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https://www.readbyqxmd.com/read/29351588/sorghum-root-system-classification-in-contrasting-p-environments-reveals-three-main-rooting-types-and-root-architecture-related-marker-trait-associations
#1
Sebastian Parra-Londono, Mareike Kavka, Birgit Samans, Rod Snowdon, Silke Wieckhorst, Ralf Uptmoor
Background and Aims: Roots facilitate acquisition of macro- and micronutrients, which are crucial for plant productivity and anchorage in the soil. Phosphorus (P) is rapidly immobilized in the soil and hardly available for plants. Adaptation to P scarcity relies on changes in root morphology towards rooting systems well suited for topsoil foraging. Root-system architecture (RSA) defines the spatial organization of the network comprising primary, lateral and stem-derived roots and is important for adaptation to stress conditions...
January 17, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29350486/inflammatory-response-and-treatment-tolerance-of-long-term-infusion-of-the-anti-gd2-antibody-ch14-18-cho-in-combination-with-interleukin-2-in-patients-with-high-risk-neuroblastoma
#2
Kiraz Ceylan, Luciana J Jahns, Bjoern N Lode, Karoline Ehlert, Silke Kietz, Sascha Troschke-Meurer, Nikolai Siebert, Holger N Lode
BACKGROUND: The monoclonal anti-GD2 antibody ch14.18/CHO in combination with IL-2 is active and effective in high-risk neuroblastoma (NB) patients. Here, we investigated the inflammatory response and treatment tolerance of long-term infusion (LTI) of ch14.18/CHO (10 × 10 mg/m2 ; 24 hr) in combination with subcutaneous (s.c.) IL-2 in a single center program. METHODS: Fifty-three NB patients received up to six cycles of 100 mg/m2 ch14.18/CHO (d8-18, where d represents day(s)) as LTI combined with 6 × 106 IU/m2 s...
January 19, 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29347909/identification-of-minor-effect-qtls-for-plant-architecture-related-traits-using-super-high-density-genotyping-and-large-recombinant-inbred-population-in-maize-zea-mays
#3
Baobao Wang, Han Liu, Zhipeng Liu, Xiaomei Dong, Jinjie Guo, Wei Li, Jing Chen, Chi Gao, Yanbin Zhu, Xinmei Zheng, Zongliang Chen, Jian Chen, Weibin Song, Andrew Hauck, Jinsheng Lai
BACKGROUND: Plant Architecture Related Traits (PATs) are of great importance for maize breeding, and mainly controlled by minor effect quantitative trait loci (QTLs). However, cloning or even fine-mapping of minor effect QTLs is very difficult in maize. Theoretically, large population and high density genetic map can be helpful for increasing QTL mapping resolution and accuracy, but such a possibility have not been actually tested. RESULTS: Here, we employed a genotyping-by-sequencing (GBS) strategy to construct a linkage map with 16,769 marker bins for 1021 recombinant inbred lines (RILs)...
January 18, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29347475/micromechanical-model-for-protein-materials-from-macromolecules-to-macroscopic-fibers
#4
G Puglisi, D De Tommasi, M F Pantano, N M Pugno, G Saccomandi
We propose a model for the mechanical behavior of protein materials. Based on a limited number of experimental macromolecular parameters (persistence and contour length) we obtain the macroscopic behavior of keratin fibers (human, cow, and rabbit hair), taking into account the damage and residual stretches effects that are fundamental in many functions of life. We also show the capability of our approach to describe the main dissipation and permanent strain effects observed in the more complex spider silk fibers...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29344161/uvb-radiation-represses-cyld-expression-in-melanocytes
#5
Silke Kuphal, Nadja Schneider, Ramin Massoumi, Claus Hellerbrand, Anja Katrin Bosserhoff
CYLD lysine 63 deubiquitinase (CYLD) was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Unlike in cylindromatosis, downregulation of the deubiquitinase CYLD in melanoma, a highly aggressive tumor, is not caused by mutations in the CYLD gene, but rather by a constitutive and high expression of the snail family transcriptional repressor 1 (SNAIL1). A reduced CYLD level leads to B-cell lymphoma-3/p50/p52-dependent nuclear factor-κB activation, which in turn triggers the expression of genes such as cyclin D1 and N-cadherin...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344086/correlation-of-structure-function-and-protein-dynamics-in-gh7-cellobiohydrolases-from-trichoderma-atroviride-t-reesei-and-t-harzianum
#6
