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https://www.readbyqxmd.com/read/28538363/the-effect-of-alpha-linolenic-acid-on-glycemic-control-in-individuals-with-type-2-diabetes-a-systematic-review-and-meta-analysis-of-randomized-controlled-clinical-trials
#1
Elena Jovanovski, Dandan Li, Hoang Vi Thanh Ho, Vladimir Djedovic, Any de Castro Ruiz Marques, Esra Shishtar, Sonia Blanco Mejia, John L Sievenpiper, Russell J de Souza, Lea Duvnjak, Vladimir Vuksan
BACKGROUND: Polyunsaturated fats (PUFAs) have been shown to reduce type 2 diabetes (T2DM) risk and improve insulin responsiveness in T2DM subjects, but whether the plant sources of omega-3 PUFA (alpha-linolenic acid [ALA]) have an effect on glycemic control requires further investigation. METHODS: The parameters of interest were glycated hemoglobin (HbA1c), fasting blood glucose (FBG), fasting blood insulin (FBI), homeostatic model assessment for insulin resistance (HOMA-IR), fructosamine, and glycated albumin...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28501217/edta-functionalized-magnetic-nanoparticles-a-suitable-platform-for-the-analysis-of-low-abundance-urinary-proteins
#2
Paulo Bastos, Fábio Trindade, Rita Ferreira, Adelino Leite-Moreira, Inês Falcão-Pires, Bruno Manadas, Ana L Daniel-da-Silva, Rui Vitorino
Urine is a highly attractive source of biological information and disease biomarkers, whose proteome characterization is ongoing. To that end, depletion/enrichment strategies for protein analysis can be of great convenience. We have thus developed a method based on the use of EDTA-functionalized magnetic nanoparticles (NPs@EDTA), to fractionate urine samples before liquid chromatography-mass spectrometry analysis and compared the identified proteins with those obtained from ultrafiltrated/unfractionated (UF) urine samples...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28493600/endurance-training-selectively-increases-hdl-bound-sphingosine-1-phosphate-in-the-plasma
#3
Monika Książek, Małgorzata Charmas, Andrzej Klusiewicz, Piotr Zabielski, Barbara Długołęcka, Adrian Chabowski, Marcin Baranowski
Sphingosine-1-phosphate (S1P) is a bioactive lysosphingolipid that is found in relatively high concentration in human plasma. Erythrocytes, endothelial cells, and activated platelets are the main sources of circulating S1P. The majority of plasma S1P is transported bound to high-density lipoprotein (HDL) and albumin. In recent years HDL-bound S1P attracted much attention due to its cardioprotective and anti-atherogenic properties. We have previously found that endurance-trained athletes are characterized by higher plasma S1P concentration compared to untrained individuals...
May 11, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28493490/the-protective-effect-of-humic-rich-substances-on-atypical-aeromonas-salmonicida-subsp-salmonicida-infection-in-common-carp-cyprinus-carpio-l
#4
G Yamin, R Falk, R R Avtalion, N Shoshana, T Ofek, R Smirnov, G Rubenstein, J van Rijn
When challenged with atypical Aeromonas salmonicida subsp. salmonicida, exposure of the common carp (Cyprinus carpio L.) to different humic-rich compounds resulted in a significant reduction in infection rates. Specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a Leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the control treatment...
May 11, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28476156/nutritional-deficiencies-in-homeless-persons-with-problematic-drinking-a-systematic-review
#5
REVIEW
Sharea Ijaz, Joni Jackson, Helen Thorley, Katie Porter, Clare Fleming, Alison Richards, Adrian Bonner, Jelena Savović
BACKGROUND: A significant proportion of homeless people drink alcohol excessively and this can lead to malnutrition and consequent medical problems. The aim of this review was to assess the evidence on the range of nutritional deficiencies in the homeless problem-drinking populations. METHODS: We conducted a comprehensive search of nine scientific literature databases and 13 grey literature sources. We included studies of any design that included homeless population with problem-drinking and reported measures of nutritional deficiencies in urine or blood...
