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https://www.readbyqxmd.com/read/29782554/hijacking-of-multiple-phospholipid-biosynthetic-pathways-and-induction-of-membrane-biogenesis-by-a-picornaviral-3cd-protein
#1
Sravani Banerjee, David Aponte-Diaz, Calvin Yeager, Suresh D Sharma, Gang Ning, Hyung S Oh, Qingxia Han, Masato Umeda, Yuji Hara, Robert Y L Wang, Craig E Cameron
RNA viruses induce specialized membranous structures for use in genome replication. These structures are often referred to as replication organelles (ROs). ROs exhibit distinct lipid composition relative to other cellular membranes. In many picornaviruses, phosphatidylinositol-4-phosphate (PI4P) is a marker of the RO. Studies to date indicate that the viral 3A protein hijacks a PI4 kinase to induce PI4P by a mechanism unrelated to the cellular pathway, which requires Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, GBF1, and ADP ribosylation factor 1, Arf1...
May 21, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29781995/an-ecdysone-receptor-based-singular-gene-switch-for-deliberate-expression-of-transgene-with-robustness-reversibility-and-negligible-leakiness
#2
Seoghyun Lee, Minho Won, Ran Hee Hwang, Gang Min Hur, Hyunju Ro
Precise control of transgene expression is desirable in biological and clinical studies. However, because the binary feature of currently employed gene switches requires the transfer of two therapeutic expression units concurrently into a single cell, the practical application of the system for gene therapy is limited. To simplify the transgene expression system, we generated a gene switch designated as pEUI(+) encompassing a complete set of transgene expression modules in a single vector. Comprising of the GAL4 DNA-binding domain and modified EcR (GvEcR), a minimal VP16 activation domain fused with a GAL4 DNA-binding domain, as well as a modified Drosophila ecdysone receptor (EcR), the newly developed singular gene switch is highly responsive to the administration of a chemical inducer in a time- and dosage-dependent manner...
May 7, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29780485/unexpected-solvation-stabilisation-of-ions-in-a-protic-ionic-liquid-insights-disclosed-by-a-bond-energetic-study
#3
Zhen Wang, Feixiang Gao, Pengju Ji, Jin-Pei Cheng
Equilibrium acidities (p K a s) of 42 organic acids were precisely determined in protic ionic liquid (PIL) [DBUH][OTf]. Surprisingly, the often seen homoassociation complication during the p K a measurement of O-H acids in DMSO was not detected in [DBUH][OTf], implying that the incipient oxanion should be better solvation-stabilized by the PIL, although its "apparent" dielectric constant is much lower than that of the conventional molecular solvent DMSO. Evidence showing that the solute ions in the PIL are also free from other specific ion associations like ion-pairing is further demonstrated by the identical p K a s of protic amine salts bearing largely different counter-anions...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780479/pentacyclic-adenine-a-versatile-and-exceptionally-bright-fluorescent-dna-base-analogue
#4
Mattias Bood, Anders F Füchtbauer, Moa S Wranne, Jong Jin Ro, Sangamesh Sarangamath, Afaf H El-Sagheer, Déborah L M Rupert, Rachel S Fisher, Steven W Magennis, Anita C Jones, Fredrik Höök, Tom Brown, Byeang Hyean Kim, Anders Dahlén, L Marcus Wilhelmsson, Morten Grøtli
Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qAnitro , pA allows accurate distance and orientation determination via Förster resonance energy transfer (FRET) measurements...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29779436/early-lupus-project-one-year-follow-up-of-an-italian-cohort-of-patients-with-systemic-lupus-erythematosus-of-recent-onset
#5
G D Sebastiani, I Prevete, A Iuliano, M Piga, F Iannone, L Coladonato, M Govoni, A Bortoluzzi, M Mosca, C Tani, A Doria, L Iaccarino, A Tincani, M Fredi, F Conti, F R Spinelli, M Galeazzi, F Bellisai, A Zanetti, G Carrara, C A Scirè, A Mathieu
Objective To describe the clinical and serological features of a prospectively followed cohort of early diagnosed systemic lupus erythematosus (SLE) patients during a one-year follow-up period. Methods SLE patients with disease duration less than 12 months were consecutively enrolled in a multicentre, prospective study. At study entry and then every 6 months, a large panel of data was recorded. Results Of 260 patients enrolled, 185 had at least 12 months of follow-up; of these, 84.3% were female, 92.4% were Caucasians...
