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https://www.readbyqxmd.com/read/29165614/long-term-health-in-recipients-of-transplanted-in-vitro-propagated-spermatogonial-stem-cells
#1
Callista L Mulder, Lisa A E Catsburg, Yi Zheng, Cindy M de Winter-Korver, Saskia K M van Daalen, Madelon van Wely, Steven Pals, Sjoerd Repping, Ans M M van Pelt
STUDY QUESTION: Is testicular transplantation of in vitro propagated spermatogonial stem cells associated with increased cancer incidence and decreased survival rates in recipient mice? SUMMARY ANSWER: Cancer incidence was not increased and long-term survival rate was not altered after transplantation of in vitro propagated murine spermatogonial stem cells (SSCs) in busulfan-treated recipients as compared to non-transplanted busulfan-treated controls. WHAT IS KNOWN ALREADY: Spermatogonial stem cell autotransplantation (SSCT) is a promising experimental reproductive technique currently under development to restore fertility in male childhood cancer survivors...
November 18, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29163522/the-vagus-nerve-in-the-neuro-immune-axis-implications-in-the-pathology-of-the-gastrointestinal-tract
#2
REVIEW
Bruno Bonaz, Valérie Sinniger, Sonia Pellissier
The vagus nerve (VN) is the longest nerve of the organism and a major component of the parasympathetic nervous system which constitutes the autonomic nervous system (ANS), with the sympathetic nervous system. There is classically an equilibrium between the sympathetic and parasympathetic nervous systems which is responsible for the maintenance of homeostasis. An imbalance of the ANS is observed in various pathologic conditions. The VN, a mixed nerve with 4/5 afferent and 1/5 efferent fibers, is a key component of the neuro-immune and brain-gut axes through a bidirectional communication between the brain and the gastrointestinal (GI) tract...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29162385/new-method-of-detecting-hydrophobic-interaction-between-c-terminal-binding-domain-and-biomacromolecules
#3
JiaFeng Huang, RiBang Wu, CuiLing Wu, Dan Liu, Jiang Zhang, BinQiang Liao, Ming Lei, Xiao Xiao, ChangBei Ma, HaiLun He
The C-terminal domains of proteases play crucial roles in hydrolysis, substrate adsorption and targeted binding. Identifying and characterizing interactions between C-terminal domains and biomacromolecules can help to examine the diversity as well as the substrate-binding ability of C-terminal domains and to explore novel functions. The bacterial pre-peptidase C-terminal (PPC) domain is a typical C-terminal domain normally found at the C-terminus of bacterial secreted proteases. In this work, we successfully demonstrated that 8-anilinonaphthalene-1-sulfonic acid (ANS) could be used to rapidly determine the interactions between this C-terminal domain and biomacromolecules...
November 18, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29161454/religious-and-spiritual-practices-among-home-less-urban-american-indians-and-alaska-natives-with-severe-alcohol-problems
#4
Dennis C Wendt, Susan E Collins, Lonnie A Nelson, Kelly Serafini, Seema L Clifasefi, Dennis M Donovan
Engagement in religious and spiritual practices may be protective for homeless individuals with alcohol-related problems. However, little is known in this regard for urban-dwelling American Indians and Alaska Natives (AI/ANs) who have disproportionately high rates of homelessness and co-occurring alcohol use problems. Using secondary data from a nonrandomized controlled study testing a Housing First intervention, AI/AN participants (n = 52) and non-AI/AN participants (n = 82) were compared on demographic variables, alcohol use problems, religious affiliations, and religious/spiritual practices (importance, frequency, and type)...
2017: American Indian and Alaska Native Mental Health Research: the Journal of the National Center
https://www.readbyqxmd.com/read/29157670/presence-of-comorbidities-and-prognosis-of-clinical-symptoms-in-knee-and-or-hip-osteoarthritis-a-systematic-review-and-meta-analysis
#5
REVIEW
Patrick Calders, Ans Van Ginckel
OBJECTIVE: (i) To determine the association between the presence of comorbidities and severity of pain and physical dysfunction in people with knee and/or hip osteoarthritis; (ii) to explore associations between specific comorbidities (cardiac disease and/or hypertension, diabetes, depression, and back pain) and symptom severity. METHODS: Studies were identified through systematic searches in four electronic databases and grey literature, and, subsequently, methodologically appraised...