Anna S Borisova, Elena V Eneyskaya, Suvamay Jana, Silke F Badino, Jeppe Kari, Antonella Amore, Magnus Karlsson, Henrik Hansson, Mats Sandgren, Michael E Himmel, Peter Westh, Christina M Payne, Anna A Kulminskaya, Jerry Ståhlberg
Background: The ascomycete fungus Trichoderma reesei is the predominant source of enzymes for industrial conversion of lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH) TreCel7A constitutes nearly half of the enzyme cocktail by weight and is the major workhorse in the cellulose hydrolysis process. The orthologs from Trichoderma atroviride (TatCel7A) and Trichoderma harzianum (ThaCel7A) show high sequence identity with TreCel7A, ~ 80%, and represent naturally evolved combinations of cellulose-binding tunnel-enclosing loop motifs, which have been suggested to influence intrinsic cellobiohydrolase properties, such as endo-initiation, processivity, and off-rate...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29343529/quarter-descemet-membrane-endothelial-keratoplasty-quarter-dmek-for-fuchs-endothelial-corneal-dystrophy-6-months-clinical-outcome
#7
Vasiliki Zygoura, Lamis Baydoun, Lisanne Ham, Vincent J A Bourgonje, Korine van Dijk, Jessica T Lie, Isabel Dapena, Silke Oellerich, Gerrit R J Melles
BACKGROUND/AIM: To assess the clinical outcome of the first series of Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK), a potential hybrid technique between 'descemetorhexis only' and conventional, circular DMEK. METHODS: Prospective interventional case series at a tertiary referral centre. Twelve eyes of 12 patients with central Fuchs endothelial corneal dystrophy underwent Quarter-DMEK, that is, transplantation of one quadrant of a full-diameter DMEK graft, and were evaluated for best-corrected visual acuity (BCVA), endothelial cell density (ECD) and complications up to 6 months postoperatively...
January 17, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29343509/efficacy-and-safety-of-tregalizumab-in-patients-with-rheumatoid-arthritis-and-an-inadequate-response-to-methotrexate-results-of-a-phase-iib-randomised-placebo-controlled-trial
#8
Ronald F van Vollenhoven, Edward Clark Keystone, Vibeke Strand, Cesar Pacheco-Tena, Jiří Vencovský, Frank Behrens, Arthur Racewicz, Daniela Zipp, Faiza Rharbaoui, Ralf Wolter, Luise Knierim, Rainer Schmeidl, Xuefei Zhou, Silke Aigner, Benjamin Dälken, Andrea Wartenberg-Demand
OBJECTIVE: To evaluate the efficacy, biological activity and safety of tregalizumab in patients with active rheumatoid arthritis (RA) and an inadequate response to methotrexate (MTX). METHODS: 321 patients were randomised (1:1:1:1) to placebo or tregalizumab 25, 100 or 200 mg once-weekly subcutaneously in addition to MTX treatment. Responders at week 12 continued the same treatment, and non-responders at week 12 were escalated to the next higher tregalizumab dose level or re-randomised from placebo to active treatment...
January 17, 2018: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29340944/persons-with-disability-social-deprivation-and-an-emergency-medical-admission
#9
Seán Cournane, Richard Conway, Declan Byrne, Deirdre O'Riordan, Bernard Silke
BACKGROUND: The community level of disability and social deprivation may result in an emergency hospitalisation; we have examined the annual admission incidence rate for emergency medical conditions in relation to the community prevalence of such factors. METHODS: All emergency medical admissions (96,305 episodes in 50,612 patients) within the institution's catchment area were examined between 2002 and 2016. The frequency of disability, level of full-time carers and unemployment for the 74 electoral divisions of the catchment area was regressed against admission rates; incidence rate ratios (IRR) were calculated using truncated Poisson regression...
January 16, 2018: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29340756/differential-effect-of-a-patient-education-transition-intervention-in-adolescents-with-ibd-vs-diabetes
#10
Silke Schmidt, Henriette Markwart, Franziska Bomba, Holger Muehlan, Annette Findeisen, Martina Kohl, Ingo Menrath, Ute Thyen
Patient education programs (PEPs) to improve disease management are part of standard and regular treatment in adolescents with diabetes. In Germany, youth with inflammatory bowel disease (IBD) receive individual counseling but not PEPs in group settings. Generic PEPs have been developed in order to improve transition from child-centered to adolescent health services. The aim of the study was to investigate the effects of a transition-oriented PEP on quality of life (QoL) and self-management in young patients with IBD (PEP naive), compared to patients with diabetes (familiar with PEPs)...
