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https://www.readbyqxmd.com/read/28340705/towards-automation-in-protein-digestion-development-of-a-monolithic-trypsin-immobilized-reactor-for-highly-efficient-on-line-digestion-and-analysis
#1
Marina Naldi, Urh Černigoj, Ales Štrancar, Manuela Bartolini
Reducing experimental variability, limiting contamination and increasing automation are essential goals in the development of reliable analytical platforms for mass spectrometry (MS)-based proteomics. In this work novel trypsin-based monolithic immobilized enzyme reactors (tryp-IMERs), obtained by covalent immobilization on convective interaction media (CIMac™) analytical columns (5mm×5.2mm I.D.), were developed. Notwithstanding the small dimensions, column format allowed the insertion in common high performance liquid chromatography (HPLC) systems, thus avoiding the use of expensive micro- or nano-platforms...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28339710/productive-performance-and-blood-profiles-of-laying-hens-fed-hermetia-illucens-larvae-meal-as-total-replacement-of-soybean-meal-from-24-to-45-weeks-of-age
#2
S Marono, R Loponte, P Lombardi, G Vassalotti, M E Pero, F Russo, L Gasco, G Parisi, G Piccolo, S Nizza, C Di Meo, Y A Attia, F Bovera
The aim of the research was to study the effects of an insect meal from Hermetia illucens larvae (HILM) as complete replacement of soybean meal (SBM) on productive performance and blood profiles of laying hens, from 24 to 45 wk of age. A total of 108 24-week-old Lohmann Brown Classic laying hens was equally divided into 2 groups (54 hens/group, 9 replicates of 6 hens/group). From 24 to 45 wk of age, the groups were fed 2 different isoproteic and isoenergetic diets: the control group (SBM) was fed a corn-soybean meal based diet, while in the HILM group the soybean meal was completely replaced by Hermetia illucens larvae meal...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28331804/infected-ascites-distinguishing-secondary-peritonitis-from-spontaneous-bacterial-peritonitis-in-a-cirrhotic-patient-with-classic-symptoms
#3
Marvin Louis Roy Lu, Akanksha Agarwal, Josh Sloan, Aaron Kosmin
INTRODUCTION: Spontaneous bacterial peritonitis can be differentiated from secondary bacterial peritonitis by the absence of a surgically treatable intra-abdominal source of infection. However, oftentimes this is unapparent and other clinical clues need to be sought after to make the right diagnosis. CASE: A 64-year-old woman was admitted because of three days of worsening diffuse abdominal pain and distention. She was morbidly obese and had a history of non-alcoholic steatohepatitis (NASH) cirrhosis...
2017: IDCases
https://www.readbyqxmd.com/read/28326316/protein-and-metalloprotein-distribution-in-different-varieties-of-beans-phaseolus-vulgaris-l-effects-of-cooking
#4
Aline P Oliveira, Geyssa Ferreira Andrade, Bianca S O Mateó, Juliana Naozuka
Beans (Phaseolus vulgaris L.) are among the main sources of protein and minerals. The cooking of the grains is imperative, due to reduction of the effect of some toxic and antinutritional substances, as well as increase of protein digestibility. In this study, the effects of cooking on albumins, globulins, prolamins, and glutelins concentration and determination of Fe associated with proteins for different beans varieties and on phaseolin concentration in common and black beans were evaluated. Different extractant solutions (water, NaCl, ethanol, and NaOH) were used for extracting albumins, globulins, prolamins, and glutelins, respectively...
2017: International journal of food science
https://www.readbyqxmd.com/read/28294548/one-pot-cascade-synthesis-and-in-vitro-evaluation-of-anti-inflammatory-and-anti-diabetic-activities-of-s-methylphenyl-substituted-acridine-1-8-diones
#5
Lavanya Mallu, Dhakshanamurthy Thirumalai, Indira Viswambaran Asharani
Various S-methylphenyl substituted acridine-1,8-dione series (4a-i) were synthesized through a one-pot cascade synthetic approach involving the reaction of 4-(methylthio)benzaldehyde and dimedone with a variety of amines as nitrogen source under reflux in ethanol. All the synthesized derivatives were characterized by using spectroscopic methods. In-vitro evaluations of anti-inflammatory and anti-diabetic efficacies of all the synthesized compounds were investigated. The anti-inflammatory results infer that the compounds 4c and 4d are showing excellent activity with an inhibition percentage of 80...