October 31, 2017: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/29154021/diagnosis-of-fanconi-anaemia-by-ionising-radiation-or-mitomycin-c-induced-micronuclei
#6
Flavia Zita Francies, Rosalind Wainwright, Janet Poole, Kim De Leeneer, Ilse Coene, Greet Wieme, Hélène A Poirel, Bénédicte Brichard, Stephanie Vermeulen, Anne Vral, Jacobus Slabbert, Kathleen Claes, Ans Baeyens
Fanconi Anaemia (FA) is an autosomal recessive disorder characterised by defects in DNA repair, associated with chromosomal instability and cellular hypersensitivity to DNA cross-linking agents such as mitomycin C (MMC). The FA repair pathway involves complex DNA repair mechanisms crucial for genomic stability. Deficiencies in DNA repair genes give rise to chromosomal radiosensitivity. FA patients have shown increased clinical radiosensitivity by exhibiting adverse normal tissue side-effects. The study aimed to investigate chromosomal radiosensitivity of homozygous and heterozygous carriers of FA mutations using three micronucleus (MN) assays...
November 8, 2017: DNA Repair
https://www.readbyqxmd.com/read/29153107/interictal-cardiac-autonomic-nervous-system-disturbances-in-dogs-with-idiopathic-epilepsy
#7
M Musteata, D Mocanu, G D Stanciu, M Armasu, G Solcan
Autonomic nervous system (ANS) activity in the interictal period (InIp) in dogs with presumed idiopathic epilepsy (pIE) was assessed using heart rate variability (HRV) analysis. The HRVs obtained from 28 pIE dogs with interictal epileptic discharges (InIEd; 11 with treatment and 17 without treatment) detected on electroencephalography (EEG) were compared with those obtained from 13 healthy dogs. On electrocardiographic (ECG) study, the P wave dispersion (PWD; P<0.001), P max (P=0.004) and corrected QT interval (QTc; P=0...
October 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29149840/geometric-morphometrics-reveals-shifts-in-flower-shape-symmetry-and-size-following-gene-knockdown-of-cycloidea-and-anthocyanidin-synthase
#8
Brent A Berger, Vincent A Ricigliano, Yoland Savriama, Aedric Lim, Veronica Thompson, Dianella G Howarth
BACKGROUND: While floral symmetry has traditionally been assessed qualitatively, recent advances in geometric morphometrics have opened up new avenues to specifically quantify flower shape and size using robust multivariate statistical methods. In this study, we examine, for the first time, the ability of geometric morphometrics to detect morphological differences in floral dorsoventral asymmetry following virus-induced gene silencing (VIGS). Using Fedia graciliflora Fisch. & Meyer (Valerianaceae) as a model, corolla shape of untreated flowers was compared using canonical variate analysis to knockdown phenotypes of CYCLOIDEA2A (FgCYC2A), ANTHOCYANIDIN SYNTHASE (FgANS), and empty vector controls...
November 17, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29146222/nursing-staff-distress-associated-with-neuropsychiatric-symptoms-in-young-onset-dementia-and-late-onset-dementia
#9
Jeannette C L van Duinen-van den IJssel, Ans J M J Mulders, Martin Smalbrugge, Sandra A Zwijsen, Britt Appelhof, Syste U Zuidema, Marjolein E de Vugt, Frans R J Verhey, Christian Bakker, Raymond T C M Koopmans
OBJECTIVE: The aims of this study were (1) to investigate the relationship between different neuropsychiatric symptoms (NPS) and the level of distress experienced by nurses caring for residents with young-onset dementia (YOD) and (2) to compare these findings with those for nurses caring for residents with late-onset dementia (LOD). DESIGN/SETTING: This is a retrospective study conducted in Dutch long-term care facilities. Data were used from the Behavior and Evolution of Young-ONset Dementia studies (BEYOND) Parts I and II and the WAAL Behavior in Dementia-II (Waalbed-II) study...
November 14, 2017: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/29143574/investigating-the-preventive-effects-of-baicalin-and-gallocatechin-aginst-glyoxal-induced-cystatin-aggregation
#10
Aamir Sohail, Waseem Feeroze Bhat, Mohammad Furkan, Aaliya Shah, Bilqees Bano
Several mammalian proteins form pathological deposits under nonphysiological conditions that are associated with many degenerative diseases. Protein aggregation is associated with aging, as well as a variety of diseases, including cystic fibrosis, amyotrophic lateral sclerosis (ALS), and hypertrophic cardiomyopathy. There is a lack of any potential anti-amyloidogenic agents and therapeutics till date. Polyphenols have been accredited with myriad biological effects. An analysis of the effects of natural agents like baicalin (BC) and gallocatechin (GC) on aggregation process can open new avenues for the treatment of protein misfolding diseases...
