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https://www.readbyqxmd.com/read/28433188/metabolic-engineering-of-saccharomyces-cerevisiae-for-production-of-spermidine-under-optimal-culture-conditions
#1
Sun-Ki Kim, Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo
Spermidine is a polyamine compound exhibiting important biological activities, such as increasing lifespan, inflammation reduction, and plant growth control. As such, many applications of spermidine as a bio-modulating agent are anticipated. However, sustainable and scalable production of spermidine has not been achieved yet. Therefore, construction of a spermidine production system using Saccharomyces cerevisiae was attempted in this study. In order to secrete spermidine into fermentation broth, TPO1 coding for the polyamine transporter was overexpressed in an engineered S...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28433171/gellan-gum-based-physical-hydrogels-incorporating-highly-valuable-endogen-molecules-and-associating-bmp-2-as-bone-formation-platforms
#2
Rita López-Cebral, Ana Civantos, Viviana Ramos, Begoña Seijo, José Luis López-Lacomba, José Vicente Sanz-Casado, Alejandro Sanchez
Physical hydrogels have been designed for a double purpose: as growth factor delivery systems and as scaffolds to support cell colonization and formation of new bone. Specifically, the polysaccharide gellan gum and the ubiquitous endogenous molecules chondroitin, albumin and spermidine have been used as exclusive components of these hydrogels. The mild ionotropic gelation technique was used to preserve the bioactivity of the selected growth factor, rhBMP-2. In vitro tests demonstrated the effective delivery of rhBMP-2 in its bioactive form...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28432550/estradiol-mitigates-ischemia-reperfusion-induced-acute-renal-failure-through-nmda-receptor-antagonism-in-rats
#3
Amrit Pal Singh, Nirmal Singh, Preet Mohinder Singh Bedi
In the present study, we investigated possible involvement of N-methyl-D-aspartate receptors (NMDAR) in estradiol mediated protection against ischemia reperfusion (I/R)-induced acute renal failure (ARF) in rats. Bilateral renal ischemia of 40 min followed by reperfusion for 24 h induced ARF in male wistar rats. Quantification of serum creatinine, creatinine clearance (CrCl), blood urea nitrogen (BUN), uric acid, potassium, fractional excretion of sodium (FeNa), and urinary microproteins was done to assess I/R-induced renal damage in rats...
April 21, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28431088/stationary-phase-genes-upregulated-by-polyamines-are-responsible-for-the-formation-of-escherichia-coli-persister-cells-tolerant-to-netilmicin
#4
Alexander G Tkachenko, Natalya M Kashevarova, Elena A Tyuleneva, Mikhail S Shumkov
Persisters are rare phenotypic variants of regular bacterial cells that survive lethal antibiotics or stresses owing to slowing down of their metabolism. Recently, we have shown that polyamine putrescine can upregulate persister cell formation in Escherichia coli via the stimulation of rpoS expression, encoding a master regulator of general stress response. We hypothesized that rmf and yqjD, the stationary-phase genes responsible for ribosome inactivation, might be good candidates for the similar role owing to their involvement in translational arrest and the ability to be affected by polyamines...
April 19, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28429317/biochemistry-and-physiology-of-reactive-oxygen-species-in-euglena
#5
Takahiro Ishikawa, Shun Tamaki, Takanori Maruta, Shigeru Shigeoka
Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide are by-products of various metabolic processes in aerobic organisms including Euglena. Chloroplasts and mitochondria are the main sites of ROS generation by photosynthesis and respiration, respectively, through the active electron transport chain. An efficient antioxidant network is required to maintain intracellular ROS pools at optimal conditions for redox homeostasis. A comparison with the networks of plants and animals revealed that Euglena has acquired some aspects of ROS metabolic process...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28429226/screening-of-some-dioxaboreninopyridine-and-aniline-derivatives-for-carcinogenic-properties-using-a-model-cell-free-system-of-regenerating-rat-liver
#6
S P Syatkin, A A Kirichuk, A T Soldatenkov, S V Kutyakov, E V Neborak, N A Shevkun, O M Kuznetsova, A S Skorik, A A Terent'ev
We studied the effects of some aniline and dioxaborininopyridine derivatives on the rate of oxidative deamination of putrescine and polyamines in a tissue with high mitotic index. These effects were evaluated quantitatively by measuring diamine oxidase and polyamine oxidase activities in a model cell-free test system of regenerating rat liver tissue. Aniline derivatives exhibited mainly antiproliferative effects and promoted oxidative degradation of putrescine, spermidine, and spermine. Dioxaborininopyridine derivatives inhibited this process, thus exhibiting carcinogenic properties...