May 5, 2017: International Journal for Equity in Health
https://www.readbyqxmd.com/read/28474540/generation-of-canine-induced-extraembryonic-endoderm-like-cell-line-that-forms-both-extraembryonic-and-embryonic-endoderm-derivatives
#6
Toshiya Nishimura, Naoya Unezaki, Ryoji Kanegi, Himali Daluthgamage Patsy Wijesekera, Shingo Hatoya, Kikuya Sugiura, Noritoshi Kawate, Hiromichi Tamada, Hiroshi Imai, Toshio Inaba
Extraembryonic endoderm (XEN) cells are stem cell lines derived from primitive endoderm cells of inner cell mass in blastocysts. These cells have self-renewal properties and differentiate into visceral endoderm (VE) and parietal endoderm (PE) of the yolk sac. Recently, it has been reported that XEN cells can contribute to fetal embryonic endoderm and their unique potency has been evaluated. Here, we have described the induction and characterization of new canine stem cell lines that closely resemble to XEN cells...
May 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28467334/quality-control-on-radiochemical-purity-in-technetium-99m-radiopharmaceuticals-labelling-three-years-of-experience-on-2280-procedures
#7
Claudio Maioli, Giovanni Luciniani, Aldo Strinchini, Luca Tagliabue, Angelo Del Sole
OBJECTIVE: the purpose of this study was to offer an example of evaluations of the ISO9001 certified internal quality assurance (QA) system of 99mTc-radiopharmaceutical preparations and quality control in vivo use, using industrial kits and generators in order to identify possible sources of errors in the procedures labeling and quality control procedures. METHODS: The study was performed at a single institution over a period of three years (July 1st, 2011 - July 1st, 2014), and included a total of 2280 radiopharmaceutical preparations prepared by four different technologists...
April 28, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/28453249/complete-mapping-of-complex-disulfide-patterns-with-closely-spaced-cysteines-by-in-source-reduction-and-data-dependent-mass-spectrometry
#8
Christian Necip Cramer, Christian D Kelstrup, Jesper Velgaard Olsen, Kim F Haselmann, Peter Kresten Nielsen
Mapping of disulfide bonds is an essential part of protein characterization to ensure correct cysteine pairings. For this, mass spectrometry (MS) is the most widely used technique due to fast and accurate characterization. However, MS-based disulfide mapping is challenged when multiple disulfide bonds are present in complicated patterns. This includes presence of disulfide bonds in nested patterns and closely-spaced cysteines. Unambiguous mapping of such disulfide bonds typically requires advanced MS approaches...
April 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28439279/metabolic-investigation-of-phelipanche-aegyptiaca-reveals-significant-changes-during-developmental-stages-and-in-its-different-organs
#9
Noam Nativ, Yael Hacham, Joseph Hershenhorn, Evgenia Dor, Rachel Amir
Phelipanche aegyptiaca Pers. is a root holoparasitic plant considered to be among the most destructive agricultural weeds worldwide. In order to gain more knowledge about the metabolic profile of the parasite during its developmental stages, we carried out primary metabolic and lipid profiling using GC-MS analysis. In addition, the levels of amino acids that incorporate into proteins, total protein in the albumin fraction, nitrogen, reduced sugars, and phenols were determined. For the assays, the whole plants from the four developmental stages-tubercle, pre-emergent shoot, post-emergent shoot, and mature flowering plants-were taken...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28432127/sex-differences-in-hospitalizations-with-maintenance-hemodialysis
#10
Scott V Adams, Matthew Rivara, Elani Streja, Alfred K Cheung, Onyebuchi A Arah, Kamyar Kalantar-Zadeh, Rajnish Mehrotra
Hospitalization is a major source of morbidity among patients with ESRD undergoing maintenance hemodialysis and is a significant contributor to health care costs. To identify subgroups at the highest risk of hospitalization, we analyzed by sex, age, and race, adjusting for demographic and clinical characteristics, the hospitalization rates, and 30-day readmissions for 333,756 hospitalizations among 111,653 patients undergoing maintenance hemodialysis in facilities operated by a large dialysis organization in the United States (2007-2011)...