March 13, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28283980/alleviating-the-environmental-heat-burden-on-laying-hens-by-feeding-on-diets-enriched-with-certain-antioxidants-vitamin-e-and-selenium-individually-or-combined
#6
Mohamed E Abd El-Hack, Khalid Mahrose, Muhammad Arif, Maria Tabassum Chaudhry, Islam M Saadeldin, Muhammad Saeed, Rab Nawaz Soomro, Imtiaz Hussain Raja Abbasi, Zaib Ur Rehman
The present study was designed to alleviate the negative biohazards of high ambient temperature on the productive performance and physiological status of laying hens. A total of 135 Bovans laying hens were distributed into nine groups in a 3 × 3 factorial design experiment. Basal diet was supplemented with vitamin E at levels of 0, 250, and 500 mg /kg diet. Within each dietary vitamin E level, each diet was supplemented with sodium selenite as a source of selenium (Se) to supply 0, 0.25, and 0.50 mg Se/kg diet...
March 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28237915/hepatoprotective-activity-of-butea-monosperma-bark-against-thioacetamide-induced-liver-injury-in-rats
#7
Varinder Kaur, Manish Kumar, Paramjeet Kaur, Sandeep Kaur, Amrit Pal Singh, Satwinderjeet Kaur
For thousands of years, the plant-based natural products have been a source of curative agents for various ailments. Butea monosperma (Fabaceae) has an important place in Indian traditional system of medicine for curing number of disorders. The present study deals with evaluation of hepatoprotective properties of ethyl acetate fraction (Beac) from B. monosperma bark in rat model. In preliminary antioxidant studies, Beac demonstrated pronounced superoxide scavenging (IC50 88.85μg/ml) and anti-lipid peroxidation (IC50 131...
February 23, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28231148/potential-use-of-gamma-irradiated-ethnic-ready-to-eat-foods-to-improve-the-nutritional-status-of-landslide-victims
#8
REVIEW
Zubaidah Irawati Koenari, Carmen M Siagian, Bona Simanungkalit, Asti Nilatany, Indra Mustika Pratama, Deudeu Lasmawati, Cecep M Nurcahya
The safety and high quality of ethnic ready-to-eat foods as a source of nourishment and food supplies chain to the remote areas become particularly important. Consuming healthy and balanced nutritional foods means eating better quality foods in proper quantities. Such foods can be developed through a preservation technique by using ionizing radiation. Although implementation of the technology for certain foodstuffs has been implemented in some countries, application of the process to a complete set of meals for selected target groups is still very limited...
July 26, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28223234/advanced-glycation-end-products-produced-systemically-and-by-macrophages-a-common-contributor-to-inflammation-and-degenerative-diseases
#9
REVIEW
Kyunghee Byun, YongCheol Yoo, Myeongjoo Son, Jaesuk Lee, Goo-Bo Jeong, Young Mok Park, Ghasem Hosseini Salekdeh, Bonghee Lee
Advanced glycation end products (AGEs) and their receptor have been implicated in the progressions of many intractable diseases, such as diabetes and atherosclerosis, and are also critical for pathologic changes in chronic degenerative diseases, such as Alzheimer's disease, Parkinson's disease, and alcoholic brain damage. Recently activated macrophages were found to be a source of AGEs, and the most abundant form of AGEs, AGE-albumin excreted by macrophages has been implicated in these diseases and to act through common pathways...
