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https://www.readbyqxmd.com/read/28330220/involvement-of-exogenous-polyamines-enhances-regeneration-and-agrobacterium-mediated-genetic-transformation-in-half-seeds-of-soybean
#1
Muthukrishnan Arun, Arunachalam Chinnathambi, Kondeti Subramanyam, Sivabalan Karthik, Ganeshan Sivanandhan, Jeevaraj Theboral, Sulaiman Ali Alharbi, Chang Kil Kim, Andy Ganapathi
The present work demonstrates the participation of polyamines (PAs) to improve direct regeneration and Agrobacterium-mediated transformation in soybean half-seeds. The inclusion of PAs to culture medium along with optimal plant growth regulators (PGRs) enhanced shoot induction [98.3 %; 4.44 µM N(6)-benzyladenine (BA) and 103.27 µM spermidine] and elongation [90.0 %; 1.45 µM gibberellic acid (GA3) and 49.42 µM spermine]. The polyamine putrescine (62.08 µM) alone greatly enriched root induction (96...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324173/effect-of-oaz1-overexpression-on-goose-ovarian-granulosa-cells
#2
Bo Kang, Dongmei Jiang, Hui He, Rong Ma, Ziyu Chen, Zhixin Yi
Ornithine decarboxylase antizyme 1 (OAZ1) regulates both polyamine biosynthesis and transport and acts as a tumor suppressor because of its functions in the regulation of cell proliferation, apoptosis and growth. However, the roles of OAZ1 in the regulation of polyamine metabolism, cell proliferation, apoptosis and hormone receptor expression in granulosa cells (GCs) from geese have not been determined. To define the roles of OAZ1 in primary GCs, we constructed and transfected an Oaz1 overexpression vector with a thymidine deletion into primary GCs...
March 21, 2017: Amino Acids
https://www.readbyqxmd.com/read/28317753/bioactive-amines-and-phenolic-compounds-in-cocoa-beans-are-affected-by-fermentation
#3
Brenda de Nazaré do Carmo Brito, Renan Campos Chisté, Rosinelson da Silva Pena, Maria Beatriz Abreu Gloria, Alessandra Santos Lopes
Cocoa is the target of increased scientific research as it is one of the richest source of bioactive compounds. The formation of bioactive amines and their changes in cocoa beans during seven days of traditional fermentation was investigated for the first time. In addition, total phenolic compounds, anthocyanins contents and the scavenging capacity against ABTS radical were determined to monitor the fermentation process. Only two biogenic amines (tryptamine and tyramine) and two polyamines (spermidine and spermine) were detected in cocoa beans during fermentation...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28316010/protective-effect-of-lactobacillus-casei-on-dmh-induced-colon-carcinogenesis-in-mice
#4
Cesar Antonio Irecta-Nájera, María Del Rosario Huizar-López, Josefina Casas-Solís, Patricia Castro-Félix, Anne Santerre
The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation...
March 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28315656/expression-of-odc1-spd-spm-and-azin1-in-the-hypothalamus-ovary-and-uterus-during-rat-estrous-cycle
#5
Joseph R D Fernandes, Sammit Jain, Arnab Banerjee
The aim of the present study was to investigate variation in the expression pattern of ornithine decarboxylase (ODC1), spermine (SPM), spermidine (SPD) and antizyme inhibitor (AZIN1) in hypothalamus, ovary and uterus during the estrous cycle of rats. Further, to understand any correlation between polyamines and GnRH I expression in hypothalamus; effect of putrescine treatment on GnRH I expression in hypothalamus and progesterone and estradiol levels in serum were investigated. The study also aims in quantifying all the immunohistochemistry images obtained based on pixel counting algorithm to yield the relative pixel count...
March 15, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28302706/acclimatization-of-the-systemic-microcirculation-to-alveolar-hypoxia-is-mediated-by-inos-dependent-increased-nitric-oxide-availability
#6
Alfred J Casillan, Jie Chao, John G Wood, Norberto C Gonzalez
Rats breathing 10% O2 show a rapid and widespread systemic microvascular inflammation that results from nitric oxide (NO) depletion secondary to increased reactive O2 species (ROS) generation. The inflammation eventually resolves and the microcirculation becomes resistant to more severe hypoxia. These experiments were directed to determine the mechanisms underlying this microvascular acclimatization process. Intravital microscopy of the mesentery showed that after three weeks of hypoxia (PB ~380 Torr, inspired PO2 ~ 68-70 Torr) rats showed no evidence of inflammation; however, treatment with the inducible NO synthase (iNOS) inhibitor L-N(6)-(1-Iminoethyl) lysine dihydrochloride (L-NIL) led to ROS generation, leukocyte-endothelial adherence and emigration, and increased vascular permeability...
