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https://www.readbyqxmd.com/read/28938786/prediction-of-egg-freshness-during-storage-using-electronic-nose
#1
Samuel M Yimenu, J Y Kim, B S Kim
The aim of the present study was to investigate the potential of a fast gas chromatography (GC) e-nose for freshness discrimination and for prediction of storage time as well as sensory and internal quality changes during storage of hen eggs. All samples were obtained from the same egg production farm and stored at 20 °C for 20 d. Egg sampling was conducted every 0, 3, 6, 9, 12, 16, and 20 d. During each sampling time, 4 egg cartons (each containing 10 eggs) were randomly selected: one carton for Haugh units, one carton for sensory evaluation and 2 cartons for the e-nose experiment...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938751/new-permeable-cryoprotectant-free-vitrification-method-for-native-human-sperm
#2
J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#3
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938668/systematic-review-and-meta-analysis-cholecystectomy-and-the-risk-of-cholangiocarcinoma
#4
Jianping Xiong, Yaqin Wang, Hanchun Huang, Jin Bian, Anqiang Wang, Junyu Long, Ying Zheng, Xinting Sang, Yiyao Xu, Xin Lu, Haitao Zhao
Studies have reported that cholecystectomy may increase the risk of cholangiocarcinoma. However, this association is controversial. Thus, we conducted a systematic review and meta-analysis to explore the relationship between cholecystectomy and the risk of cholangiocarcinoma. Relevant studies were identified by searching PubMed, EMBASE, ISI Web of Science published before February 2017. We used the random effects model proposed by DerSimonian and Laird to quantify the relationship between cholecystectomy and risk of cholangiocarcinoma...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938651/positive-transcription-elongation-factor-b-p-tefb-is-a-therapeutic-target-in-human-multiple-myeloma
#5
Yu Zhang, Liang Zhou, Yun Leng, Yun Dai, Robert Z Orlowski, Steven Grant
The role of the positive RNA Pol II regulator, P-TEFb (positive transcription elongation factor b), in maintenance of the anti-apoptotic protein Mcl-1 and bortezomib (btz) resistance was investigated in human multiple myeloma (MM) cells. Mcl-1 was up-regulated in all MM lines tested, including bortezomib-resistant lines, human MM xenograft mouse models, and primary CD138(+) MM cells. Mcl-1 over-expression significantly reduced bortezomib lethality, indicating a functional role for Mcl-1 in bortezomib resistance...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938643/serum-peptidome-profiling-for-the-diagnosis-of-colorectal-cancer-discovery-and-validation-in-two-independent-cohorts
#6
Hao Wang, Chenghua Luo, Shengtao Zhu, Honghong Fang, Qing Gao, Siqi Ge, Haixia Qu, Qingwei Ma, Hongwei Ren, Youxin Wang, Wei Wang
Colorectal cancer (CRC) is one of the most common malignant neoplasms worldwide. Except for the existing fecal occult blood test, colonoscopy and sigmoidoscopy, no widely accepted in vitro diagnostic methods have been available. To identify potential peptide biomarkers for CRC, serum samples from a discovery cohort (100 CRC patients and 100 healthy controls) and an independent validation cohort (91 CRC patients and 91 healthy controls) were collected. Peptides were fractionated by weak cation exchange magnetic beads (MB-WCX) and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938490/expression-of-contactin-4-is-associated-with-malignant-behavior-in-pheochromocytomas-and-paragangliomas
#7
Lucie Evenepoel, Francien H van Nederveen, Lindsey Oudijk, Thomas G Papathomas, David F Restuccia, Eric J T Belt, Wouter W de Herder, Richard A Feelders, Gaston J H Franssen, Marc Hamoir, Dominique Maiter, Aurel Perren, Henri J L M Timmers, Susanne van Eeden, Laurent Vroonen, Selda Aydin, Mercedes Robledo, Miikka Vikkula, Ronald R de Krijger, Winand N M Dinjens, Alexandre Persu, Esther Korpershoek
Context: Pheochromocytomas and paragangliomas (PPGL) are rare neuroendocrine, usually benign tumors. Currently, the only reliable criterion of malignancy is the presence of metastases. Objective: The aim was to identify genes associated with malignancy in PPGL. Design: Transcriptomic profiling was performed on 40 benign and 11 malignant PPGL. Genes showing a significantly different expression between benign and malignant PPGL with a ratio ≥ 4 were confirmed, and subsequently tested in an independent series by qRT-PCR...
