keyword
MENU ▼
Read by QxMD icon Read
search

spermine

keyword
https://www.readbyqxmd.com/read/28076812/spermine-condenses-dna-but-not-rna-duplexes
#1
Andrea M Katz, Igor S Tolokh, Suzette A Pabit, Nathan Baker, Alexey V Onufriev, Lois Pollack
Interactions between the polyamine spermine and nucleic acids drive important cellular processes. Spermine condenses DNA and some RNAs, such as poly(rA):poly(rU). A large fraction of the spermine present in cells is bound to RNA but apparently does not condense it. Here, we study the effect of spermine binding to short duplex RNA and DNA, and compare our findings with predictions of molecular-dynamics simulations. When small numbers of spermine are introduced, RNA with a designed sequence containing a mixture of 14 GC pairs and 11 AU pairs resists condensation relative to DNA of an equivalent sequence or to 25 bp poly(rA):poly(rU) RNA...
January 10, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28070313/decarboxylase-positive-enterococcus-faecium-strains-isolated-from-rabbit-meat-and-their-sensitivity-to-enterocins
#2
Andrea Lauková, Renata Szabóová, Pavel Pleva, Leona Buňková, Ľubica Chrastinová
BACKGROUND: The objective of the study was to determine sensitivity of Enterococcus faecium strains from rabbit meat to enterocins. RESULTS: Twenty-five decarboxylase-positive strains (from rabbit meat) allotted to the species E. faecium by genotypization and by MALDI TOF MS spectrometry identification (evaluation score value range 2.104-2.359; in the range for highly probable species identification-score value 2.300-3.000 and secure probable species identification/probable species identification-2...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28052993/spermine-and-spermidine-alter-gene-expression-and-antigenic-profile-of-borrelia-burgdorferi
#3
Ying-Han Lin, Jesus A Romo, Trever C Smith, Ann N Reyes, S L Rajasekhar Karna, Christine L Miller, Tricia A Van Laar, Raghunandan Yendapally, James P Chambers, J Seshu
Borrelia burgdorferi, the agent of Lyme disease, responds to numerous host-derived signals to alter adaptive capabilities during its enzootic cycle in an arthropod vector and mammalian host. Molecular mechanisms that enable B. burgdorferi to detect, channel, and respond to these signals have become an intense area of study for developing strategies to limit transmission/infection. Bioinformatic analysis of the borrelial genome revealed the presence of polyamine transport components (PotA, B, C and D) while homologs for polyamine biosynthesis were conspicuously absent...
January 4, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28052719/comparison-of-soybean-cultivars-for-enhancement-of-the-polyamine-contents-in-the-fermented-soybean-natto-using-bacillus-subtilis-natto
#4
Kazuya Kobayashi, Yuichiro Horii, Satoshi Watanabe, Yuji Kubo, Kumiko Koguchi, Yoshihiro Hoshi, Ken-Ichi Matsumoto, Kuniyasu Soda
Polyamines have beneficial properties to prevent aging-associated diseases. Raw soybean has relatively high polyamine contents; and the fermented soybean natto is a good source of polyamines. However, detailed information of diversity of polyamine content in raw soybean is lacking. The objectives of this study were to evaluate differences of polyamines among raw soybeans and select the high polyamine-containing cultivar for natto production. Polyamine contents were measured chromatographically in 16 samples of soybean, which showed high variation among soybeans as follows: 93-861 nmol/g putrescine, 1055-2306 nmol/g spermidine, and 177-578 nmol/g spermine...
January 5, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28043891/adaptive-responses-of-heart-and-skeletal-muscle-to-spermine-oxidase-overexpression-evaluation-of-a-new-transgenic-mouse-model
#5
Roberta Ceci, Guglielmo Duranti, Alessia Leonetti, Stefano Pietropaoli, Federico Spinozzi, Lucia Marcocci, Roberto Amendola, Francesco Cecconi, Stefania Sabatini, Paolo Mariottini, Manuela Cervelli
Spermine oxidase oxidizes spermine to produce H2O2, spermidine, and 3-aminopropanal. It is involved in cell drug response, apoptosis, and in the etiology of several pathologies, including cancer. Spermine oxidase is an important positive regulator of muscle gene expression and fiber size and, when repressed, leads to muscle atrophy. We have generated a transgenic mouse line overexpressing Smox gene in all organs, named Total-Smox. The spermine oxidase overexpression was revealed by β-Gal staining and reverse-transcriptase/PCR analysis, in all tissues analysed...