February 13, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28213246/spinel-type-manganese-ferrite-mnfe2o4-microspheres-a-novel-affinity-probe-for-selective-and-fast-enrichment-of-phosphopeptides
#10
Xing-Yu Long, Jia-Yuan Li, Dong Sheng, Hong-Zhen Lian
The spinel-type magnetic manganese ferrite (MnFe2O4) microspheres synthesized by simple solvothermal method were used as a novel adsorbent for selective enrichment and effective isolation of phosphopeptides. The uniform MnFe2O4 magnetic affinity microspheres (MAMSs) had a narrow particle size distribution between 250 and 260nm, and displayed superparamagnetism with a saturation magnetization value of 67.0emu/g. Comprehensively, the possible formation mechanism of MnFe2O4 microspheres with ferric and manganous sources as dual precursors was elucidated by comparison with those of Fe3O4 nanoparticles and MnOOH nanosheets respectively with either ferric or manganous source as single precursor...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28194981/droplet-assisted-inlet-ionization-for-online-analysis-of-airborne-nanoparticles
#11
Andrew J Horan, Michael J Apsokardu, Murray V Johnston
Airborne nanoparticles play a key role in climate effects as well as impacting human health. Their small mass and complex chemical composition represent significant challenges for analysis. This work introduces a new ionization method, droplet assisted inlet ionization (DAII), where aqueous droplets are produced from airborne nanoparticles. When these droplets enter the mass spectrometer through a heated inlet, rapid vaporization leads to the formation of molecular ions. The method is demonstrated with test aerosols consisting of polypropylene glycol (PPG), angiotensin II, bovine serum albumin, and the "thermometer" compound p-methoxybenzylpyridinium chloride...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28164134/diabetes-induced-reactive-oxygen-species-mechanism-of-their-generation-and-role-in-renal-injury
#12
REVIEW
Selim Fakhruddin, Wael Alanazi, Keith E Jackson
Diabetes induces the onset and progression of renal injury through causing hemodynamic dysregulation along with abnormal morphological and functional nephron changes. The most important event that precedes renal injury is an increase in permeability of plasma proteins such as albumin through a damaged glomerular filtration barrier resulting in excessive urinary albumin excretion (UAE). Moreover, once enhanced UAE begins, it may advance renal injury from progression of abnormal renal hemodynamics, increased glomerular basement membrane (GBM) thickness, mesangial expansion, extracellular matrix accumulation, and glomerulosclerosis to eventual end-stage renal damage...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28140559/quantitative-conjugated-payload-measurement-using-enzymatic-release-of-antibody-drug-conjugate-with-cleavable-linker
#13
Brian Rago, L Nathan Tumey, Cong Wei, Frank Barletta, Tracey Clark, Steven Hansel, Xiaogang Han
As antibody-drug conjugate (ADC) design is evolving with novel payload, linker, and conjugation chemistry, the need for sensitive and precise quantitative measurement of conjugated payload to support pharmacokinetics (PK) is in high demand. Compared to ADCs containing noncleavable linkers, a strategy specific to linkers which are liable to pH, chemical reduction, or enzymatic cleavage has gained popularity in recent years. One bioanalytical approach to take advantage of this type of linker design is the development of a PK assay measuring released conjugated payload...
January 31, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28135411/use-of-cryopoor-plasma-for-albumin-replacement-and-continuous-antimicrobial-infusion-for-treatment-of-septic-peritonitis-in-a-dog
#14
Meaghan K Ropski, Julien Guillaumin, Andrea A Monnig, Katy Townsend, Mary A McLoughlin
OBJECTIVE: To report the successful management of a dog with septic peritonitis and septic shock secondary to enterectomy dehiscence using novel techniques for identification of intestinal dehiscence and for septic shock treatment. CASE SUMMARY: A 5-year-old castrated male Bernese Mountain Dog presented for lethargy 6 days following enterotomy for foreign body obstruction. Septic peritonitis was identified due to dehiscence of the enterotomy site, and resection and anastomosis were performed using a gastrointestinal anastomosis and thoracoabdominal stapling device...
January 30, 2017: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/28131026/preparation-and-characterization-of-ph-responsive-polyacrylamide-molecularly-imprinted-polymer-application-to-isolation-of-recombinant-and-wild-type-human-serum-albumin-from-biological-sources
#15
May A Abd El-Aal, Medhat A Al-Ghobashy, Faten Abdel Aziz Fathalla, Yasser S El-Saharty
In this work pH-responsive neutral and cationic polyacrylamide molecularly imprinted polymers (nMIP and cMIP, respectively) were prepared for separation of recombinant and wild type human serum albumin (HSA, pI 4.7) using mixture of polymerization initiators. The effect of pH during preparation and adsorption stages at pI(HSA)±2.0 on binding capacity and selectivity; imprinting factor (IF) was thoroughly investigated. SE-HPLC and RP-HPLC were employed for thorough evaluation of the stability of HSA at the studied experimental conditions and for simultaneous determination of HSA and erythropoietin (EPO) in their mixtures, respectively...
