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Chunfang Tang, Riqing Zhang, Xinjiang Hu, Jinfeng Song, Bing Li, Danling Ou, Xi Hu, Yunlin Zhao
In this study, exogenous spermidine role on Salix matsudana tolerance to cadmium was evaluated. Spermidine and cadmium presented antagonistic effects on the biomass, copper and zinc concentrations in S. matsudana. cadmium mainly distributed in the cell wall of subcellular fraction; 46.97%-60.43% of cadmium existed in a sodium chloride-extracted form. Cadmium contents in roots, leaves, and twigs ranged from 2002.67 to 3961.00, 111.59 to 229.72, and 102.56 to 221.27 mg/kg, respectively. Spermidine application elevated cadmium concentrations in the roots, cuttings, and cell wall and the ratio of deionized water-extracted cadmium, but decreased cadmium levels in the twigs and leaves and the fractions of cadmium extracted by ethanol and sodium chloride, respectively...
January 16, 2019: International Journal of Phytoremediation
Johanna Kuß, Birgit Stallmeyer, Matthias Goldstein, Susanne Rinné, Christiane Pees, Sven Zumhagen, Guiscard Seebohm, Niels Decher, Lutz Pott, Marie-Cécile Kienitz, Eric Schulze-Bahr
BACKGROUND: Inherited forms of sinus node dysfunction (SND) clinically include bradycardia, sinus arrest, and chronotropic incompetence and may serve as disease models to understand sinus node physiology and impulse generation. Recently, a gain-of-function mutation in the G-protein gene GNB2 led to enhanced activation of the GIRK (G-protein activated inwardly rectifying K+ channel). Thus, human cardiac GIRK channels are important for heart rate regulation and subsequently, genes encoding their subunits Kir3...
January 2019: Circulation. Genomic and precision medicine
Xiaoqun Wang, Yiwen Hou, Zhuanghao Hou, Wei Xiong, Guangming Huang
Imaging of cholesterol and other metabolites simultaneously by ambient mass spectrometry will greatly benefit biological studies, however, it still remains challenging. Herein, by adding acid into the desorption electrospray ionization (DESI) spray solvent, we achieved simultaneous mass spectrometry imaging of cholesterol and other metabolites directly from mouse brain sections. The introduction of acid increased the signal intensity of cholesterol in mouse brain tissues by approximately 21-fold. Additionally, present strategy provides increased signal intensities for other metabolites up to 62-folds, as well as identification of seven more metabolites (23 vs 16 for acid enhanced DESI vs DESI)...
January 15, 2019: Analytical Chemistry
Robin Öz, Sriram Kk, Fredrik Westerlund
Single DNA molecule techniques have revolutionized our understanding of DNA-protein interactions. Traditional techniques for such studies have the major drawback that the DNA molecule studied is attached to a bead or a surface. Stretching of DNA molecules in nanofluidic channels has enabled single-molecule studies of DNA-protein interactions without the need of tethering the molecule to a foreign entity. This in turn allows for studying reactions along the whole extension of the molecule, including the free DNA ends...
January 15, 2019: Nanoscale
Kamil Ekici, Hayrettin Okut, Ozgur Isleyici, Yakup Can Sancak, Rabia Mehtap Tuncay
The objective of this study is to measure the amounts of biogenic amines, microbial counts, values of pH, titratable acidity, dry matter, and salt (%) in herby cheese, a very popular staple in the Turkish diet, and to evaluate the concentration of biogenic amines in terms of public health risks. A high-performance liquid chromatography (HPLC) method was used for the determination of eight biogenic amines in 100 herby cheeses sold in the local markets of Van. The bacterial load of the herby cheeses ranged between 4...
January 11, 2019: Foods (Basel, Switzerland)
Yun Zhao, Tingting Zhang, Yang Ning, Danyu Shen, Nianda Yang, Yingying Li, Shan-Ho Chou, Liang Yang, Guoliang Qian
Spermidine is a common polyamine compound produced in bacteria, but its roles remain poorly understood. The bacterial SpeD encodes an S-adenosylmethionine decarboxylase that participates in spermidine synthesis. Lysobacter enzymogenes is an efficient environmental predator of crop fungal pathogens by secreting an antifungal antibiotic HSAF (heat-stable antifungal factor), while Clp is a master transcription factor essential for the antifungal activity of L. enzymogenes. In this work, we observed that speD was a close genomic neighbor of the clp gene...