December 30, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28039518/polyamine-catabolism-adds-fuel-to-leaf-senescence
#6
REVIEW
E Sobieszczuk-Nowicka
Leaf senescence is a terminal step in plant growth and development. Considerable information on processes and signals involved in this process has been obtained, although comparatively little is known about leaf senescence in monocotyledonous plants. In particular, little is known about players involved in leaf senescence imposed by a prolonged dark treatment. New information has now been unveiled on dark-induced leaf senescence in a monocot, barley. A close association has been found between ubiquitous polyamines, reactive oxygen species (ROS), and senescence of barley leaves during prolonged darkness...
January 2017: Amino Acids
https://www.readbyqxmd.com/read/28030815/a-novel-non-canonical-wnt-signature-for-prostate-cancer-aggressiveness
#7
Elise Sandsmark, Ailin Falkmo Hansen, Kirsten M Selnæs, Helena Bertilsson, Anna M Bofin, Alan J Wright, Trond Viset, Elin Richardsen, Finn Drabløs, Tone F Bathen, May-Britt Tessem, Morten B Rye
Activation of the Canonical Wnt pathway (CWP) has been linked to advanced and metastatic prostate cancer, whereas the Wnt5a-induced non-canonical Wnt pathway (NCWP) has been associated with both good and poor prognosis. A newly discovered NCWP, Wnt5/Fzd2, has been shown to induce epithelial-to-mesenchymal transition (EMT) in cancers, but has not been investigated in prostate cancer. The aim of this study was to investigate if the CWP and NCWP, in combination with EMT, are associated with metabolic alterations, aggressive disease and biochemical recurrence in prostate cancer...
December 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/28008251/coating-with-spermine-pullulan-polymer-enhances-adenoviral-transduction-of-mesenchymal-stem-cells
#8
Li Wan, Xinglei Yao, Francesco Faiola, Bojun Liu, Tianyuan Zhang, Yasuhiko Tabata, Hiroyuki Mizuguchi, Shinsaku Nakagawa, Jian-Qing Gao, Robert Chunhua Zhao
Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27994754/polyamine-conjugation-as-a-promising-strategy-to-target-amyloid-aggregation-in-the-framework-of-alzheimer-s-disease
#9
Elena Simoni, Roberta Caporaso, Christian Bergamini, Jessica Fiori, Romana Fato, Przemyslaw Miszta, Sławomir Filipek, Filippo Caraci, Maria Laura Giuffrida, Vincenza Andrisano, Anna Minarini, Manuela Bartolini, Michela Rosini
Spermine conjugates 2-6, carrying variously decorated 3,5-dibenzylidenepiperidin-4-one as bioactive motives, were designed to direct antiaggregating properties into mitochondria, using a polyamine functionality as the vehicle tool. The study confirmed mitochondrial import of the catechol derivative 2, which displayed effective antiaggregating activity and neuroprotective effects against Aβ-induced toxicity. Notably, a key functional role for the polyamine motif in Aβ molecular recognition was also unraveled...
