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https://www.readbyqxmd.com/read/28733445/enhanced-no-dependent-pulmonary-vasodilation-limits-increased-vasoconstrictor-sensitivity-in-neonatal-chronic-hypoxia
#1
Joshua R Sheak, Laura Weise-Cross, Ray J deKay, Benjimen R Walker, Nikki L Jernigan, Thomas C Resta
Augmented vasoconstrictor reactivity is thought to play an important role in the development of chronic hypoxia (CH)-induced neonatal pulmonary hypertension (pHTN). However, whether this response to CH results from pulmonary endothelial dysfunction and reduced NO-mediated vasodilation is not well understood. We hypothesized that neonatal CH enhances basal tone and pulmonary vasoconstrictor sensitivity by limiting NO-dependent pulmonary vasodilation. To test this hypothesis, we assessed effects of the NO synthase (NOS) inhibitor N(w)-nitro-L-arginine (L-NNA) on baseline pulmonary vascular resistance (PVR) and vasoconstrictor sensitivity to the thromboxane mimetic U-46619 in saline-perfused lungs (in situ) from 2-wk-old control and CH (12 day exposure, 0...
July 21, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28732914/spermine-modified-starch-based-carrier-for-gene-delivery-structure-transfection-activity-relationships
#2
Xiaoyi Huang, Xiaoxi Li, Ling Chen, Lin Li
This study aims at developing starch based gene carriers with low cytotoxicity and high transfection efficiency. Starch molecules with molecular weight of about 50kDa were cationically modified by spermine to obtain spermine modified starch (SMS) based gene carriers. Plasmid pAcGFP1-C1 (pDNA) was chosen as the model gene material and formed self-assembly nanocomplexes with SMS. The cytotoxicity and transfection efficiency of SMS/pDNA complexes were tested in HepG2 cell lines. Results showed that SMS/pDNA complexes formed by SMS-DS3 with the highest primary amine content (1...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28727033/the-effect-of-age-and-carbohydrate-and-protein-sources-on-digestibility-fecal-microbiota-fermentation-products-fecal-iga-and-immunological-blood-parameters-in-dogs
#3
A P J Maria, L Ayane, T C Putarov, B A Loureiro, B P Neto, M F Casagrande, M O S Gomes, M B A Glória, A C Carciofi
The present study compared the effects of diets formulated with fibers of different fermentability and protein sources of animal or vegetable origins on old and adult dogs. The experiment was organized in a 3 (diets) × 2 (ages) factorial arrangement, totaling 6 treatments. Thirty-six Beagle dogs were used (18 old dogs [10.2 ± 1.0 yr] and 18 young adult dogs [2.6 ± 0.9 yr]), with 6 dogs per treatment. Three diets with similar compositions were used: a nonfermentable insoluble fiber source (sugarcane fiber) and chicken byproduct meal (nonfermentable fiber [NFF] diet), a fermentable fiber source (beet pulp) and chicken byproduct meal (fermentable fiber [FF] diet), and soybean meal as a protein and fiber source (soybean meal [SM] diet)...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28707668/-low-molecular-weight-regulators-of-biogenic-polyamine-metabolism-affect-cytokine-production-and-expression-of-hepatitis-%C3%B0-virus-proteins-in-huh7-5-human-hepatocarcinoma-cells
#4
O V Masalova, E I Lesnova, E I Samokhvalov, K Yu Permyakova, A V Ivanov, S N Kochetkov, A A Kushch
Hepatitis C virus (HCV) induces the expression of the genes of proinflammatory cytokines, the excessive production of which may cause cell death, and contribute to development of liver fibrosis and hepatocarcinoma. The relationship between cytokine production and metabolic disorders in HCV-infected cells remains obscure. The levels of biogenic polyamines, spermine, spermidine, and their precursor putrescine, may be a potential regulator of these processes. The purpose of the present work was to study the effects of the compounds which modulate biogenic polyamines metabolism on cytokine production and HCV proteins expression...