March 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28301837/breast-milk-polyamines-and-microbiota-interactions-impact-of-mode-of-delivery-and-geographical-location
#7
Carlos Gómez-Gallego, Himanshu Kumar, Izaskun García-Mantrana, Elloise du Toit, Jukka-Pekka Suomela, Kaisa M Linderborg, Yumei Zhang, Erika Isolauri, Baoru Yang, Seppo Salminen, M Carmen Collado
BACKGROUND/AIMS: The aim of the present study was to identify and quantify the polyamine levels in human milk obtained from different countries and through different modes of delivery, and to investigate their association with breast milk microbes. METHODS: Mature breast milk samples were obtained from 78 healthy mothers after 1 month of lactation from 4 different geographical locations: Finland, Spain (Europe); South Africa (Africa); and China (Asia). Polyamines were determined using HPLC after dansyl derivatization and milk microbiota was obtained by 16S rRNA gene sequencing...
March 17, 2017: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/28278250/zinc-reduces-epithelial-barrier-compromise-induced-by-human-seminal-plasma
#8
James M Mullin, Katherine M Diguilio, Mary C Valenzano, Rachael Deis, Sunil Thomas, E Peter Zurbach, Shaheed Abdulhaqq, Luis J Montaner
Human semen has the potential to modulate the epithelial mucosal tissues it contacts, as seminal plasma (SP) is recognized to contain both pro- and anti-barrier components, yet its effects on epithelial barrier function are largely unknown. We addressed the role of human SP when exposed to the basal-lateral epithelial surface, a situation that would occur clinically with prior mechanical or disease-related injury of the human epithelial mucosal cell layers in contact with semen. The action of SP on claudins-2, -4, -5, and -7 expression, as well as on a target epithelium whose basolateral surface has been made accessible to SP, showed upregulation of claudins-4 and -5 in CACO-2 human epithelial cell layers, despite broad variance in SP-induced modulation of transepithelial electrical resistance and mannitol permeability...
2017: PloS One
https://www.readbyqxmd.com/read/28272780/microfluidic-encapsulation-of-prickly-zinc-doped-copper-oxide-nanoparticles-with-vd1142-modified-spermine-acetalated-dextran-for-efficient-cancer-therapy
#9
Hongbo Zhang, Dongfei Liu, Liang Wang, Zehua Liu, Runrun Wu, Agne Janoniene, Ming Ma, Guoqing Pan, Lina Baranauskiene, Linlin Zhang, Wenguo Cui, Vilma Petrikaite, Daumantas Matulis, Hongxia Zhao, Jianming Pan, Hélder A Santos
Structural features of nanoparticles have recently been explored for different types of applications. To explore specific particles as nanomedicine and physically destroy cancer is interesting, which might avoid many obstacles in cancer treatment, for example, drug resistance. However, one key element and technical challenge of those systems is to selectively target them to cancer cells. As a proof-of-concept, Prickly zinc-doped copper oxide (Zn-CuO) nanoparticles (Prickly NPs) have been synthesized, and subsequently encapsulated in a pH-responsive polymer; and the surface has been modified with a novel synthesized ligand, 3-(cyclooctylamino)-2,5,6-trifluoro-4-[(2-hydroxyethyl)sulfonyl] benzenesulfonamide (VD1142)...