December 8, 2016: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27982735/differential-scanning-calorimetric-study-of-antibiotic-distamycin-a-binding-with-chromatin-within-isolated-rat-liver-nuclei
#10
Andrey N Prusov, Galina Ya Kolomijtseva, Tatiana A Smirnova
CONTEXT: Natural oligopeptide antibiotic distamycin A (Dst) biosynthesized by Streptomyces distallicus is traditionally used in medical practice as an anti-inflammatory and antitumour drug. OBJECTIVE: Dst was investigated for its effect on the structural components of native chromatin directly within isolated rat liver nuclei in the presence of physiologically significant cations (magnesium or spermine and spermidine). MATERIALS AND METHODS: Differential scanning calorimetry (DSC) was used to study the Dst action at molar ratio Dst/DNA = 0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27968713/a-new-multiresponse-optimization-approach-in-combination-with-a-d-optimal-experimental-design-for-the-determination-of-biogenic-amines-in-fish-by-hplc-fld
#11
A Herrero, S Sanllorente, C Reguera, M C Ortiz, L A Sarabia
A new strategy to approach multiresponse optimization in conjunction to a D-optimal design for simultaneously optimizing a large number of experimental factors is proposed. The procedure is applied to the determination of biogenic amines (histamine, putrescine, cadaverine, tyramine, tryptamine, 2-phenylethylamine, spermine and spermidine) in swordfish by HPLC-FLD after extraction with an acid and subsequent derivatization with dansyl chloride. Firstly, the extraction from a solid matrix and the derivatization of the extract are optimized...
November 16, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27943481/synchronized-chiral-induction-between-5-helicene-spermine-ligand-and-b-z-dna-transition
#12
Kensuke Kawara, Genichiro Tsuji, Yosuke Taniguchi, Shigeki Sasaki
The 2,13-dimethoxy[5]helicene-spermine ligand 8 b possesses an axial chirality. The racemic 8 b was bound to B-DNA by the accompanying induction of its (P)-chirality together with the B-to-Z helicity change of the duplex DNA, [(dC-dG)3 ]2 . The (P)-chirality of the bound 8 b, in turn, transitioned to the (M)-chirality according to the Z-helicity of the DNA. These results illustrate the chirality synchronization between the DNA and the ligand.
December 11, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27940184/sirna-mediated-in-vivo-gene-knockdown-by-acid-degradable-cationic-nanohydrogel-particles
#13
Nadine Leber, Leonard Kaps, Misbah Aslam, Jonathan Schupp, Alexander Brose, David Schäffel, Karl Fischer, Mustafa Diken, Dennis Strand, Kaloian Koynov, Andrea Tuettenberg, Lutz Nuhn, Rudolf Zentel, Detlef Schuppan
Cationic nanohydrogel particles have become an attractive tool for systemic siRNA delivery, but improvement of their in vivo tolerance is desirable, especially to prevent potential long term side effects by tissue and cellular accumulation. Here we designed novel ketal cross-linked cationic nanohydrogel particles that were assessed for reduced tissue accumulation and robust siRNA delivery in vitro and in vivo. An oligo-amine cross-linker equipped with a ketal moiety in its core was synthesized and applied to nanohydrogel cross-linking of self-assembled reactive ester block copolymers in DMSO...
December 8, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27916902/synthesis-and-biological-evaluation-of-novel-aromatic-imide-polyamine-conjugates
#14
Ming Li, Yuxia Wang, Jianying Zhang, Songqiang Xie, Chaojie Wang, Yingliang Wu
Three types of conjugates in which aromatic imide scaffolds were coupled to diverse amine/polyamine motifs were synthesized, and their antitumor activities were evaluated in vitro and in vivo. Results showed that the conjugate 11e of 1,8-naphthilimide with spermine had pronounced effects on inhibiting tumor cell proliferation and inducing tumor cell apoptosis via ROS-mediated mitochondrial pathway. The in vivo assays on three H22 tumor transplant models revealed that compound 11e exerted potent ability in preventing lung cancer metastasis and extending lifespan...