January 16, 2018: European Journal of Pediatrics
https://www.readbyqxmd.com/read/29339287/scalable-fabrication-of-sulfated-silk-fibroin-nanofibrous-membranes-for-efficient-lipase-adsorption-and-recovery
#11
Shixiong Yi, Fangyin Dai, Yuehan Wu, Cunyi Zhao, Yang Si, Gang Sun
Fabricating adsorptive materials for fast and high efficient adsorption of enzymes is critical to match the great demands for separation and recovery of enzymes used as biocatalysts. However, it has proven extremely challenging. Here, we report a cost-effective strategy to construct the sulfated group surface-functionalized silk fibroin nanofibrous membranes (SS-SFNM) under mild conditions for positively charged Candida rugosa lipase adsorption. The naturally abundant silk is thus reconstructed into nanofibrous membranes with tunable surface functions...
January 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29339078/analytical-and-pre-analytical-performance-characteristics-of-a-novel-cartridge-type-blood-gas-analyzer-for-point-of-care-and-laboratory-testing
#12
Matthijs Oyaert, Tom Van Maerken, Silke Bridts, Silvi Van Loon, Heleen Laverge, Veronique Stove
BACKGROUND: Point-of-care blood gas test results may benefit therapeutic decision making by their immediate impact on patient care. We evaluated the (pre-)analytical performance of a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (Werfen), for the determination of pH, partial carbon dioxide pressure (pCO2), partial oxygen pressure (pO2), sodium (Na+), potassium (K+), chloride (Cl-), ionized calcium (iCa2+), glucose, lactate, and total hemoglobin (tHb). METHODS: Total imprecision was estimated according to the CLSI EP5-A2 protocol...
January 12, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29338625/delivery-of-chemotherapeutics-using-spheres-made-of-bioengineered-spider-silks-derived-from-masp1-and-masp2-proteins
#13
Katarzyna Jastrzebska, Anna Florczak, Kamil Kucharczyk, Yinnan Lin, Qin Wang, Andrzej Mackiewicz, David L Kaplan, Hanna Dams-Kozlowska
AIM: Analysis of the properties and chemotherapeutics delivery potential of spheres made of bioengineered spider silks MS1 and MS2. MATERIALS & METHODS: MS1 and MS2 derived from Nephila clavipes dragline silks - MaSp1 and MaSp2, respectively - formed spheres that were compared in terms of physicochemical properties, cytotoxicity and loading/release of chemotherapeutics. RESULTS: MS2 spheres were more dispersed, smaller, of solid core, of higher beta-sheet structure content, and of opposite (negative) charge than MS1 spheres...
January 17, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29338201/self-assembly-of-spider-silk-fusion-proteins-comprising-enzymatic-and-fluorescence-activity
#14
Martin Humenik, Madeleine Mohrand, Thomas Scheibel
The recombinant spider silk protein eADF4(C16) was genetically fused either with esterase 2 (EST2) or green fluorescent protein (GFP). The fusions EST-eADF4(C16) and GFP-eADF4(C16) were spectroscopically investigated and showed native structures of EST and GFP. The structural integrity was confirmed by the enzymatic activity of EST and the fluorescence of GFP. The spider silk moiety retained its intrinsically unstructured conformation in solution and the self-assembly into either nanofibrils or nanoparticles could be controlled by the concentration of phosphate...
January 16, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29336437/ge12-fe-co-3-8-%C3%AE-i-4-a-germanium-iron-cluster-with-ge4-ge2-and-ge-units
#15
Silke Wolf, Wim Klopper, Claus Feldmann
The germanium-iron cluster Ge12{Fe(CO)3}8(μ-I)4 was prepared by reacting GeI4 and Fe2(CO)9 in [BMIm]Cl/AlCl3 as ionic liquid (BMIm: 1-butyl-3-methylimidazolium). The cluster contains a Ge12Fe8 metal core consisting of a central Ge4 rectangle, two Ge2 pairs, and four single Ge atoms. These Ge units are interconnected by Fe atoms to a real Ge-Fe cluster. According to DFT calculations, Hund's localization criterion is fulfilled for the whole cluster if we assume three different atomic charges on the Ge4, Ge2, and Ge units...
