fibrocartilage zone is yet to be successfully reestablished. In this study, we investigated the biological and mechanical properties of an engineered tendon-fibrocartilage-bone composite (TFBC) with cyclic tension (3% strain, 0...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
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