May 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28699025/polyamines-increase-nitric-oxide-and-reactive-oxygen-species-in-guard-cells-of-arabidopsis-thaliana-during-stomatal-closure
#5
Srinivas Agurla, Gunja Gayatri, Agepati S Raghavendra
A comprehensive study which was undertaken on the effect of three polyamines (PAs) on stomatal closure was examined in relation to nitric oxide (NO) and reactive oxygen species (ROS) levels in guard cells of Arabidopsis thaliana. Three PAs-putrescine (Put), spermidine (Spd), and spermine (Spm)-induced stomatal closure, while increasing the levels of NO as well as ROS in guard cells. The roles of NO and ROS were confirmed by the reversal of closure by cPTIO (NO scavenger) and catalase (ROS scavenger). The presence of L-NAME (NOS-like enzyme inhibitor) reversed PA-induced stomatal closure, suggesting that NOS-like enzyme played a significant role in NO production during stomatal closure...
July 11, 2017: Protoplasma
https://www.readbyqxmd.com/read/28694504/melatonin-alleviates-chilling-stress-in-cucumber-seedlings-by-up-regulation-of-cszat12-and-modulation-of-polyamine-and-abscisic-acid-metabolism
#6
Hailiang Zhao, Kai Zhang, Xiaoting Zhou, Linjie Xi, Yuping Wang, Hongjun Xu, Tonghua Pan, Zhirong Zou
To obtain new insights into the mechanisms of the positive effects of exogenous melatonin applications to cucumber seedlings during chilling, we investigated its role in regulating photosynthesis, the transcription level of csZat12 and the metabolism of polyamines (PAs) and of abscisic acid (ABA). The negative effects of chilling were clearly alleviated in cucumber seedlings by irrigation with 200 μM melatonin solution. This was evidenced by alleviation of the decline in net photosynthesis rate and also in electrolyte leakage in chilled plants...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28677399/super-cationic-carbon-quantum-dots-synthesized-from-spermidine-as-an-eye-drop-formulation-for-topical-treatment-of-bacterial-keratitis
#7
Hong-Jyuan Jian, Ren-Siang Wu, Tzu-Yu Lin, Yu-Jia Li, Han-Jia Lin, Scott G Harroun, Jui-Yang Lai, Chih-Ching Huang
We have developed a one-step method to synthesize carbon quantum dots (CQDPAs) from biogenic polyamines (PAs) as an antibacterial agent for topical treatment of bacterial keratitis (BK). CQDs synthesized by direct pyrolysis of spermidine (Spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. We demonstrate that CQDs obtained from Spds (CQDSpds) possess effective antibacterial activities against non-multidrug-resistant Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica serovar Enteritidis bacteria and also against the multidrug-resistant bacteria, methicillin-resistant S...
July 5, 2017: ACS Nano
https://www.readbyqxmd.com/read/28672910/pre-treatment-with-spermine-for-acute-cerebral-ischemia-reperfusion-injuries
#8
Hai Du, Xing Ming, Shengkui Zhou
Acute cerebral ischemia/reperfusion injury (ACIR) rat model was used to investigate the effect of spermine on oxidative stress and apoptosis. Sixty healthy and clean male Sprague-Dawley rats were randomly divided into 3 groups: The sham-operated group (n=12), the model group (n=12) and the experiment group (n=36). The experiment group was further divided into 3 subgroups: The SPE-10 group (n=12), the SPE-25 group (n=12) and the SPE-50 group (n=12). Rats in the experimental sub-groups SPE-10, SPE-25 and SPE-50 were injected with 10, 25 and 50 mg/kg of spermine, respectively, one week before the establishment of rat models...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28667385/potential-application-of-sers-for-arsenic-speciation-in-biological-matrices
#9
Mingwei Yang, Shannon Matulis, Lawrence H Boise, Anthony J McGoron, Yong Cai
Speciation of arsenic is usually carried out using chromatography-based methods coupled with spectroscopic determination; however, the inevitable procedures involving sample preparation and separation could potentially alter the integrity of the arsenic metabolites present in biological samples. Surface-enhanced Raman spectroscopy (SERS) could be a promising alternative for providing a reliable arsenic analysis under the influence of a cellular matrix. A method for arsenic speciation using SERS in cellular matrix was developed in this study and four arsenicals were selected, including arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MMA(V)) and dimethylarsinic acid (DMA(V))...