April 21, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28420812/isolation-of-mesenchymal-stromal-stem-cells-from-cryopreserved-umbilical-cord-blood-cells
#11
Sumie Fujii, Yasuo Miura, Masaki Iwasa, Satoshi Yoshioka, Aya Fujishiro, Noriko Sugino, Hitomi Kaneko, Yoko Nakagawa, Hideyo Hirai, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Taira Maekawa
Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10(8)) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells...
April 18, 2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28401122/quality-indicators-for-the-detection-and-management-of-chronic-kidney-disease-in-primary-care-in-canada-derived-from-a-modified-delphi-panel-approach
#12
Karen Tu, Lindsay Bevan, Katie Hunter, Jess Rogers, Jacqueline Young, Gihad Nesrallah
BACKGROUND: The detection and management of chronic kidney disease lies within primary care; however, performance measures applicable in the Canadian context are lacking. We sought to develop a set of primary care quality indicators for chronic kidney disease in the Canadian setting and to assess the current state of the disease's detection and management in primary care. METHODS: We used a modified Delphi panel approach, involving 20 panel members from across Canada (10 family physicians, 7 nephrologists, 1 patient, 1 primary care nurse and 1 pharmacist)...
January 2017: CMAJ Open
https://www.readbyqxmd.com/read/28398552/carbamylated-albumin-is-one-of-the-target-antigens-of-anti-carbamylated-protein-antibodies
#13
Shuichiro Nakabo, Motomu Hashimoto, Shinji Ito, Moritoshi Furu, Hiromu Ito, Takao Fujii, Hajime Yoshifuji, Yoshitaka Imura, Ran Nakashima, Kosaku Murakami, Nobuo Kuramoto, Masao Tanaka, Junko Satoh, Akihito Ishigami, Satoshi Morita, Tsuneyo Mimori, Koichiro Ohmura
Objectives.: Anti-carbamylated protein (anti-CarP) antibodies are detected in RA patients. Fetal calf serum is used as an antigen source in anti-CarP ELISA, and the precise target antigens have not been found. We aimed to identify the target antigens of anti-CarP antibodies. Methods.: Western blotting of anti-CarP antibodies was conducted. Anti-carbamylated human albumin (CarALB) antibody was detected by in-house ELISA for 493 RA patients and 144 healthy controls (HCs)...
April 7, 2017: Rheumatology
https://www.readbyqxmd.com/read/28377425/high-density-lipoproteins-selectively-promote-the-survival-of-human-regulatory-t-cells
#14
Cesar M Rueda, Ana Lucia Rodriguez-Perea, Maria Moreno-Fernandez, Courtney M Jackson, John T Melchior, W Sean Davidson, Claire A Chougnet
High-density lipoproteins (HDL) appear to affect regulatory T cell (Treg) homeostasis, as suggested by the increased Treg counts in HDL-treated mice and by the positive correlation between Treg frequency and HDL-C levels in statin-treated healthy adults. However, the underlying mechanisms remain unclear. Herein, we show that HDL, not LDL, significantly decreased the apoptosis of human Treg in vitro, whereas they did not alter naive or memory CD4+ T-cell survival. Similarly, oleic acid bound to serum albumin increased Treg survival...
April 4, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28375742/how-%C3%AE-helical-motifs-form-functionally-diverse-lipid-binding-compartments
#15
Lucy Malinina, Dinshaw J Patel, Rhoderick E Brown
Lipids are produced site-specifically in cells and then distributed nonrandomly among membranes via vesicular and nonvesicular trafficking mechanisms. The latter involves soluble amphitropic proteins extracting specific lipids from source membranes to function as molecular solubilizers that envelope their insoluble cargo before transporting to destination sites. Lipid-binding and lipid transfer structural motifs range from multi-β-strand barrels, to β-sheet cups and baskets covered by α-helical lids, to multi-α-helical bundles and layers...
