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https://www.readbyqxmd.com/read/29339152/biogenic-triamine-and-tetraamine-activate-core-catalytic-ability-of-tetrahymena-group-i-ribozyme-in-the-absence-of-its-large-activator-module
#1
Mst Ara Gulshan, Md Motiar Rahman, Shigeyoshi Matsumura, Tsunehiko Higuchi, Naoki Umezawa, Yoshiya Ikawa
Group I intron ribozymes share common core elements that form a three-dimensional structure responsible for their catalytic activity. This core structure is unstable without assistance from additional factors that stabilize its tertiary structure. We examined biogenic triamine and tetraamine and also their fragments for their abilities to stabilize a structurally unstable group I ribozyme, ΔP5 ribozyme, derived from the Tetrahymena group I intron ribozyme by deleting its large activator module. Biogenic triamine (spermidine) and tetraamine (spermine) efficiently activated the ΔP5 ribozyme under conditions where the ribozyme was virtually inactive...
January 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29337364/the-function-of-emulsions-on-the-biogenic-amine-formation-and-their-indices-of-sea-bass-fillets-dicentrarchus-labrax-stored-in-vacuum-packaging
#2
Yesim Ozogul, Mustafa Durmus, Esmeray Kuley Boga, Yılmaz Uçar, Fatih Ozogul
The impacts of emulsions based on commercial oils on the biogenic amine formation and their indices of vacuumed packed sea bass fillets were investigated. The results showed that among biogenic amines, cadaverine, putrescine, spermidine, spermine, serotonin, dopamine, and agmatine were predominant amines in sea bass fillets stored under vacuum packaging. Significant differences (P < 0.05) in biogenic amines concentrations of vacuumed packed sea bass treated with emulsions were observed. All groups contained histamine lower than 5...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29336947/characterization-of-goose-spms-molecular-characterization-and-expression-profiling-of-spms-in-the-goose-ovary
#3
Bo Kang, Qilin Xu, Ziyu Chen, Yongsheng Wu, Su Yang, Xicheng Yang, Zhao Zhang, Dongmei Jiang
Spermine synthase (SPMS), which converts spermidine into spermine, is essential for normal cell growth and development processes in humans and other mammals, but the molecular characterization and expression profiling of the SPMS gene remain undetermined in goose tissues and ovarian follicles. In this study, the SPMS cDNA sequence of the Sichuan white goose was cloned and analysed, and SPMS mRNA expression was profiled in various tissues and ovarian follicles. The results showed that the open reading frame of the SPMS cDNA sequence was 1092 bp in length, encoding 363 amino acids with a molecular weight of 41 kDa...
January 11, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29301981/controlling-of-n-alkylpolyamine-analogue-metabolism-by-selective-deuteration
#4
Sebahat Ucal, Merja R Häkkinen, Aino-Liisa Alanne, Leena Alhonen, Jouko Vepsäläinen, Tuomo A Keinänen, Mervi T Hyvönen
Replacing protium with deuterium is an efficient method to modulate drug metabolism. N -alkylated polyamine analogues are polyamine antimetabolites with proven anticancer efficacy. We have characterized earlier the preferred metabolic routes of N1,N12 -diethylspermine (DESpm), N1 -benzyl- N12 -ethylspermine (BnEtSpm) and N1,N12 -dibenzylspermine (DBSpm) by human recombinant spermine oxidase (SMOX) and acetylpolyamine oxidase (APAO). Here we studied the above analogues, their variably deuterated counterparts and their metabolites as substrates and inhibitors of APAO, SMOX, semicarbazide-sensitive amine oxidase (SSAO), diamine oxidase (DAO) and monoamine oxidases...
January 4, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29297849/tessaracoccus-terricola-sp-nov-isolated-from-oil-contaminated-soil
#5
Dhiraj Kumar Chaudhary, Jaisoo Kim
A Gram-stain-positive, non-motile, yellow and rod-shaped actinobacterium, designated strain Brt-AT, isolated from oil-contaminated soil, grew at 15-40 °C, at pH 5.5-10.0 and at 0-2 % (w/v) NaCl concentration. This strain was characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain Brt-AT belonged to the genus Tessaracoccus and is closely related to Tessaracoccus rhinocerotis YIM 101269T, Tessaracoccus flavescens SST-39T, Tessaracoccus defluvii LNB-140T and Tessaracoccus flavus RP1T (99...