November 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27901475/spermidine-spermine-n1-acetyltransferase-regulates-cell-growth-and-metastasis-via-akt-%C3%AE-catenin-signaling-pathways-in-hepatocellular-and-colorectal-carcinoma-cells
#15
Cong Wang, Ping Ruan, Ying Zhao, Xiaomin Li, Jun Wang, Xiaoxiao Wu, Tong Liu, Shasha Wang, Jiuzhou Hou, Wei Li, Qian Li, Jinghua Li, Fujun Dai, Dong Fang, Chaojie Wang, Songqiang Xie
Hepatocellular carcinoma (HCC) and colorectal cancer (CRC) are among the most common cancers across the world. Therefore, identifying the potential molecular mechanisms that promote HCC and CRC progression and metastasis are urgently needed. Spermidine/spermine N1-acetyltransferase (SSAT) is a catabolic enzyme that acetylates the high-order polyamines spermine and spermidine, thus decreasing the cellular content of polyamines. Several publications have suggested that depletion of intracellular polyamines inhibited tumor progression and metastasis in various cancer cells...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27901061/inter-individual-variability-and-modeling-of-electrical-activity-a-possible-new-approach-to-explore-cardiac-safety
#16
Jean-Yves Le Guennec, Jérôme Thireau, Aude Ouillé, Julien Roussel, Jérôme Roy, Serge Richard, Sylvain Richard, Eric Martel, Pascal Champéroux
Safety pharmacology aims to predict rare side effects of new drugs. We explored whether rare pro-arrhythmic effects could be linked to the variability of the effects of these drugs on ion currents and whether taking into consideration this variability in computational models could help to better detect and predict cardiac side effects. For this purpose, we evaluated how intra- and inter-individual variability influences the effect of hERG inhibition on both the action potential duration and the occurrence of arrhythmias...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27885170/elucidation-of-the-polyamine-biosynthesis-pathway-during-brazilian-pine-araucaria-angustifolia-seed-development
#17
Leandro F de Oliveira, Paula Elbl, Bruno V Navarro, Amanda F Macedo, André L W Dos Santos, Eny I S Floh
Polyamines (PAs), such as spermidine and spermine, as well as amino acids that are substrates for their biosynthesis, are known to be essential for plant development. However, little is known about the gene expression and metabolic switches associated with the ornithine/arginine and PA biosynthetic pathway during seed development in conifers. To understand these metabolic switches, the enzyme activity of arginine decarboxylase and ornithine decarboxylase, as well as the contents of PAs and amino acids were evaluated in three Araucaria angustifolia (Bertol...
November 23, 2016: Tree Physiology
https://www.readbyqxmd.com/read/27880824/anti-invasive-and-anti-proliferative-synergism-between-docetaxel-and-a-polynuclear-pd-spermine-agent
#18
Ana L M Batista de Carvalho, Paula S C Medeiros, Francisco M Costa, Vanessa P Ribeiro, Joana B Sousa, Carmen Diniz, Maria P M Marques
The present work is aimed at evaluating the antitumour properties of a Pd(II) dinuclear complex with the biogenic polyamine spermine, by investigating: i) the anti-angiogenic and anti-migration properties of a Pd(II) dinuclear complex with spermine (Pd2Spm); ii) the anti-proliferative activity of Pd2Spm against a triple negative human breast carcinoma (MDA-MB-231); and finally iii) the putative interaction mediated by combination of Pd2Spm with Docetaxel. Anti-invasive (anti-angiogenic and anti-migratory) as well as anti-proliferative capacities were assessed, for different combination schemes and drug exposure times, using the CAM assay and VEGFR2 activity measurement, the MatrigelTM method and the SRB proliferation test...
2016: PloS One
https://www.readbyqxmd.com/read/27877214/role-of-urinary-biomarkers-in-the-diagnosis-of-adenoma-and-colorectal-cancer-a-systematic-review-and-meta-analysis
#19
REVIEW
Emma Altobelli, Paolo Matteo Angeletti, Giovanni Latella
The growing interest in enhancing and spreading colorectal cancer (CRC) screening has been stimulating the exploration of novel biomarkers with greater sensitivity and specificity than immunochemical faecal occult blood test (iFOBT). The present study provides i) a systematic review of the urinary biomarkers that have been tested to achieve early CRC diagnosis and assess the risk of colorectal adenoma and adenocarcinoma, and ii) a meta-analysis of the data regarding the urinary prostaglandin (PG) metabolite PGE-M...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/27876565/magnesium-chloride-and-polyamine-can-differentiate-mouse-embryonic-stem-cells-into-trophectoderm-or-endoderm
#20
Jun-Ichi Tanase, Takehiro Yokoo, Yuuki Matsumura, Makoto Kinoshita, Yo Kikuchi, Hirofumi Suemori, Takashi Ohyama
Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensation in vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm...
January 22, 2017: Biochemical and Biophysical Research Communications
keyword
keyword
76600
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"