March 30, 2017: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/28374923/oral-fish-oil-positively-influences-nutritional-inflammatory-risk-in-patients-with-haematological-malignancies-during-chemotherapy-with-an-impact-on-long-term-survival-a-randomised-clinical-trial
#16
T R Chagas, D S Borges, P F de Oliveira, M C Mocellin, A M Barbosa, C Q Camargo, J Â G Del Moral, A Poli, P C Calder, E B S M Trindade, E A Nunes
BACKGROUND: Studies suggest that the ingestion of fish oil (FO), a source of the omega-3 polyunsaturated fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), can reduce the deleterious side-effects of chemotherapy. The aim of this randomised clinical trial was to evaluate the effect of supplementation with oral FO for 9 weeks on nutritional parameters and inflammatory nutritional risk in patients with haematological malignancies during the beginning of chemotherapy...
April 4, 2017: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
https://www.readbyqxmd.com/read/28351677/toxicological-evaluation-of-ethanolic-extract-from-stevia-rebaudiana-bertoni-leaves-genotoxicity-and-subchronic-oral-toxicity
#17
Qiannan Zhang, Hui Yang, Yongning Li, Haibo Liu, Xudong Jia
Stevia rebaudiana Bertoni leaves have a long history of use as an abundant source of sweetener. The aqueous extract of stevia leaves and the predominant constitutes steviol glycosides have been intensively investigated. However, rare studies provided toxicological evaluation of bioactive components in the polar extract regarding their safety on human health. This study aimed to evaluate the toxicity of ethanolic extract of Stevia rebaudiana Bertoni leaves through a battery of in vitro and in vivo tests. Negative results were unanimously obtained from bacterial reverse mutation assay, mouse bone marrow micronucleus assay and mouse sperm malformation assay...
March 25, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28340705/towards-automation-in-protein-digestion-development-of-a-monolithic-trypsin-immobilized-reactor-for-highly-efficient-on-line-digestion-and-analysis
#18
Marina Naldi, Urh Černigoj, Ales Štrancar, Manuela Bartolini
Reducing experimental variability, limiting contamination and increasing automation are essential goals in the development of reliable analytical platforms for mass spectrometry (MS)-based proteomics. In this work novel trypsin-based monolithic immobilized enzyme reactors (tryp-IMERs), obtained by covalent immobilization on convective interaction media (CIMac™) analytical columns (5mm×5.2mm I.D.), were developed. Notwithstanding the small dimensions, column format allowed the insertion in common high performance liquid chromatography (HPLC) systems, thus avoiding the use of expensive micro- or nano-platforms...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28339710/productive-performance-and-blood-profiles-of-laying-hens-fed-hermetia-illucens-larvae-meal-as-total-replacement-of-soybean-meal-from-24-to-45-weeks-of-age
#19
S Marono, R Loponte, P Lombardi, G Vassalotti, M E Pero, F Russo, L Gasco, G Parisi, G Piccolo, S Nizza, C Di Meo, Y A Attia, F Bovera
The aim of the research was to study the effects of an insect meal from Hermetia illucens larvae (HILM) as complete replacement of soybean meal (SBM) on productive performance and blood profiles of laying hens, from 24 to 45 wk of age. A total of 108 24-week-old Lohmann Brown Classic laying hens was equally divided into 2 groups (54 hens/group, 9 replicates of 6 hens/group). From 24 to 45 wk of age, the groups were fed 2 different isoproteic and isoenergetic diets: the control group (SBM) was fed a corn-soybean meal based diet, while in the HILM group the soybean meal was completely replaced by Hermetia illucens larvae meal...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28331804/infected-ascites-distinguishing-secondary-peritonitis-from-spontaneous-bacterial-peritonitis-in-a-cirrhotic-patient-with-classic-symptoms
#20
Marvin Louis Roy Lu, Akanksha Agarwal, Josh Sloan, Aaron Kosmin
INTRODUCTION: Spontaneous bacterial peritonitis can be differentiated from secondary bacterial peritonitis by the absence of a surgically treatable intra-abdominal source of infection. However, oftentimes this is unapparent and other clinical clues need to be sought after to make the right diagnosis. CASE: A 64-year-old woman was admitted because of three days of worsening diffuse abdominal pain and distention. She was morbidly obese and had a history of non-alcoholic steatohepatitis (NASH) cirrhosis...
2017: IDCases
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