November 16, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29143123/recovery-of-the-cardiac-autonomic-nervous-and-vascular-system-after-maximal-cardiopulmonary-exercise-testing-in-recreational-athletes
#11
Heidi Weberruss, Johannes Maucher, Renate Oberhoffer, Jan Müller
OBJECTIVE: The body's adaptation to physical exercise is modulated by sympathetic and parasympathetic (vagal) branches of the autonomic nervous system (ANS). Heart rate variability (HRV), the beat-to-beat variation of the heart, is a proxy measure for ANS activity, whereas blood pressure (BP) is an indicator for cardiovascular function. Impaired vagal activity and lower BP is already described after exercise. However, inconsistent results exist about how long vagal recovery takes and how long post-exercise hypotension persists...
November 15, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29138038/low-dose-betamethasone-acetate-for-fetal-lung-maturation-in-preterm-sheep
#12
Augusto F Schmidt, Matthew W Kemp, Judith Rittenschober-Böhm, Paranthaman S Kannan, Haruo Usuda, Masatoshi Saito, Lucy Furfaro, Shimpei Watanabe, Sarah Stock, Boris W Kramer, John P Newnham, Suhas G Kallapur, Alan H Jobe
BACKGROUND: Antenatal steroids (ANS) are standard of care for women at risk of preterm delivery; however ANS dosing and formulation have not been adequately evaluated. The standard clinical 2-dose treatment with betamethasone-acetate+betamethasone-phosphate (Beta-P+Beta-Ac) is more effective than 2 doses of Beta-P for inducing lung maturation in preterm fetal sheep. We hypothesized that the slowly-released Beta-Ac component induces similar lung maturation to Beta-P+Beta-Ac with decreased dose and fetal exposure...
November 11, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29133821/incidence-of-asymptomatic-neurosyphilis-in-serofast-chinese-syphilis-patients
#13
Sheng Nan Cai, Jing Long, Chen Chen, Gang Wan, Wen Hui Lun
More new diagnosed syphilis cases were reported in china, the incidence and relevant factors of asymptomatic neurosyphilis (ANS) in serofast syphilis patients were unclear. Clinical and laboratory data of 402 Human Immunodeficiency Virus (HIV) negative, serofast syphilis patients, who underwent lumbar puncture at the Peking University Ditan Teaching Hospital between September 2008 and August 2016, were collected. Incidence of ANS was verified and the relevant factors were further analyzed. According to the ANS criteria, 139 (34...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29130651/further-delineation-of-the-gdf6-related-multiple-synostoses-syndrome
#14
Paulien A Terhal, Nienke E Verbeek, Nine Knoers, Rutger J A J Nievelstein, Ans van den Ouweland, Ralph J Sakkers, Lucienne Speleman, Gijs van Haaften
A mutation in GDF6 was recently found to underlie a multiple synostoses syndrome. In this report, we describe the second family with GDF6-related multiple synostoses syndrome (SYNS4), caused by a novel c.1287C>A/p.Ser429Arg mutation in GDF6. In addition to synostoses of carpal and/or tarsal bones, at least 6 of 10 affected patients in this family have been diagnosed with mild to moderate hearing loss. In four of them otosclerosis was said to be present, one patient had hearing loss due to severe stapes fixation at the age of 6 years, providing evidence that hearing loss in the GDF6-related multiple synostoses syndrome can be present in childhood...
November 12, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/29128322/role-of-1-2-dioleoyl-3-trimethylammonium-propane-dotap-in-%C3%AE-lactoglobulin-aggregation
#15
Baoliang Ma, Xiaofei Wang, Yujie Liu, Zhihong Li, Yujie Liu, Fan Zhang
Protein aggregation is a prevalent phenomenon. It is important to study protein aggregation under different solution conditions. In this study, using 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectra, we investigated the critical micelle concentration (CMC) of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). We also studied the effects of DOTAP on amyloid aggregation of β-lactoglobulin using intrinsic fluorescence spectra, circular dichroism spectra, thioflavin T fluorescence, a Congo red binding assay and transmission electron microscopy...
November 8, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/29122618/effects-of-tai-chi-exercise-on-cancer-related-fatigue-in-patients-with-nasopharyngeal-carcinoma-undergoing-chemoradiotherapy-a-randomized-controlled-trial
#16
Wei Zhou, Yong-Hui Wan, Qian Chen, Yan-Ru Qiu, Xiao-Min Luo
CONTEXT: Tai Chi exercise has been shown to improve cancer-related fatigue (CRF) and autonomic nervous system (ANS) balance in some cancer patients or survivors, however, such effects are yet to be verified in nasopharyngeal carcinoma (NPC) patients undergoing chemoradiotherapy. OBJECTIVES: To explore the effects of Tai Chi exercise on CRF in NPC patients undergoing chemoradiotherapy and then to evaluate ANS information indicated by heart rate variability (HRV) parameters and their association with CRF...