January 1, 2018: Lupus
https://www.readbyqxmd.com/read/29778657/efficacy-of-antifungal-impregnated-cement-spacer-against-chronic-fungal-periprosthetic-joint-infections-after-total-knee-arthroplasty
#6
Jong-Keun Kim, Do-Yoon Lee, Dong-Wan Kang, Du-Hyun Ro, Myung Chul Lee, Hyuk-Soo Han
BACKGROUND: Although two-stage exchange arthroplasty is considered a treatment of choice for chronic features of fungal PJI (periprosthetic joint infection), there is no consensus for local use of antifungal agent. The purpose of this study was to evaluate the efficacy of antifungal-impregnated cement spacer (AICS). METHODS: Nine patients who were diagnosed and treated for chronic fungal PJI after TKA in a single center from January 2001 to December 2016 were enrolled...
May 16, 2018: Knee
https://www.readbyqxmd.com/read/29777135/n-methyl-d-aspartate-receptor-subtype-2b-antagonist-ro-25-6981-attenuates-neuropathic-pain-by-inhibiting-postsynaptic-density-95-expression
#7
Ling-Er Huang, Shao-Hui Guo, Lalita Thitiseranee, Yan Yang, Yan-Feng Zhou, Yong-Xing Yao
Postsynaptic density-95 (PSD-95) is a synaptic scaffolding protein that plays a crucial role in the development of neuropathic pain. However, the underlying mechanism remains unclear. To address the role of PSD-95 in N-methyl-D-aspartate receptor subtype 2B (NR2B) -mediated chronic pain, we investigated the relationship between PSD-95 activation and NR2B function in the spinal cord, by using a rat model of sciatic nerve chronic constriction injury (CCI). We demonstrate that the expression levels of total PSD-95 and cAMP response element binding protein (CREB), as well as phosphorylated NR2B, PSD-95, and CREB, in the spinal dorsal horn, and the interaction of NR2B with PSD-95 were increased in the CCI animals...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29774167/complete-hypokalemic-quadriparesis-as-a-first-presentation-of-sj%C3%A3-gren-syndrome
#8
Jason An, Branko Braam
Rationale: We hope to increase awareness that hypokalemic paralysis may be the first presentation of Sjögren syndrome, for which potassium-sparing diuretics can be an effective adjunct to potassium replenishment. Presenting concerns: A 73-year-old female presented to a peripheral hospital with quadriparesis and a critically low serum potassium of 1.6 mmol/L with U waves on the electrocardiogram (ECG). The initial arterial blood gas showed a pH of 7.19, bicarbonate of 13 mEq/L, and a CO2 of 35 mm Hg...
2018: Canadian Journal of Kidney Health and Disease
https://www.readbyqxmd.com/read/29765886/reference-value-for-infrapatellar-branch-of-saphenous-nerve-conduction-study-cadaveric-and-clinical-study
#9
Keon-Tae Kim, Yong-Ki Kim, Jung Ro Yoon, Yundam Ko, Myung Eun Chung
Objective: To determine the optimal stimulation and recording site for infrapatellar branch of saphenous nerve (IPBSN) conduction studies by a cadaveric study, and to confirm that obtained location is practically applicable to healthy adults. Methods: Twelve lower limbs from six cadavers were studied. We defined the optimal stimulation site as the point IPBSN exits the sartorius muscle and the distance or ratio were measured on the X- and Y-axis based on the line connecting the medial and lateral poles of the patella...
April 2018: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/29765879/quantitative-measurement-of-muscle-atrophy-and-fat-infiltration-of-the-supraspinatus-muscle-using-ultrasonography-after-arthroscopic-rotator-cuff-repair
#10
Yong Ki Kim, Eun Seok Choi, Keon Tae Kim, Jung Ro Yoon, Sang Han Chae
Objective: To investigate the utility of ultrasonography to objectively examine morphological changes (i.e., muscle atrophy and fatty infiltration) of the supraspinatus muscle. Methods: Thirty-four patients were prospectively enrolled in this study. The degrees of muscle atrophy and fat infiltration were measured using ultrasonography 3-4 months after arthroscopic supraspinatus tendon repair. Shoulder function (i.e., shoulder active range of motion, visual analogue scale, and constant score) was examined...