March 8, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28261254/interaction-of-polyamines-abscisic-acid-nitric-oxide-and-hydrogen-peroxide-under-chilling-stress-in-tomato-lycopersicon-esculentum-mill-seedlings
#10
Qiannan Diao, Yongjun Song, Dongmei Shi, Hongyan Qi
Polyamines (PAs) play a vital role in the responses of higher plants to abiotic stresses. However, only a limited number of studies have examined the interplay between PAs and signal molecules. The aim of this study was to elucidate the cross-talk among PAs, abscisic acid (ABA), nitric oxide (NO), and hydrogen peroxide (H2O2) under chilling stress conditions using tomato seedlings [(Lycopersicon esculentum Mill.) cv. Moneymaker]. The study showed that during chilling stress (4°C; 0, 12, and 24 h), the application of spermidine (Spd) and spermine (Spm) elevated NO and H2O2 levels, enhanced nitrite reductase (NR), nitric oxide synthase (NOS)-like, and polyamine oxidase activities, and upregulated LeNR relative expression, but did not influence LeNOS1 expression...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28258224/transglutaminase-catalyzed-incorporation-of-polyamines-masks-the-dna-binding-region-of-the-transcription-factor-relish
#11
Kouki Maki, Toshio Shibata, Shun-Ichiro Kawabata
In Drosophila, the immune deficiency (IMD) pathway-dependent signal is finally transmitted through proteolytic conversion of the nuclear factor-κB-like transcription factor Relish to the active N-terminal fragment Relish-N to induce its translocation from the cytosol into the nucleus for the expression of IMD-controlled genes. We previously demonstrated that transglutaminase (TG) suppresses the IMD pathway by polymerizing Relish-N to inhibit its nuclear translocation. On the other hand, we also demonstrated that a synthetic amine, such as monodansylcadaverine (DCA) or biotin-labeled pentylamine, ingested by flies is TG-dependently incorporated into Relish-N, causing the nuclear translocation of modified Relish-N in gut epithelial cells...
March 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28254666/kukoamine-a-attenuates-insulin-resistance-and-fatty-liver-through-downregulation-of-srebp-1c
#12
Guangyun Li, Fang Zhou, Ying Chen, Weiguo Zhang, Ning Wang
Nonalcoholic fatty liver disease (NAFLD) refers to a pathological condition of hepatic steatosis. Insulin resistance is believed to be the key mechanism mediating initial accumulation of fat in the liver, resulting in hepatic steatosis. Kukoamine A (KuA), a spermine alkaloid, is a major bioactive component extracted from the root barks of Lycium chinense (L. chinense) Miller. In the current study, we aimed to explore the possible effect of KuA on insulin resistance and fatty liver. We showed that KuA significantly inhibited the increase of fasting blood glucose level and insulin level, and the glucose levels in response to glucose and insulin load in HFD-fed mice, which was in a dose-dependent manner...
February 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28254465/spermidine-improves-the-persistence-of-reconsolidated-fear-memory-and-neural-differentiation-in-vitro-involvement-of-bdnf
#13
Cristiane Signor, Bruna Amanda Girardi, Arithane Lorena Wendel, Pâmella Karina Santana Frühauf, Micheli M Pillat, Henning Ulrich, Carlos F Mello, Maribel A Rubin
Putrescine, spermidine and spermine are organic cations implicated in learning, memory consolidation, reconsolidation and neurogenesis. These physiological processes are closely related, and convincing evidence indicates that neurogenesis is implicated both, in the establishment and maintenance of remote contextual fear memory. Although brain-derived neurotrophic factor (BDNF) is a key mediator involved in both neurogenesis and memory consolidation, effects of spermidine on persistence of memory after reactivation (reconsolidation) and possible involvement of BDNF have not been investigated...
February 22, 2017: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/28250271/lead-induced-erk-activation-is-mediated-by-glur2-non-containing-ampa-receptor-in-cortical-neurons
#14
Keishi Ishida, Yaichiro Kotake, Seigo Sanoh, Shigeru Ohta
Lead is a persistent environmental pollutant and exposure to high environmental levels causes various deleterious toxicities, especially to the central nervous system (CNS). The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor that is devoid of the glutamate receptor 2 (GluR2) subunit is Ca(2+)-permeable, which increases the neuronal vulnerability to excitotoxicity. We have previously reported that long-term exposure of rat cortical neurons to lead acetate induces decrease of GluR2 expression...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28238850/further-studies-of-the-effects-of-aging-on-arginine-metabolites-in-the-rat-vestibular-nucleus-and-cerebellum
#15
P Liu, N Gupta, Y Jing, N D Collie, H Zhang, P F Smith
Some studies have demonstrated that aging is associated with impaired vestibular reflexes, especially otolithic reflexes, resulting in postural instability. However, the neurochemical basis of these age-related changes is still poorly understood. The l-arginine metabolic system has been implicated in changes in the brain associated with aging. In the current study, we examined the levels of l-arginine and its metabolizing enzymes and downstream metabolites in the vestibular nucleus complex (VNC) and cerebellum (CE) of rats with and without behavioral testing which were young (4months old), middle-aged (12months old) or aged (24months old)...