January 3, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29281982/polyamines-in-the-life-of-arabidopsis-profiling-the-expression-of-s-adenosylmethionine-decarboxylase-samdc-gene-family-during-its-life-cycle
#6
Rajtilak Majumdar, Lin Shao, Swathi A Turlapati, Subhash C Minocha
BACKGROUND: Arabidopsis has 5 paralogs of the S-adenosylmethionine decarboxylase (SAMDC) gene. Neither their specific role in development nor the role of positive/purifying selection in genetic divergence of this gene family is known. While some data are available on organ-specific expression of AtSAMDC1, AtSAMDC2, AtSAMDC3 and AtSAMDC4, not much is known about their promoters including AtSAMDC5, which is believed to be non-functional. RESULTS: (1) Phylogenetic analysis of the five AtSAMDC genes shows similar divergence pattern for promoters and coding sequences (CDSs), whereas, genetic divergence of 5'UTRs and 3'UTRs was independent of the promoters and CDSs; (2) while AtSAMDC1 and AtSAMDC4 promoters exhibit high activity (constitutive in the former), promoter activities of AtSAMDC2, AtSAMDC3 and AtSAMDC5 are moderate to low in seedlings (depending upon translational or transcriptional fusions), and are localized mainly in the vascular tissues and reproductive organs in mature plants; (3) based on promoter activity, it appears that AtSAMDC5 is both transcriptionally and translationally active, but based on it's coding sequence it seems to produce a non-functional protein; (4) though 5'-UTR based regulation of AtSAMDC expression through upstream open reading frames (uORFs) in the 5'UTR is well known, no such uORFs are present in AtSAMDC4 and AtSAMDC5; (5) the promoter regions of all five AtSAMDC genes contain common stress-responsive elements and hormone-responsive elements; (6) at the organ level, the activity of AtSAMDC enzyme does not correlate with the expression of specific AtSAMDC genes or with the contents of spermidine and spermine...
December 28, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29279818/differential-levels-of-metabolites-and-enzymes-related-to-aroma-formation-in-aromatic-indica-rice-varieties-comparison-with-non-aromatic-varieties
#7
Puja Ghosh, Aryadeep Roychoudhury
Accounting for aroma production in different aromatic indica rice varieties based on variations in the levels of concerned metabolites and enzymes is poorly explored. The present investigation was, therefore, focused on unraveling the differential levels of metabolites and activities of enzymes related to aroma formation in eleven indigenous aromatic rice varieties, as compared with four non-aromatic varieties. The levels of metabolites such as proline (Pro) and Δ1-pyrroline-5-carboxylate (P5C), and the activity of related enzymes such as proline dehydrogenase (PDH), Δ1-pyrroline-5-carboxylate synthetase (P5CS), and ornithine aminotransferase (OAT) were comparatively higher in the aromatic varieties, with Kalonunia and Tulaipanji registering the highest Pro, Kalonunia the highest P5C content, Gobindobhog with the highest PDH activity, Gobindobhog and Tulaipanji with the highest P5CS, and Pusa Basmati-1 with the highest OAT activity...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29249019/acrolein-toxicity-at-advanced-age-present-and-future
#8
REVIEW
Kazuei Igarashi, Takeshi Uemura, Keiko Kashiwagi
It is thought that tissue damage at advanced age is mainly caused by ROS (reactive oxygen species, O2-, H2O2, and ·OH). However, it was found that acrolein (CH2=CH-CHO) is more toxic than ROS, and is mainly produced from spermine (SPM), one of the polyamines, rather than from unsaturated fatty acids. Significant amounts of SPM are present normally as SPM-ribosome complexes, and contribute to protein synthesis. However, SPM was released from ribosomes due to the degradation of ribosomal RNA by ·OH or the binding of Ca2+ to ribosomes, and acrolein was produced from free SPM by polyamine oxidases, particularly by SPM oxidase...
December 16, 2017: Amino Acids
https://www.readbyqxmd.com/read/29248687/nitric-oxide-promotes-epidermal-stem-cell-proliferation-via-foxg1-c-myc-signalling
#9
Rixing Zhan, Fan Wang, Ying Wu, Ying Wang, Wei Qian, Menglong Liu, Tengfei Liu, Weifeng He, Hui Ren, Gaoxing Luo
OBJECTIVE: Epidermal stem cells (ESCs) play a critical role in wound repair, but the mechanism underlying ESC proliferation is unclear. Here, we explored the effects of nitric oxide (NO) on ESC proliferation and the possible underlying mechanism. METHODS: The effect of NO (two NO donors, SNAP and spermine NONOate, were used) on cell proliferation was detected using cell proliferation and DNA synthesis assays. Thereafter, expression of FOXG1 and c-Myc induced by NO was determined by immunoblot analysis...