April 2018: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/29764151/a-full-dimensional-potential-energy-surface-and-quantum-dynamics-of-inelastic-collision-process-for-h-2-hf
#11
Dongzheng Yang, Jing Huang, Junxiang Zuo, Xixi Hu, Daiqian Xie
A full-dimensional ab initio potential energy surface for the H2 -HF van der Waals complex was constructed by employing the coupled-cluster singles and doubles with noniterative inclusion of connected triples with augmented correlation-consistent polarised valence quadruple-zeta basis set plus bond functions. Using the improved coupled-states approximation including the nearest neighbor Coriolis couplings, we calculated the state-to-state scattering dynamics for pure rotational and ro-vibrational energy transfer processes...
May 14, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29761590/the-interaction-of-anti-diabetic-%C3%AE-glucosidase-inhibitors-and-gut-bacteria-%C3%AE-glucosidase
#12
Kemin Tan, Christine Tesar, Rosemarie Wilton, Robert P Jedrzejczak, Andrzej Joachimiak
Carbohydrate hydrolyzing α-glucosidases are commonly found in microorganisms present in the human intestine microbiome. We have previously reported crystal structures of an α-glucosidase from the human gut bacterium Blaubia (Ruminococcus) obeum (Ro-αG1) and its substrate preference/specificity switch. This novel member of the GH31 family is a structural homolog of human intestinal maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) with a highly conserved active site that is predicted to be common in Ro-αG1 homologs among other species that colonize the human gut...
May 15, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29757698/prediction-of-non-linear-pharmacokinetics-in-humans-of-an-antibody-drug-conjugate-adc-when-evaluation-of-higher-doses-in-animals-is-limited-by-tolerability-case-study-with-an-anti-cd33-adc
#13
Isabel Figueroa, Doug Leipold, Steve Leong, Bing Zheng, Montserrat Carrasco-Triguero, Aimee Fourie-O'Donohue, Katherine R Kozak, Keyang Xu, Melissa Schutten, Hong Wang, Andrew G Polson, Amrita V Kamath
For antibody-drug conjugates (ADCs) that carry a cytotoxic drug, doses that can be administered in preclinical studies are typically limited by tolerability, leading to a narrow dose range that can be tested. For molecules with non-linear pharmacokinetics (PK), this limited dose range may be insufficient to fully characterize the PK of the ADC and limits translation to humans. Mathematical PK models are frequently used for molecule selection during preclinical drug development and for translational predictions to guide clinical study design...
May 14, 2018: MAbs
https://www.readbyqxmd.com/read/29755149/influence-of-integrated-membrane-treatment-on-the-phytotoxicity-of-wastewater-from-the-coke-industry
#14
Marzena Smol, Dariusz Włóka, Maria Włodarczyk-Makuła
In this paper, coke wastewater that had passed through biological and integrated membrane processes (filtration on sand bed-reverse osmosis) was chosen to assess the phytotoxicity of selected industrial wastewater with regard to the test plant- Vicia faba . An innovative research technique in vitro test was conducted in a large scale phytothrone chamber on two matrices: cotton and Murashige and Skoog Basal Medium (MSBM). The toxicity of wastewater was evaluated for samples: (1) treated in the treatment plant by biological processes, (2) filtrated through a sand bed and filtrated (3) reverse osmosis (RO) membrane...
2018: Water, Air, and Soil Pollution
https://www.readbyqxmd.com/read/29753504/extracorporeal-life-support-for-adults-with-refractory-septic-shock
#15
Sun Kyun Ro, Wan Kee Kim, Ju Yong Lim, Jae Suk Yoo, Sang-Bum Hong, Joon Bum Kim
OBJECTIVE: Although the use of extracorporeal membrane oxygenation (ECMO) in shock patients is increasing worldwide, studies concerning this treatment for adult septic shock are limited. This study aimed to analyze the outcome of venoarterial ECMO in adult patients with septic shock refractory to conventional treatment. METHODS: A total of 71 consecutive patients who presented with septic shock and underwent venoarterial ECMO were reviewed. Clinical parameters were compared between survivors and nonsurvivors...