January 7, 2019: Applied Microbiology and Biotechnology
Kazumasa Hada, Keiko Hirota, Ai Inanobe, Koichiro Kako, Mai Miyata, Sho Araoi, Masaki Matsumoto, Reiya Ohta, Mitsuhiro Arisawa, Hiroaki Daitoku, Toshikatsu Hanada, Akiyoshi Fukamizu
The tricarboxylic acid (TCA) cycle (or citric acid cycle) is responsible for the complete oxidation of acetyl-CoA and formation of intermediates required for ATP production and other anabolic pathways, such as amino acid synthesis. Here, we uncovered an additional mechanism that may help explain the essential role of the TCA cycle in the early embryogenesis of Caenorhabditis elegans We found that knockdown of citrate synthase ( cts-1 ), the initial and rate-limiting enzyme of the TCA cycle, results in early embryonic arrest, but that this phenotype is not due to ATP and amino acids depletion...
January 3, 2019: Journal of Biological Chemistry
Pranav Vemula, Yu Jing, Hu Zhang, Jerry B Hunt, Leslie A Sandusky-Beltran, Daniel C Lee, Ping Liu
Tauopathies consist of intracellular accumulation of hyperphosphorylated and aggregated microtubule protein tau, which remains a histopathological feature of Alzheimer's disease (AD) and frontotemporal dementia. L-Arginine is a semi-essential amino acid with a number of bioactive molecules. Its downstream metabolites putrescine, spermidine, and spermine (polyamines) are critically involved in microtubule assembly and stabilization. Recent evidence implicates altered arginine metabolism in the pathogenesis of AD...
January 2, 2019: Amino Acids
Philippe de Medina
Despite intensive research, no satisfactory therapeutic options have been found for aging and age-related diseases. The British scientist Leslie Orgel stated that evolution is cleverer than we are. This assumption seems correct considering that some species are naturally able to resist the age-related diseases that remain unsolved by our modern medicine. Indeed, bowhead whales can live for more than two hundred years and are suspected to possess efficient antitumor mechanisms. Naked mole-rats are exceptionally long-lived compared to similar-sized mammals and are protected from senescence and age-related diseases...
January 2019: Medical Hypotheses
Priscila R Guerra, Ana Herrero-Fresno, Victor Ladero, Begoña Redruello, Teresa Pires Dos Santos, Malene R Spiegelhauer, Lotte Jelsbak, John Elmerdahl Olsen
BACKGROUND: Avian pathogenic Escherichia coli (APEC) is the infectious agent of a wide variety of avian diseases, which causes substantial economic losses to the poultry industry worldwide. Polyamines contribute to the optimal synthesis of nucleic acids and proteins in bacteria. The objectives of this study were to investigate; i) whether APEC E. coli encodes the same systems for biosynthesis and uptake as described for E. coli K12 and ii) the role of polyamines during in vitro growth of an avian pathogenic E...
December 27, 2018: BMC Microbiology
Albany Reséndiz-Mora, Carla Landa, Sandra Sánchez-Barbosa, Sergio Meza-Toledo, Juan Carlos Santiago-Hernández, Carlos Wong, Isabel Baeza, Carlos Wong-Baeza
Non-bilayer phospholipid arrangements (NPA) are lipid associations different from the bilayer, formed by the interactions of conic anionic lipids and divalent cations that produce an inverted micelle which is inserted between the lipid layers, so the polar heads of the outer lipids spread and expose new antigens. Since these structures are transient, they are not immunogenic, but if they are stabilized by drugs, such as chlorpromazine, they become immunogenic and induce anti-NPA antibodies that trigger a lupus-like disease in mice...
December 20, 2018: Biochemical and Biophysical Research Communications
Kazuei Igarashi, Keiko Kashiwagi
Polyamines, consisting of putrescine, spermidine and spermine are essential for normal cell growth and viability in eukaryotic cells. Since polyamines are cations, they interact with DNA, ATP, phospholipids, specific kinds of proteins, and especially with RNA. Consequently, the functions of these acidic compounds and some proteins are modified by polyamines. In this review, the functional modifications of these molecules by polyamines are presented. Structural change of specific mRNAs by polyamines causes the stimulation of the synthesis of several different proteins, which are important for cell growth and viability...
December 19, 2018: International Journal of Biochemistry & Cell Biology
Bengt-Olof Nilsson, Lo Persson
Recent and exciting in vivo studies show that supplementation with the polyamine spermidine (Spd) is cardioprotective and prolongs lifespan in both mice and humans. The mechanisms behind Spd-induced cardioprotection are supposed to involve Spd-evoked stimulation of autophagy, mitophagy and mitochondrial respiration and improved the mechano-elastical function of cardiomyocytes. Although cellular uptake of Spd was not characterized, these results suggest that Spd is imported by the cardiomyocytes and acts intracellularly...