June 30, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28659731/modulatory-effect-of-intestinal-polyamines-and-trace-amines-on-the-spontaneous-phasic-contractions-of-the-isolated-ileum-and-colon-rings-of-mice
#10
Manuel Sánchez, Lorena Suárez, María Teresa Andrés, Blanca Henar Flórez, Javier Bordallo, Sabino Riestra, Begoña Cantabrana
Background: Gastrointestinal motility modulatory factors include substances of the intestinal content, such as polyamines and trace amines (TAs), the focus of this study. Methods: The amines of food, intestinal content and from faecal bacteria of Swiss mice were determined by HPLC and functionally characterised in isolated distal ileum and medial colon rings. Results: Mouse food and intestinal content contain polyamines (spermidine>putrescine>spermine) and TAs (isoamylamine>cadaverine). Intestinal bacteria mainly produce putrescine and cadaverine...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28650039/stabilization-vs-destabilization-of-g-quadruplex-superstructures-the-role-of-the-porphyrin-derivative-having-spermine-arms
#11
A D'Urso, R Randazzo, V Rizzo, C M A Gangemi, V Romanucci, A Zarrelli, G Tomaselli, D Milardi, N Borbone, R Purrello, G Piccialli, G Di Fabio, G Oliviero
The interaction of the porphyrin derivative H2TCPPSpm4, having spermine pendants in the four meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG has been investigated by means of UV, electronic circular dichroism and PAGE studies. The results reported here demonstrate that the porphyrin derivative is capable of stabilizing or destabilizing the higher-ordered structures of parallel GQs, depending on the method used to reach their relative stoichiometry (titration vs. single addition)...
June 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28641150/polyamine-and-tyramine-involvement-in-nacl-induced-improvement-of-cd-resistance-in-the-halophyte-inula-chrithmoides-l
#12
Rim Ghabriche, Tahar Ghnaya, Mejda Mnasri, Hanen Zaier, Raoudha Baioui, Delphine Vromman, Chedly Abdelly, Stanley Lutts
The aim of the present work was to analyze the impact of salinity on the plant response to Cd toxicity in the Mediterranean halophyte species Inula crithmoides. For this purpose, cuttings were cultivated hydroponically during 21d in the presence of 0, 25 or 50μM CdCl2 combined or not with 0, 100, 200 and 400mM NaCl. The obtained data demonstrated that, in the absence of Cd, NaCl strongly increased plant growth (the maximal dry weight being observed at 100mM) and enhanced the Na(+)/K(+) ratio in the shoot. Cd alone strongly affected plant growth in this halophyte...
June 6, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28639394/transferrin-conjugated-magnetic-dextran-spermine-nanoparticles-for-targeted-drug-transport-across-blood-brain-barrier
#13
Maryam Ghadiri, Ebrahim Vasheghani-Farahani, Fatemeh Atyabi, Farzad Kobarfard, Farzaneh Mohamadyar-Toupkanlou, Hossein Hosseinkhani
Application of many vital hydrophilic medicines have been restricted by blood-brain barrier (BBB) for treatment of brain diseases. In this study, a targeted drug delivery system based on dextran-spermine biopolymer was developed for drug transport across BBB. Drug loaded magnetic dextran-spermine nanoparticles (DS-NPs) were prepared via ionic gelation followed by transferrin (Tf) conjugation as targeting moiety. The characteristics of Tf conjugated nanoparticles (TDS-NPs) were analyzed by different methods and their cytotoxicity effects on U87MG cells were tested...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28633130/synthesis-screening-and-pro-apoptotic-activity-of-novel-acyl-spermidine-derivatives-on-human-cancer-cell-lines
#14
Syed Shoeb Razvi, Hani Choudhry, Said Salama Moselhy, Taha Abduallah Kumosani, Mohammed Nihal Hasan, Mazin A Zamzami, Khalid Omer Abualnaja, Abdulrahman Labeed Al-Malki, Mahmoud Alhosin, Tadao Asami
The polyamines putrescine, spermidine, and spermine are polycationic, alkyl polyamines which play a significant role in eukaryotic cell proliferation. The polyamine metabolism and function are dysregulated in tumor cells making them an attractive therapeutic target by employing polyamine analogs. These analogs have a high degree of similarity with the structure of polyamines but not with their function. Multidrug resistance is a major factor in the failure of many chemotherapeutic drugs which necessitates further research and exploration of better novel alternatives...