August 17, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938284/longitudinal-validation-of-the-promis-physical-function-item-bank-in-upper-extremity-trauma
#8
Aaron J Kaat, Nan E Rothrock, Mark S Vrahas, Robert V OʼToole, Sarah K Buono, Timothy Zerhusen, Richard C Gershon
OBJECTIVES: To evaluate the reliability, validity, and responsiveness of the Patient-Reported Outcomes Measurement Information System (PROMIS) Upper Extremity Computer Adaptive Test (UE-CAT) and the 8-item Physical Function short form (PF-SF8a) for monitoring outcomes after musculoskeletal injuries in upper extremity trauma patients. DESIGN: Prospective cohort study. SETTING: Two Level-I trauma centers. PATIENTS: Eligible consecutive patients were approached and 424 consented at time 1 (median 9...
October 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28938266/identification-of-an-optimal-cut-off-for-drain-fluid-amylase-on-postoperative-day-1-for-predicting-clinically-relevant-fistula-after-distal-pancreatectomy-a-multi-institutional-analysis-and-external-validation
#9
Laura Maggino, Giuseppe Malleo, Claudio Bassi, Valentina Allegrini, Joal D Beane, Ross M Beckman, Bofeng Chen, Euan J Dickson, Jeffrey A Drebin, Brett L Ecker, Douglas L Fraker, Michael G House, Nigel B Jamieson, Ammar A Javed, Stacy J Kowalsky, Major K Lee, Matthew T McMillan, Robert E Roses, Roberto Salvia, Vicente Valero, Lavanniya K P Velu, Christopher L Wolfgang, Amer H Zureikat, Charles M Vollmer
OBJECTIVE: The aim of this study was to investigate the relationship between drain fluid amylase value on the first postoperative day (DFA1) and clinically relevant fistula (CR-POPF) after distal pancreatectomy (DP), and to identify the cut-off of DFA1 that optimizes CR-POPF prediction. BACKGROUND: DFA1 is a well-recognized predictor of CR-POPF after pancreatoduodenectomy, but its role in DP is largely unexplored. METHODS: DFA1 levels were correlated with CR-POPF in 2 independent multi-institutional sets of DP patients: developmental (n = 338; years 2012 to 2017) and validation cohort (n = 166; years 2006 to 2016)...
September 21, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28938184/preliminary-evidence-that-reactivity-to-uncertain-threat-is-an-endophenotype-for-alcohol-use-disorder
#10
Stephanie M Gorka, Stewart A Shankman
BACKGROUND: Having an exaggerated reactivity to threats that are uncertain (U-threat) may facilitate the initiation and maintenance of excessive alcohol use in some individuals. This abnormality may not just be a concomitant for alcohol use disorder (AUD), but also an endophenotype for AUD. METHOD: The aim of the current study was therefore to provide a preliminary test of whether U-threat is an endophenotype for AUD using several of the endophenotype criteria outlined by Gottesman and Gould (2003)...
September 14, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28938167/a-method-for-measuring-manual-position-control
#11
Cory Adam Potts, Alexander A Brown, Stanislaw Solnik, David A Rosenbaum
There is no generally accepted method for measuring manual position control. We developed a method for doing so. We asked university students to hold a handle that had one rotational degree of freedom. The angular position of the handle depended on the degree of pronation-supination of the forearm. The subjects' task was to hold the handle as steadily as possible to keep a needle positioned in a pie-shaped target zone on a computer screen. If the needle remained in the zone for 0.5s, the gain of the feedback loop increased; otherwise the gain decreased or remained at the starting value of 1...
September 19, 2017: Acta Psychologica
https://www.readbyqxmd.com/read/28938147/development-of-an-experimental-model-of-burst-fracture-with-damage-characterization-of-the-vertebral-bodies-under-dynamic-conditions
#12
A Germaneau, T Vendeuvre, M Saget, P Doumalin, J C Dupré, F Brémand, F Hesser, C Brèque, P Maxy, M Roulaud, O Monlezun, P Rigoard
BACKGROUND: Burst fractures represent a significant proportion of fractures of the thoracolumbar junction. The recent advent of minimally invasive techniques has revolutionized the surgical treatment of this type of fracture. However mechanical behaviour and primary stability offered by these solutions have to be proved from experimental validation tests on cadaveric specimens. Therefore, the aim of this study was to develop an original and reproducible model of burst fracture under dynamic impact...
September 13, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28938049/detection-of-mutations-in-gyrb-using-denaturing-high-performance-liquid-chromatography-dhplc-among-salmonella-enterica-serovar-typhi-and-paratyphi-a
#13
Ruchi Gupta, Rajni Gaind, Laishram Chandreshwor Singh, Bianca Paglietti, Monorama Deb, Salvatore Rubino, John Wain, Seemi Farhat Basir
Background: Fluoroquinolone resistance is mediated by mutations in the quinolone-resistance determining region (QRDR) of the topoisomerase genes. Denaturing high performance liquid chromatography (DHPLC) was evaluated for detection of clinically important mutations in gyrB among Salmonella. Methods: Salmonella Typhi and S. Paratyphi A characterised for mutation in QRDR of gyrA, parC and parE were studied for mutation in gyrB by DHPLC and validated by sequencing...