April 21, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28428760/the-variation-tendency-of-polyamines-forms-and-components-of-polyamine-metabolism-in-zoysiagrass-zoysia-japonica-steud-to-salt-stress-with-exogenous-spermidine-application
#7
Shucheng Li, Linlin Cui, Yujuan Zhang, Yunwen Wang, Peisheng Mao
To understand dynamic changes in polyamines (PAs) forms and components of polyamine metabolism in zoysiagrass (Zoysia japonica Steud.) response to salt stress with exogenous spermidine (Spd) application, two Chinese zoysia cultivars, z081 and z057, were exposed to sodium chloride stress for 2, 4, 6, and 8 days. The z057 cultivar possesses higher salinity tolerance than the z081 cultivar. Salt stress decreased the zoysiagrass fresh weight (FW) and increased free Spd and spermine (Spm) levels and soluble and insoluble putrescine (Put), Spd and Spm levels in both cultivars...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28423064/biochemical-evaluation-of-the-anticancer-potential-of-the-polyamine-based-nanocarrier-nano11047
#8
Tracy Murray-Stewart, Elena Ferrari, Ying Xie, Fei Yu, Laurence J Marton, David Oupicky, Robert A Casero
Synthesizing polycationic polymers directly from existing drugs overcomes the drug-loading limitations often associated with pharmacologically inert nanocarriers. We recently described nanocarriers formed from a first-generation polyamine analogue, bis(ethyl)norspermine (BENSpm), that could simultaneously target polyamine metabolism while delivering therapeutic nucleic acids. In the current study, we describe the synthesis and evaluation of self-immolative nanocarriers derived from the second-generation polyamine analogue PG-11047...
2017: PloS One
https://www.readbyqxmd.com/read/28408448/spermidine-promotes-cardioprotective-autophagy
#9
REVIEW
Dan Tong, Joseph A Hill
No abstract text is available yet for this article.
April 14, 2017: Circulation Research
https://www.readbyqxmd.com/read/28407698/spermidine-coupled-with-exercise-rescues-skeletal-muscle-atrophy-from-d-gal-induced-aging-rats-through-enhanced-autophagy-and-reduced-apoptosis-via-ampk-foxo3a-signal-pathway
#10
Jingjing Fan, Xiaoqi Yang, Jie Li, Ziyang Shu, Jun Dai, Xingran Liu, Biao Li, Shaohui Jia, Xianjuan Kou, Yi Yang, Ning Chen
The quality control of skeletal muscle is a continuous requirement throughout the lifetime, although its functions and quality present as a declining trend during aging process. Dysfunctional or deficient autophagy and excessive apoptosis may contribute to the atrophy of senescent skeletal muscle. Spermidine, as a natural polyamine, can be involved in important cellular functions for lifespan extension and stress resistance in several model organisms through activating autophagy. Similarly, cellular autophagic responses to exercise have also been extensively investigated...
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28404523/structure-synthesis-and-biological-properties-of-the-pentacyclic-guanidinium-alkaloids
#11
REVIEW
Yunlong Shi, Yasamin Moazami, Joshua G Pierce
The pentacyclic guanidinium alkaloids (PGAs) are a family of marine natural products that possess a polycyclic guanidine-containing core and a long alkyl chain tethered spermidine-derived tail that is rarely observed in other natural products. These natural products exhibit potent activities on a wide range of organisms and therefore have attracted the attention of many synthetic chemists; however, the structure-activity relationships and mechanisms of action of PGAs remain largely elusive. Herein we summarize the structure, synthesis, toxicity and mechanisms of action of PGAs and highlight their potential as chemical probes and/or therapeutic leads...
March 12, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28402874/a-molecular-view-of-the-dynamics-of-dsdna-packing-inside-viral-capsids-in-the-presence-of-ions
#12
Andrés Córdoba, Daniel M Hinckley, Joshua Lequieu, Juan J de Pablo
Genome packing in viruses and prokaryotes relies on positively charged ions to reduce electrostatic repulsions, and induce attractions that can facilitate DNA condensation. Here we present molecular dynamics simulations spanning several microseconds of dsDNA packing inside nanometer-sized viral capsids. We use a detailed molecular model of DNA that accounts for molecular structure, basepairing, and explicit counterions. The size and shape of the capsids studied here are based on the 30-nanometer-diameter gene transfer agents of bacterium Rhodobacter capsulatus that transfer random 4...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28386731/comparative-metagenomics-reveals-insights-into-the-deep-sea-adaptation-mechanism-of-the-microorganisms-in-iheya-hydrothermal-fields
#13
COMPARATIVE STUDY
Hai-Liang Wang, Li Sun
In this study, comparative metagenomic analysis was performed to investigate the genetic profiles of the microbial communities inhabiting the sediments surrounding Iheya North and Iheya Ridge hydrothermal fields. Four samples were used, which differed in their distances from hydrothermal vents. The results showed that genes involved in cell surface structure synthesis, polyamine metabolism and homeostasis, osmoadaptation, pH and Na(+) homeostasis, and heavy-metal transport were abundant. Pathways for putrescine and spermidine synthesis and transport were identified in the four metagenomes, which possibly participate in the regulation of cytoplasmic pH...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28386016/spermidine-prolongs-lifespan-and-prevents-liver-fibrosis-and-hepatocellular-carcinoma-by-activating-map1s-mediated-autophagy
#14
Fei Yue, Wenjiao Li, Jing Zou, Xianhan Jiang, Guibin Xu, Hai Huang, Leyuan Liu
Liver fibrosis and hepatocellular carcinoma (HCC) have worldwide impact but continue to lack safe, low cost and effective treatments. In this study, we show how the simple polyamine spermidine can relieve cancer cell defects in autophagy which trigger oxidative stress-induced cell death and promote liver fibrosis and HCC.  We found that the autophagic marker protein LC3 interacted with the microtubule-associated protein MAP1S which positively regulated autophagy flux in cells.  MAP1S stability was regulated in turn by its interaction with the histone deacetylase HDAC4...