April 7, 2018: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29752942/structure-based-protein-engineering-of-bacterial-%C3%AE-xylosidase-to-increase-the-production-yield-of-xylobiose-from-xylose
#16
Seokho Hong, Myungok Kyung, Inseong Jo, Yong-Ro Kim, Nam-Chul Ha
Xylobiose consists of two molecules of xylose and has been highly recognized as a food supplement because it possesses high prebiotic functions. β-xylosidase exhibits enzymatic activity to hydrolyze xylobiose, and the enzyme can also catalyze the reverse reaction in the presence of high concentrations of xylose. Previously, β-xylosidase from Bacillus pumilus IPO (BpXynB), belonging to GH family 43, was employed to produce xylobiose from xylose. To improve the enzymatic efficiency, this study determined the high-resolution structure of BpXynB in a complex with xylobiose and engineered BpXynB based on the structures...
May 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29752644/a-new-candidate-laser-dye-based-1-4-bis-%C3%AE-2-naphthothiazolyle-vinyl-benzene-spectroscopic-behavior-laser-parameters-and-excitation-energy-transfer
#17
Abeer N Al-Romaizan, Mahmoud A Hussein
A new candidate laser dye based 1,4-bis[β-(2-naphthothisolyl) vinyl] benzene (BNTVB) were prepared, and characterized in various organic solvents. The center polarity is less sensitive than electronic absorption. A red shift was noticed in the fluorescence spectra (ca. 40 nm) with increment in the solvent's polarity, this means that BNTVB's polarity appreciates upon excitation. The dipole moment of ground state (μg ) and the excited singlet state dipole moment (μe ) are determined from Kawski - Chamma and Bakshiev-Viallet equations using the disparity of Stokes shift with solvent polarity function of ε (dielectric constant) and n (refractive index) of the solvent...
May 12, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29752278/identification-and-validation-of-radiographic-enhancement-for-reliable-differentiation-of-cd117-benign-renal-oncocytoma-and-chromophobe-renal-cell-carcinoma
#18
Jay Amin, Bo Xu, Shervin Badkhshan, Terrance T Creighton, Daniel Abbotoy, Christine Murekeyisoni, Kristopher Attwood, Thomas Schwaab, Craig Hendler, Michael Petroziello, Charles L Roche, Eric C Kauffman
PURPOSE: The diagnostic differential for CD117/KIT(+) oncocytic renal tumor biopsies is limited to benign renal oncocytoma (RO) vs. chromophobe renal cell carcinoma (ChRCC), however further differentiation is often challenging and requires surgical resection.  We investigated clinical variables that might improve preoperative differentiation of CD117(+) RO vs. ChRCC to avoid the need for benign tumor resection. EXPERIMENTAL DESIGN: 124 nephrectomy patients from a single institute with 133 RO or ChRCC tumors were studied...
May 11, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29749963/formation-of-spherical-shaped-gan-and-inn-quantum-dots-on-curved-sin-si-surface
#19
Ilgyu Choi, Hyunjoong Lee, Cheul-Ro Lee, Kwang-Un Jeong, Jin Soo Kim
This paper reports the formation of GaN and InN quantum dots (QDs) with symmetric spherical shapes, grown on SiN/Si(111). Spherical QDs are grown by modulating initial growth behavior via gallium and indium droplets functioning as nucleation sites for QDs. Field-emission scanning electron microscope (FE-SEM) images show that GaN and InN QDs are formed on curved SiN/Si(111) instead of on a flat surface similar to balls on a latex mattress. This is considerably different from the structural properties of In(Ga)As QDs grown on GaAs or InP...
May 11, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29748100/impact-of-magnetic-resonance-imaging-on-gross-tumor-volume-delineation-in-non-spine-bony-metastasis-treated-with-stereotactic-body-radiation-therapy
#20
Srinivas Raman, Lee Chin, Darby Erler, Eshetu G Atenafu, Patrick Cheung, William Chu, Hans Chung, Andrew Loblaw, Ian Poon, Joel Rubenstein, Hany Soliman, Arjun Sahgal, Chia-Lin Tseng
PURPOSE: This study investigates the inter-observer variability of contouring non-spine bone metastases using the planning CT alone vs. the addition of MRI T1 and T2 imaging sequences. METHODS AND MATERIALS: 10 cases of non-spine bone metastases treated with SBRT at our institution were selected. The gross tumor volume (GTV) for each case was delineated by six SBRT radiation oncologists (RO) and one diagnostic radiologist (DR) on the treatment planning CT. After a minimum of three months, each case was re-contoured on the CT fused with a MRI T1 sequence followed by a MRI T2 sequence...
March 17, 2018: International Journal of Radiation Oncology, Biology, Physics
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