January 16, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29336177/in-vivo-assessment-of-a-nanofibrous-silk-tube-as-nerve-guide-for-sciatic-nerve-regeneration
#16
Maryam Ebrahimi, Jafar Ai, Esmaeil Biazar, Somayeh Ebrahimi-Barough, Arash Khojasteh, Meysam Yazdankhah, Siavash Sharifi, Arman Ai, Saeed Heidari-Keshel
A nanofibrous silk nerve conduit has been evaluated for its efficiency based on the promotion of peripheral nerve regeneration in rats. The designed tubes with or without Schwann cells were implanted into a 10 mm gap in the sciatic nerves of the rats. Four months after the surgery, the regenerated nerves were monitored and evaluated by macroscopic assessments and histology. The results demonstrated that the nanofibrous grafts, especially in the presence of Schwann cells, enabled reconstruction of the rat sciatic nerve trunk with a restoration of nerve continuity and formation of nerve fibres with myelination...
January 16, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29336151/ultra-robust-biochips-with-metal-organic-framework-coating-for-point-of-care-diagnosis
#17
Congzhou Wang, Lu Wang, Sirimuvva Tadepalli, Jeremiah J Morrissey, Evan D Kharasch, Rajesh R Naik, Srikanth Singamaneni
Most biosensors relying on antibodies as recognition elements fail in harsh environment conditions such as elevated temperatures, organic solvents or proteases because of antibody denaturation, and require strict storage conditions with defined shelf-life, thus limiting their applications in point-of-care and resource-limited settings. Here, a metal-organic framework (MOF) encapsulation is utilized to preserve the biofunctionality of antibodies conjugated to nanotransducers. This study investigates several parameters of MOF coating (including growth time, surface morphology, thickness and precursor concentrations) that determine the preservation efficacy against different protein denaturing conditions in both dry and wet environments...
January 16, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29335199/3-d-mineralized-silk-fibroin-polycaprolactone-composite-scaffold-modified-with-polyglutamate-conjugated-with-bmp-2-peptide-for-bone-tissue-engineering
#18
Jingjing Luo, Haitao Zhang, Jiang Zhu, Xiaokang Cui, Junjiu Gao, Xin Wang, Jie Xiong
In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated with BMP-2 peptide onto silk fibroin (SF)/polycaprolactone (PCL) (SF/PCL) blend nanofibers. The morphology, composition, and mineralisation, were confirmed by FE-SEM, XRD, and FT-IR spectroscopy...
December 28, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29334455/modulating-the-molybdenum-coordination-sphere-of-escherichia-coli-trimethylamine-n-oxide-reductase
#19
Paul Kaufmann, Benjamin R Duffus, Biljana Mitrova, Chantal Iobbi-Nivol, Christian Teutloff, Manfred Nimtz, Lothar Jänsch, Ulla Wollenberger, Silke Leimkühler
The well-studied enterobacterium Escherichia coli present in the human gut is able to reduce TMAO to trimethylamine (TMA) during anaerobic respiration. The TMAO reductase TorA is a monomeric, bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor-containing enzyme belonging to the dimethylsulfoxide (DMSO) reductase family of molybdoenzymes. We report on a system for the in vitro reconstitution of TorA with molybdenum cofactors (Moco) from different sources. Higher TMAO reductase activities for TorA were obtained when using Moco-sources containing a sulfido ligand at the molybdenum atom...
January 15, 2018: Biochemistry
https://www.readbyqxmd.com/read/29334255/ngs-based-methylation-profiling-differentiates-tcf3-hlf-and-tcf3-pbx1-positive-b-cell-acute-lymphoblastic-leukemia
#20
Priyadarshini Kachroo, Silke Szymczak, Femke-Anouska Heinsen, Michael Forster, Jörn Bethune, Georg Hemmrich-Stanisak, Lewis Baker, Martin Schrappe, Martin Stanulla, Andre Franke
AIM: To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. MATERIALS & METHODS: Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and after remission and analyzed using reduced representation bisulfite sequencing and RNA-sequencing. RESULTS: We identified 7000 differentially methylated CpG sites between subtypes, of which 78% had lower methylation levels in TCF3-HLF...
January 15, 2018: Epigenomics
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