June 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28632118/tessaracoccus-arenae-sp-nov-isolated-from-sea-sand
#15
Chutimon Thongphrom, Jong-Hwa Kim, Nagamani Bora, Wonyong Kim
A Gram-stain positive, non-spore-forming, non-motile, facultatively anaerobic bacterial strain, designated CAU 1319T, was isolated from sea sand and the strain's taxonomic position was investigated using a polyphasic approach. Strain CAU 1319T grew optimally at 30 °C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain CAU 1319T belongs to the genus Tessaracoccus, and is closely related to Tessaracoccus lapidicaptus IPBSL-7T (similarity 97...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28624871/synthesis-and-application-of-magnetite-dextran-spermine-nanoparticles-in-breast-cancer-hyperthermia
#16
Reza Avazzadeh, Ebrahim Vasheghani-Farahani, Masoud Soleimani, Saeid Amanpour, Mohsen Sadeghi
Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41-45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method...
June 17, 2017: Progress in biomaterials
https://www.readbyqxmd.com/read/28614056/spermine-and-spermidine-reversed-age-related-cardiac-deterioration-in-rats
#17
Hao Zhang, Junying Wang, Lingxu Li, Nannan Chai, Yuhan Chen, Feixiang Wu, Weihua Zhang, Lina Wang, Sa Shi, Li Zhang, Shuling Bian, Changqing Xu, Ye Tian, Yajun Zhao
Aging is the most important risk factor for cardiovascular disease (CVD). Slowing or reversing the physiological impact of heart aging may reduce morbidity and mortality associated with age-related CVD. The polyamines, spermine (SP) and spermidine (SPD) are essential for cell growth, differentiation and apoptosis, and levels of both decline with age. To explore the effects of these polyamines on heart aging, we administered SP or SPD intraperitoneally to 22- to 24-month-old rats for 6 weeks. Both treatments reversed and inhibited age-related myocardial morphology alterations, myocardial fibrosis, and cell apoptosis...
May 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28603015/activation-of-a-ca-2-dependent-cation-conductance-with-properties-of-trpm2-by-reactive-oxygen-species-in-lens-epithelial-cells
#18
Susanne Keckeis, Laura Wernecke, Daniel J Salchow, Nadine Reichhart, Olaf Strauß
Ion channels are crucial for maintenance of ion homeostasis and transparency of the lens. The lens epithelium is the metabolically and electrophysiologically active cell type providing nutrients, ions and water to the lens fiber cells. Ca(2+)-dependent non-selective ion channels seem to play an important role for ion homeostasis. The aim of the study was to identify and characterize Ca(2+)- and reactive oxygen species (ROS)-dependent non-selective cation channels in human lens epithelial cells. RT-PCR revealed gene expression of the Ca(2+)-activated non-selective cation channels TRPC3, TRPM2, TRPM4 and Ano6 in both primary lens epithelial cells and the cell line HLE-B3, whereas TRPM5 mRNA was only found in HLE-B3 cells...
June 8, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28588603/short-term-effect-of-salt-shock-on-ethylene-and-polyamines-depends-on-plant-salt-sensitivity
#19
Pedro J Zapata, María Serrano, Manuel F García-Legaz, M T Pretel, M A Botella
In the present manuscript the short term effect (3-24 h) of a saline shock (NaCl 100 mM) on fresh weight, water content, respiration rate, ethylene production and Na(+), Cl(-), ACC and polyamine concentration was studied in four plant species with different salt sensitivity, pepper, lettuce, spinach, and beetroot. Higher reduction in fresh weight and water content as a consequence of saline shock was found in pepper and lettuce plants than in spinach and beetroot, the latter behaving as more salinity tolerant...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28587687/physiological-importance-of-polyamines
#20
Yasser Y Lenis, Mohammed A Elmetwally, Juan G Maldonado-Estrada, Fuller W Bazer
Polyamines are polycationic molecules that contain two or more amino groups (-NH3 +) and are present in all eukaryotic and prokaryotic cells. Polyamines are synthesized from arginine, ornithine, and proline, and from methionine as the methyl-group donor. In the traditional pathway for polyamine synthesis, arginase converts arginine into ornithine, which is decarboxylated by ornithine decarboxylase (ODC1) to generate putrescine. The latter is converted to spermidine and spermine. Recent studies have indicated the existence of 'non-classical pathways' for the generation of putrescine from arginine and proline in animal cells...
June 7, 2017: Zygote: the Biology of Gametes and Early Embryos
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