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Jerzy Dziuba, Iwona Szerszunowicz, Dorota Nałęcz, Marta Dziuba
BACKGROUND: Proteomic analysis is emerging as a highly useful tool in food research, including studies of food allergies. Two-dimensional gel electrophoresis involving isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis is the most effective method of separating hundreds or even thousands of proteins. In this study, albumin and globulin tractions of pea seeds cv. Ramrod were subjected to proteomic analysis. Selected potentially alergenic proteins were identified based on their molecular weights and isoelectric points...
April 2014: Acta Scientiarum Polonorum. Technologia Alimentaria
F De La Torre Morín, I Sánchez Machín
In the reaction of immediate hypersensibility to alergene is joined to its specific type IgE antibody, also united to the high affinity receptors for IgE (FccI) of the effecters cells fundamentally mastocites and basophiles. The interbreeding of these molecules Fcc to RI, after the union ofpolyvalent antigenes to IgE, active these cells, producing three biologic responses: excitosis of the preformed content of its granules, synthesization of lipidic mediators and citoquine secretion. The inflammation mediators are in last term, substances responsible of the clinic symptomatology...
2006: Anales Otorrinolaringológicos Ibero-americanos
K Jamrozik
Two diagnostics sets Pharmacia--Phadebas RAST and Immuno CAP System determining the concentration of specific IgE antibodies in blood serum were compared with each other and with skin prick tests (SPT). Two common alergens--Dermatophagoides pteronyssinus and Dermatophagoides farinae were evaluated. Analysis of ROC curves shows that maximum specificity and sensitivity was obtained when the cut off value of RAST and CAP reached to 4.9 PRU/ml and 7.3 kU/l respectively. Our date shows the good correlation between RAST,CAP and SPT...
2001: Pneumonologia i Alergologia Polska
K L May, T Hofman
Sensitivity to cats fur alergen, Fel. d. 1 is presented as the second most important cause, after allergy to mites, of perennial atopic asthma. The authors collected the data from literature concerning the concentrations of Fel. d. 1 in homes and public places. Further the structure and production of Fel d. 1 also its cross reactivity and the methods of it's elimination from the environment are described and discussed. Authors own observations of 20 cases of cats fur asthma and atopic dermatitis support the opinion that only half of the patients suspect cats as the cause of their illness and cats fur sensitivity is always accompanied by inhalant or food allergy...
2000: Przegla̧d Lekarski
S Paranos, S Petrović, I Vojović
INTRODUCTION: Skin tests are the most useful single modality for demonstrating an IgE-mediated mechanism underlying clinical symptoms. Skin reactivity to an allergen depends on person's exposure and genetic factors influencing the IgE response. However, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. These variations have led to controversy regarding clinical relevancy of allergy skin testing [1-7]...
September 1998: Srpski Arhiv za Celokupno Lekarstvo
O Cromwell, M G Harries, J Pepys
A group of forty-two steroid-dependent perennial asthmatics was studied over a 13-week period incuding the 8 weeks of a double-blind controlled trial of oral sodium cromoglycate. Given at a dose of 200 mg four times per day the drug provided no significant benefit to the patients when compared with results for those on placebo. Blood from eleven patients consistently failed to release more than 25% of the total cellular histamine when challenged with a range of concentrations of antibody to IgE. All six of the cryptogenic (intrinsic) asthmatics in the trial fell within this group, together with 2/12 asthmatics with negative skin tests but suggestive clinical histories implicating common alergens, and 3/24 extrinsic asthmastics with positive skin prick tests...
July 1980: Clinical Allergy
H Requejo
There were 1762 fungi isolated from the atmosphere of Trujillo city (Peru) during the year 1971. These isolants belonged to 23 genera of fungi. The most frequently isolated were: Cladosporium, Penicillium, Fusarium, Stemphylium, Alternaria, Helminthosporium, Aspergillus and Tilachlidium. Nine genera are reported for first time from the air of the city (Tilachlidium, Cephalosporium, Mucor, Aureobasidium, Hormiscium, Trichoderma, Stachybotrys, Paecilomyces and Fisidium). Six genera may be alergenic for asthmatic patients of Trujillo city...
July 30, 1975: Mycopathologia
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