April 6, 2017: Cancer Research
https://www.readbyqxmd.com/read/28383560/induction-of-autophagy-by-spermidine-is-neuroprotective-via-inhibition-of-caspase-3-mediated-beclin-1-cleavage
#15
Yi Yang, Sicong Chen, Yuqing Zhang, Xiaoxia Lin, Yiyin Song, Zhaoliang Xue, Haoran Qian, Shanshan Wang, Guihua Wan, Xiaoxiang Zheng, Lihui Zhang
Spermidine, a natural polyamine presented widely in mammalian cells, has been implicated to extend the lifespan of several model organisms by inducing autophagy. However, the effect of spermidine against neuronal damage has not yet been fully determined. In this study, neuronal cell injury was induced by treating PC12 cells and cortical neurons with 1 μM staurosporine (STS). We found that STS-induced cell injury could be efficiently attenuated by pretreatment with 1 mM spermidine. Spermidine inhibited the caspase 3 activation induced by STS...
April 6, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28383077/involvement-of-ethylene-and-polyamines-biosynthesis-and-abdominal-phloem-tissues-characters-of-wheat-caryopsis-during-grain-filling-under-stress-conditions
#16
Weibing Yang, Yanxia Li, Yanping Yin, Zhilie Qin, Mengjing Zheng, Jin Chen, Yongli Luo, Dangwei Pang, Wenwen Jiang, Yong Li, Zhenlin Wang
Severe water deficit (SD) severely limited the photo-assimilate supply during the grain-filling stages. Although the ethylene and polyamines (PAs) have been identified as important signaling molecules involved in stress tolerance, it is yet unclear how 1-Aminocylopropane-1-carboxylic acid (ACC) and PA biosynthesis involving wheat abdominal phloem characters mitigate SD-induced filling inhibition. The results obtained indicated that the SD down-regulated the TaSUT1 expression and decreased the activities of sucrose synthase (SuSase, EC2...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28367269/spermidine-oxidation-mediated-degeneration-of-retinal-pigment-epithelium-in-rats
#17
Koji Ohashi, Masaaki Kageyama, Katsuhiko Shinomiya, Yukie Fujita-Koyama, Shin-Ichiro Hirai, Osamu Katsuta, Masatsugu Nakamura
Retinal pigment epithelium (RPE) degeneration is a crucial event in dry age-related macular degeneration and gyrate atrophy. The polyamine spermidine has been shown to induce RPE cell death in vitro. The present study aimed to establish a novel in vivo model of spermidine-induced RPE degeneration and to determine whether spermidine-induced RPE cell death involves oxidative mechanisms. In this study, spermidine caused ARPE-19 cell death in a concentration-dependent manner. This effect was prevented by removal of serum from the culture medium or treatment with amine oxidase inhibitors, N-acetylcysteine (NAC), or aldehyde dehydrogenase (ALDH)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28365977/spermidine-protects-against-oxidative-stress-in-inflammation-models-using-macrophages-and-zebrafish
#18
Jin-Woo Jeong, Hee-Jae Cha, Min Ho Han, Su Jung Hwang, Dae-Sung Lee, Jong Su Yoo, Il-Whan Choi, Suhkmann Kim, Heui-Soo Kim, Gi-Young Kim, Su Hyun Hong, Cheol Park, Hyo-Jong Lee, Yung Hyun Choi
Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), and cytokines including tumor necrosis factor-α and interleukin-1β in RAW 264...
April 6, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28362829/oaz1-knockdown-enhances-viability-and-inhibits-er-and-lhr-transcriptions-of-granulosa-cells-in-geese
#19
Bo Kang, Dongmei Jiang, Rong Ma, Hui He, Zhixin Yi, Ziyu Chen
An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured...
2017: PloS One
https://www.readbyqxmd.com/read/28345120/fission-yeast-srm1-is-involved-in-stress-response-and-cell-cycle
#20
Aslıhan Örs Gevrekci
Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this study, the aim is to experimentally characterize a predicted spermidine synthase gene srm1, which was identified upon sequence similarity, in fission yeast Schizosaccharomyces pombe. In an attempt to understand the role of this gene in cell cycle and stress response, deletion mutant of srm1 was generated and analyzed in terms of cell cycle regulation and environmental stress response...
March 27, 2017: Current Microbiology
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