January 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28130827/advanced-glycation-end-products-stimulate-gene-expression-of-fibroblast-growth-factor-23
#16
Ludmilla Bär, Kristin Wächter, Nicole Wege, Alexander Navarrete Santos, Andreas Simm, Michael Föller
SCOPE: Osteoblasts produce fibroblast growth factor 23 (FGF23), a hormone inhibiting renal phosphate reabsorption and the formation of biologically active vitamin D, calcitriol. FGF23-deficient mice age rapidly and develop age-associated diseases at least in part due to massive calcification. Elevated FGF23 serum levels are detected in patients suffering from acute and chronic renal, cardiovascular, inflammatory, and metabolic diseases. Advanced glycation end products (AGEs) are sugar-modified proteins, nucleic acid, and lipids which contribute to these disorders...
January 28, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28116800/the-albumin-bilirubin-grade-improves-hepatic-reserve-estimation-post-sorafenib-failure-implications-for-drug-development
#17
D J Pinato, C Yen, D Bettinger, R Ramaswami, T Arizumi, C Ward, M Pirisi, M E Burlone, R Thimme, M Kudo, R Sharma
BACKGROUND: Drug development in hepatocellular carcinoma (HCC) is limited by disease heterogeneity, with hepatic reserve being a major source of variation in survival outcomes. The albumin-bilirubin (ALBI) grade is a validated index of liver function in patients with HCC. AIM: To test the accuracy of the ALBI grade in predicting post-sorafenib overall survival (PSOS) in patients who permanently discontinued treatment. METHODS: From a prospectively maintained international database of 447 consecutive referrals, we derived 386 eligible patients treated with sorafenib within Barcelona Clinic Liver Cancer C stage (62%), 75% of whom were of Child class A at initiation...
January 24, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28114138/plasma-gelsolin-and-its-association-with-mortality-and-hospitalization-in-chronic-hemodialysis-patients
#18
Cesar Flores Gama, Laura M Rosales, Georges Ouellet, Yanna Dou, Stephan Thijssen, Len Usvyat, Hanjie Zhang, Viktoriya Kuntsevich, Nathan W Levin, Peter Kotanko
BACKGROUND: Human plasma gelsolin (pGSN) is an actin-binding protein that is secreted into the extracellular fluid, with the skeletal muscle and myocardial tissues being its major source. Depletion of pGSN has been shown to be related to a variety of inflammatory and clinical conditions. METHODS: pGSN levels were prospectively determined in prevalent maintenance hemodialysis (HD) patients from 3 U.S. dialysis centers. Demographics (age, time since dialysis initiation, race, gender, body height and weight, comorbidities), inflammatory markers (C reactive protein, CRP; interleukin 6, IL-6), free triiodothyronine (fT3), and routine laboratory parameters were obtained...
2017: Blood Purification
https://www.readbyqxmd.com/read/28110141/synchrotron-fourier-transform-infrared-mapping-a-novel-approach-for-membrane-fouling-characterization
#19
Ming Xie, Wenhai Luo, Stephen R Gray
We described a synchrotron-based infrared (IR) microscopic method to characterize fouling layer induced by organic foulants and colloidal silica in membrane distillation (MD). This technique, utilizing the ultrahigh brightness of synchrotron infrared source, enables spectra with high signal-to-noise ratio that was obtained from micrometer-sized samples. Our results showed that synchrotron IR mapping was able to resolve the foulant spatial distribution in combined fouling in MD. Synchrotron IR mapping showed the spatial distribution of binary foulant (i...
January 11, 2017: Water Research
https://www.readbyqxmd.com/read/28092149/issues-in-nutrition-nutritional-assessment-of-adults
#20
Margaret E Thompson, Mary Barth Noel
The assessment of nutritional status in adults should begin with a complete history, including intake of fruits and vegetables, sources of fat, and added sugar (eg, sugar-sweetened beverages). The history should include social factors that may impede a patient's ability to obtain food, as well as any factors that might interfere with preparing, chewing, and digesting food and absorbing nutrients. The physical examination should include measurement of height and weight and calculation of body mass index. It also may include measurements of waist circumference and waist to hip ratio, and an evaluation of strength...
January 2017: FP Essentials
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