December 14, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29247733/specific-and-highly-efficient-condensation-of-gc-and-ic-dna-by-polyaza-pyridinophane-derivatives
#10
Marijana Radić Stojković, Jorge Gonzalez-Garcia, Filip Šupljika, Cristina Galiana-Rosello, Lluis Guijarro, Salvatore A Gazze, Lewis W Francis, Ivo Piantanida, Enrique Garcia-Espana
Two bis-polyaza pyridinophane derivatives and their monomeric reference compounds revealed strong interactions with ds-DNA and RNA. The bis-derivatives show a specific condensation of GC- and IC-DNA, which is almost two orders of magnitude more efficient than the well-known condensation agent spermine. The type of condensed DNA was identified as ψ-DNA, characterized by the exceptionally strong CD signals. At variance to the almost silent AT(U) polynucleotides, these strong CD signals allow the determination of GC-condensates at nanomolar nucleobase concentrations...
December 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29247730/collapse-of-dna-in-packaging-and-cellular-transport
#11
REVIEW
T J Thomas, Thresia Thomas
The dawn of molecular biology and recombinant DNA technology arose from our ability to manipulate DNA, including the process of collapse of long extended DNA molecules into nanoparticles of approximately 100 nm diameter. This condensation process is important for the packaging of DNA in the cell and for transporting DNA through the cell membrane for gene therapy. Multivalent cations, such as natural polyamines (spermidine and spermine), were initially recognized for their ability to provoke DNA condensation...
December 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29239473/polyamines-regulating-phytic-acid-degradation-in-mung-bean-sprouts
#12
Ting Zhou, Pei Wang, Runqiang Yang, Zhenxin Gu
BACKGROUND: Polyamines are essentially involved in cell division and differentiation. Transport of polyamine is ATP dependent, while phytic acid is the major reserve of phosphate essential to the energy-producing machinery of cells. Thus, polyamines might enhance phytic acid degradation during mung bean germination. In this study, different polyamines [putrescine (Put), spermidine (Spd) and spermine (Spm)] and dicyclohexylamine (DCHA, a Spd synthesis inhibitor) were applied to investigate the function of polyamines on phytic acid degradation...
December 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29232652/effect-of-ornithine-decarboxylase-and-norspermidine-in-modulating-cell-division-in-the-green-alga-chlamydomonas-reinhardtii
#13
Annalisa Tassoni, Nahid Awad, Gareth Griffiths
The extensive genetic resources of Chlamydomonas has led to its widespread use as a model system for understanding fundamental processes in plant cells, including rates of cell division potentially modulated through polyamines. Putrescine was the major polyamine in both free (88%) and membrane-bound fractions (93%) while norspermidine was the next most abundant in these fractions accounting for 11% and 6%, respectively. Low levels of diaminopropane, spermidine and spermine were also observed although no cadaverine or norspermine were detected...
December 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29222130/stargazin-and-cornichon-3-relieve-polyamine-block-of-ampa-receptors-by-enhancing-blocker-permeation
#14
Patricia M G E Brown, Hugo McGuire, Derek Bowie
Most ligand- and voltage-gated ion channels assemble as signaling complexes consisting of pore-forming and auxiliary subunits. In the mammalian brain, AMPA-type ionotropic glutamate receptors (AMPARs) coassemble with several families of auxiliary subunits that regulate channel gating as well as ion channel block and permeation. Previous work has shown that auxiliary proteins stargazin (or γ2) and cornichon-3 (CNIH-3) attenuate the cytoplasmic polyamine channel block of AMPARs, although the underlying mechanism has yet to be established...
December 8, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/29216408/a-cold-responsive-erf-from-medicago-falcata-confers-cold-tolerance-by-up-regulation-of-polyamine-turnover-antioxidant-protection-and-proline-accumulation
#15
Chunliu Zhuo, Lu Liang, Yaqing Zhao, Zhenfei Guo, Shaoyun Lu
ERF subfamily transcription factors play an important role in plant abiotic and biotic stress tolerance. A cold responsive ethylene responsive factor (ERF), MfERF1, was isolated from Medicago falcata, an important forage legume that has great cold tolerance. Overexpression of MfERF1 resulted in an increased tolerance to freezing and chilling in transgenic tobacco plants, while down-regulation of the ortholog of MfERF1 in M. truncatula resulted in reduced freezing tolerance in RNAi plants. Higher transcript levels of some stress responsive genes (CHN50, OSM, ERD10C, and SAMS) and those involved in spermidine (Spd) and spermine (Spm) synthesis (SAMDC1, SAMDC2, SPDS1, SPDS2, and SPMS) and catabolism (PAO) were observed in transgenic plants than in WT...