December 21, 2018: Biochemical Society Transactions
Johanna M Gostner, Dietmar Fuchs
No abstract text is available yet for this article.
December 21, 2018: American Journal of Clinical Nutrition
Suparaporn Khanthasuwan, Aran Incharoensakdi, Saowarath Jantaro
The responses of Synechocystis sp. PCC 6803 exposed to UVA, UVB and UVC for at least 3 h were investigated with the emphasis on the changes of polyamines (PAs) levels in whole cells, thylakoid membrane fraction, and thylakoid membrane-associated proteins fraction. All UV radiations caused a slight decrease on cell growth but a drastic reduction of photosynthetic efficiency of Synechocystis cells. UV radiations, especially UVB and UVC, severely decreased the levels of PAs associated with thylakoid membrane proteins...
December 19, 2018: World Journal of Microbiology & Biotechnology
Olga N Ivanova, Anastasiya V Snezhkina, George S Krasnov, Vladimir T Valuev-Elliston, Olga A Khomich, Alexey R Khomutov, Tuomo A Keinanen, Leena Alhonen, Birke Bartosch, Anna V Kudryavtseva, Sergey N Kochetkov, Alexander V Ivanov
Tumorigenesis is accompanied by the metabolic adaptation of cells to support enhanced proliferation rates and to optimize tumor persistence and amplification within the local microenvironment. In particular, cancer cells exhibit elevated levels of biogenic polyamines. Inhibitors of polyamine biosynthesis and inducers of their catabolism have been evaluated as antitumor drugs, however, their efficacy and safety remain controversial. Our goal was to investigate if drug-induced modulation of polyamine metabolism plays a role in dedifferentiation using differentiated human hepatocyte-like HepaRG cell cultures...
December 18, 2018: Cells
Jae-Chan Lee, Jun-Soo Song, Kyung-Sook Whang
A Gram-stain negative, aromatic compound degrading bacterium, designated strain ASA28T , was isolated from the rhizosphere soil of a pine tree at Anmyon island, Taean in Korea. Strain ASA28T was found to be strictly aerobic, non-motile, short rods which can grow at 15-28 °C (optimum, 25-28 °C), at pH 5.0-11.0 (optimum, pH 7.0) and at salinities of 0-1.0% (w/v) NaCl (optimum, 0% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain ASA28T belongs to the genus Sphingobium, showing high sequence similarity to Sphingobium scionense WP01T (97...
December 18, 2018: Antonie Van Leeuwenhoek
Yuan Chen, Lingjun Yu, Fengquan Liu, Liangcheng Du
Heat-Stable Antifungal Factor (HSAF) and its analogs are antifungal natural products produced by the biocontrol agent Lysobacter enzymogenes . The production of HSAF is greatly influenced by environmental stimuli and nutrients, but the underlying molecular mechanism is mostly unclear. Here, we found that HSAF production in L. enzymogenes OH11 is strictly controlled by spermidine, which is the most prevalent triamine in bacteria. When added into OH11 cultures, spermidine regulated the production of HSAF and analogs in a concentration-dependent manner...
2018: Frontiers in Microbiology
Linda I Hu, Ekaterina V Filippova, Joseph Dang, Sergii Pshenychnyi, Jiapeng Ruan, Olga Kiryukhina, Wayne F Anderson, Misty L Kuhn, Alan J Wolfe
Spermidine N-acetyltransferase (SpeG) acetylates and thus neutralizes toxic polyamines. Studies indicate that SpeG plays an important role in virulence and pathogenicity of many bacteria, which have evolved SpeG-dependent strategies to control polyamine concentrations and survive in their hosts. In Escherichia coli, the two-component response regulator RcsB is reported to be subject to Nε-acetylation on several lysine residues, resulting in reduced DNA binding affinity and reduced transcription of the small RNA rprA; however, the physiological acetylation mechanism responsible for this behavior has not been fully determined...
2018: PloS One
Tuan Manh Nguyen, Jaisoo Kim
A strictly aerobic, orange-pigmented strain was isolated and designated as UCM-25T . This strain is capable of degrading aniline and benzene, while is also producing antimicrobial compounds which inhibit the growth of some common pathogenic microbes. A near full-length 16S rRNA gene sequence revealed similarity to Sphingobium chlorophenolicum NBRC 16172T (98.6%). The level of DNA-DNA hybridization between the new isolate and the related species suggests UCM-25T to be a new species belonging to the genus Sphingobium...
December 17, 2018: Archives of Microbiology
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