December 1, 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28938028/association-between-implant-abutment-microgap-and-implant-circularity-to-bacterial-leakage-an-in-vitro-study-using-tapered-connection-implants
#14
Eduardo Cláudio Lopes de Chaves E Mello Dias, Marcelo Sperandio, Marcelo Henrique Napimoga
PURPOSE: The aim of this study was to evaluate the microgap between the abutment and implant as well as the circularity of implant platforms and associating conformational errors with bacterial microleakage in tapered connection implant systems. MATERIALS AND METHODS: Four brands of implants with a tapered abutment connection were tested. Bacterial leakage was assessed using 0.3 μL of Escherichia coli suspension inoculated into the abutment screw chamber of the implants, which were then torqued and incubated at 37°C for 14 days...
September 22, 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28938018/genome-wide-identification-of-bacterial-plant-colonization-genes
#15
Benjamin J Cole, Meghan E Feltcher, Robert J Waters, Kelly M Wetmore, Tatiana S Mucyn, Elizabeth M Ryan, Gaoyan Wang, Sabah Ul-Hasan, Meredith McDonald, Yasuo Yoshikuni, Rex R Malmstrom, Adam M Deutschbauer, Jeffery L Dangl, Axel Visel
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system...
September 22, 2017: PLoS Biology
https://www.readbyqxmd.com/read/28937943/multiomics-analysis-of-tumor-microenvironment-reveals-gata2-and-mirna-124-3p-as-potential-novel-biomarkers-in-ovarian-cancer
#16
Esra Gov, Medi Kori, Kazim Yalcin Arga
Ovarian cancer is a common and, yet, one of the most deadly human cancers due to its insidious onset and the current lack of robust early diagnostic tests. Tumors are complex tissues comprised of not only malignant cells but also genetically stable stromal cells. Understanding the molecular mechanisms behind epithelial-stromal crosstalk in ovarian cancer is a great challenge in particular. In the present study, we performed comparative analyses of transcriptome data from laser microdissected epithelial, stromal, and ovarian tumor tissues, and identified common and tissue-specific reporter biomolecules-genes, receptors, membrane proteins, transcription factors (TFs), microRNAs (miRNAs), and metabolites-by integration of transcriptome data with genome-scale biomolecular networks...
September 22, 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28937870/student-led-screening-of-school-children-for-refractive-error-correction
#17
Dipesh Bhattarai, Subodh Gnyawali, Arpan Silwal, Sudan Puri, Anita Shrestha, Man Bahadur Kunwar, Madan Prasad Upadhyay
PURPOSE: Detection and correction of uncorrected refractive errors among 12 million children is an overwhelming task for the 500 currently available eye-care professionals in Nepal. A mid-term review of VISION 2020 goals in Nepal has recommended utilizing alternative human resources that could screen children for visual impairment. This study was conducted to assess the validity of vision screening of school children by trained high school students when compared to optometrist testing as the gold standard...
September 22, 2017: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/28937783/validation-of-the-missouri-osteochondral-allograft-preservation-system-for-the-maintenance-of-osteochondral-allograft-quality-during-prolonged-storage
#18
Aaron M Stoker, James P Stannard, Keiichi Kuroki, Chantelle C Bozynski, Ferris M Pfeiffer, James L Cook
BACKGROUND: Fresh osteochondral allografts (OCAs) are limited in availability. The Missouri Osteochondral Allograft Preservation System (MOPS) has been reported to effectively preserve OCAs twice as long as current tissue bank protocols in preclinical studies. HYPOTHESIS: The viable chondrocyte density (VCD) in OCAs preserved for up to 70 days using the MOPS will not be significantly different from day 0, and the VCD in MOPS-preserved OCAs will be significantly higher than for standard tissue bank preservation...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28937647/identification-of-dna-binding-proteins-using-mixed-feature-representation-methods
#19
Kaiyang Qu, Ke Han, Song Wu, Guohua Wang, Leyi Wei
DNA-binding proteins play vital roles in cellular processes, such as DNA packaging, replication, transcription, regulation, and other DNA-associated activities. The current main prediction method is based on machine learning, and its accuracy mainly depends on the features extraction method. Therefore, using an efficient feature representation method is important to enhance the classification accuracy. However, existing feature representation methods cannot efficiently distinguish DNA-binding proteins from non-DNA-binding proteins...
September 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28937557/clinical-validation-of-a-test-for-the-diagnosis-of-vaginitis
#20
Gilbert Donders, Pedro Vieira Baptista
No abstract text is available yet for this article.
October 2017: Obstetrics and Gynecology
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