December 7, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29215586/investigation-of-polyamine-metabolism-and-homeostasis-in-pancreatic-cancers
#16
Chelsea Massaro, Jenna Thomas, Otto Phanstiel Iv
Pancreatic cancers are currently the fourth leading cause of cancer-related death and new therapies are desperately needed. The most common pancreatic cancer is pancreatic ductal adenocarcinoma (PDAC). This report describes the development of therapies, which effectively deplete PDAC cells of their required polyamine growth factors. Of all human tissues, the pancreas has the highest level of the native polyamine spermidine. To sustain their high growth rates, PDACs have altered polyamine metabolism, which is reflected in their high intracellular polyamine levels and their upregulated import of exogenous polyamines...
December 7, 2017: Medical Sciences: Open Access Journal
https://www.readbyqxmd.com/read/29201044/ischaemic-preconditioning-suppresses-necrosis-of-adipocutaneous-flaps-in-a-diabetic-rat-model-regardless-of-the-manner-of-preischaemia-induction
#17
Christian Ottomann, Markus Küntscher, Bernd Hartmann, Vlado Antonic
Ischaemic insult in the skin flaps is a major problem in reconstructive surgery particularly in patients with diabetes mellitus. Here, we sought to investigate the effectiveness of ischaemic preconditioning (IP) on diabetic skin flaps in rat animal model. Hundred Wistar rats (90 streptozotocin treated animals and 10 nondiabetic controls) were used. Diabetes mellitus was confirmed by measuring glucose level in blood, HbA1c, and ketonuria. We used blood vessel clamping, hind limb tourniquet, and NO donors (Spermine/NO complex) to induce short-term ischaemia of tissues that will be excised for skin flaps...
2017: Dermatology Research and Practice
https://www.readbyqxmd.com/read/29196820/functional-roles-of-agmatinase-during-the-peri-implantation-period-of-pregnancy-in-sheep
#18
Yasser Y Lenis, Mohammed A Elmetwally, Wanjin Tang, Carey Satterfield, Kathrin Dunlap, Guoyao Wu, Fuller W Bazer
This study investigated the effect of agmatine (Agm) in proliferation of ovine trophecdoderm cells (oTr1) as well as the importance of the arginine decarboxylase (ADC) and agmatinase (AGMAT) alternative pathway for synthesis of polyamines in ovine conceptuses during the peri-implantation period of pregnancy. Morpholino antisense oligonucleotides (MAOs) were used to inhibit translation of mRNAs for ODC1 alone, AGMAT alone, and their combination. Rambouillet ewes (N = 50) were assigned randomly to the following treatments on Day 8 of pregnancy: MAO control (n = 10); MAO-ODC1 (n = 8); MAO-ADC (n = 6); MAO-ODC1:MAO-ADC (n = 9); or MAO-ODC1:MAO-AGMAT (n = 9)...
December 2, 2017: Amino Acids
https://www.readbyqxmd.com/read/29187844/speg-is-required-for-intracellular-replication-of-salmonella-in-various-human-cells-and-affects-its-polyamine-metabolism-and-global-transcriptomes
#19
Shiuh-Bin Fang, Ching-Jou Huang, Chih-Hung Huang, Ke-Chuan Wang, Nai-Wen Chang, Hung-Yin Pan, Hsu-Wei Fang, Ming-Te Huang, Ching-Kuo Chen
The speG gene has been reported to regulate polyamine metabolism in Escherichia coli and Shigella, but its role in Salmonella remains unknown. Our preliminary studies have revealed that speG widely affects the transcriptomes of infected in vitro M and Caco-2 cells and that it is required for the intracellular replication of Salmonella enterica serovar Typhimurium (S. Typhimurium) in HeLa cells. In this study, we demonstrated that speG plays a time-dependent and cell type-independent role in the intracellular replication of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29166726/-interaction-between-trpc1-and-stim1-in-calcium-sensing-receptor-mediated-calcium-influx-and-nitric-oxide-production-in-human-umbilical-vein-endothelial-cells
#20
L M Wang, H Zhong, N Tang, L J Pang, C J Zhang, F He
Objective: To investigate the interaction of Ca(2+) protein TRPC1 and STIM1 in extracellular Ca(2+) -sensing receptor (CaR)-induced extracellular Ca(2+) influx and the production of nitric oxide (NO). Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and incubated with CaR agonist spermine (activating store-operates cation channels (SOC) and receptor-operated channels (ROC)), CaR negative allosteric modulator Calhex231 (blocking SOC, activating ROC) and ROC analogue TPA (activating ROC, blocking SOC), protein kinase C (PKC) inhibitor Ro31-8220, PKCs and PKCμ inhibitor Go6967(activate SOC, blocking ROC), respectively...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
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