November 6, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/29117951/long-term-benefit-of-enzyme-replacement-therapy-in-pompe-disease-a-5-year-prospective-study
#17
Esther Kuperus, Michelle E Kruijshaar, Stephan C A Wens, Juna M de Vries, Marein M Favejee, Jan C van der Meijden, Dimitris Rizopoulos, Esther Brusse, Pieter A van Doorn, Ans T van der Ploeg, Nadine A M E van der Beek
OBJECTIVE: To determine the effect of enzyme replacement therapy (ERT) after 5 years and to identify predictors for a favorable response because few data are available on the long-term efficacy of ERT in Pompe disease. METHODS: We included 102 adult patients with Pompe disease in a nationwide, prospective cohort study. We assessed muscle strength (manual muscle testing with Medical Research Council [MRC] grading, handheld dynamometry [HHD]), muscle function (6-minute walk test, Quick Motor Function Test), daily life activities (Rasch-Built Pompe-Specific Activity [R-PAct] Scale), and pulmonary function (forced vital capacity [FVC] in upright and supine positions, maximum inspiratory and expiratory pressures) at 3- to 6-month intervals before and after the start of ERT...
November 8, 2017: Neurology
https://www.readbyqxmd.com/read/29112634/baroreflex-sensitivity-and-blood-pressure-variability-can-help-in-understanding-the-different-response-to-therapy-during-acute-phase-of-septic-shock
#18
Marta Carrara, Bernardo Bollen Pinto, Giuseppe Baselli, Karim Bendjelid, Manuela Ferrario
BACKGROUND: Mean values of hemodynamic variables are poorly effective in evaluating an actual recovery of the short-term autonomic mechanisms for blood pressure (BP) and heart rate (HR) regulation. The aim of this work is to analyze the responses to therapy in the early phase of septic shock in order to verify possible associations between BP recovery and BP autonomic control. METHODS: This is an ancillary study from the multicenter prospective observational trial Shockomics (NCT02141607)...
November 6, 2017: Shock
https://www.readbyqxmd.com/read/29110167/assessment-of-autonomic-nervous-system-modulation-after-novel-catheter-ablation-techniques-for-atrial-fibrillation-using-multiple-short-term-electrocardiogram-recordings
#19
Satoshi Yanagisawa, Yasuya Inden, Aya Fujii, Yosuke Kamikubo, Yasunori Kanzaki, Monami Ando, Junya Funabiki, Yosuke Murase, Masaki Takenaka, Noriaki Otake, Yoshihiro Ikai, Yusuke Sakamoto, Rei Shibata, Toyoaki Murohara
PURPOSE: The effect of novel catheter ablation techniques for atrial fibrillation (AF) on the autonomic nervous system (ANS) is unclear. This study aimed to assess the ANS after three novel catheter ablation techniques for paroxysmal AF by evaluating heart rate variability (HRV) parameters using a 3-min electrocardiogram recording. METHODS: Two hundred and thirty-five patients who underwent catheter ablation for paroxysmal AF (119 in irrigated-tip, 51 in contact-force sensing-guided, and 65 patients in second-generation cryoballoon ablation) were included...
November 6, 2017: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
https://www.readbyqxmd.com/read/29107952/hereditary-angioedema-assessing-the-hypothesis-for-underlying-autonomic-dysfunction
#20
Maddalena A Wu, Francesco Casella, Francesca Perego, Chiara Suffritti, Nada Afifi Afifi, Eleonora Tobaldini, Andrea Zanichelli, Chiara Cogliati, Nicola Montano, Marco Cicardi
BACKGROUND: Attacks of Hereditary Angioedema due to C1-inhibitor deficiency (C1-INH-HAE)are often triggered by stressful events/hormonal changes. OBJECTIVE: Our study evaluates the relationship between autonomic nervous system (ANS) and contact/complement system activation. METHODS: Twenty-three HAE patients (6 males, mean age 47.5±11.4 years) during remission and 24 healthy controls (8 males, mean age 45.3±10.6 years) were studied. ECG, beat-by-beat blood pressure, respiratory activity were continuously recorded during rest (10') and 75-degrees-head-up tilt (10')...
2017: PloS One
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