February 24, 2017: Neuroscience
https://www.readbyqxmd.com/read/28236685/conjugation-of-chlorins-with-spermine-enhances-phototoxicity-to-cancer-cells-in-vitro
#16
Mariia Darmostuk, Michal Jurášek, Kamila Lengyel, Jaroslav Zelenka, Michaela Rumlová, Pavel Drašar, Tomáš Ruml
Photodynamic therapy (PDT) is one of the most promising methods of specific cancer treatment. However, commercially available photosensitizers (PSs) show significant drawbacks, such as side toxicity, low penetration ability, low blood solubility, low tumor selectivity etc. In addition, as was shown previously, a conjugation of polyamines with several toxic agents led to an increased toxicity to cancer cells. Here, we synthesized conjugates of two chlorine photosensitizers, purpurin 18 and pheophorbide a, with spermine in natural and Boc-protected form...
February 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28221716/ionic-self-assembled-platform-of-perylenediimide-sodium-dodecylsulfate-for-detection-of-spermine-in-clinical-samples
#17
Prabhpreet Singh, Lalit Singh Mittal, Gaurav Bhargava, Subodh Kumar
The detection and quantification of spermine in clinical practice is important for early diagnosis of many diseases. The chromatographic and immunoassay-based methods are commonly used. However, fluorescence-based assay could provide real time detection. Herein, we report synthesis and aggregation properties of dicationic perylene probe (DAB-PDI) and used DAB-PDI in developing fluorescent 'turn-on' ensemble for detection of spermine. The fluorescence of DAB-PDI (10 µM, Φ = 0.55) is efficiently quenched by negatively charged sodium dodecylsulfate (SDS) through formation of ionic self-assembled aggregates (charge ratio N/P = 9)...
February 21, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28212867/protective-effect-of-spermine-against-pentylenetetrazole-kindling-epilepsy-induced-comorbidities-in-mice
#18
Mandeep Kumar, Puneet Kumar
Nitric oxide (NO), an important intracellular signaling molecule is involved in modulation of neuronal transmission. The NO level increases during epileptic activity in animal models of epilepsy. However, its role in epileptic activity remains controversial. Spermine is an endogenous polyamine; possesses anti-oxidant property and has ability to modulate ion channels and NO synthase activity. Therefore, the present study was designed to investigate the role of NO pathway in the neuroprotective effect of spermine, in Pentylenetetrazol (PTZ) induced kindling epilepsy in mice...
February 14, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28194936/supramolecular-chemotherapy-cooperative-enhancement-of-antitumor-activity-by-combining-controlled-release-of-oxaliplatin-and-consuming-of-spermine-by-cucurbit-7-uril
#19
Yueyue Chen, Zehuan Huang, Hanyang Zhao, Jiang-Fei Xu, Zhiwei Sun, Xi Zhang
Supramolecular chemotherapy is aimed to employ supramolecular approach for regulating the cytotoxicity and improving the efficiency of antitumor drugs. In this paper, we demonstrated a new example of supramolecular chemotherapy by utilizing the clinical antitumor drug, oxaliplatin, which is the specific drug for colorectal cancer treatment. Cytotoxicity of oxaliplatin to the colorectal normal cell could be significantly decreased by host-guest complexation between oxaliplatin and cucurbit[7]uril (CB[7]). More importantly, oxaliplatin-CB[7] exhibited cooperatively enhanced antitumor activity than oxaliplatin itself...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28178573/enhancement-of-cold-and-salt-tolerance-of-arabidopsis-by-transgenic-expression-of-the-s-adenosylmethionine-decarboxylase-gene-from-leymus-chinensis
#20
Zhujiang Liu, Panpan Liu, Dongmei Qi, Xianjun Peng, Gongshe Liu
Leymus chinensis is an important perennial forage grass natively distributed in the Eurasian Steppe. However, little is known about the molecular mechanism of its adaptation to extreme environmental conditions. Based on L. chinensis cold-treated sequence database, a highly expressed S-adenosylmethionine decarboxylase gene (LcSAMDC1) was isolated from L. chinensis. Gene structure analysis showed that LcSAMDC1 has two introns and three exons as well as three non-overlapping ORFs in its mRNA sequence. One hour of cold exposure caused a significant up-regulation of LcSAMDC1, while abscisic acid (ABA), salt, and osmotic stresses slightly induced its expression...
April 2017: Journal of Plant Physiology
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